479results about How to "Improve reproductive ability" patented technology

The invention provides a compound bio-fertilizer containing ardealite and application thereof. Compound bio-fertilizer is obtained through following steps: adjusting pH value of the ardealite to 4-5 via lime water, drying and crushing to 200-400 sections; drying animal manure, cutting crop straws into sections and then drying; mixing and fermenting the ardelite, the animal manure and the crop straws; controlling cultivation temperature to be at 28-32 DEG C after medium sterilization, sequentially inoculating various beneficial microorganisms, respectively cultivating the same, mixing mixed bacteria with prepared fermenting mixture by weight ratio of 1:300-1000 after completing cultivation, adding water to adjust water content to 35-45%, stirring the water , stacking the mixture for 3-5 days for co-fermentation, further adding water to adjust water content to 20-30%, and then crushing to obtain the compound bio-fertilizer. The compound bio-fertilizer is mainly applied to field crops and applied to improving saline-alkali soil, acid soil and coastal soil structures.

The invention relates to a preparation method for a high-activity humic acid biofertilizer. The high-activity humic acid biofertilizer comprises 97-99% of humic acid bio-organic fertilizer, 0.2-3% of special functional bacteria, and 0.1-0.3% of activator. The high-activity humic acid biofertilizer has high quality of organic matter nutrient, is excellent in soil conditioning function, has stable effect and long fertilizer efficiency, has balanced and complete nutrient, comprises multiple inorganic components needed by crops, has high fertilizer efficiency and can promote the growth of the crop and improve the yield and quality, has good disease resistance effect, improves the root system environment of the crop, improves the nutrient absorption of the crop, develops the root system of the crop, and enhances the stress resistance of the crop. After the high-activity humic acid biofertilizer is used by a peasant, a good planting effect can be obtained, and the yield and income are increased, so that the high-activity humic acid biofertilizer is used for producing environment-friendly and organic food.

The invention discloses a sludge aerobic composting method by utilizing composting compound microbial inoculum, which relates to a sludge aerobic composting method. The invention solves the problems of poor competitive power, long composting period and great nitrogen loss of the microbial inoculum in the prior sludge aerobic composting method. The method comprises the following steps: 1. carryingout amplification culture on microbial liquid A; 2. carrying out acclimation culture on the compound microbial inoculum A; 3. carrying out amplification culture on microbial liquid B; 4. carrying outacclimation culture on the compound microbial inoculum B; 5. taking dewatered sludge from a sewage plant, and mixing with conditioner; 6. inoculating the compound microbial inoculum, and composting; 7. and covering a layer of compound microbial inoculum B on the surface of the compost, and composting until the compost temperature reaches the ambient temperature, thereby finishing the sludge aerobic composting. The method shortens the time for the compost to reach the high temperature period by 2-3 days, shortens the composting period to 10-15 days, lowers the ammonia volatile amount of the compost by 30-50%, reduces the environmental pollution and enhances the fertilizer efficiency. The invention is applicable to sludge aerobic composting.

The speaker has a diaphragm main body 30 supported resiliently on a frame 23 via an edge portion 31 around its outer circumference, and the groove ribs 35 integrally formed in the edge portion 31, wherein a regulation member 37 for partially improving a flexural strength of the edge portion 31 is provided on a part of the front or back face of the edge portion 31.

The invention provides a preparation method of a microalga-based biochar material for heavy metal ion adsorption. The preparation method comprises the following steps that 1, microalgae and water are added into a hydrothermal reaction kettle to prepare microalga suspension liquid; 2, organic acid is added into the microalga suspension liquid, and after the hydrothermal reaction kettle is sealed, protective gas is introduced into the hydrothermal reaction kettle to replace air in the hydrothermal reaction kettle; 3, the hydrothermal reaction kettle in the step 2 is placed in a drying oven for a carbonation reaction, and the microalga-based biochar material is obtained; 4, activating treatment is performed on the microalga-based biochar material, and the activated microalga-based biochar material is obtained. According to the preparation method, biochar is prepared through hydrothermal carbonization by taking the nitrogen-containing microalgae as the raw material, and then activated modification is performed on the biochar through a KOH activating method and a NH3 activating method separately by taking the biochar as a precursor; the activated microalga-based biochar material obtained through activated modification has micro-mesoporous structures, the large specific surface area and the large pore volume, and the adsorption capacity of porous charcoal is greatly improved.

