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Screening method for roes of fishes in Acipenseridae

A screening method and fish technology, which is applied in the field of screening eggs of sturgeons, can solve the problems of lack of comprehensive evaluation and deviation of evaluation methods, and achieve the effects of high accuracy, high sensitivity, and small damage

Inactive Publication Date: 2012-05-09
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of lack of comprehensive evaluation in the existing methods for detecting the quality of fish eggs, which leads to deviations in the evaluation method, and provides a screening method for eggs of sturgeons

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Specific embodiment one: the screening method for the ovum of sturgeon family fish in this embodiment is carried out according to the following steps: one, take out the ovum from the body of sturgeon family fish; Fresh weight 5g, add 12-17mL Tris-HCl buffer solution with a concentration of 0.02-0.05mol / L and a pH of 7.2-8.0, grind at 0-4°C, and centrifuge the obtained homogenate at a speed of 12000-20000r / min for 10 ~20min, take the supernatant, which is the egg extract; 3. Serum preparation: put 20~30mL of fresh sturgeon tail vertebrae venous blood or tail vertebrae arterial blood at room temperature for 20~40min, then in 0~4 Store at low temperature for 20 to 25 hours, then centrifuge at 2000 to 4000 r / min for 20 to 30 minutes, extract serum, and store it for later use; 4. Preparation of body fluid: Filter the eggs taken out in step 1 through a sieve, and collect 15 mL of egg cavity fluid per tail , stored in a liquid nitrogen tank: 5. Determination of the polarizatio...

specific Embodiment approach 2

[0013] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the method for taking out the ovum from the sturgeon body in step one is: anesthetize the sturgeon with clove oil aqueous solution, then 3-5 ovum on the ventral surface of the tail At the bone plate, use an ovipositor to go straight into the body cavity along the abdominal wall muscles to take out the eggs; the clove oil aqueous solution is composed of clove oil and water in a volume ratio of 1:10 4 composition. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Specific embodiment three: what this embodiment is different from specific embodiment one or two is: the method for measuring the polarization index of oocyte in step five is: get 30 ovums that step one obtains, put into boiling water, boil 2 After ~4 minutes, cool to room temperature, then cut along the polar axis of the egg, measure the distance from the blastocyst to the animal polar egg membrane and the distance from the blastocyst to the plant polar egg membrane, and calculate the polarization index of the oocyte according to the following formula, Polarization index of oocyte = distance from blastocyst to animal polar egg membrane / distance from blastocyst to plant polar egg membrane. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a screening method for roes of fishes in Acipenseridae, which aims to solve the problem that the evaluating method is mistaken as the detecting method for the quality of the fish roes is lack of overall evaluation. The evaluation method is as follows, firstly, taking out a roe from the fish in Acipenseridae; secondly, preparing roe extracting solution; thirdly, preparing blood serum; fourthly, preparing body fluid; fifth, determining the polarization indexes of mother cells of the roe; sixthly, determining the density of blood serum vitellogenin; seventhly, determining the contents of Fe ions, roe Zn ions, roe VE and roe glutamic-oxaloacetic transaminase; eighthly, determining the contents of ACP and AKP; and ninthly, if the determined indexes simultaneously meet the target values, the remaining roes in the same ovary are mature, and then the screening of the roes of the fishes in Acipenseridae is accomplished. By adopting the invention, the fertilization rate of the sturgeon, the hatchability of the sturgeon and the survival rate of the fry are more than 85%, 95% and 90% respectively. The screening method is used for breeding the fishes in Acipenseridae.

Description

technical field [0001] The invention relates to a method for screening ova of sturgeons. Background technique [0002] Sturgeon (Acipenseriformes) is a group of ancient creatures with high research value and economic value. my country began research on sturgeon farming in the early 1990s, and the scale has grown rapidly. By 2008, my country had become the world's largest sturgeon breeding country, with an annual output of 21.396 tons, accounting for 83% of the world's total sturgeon. The egg quality of sturgeon is directly related to embryo development and the survival and growth of larvae, and is closely related to the breeding effect. In addition, the quality of gametes can also feed back the breeding status of broodstock, and is also of great significance in guiding the production of seedlings. [0003] The evaluation of fish egg quality is usually the detection of its external morphological and structural characteristics and production performance, such as color, ferti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张颖曲秋芝孙大江刘晓勇
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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