Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

80results about How to "Short life cycle" patented technology

Low/high frequency shared leakage antenna, base station apparatus and close-range detection system using the antenna

The present invention provides a high / low frequency dual wireless location detection and information transmission system which is high in reliability and maintainability, and can be easily installed.A leakage coaxial inner conductor and a conductor line provided in parallel are short-circuited at an end, a single leakage coaxial operation and a loop operation of the inner conductor and the conductor line are performed at the same time, the ID of a tag is communicated using magnetic fields which locally exist near the conductor line by the loop, and wireless position detection and information communications which are less affected by the influence of ambient environments due to electromagnetic waves in a closed area are realized by open-type lines.
Owner:HITACHI LTD +1

Power-Conditioned Solar Charger for Directly Coupling to Portable Electronic Devices

The present invention relates to methods, tools and systems for manufacturing a durable and portable power-conditioned personal solar system charging apparatus. Various voltage and amperage matching algorithms are manipulated to particularize the personal solar system to power and / or charge an intended portable device or a set of intended portable devices having direct current (DC) load requirements. The optimized personal solar system that is matched to an intended device allows direct coupling to the intended device without the use of an internal battery or ancillary electronic circuit boards to distract the personal solar system output, and facilitates “fast” charging modes.
Owner:ADVANCE ENERGY SOLUTIONS

Resource configuration method and system

InactiveCN110147915AImprove the collaboration systemReduce difficultyResourcesTransmissionData matchingProcess configuration
The invention relates to a resource configuration method and system, being characterized by: carrying out the systematic sorting of complex resource configuration related elements through employing various technologies, carrying out the analysis of different dimensions, and setting a resource configuration system architecture model which can be referenced by an application-level service; assistingorganizations or individuals through innovation and reform; establishing a resource configuration system on the basis of an existing resource exchange system or no resource exchange system, so as topromote organizations and individuals, and individuals and individuals to establish a new cooperation mode under the condition of ensuring the legitimate identity and the data validity of networking,and ensuring the authenticity, the consistency and the non-tampering property of the data in different system transmission processes, thus realizing efficient utilization of data; and the data serviceand the intelligent service are combined with economy, resources and users to better research and distribute through communication with the server; and values are mined from big data such as geographic space, time, resources, users, reputations and demands, and data matching of resource demands and supply is established, and the association relationship between a regular mode and geographic lawsis acquired, and a sustainable and long-term-development complete business mode is created.
Owner:陕西爱尚物联科技有限公司

Mini Space Farm-A Food Regenerative System in the Long-Term Space Mission

In the long-term space human living environment, the biological wastes from human, animals, and plants can be fully digested and recycled by rearing six kinds of recycling animals which will be nourishing feed stuff for feeding edible animals. These edible animals and their eggs combined with space plants will be various food for human. Water and nutrition left in the residues after rearing these recycling animals can be recycled and used to fertilize plants. All the animals and plants can be solidly reared in same cabin on the shelf with high densities in boxes, cages, trays and tanks which are all in minimum volume and weight. It is the basis for realizing mini space farm which provides a self-sufficient food regenerative system that enable humans to live and work in space independent of food supply from earth.
Owner:ZHANG MAO

Low/high frequency shared leakage antenna, base station apparatus and close-range detection system using the antenna

The present invention provides a high / low frequency dual wireless location detection and information transmission system which is high in reliability and maintainability, and can be easily installed.A leakage coaxial inner conductor and a conductor line provided in parallel are short-circuited at an end, a single leakage coaxial operation and a loop operation of the inner conductor and the conductor line are performed at the same time, the ID of a tag is communicated using magnetic fields which locally exist near the conductor line by the loop, and wireless position detection and information communications which are less affected by the influence of ambient environments due to electromagnetic waves in a closed area are realized by open-type lines.
Owner:HITACHI LTD +1

