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369 results about "Mother cells" patented technology
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In cell division, a mother or parent cell is the cell that divides to give rise to two daughter cells. In mitosis, the two daughter cells contain the same genetic content as the mother cell. In meiosis, the daughter cells have different genetic content and half the number of chromosomes of the mother cell.
Imaging and evaluating embryos, oocytes, and stem cells
Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and / or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Method of cloning reproductive and respiratory syndrome resisting pig
Owner:CHINA AGRI UNIV
Generation of highly diverse library of expression vectors via homologous recombination in yeast
InactiveUS6410271B1High affinityEasy to assembleFusion with DNA-binding domainImmunoglobulinsDouble strandedYeast
Methods are provided for generating highly diverse libraries of expression vectors encoding fusion proteins such as single-chain antibodies via homologous recombination in yeast. The method comprises: transforming into yeast cells a linearized yeast expression vector having a 5'- and 3'-terminus sequence at the site of linearization and a library of insert nucleotide sequences that are linear and double-stranded; and having homologous recombination occur between the vector and the insert sequence such that the insert sequence is included in the vector in the transformed yeast cells. The insert sequence comprises a first nucleotide sequence encoding a first polypeptide subunit, a second nucleotide sequence encoding a second polypeptide subunit, a linker sequence encoding a linker peptide that links the first and second polypeptide subunits, and a 5'- and 3'-flanking sequence at the ends of the insert sequence which are sufficiently homologous to the 5'- and 3'-terminus sequences of the linearized yeast expression vector, respectively, to enable homologous recombination to occur. The first polypeptide subunit, the second polypeptide subunit, and the linker polypeptide are expressed as a single fusion protein; and the first and second nucleotide sequences each independently varies within the library of expression vectors.
Owner:GENETASTIX CORP
Overexpression porcine co-stimulatory 4-1BB vector and application thereof
InactiveCN105087620AHigh copy numberLower activation thresholdVector-based foreign material introductionAnimal husbandryInteinEmbryo
The invention provides an overexpression porcine co-stimulatory 4-1BB vector and application thereof. PCR (polymerase chain reaction) amplification is performed on a left homologous arm and a right homologous arm of an intron 1 of a rosa26 gene, a 4-1BB regulatory sequence and an OCT4 specific promoter; the left homologous arm, a 4-1BB expression cassette, LoxP locus-contained Cre and Neo expression cassettes, the right homologous arm and negative selection DTA diphtheria toxin are connected in sequence to obtain a 4-1BB homologous recombinant vector p4BOCNDR; the vector and a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR-associated) targeting vector of sgRNA (small guide ribonucleic acid) containing the intron 1 of the specific targeting porcine rosa26 gene are transferred together into a porcine fetus fibroblast; by taking a positive cell as a donor cell and an oocyte as a recipient cell, a cloned embryo is obtained through a somatic cell nuclear transfer technique; the cloned embryo is transplanted into a porcine uterus for fetation to obtain a transgenic pig integrating a 4-1BB gene at the fixed point of a first intron of the rosa26 gene and automatically deleting a marker gene.
Owner:CHINA AGRI UNIV
Nucleic acids encoding glial cell line-derived neurotrophic factor (GDNF)
ActiveUS7226758B1Prevent nerve damageOrganic active ingredientsNervous disorderGlial cell line-derived neurotrophic factorSerum free
A novel neurotrophic factor referred to as glial cell line-derived neurotrophic factor (GDNF) has been identified and isolated from serum free growth conditioned medium of B49 glioblastoma cells. Rat and human genes encoding GDNF have been cloned and sequenced. A gene encoding GDNF has been subcloned into a vector, and the vector has been used to transform a host cell in order to produce biologically active GDNF in a recombinant DNA process.
