336 results about "Mother cells" patented technology

The invention provides an overexpression porcine co-stimulatory 4-1BB vector and application thereof. PCR (polymerase chain reaction) amplification is performed on a left homologous arm and a right homologous arm of an intron 1 of a rosa26 gene, a 4-1BB regulatory sequence and an OCT4 specific promoter; the left homologous arm, a 4-1BB expression cassette, LoxP locus-contained Cre and Neo expression cassettes, the right homologous arm and negative selection DTA diphtheria toxin are connected in sequence to obtain a 4-1BB homologous recombinant vector p4BOCNDR; the vector and a CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated) targeting vector of sgRNA (small guide ribonucleic acid) containing the intron 1 of the specific targeting porcine rosa26 gene are transferred together into a porcine fetus fibroblast; by taking a positive cell as a donor cell and an oocyte as a recipient cell, a cloned embryo is obtained through a somatic cell nuclear transfer technique; the cloned embryo is transplanted into a porcine uterus for fetation to obtain a transgenic pig integrating a 4-1BB gene at the fixed point of a first intron of the rosa26 gene and automatically deleting a marker gene.

Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.

The invention provides an ergosterin derivative which is obtained by extraction, separation and purification from traditional Chinese medicine ganoderma lucidum spore powder. In the invention, an anti-aging yeast model, namely a K6001 yeast cell and a mutant strain thereof (delta uth1 and delta skn7) prove that the ergosterin derivative influences the gene expression of the UTH1 by regulating the activity of the Skn7 so as to prolong the duplicability life of yeast. The compound is used as a lead compound to optimize the structure, and can be used for preparing medicaments for delaying aging and preventing or/and treating aging diseases. The general structure formula of the ergosterin derivative is as shown in the specification.

The present invention relates to a novel hepatocyte-like cell population derived from hBS cells and to the potential use of such heopatocyte-like cells in e.g. medical treatment, drug screening and toxicity testing. Furthermore, the invention relates to hepatoblast-like cells that may have suitable characteristics so that they can be used for the same applications as the hepatocyte-like cells and that furthermore may be used in in vitro studies of hepatogenesis such as early hepatogenesis or hepato-regenerative disorders. Both the hepatocyte-like and the hepatoblast-like cells according to the invention express drug transporter and/or drug metabolising characteristics either at the gene or protein expression level.

The invention discloses a preparation method of multi-copy high expressed recombined plectasin by pichia pastoris. The method comprises the following steps: a plectasin expressing gene sequence is designed according to preference performance to codon translated by pichia pastoris; the optimized plectasin gene is fused on an alpha-factor signal peptide C terminus of an expression vector pPICZalphaA to construct a single-copy expression vector, the vector comprises a plectasin expression cassette containing a start signal element alcohol oxygen dehydrogenase strong promoter (AOX), alpha-factor signal peptide gene and a plectasin gene fused in C terminus, a stop signal element AOX (TT) and the like. A complementation principle of restriction endonuclease Bg1II and BamHI cohesive end is used to obtain plectasin gene-containing recombinant plasmid of different copy cascade expression cassettes, pichia pastoris is electrotransformed and secreted and expressed plectasin with high efficiency under the methanol induction. The expression level and plectasin gene copy number exist a linear relation. The constructed multi-copy high expressed yeast cells can be used for raising the output and reducing the cost, and is adapted to large scale production of plectasin.

Viability of cancer cells (e.g., glioblastoma cells) can be reduced by administering ABT-751 to the cancer cells and applying an alternating electric field with a frequency between 100 and 400 kHz (e.g., 200 kHz) to the cancer cells. Notably, experiments show that the combination of ABT-751 and the alternating electric field produces synergistic results for certain glioblastoma cell lines.

The invention relates to the technical field of biomedical engineering and particularly relates to a long-term in-vitro culture and directional differentiation system and method for a liver stem cell. The long-term in-vitro culture and directional differentiation system comprises an amplification culture medium with specific chemical components, and a differential medium with specific chemical components, wherein the amplification culture medium is used for carrying out in-vitro culture of a mouse or human liver stem cell, and the differential medium is used for carrying out induced differentiation on the mouse or human liver stem cell to form a matured liver cell. By using the long-term in-vitro culture and directional differentiation system and method, a selectively-amplified liver stem cell in mother cells of the liver can be obtained from a mouse embryonic liver tissue or through human multipotential stem cell differentiation, the liver stem cell can be cultured for more than 20 generations under such a condition, and the stable molecular phenotype of the liver stem cell is maintained. By using the long-term in-vitro culture and directional differentiation system and method, the cultured mouse or human liver stem cell can be further subjected to induced differentiation to form the matured liver cell with functions of secreting albumin, metabolizing urea and the like.

