4 results about "Vitellogenin" patented technology

A liver-specific expressional regulatory element, including, in order from 51 to 31: a promoter of a dog serpinAl gene or a functional fragment thereof, a promoter of a Xenopus laevis vitellogenin A2 gene or a functional fragment thereof, a promoter of a Xenopus laevis albumin gene or a functional fragment thereof, and a promoter of a human serpinAl gene or a functional fragment thereof.

The invention belongs to the technical field of biology, and particularly relates to cherax quadricarinatus oocyte targeting peptide VNP as well as a design and screening method and application thereof. The invention discloses a cherax quadricarinatus oocyte targeting peptide VNP design and screening method. The method comprises the following steps: (1) predicting an interaction site between cherax quadricarinatus vitellogenin and a vitellogenin receptor by using AlphaFold3; (2) intercepting an oligopeptide sequence according to the principle that the number of hydrogen bonds and salt bridges interacting in continuous 20 amino acids is greater than 4; and (3) screening the cherax quadricarinatus oocyte targeting peptide according to the binding rate constant and the dissociation rate constant of the oligopeptide and the vitellogenin receptor by utilizing a biological membrane interference technology. The breakthrough lays a technical foundation for establishing a gene editing element delivery method with oocyte targeting, and is expected to be applied to the field of cherax quadricarinatus gene editing.

The application belongs to the technical field of biomolecular markers, and particularly relates to a primer set for detecting a SNP molecular marker significantly related to the fecundity trait of Procambarus clarkii and application thereof. The primer set can quickly and effectively realize accurate genotyping of a SNP site of a vitellogenin Vg gene of the Procambarus clarkii, and the detection can be completed only by using conventional PCR and agarose gel electrophoresis, so that the detection process is simple, time-consuming is short, and cost is low, and the primer set is suitable for large-scale and high-throughput parent screening. The marker is significantly related to the fecundity traits such as clutch size of the Procambarus clarkii, the genotyping result is stable and reliable and has good repeatability, only the end of the tail fan needs to be sampled, dissection is not needed, and the genotype identification can be completed in the early stage of breeding, so that the selection and breeding cycle is greatly shortened, the high-fecundity parent screening efficiency is improved, the problems such as long selection and breeding cycle and low efficiency in traditional phenotype selection and breeding are effectively solved, a stable and practical molecular tool is provided for the genetic improvement and efficient breeding of the Procambarus clarkii, and the application has important industrial application value.

The invention belongs to the technical field of biomolecular markers, and particularly relates to a primer group for detecting an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to the reproductive capacity character of cherax quadricarinatus and application of the primer group. By means of the primer group, accurate typing of the cherax quadricarinatus vitellogenin Vg gene SNP loci can be rapidly and effectively achieved, detection can be completed only through conventional PCR and agarose gel electrophoresis, the detection process is simple and convenient, consumed time is short, cost is low, and the primer group is suitable for large-scale and high-throughput parent screening. The marker is obviously associated with reproductive characters such as egg carrying amount of cherax quadricarinatus, the typing result is stable and reliable, the repeatability is good, only tail fan tail end sampling is needed, dissection is not needed, genotype identification can be completed in the early stage of breeding, the breeding period is greatly shortened, the high-reproductive-capacity parent screening efficiency is improved, and the breeding cost is reduced. The method effectively solves the problems of long period, low efficiency and the like of traditional phenotype breeding, provides a stable and practical molecular tool for germplasm improvement and efficient breeding of cherax quadricarinatus, and has important industrial application value.