510 results about "Sturgeon" patented technology

Synchronous induced spawning for parent fish better solves the problem of sexual maturity through synchronous development of female and male parent fish. The invention discloses an artificial propagation method for sturgeon, which comprises the steps of selection of parent fish, parent fish rearing, induced spawning, artificial insemination, zygote hatching, artificial selection of fish fry, and fish fry rearing. In the artificial propagation method, by artificially reducing water temperature and ambient environment temperature, micro water flow is formed to simulate the ecological environment for the reproduction of sturgeon, the simulated ecological environment is similar to the conditions necessary for the reproduction of wild sturgeon in the natural world, and the artificial low temperature factor lasts for a long time, so as to always guarantee the low temperature environment for the maturity and awaiting delivery of parent fish of the sturgeon, and meet other condition requirements, so that the parent fish can rut and spawn all year round to finally achieve the factory production of the sturgeon fry; meanwhile, in the artificial propagation method, female parent fish and male parent fish of the sturgeon are separately put into an artificially simulated ecological environment for inducing oestrus, and then the degree of sexual maturity of the parent fish is identified to select the parent fish with basically developed sexual maturity.

The invention provides an antioxidation feedstuff for improving the immunity of sturgeons, which relates to an antioxidation feedstuff. The antioxidation feedstuff solves the problems that the sturgeons bred manually with the prior feedstuff have low immunity, are easy to be infected, are hard to cure with common drugs after catching a disease, and are sensitive to the common drugs. The antioxidation feedstuff for improving the immunity of the sturgeons is prepared by mixing fish meal, bean pulp, wheat middling, fish oil, soybean lecithin, aloe powder, cellulose, zeolite and a premix. The antioxidation feedstuff for improving the immunity of the sturgeons does not have obvious influence on the production performance of the sturgeons, obviously improves the blood plasma superoxide dismutase activity (P is less than 0.05), obviously reduces the blood plasma malonaldehyde activity (P is less than 0.05), improves the immunity of the sturgeons, and preventing the sturgeons from being infected and catching diseases.

The invention provides a feedstuff for sturgeon and hucho taimen at mouth - open period, which solves the problems of the conventional feedstuff that the growth rates of the sturgeon and hucho taimen are slow and the survival rates are low. The feedstuff comprises: dextrin, full cream milk powder, blood powder, white fish meal, soy protein, L-alanyl-L-glutamine, methylol cellulose and ore-mixing agent. When the feedstuff is used the for sturgeon and hucho taimen at mouth - open period, the growth rates and the survival rates of the sturgeon and hucho taimen are increased and the special growth rates of the sturgeon and hucho taimen are 1.5%-6.2% and the survival rates are more than 98%.

The invention discloses a sturgeon skin chrome tanning leather production process, which comprises the following steps: immersing in water; removing meat manually; degreasing; scraping oil slick; liming; scraping oil slick; liming again; scraping oil slick; deliming and softening; bleaching by oxidization; pickling; bleaching by reduction; chrome tanning; dyeing and greasing; re-tanning; washing; hanging and drying; and coating. In the invention, when the sturgeon skin chrome tanning leather production is adopted, the grease content in the sturgeon skin can be reduced obviously and the color of particle surface of the back of the sturgeon skin can be lightened; and thus, dyeing or production of light leather can be facilitated, the finished leather retains the natural attractive scales and special textures of sturgeon skin, the utilization value of the sturgeon skin is improved greatly, and the added value of the product is increased obviously.

The invention relates to food processing technology, in particular to technology for processing sturgeon caviar, which comprises steps of: fish selection for standby, caviar preparation and packagingby canning, wherein the caviar preparation process comprises strict processing processes such as opening belly and taking ovary, preserving and reducing temperature with broken ice, scrubbing, weighing and salting, leaching water and the like. The invention provides a scientific processing means for selecting fish and preparing roe, in particular the technology for processing the caviar which cankeep pure taste of the caviar and is aseptic, safe and reliable.

The invention provides a molecular marker for identifying amur sturgeon germplasm and a method for identifying amur sturgeon germplasm by virtue of the molecular marker. Amur sturgeon or a finished product thereof can be directly identified through sequence alignment on the basis of an interspecific sequence difference of an fzd8 gene segment obtained from PCR amplification of a specific primer between the amur sturgeon and other sturgeons. The molecular marker disclosed by the invention, which is used for analyzing and identifying an SNP site on the fzd8 nuclear gene segment screened from acipenseridae fishes, has the advantages of being accurate in experiment, reliable and rapid in identification, convenient and simple in operation, and the like.

