33 results about "Oryzalin" patented technology

Dinitroaniline compounds useful for the treatment of diseases caused by parasitic protozoa in subjects in need of such treatment. The compounds are particularly useful in the treatment of leishmaniasis. The compounds are preferably less cytotoxic to normal cells than oryzalin. Also provided are methods of treating subjects having diseases caused by parasitic protozoa, preferably humans. The method comprising administering a therapeutically effective amount of a dinitroaniline compound of the present invention to a subject in need of such treatment

The invention discloses a method for culturing triploid petunia. The method comprises the following steps: selecting a diploid petunia combination with strong heterosis as a crossing parent, selecting proper flower buds in the flowering period of a male parent, carrying out chemical mutagenesis by adopting mixed liquid of colchicine, oryzalin and trifluralin to generate 2n pollens, pollinating the 2n pollens to a diploid female parent stigma to obtain F1 hybridized seeds, and hybridizing n pollens the male parent of which is not subjected to chemical mutagenesis with the female parent to obtain contrast diploid F1 hybrid seeds. The adult plants developed from the F1 hybridized seeds are compared with the contrast diploid F1 hybridized filial generations in the aspects of agronomic trait and anatomy, and on this basis, the number of chromosomes can be detected so as to finally determine the triploid plant. According to the method disclosed by the invention, the breeding period is short, the operation is convenient, the mutagenesis efficiency is high and the application prospect is good.

The invention discloses a method for inducing polyploid miscanthus sinensis. According to the invention, polyploid mutation is carried out by utilizing a miscanthus sinensis sprout point callus; a mutagenic agent is a water solution which contains Ca<2+> and mannitol, and the water solution also comprises oryzalin or nocodazole which is already subjected to filter sterilization; and finally obtained polyploid mutation rate can achieve 35.19% and a period is 2-3 months through polyploid identification. Compared with conventional breeding, the method disclosed by the invention greatly shortens the mutation time, greatly enhances the mutation rate and greatly reduces the cost; and the method disclosed by the invention provides a new way for selectively breeding a new miscanthus sinensis species.

The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

The invention discloses nitroreductase genes lnr as well as coded proteins and application thereof. The nitroreductase genes lnr has a nucleotide sequence of SEQ ID NO.1, has the full length of 609bp,is capable of coding 202 amino acids, and has an amino acid sequence of SEQ ID NO.2. The LNR is capable of performing catalytic reduction on the nitro group at the site C-6 of a pendimethalin benzenering to produce 2-nitro-6-amino-N-(1-ethylpropyl)-3,4-dimethyl aniline, and further performing catalytic reduction on two symmetrical nitro groups on the benzene ring of butralin, oryzalin and trifluralin, but the catalytic efficiency is obviously higher than that of PNR. The LNR has a broad substrate spectrum, and has wide application prospects in the aspect of eliminating environmental contamination and remediation caused by nitro-aromatic herbicide residues.

The invention discloses a method for rapidly obtaining herba erigernotis triploid. The method comprises the following steps: processing plump herba erigernotis seeds picked in the same year with a water solution of a cell microtubule inhibitor Oryzalin to induce the chromosome doubling of the germ growing point cells; continuously carrying out two times of leaf air hole identification in the seedling stage and the squaring stage, carrying out rapid screening so as to obtain tetraploid variant plants; and hybridizing the tetraploid variant plants with diploid plants (namely fusing egg cells having doubled chromosome with normal sperm cells) so as to obtain ploidy-homozygous triploid. The method utilizes the fusion of single cells (egg cells) with single cells (sperm cells) during the fertilization process of sexual reproduction of angiosperm, simultaneously achieves the ploidy purification of plant chromosome and obtaining of triploid, avoids a large amount of tedious work on purifying the ploidy of the tetraploid variant plants, and provides technical support for cultivating abundant herba erigernotis triploid novel germplasm and breeding triploid novel species.

The invention relates to technology for synthesizing oryzalin by a trichloromethyl carbonic ether method, which relates to technology for synthesizing herbicide, namely the oryzalin. The technology is to take 3,5-binitro-4-di-n-propylamine benzene sulfonate as a raw material, use trichloromethyl carbonic ether to perform acyl chlorination in an inert solvent, and perform aminolysis to obtain crude oryzalin medicines. The innovation of the technology is to adopt the trichloromethyl carbonic ether instead of commonly used phosphorus oxychloride as an acyl chlorination agent, adopt dichloroethane to replace toluene, and finally use an ammonia liquid for direct aminolysis. The technology has the characteristics of cheap and easily obtained raw materials, safe, simple and convenient operation, high process yield, good product quality and small wastewater amount.