The invention belongs to the technical field of application biology and relates to a composite micro-ecological preparation for an aquatic product and a premix thereof. The efficient and multifunctional composite micro-ecological preparation is formed by combining multiple beneficial strains having different functions in a certain proportion; the preparation can obviously promote individual weight increment of an aquatic animal and improve yield per unit area and total yield, thereby increasing the income of aquaculture and improving benefits; and the preparation provided by the invention has the effects in multiple aspects of improving water quality, increasing algae in water, inhibiting harmful flora, improving the environment in the intestinal tract of an aquaculture animal, increasing feed and the like.

The invention provides a method for cultivating a novel Dingyuan pig breed, which relates to a method for cultivating a novel pig breed. The invention provides the method for cultivating the novel Dingyuan pig breed which has the advantages high fecundity, good meat quality and strong stress resistance of Dingyuan pigs as well as the characteristics of fast growth, high lean meat percentage and the like of imported pigs. The method comprises the following steps: a Dingyuan sow is hybridized with a novel USA strain Duroc boar to cultivate a first generation sow core group; second grading hybridization is performed to the first generation sow core group and a Dingyuan boar or the novel USA strain Duroc boar; from the third generation, group closure propagation and breeding are adopted respectively, and crossing of two to three generations is performed; and selection and remaining are performed through methods of comprehensive selection indexes and molecule marking assistance selection to form the novel Dingyuan pig breed, namely a novel breed of Wan Huai pigs and a novel breed of Dingyuan-Duroc pigs.

The invention provides a preparation method and application of a sea bdellovibrio bacteriovorus ecological preparation, and relates to a sea microbial ecological preparation and a preparation method of a bdellovibrio bacteriovorus preparation for mariculture. The preparation method comprises the following steps of: preparing a host bacteria suspension; separating and purifying bdellovibrio bacteriovorus; and propagating and culturing bdellovibrio bacteriovorus. The preparation method is characterized in that after pseudomonas stutzeri is connected with an incline and is activated, adding physiological saline in a test tube to prepare the bacterial suspension; then propagating and culturing with nutrient bouillon; adding a flocculating agent in the obtained bacterial suspension; standing and precipitating after uniformly shaking; adding the physiological saline to obtain 1012 cfu / mL of host bacteria suspension; separating and purifying the bdellovibrio bacteriovorus of collected sea and bottom sediment samples with a double agar plate method; pouring culture media containing the host bacteria suspension and the collected samples into sea agar in the lower layer for culture after uniformly mixing; and adding the host bacteria and the bdellovibrio bacteriovorus obtained by separation into sterilized natural sea, and culturing until the concentration of the bdellovibrio bacteriovorus is 108 to 109 pfu / mL for future use.

This invention relates to a micro-ecological agent and the preparation method thereof, belonging to the micro-ecological agent technological field. The preparation of the active bacteria agent of this invention comprises: using L-calcii lactas and four useful micro-organisms of clostridium bytyricum, Lactobacillus casei, bacillus subtilis and saccharomycete as characteristic elements, cultured purely or mixedly by means of universal fermentator, producing active bacteria powder by freeze-drying, and acquiring the finished products by double crossing of the powder at a specific proportion. By using the anaerobic and aerobic characteristics of related bacterial strains, this agent has the following merits: playing the synergy effect in enhancing the disease resistance of marine animals and the water purification of aquaculture, substituting antibiotics fully or partly for the disease prevention and cure in fresh water aquiculture and mariculture, and improving the economic benefits of aquaculture. The L-calcii lactase component can accelerate the husking growth and prevent the disease of soft husk of shellfish marine animals like shrimp and crab. This agent is characterized of being nontoxic, free from side effects, nonpersistent, without secondary pollution and no drug tolerance, and will have a wide commercial application in the fresh water aquiculture mariculture.

