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35 results about "Oryzalin" patented technology

Oryzalin is an herbicide of the dinitroaniline class. It acts through the disruption (depolymerization) of microtubules, thus blocking anisotropic growth of plant cells. It can also be used to induce polyploidy in plants as an alternative to colchicine.

Method for rapidly obtaining triploid of siraitia grosvenorii

The invention discloses a method for rapidly obtaining a tripod of siraitia grosvenorii. The method comprises the following steps: soaking a female flower at a reduction mitosis stage by using a novel chromosome doubling agent Oryzalin with high induction efficiency and low toxicity according to the floral traits of female flower megaspore mother cells in reduction mitosis to inhibit normal separation of chromosomes in reduction mitosis and induce the production of unreduced female gametes; and after the female flower to be treated blooms, pollinating by using diploid male plant pollen to obtain a tripod descendant. By adopting the method, the technical problems of long cultivation time, the need of screening unreduced gametes during cultivation of the siraitia grosvenorii tripod by manual induction of the unreduced female gametes and the like in the conventional tripod cultivation method of siraitia grosvenorii are solved effectively, the tripod can be successfully obtained conveniently and easily in one growing period, and technical support is provided for the creation of abundant new siraitia grosvenorii tripod germplasms as well as the cultivation of new seedless tripod varieties.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Method for culturing triploid petunia

The invention discloses a method for culturing triploid petunia. The method comprises the following steps: selecting a diploid petunia combination with strong heterosis as a crossing parent, selecting proper flower buds in the flowering period of a male parent, carrying out chemical mutagenesis by adopting mixed liquid of colchicine, oryzalin and trifluralin to generate 2n pollens, pollinating the 2n pollens to a diploid female parent stigma to obtain F1 hybridized seeds, and hybridizing n pollens the male parent of which is not subjected to chemical mutagenesis with the female parent to obtain contrast diploid F1 hybrid seeds. The adult plants developed from the F1 hybridized seeds are compared with the contrast diploid F1 hybridized filial generations in the aspects of agronomic trait and anatomy, and on this basis, the number of chromosomes can be detected so as to finally determine the triploid plant. According to the method disclosed by the invention, the breeding period is short, the operation is convenient, the mutagenesis efficiency is high and the application prospect is good.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY

Method for inducing polyploid miscanthus sinensis

The invention discloses a method for inducing polyploid miscanthus sinensis. According to the invention, polyploid mutation is carried out by utilizing a miscanthus sinensis sprout point callus; a mutagenic agent is a water solution which contains Ca<2+> and mannitol, and the water solution also comprises oryzalin or nocodazole which is already subjected to filter sterilization; and finally obtained polyploid mutation rate can achieve 35.19% and a period is 2-3 months through polyploid identification. Compared with conventional breeding, the method disclosed by the invention greatly shortens the mutation time, greatly enhances the mutation rate and greatly reduces the cost; and the method disclosed by the invention provides a new way for selectively breeding a new miscanthus sinensis species.
Owner:HUNAN AGRICULTURAL UNIV

Breeding Method for Tetraploid Ricinus Communis

The present invention provides a breeding method for tetraploid ricinus communis. The method comprises: collecting a ricinus communis germplasm resource, performing morphologic character analysis and chromosome ploidy identification, selecting good diploid varieties, carrying out mutagenesis by using colchicine and oryzalin; performing chromosome ploidy identification on F1 generation seeds, selecting homozygous tetraploids, and performing economic character analysis and identification on the F2 generation, so as to breed good tetraploid ricinus communis.
Owner:TIANJIN NANKAI UNIV CASTOR ENG SCI & TECH +1

Strawberry chromosome doubling method and rapid identification method for used ploidy