Stand-Alone Solar Power Charger Directly Coupling to Portable Electronic Devices

InactiveUS20140261636A1Quickly and easily and inexpensively extend useful lifeLimited lifespanPhotovoltaic supportsBatteries circuit arrangementsElectrical batteryDirect coupling
A stand-alone solar power charger that may configured for direct coupling to a plurality of portable electronic devices. The solar power charger is particularized to power and / or charge an intended portable device or a set of intended portable devices having direct current (DC) load requirements. The solar power charger discharges energy without the use of an internal battery or ancillary electronic circuit boards, and facilitates “fast” charging modes. More specifically, the solar power charger incorporates a variety of features that make the design rugged, compact, waterproof, and durable.
Owner:SUNSTREAM TECH INC

Anti-aging evaluation method based on model organism caenorhabditis elegans and application of the method

The invention discloses an anti-aging evaluation method based on a model organism caenorhabditis elegans and an application of the method and relates to performance evaluation methods for phytochemicals, anti-aging medicines, healthcare products and the like. In the invention, under non-toxic concentration, the anti-aging effect of the to-be-tested substances is evaluated. With the evaluation method, existence of the anti-aging effect of the to-be-tested substances can be evaluated in a short period, wherein the evaluation is completed mainly through detection on life, heat stress capability,anti-ultraviolet irradiation capability, anti-oxidizing damage capability, reproductive capability, body length and lipofuscin content of the caenorhabditis elegans. The method is advantaged in that the model organism, caenorhabditis elegans, is short in growth period, so that the anti-aging effect can be observed rapidly in several weeks. The method has short experimental period and is convenientto carry out.
Owner:SOUTH CHINA UNIV OF TECH

Header shunting heat storage adverse current type heat exchanger

The invention discloses a header tank divided-flow heat-storage counter flow heat exchanger, which comprises a left header tank and a right header tank which are connected with a warm air water inlet, a warm air water outlet, a tap water inlet and a tap water outlet, wherein, a plurality of tap water heat-absorbing pipes are connected with the left and right header tanks; warm air heat supply pipes are connected through tap water heat-absorbing pipes; the warm air heat supply pipes are serially communicated with each other by heat supply communicating pipes; one end of each warm air heat supply pipe is connected with the warm air water inlet, while the other end is connected with the warm air water outlet; and a plurality of water insulation boards is alternatively connected the inside of the left and the right header tanks in order to separate the left and right header tanks to obtain the oriented flow channel. The counter flow heat exchanger of the invention has the advantages that: firstly the heat exchange efficiency is high and the temperature of the outlet water is high; secondly the joints of the heat supply pipes are arranged on the same level of axis line, which is suitably installed in household-measured users of the new heat supply pipe network unit, in particular to the needs of the three northeast provinces; thirdly the users can take a shower in the heat supply region of low temperature, and the heat-absorbing pipes at heat supply region of high temperature is free from scale formation; finally the counter flow heat exchanger is totally made of stainless steel, thereby saving brass pipe, reducing the cost and producing the clean water by exchange.
Owner:张伟

Time-triggered in-vehicle network message authentication method based on hierarchical multi-layer one-way key chain

The invention provides a time-triggered vehicle network message authentication method based on a hierarchical multi-layer unidirectional key chain, which belongs to the time-triggered vehicle network data authentication method under the Internet of Vehicles environment. The sending node is initialized, the sending node builds a hierarchical multi-layer one-way key chain and distributes the initial key; the sending node multicasts the verified message and key; the receiving node verifies the received message and key. The present invention is based on the concept of delayed release of hierarchical multi-layer key chains. The bottom key has a shorter release delay and length to authenticate real-time communication data, and each high-level key has a longer release delay and length. The length is used to credibly distribute the initial key of its lower layer key chain, and the sending node only needs to calculate and store the first key chain of each layer in advance; trigger the resource constraints, real-time and security of the vehicle network at time It provides an appropriate trade-off between performance and greatly reduces the overhead of bandwidth, storage and computing resources generated by message authentication.
Owner:JILIN UNIV