The present invention is directed to the construction of prototrophic, cellulo lytic strains of Saccharomyces cerevisiae with tethered and secreted cellulases and selection-based improvement of growth on cellulose by these strains. In some embodiments, host cells of the invention are able to produce ethanol using crystalline cellulose as a sole carbon source.

In the long-term space human living environment, the biological wastes from human, animals, and plants can be fully digested and recycled by rearing six kinds of recycling animals which will be nourishing feed stuff for feeding edible animals. These edible animals and their eggs combined with space plants will be various food for human. Water and nutrition left in the residues after rearing these recycling animals can be recycled and used to fertilize plants. All the animals and plants can be solidly reared in same cabin on the shelf with high densities in boxes, cages, trays and tanks which are all in minimum volume and weight. It is the basis for realizing mini space farm which provides a self-sufficient food regenerative system that enable humans to live and work in space independent of food supply from earth.

The invention discloses a composite microbiological preparation and a preparation method and use thereof. The composite microbiological preparation contains 20 to 35 percent of Pediococcus, 15 to 30 percent of bacillus, 25 to 40 percent of saccharomycetes and 5 to 20 percent of actinomycetes, and the total number of active bacteria in the preparation is more than 5 billion; and after mixed with protective agent and absorbent, the composite microbiological preparation can be packaged in liquid, solid or powder state. The microbiological preparation prepared by the invention has the advantages of high active bacterium concentration, less residual main fermentation nutrients in product, long quality guarantee period, obvious use effect, long lasting action and the like. The microbes in the preparation have high fertility, can form benefit predominant bacteria easily, can play an important microbiological balancing role in regulation of inherent nutrients, immunity, growth simulation, biological antagonism and the like in animal bodies, can increase the microecological self-regulating capacity of the animal bodies, prevent and reduce diseases and improve the health of hosts, and can be widely used as an additive for various kinds of feed, feed modifiers, water modifiers and soil modifiers.

The invention discloses a breeding method for a Puan black goat line. The breeding method utilizes foundation Guizhou black nanny goats and Nubia billy goats to bi-cross, first-class first Nubia billy goat-black nanny goat hybrid generation nanny goats chosen as the female parent and first-class Guizhou black billy goats chosen as the male parent backcross, first-class backcrossed filial generation billy goats and first-class nanny goats are chosen to cross to be fixed, and therefore the Puan black goat line with more stable genes is bred. By introducing the excellent genes of the Nubia goats, such as big build, high reproduction rate and fast growth, the breeding method ensures that the bred Puan black goat line has the advantages of big build, fast early growth and development, high reproduction rate, high meat productivity and the like besides the characteristics, such as high adaptability, high disease resistance and black clothing hair, of the Guizhou black goats.

The invention discloses a microbial agent, a preparation method thereof, and an application thereof, which relate to the technical field of microbial agent. The microbial agent includes Streptomyces albidofuscus CGMCC No.13717, Paecilomyces lilacinus CGMCC No. 13898, Streptomyces longus and mixed bacteria, wherein the mixed bacteria includes yeast, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Brevibacillus laterosporus and Trichoderma harzianum. Through combination of bacteria-fungi and the synergistic effect of multi-strains, the microbial agent has the advantages ofhigh activity of strains, high efficiency and multi-functions in treating waste water, waste gas or waste. In addition, the invention also provides a preparation method of a microbial agent, which adopts a double-layer immobilization (preliminary immobilization and secondary immobilization combination) method of the microbial agent to improve the stability of the microbial agent. The preparationmethod is simple in operation and easy to be popularized and applied in industry.