The invention discloses a rapid identification method for the ploidy of a strawberry chromosome. The rapid identification method comprises the steps: taking a mutation-induced strawberry obtained by inducing a wild forest strawberry as a test material and a diploid wild forest strawberry as a contrast; preparing a test material stained cell nucleus suspension from the test material, and preparinga contrast stained cell nucleus suspension from a contrasted leaf; and detecting the two suspensions on a flow cytometer, analyzing the ploidy of the test material by utilizing a histogram for the relative content of DNA, and screening a ploidy-variant individual plant. The invention simultaneously provides a strawberry chromosome doubling method realized by using the rapid identification method for the ploidy of the strawberry chromosome, and the chromosome of the diploid wild forest strawberry is doubled by using oryzalin in the method. By using the method disclosed by the invention, a strawberry doubling progeny can be effectively obtained.
Owner:HANGZHOU ACAD OF AGRI SCI

Nitroreductase gene lnr as well as coded proteins and application thereof

The invention discloses nitroreductase genes lnr as well as coded proteins and application thereof. The nitroreductase genes lnr has a nucleotide sequence of SEQ ID NO.1, has the full length of 609bp,is capable of coding 202 amino acids, and has an amino acid sequence of SEQ ID NO.2. The LNR is capable of performing catalytic reduction on the nitro group at the site C-6 of a pendimethalin benzenering to produce 2-nitro-6-amino-N-(1-ethylpropyl)-3,4-dimethyl aniline, and further performing catalytic reduction on two symmetrical nitro groups on the benzene ring of butralin, oryzalin and trifluralin, but the catalytic efficiency is obviously higher than that of PNR. The LNR has a broad substrate spectrum, and has wide application prospects in the aspect of eliminating environmental contamination and remediation caused by nitro-aromatic herbicide residues.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for rapidly obtaining herba erigernotis triploid

The invention discloses a method for rapidly obtaining herba erigernotis triploid. The method comprises the following steps: processing plump herba erigernotis seeds picked in the same year with a water solution of a cell microtubule inhibitor Oryzalin to induce the chromosome doubling of the germ growing point cells; continuously carrying out two times of leaf air hole identification in the seedling stage and the squaring stage, carrying out rapid screening so as to obtain tetraploid variant plants; and hybridizing the tetraploid variant plants with diploid plants (namely fusing egg cells having doubled chromosome with normal sperm cells) so as to obtain ploidy-homozygous triploid. The method utilizes the fusion of single cells (egg cells) with single cells (sperm cells) during the fertilization process of sexual reproduction of angiosperm, simultaneously achieves the ploidy purification of plant chromosome and obtaining of triploid, avoids a large amount of tedious work on purifying the ploidy of the tetraploid variant plants, and provides technical support for cultivating abundant herba erigernotis triploid novel germplasm and breeding triploid novel species.
Owner:INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI

Method for cultivating pelargonium graveolens polyploids

The invention provides a method for cultivating pelargonium graveolens polyploids, and belongs to the technical field of flower cultivation. The method comprises the steps of 1) preparation, wherein an inducing solution is obtained by dissolving an inducing aid in a glucose solution; 2) inducing, wherein pelargonium graveolens seeds are placed in the inducing solution for inducing culture; 3) germination, wherein the induced pelargonium graveolens seeds are transferred to a germination culture medium for culture to obtain polyploid seedlings; 4) expanding propagation, wherein the polyploid seedlings are selected and transferred into a proliferation culture medium for expanding propagation and identification; 5) rooting, wherein plants which are identified to be polyploids are transferred into a rooting culture medium for rooting culture to obtain seedlings, and the seedlings are transplanted. The method has the advantages that compared with a mode of only using oryzalin, the inducing success rate is significantly increased, the polyploid traits are stable, the increase of the content of chimeras in the polyploids is also promoted, pelargonium graveolens with more excellent and specific color, shape, taste and the like is obtained accordingly, the roots of the seedlings are strong after cultivation, and the survival rate after transplanting is high.
Owner:金华市飞凌生物科技有限公司

Tabletting method of eucalyptus chromosome

The invention belongs to the technical field of plant identification and discloses a tabletting method of eucalyptus chromosome.The tabletting method includes following steps: taking materials, pretreating, fixing, dissociating, dyeing, tabletting and performing microscopic examination.In the tabletting method, oryzalin is adopted as a pretreatment agent, so that cytotoxic effect is reduced obviously when cell mitosis is blocked; cells can be in normal mitosis, so that during fixing, number of accumulated metaphase cells is large, chromosome tabletting success probability is increased, and stability is improved.Oryzalin is low in cost and far lower than colchicine in toxicity; in the tabletting process, environment pollution can be reduced and degree and risk of causing damage to people are lowered.
Owner:RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY

