Method for rapidly obtaining triploid of siraitia grosvenorii

A Luo Han Guo and triploid technology, applied in the field of plant genetics and breeding, can solve problems such as cumbersome operating procedures

Inactive Publication Date: 2014-07-23
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Induction of unreduced male gametes requires screening and isolation to obtain unreduced male gametes (diploid large pollen), and then conduct pollination hybridization to exclude normal gametes (normal pollen) and unreduced male gametes for fertilization competition, and the operating procedures are cumbersome

Method used

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  • Method for rapidly obtaining triploid of siraitia grosvenorii
  • Method for rapidly obtaining triploid of siraitia grosvenorii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In the field, select female plants of the Luo Han Guo variety type "Qing Pi Guo" that have no occurrence of diseases and insect pests and grow robustly.

[0019] Entering the budding stage, select and mark the female flower of the meiosis of the megaspore mother cell of the female plant (the length of the ovary is 7.0mm≤L<9.0mm).

[0020] Put the marked female flowers into a 0.5 mL centrifuge tube, add 0.5 mL of a 30 μM Oryzalin aqueous solution, and soak for 3 hours.

[0021] After the treatment, take out the female flowers and wash them with water to remove Oryzalin.

[0022] On the first day after the female flowers were treated with Oryzalin, fresh pollen from diploid male plants was used for artificial pollination.

[0023] When the female flower swells to form a fruit and the color of the fruit changes from green to yellow-green, pick the fruit.

[0024] Strip the seeds from the fruit, manually remove the seed husk, sow and raise seedlings. When the plant ...

Embodiment 2

[0027] In the field, select female plants of the Luo Han Guo variety type "Qing Pi Guo" that have no occurrence of diseases and insect pests and grow robustly.

[0028] Entering the budding stage, select and mark the female flower of the meiosis of the megaspore mother cell of the female plant (the length of the ovary is 7.0mm≤L<9.0mm).

[0029] Put the marked female flowers into a 0.5 mL centrifuge tube, add 0.5 mL of 60 μM Oryzalin aqueous solution, and soak for 9 hours.

[0030] After the treatment, take out the female flowers and wash them with water to remove Oryzalin.

[0031] On the first day after the female flowers were treated with Oryzalin, fresh pollen from diploid male plants was used for artificial pollination.

[0032] When the female flower swells to form a fruit and the color of the fruit changes from green to yellow-green, pick the fruit.

[0033] Strip the seeds from the fruit, manually remove the seed husk, sow and raise seedlings. When the plant has...

Embodiment 3

[0036] In the field, select female plants of the Luo Han Guo variety type "Qing Pi Guo" that have no occurrence of diseases and insect pests and grow robustly.

[0037] Entering the budding stage, select and mark the female flower of the meiosis of the megaspore mother cell of the female plant (the length of the ovary is 7.0mm≤L<9.0mm).

[0038] Put the marked female flowers into a 0.5 mL centrifuge tube, add 0.5 mL of 90 μM Oryzalin aqueous solution, and soak for 6 hours.

[0039] After the treatment, take out the female flowers and wash them with water to remove Oryzalin.

[0040] On the first day after the female flowers were treated with Oryzalin, fresh pollen from diploid male plants was used for artificial pollination.

[0041] When the female flower swells to form a fruit and the color of the fruit changes from green to yellow-green, pick the fruit.

[0042] Strip the seeds from the fruit, manually remove the seed husk, sow and raise seedlings. When the plant ha...

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Abstract

The invention discloses a method for rapidly obtaining a tripod of siraitia grosvenorii. The method comprises the following steps: soaking a female flower at a reduction mitosis stage by using a novel chromosome doubling agent Oryzalin with high induction efficiency and low toxicity according to the floral traits of female flower megaspore mother cells in reduction mitosis to inhibit normal separation of chromosomes in reduction mitosis and induce the production of unreduced female gametes; and after the female flower to be treated blooms, pollinating by using diploid male plant pollen to obtain a tripod descendant. By adopting the method, the technical problems of long cultivation time, the need of screening unreduced gametes during cultivation of the siraitia grosvenorii tripod by manual induction of the unreduced female gametes and the like in the conventional tripod cultivation method of siraitia grosvenorii are solved effectively, the tripod can be successfully obtained conveniently and easily in one growing period, and technical support is provided for the creation of abundant new siraitia grosvenorii tripod germplasms as well as the cultivation of new seedless tripod varieties.

Description

technical field [0001] The invention belongs to the field of plant genetics and breeding, in particular to a technique for cultivating plant triploids, in particular to a method for rapidly obtaining triploids of Luo Han Guo. Background technique [0002] Triploid refers to a plant whose somatic cells have three chromosome sets, and is a type of polyploid. Triploid not only has the great characteristics of polyploid, but also has infertility, which can be used for the cultivation of excellent seedless plants. At present, a triploid seedless watermelon (Citrullus lanatus) has been bred by artificial induction of tetraploids and hybridization between tetraploids and diploids (Li Jianwu, Ma Changsheng, Sun Zhongwei, Zhang Jie. New Seedless Watermelon Varieties' Yuyi Jackfruit'. Acta Horticultural Science, 2005,32,(2):374), Seedless Lycium barbarum (Lycium barbarum) (Li Jian, Wang Jinxiu, Wang Liying, Huang Zhanming. Research on breeding of new seedless Lycium barbarum varietie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 董志渊马小军缪剑华白隆华潘丽梅冯世鑫莫长明
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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