The invention relates to a high-activity humic acid composite microorganism fertilizer and a preparation method thereof. The high-activity humic acid composite microorganism fertilizer comprises the following components: 20-50% of activated humic acid, 0-20% of an organic material, 0.2-3% of a special-effect composite functional bacterial agent, 0.2-1.8% of a synergist, 30-60% of inorganic nitrogen phosphorus and potassium, 0.2-3% of trace elements and 0.1-0.5% of additives. The high-activity humic acid composite microorganism fertilizer integrates organic and inorganic nutrition and microorganism compounding and pelleting techniques, and the high-activity humic acid composite microorganism fertilizer has the characteristics of low water content, high hardness, availability in mechanical sowing and the like. The high-activity humic acid composite microorganism fertilizer prepared by the preparation method can be used for improving the soil fertility, improving the soil granular structure, fixing nitrogen in the air, decomposing phosphorus and potassium fixed in the soil, activating the micro-ecological environment of plant root soil, promoting growth and development of crops, enhancing the root system, increasing the utilization rate, reducing the use amount, reducing the soil-borne diseases, improving the anti-disease and stress resistance and increasing both production and yield.

The invention relates to a rehabilitation technology of polycyclic aromatic hydrocarbon pollutants (PAHs), particularly discloses a mixed inoculant for degrading PAHs. The mixed inoculant comprises indigenous mixed bacteria, wherein the indigenous mixed bacteria comprise Pseudomonas sp. B08, Pseudomonas sp. BM, Bacillus sp. BYB, Bacillus sp. B07, Ochrobactrum sp. ZHL4, Gordonia sp. BGDS and Mycobacterium sp. BFZG. The mixed inoculant disclosed herein can be added according to an inoculation quantity of 5-10%, and when the initial concentration of PAHs is 180.67mgL<-1>, the total degradation rate of PAHs after being processed for 9 days can reach 91.21%.

Disclosed is a method for extracting dihydro quercetin from larch. The invention is an improved production method. Due to different production materials and extraction processes, the dihydro quercetin products in U.S. and European markets are very expensive, at a price of 200-1000 euros per gram. Such a high market price economically limits the wide range of practicability of dihydro quercetin products. During the pretreatment of raw materials, 50 kilograms of larch wood chips and 500-800 liters of 50% ethanol solution are added into a reactor to get fully stirred and mixed; then the mixture is extracted and filtered for the second extraction; the filtrates are combined and adsorbed; the organic solvents are collected and dried by a vacuum evaporator through organic phase vacuum distillation; according to a weight proportion of 10:1, the residues are dissolved with hot water, decolorized with activated carbon and then crystallized at a temperature of 3-5 DEG C to precipitate the dihydro quercetin product which is then crushed after vacuum drying to finished product. The invention is used to extract dihydro quercetin from larch.

The invention discloses feverfew artemisia stolonifera, and in particular relates to an application of the Artemisia stolonifera in repairing of heavy metal cadmium polluted soil, namely planting the artemisia stolonifera in the heavy metal cadmium polluted soil. The enrichment of the artemisia stolonifera to the heavy metal cadmium is utilized, the artemisia stolonifera is planted into the polluted soil, the heavy metal cadmium can be absorbed and accumulated, and most cadmium can be transferred to the overground part; and the overground part is subjected to ashing treatment to extract the heavy metal, thus the aim of repairing the heavy metal cadmium polluted soil is achieved. The artemisia stolonifera has strong fertility, can grow rapidly and can grow on extremely poor soil, and management is extensive in the growing process of the artemisia stolonifera, thus the cost is low, the operability is high, and a new plant resource specie used for repairing the heavy metal cadmium polluted soil is developed.

The invention relates to a water circulating ecological system for aquaculture. The water circulating ecological system comprises an aquaculture main body ecological breeding tank, a biological purifying tank, a water purifying tank and a residue treatment ecological breeding tank. Environmental engineering for ecological breeding is constructed by the water circulating ecological system, and the breeding environment is controlled and optimized, so that aquaculture breeding dirt is cleared up by virtue of ecological mating of microorganism, plants and animals, and therefore, water self-cleaning effect of an ecological environment is achieved. By virtue of a recycling economy mode, the water is self-cleaned and utilized in a multi-stage manner to form an industrial chain of a plurality of products, so that duplex economic value added effect is produced. The production system ecologically controls the aquaculture, so that breeding water can be saved for over 70%, and water quality is regulated and optimized, and therefore, healthy aquaculture is implemented.