Owner:AMGEN INC
Method for constructing human neuroblastoma cell line of which CAPNS1 gene is knocked out based on CRISPR/Cas technology
InactiveCN108753772ALower levelGenetically modified cellsStable introduction of DNAGenetic engineeringEnzyme digestion
The invention relates to the technical field of genetic engineering and particularly relates to a method for constructing a human neuroblastoma cell line of which a CAPNS1 gene is knocked out based ona CRISPR / Cas technology. The invention provides the method for knocking out the CAPNS1 gene of human neuroblastoma cell. After plasmid transfection into SK-N-SH cells, a monoclonal antibody is prepared. After Cruiser<Tm> Enzyme digestion, the clone is positive clone. The monoclonal sequencing result shows that the CAPNS1 gene-free SK-N-SH cell line is successfully constructed. In the CAPNS1<- / ->group, the CAPNS1 protein and Calpain1 and Calpain2 protein levels are significantly reduced. The result shows that the CAPNS1 gene-free SK-N-SH cell line is successfully constructed.
Owner:NANHUA UNIV
Compositions and Methods for Treating Glioblastoma GBM
Methods of treating a malignant glioma in a subject are disclosed. The methods comprise administering to the subject a therapeutically effective amount of a viral vector comprising: (i) a first polynucleotide sequence encoding a Fas-chimera (Fas-c), said first polynucleotide sequence comprising SEQ ID NOs: 2 and 3; and (ii) a second polynucleotide sequence encoding an endothelial cell-specific promoter or a periendothelial cell-specific promoter.
Owner:VASCULAR BIOGENICS
Using Alternating Electric Fields to Increase Cell Membrane Permeability
ActiveUS20200009377A1Good cell membrane permeabilityReduced viabilityElectrotherapyEnergy modified materialsCancer cellCell membrane
Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).
Owner:NOVOCURE GMBH +1
Using Alternating Electric Fields to Increase Cell Membrane Permeability
PendingUS20200009376A1Good cell membrane permeabilityReduced viabilityElectrotherapyEnergy modified materialsCancer cellGlioblastoma
Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV +1
System and methods for automated vitrification of biological materials
ActiveUS20160029619A1Bioreactor/fermenter combinationsBiological substance pretreatmentsVitrificationEmbryo
The present invention relaters to a system and methods for automated vitrification of mammalian oocytes or embryos. The system and methods enable automated processing of oocytes or embryos in vitrification solutions; robotically moving vitrification devices that carry processed cells for freezing in liquid nitrogen; automated sealing of the frozen devices; and transferring the sealed devices to an automated storage system for long-term cryopreservation.
Owner:THE GOVERNINIG COUNCIL OF THE UNIV OF TORANTO
Methods for in vitro fertilization
InactiveUS6838235B2Improve developmentSpeed up the processNew breed animal cellsCulture processPlant Germ CellsReproductive tract
Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.
Owner:VITROLIFE AB
Preparation and application of ergosterin derivative
InactiveCN102070695AReplicative lifespan prolongation activitySimple structureOrganic active ingredientsAntinoxious agentsSporeLead compound
The invention provides an ergosterin derivative which is obtained by extraction, separation and purification from traditional Chinese medicine ganoderma lucidum spore powder. In the invention, an anti-aging yeast model, namely a K6001 yeast cell and a mutant strain thereof (delta uth1 and delta skn7) prove that the ergosterin derivative influences the gene expression of the UTH1 by regulating the activity of the Skn7 so as to prolong the duplicability life of yeast. The compound is used as a lead compound to optimize the structure, and can be used for preparing medicaments for delaying aging and preventing or / and treating aging diseases. The general structure formula of the ergosterin derivative is as shown in the specification.
Owner:ZHEJIANG UNIV
Apparatus and methods for culturing and/or transporting cellular structures
InactiveUS20100196871A1Easy accessBioreactor/fermenter combinationsAnimal reproductionDiffusionLiquid medium
An apparatus for culturing and / or transporting embryos, oocytes or other cellular structures, comprising a housing (12) having a gas space (28) and a media space (18) for a liquid medium separated from one another by a barrier (16) having one or more gas permeable regions to allow gas diffusion from the gas space to the media space, and an essentially gas-tight gas closure means (14) adapted to restrict the passage of gas into the container from the exterior environment, and liquid closure means (14, 16) adapted to engage with the housing to form a liquid-tight seal for retaining liquid in the media space. The barrier preferably comprises an at least partly gas-permeable insert (16) that when inserted into the housing forms together with the housing a gas space (28) and a liquid media space (18) in gas communication with one another via diffusion through the insert. The insert may be at least partly porous, the pores of the insert comprising at least part of the gas space.