Agasicles hygrophila experimental population raising method

The invention discloses an agasicles hygrophila experimental population raising method. The agasicles hygrophila experimental population raising method includes steps that filter paper with the size equal to that of a bottle bottom is placed in an insect breeding bottle I, and is wetted, and 20-30 adult insects which are just subjected to the eclosion are placed in the insect breeding bottle I and are fed by three fresh alternanthera philoxeroides; alternanthera philoxeroides leaves with egg masses in the insect breeding bottle I are placed in insect breeding bottles II, each insert breeding bottle II holds ten alternanthera philoxeroides leaves, and then the insect breeding bottles II are placed in an artificial incubator; fresh alternanthera philoxeroides is placed in the incubator on the first day of incubation, and the alternanthera philoxeroides is continuously replaced by other fresh alternanthera philoxeroides until larvas are matured; and when about to pupate, the agasicles hygrophila matured larvas are transferred into pupating devices, each pupating device contains 100 matured larvas, the matured larvas enter straws of alternanthera philoxeroides to pupate, and pupating rate can reach 80% to 95%. The agasicles hygrophila experimental population raising method is simple in artificial raising, easy to control, small in occupied space and low in cost, and can be used for raising a controllable quantity of neat and consistent agasicles hygrophila with different insect development stages and different insect ages according to different experimental needs.
Owner:SHANXI AGRI UNIV

Authentication system used in distributed user service

The invention discloses an authentication system used in distributed user service. The system comprises an authentication center, a client, a server and custom made identity authentication devices, wherein the authentication center comprises an encryption storage medium and an encryption chip which are connected mutually; every two of the authentication center, the client and the server are connected, the client and the server are both connected with the custom made identity authentication devices through USB (Universal Serial Bus) ports, the encryption chip of the authentication center is used for processing encryption and decryption requests and generating a key, the encryption storage medium is used for storing the key information of all the custom made identity authentication devices, the identity authentication devices carry out encryption and decryption, and store keys of identity authenticators and keys used in communication, when encryption data communication is performed, two parties in each communication adopt keys to encrypt, the server and the authentication center as well as the client and the authentication center respectively adopt the corresponding keys, and the client and the server adopt a session key newly generated by the authentication center. The system has the advantages of safety and reliability. The hadden danger of data leakage is lowered.
Owner:南京中目网络科技有限公司

Method for extracting chitosan by employing flies

The invention relates to a method for extracting chitosan using fly, characterized in that the fly puparium is treated by acid alkaline process to obtain raw material. The raw material is subjected to decalcifying, deproteinizing, deacetylating, and then filtering, cleaning to be neutral to obtain the chitosan end product. The method can produce chitosan in enormous quantities using the high breeding property of fly, therefore the production rate of chitosan is increased. A product center combining raw material production and chitosan production together can avoid the inconvenience of raw material collection, therefore the production scale is greatly increased and the cost is reduced. During feeding the fly, the fly can be used for extract of protein feedstuff and antibiotic fly protein.
Owner:THE AFFILIATED HIGH SCHOOL OF SHANGHAI UNIV

Solid-state imaging pickup device, camera, automobile and monitoring device

A solid-state image pickup device is provided with a plurality of photoelectric conversion means for generating a signal corresponding to an incoming light quantity; a plurality of read out means for reading out the signal generated by each of the photoelectric conversion means; a transmission path for transmitting the signal read out by each of the read out means; an output means for outputting each signal transmitted through the transmission path from each of the read out means; and a generating means for generating a constant reference signal. The reference signal is outputted from the output means through at least a part of the transmission path.
Owner:PANASONIC CORP

Method and apparatus for binding domain name and specific service

InactiveCN101505305AFlexible topologyEffective Dynamic SettingsTransmissionDomain nameBinding domain
The invention discloses a method for binding a domain name and specific service, which comprises the following steps that: 1) a mapped domain name is generated for a user and specific service; 2) a service storage address corresponding to the domain name is set; 3) a service client requests the analysis of the domain name; 4) a domain name analysis server analyzes the domain name to the corresponding service storage address; and 5) the service client sends the request to the service storage address to acquire the specific service. The technical proposal maps each specific user and specific service to the specific domain name, and the subsequent access of the user is positioned to a preset server by analyzing the specific domain name, thereby achieving the aim of flexible network topology configuration and achieving complete and most effective load balance.
Owner:杭州比比西网络科技有限公司

Dry-method construction method for high-performance autolaved aerated concrete block wall