The invention discloses a sturgeon sexuality difference molecular marker and an application thereof; the marker has the nucleotide sequence shown in SEQ ID NO.1; a DNA sequence shown in the SEQ ID NO.1 is detected by PCR primers, and according to a PCR detection result, the sturgeon sexuality is identified; a forward primer F of the PCR primers is 5'-CACACTGATGCTCTACATGT-3', a reverse primer R is 5'-AGGCTGGACAGTGATGCTGT-3', and the forward primer F and the reverse primer R are respectively shown in SEQ ID NO.2 and SEQ ID NO.3. The invention provides a nucleic acid fragment for detecting the sturgeon sexuality and the PCR primers for detecting the molecular marker related to sturgeon; the molecular marker is not limited by the age of the sturgeon, is accurate and reliable, is easy to operate, and has important application value in production.

The invention provides a wild training method and a wild training device for improving swimming capacity of released Yangtze sturgeons. The method includes: 1, preparation is carried out before an experiment; 2, adjustment and testing of water flows are carried out; 3, experiment sturgeons are introduced; 4, water flow training of the Yangtze sturgeons is carried out; and 5, finish time of the water flow training is carried out. According to the device, a river diversion screen and a submerged pump are installed in the middle of a cultivation bucket. A frequency converting controller is connected with the submerged pump through a wire, a water outlet of the submerged pump is connected with conduits, flow jetting holes are formed in the conduits, a water outlet pipe is installed in the middle of the cultivation bucket and has water level adjustment, and a water inlet pipe is connected on the upper portion of the cultivation bucket. By adoption of the training method, after the Yangtze sturgeons at different stages are trained, swimming capacities are all obviously improved. After training, water flow sensory capacities of the Yangtze sturgeons are improved, limiting swimming speed is high, and continuous swimming time in high-speed water flows is long.

The invention discloses an extracting method for a sturgeon skin collagen. The extracting method comprises the following steps: (1) preliminary treatment: taking and slicing a sturgeon skin, firstly immersing the sturgeon skin in alkaline solution, immersing the sturgeon skin in an acidic solution after rinsing, and then taking the sturgeon skin out for rinsing and standby; (2) extraction: adding water into the preprocessed sturgeon skin for leaching at 45 to 55 DEG C, and carrying out the filtration to remove impurities and obtain the collagen extracting solution; and (3) freeze drying: obtaining sturgeon skin collagen powder through the refrigerated centrifugation of the collagen extracting solution. The collagen product has higher quality. The extracting method has a higher collagen extraction rate, and not only improves the product yield, but also saves the cost in a simple way, thereby improving the economic benefits of a manufacturing enterprise.

The present invention discloses a PCR primer pair composition for identification or assisted identification of the sturgeon female parent and the sturgeon male parent, and applications thereof. The composition comprises a composition A for identification or assisted identification of the sturgeon female parent, a composition B for identification or assisted identification of the sturgeon parent, and a composition C for identifying or assistedly identifying whether the sturgeon female parent is Acipenser ruthenus, wherein the compositions A, B and C are separately packaged, the composition A comprises AGF, ABF, ABRM, DauF and SchF, the composition B comprises Atr-101, Atr-105, Atr-107, Atr-114, Atr-117, Eu-39, Eu-40, Eu-41, Afu19, Afu22, Afu39, Afu54 and Afu68, and the composition C comprises RutF.

The present invention discloses a technique for making sturgeon fry open mouth and making food-conversion breeding. Said technique is characterized by that according to the different stages of fry growth it can respectively use earthworm segment, whole earthworm and food to raise the sturgeon fry. Said invention also provides the concrete steps of said technique.

The invention discloses a cultivation method of sturgeon. The method comprises the steps as follows: firstly, a cement culture pond is sterilized for accommodating sturgeon fry; the sturgeon fry is put into the culture pond, and greenhouse cultivation of the sturgeon fry is performed; then the sturgeon cultivated in a greenhouse is transferred to deep water net cages in a reservoir for commercial sturgeon cultivation; and finally, scientific feeding is performed until commercial sturgeon appears in the market. A novel cultivation mode of cultivating the commercial sturgeon in the greenhouse and the net cages is provided, according to the method, power consumption on year-on-year basis is reduced by 70%-80%, the labor cost can be reduced by 50%, the culture cycle can be shortened by 15-20 days, water resources can be saved, and the survival rate of the sturgeon can be increased and can reach more than 96%. In conclusion, the whole cultivation method can reduce cultivation costs and risks, and accordingly, economic benefits are increased.

The invention relates to an underground water circulation water-saving type sturgeon ecological breeding method. The method includes the steps of pool preparation, pool disinfection, sturgeon stocking, breeding management and fishing. A water source used for the ecological breeding method is underground water, the underground water is pumped and aerated through an aeration tower and enters a breeding pool, breeding water acquired after circulating water treatment in water flowing out of the breeding pool enters the breeding pool again, and waste water is precipitated in a centralized mode and drained to farming irrigation ditch. Greenhouses are erected above the breeding pool. Water consumption almost ranges from 40 cubic meters per mu*hour to 60 cubic meters per mu*hour and is saved by 1 to 2 times compared with the water consumption being 120 cubic meters per mu*hour of local common sturgeon farmers; sewage acquired after circulating water treatment can be used for irrigating farmland, planted aquatic plants can generate certain economic benefits for the local, and an ecological breeding mode is formed.

