75 results about "Vitellogenin" patented technology

The present invention is directed to a simple method for absolute quantification of plasma vitellogenin from two or more different fish species such as Rainbow trout and Atlantic salmon, or Atlantic cod and haddock. In the case of Rainbow trout and Atlantic salmon, plasma samples obtained from control and β-estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyzed by high performance liquid chromatography coupled to an electrospray quadrupole-time-of-flight tandem mass spectrometer, using a deuterated homologue peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a ‘pseudo’ selected reaction monitoring mode by which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ˜5%) and sensitivity (limit of quantification of 0.009 mg / mL) achieved by this simple assay allow it to be considered as an alternative to immunological assays. In the case of Atlantic cod and haddock, the amino acid sequence of the vitellogenin protein has not yet been determined, but, the Atlantic cod vitellogenin has been characterized using a ‘bottom-up’ mass spectrometric approach. Vitellogenin synthesis was induced ‘in vivo’ with β-Estradiol, and subjected to trypsin digestion for characterization by matrix-assisted laser desorption / ionization-Quadrupole-Time-of-flight tandem mass spectrometry. A peptide mass fingerprint was obtained and ‘de novo’ sequencing of the most abundant tryptic peptides was performed by low energy collision induced dissociation-tandem mass spectrometry. Thus, the sequences of various tryptic peptides have been elucidated. It has also been determined that Atlantic cod vitellogenin shares a series of common peptides with the two different known vitellogenin sequences of Haddock, a closely related species. There are also disclosed novel isolated signature peptides, namely Thr-Tyr-Phe-Ala-Gly-Ala-Ala-Ala-Asp-Val-Leu-Glu-Val-Gly-Val-Arg, Asp Leu Gly Leu Ala Tyr Thr Glu Lys, Phe Phe Gly Gln Glu Ile Ala Asn Ile Asp Lys, Glu Ile Val Leu Leu Gly Tyr Gly Thr Met Ile Ser Lys and Tyr Glu Ser Phe Ala Val Ala Arg.

The invention discloses an enzyme-linked immunosorbent kit for detecting the vitellogenin of goldfish, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the vitellogenin of the goldfish is prepared; secondly, the polyclonal antibody of rabbit anti-vitellogenin of the goldfish is prepared; thirdly, the polyclonal antibody of the rat anti-vitellogenin of the goldfish is prepared; and fourthly, respectively one bottle of the pure product of the vitellogenin of the goldfish, the polyclonal antibody of the rabbit anti-vitellogenin of the goldfish and the polyclonal antibody of the rat anti-vitellogenin of the goldfish, one block of blank 96-hole enzyme-linked immunosorbent assay board and respectively one bottle of the serum of a negative contrasting group, coating liquid, confining liquid, sample diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by horse radish peroxidase are filled into a box body together, thus obtaining the enzyme-linked immunosorbent kit for detecting the vitellogenin of the goldfish. The kit of the invention can be applied to the survey that the internal secretion disturbs chemical substances and can quantitatively detect the vitellogenin in the blood of the goldfish, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.

The invention relates to a test kit for qualitatively detecting fish vitellogenin by utilizing lipovitellin antibody. The test kit comprises pure product of goldfish lipovitellin and anti-rat goldfish lipovitellin polyclonal antibody. The preparation method of the test kit comprises the following steps: obtaining goldfish lipovitellin through purifying at first; then preparing anti-rat goldfish lipovitellin polyclonal antiserum through immune rats; and further purifying anti-rat goldfish lipovitellin polyclonal antibody. According to the invention, the test kit can be used for screening environmental estrogen materials and monitoring the situation of water environment which is polluted by the environmental estrogen materials, so that vitellogenin in fish blood, overall uniform serous fluid, skin mucus, liver tissues and hepatic cell nutrient solution can be monitored sensitively and conveniently. The test kit can be used for qualitatively detecting cyprinidae fish vitellogenin, and has the same monitoring sensitivity to the fish vitellogenin of a far provenance relationship fish. The biggest advantage of the test kit is that the test kit has a very wide application scope, and the minimum monitoring limit can reach 100ng / ml.

The invention discloses a kit for detecting that the internal secretion of the sea disturbs chemical substances, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the vitellogenin of snapper is prepared; secondly, the polyclonal antibody of rabbit anti-vitellogenin of snapper is prepared; and thirdly, one bottle of pure vitellogenin of the snapper, one bottle of the polyclonal antibody of the rabbit anti-vitellogenin of the snapper, one block of blank 96-hole enzyme-linked immunosorbent assay board and respectively one bottle of the serum of a negative contrasting group, coating liquid, confining liquid, sample diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by horse radish peroxidase are filled into a box body together, thus obtaining the kit for detecting that the internal secretion of the sea disturbs the chemical substances. The kit for detecting that the internal secretion of the sea disturbs the chemical substances can be applied to the detection that the internal secretion disturbs the chemical substances and can quantitatively detect the vitellogenin in the blood of the snapper, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.