Owner:ROBIO SYST
Novel hepatocyte-like cells and hepatoblast-like cells derived from hBS cells
InactiveUS20080019950A1Improve predictabilityReduce needBiocideHepatocytesIn vitro studyMother cells
The present invention relates to a novel hepatocyte-like cell population derived from hBS cells and to the potential use of such heopatocyte-like cells in e.g. medical treatment, drug screening and toxicity testing. Furthermore, the invention relates to hepatoblast-like cells that may have suitable characteristics so that they can be used for the same applications as the hepatocyte-like cells and that furthermore may be used in in vitro studies of hepatogenesis such as early hepatogenesis or hepato-regenerative disorders. Both the hepatocyte-like and the hepatoblast-like cells according to the invention express drug transporter and / or drug metabolising characteristics either at the gene or protein expression level.
Owner:CELLARTIS AB (SE)
Multi-copy high expressed recombined plectasin by pichia pastoris
ActiveCN102409003AIncrease expression abundanceFungiAntibody mimetics/scaffoldsPichia pastorisPlectasin
The invention discloses a preparation method of multi-copy high expressed recombined plectasin by pichia pastoris. The method comprises the following steps: a plectasin expressing gene sequence is designed according to preference performance to codon translated by pichia pastoris; the optimized plectasin gene is fused on an alpha-factor signal peptide C terminus of an expression vector pPICZalphaA to construct a single-copy expression vector, the vector comprises a plectasin expression cassette containing a start signal element alcohol oxygen dehydrogenase strong promoter (AOX), alpha-factor signal peptide gene and a plectasin gene fused in C terminus, a stop signal element AOX (TT) and the like. A complementation principle of restriction endonuclease Bg1II and BamHI cohesive end is used to obtain plectasin gene-containing recombinant plasmid of different copy cascade expression cassettes, pichia pastoris is electrotransformed and secreted and expressed plectasin with high efficiency under the methanol induction. The expression level and plectasin gene copy number exist a linear relation. The constructed multi-copy high expressed yeast cells can be used for raising the output and reducing the cost, and is adapted to large scale production of plectasin.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Treating Tumors Using TTFields Combined with ABT-751
Viability of cancer cells (e.g., glioblastoma cells) can be reduced by administering ABT-751 to the cancer cells and applying an alternating electric field with a frequency between 100 and 400 kHz (e.g., 200 kHz) to the cancer cells. Notably, experiments show that the combination of ABT-751 and the alternating electric field produces synergistic results for certain glioblastoma cell lines.
Owner:NOVOCURE GMBH
Long-term in-vitro culture and directional differentiation system and method for liver stem cell
ActiveCN104388383AMicrobiological testing/measurementDigestive systemMolecular phenotypeDirected differentiation
The invention relates to the technical field of biomedical engineering and particularly relates to a long-term in-vitro culture and directional differentiation system and method for a liver stem cell. The long-term in-vitro culture and directional differentiation system comprises an amplification culture medium with specific chemical components, and a differential medium with specific chemical components, wherein the amplification culture medium is used for carrying out in-vitro culture of a mouse or human liver stem cell, and the differential medium is used for carrying out induced differentiation on the mouse or human liver stem cell to form a matured liver cell. By using the long-term in-vitro culture and directional differentiation system and method, a selectively-amplified liver stem cell in mother cells of the liver can be obtained from a mouse embryonic liver tissue or through human multipotential stem cell differentiation, the liver stem cell can be cultured for more than 20 generations under such a condition, and the stable molecular phenotype of the liver stem cell is maintained. By using the long-term in-vitro culture and directional differentiation system and method, the cultured mouse or human liver stem cell can be further subjected to induced differentiation to form the matured liver cell with functions of secreting albumin, metabolizing urea and the like.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Pluripotent mammalian cells
InactiveUS20050210537A1Enhance chromatin remodellingStably integratedNew breed animal cellsGenetic material ingredientsReprogrammingCell type specific
The invention relates to a method of making pluripotent stem cells that does not involve the formation of early preimplantation embryos or fetal tissue. The method has general utility in the production of pluripotent stem cells from many mammalian species but has particular application in man where pluripotent stem cell production can be customized to particular human individual. The method involves the fusion of donor somatic or stem cells (or their karyoplasts) with cytoplasmic, membrane-delimited fragments of mammalian oocytes or zygotes. After the initial genomic reprogramming occurs, the cells can proliferate and thus multiply in vitro yielding a large number of autologous cells for cell therapy application. The result of this process is a cell population genomically identical to the somatic, differentiated cells derived from an individual patient. However, these cells are pluripotent in that upon application of specific growth factors, the cells are capable of differentiating into specific cell types as required by the sought clinical indication.