Carnation new specie cultivation method

The invention discloses a carnation new specie cultivation method, and relates to the technical field of plant culture. The method includes the steps: culturing a carnation explant, placing the explant into a culture medium containing mutagenic agents with high concentration to induce the explant after a callus tissue is formed; transferring the explant into the culture medium containing mutagenicagents with lower concentration to perform multiplication; transferring the explant into the culture medium without mutagenic agents with lower concentration to perform rooting culture; transplantingrotting seedlings to obtain transplanting seedlings, culturing the transplanting seedlings into plates, identifying the plants by morphology, cytology and chromosome number, and determining an selecting carnation new specie. The mutagenic agents comprise mixed solution of colchicine, oryzalin dimethyl sulfoxide and crown ether. The invention provides a carnation new specie cultivation method. Themethod is high in operability and repeatability, directed variation can be achieved, chimeras are decreased, polyploid variation rate is increased, vitrification is reduced, subpolyploids are stable,identifying time is shortened, and the survival rate of the mutant seedlings is high.
Owner:中国科学院昆明植物研究所海盐工程技术中心

Process for synthesizing ryzalin by trichloromethyl carbonate method

The invention relates to technology for synthesizing oryzalin by a trichloromethyl carbonic ether method, which relates to technology for synthesizing herbicide, namely the oryzalin. The technology is to take 3,5-binitro-4-di-n-propylamine benzene sulfonate as a raw material, use trichloromethyl carbonic ether to perform acyl chlorination in an inert solvent, and perform aminolysis to obtain crude oryzalin medicines. The innovation of the technology is to adopt the trichloromethyl carbonic ether instead of commonly used phosphorus oxychloride as an acyl chlorination agent, adopt dichloroethane to replace toluene, and finally use an ammonia liquid for direct aminolysis. The technology has the characteristics of cheap and easily obtained raw materials, safe, simple and convenient operation, high process yield, good product quality and small wastewater amount.
Owner:江苏乐斯化学有限公司 +2

Method for inducing tetraploid garlic by using oryzalin

The invention discloses a method for inducing tetraploid garlic by using oryzalin. According to the method, the oryzalin is selected as a mutagenic agent for inducing the tetraploid garlic, an optimal mutagenizing method is adopted, and the best effect of inducing tetraploid garlic is obtained through research, and thus germplasm resource reserve is provided for germplasm innovation and breeding of new variety of the garlic.
Owner:VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI

Method for in-vitro inducing tetraploid watermelon by utilizing Oryzalin herbicide

InactiveCN1826888AOvercome the disadvantage of low induction frequencyBiocidePlant growth regulatorsGreenhouseSterile water
The invention discloses a method of isolated inducing watermelon tetraploid using Ou laling herbicide, comprising the following steps: (1) taking cotyledon near hypocotyls of four-day life as explant; (2) pre-culturing the explant in enrichment culture medium for 4.5-5.5 days; (3) immersing the pre-cultured explant in liquid inductive culture medium, shocking in thermostatic oscillator and then treating under dark conditions for 11-13 hours; (4) washing the treated explant with sterilized water, replanting it to enrichment culture medium for 25 days and getting regenerating bud; (5) root culturing the regenerative bud, then training and replanting it to greenhouse and getting the watermelon regenerative plant with tetraploid of high frequency.
Owner:ZHEJIANG UNIV