The invention particularly relates a ternary-hybridization pig breeding method, belonging to the technical field of vertebrate breeding. Due to the ternary-hybridization breeding method, the ternary-hybridization pig resists coldness, has strong disease resistance and strong fecundity and is high in lean factor and quick in growth speed. The ternary-hybridization pig breeding method comprises the following steps that: (1) by taking a black pig in Heilongjiang province as a female parent, and taking a wild boar in Great Khingan mountain range as a male parent, primary artificial hybridization is conducted to generate the second generation of sow; and (2) the second generation of sow and an American Duroc boar are subjected the secondary artificial hybridization to generate the third-generation pig.

The invention provides a loach protein antihypertensive peptide and a preparation method thereof, belonging to the technical field of biology. The antihypertensive short peptide separated and purified from an enzymolysis product of loach protein has high activity in inhibiting angiotensin converting enzyme (ACE). The preparation method provided by the invention comprises the following steps of: carrying out enzymolysis on the loach protein to prepare an enzymolysis liquid; separating and purifying the antihypertensive peptide from the enzymolysis liquid; and sequencing the antihypertensive peptide. The invention combines a modern enzyme engineering technique and a peptide purifying and identifying technique, deep development and utilization on the loach protein are utilized, and a novel-sequence short peptide Leu-Glue-Tyr with high ACE inhibiting activity is found; and the novel loach protein antihypertensive peptide provided by the invention has high ACE inhibiting activity and has an IC50 value of 0.103 mg / mL.

The invention provides a microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof, belonging to the technical field of environment restoration engineering. The strain utilized by the bactericide is Agrobacteriumsp. C13. The bactericide adopts a composite carrier which comprises turfy soil, saw dust, rice hulls and active carbon with the mass percentage being 30:25-30:25-30:15; the microorganism fermentation liquor is mixed with the composite carrier according to the mass ratio of 0.5-1:1; and the adsorption amount of the microorganisms of the composite carrier reaches 109-1012 per gram. The prepared bactericide is applied to the arsenic-polluted fields restored by ciliate desert-grass according to the use amount of 5g / kg, the biomass liveweight of the overground part of the restored plants is improved by 16-17%, and the removal rate of arsenic in the fields is improved by 40-41% in comparison. The carrier of the bactericide in the invention has rich raw materials, simple preparation method and easy realization of large-scale production.

The invention discloses artificial feed for Spodoptera frugiperda larvae and a preparation method and application thereof. The artificial feed for Spodoptera frugiperda larvae is made from, by weight,200-300 parts of corn meal, 80-120 parts of soybean meal, 10-30 parts of yeast powder, 10-20 parts of milk powder, 5-15 parts of vitamin complex, 6-9 parts of ascorbic acid, 2-6 parts of methyl 4-hydroxybenzoate, 2-5 parts of sorbic acid, 10-30 parts of agar powder, and 1500-2000 parts of sterile water. The invention also provides a method of feeding with the artificial feed. The artificial feedfor Spodoptera frugiperda larvae and the preparation method and application thereof have the advantages that materials are easy to attain, the cost is low, preparation is convenient, storage time is long, nutrition is complete, feeding effect is good, the artificial feed is very practical, and large-scale feeding of Spodoptera frugiperda larvae is benefited.

The invention aims to provide bacillus subtilis capable of producing chitinase and application thereof. The bacillus subtilis is obtained by plasma mutagenesis, has higher chitinase enzyme activity and protease enzyme activity, can be used as leavening agents, probiotics and preservatives, and can be widely applied to enzyme preparations and food industries. The preservation number of bacillus subtilis is CGMCC No.9166. The bacillus subtilis strain has relatively high chitinase enzyme activity and protease enzyme activity, is simple in nutrition requirement but has high fecundity, can be used as leavening agents, probiotics and preservatives, and has a wide application prospect.