The process of extracting sturgeon oil includes the following steps: grinding sturgeon maw and internal organs, adding 3-4 times of water and small amount of antioxidant, enzymolysis, stilling to delaminate, separating to obtain coarse sturgeon oil, and deacidifying, deodorizing and defatting coarse sturgeon oil to obtain refined sturgeon oil. The process has high sturgeon oil extracting rate, low peroxide number of the sturgeon oil product, high product quality, capacity of obtaining fish protein enzymolyzing matter also with the enzymolysis liquid, and other advantages.

The invention relates to a special compound feed for sturgeon, and a preparation method thereof. The special compound feed is prepared from the following materials according to weight percent: 28%-40% of bean pulp, 20%-25% of wheat middling, 25%-35% of fish meal, 7%-9% of fish oil, 6%-7% of shrimp powder, 0.2%-0.4% of salt, 0.1%-0.3% of bilineurine, and 0.9%-1.5% of special sturgeon feed additives, wherein the sum of the weight percent of the materials is 100%. By adopting the special compound feed for the sturgeon disclosed by the invention, the economic benefits of artificial breeding of the sturgeon can be obviously improved, the diseases, especially bacterial septicemia, a bacterial hemorrhagic disease and bacterial enteritis, in the artificial breeding process of the sturgeon can be obviously reduced, the survival ratio of the sturgeon is improved by 13%-16%, the growth of the sturgeon promoted, and the quality of the sturgeon can be improved. As the raw materials of the feed are cheap, the production and use costs of the special compound feed are relatively low and are saved.

The invention relates to a parent sturgeon feed. Through experiments, the use amounts of two essential fatty acids including EPA (Eicosapentaenoic acid) and DHA (docosahexaenoic acid) in a parent sturgeon feed, the weight part ratio of the two fatty acids and the content of the essential fatty acids in the total fat of the feed are confirmed, and comparison is carried out on organic trace elements and inorganic trace elements. The invention further provides a basic nutrition formula of the parent feed. Experiments prove that the feed disclosed by the invention is capable of obviously promoting secretion of female hormone in the parent sturgeon breeding period, and increasing the brood amount, egg hatchability and fertility rate of parent sturgeons, the growth speed, the weight and the survival rate of fries.

The invention discloses a method for artificial fertilization of sturgeon. The method comprises the following steps: checking the maturity of parent fish, collecting spermatic fluid, collecting fish roes, and carrying out artificial fertilization. Through the artificial fertilization method disclosed by the invention, the rate of fertilization is up to over 90%, and the fertilization of sperms and fish roes can be judged by naked eyes; and the unfertilized fish roes are removed, so as to greatly improve hatching rate. The artificial fertilization method effectively combines roe collection by squeezing and pressing and roe collection by in vivo manual incision, and is better than any one individual method. The disinfected parent fish of sturgeon can be re-placed into an incubation pond, and can regain health and recover ovulation capacity quickly.

The invention discloses a digitized monitoring method for a Chinese sturgeon natural reserve, belonging to an aquatic animal monitoring method which aims to solve the problems of data storage marks, underwater base station receiving and wireless transmission. The method has the technical scheme that acoustic marks, wireless marks and integrated passive repeater marks are adopted to singly mark or multiply mark young Chinese sturgeons. The method is characterized in that the center of the reserve is used as a base point; fixed float bowls are arranged every 7km in the north-south direction, and each float bowl is provided with a sonar signal receiving device; the signal receiving in the north-south direction covers a water area of about 60km wide; the sonar receiving device is used for receiving, and data signals are amplified and transmitted to a base station on land in a wireless transmission mode; a young Chinese sturgeon reserve is provided with 4-7 environment monitoring stations, and on-line water quality monitoring equipment is utilized to monitor the conditions of the hydrology, the meteorology, the water quality, and the like in real time; and obtained information is transmitted back to the base station in the wireless transmission mode. The method is used for Chinese sturgeon monitoring.

The invention belongs to the technical field of food processing, and particularly relates to a processing method for vacuum low-temperature preconditioned sturgeon flesh products. The processing method includecomprises the steps of compound thawing of raw materials, including Russian sturgeons and hybrid sturgeons, shaping, vacuum secondary pickling, low temperature pre-cooking, quick freezing, frozen storingage, thawing and cooking. The processing method has the advantages that special thawing liquid and the microwave technology are combined for thawing, so that the thawing efficiency is high, and the quality retaining effect is good; special pickling liquid and the vacuum secondary pickling technology are combined, so that the pickling speed is high, the processing loss is small, and themicrobial growth is effectively inhibited; through low temperature pre-cooking, the nutritional quality is maintained, and the flesh is evenly juicy; the sturgeon flesh products produced according tothe method have the advantages of tender and smooth taste, convenience in eating, rich nutrition, long shelf life and the like, thereby having a good market prospect.