Breeding method of tetraploid non-heading Chinese cabbage

The invention discloses a breeding method of a tetraploid non-heading Chinese cabbage. According to the breeding method, a cell microtubule inhibitor oryzalin mixed water solution is dropwise added onthe growth points of seedlings of a non-heading Chinese cabbage so as to double the cell chromosomes; in the seedling period, pore identification is performed, in the blossoming period, pollen identification is carried out, and finally tetraploid plants are screened out through the identification of flow cytometer. At the same time, tween 80 and Silwet L-77 are added into the oryzalin water solution; the tween 80 can increase the solubility of oryzalin and the stability of the oryzalin water solution, the Silwet L-77 can greatly reduced the water surface tension, thus the solution can be welladhered on the surface of leaves, and the tetraploid induction rate is increased. According to the method, the oryzalin mixed water solution is used to inhibit the formation of spindle fibers in thelater period of cell mitosis, thus chromosome doubling is realized, and technical support is provided for creating abundant novel tetraploid seeds of non-heading Chinese cabbage and novel tetraploid species.
Owner:NANJING AGRICULTURAL UNIVERSITY

Antileishmanial dinitroaniline sulfanomides with activity against parasite tubulin

Dinitroaniline compounds useful for the treatment of diseases caused by parasitic protozoa in subjects in need of such treatment. The compounds are particularly useful in the treatment of leishmaniasis. The compounds are preferably less cytotoxic to normal cells than oryzalin. Also provided are methods of treating subjects having diseases caused by parasitic protozoa, preferably humans. The method comprising administering a therapeutically effective amount of a dinitroaniline compound of the present invention to a subject in need of such treatment.
Owner:THE OHIO STATES UNIV +1

Synergistic herbicidal composition containing penoxsulam and oryzalin

An herbicidal synergistic composition containing (a) penoxsulam and (b) oryzalin provides improved post-emergence herbicidal weed control in tree and vine crops, turf, sugar cane, range and pasture, parks and alleyways, and IVM.
Owner:DOW AGROSCIENCES LLC

Emulsifiable concentrate comprising a dinitroaniline compound

The present invention relates to an emulsifiable concentrate comprising an active ingredient being a dinitroaniline compound, preferably selected from the group consisting of pendimethalin, trifluralin, benfluralin, butralin, dinitramine, ethalfluralin, fluazinam, fluchloralin, flumetralin, oryzalin, prodiamine and mixtures thereof. The emulsifiable concentrate of the invention avoids especially crystallization at low temperature when the concentration of the active ingredient is high.
Owner:RHODIA OPERATIONS SAS

Millettia speciosa champ polyploidy induction method

The invention discloses a millettia speciosa champ polyploidy induction method. The method comprises the following steps of pollinating millettia speciosa champ flowers, treating the pollinated millettia speciosa champ flowers with oryzalin, when fruits are ripe, harvesting the fruits, performing sowing and seedling raising and identifying chromosomes. The polyploid induction method provided by the invention has the characteristics of quick action, low cost, simplicity in operation, convenience in implementation, high induction efficiency, no chimeras and the like.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

A kind of cultivation technique of golden clematis

The invention provides a cultivation technology for anoectochilus formosanus. The cultivation technology comprises the following steps: transplanting whole field diploid anoectochilus formosanus plants with soil into an artificial greenhouse, carrying out artificial pollination on the anoectochilus formosanus plants at the flowering phase, and picking after fruits ripen to obtain fruits of diploidanoectochilus formosanus; planting seed embryos of the diploid anoectochilus formosanus fruits into a germination culture medium for culturing to obtain protocorms, wherein the germination culture medium is prepared from MS, 0.9 to 1 mg / L of BA, 0.8 to 1 mg / L of KT, 0.08 to 0.12 mg / L of NAA, 25 to 30 mg / L of saccharose, 5 to 7 mg / L of agar and 20 to 22 mg / L of banana; carrying out dark culture onthe protocorms for 4 days and then soaking treatment in 8 to 10 [mu]mol / L of oryzalin solution for 10 to 36 hours; putting the soaked protocorms in a cluster culture medium for continuously culturingto obtain cluster buds, wherein the cluster culture medium is identical with the germination culture medium. Gravity progressive enhancement is adopted for stimulating in the rooting culture process,so that the development degree of root systems of polyploidy anoectochilus formosanus seedlings can be remarkably enhanced, and transplanting survival rate and rapid growth probability are improved.
Owner:泉州市金草生物技术有限公司
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