The invention relates to the field of pollination and seed production of rapes, in particular to a method for pollinating and producing seeds of the rapes by osmia. The osmia is Japanese osmiacornifrons; and the method comprises the following steps: 1-2 days before the flowering of the rapes, moving beehives with bee releasing openings on one side into a rape seed producing field or a seed producing shed, wherein the distance between the adjacent beehives is 20-30 m and the distances between the lower end surfaces of the beehives and the ground are 1.2 m; and when the flowering period of the rapes is finished, finishing the pollinating task of the osmia, finishing bee rearing and collecting the bees into the beehives. Compared with the traditional bee pollination and seed-production, the method for pollinating and producing the seeds of the rapes by the osmia, provided by the invention, has the advantages as follows: (1) the seed producing efficiency is high and seed purity is good; (2) time and labor are saved once for all; (3) times are consistent and the operation is easy; and (4) the method is used for both scientific research and production, not only is suitable for requirements of large-area seed production fields but also can preferably satisfy small-area seed production sheds, in particular hybrid seed productions in the small-area seed production sheds; and difficulty problems of crossing, more labors, low seed setting rate and the like are effectively solved.

A method for culturing the hybrid scallop with high growth speed and stress resistance includes such steps as distant hybridization between Jiekong scallop and Huagui Jiekong scallop, hybridizing with Jiekong scallop JNR to obtain the male parent ZHJ, hybridizing with the female Jiekong scallop CDS5, and culturing the hybrid filial generation.

The method for breeding black rabbit includes the following steps: using local chesnut black male rabbit as male parent and using New Zealand female rabbit as female parent, makiing hydribization or artificial fertilization to obtain first-generation hybrid rabbit species, selecting excellent male rabbit and female rabbit to make direct cross or artificial fertilization to obtain second-generation hybrid rabbit species, then adopting similar method to obtain third-generation and fourth-generatin hybrid rabbit species, and utilizing fourth-generation male rabbit and female rabbit to make breeding. Said nvention adopts granular feed containing Chinese herbs of isatis leaf, lonicera flower, wild chrysanthemum flower and stemona root.

The invention relates to the field of aquatic baits and discloses an intensive production method for Mongolian Moina. Characteristics of the Mongolian Moina at all stages are known based on the comprehensive study on aspects of the Mongolian Moina, such as reproduction, development, ingestion, growth and the like. The Mongolian Moina is cultivated to achieve the intensive production by using an indoor cement pool and an outdoor pool, and a scientific and feasible practical technology which can also can utilized fully in production is formed, so that the current situation that a biological bait market is in short supply in the area in seedling production currently is alleviated.

The invention discloses a feed for reserved sows. The feed is prepared from the following components: corn, sorghum, bran, rice bran, grass meal, soybean meal, sunflower meal, corn germ meal, shell powder, fish meal, mushroom, decavitamin, a composite multi-mineral, and a core additive. The core additive is prepared from the following components: motherwort herb, herba epimedii, dodder, garlic, wolfiporia extensa, houttuynia cordata, and cnidium monnieri. The feed for the reserved sows can provide basic nutrients for the reserved sows; at the same time, with the addition of the core additives, the feed can guarantee the reserved sows have stronger breeding ability during a breeding period, and also can improve own immunity of the reserved sows; and the feed has the advantages of high security, wide raw material sources and low price.

The present invention relates to a microecological preparation for aquaculture and its preparation method. It is characterized by that said preparation method includes the following steps: firstly, respectively preparing acetobacter preparation, bacillus subtilis preparation, bacillus thuringiensis preparation and clostridium preparation, then uniformly mixing them according to a certain mixing ratio and using acetic acid to regulate pH value so as to obtain the invented microecological preparation for aquaculture.