8 results about "Vitellogenin" patented technology

A liver-specific expressional regulatory element, including, in order from 51 to 31: a promoter of a dog serpinAl gene or a functional fragment thereof, a promoter of a Xenopus laevis vitellogenin A2 gene or a functional fragment thereof, a promoter of a Xenopus laevis albumin gene or a functional fragment thereof, and a promoter of a human serpinAl gene or a functional fragment thereof.

The invention discloses an application of a vitellogenin gene in regulating and controlling lipid metabolism and crawling behaviors of bees. The invention relates to a novel technology for regulating and controlling lipid metabolism and crawling behaviors of bees by knocking down vitellogenin gene expression, in particular to a method for inhibiting vitellogenin (Vg) gene expression by microinjection of mixed siRNA of three specific sequences into the brains of bees. Therefore, the possible effect of the compound in regulation and control of lipid metabolism and crawling behaviors of bees is explored. Results show that Vg gene expression of bee brains is significantly inhibited after microinjection of mixed siRNA, lipid metabolism of bees is significantly regulated and controlled, and crawling behaviors of bees are inhibited. Meanwhile, the behavior regulation and control technology involved in the invention is friendly to bees.

The present invention discloses the application of the vitellogenin gene in regulating lipid metabolism and crawling behavior of bees. The present invention is a new technology for regulating lipid metabolism and crawling behavior of bees by knocking down the expression of the vitellogenin gene. Specifically, a mixture of siRNAs with three specific sequences is microinjected into the bee brain to inhibit the expression of the vitellogenin (Vg) gene. Thereby, to explore its possible role in regulating lipid metabolism and crawling behavior of bees. The results show that after microinjecting the mixed siRNAs, the expression of the Vg gene in the bee brain is significantly inhibited, the lipid metabolism of bees is significantly regulated, and the crawling behavior of bees is inhibited. At the same time, the behavior regulation technology involved in the present invention is friendly to bees.

The invention belongs to the technical field of biology, and particularly relates to cherax quadricarinatus oocyte targeting peptide VNP as well as a design and screening method and application thereof. The invention discloses a cherax quadricarinatus oocyte targeting peptide VNP design and screening method. The method comprises the following steps: (1) predicting an interaction site between cherax quadricarinatus vitellogenin and a vitellogenin receptor by using AlphaFold3; (2) intercepting an oligopeptide sequence according to the principle that the number of hydrogen bonds and salt bridges interacting in continuous 20 amino acids is greater than 4; and (3) screening the cherax quadricarinatus oocyte targeting peptide according to the binding rate constant and the dissociation rate constant of the oligopeptide and the vitellogenin receptor by utilizing a biological membrane interference technology. The breakthrough lays a technical foundation for establishing a gene editing element delivery method with oocyte targeting, and is expected to be applied to the field of cherax quadricarinatus gene editing.

The present invention provides the use of human chorionic gonadotropin (HCG) in the preparation of a drug for repairing ovarian damage in tilapia, belonging to the field of aquaculture technology. The present invention provides the use of human chorionic gonadotropin in the preparation of a drug for repairing ovarian damage in tilapia. Human chorionic gonadotropin can increase the content of hormones and vitellogenin in serum and increase the expression of sex hormone-related genes in the ovary. At the same time, one or more of estradiol, human chorionic gonadotropin, luteinizing hormone-releasing hormone and coumestrol can increase the body weight of tilapia exposed to copper and cadmium contaminated water phase, providing a new idea for the preparation of new growth-promoting drugs.

The application belongs to the technical field of biomolecular markers, and particularly relates to a primer set for detecting a SNP molecular marker significantly related to the fecundity trait of Procambarus clarkii and application thereof. The primer set can quickly and effectively realize accurate genotyping of a SNP site of a vitellogenin Vg gene of the Procambarus clarkii, and the detection can be completed only by using conventional PCR and agarose gel electrophoresis, so that the detection process is simple, time-consuming is short, and cost is low, and the primer set is suitable for large-scale and high-throughput parent screening. The marker is significantly related to the fecundity traits such as clutch size of the Procambarus clarkii, the genotyping result is stable and reliable and has good repeatability, only the end of the tail fan needs to be sampled, dissection is not needed, and the genotype identification can be completed in the early stage of breeding, so that the selection and breeding cycle is greatly shortened, the high-fecundity parent screening efficiency is improved, the problems such as long selection and breeding cycle and low efficiency in traditional phenotype selection and breeding are effectively solved, a stable and practical molecular tool is provided for the genetic improvement and efficient breeding of the Procambarus clarkii, and the application has important industrial application value.

The invention discloses a method for detecting a reproductive toxicity marker by taking zebra fish as a model organism, which comprises the following two steps of: measuring the content of ovuprogen (VTG) in blood plasma of the zebra fish and preparing and analyzing gonad slices of the zebra fish. The endocrine disruption effect of CYF is evaluated by taking fish body sex hormone and VTG levels and gamete conditions in gonads as indexes, the increase of vitellogenin detected by the method indicates that the content of estrogen in male zebra fish is increased, the estrogen-like effect of CYF is verified, the male zebra fish also has VTG genes, and the CYF has a good endocrine disruption effect due to the stimulation of estrogen or estrogen-like effect. In the prior art, the VTG in the male fish body can rise, mature sperms and sperm cells are exposed by CYF, the two cells locally change, the sperm concentration degree is reduced, and sperm arrangement in some areas is sparse compared with that in a control group, so that the detection of the change of the VTG in the male fish body can be used as an effective means for evaluating whether a compound has an estrogen effect or not.

The invention belongs to the technical field of biomolecular markers, and particularly relates to a primer group for detecting an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to the reproductive capacity character of cherax quadricarinatus and application of the primer group. By means of the primer group, accurate typing of the cherax quadricarinatus vitellogenin Vg gene SNP loci can be rapidly and effectively achieved, detection can be completed only through conventional PCR and agarose gel electrophoresis, the detection process is simple and convenient, consumed time is short, cost is low, and the primer group is suitable for large-scale and high-throughput parent screening. The marker is obviously associated with reproductive characters such as egg carrying amount of cherax quadricarinatus, the typing result is stable and reliable, the repeatability is good, only tail fan tail end sampling is needed, dissection is not needed, genotype identification can be completed in the early stage of breeding, the breeding period is greatly shortened, the high-reproductive-capacity parent screening efficiency is improved, and the breeding cost is reduced. The method effectively solves the problems of long period, low efficiency and the like of traditional phenotype breeding, provides a stable and practical molecular tool for germplasm improvement and efficient breeding of cherax quadricarinatus, and has important industrial application value.