An herbicidal synergistic composition containing (a) penoxsulam and (b) oryzalin provides improved post-emergence herbicidal weed control in tree and vine crops, turf, sugar cane, range and pasture, parks and alleyways, and IVM.

The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.

The present invention relates to an emulsifiable concentrate comprising an active ingredient being a dinitroaniline compound, preferably selected from the group consisting of pendimethalin, trifluralin, benfluralin, butralin, dinitramine, ethalfluralin, fluazinam, fluchloralin, flumetralin, oryzalin, prodiamine and mixtures thereof. The emulsifiable concentrate of the invention avoids especially crystallization at low temperature when the concentration of the active ingredient is high.

The invention discloses a method for inducing a polyploid of momordica grosvenori. The method comprises the steps of dipping a pollinated momordica grosvenori female flower by using Oryzalin; and then washing and relieving the dipped flower; and inducing doubling of zygotes by using a novel chemical inducer Oryzalin so as to obtain the polyploid. According to the invention, the chimera problem of the polyploid of the momordica grosvenori is solved, the inducing operation procedures are simplified, the inducing efficiency of the polyploid of the momordica grosvenori is increased, and convenience is obtained in actual applications.

The invention discloses a millettia speciosa champ polyploidy induction method. The method comprises the following steps of pollinating millettia speciosa champ flowers, treating the pollinated millettia speciosa champ flowers with oryzalin, when fruits are ripe, harvesting the fruits, performing sowing and seedling raising and identifying chromosomes. The polyploid induction method provided by the invention has the characteristics of quick action, low cost, simplicity in operation, convenience in implementation, high induction efficiency, no chimeras and the like.

The invention discloses two polyploid centella asiatica varieties and a cultivation method thereof. The cultivation method comprises the following steps of 1, selecting terminal buds of a diploid centella asiatica adult plant, and carrying out breeding of a tissue cultured seedling or callus tissue, 2, carrying out chromosome doubling induction culture and differentiation culture of tissue subcultured seedling or callus tissue by oryzalin, 3, cutting down novel buds obtained by the differentiation culture and carrying out cluster bud culture, 4, carrying out chromosome doubling identification of the cultured complete plants having roots, stems and leaves, screening the tetraploid plant, and planting the tetraploid plant to obtain the tetraploid centella asiatica variety, 5, carrying out artificial emasculation and pollination hybridization of the tetraploid plant and the diploid plant to obtain hybrid seeds (female parent 4N*male parent 2N or male parent 4N* female parent 2N, wherein F1 is 3x), and 6, breeding the hybrid seeds into complete plants, planting the plants, carrying out chromosome number detection and screening the triploid centella asiatica variety.

The invention discloses a culturing method capable of effectively reducing the reverse mutation rate of horseradish polyploid plants. The method comprises the following steps: disinfecting horseradish seeds and sequentially culturing by a mixed solution of 5-aminuracil and propylene glycol, a mixed solution of oryzalin and propyzamide, and a mixed solution of colchicine and fumiron. With the culturing method, the reverse mutation rate of polyploid seedlings cultured by obtained polyploid seeds can be effectively reduced; the culturing method is a new way for rapidly culturing good horseradish varieties.

The present invention relates to an emulsifiable concentrate comprising an active ingredient being a dinitroaniline compound, preferably selected from the group consisting of pendimethalin, trifluralin, benfluralin, butralin, dinitramine, ethalfluralin, fluazinam, fluchloralin, flumetralin, oryzalin, prodiamine and mixtures thereof. The emulsifiable concentrate of the invention avoids especially crystallization at low temperature when the concentration of the active ingredient is high.