The invention relates to the technical field of fishery culture, in particular to a sturgeon deep water net cage culture device. The device aims at solving the problems that a requirement on a growth temperature range in a sturgeon culture process is high, an over-summer survival rate particularly in China south lake and pond culture is low, and a feed coefficient of the existing net cage culture is high. The device comprises a net cage, and is characterized in that a net drawing device is arranged at the top of the net cage; one end of the net drawing device is fixed on a floating mat; a middle strand is arranged on the net cage; a steel plummet ring is arranged at the bottom of the net cage; and a meal table is arranged at the bottom of the net cage. The device can detect temperature automatically, meets requirements on sturgeon growth temperature in different seasons, particularly in a summer; the net cage is convenient and easy to clean and wash; feed is directly fed at the meal table at the bottom of the net cage, which is suitable for the characteristics of demersal fish and lower mouth of a sturgeon; and an entire water tank is good in stability in water.

The invention discloses a sturgeon culturing pond, which has the shape of square outer part and circular inner part, wherein the middle of the pond is the pond taken as a culturing region with straight edges and circular angles; the four square corners out of the pond with the straight edges and the circular angles are formed into four corner ponds which are respectively to be a first corner pond, a second corner pond, a third corner pond and a forth corner pond; the middle parts of the first corner pond and the second corner pond are provided with water charging openings which are communicated with water feed pipes and can charge water for the pond with the straight edges and the circular angles along the tangential direction of the arc pond walls of the corner ponds; the bottoms of the inner walls of the third corner pond and the forth corner pond are provided with water discharging openings which are communicated with the pond with the straight edges and the circular angles; the water discharging openings are provided with escape-proof networks for preventing the sturgeons from escaping; the upper edges of the outer walls of the third corner pond and the forth corner pond are provided with triangular-notch weir water discharging structures; the bottoms of the inner walls of the first corner pond and the second corner pond are respectively provided with a through hole which can be communicated with the pond with the straight edges and the circular angles; the through holes are provided with escape-proof networks; and the first corner pond and the second corner pond are internally embedded with oxygenating devices for increasing the oxygen content of water in the pond with the straight edges and the circular angles.

The invention discloses a hybrid sturgeon fingerling domestication and cultivation method. Mainly aiming at high sturgeon death rate in the high temperature period in summer and the phenomenon that sturgeons are hard to survive in summer in recent years, the invention provides a domestication and cultivation method which is more suitable for hybrid sturgeon fingerlings cultivated in the south. The method comprises the following steps: cultivating the hybrid sturgeon fingerlings to open mouth with live-bait for 7 to 8 days; feeding artificial compound feed to domesticate and cultivate the hybrid sturgeon fingerlings, and in the domestication and cultivation process, gradually adjusting the pH value of aquaculture water to be close to the pH value of water in the south; when the fingerlings grow to 8 to 10 cm, stopping domesticating and cultivating the hybrid sturgeons. The domesticated hybrid sturgeon fingerlings cultivated according to the method grow quickly and have a high survival rate and high economic benefits. The method provided by the invention has the advantages of simplicity and convenience in operation, low cost, easiness in popularization and the like.

The invention relates to the field of molecular biology and taxonomy and discloses a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) rapid detection method for common sturgeons as well as a primer and a kit for the rapid detection method. The PCR-RFLP rapid detection method for the common sturgeons comprises the following steps: firstly, extracting a genome DNA (Deoxyribose Nucleic Acid) of a sample to be detected as a template; secondly, carrying out PCR amplification reaction to obtain a PCR product by taking the genome DNA as the template; thirdly, digesting the PCR product by using restriction endonuclease to obtain a digested product; fourthly, carrying out agarose gel electrophoresis detection on the PCR product and the digested product and identifying species according to electrophoretogram. According to the PCR-RFLP rapid detection method disclosed by the invention, the simplicity in operation is realized; a small amount of fin rays or muscle is needed to be sheared on the basis of ensuring the survival of fingerling; the identification of fingerlings of the common sturgeons is quickly and accurately carried out; the accuracy and the reliability of the identification result are improved; the working efficiency is greatly increased.

The invention relates to a method for preparing sturgeon cartilage balls. The method comprises three steps of stuffing preparation, wrapper preparation and ball preparation. The invention simultaneously provides a treatment method for reasonably removing residual meat and removing fishy smell of fish bones, and provides a raw material formula of the balls with fresh taste. The invention also relates to the sturgeon cartilage balls prepared by the method.