InactiveCN106013509AHigh strengthAdhesive, special adhesive has strong bonding forceWallsBuilding material handlingSurface finishThermal bridge
The invention relates to the technical field of construction, and inparticular relates to a dry-method construction method for a high-performance autolaved aerated concrete block wall. The dry-method construction method comprises the following process procedures: performing construction preparation, positioning and paying off, leveling a wall foundation, erecting story poles, placing wall tie bars, constructing a block wall body, laying pipelines inside the wall body, mounting door / window hole cross beams, constructing outer wall concrete thermal bridge parts, constructing an outer wall waterproof interface agent, pasting special plaster putty on the inner wall in batch, constructing an inner wall finish coat layer, pasting wall surface heat-preservation blocks, and constructing a roof surface finish coat layer. The dry-method construction method is characterized in that a dry-method construction process is adopted for a blocking wall body, the part between the wall body and a beam top is filled with a polyurethane foamed adhesive, moreover a special construction method of an outer wall concrete thermal bridge part is adopted, the common quality problems of creep change of conventional masonry mortar and mortar joint cracks along wall surfaces are effectively solved, the overall property, the shock resistance and the heat-preservation property of the wall are improved, and the dry-method construction method can be widely applied to production construction of various buildings.
Owner:HUBEI SHENZHOU BUILDING MATERIAL

Node link scheduling method of heterogeneous integrated power consumption information collection network

The invention discloses a node link scheduling method of a heterogeneous integrated power consumption information collection network. The optimum clustering amount of a network region is determined according to the energy consumption of nodes in the network region; a plurality of cluster head nodes are selected in the network region; when the amount of the cluster head nodes is less than or equal to the optimum clustering amount, slave nodes in the network region are added in matched clusters according to a minimum distance criterion; the communication modes of the slave nodes in each cluster are determined according to an energy consumption balancing criterion; and finally, the slave nodes transmit data according to the communication modes. According to the method, through adoption of hybrid communication modes, the energy consumption in the heterogeneous integrated network region is balanced.
Owner:ELECTRIC POWER SCI RES INST OF STATE GRID XINJIANG ELECTRIC POWER +2

Method for preparing protein feed and protein feed additive by culturing fly maggots with defined medium

The invention discloses a method for preparing protein feed and a protein feed additive by culturing fly maggots with a defined medium. The fly maggot protein feed is prepared through the steps that the defined medium is inoculated with cyclorrhapha flyblows, culturing is performed for 2-6 days, and then drying is performed; the defined medium is prepared from wheat bran, corn flour, soybean meal and plant fiber powder. According to the method, the fly maggots cultured by the defined medium are adopted as raw materials for preparing the protein feed and the protein feed additive, and therefore continuous breeding all year round can be achieved; the raw materials of the medium are all selected from agricultural leftovers and are high in yield and low in cost, and therefore long-term breeding can be achieved; the defined medium used in the formula is comprehensive and balanced in nutrition and meets the all-aspect fly maggot growth requirements that carbohydrate and an appropriate amount of grease are supplied to fly maggot growth, and maggot protein obtained through culture is beneficial for digestion and absorption of livestock and aquatic animals.
Owner:ZHONGSHAN UNICARE NATURAL MEDICINE +1

Logic-compatibility-based digital circuit fault diagnosis method and system

The invention discloses a logic-compatibility-based digital circuit fault diagnosis method and a logic-compatibility-based digital circuit fault diagnosis system. The method comprises the following steps of: building a diagnosed digital circuit system by a user through a digital circuit graphical modeling unit, then setting the parameters of the circuit by using a description configuration unit, expressing a digital circuit graph by using an AD2L language and storing the digital circuit graph in a file form; reading the digital circuit description file stored by the description configuration unit by the user through an AD2L language resolving unit, performing front and rear propagation through a theorem proving unit by using the parameters provided by the user, and recording the conflict; performing diagnosis and solving by using a diagnosis algorithm unit; and highlighting a suspicious component on the graphical digital circuit by using a fault positioning unit, and generating a diagnosis test report. The system has the functions of conflict identification and candidate generation and detection, the whole operating process is simple and spends short time, and judgment and positioning of the system on fault points do not depend on experience of operators or experts.
Owner:DALIAN MARITIME UNIVERSITY