Owner:DOMINKO TANJA +4
Human liver cancer high-transfer cell strain with stable expression of fluorescent protein and construction method thereof
InactiveCN101381706AAvoid pollutionImprove efficiencyVector-based foreign material introductionForeign genetic material cellsCarcinoma cell lineCancer cell
The invention belongs to the field of micro-organism animal cell line and relates to a human hepatoma cell line which can emit high-intensity red or green fluorescence and has high transferring ability of lung and lymph node metastasis, and a method for establishing the same. The method comprises the following steps: using the human hepatoma cell line HCCLM3 and HCCLM6 which have high transferring ability of the lung and lymph node metastasis as mother cells, performing cotransfection on plasmid DNA of 239 cells through slow virus packaging plasmids to obtain false slow virus particles by expressing red or green fluorescent protein genes through eucaryon, and infecting liver cancer cell strains of the mother cells to obtain the chromosome integrated hepatoma cell line which has high transferring ability of the lung and lymph node metastasis and can stably expressing the red or the green fluorescence. The human hepatoma cell line which has high transferring ability of the lung and lymph node metastasis in vitro can be applied to the tracer studies on tumor cells, the molecular mechanism studies on the recurrence and transferring of liver cancer, as well as the pre-clinical drug efficacy studies on new anti-tumor drugs, thus the human hepatoma cell line has wide application prospect.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV
Method for identifying modulators of NAv ion channels
InactiveUS7195879B2Compound screeningCell receptors/surface-antigens/surface-determinantsBlastomaIonic Channels
Owner:JANSSEN PHARMA NV
Human source Fab antibody for anti recombined alkalescent fibroblast growth factor and application
InactiveCN101092457AHigh affinityImprove featuresImmunoglobulins against growth factorsAntibody ingredientsSurface displayFibrosis
This invention discloses anti-recombinant alkaline fibroblast growth factor human-derived Fab antibody and its application. The antibody segment genome comprises: heavy chain Fd chain and light chain kappa chain. This invention utilizes phage surface display technique to successfully construct kappa chain and Fd chain genes of human-derived Fab antibody that can neutralize recombinant human bFGF or FGF-2. Functional neutralizing antibody that can specifically bind bFGF or FGF-2 is obtained in prokaryotic cells, yeast cells, insect cells and eukaryotic cells, which can be used to prepare antibody drugs that can diagnose and treat tumors, and inhibit kidney, liver and lung fibrosis. The bFGF or FGF-2 human-derived Fab antibody has high affinity and specificity, and can be directly used to develop antibody drugs.