The invention relates to a method of making pluripotent stem cells that does not involve the formation of early preimplantation embryos or fetal tissue. The method has general utility in the production of pluripotent stem cells from many mammalian species but has particular application in man where pluripotent stem cell production can be customized to particular human individual. The method involves the fusion of donor somatic or stem cells (or their karyoplasts) with cytoplasmic, membrane-delimited fragments of mammalian oocytes or zygotes. After the initial genomic reprogramming occurs, the cells can proliferate and thus multiply in vitro yielding a large number of autologous cells for cell therapy application. The result of this process is a cell population genomically identical to the somatic, differentiated cells derived from an individual patient. However, these cells are pluripotent in that upon application of specific growth factors, the cells are capable of differentiating into specific cell types as required by the sought clinical indication.

The embodiment of the invention provides a hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions and a preparation method thereof, and relates to the field ofcosmetics. The hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions has the functions of regulating the secretion of scalp grease, activating hair mother cells and promoting the division of the hair mother cells, transforms a hair follicle resting period into a growing period, makes the hair follicle primordium revived, and can effectively play roles inpreventing hair loss and making hair grow. The hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions at least comprises the following components by the mass percentage: 0.01-1% of pantothenol, 0.01-5% of a plant extract, 0.01-1% of resveratrol and 0.01-1% of menthol.

The invention relates to a screening method for roes of fishes in Acipenseridae, which aims to solve the problem that the evaluating method is mistaken as the detecting method for the quality of the fish roes is lack of overall evaluation. The evaluation method is as follows, firstly, taking out a roe from the fish in Acipenseridae; secondly, preparing roe extracting solution; thirdly, preparing blood serum; fourthly, preparing body fluid; fifth, determining the polarization indexes of mother cells of the roe; sixthly, determining the density of blood serum vitellogenin; seventhly, determining the contents of Fe ions, roe Zn ions, roe VE and roe glutamic-oxaloacetic transaminase; eighthly, determining the contents of ACP and AKP; and ninthly, if the determined indexes simultaneously meet the target values, the remaining roes in the same ovary are mature, and then the screening of the roes of the fishes in Acipenseridae is accomplished. By adopting the invention, the fertilization rate of the sturgeon, the hatchability of the sturgeon and the survival rate of the fry are more than 85%, 95% and 90% respectively. The screening method is used for breeding the fishes in Acipenseridae.

The invention discloses a plant growth conditioner for reducing damage caused by high temperature to rice, and a using method and application of the plant growth conditioner. The conditioner comprises gibberellin and salicylic acid, wherein the weight ratio of gibberellin to salicylic acid is (0.5-1.5):(0.15-0.6). The conditioner can also comprise chitosan oligosaccharide according to a certain ratio. When the conditioner is used, an aqueous solution form is adopted, wherein an aqueous solution is prepared from 50 to 150mg/L of gibberellin, 15 to 60mg/L of salicylic acid and 50 to 200mg/L of chitosan oligosaccharide, and is applied to leaves in a spraying way during the meiosis stage of pollen mother cells of rice, and 30 to 45 liters of aqueous solution is applied to each acre. The plant growth conditioner can be applied to reduce the damage caused by high temperature to rice, and is suitable for popularization and application in a rice culturing region on the middle and lower reaches of Changjiang River, particularly in a region at which the temperature in a flowering period is always high, and high and stable yield of rice is ensured.

Expression of a xylose isomerase in a yeast cell that expresses the chaperonins GroES and GroEL was found to result in enzymatically active xylose isomerase, while there is little to no activity with expression of the bacterial xylose isomerase in a yeast cell lacking GroES and GroEL. A yeast cell expressing xylose isomerase activity, and a complete xylose utilization pathway, provides a yeast cell that can produce a target compound, such as ethanol, butanol, or 1,3-propanediol, using xylose derived from lignocellulosic biomass as a carbon source.