Method for curing fishes through negative pressure and ozone cold sterilization

The invention provides a method for curing fishes through negative pressure and ozone cold sterilization, in particular the method for curing red oil fishes through negative pressure cold sterilization. The method comprises the following steps of: putting preprocessed raw material fishes into a sealed container; adding salt in an amount which is 1 to 15 percent based on the mass of the fishes into the container; regulating the vacuum degree in the container to be 0.01 to 0.065MPa; rolling and kneading for 5 to 10 minutes at the rotating speed of 10 to 20 r / m; introducing ozone to ensure that the concentration of the ozone is 2.0 to 50ppm; rolling and kneading for 5 to 60 minutes at the rotating speed of 10 to 20 r / m; regulating the vacuum degree of the container to be 0.07 to 0.095MPa; and continuously curing for 4 to 24 hours to obtain the processed fish. The method has the advantages that the method has good effect of guaranteeing the quality by adopting negative pressure and ozone cold sterilization curing technology; compared with a product cured by a normal pressure curing method, the product cured by the process has lower content of volatile basic N, histamine and trimethylamine; and the method can obviously improve the organoleptic physical property and the edible safety of the product, also has the effect of effectively killing microorganisms in the raw material fishes and can improve the curing efficiency.
Owner:ZHEJIANG UNIV OF TECH

DNA constructs that contain Helianthus annuus Hahb-10 gene coding sequence, method for generating plants with a shortened life cycle and a high tolerance to herbicidal compounds and transgenic plants with that sequence

ActiveUS20070234439A1Positive regulatorImprove elongationSugar derivativesClimate change adaptationGMO PlantsTransgene
The present invention refers to a gene from Helianthus annuus encoding a transcription factor that comprises a homeodomain associated with a leucine zipper. This gene is named Hahb-10. The transcription factor Hahb-10 can be used in DNA constructs to transform host cells and plants. Transgenic plants that overexpress this transcription factor are more tolerant to herbicides, and have a shorter life cycle.
Owner:NATIONAL UNIVERSITY OF THE LITTORAL +2

Chinese medicinal preparation, preparation and quality control method thereof

The invention discloses a traditional Chinese medicine preparation, including the traditional Chinese medicine active ingredients and the pharmaceutically acceptable amount of medicinal excipients, the unibract fritillary bulb is smashed into coarse powder, and the unibract fritillary bulb extract liquid is obtained by collecting the percolation liquid and recycling the ethanol according to the percolation methods under the fluid extract and the extract items (appendix IO of Part one of Chinese pharmacopeia 2005 version); the pummelo peel, dried ginger and liquoric root are respectively immersed by ethanol solution and filtered, the filtrate is subject to ethanol recycling and is condensed into thick paste A; the loquat leaf, coastal glehnia root, Indian buead, platycodon root, rhizoma pinelliae preparata, snakegourd fruit and bitter apricot seed are boiled by adding water for 2 to 3 times and 1 to 3 hours for each time, the decoction solution is merged, placed still and filtered, the filtrate is evenly mixed with unibract fritillary bulb extract liquid and the thick paste A, then the menthol ethanol solution and apricot water are added for even mixing, and the medicinal excipients are added to prepare the preparation. The traditional Chinese medicine preparation which is prepared by the invention has the advantages of significant efficacy, high bioavailability, rapid onset of action, convenient administration and easy acceptation by patients, and the invention can be used for the treatment of cough, expectoration or ungratifying expectoration, dry pharynx and mouth and other symptoms.
Owner:江西药都樟树制药有限公司

Identification method of poisonous substances in water from urban domestic wastewater treatment plant