Owner:JINAN UNIVERSITY
In-vitro maturation culture method for oocyte of mouse and method for establishing parthenogenetic embryonic stem cell line
InactiveCN101735978ASimple ingredientsLow costMicroorganism based processesEmbryonic cellsStem cell lineBiological activation
The invention discloses an in-vitro maturation culture method for an oocyte mouse and a method for establishing a parthenogenetic embryonic stem cell line. In the in-vitro maturation culture method, an immature oocyte is cultured in a basic culture solution in which the HCG (human chorionic gonadotropin) and PMSG (pregnant mare serum gonadotropin) are added, and the maturation rate of the oocyte can reach more than 83 percent. In the method for establishing a parthenogenetic embryonic stem cell line, the parthenogenetic activation culture development is carried out on the mouse oocyte after in-vitro maturation culture to obtain the embryonic stem cell line; a hepatocyte obtained by the method has no immunogenicity, is simultaneously equivalent to a stem cell derived from a fertilized embryo on totipotency and has guiding significance for utilizing in-vitro maturation of the immature oocyte of a human and separating parthenogenetic embryonic stem cells. Aiming at the current conditionsof human ovum donation shortage, great discarding of clinical immature oocytes by an assisted reproductive technology, and the like, the two methods are combined to lay the foundation for developing and utilizing the in-vitro maturation culture of the immature oocyte of people and further researching the parthenogenetic embryo by utilizing an in-vitro mature ovum and separating the embryonic stemcells.
Owner:SUN YAT SEN UNIV
Enzyme treatment of glucans
InactiveUS20050255565A1Increase mobilityEnhanced ability to stimulate immune systemFungiSugar derivativesBiotechnologyGlucanase
β-(1-6)-Glucanase treatment of glucan from yeast cells, pure or feed grade, especially yeast from the family Saccharomyces and particularly Saccharomyces cerevisiae, provides a novel glucan product suitable for use in enhancing the stimulation of host animal immune systems. Solubilization of such yeast cell glucan is further disclosed to extend the usefulness of yeast cell glucan as an adjuvant.
Owner:BIOTEC PHARMACON
Hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions and preparation method thereof
The embodiment of the invention provides a hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions and a preparation method thereof, and relates to the field ofcosmetics. The hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions has the functions of regulating the secretion of scalp grease, activating hair mother cells and promoting the division of the hair mother cells, transforms a hair follicle resting period into a growing period, makes the hair follicle primordium revived, and can effectively play roles inpreventing hair loss and making hair grow. The hair follicle primordial activating liquid with hair loss-preventing and hair-growing functions at least comprises the following components by the mass percentage: 0.01-1% of pantothenol, 0.01-5% of a plant extract, 0.01-1% of resveratrol and 0.01-1% of menthol.
Owner:陕西佰瑞衡健康科技有限公司
Yeast cell-based Ni-Co-S loaded porous carbon material as well as preparation method and application thereof
InactiveCN110942924AAvoid harmSolving Tolerance IssuesHybrid capacitor electrodesCarbon preparation/purificationPorous carbonCarbonization
The invention discloses a Ni-Co-S loaded porous carbon material based on yeast cells. The preparation method comprises the following steps: preparing carbonized yeast cell porous carbon, carrying outhydrothermal reaction on the carbonized yeast cell porous carbon, cobalt nitrate, nickel nitrate and urea, and oxidizing and vulcanizing to obtain the material, the obtained material is of a core-shell structure, and cobalt and nickel elements are uniformly loaded on the surface of the yeast cell porous carbon in the form of NiCo2S4; wherein the shell-core structure is formed by carbonizing ellipsoidal single-cell fungal yeast, and carbonized yeast cells are of a hollow carbon sphere structure. The preparation method comprises the following steps: 1) preparing yeast cell porous carbon; 2) adsorbing cobalt-nickel metal ions by yeast cell porous carbon; and 3) vulcanizing the yeast cell porous carbon adsorbing the cobalt-nickel metal ions. When the material is used as a supercapacitor, the material is charged and discharged in a range of-0.2 to 0.5, and when the discharge current density is 1 A / g, the specific capacitance is 700 to 800 F / g. The invention has the following advantages: noharm is caused to human body; and the tolerance problem of the yeast cell metal salt solution is solved.