The invention discloses a method for detecting the egg laying amount of insects by a vitellogenin gene, which provides application of a substance which is used for detecting the expression quantity of vitellogenin encoding genes of insect populations eating different substances to detection of the egg laying amount of insect populations eating different substances, wherein the amino acid sequence of vitellogenin is a sequence 2 in the sequence list. Experiments prove that the detection method provided by the invention can be used for detecting the egg laying amount of the insects and particularly the egg laying amount of arma chinensis with different nutrition sources, and the egg laying amount of the insects can be detected within only 2 to 3 days by the detection method while the detection requires 30 to 60 days by a traditional biological method. The detection method provided by the invention has the advantages that materials have wide sources and are easily available, and the operation is simple as well as time-saving and labor-saving, thus being suitable for popularization and application in insect commodity inspection and detection.

The invention relates to breeding for brown planthopper population based on male sterilty after RNAi and a control effect evaluation method. According to the invention, a RNAi breeding method is employed for silencing brown planthopper male testis growth gene PHF7, by detecting the mortality and gene silencing rate, the concentration of dsNlPHF7 is determined; the silenced male insect growth and reproduction parameter indexes are detected, which mainly comprise body weight, life span, accessory gland protein content and arginine content; the copulated male insect growth and reproduction parameter indexes are detected, which mainly comprise body weight, the life span, vitellogenin content, oviposition amount, and YLS richness; and the expression level of the vitellogenin gene in the copulated male insect fat is detected. The brown planthopper population increasing and controlling effects enable quantification evaluation by detecting the important reproduction parameter indexes of the brown planthopper population male insects and female insects, the method has the advantages of simple and fast operation, and has wide applicability, the method provides a novel control approach for preventing and treating brown planthopper, and has theory meaning and utility values.

The invention discloses a method for producing a recombinant rex rabbit nutrient dried meat slice. The method comprises the following steps of: (1) cleaning and picking; (2) smashing; (3) burdening; (4) pickling; (5) pushing on a disk; (6) drying; (7) baking; (8) flattening; and (9) cooling and chopping. According toIn the method, a plurality of nutrient substances such as transglutaminase, soybean protein isolate, ovalbumin, vitellogenin, whey protein, and casein and the like are added into rex rabbit meat, and an organic whole is formed by recombining with rex rabbit meat, so that the original nutrient characteristic of the rex rabbit meat is kept; and a plurality of nutrient elements needed by human bodies are added, so that the nutrient value of the recombinant rex rabbit dried meat slice is raised. The recombinant rex rabbit nutrient dried meat slice produced with the method has particular taste, fragrance, color and luster, good mouthfeel and long shelf life. According toIn the method, the rex rabbit meat of which skin is utilized is taken as a raw material, so that the production cost is lower, and the rex rabbit meat of which the skin is utilized is well utilized comprehensively.

On a surface of a well previously disposed on a plate, a primary antibody that recognizes vitellogenin is solid-phased, in the well a sample obtained from a test body exposed to an environment is injected to react, followed by injecting a secondary antibody that is labeled with an enzyme and recognizes the vitellogenin, further followed by injecting a chromogenic reagent to cause a coloring reaction and by measuring the stained amount, still further followed by calculating an amount of vitellogenin from the stained amount to evaluate an environment based on the amount of vitellogenin.

The present invention relates to a microstructure slicing technology, and specifically relates to an acipenser dabryanus oosperm tissue slicing method. The acipenser dabryanus oosperm tissue slicing method mainly comprises seven steps of material fixation, dehydration, transparentizing, wax dipping, embedding, slicing and staining; a fixing liquid used in the material fixation is a Bouin's fixing liquid and a formaldehyde fixing liquid with the mass fraction of 10%; and the total dehydration time is 10-20h. The acipenser dabryanus oosperm tissue slicing method aims at sturgeon oosperm which belongs to a viscid egg, has the characteristics of being rich in vitellogenin, tough and dense in egg envelope, narrow in perivitelline space, and the like, a complete serial tissue slicing method suitable for sturgeon oosperm is established, the sturgeon oosperm can be efficiently processed, and better paraffin sections can be made.