The invention discloses an identification method of poisonous substances in water from an urban domestic wastewater treatment plant, and belongs to the field of wastewater treatment. The identification method provided by the invention comprises the following steps of (1) carrying out acute toxicity biological detection by adopting daphnia magna so as to detect the toxicity of the wastewater; (2) detecting the suspected toxic substances in the wastewater; and (3) determining the main toxic substances in the wastewater, namely carrying out nitrogen blow-off treatment for 2h, carrying out nitrogen blow-off treatment for 2h after regulating the pH to 6, and regulating the pH to 6 for carrying out nitrogen blow-off treatment for 2h and then regulating the pH to 8.5, therefore, the main toxic substances, causing microbiological poisoning incidents of a biotreatment system, in the wastewater can be determined by detecting the change of the semilethal rate 24h-LC50 of the daphnia magna in a water sample after the three treating steps are performed and by adding the suspected toxic substances. The identification method of the poisonous substances in water from the urban domestic wastewater treatment plant provided by the invention has the advantages that the cost is low, and the period is short.
Owner:广州市净水有限公司

Closed-loop control device of a mechanical sewing machine and method for controlling the same

A closed-loop control device of a mechanical sewing machine includes an isolated switch-mode power supply module, a DC motor, a speed control module, a processor, an electronic switch, a current detection module and a voltage detection module. The isolated switch-mode power supply module rectifies an AC power to a DC power and supplies the DC power to the DC motor. The speed control module sends a speed signal to the processor. The processor adjusts an output voltage to the DC motor according to the speed signal. The current detection module and the voltage detection module further detect an operating current signal and an operating voltage signal of the DC motor for the processor to control a turn-on time of the electronic switch to adjust an average operating voltage of the DC motor according to the operating current signal, the operating voltage signal and the speed signal.
Owner:CHEN HSUAN TAI

Production and processing method for edible camellia seed oil with low acid value and high quality

The invention discloses a production and processing method for edible camellia seed oil with low acid value and high quality. The production and processing method comprises multistage separation supercritical CO2 extraction and molecular distillation refining. According to the invention, through multistage separation supercritical CO2 extraction for camellia seed oil, high extraction rate is maintained; due to multistage separation, simultaneous separation of water, free fatty acid, odor components, pigment components and the like can be realized; and through refining treatment with a molecular distillation technology, the edible camellia seed oil with low acid value and high quality is obtained, and the edible camellia seed oil can reach an oil yield of 90% or above, an acid value of lessthan 0.6 and an unsaturated fatty acid content of 90% or above, and is rich in natural anti-oxidant active components like vitamin E, squalene and beta-sitosterol. The production and processing method provided by the invention avoids traditional technical processes of degumming, deacidifying, decolorizing, dewaxing and deodorizing, and has the advantages of high oil yield, short life cycle, avoidance of product oxidation and rancidification, retention of nutrient components in oil, no flammable and explosive hazards, no environmental pollution, no solvent and heavy metal residues, natural quality ensurance of camellia oil, etc.
Owner:广东金妮宝科技发展有限公司

Semi-Continious Non-Methane Organic Carbon Analyzer

An instrument to provide on-site analyses utilizing a combination cryotrap and absorbent traps in the extraction of constituents from the sample gas stream consistent with the requirements of 40 CFR Pt. 60 App. A Method 25.The air sample is passed through a cryotrap system which is maintained at the temperature of dry ice ˜72° C.). Heavier molecular weight constituents in the gas sample are condensed on the cold surfaces of the trap. The lighter molecular weight constituents are retained in the absorbent trap, while also acting as a column to separate methane, carbon dioxide, and carbon monoxide from each other and the remain carbon compounds.After separation, all of the sample constituents are oxidized to carbon dioxide and reduced to methane prior to being introduced to a Flame Ionization Detector, which measures the concentration of each against known calibration standards.
Owner:STOLLINGS WAYNE ALLEN

Preparation method and application of cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging life of caenorhabditis elegans