Owner:GUILIN UNIV OF ELECTRONIC TECH
Screening method for roes of fishes in Acipenseridae
InactiveCN102440202AAvoid damageImprove the quality of fertilized eggsClimate change adaptationPisciculture and aquariaVitellogeninsGlutamic-Oxaloacetic Transaminase
The invention relates to a screening method for roes of fishes in Acipenseridae, which aims to solve the problem that the evaluating method is mistaken as the detecting method for the quality of the fish roes is lack of overall evaluation. The evaluation method is as follows, firstly, taking out a roe from the fish in Acipenseridae; secondly, preparing roe extracting solution; thirdly, preparing blood serum; fourthly, preparing body fluid; fifth, determining the polarization indexes of mother cells of the roe; sixthly, determining the density of blood serum vitellogenin; seventhly, determining the contents of Fe ions, roe Zn ions, roe VE and roe glutamic-oxaloacetic transaminase; eighthly, determining the contents of ACP and AKP; and ninthly, if the determined indexes simultaneously meet the target values, the remaining roes in the same ovary are mature, and then the screening of the roes of the fishes in Acipenseridae is accomplished. By adopting the invention, the fertilization rate of the sturgeon, the hatchability of the sturgeon and the survival rate of the fry are more than 85%, 95% and 90% respectively. The screening method is used for breeding the fishes in Acipenseridae.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Method or Use of a Solubilized Glucan Product to Increase Immunostimulation in Animals
InactiveUS20080124349A1Increase mobilityEnhanced ability to stimulate immune systemOrganic active ingredientsBiocideBiotechnologyAdjuvant
Owner:BIOTEC PHARMACON
Plant growth conditioner for reducing damage caused by high temperature to rice, and using method and application of plant growth conditioner
ActiveCN104054706AThe effect of preventing heat damageIncrease photosynthetic rateBiocidePlant growth regulatorsGibberellic acidGrowth plant
The invention discloses a plant growth conditioner for reducing damage caused by high temperature to rice, and a using method and application of the plant growth conditioner. The conditioner comprises gibberellin and salicylic acid, wherein the weight ratio of gibberellin to salicylic acid is (0.5-1.5):(0.15-0.6). The conditioner can also comprise chitosan oligosaccharide according to a certain ratio. When the conditioner is used, an aqueous solution form is adopted, wherein an aqueous solution is prepared from 50 to 150mg / L of gibberellin, 15 to 60mg / L of salicylic acid and 50 to 200mg / L of chitosan oligosaccharide, and is applied to leaves in a spraying way during the meiosis stage of pollen mother cells of rice, and 30 to 45 liters of aqueous solution is applied to each acre. The plant growth conditioner can be applied to reduce the damage caused by high temperature to rice, and is suitable for popularization and application in a rice culturing region on the middle and lower reaches of Changjiang River, particularly in a region at which the temperature in a flowering period is always high, and high and stable yield of rice is ensured.
Owner:CHINA NAT RICE RES INST
Targeted Osmotic Lysis of Cancer Cells
ActiveUS20130184218A1Effective treatmentHigh expressionBiocideElectrotherapyNon cancerSmall-cell carcinoma
A targeted osmotic lysis (TOL) of tumor cells that over-express voltage-gated sodium channels (VGSCs) has been developed that uses a combined therapy of a drug that blocks sodium, potassium-adenosine triphosphatase (Na+, K+-ATPase) that is then followed by an activation of VGSCs, for example, by electrical or pharmacological stimulation. Activation of VGSCs conducts sodium into the cancer cells in much greater amounts than non-cancer cells. Water follows this sodium gradient into the cancer cells, causing swelling and lysis. Because non-cancerous cells do not over-express VGSCs, less sodium and less water will enter the cells, and the non-cancerous cells will not lyse. This method is applicable to all cells that over-express VGSCs, including, but not limited to, highly invasive breast cancer, prostate cancer, small cell lung cancer, non-small cell lung carcinoma, lymphoma, mesothelioma, neuroblastoma, and cervical cancer.
Owner:BOARD OF SUPERVISORS OF LOUISIANA STATE UNIV & AGRI & MECHANICAL COLLEGE
Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells
The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention. The invention further relates to a population of human hepatoblasts-like cells expressing HNF4α and expressing substantially AFP for use in a method of treatment of the human body.
Owner:UNIV DE NANTES +1
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