The invention discloses a method for rapidly obtaining a tripod of siraitia grosvenorii. The method comprises the following steps: soaking a female flower at a reduction mitosis stage by using a novel chromosome doubling agent Oryzalin with high induction efficiency and low toxicity according to the floral traits of female flower megaspore mother cells in reduction mitosis to inhibit normal separation of chromosomes in reduction mitosis and induce the production of unreduced female gametes; and after the female flower to be treated blooms, pollinating by using diploid male plant pollen to obtain a tripod descendant. By adopting the method, the technical problems of long cultivation time, the need of screening unreduced gametes during cultivation of the siraitia grosvenorii tripod by manual induction of the unreduced female gametes and the like in the conventional tripod cultivation method of siraitia grosvenorii are solved effectively, the tripod can be successfully obtained conveniently and easily in one growing period, and technical support is provided for the creation of abundant new siraitia grosvenorii tripod germplasms as well as the cultivation of new seedless tripod varieties.

This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.

A method for evaluating the quality of mammalian oocytes comprises determining the expression level of one or more of the genes ACPP, AQP11, CCDC126, CLU, CYP11 A1, CYP19A1, EGR3, FN1, FOSL2, GMNN, HRAS, HSD3B2, HS-D17B1, HSD11B2, HSDL1, IGF1, IGFBP4, IGFBP5, IRS1, KCNK3, KLF6, NEK6, SMAD7 or STC1 in a test sample derived from a cumulus cell or granulosa cell associated with the oocyte, and comparing the expression level of said at least one marker gene expression in the sample with the expression level in a control. Differential expression of the gene between the sample and the control is indicative of the quality of the oocyte.

The invention relates to an external Chinese medicinal preparation for preventing hair loss and promoting hair generation, which can inhibit hair loss and promote hair regeneration. The external Chinese medicinal preparation is prepared mainly by brewing the following Chinese medicinal components in alcohol: tuber fleeceflower root, Chinese angelica, safflower, lightyellow sophora root, fresh ginger and the like, is applied on scalp at a position of hair loss, increases the nutrition and activity of hair mother cells and recovers the physiological function of hair follicles by regulating the local microenvironment of the hair, and solves the problems of difficult hair regeneration and difficult long-term regeneration maintenance in alopecia areata, alopecia totalis and hair loss.

The invention relates to the technical field of maize gene engineering, in particular to application of a maize GRMZM2G417164 gene in adjustment of stomata development. The maize GRMZM2G417164, serving as a transcription factor, regulates some genes associated with the stomata development, and adjusts an associated process of maize stomata development. A series of experiments prove that a proteincoded by the GRMZM2G417164 gene controls transfer of a signal from a guard mother cell to a subsidiary mother cell and a final longitudinal division of the guard mother cell to form a guard cell. After the gene is mutant, a plant cannot form a normal guard cell and a normal subsidiary cell. Therefore, deep research and analysis on GRMZM2G417164 gene functions has an important theoretical significance and a practical application value for exploring a maize stomata development mechanism and improving maize resistance.

The invention provides a preparation method for an anti-oxidation anti-ultraviolet active yeast extract by using an atmospheric pressure low temperature plasma generating device, belonging to the field of application of low temperature plasma technology. The preparation method is characterized by comprising the following successive steps: culturing yeast cells; selecting a type of an atmospheric pressure low temperature plasma generating device and setting working parameters; treating a suspension of the yeast cells by using atmospheric pressure low temperature plasma; and extracting active substances of the yeast cells having undergone plasma treatment. The preparation method provided by the invention has the advantage of capacity of highly efficiently and conveniently changing the activity of an enzyme in the yeast cells. The prepared active yeast extract can be used as an additive for articles of everyday use and has anti-ultraviolet, anti-oxidation, anti-ageing, fresh-keeping, sterilization and anti-inflammation effects and the like. According to the invention, since the active yeast extract is produced by using microbial fermentation, the environment and natural resources pose no influence, industrialization and automation can be easily realized, and the method has the advantages of low production cost, easiness, stability, easy regulation and control and a high success rate. Enterprises of various scales can invest on the preparation method provided by the invention.