InactiveCN110326785AAvoid loss of functional activityGood biocompatibilityAccessory food factorsFood shapingFreeze-dryingSlurry
The invention discloses a preparation method of a cationic marine polysaccharide-anthocyanin polyelectrolyte compound capable of prolonging the life of caenorhabditis elegans. The preparation method comprises the steps that bilberry anthocyanin is taken to be dissolved into an acetic acid aqueous solution with the volume fraction being 1%-2%, and thus a solution A with the bilberry anthocyanin concentration being 0.2-2 mg / ml; a wall material is added into the solution A, and ultrasonic dispersion and slurry homogenizing are conducted to obtain a solution B; the wall material is prepared from chitosan and pectin according to the weight ratio of (1:1) to (1:3); the weight ratio of the bilberry anthocyanin to the wall material in the solution A is (2:1) to (2:4); and the solution B is subjected to vacuum freeze-drying, and thus the cationic marine polysaccharide-anthocyanin polyelectrolyte compound is obtained. Through the prepared cationic marine polysaccharide-anthocyanin polyelectrolyte compound, the life of the caenorhabditis elegans can be prolonged, and the reproductive capacity can be enhanced; and the marine polysaccharide-anthocyanin polyelectrolyte compound can be used for preparation of food and health care products, drug loading, drug slow release and the like.
Owner:DALIAN POLYTECHNIC UNIVERSITY

Tetrahymena expression vector, construction method and application of one-step introduction of exogenous gene

The invention discloses a Tetrahymena expression vector for introducing exogenous genes in one step, its construction method and application. Plasmids HSP702-GFP and THX-HGFP were used to construct the intermediate vector TH-HGFP through PCR and enzyme digestion ligation methods; plasmids TH-HGFP, PET23a-GST and pCDNA3.1-FLAG were used to construct the intermediate vector TH-HGFP through PCR and enzyme digestion ligation methods, respectively. Construct intermediate vectors TH-HIS-HGFP, TH-GST-HGFP and TH-FLAG-HGFP; use the above intermediate vectors and plasmid pD5H8 to construct Tetrahymena expression vectors: pD5H8-HGFP, pD5H8 through PCR and enzyme digestion. -HIS-HGFP, pD5H8-GST-HGFP, pD5H8-FLAG-HGFP. This series of vectors all contain green fluorescent screening marker HGFP, which can be used for high-throughput screening of recombinants; this series of vectors can replace the cassette structure HGFP contains reverse I-SceI rare enzyme cutting sites on both sides to introduce foreign genes At this time, the foreign gene fragment with the I-SceI linker is connected to the series of vectors treated with I-SceI in one step, and the Tetrahymena expression vector containing the foreign gene can be obtained. The invention is easy and quick to operate. After the Tetrahymena expression vector introducing the exogenous gene is transfected into Tetrahymena, the efficient expression of the exogenous gene can be achieved.
Owner:INST OF AQUATIC LIFE ACAD SINICA

Annual konjak corm flower initiation inducing method

The invention relates to an annual konjac corm flower-formation induction method and belongs to the technical field of the agriculture biology, which comprises a selection of the konjac corm, immersion of the growth adjusting agent and spraying procedures; a. the konjac corm tissue cultured seedling is moved out of the bottle in April, and is planted in the specification of 10 cm multiplied 10 cm, and is harvested in November then; 150 grams of annual corm is selected to be stored in dark under the temperature of 20 DEG C minus or plus 2; b. in March next year, the corm is immersed in a plant growth adjusting agent-gibberellin 100ppm for one hour, and is taken out for drying, and is planted in a flower pot one week later; c. when the top bud of the corm is grown to 5 centimeters, the corm is sprayed with 1 ppm plant growth adjusting agent-gibberellin, thereby the annual konjac corm is flowered in May. Compared with the prior art, the invention has the advantages that the flowering of the konjac corm is earlier and the living period of the konjac is shorter, which establishes a foundation for accelerating the konjac system classification research and the hybrid breeding work.
Owner:云南省农业科学院生物技术与种质资源研究所

Apparatus and Method for Energy Recovery

There is provided a heating system for a building comprising; i) a heater; ii) a media flow passage through which a medium heated by the heater passes in order to distribute heat through the building; and iii) a heat recovery apparatus for recovering latent heat of adsorption from a humid gas. The heat recovery apparatus comprises a) a gas flow passage connected at one end to a source of humid gas; b) a desiccant material provided within the gas flow passage such that the humid gas contacts the desiccant material and water vapour is adsorbed releasing latent heat of adsorption; wherein the media flow passage and gas flow passage are in heat exchanging relationship, such that when the desiccant material adsorbs water vapour from the exhaust gas the latent of the adsorption is at least partially transmitted to the medium.
Owner:XSORB ECO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products