The invention provides a cultivation technology for anoectochilus formosanus. The cultivation technology comprises the following steps: transplanting whole field diploid anoectochilus formosanus plants with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus formosanus plants at the flowering phase, and picking after fruits ripen to obtain fruits of diploidanoectochilus formosanus; planting seed embryos of the diploid anoectochilus formosanus fruits into a germination culture medium for culturing to obtain protocorms, wherein the germination culture medium is prepared from MS, 0.9 to 1 mg / L of BA, 0.8 to 1 mg / L of KT, 0.08 to 0.12 mg / L of NAA, 25 to 30 mg / L of saccharose, 5 to 7 mg / L of agar and 20 to 22 mg / L of banana; carrying out dark culture onthe protocorms for 4 days and then soaking treatment in 8 to 10 [mu]mol / L of oryzalin solution for 10 to 36 hours; putting the soaked protocorms in a cluster culture medium for continuously culturingto obtain cluster buds, wherein the cluster culture medium is identical with the germination culture medium. Gravity progressive enhancement is adopted for stimulating in the rooting culture process,so that the development degree of root systems of polyploidy anoectochilus formosanus seedlings can be remarkably enhanced, and transplanting survival rate and rapid growth probability are improved.

The invention discloses a method for inducing tetraploid garlic by using oryzalin. According to the method, the oryzalin is selected as a mutagenic agent for inducing the tetraploid garlic, an optimal mutagenizing method is adopted, and the best effect of inducing tetraploid garlic is obtained through research, and thus germplasm resource reserve is provided for germplasm innovation and breeding of new variety of the garlic.

The invention discloses a method for rapidly obtaining a tripod of siraitia grosvenorii. The method comprises the following steps: soaking a female flower at a reduction mitosis stage by using a novel chromosome doubling agent Oryzalin with high induction efficiency and low toxicity according to the floral traits of female flower megaspore mother cells in reduction mitosis to inhibit normal separation of chromosomes in reduction mitosis and induce the production of unreduced female gametes; and after the female flower to be treated blooms, pollinating by using diploid male plant pollen to obtain a tripod descendant. By adopting the method, the technical problems of long cultivation time, the need of screening unreduced gametes during cultivation of the siraitia grosvenorii tripod by manual induction of the unreduced female gametes and the like in the conventional tripod cultivation method of siraitia grosvenorii are solved effectively, the tripod can be successfully obtained conveniently and easily in one growing period, and technical support is provided for the creation of abundant new siraitia grosvenorii tripod germplasms as well as the cultivation of new seedless tripod varieties.

The invention discloses an induction method of Dendrobium polyploidy, comprising the steps of using 60-90 mu M of Oryzalin to soak pollinated Dendrobium megasporocytes and Dendrobium capsules in meiosis phase continuously for 48-72 hours, washing with clear water to remove Oryzalin, aseptically germinating seeds, identifying chromosome, and acquiring a polyploid plant from offspring. The chimera problem of Dendrobium polyploidy is solved, the problem that conventional triploid cultivation takes long time and is complex to operate is solved, and a triploid can be successfully cultivated conveniently within one growth period.

The invention discloses a method for rapidly obtaining herba erigernotis triploid. The method comprises the following steps: processing plump herba erigernotis seeds picked in the same year with a water solution of a cell microtubule inhibitor Oryzalin to induce the chromosome doubling of the germ growing point cells; continuously carrying out two times of leaf air hole identification in the seedling stage and the squaring stage, carrying out rapid screening so as to obtain tetraploid variant plants; and hybridizing the tetraploid variant plants with diploid plants (namely fusing egg cells having doubled chromosome with normal sperm cells) so as to obtain ploidy-homozygous triploid. The method utilizes the fusion of single cells (egg cells) with single cells (sperm cells) during the fertilization process of sexual reproduction of angiosperm, simultaneously achieves the ploidy purification of plant chromosome and obtaining of triploid, avoids a large amount of tedious work on purifying the ploidy of the tetraploid variant plants, and provides technical support for cultivating abundant herba erigernotis triploid novel germplasm and breeding triploid novel species.

The invention belongs to the technical field of plant identification and discloses a tabletting method of eucalyptus chromosome.The tabletting method includes following steps: taking materials, pretreating, fixing, dissociating, dyeing, tabletting and performing microscopic examination.In the tabletting method, oryzalin is adopted as a pretreatment agent, so that cytotoxic effect is reduced obviously when cell mitosis is blocked; cells can be in normal mitosis, so that during fixing, number of accumulated metaphase cells is large, chromosome tabletting success probability is increased, and stability is improved.Oryzalin is low in cost and far lower than colchicine in toxicity; in the tabletting process, environment pollution can be reduced and degree and risk of causing damage to people are lowered.