Anti-aging evaluation method based on model organism caenorhabditis elegans and application of the method

A technology of Caenorhabditis elegans and an evaluation method, which is applied in the field of anti-aging evaluation based on model organism Caenorhabditis elegans, can solve the problems of aging and shorten the lifespan of mice, and achieve a short life cycle, short experimental period and easy operation. Effect

Inactive Publication Date: 2018-04-06
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, experimental studies in mice have shown that overexpression of P53 can induce aging and greatly shorten the lifespan of mice

Method used

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  • Anti-aging evaluation method based on model organism caenorhabditis elegans and application of the method
  • Anti-aging evaluation method based on model organism caenorhabditis elegans and application of the method
  • Anti-aging evaluation method based on model organism caenorhabditis elegans and application of the method

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Embodiment 1

[0059] 1. Preparation of blueberry extract

[0060] Choose fresh blueberries and wipe the surface to avoid damage. Weigh 20 g of washed blueberry fresh fruit and mix it with 200 mL of acetone solution with a volume fraction of 80%, use a tissue masher to crush and extract the active ingredients in blueberry fresh fruit for 5 min, then transfer the solute and residue to a beaker, and then use Homogenizer for 3 min, rotating speed 6000 r / min. After rotary evaporation, make up to volume with 25 mL of 70% alcohol, and store at -40°C after aliquoting.

[0061] 2. Anti-aging evaluation method based on model organism level Caenorhabditis elegans

[0062] 1. Activation and plating of E. coli OP50

[0063] (1) Turn on the UV lamp of the ultra-clean workbench for 5 minutes, and then turn off the UV lamp to start aseptic operation. Take a sterilized 15 mL centrifuge tube, add 10 mL of sterilized LB liquid medium to it with a pipette, and then add 100 μL of E. coli OP50 to it. The 15...

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Abstract

The invention discloses an anti-aging evaluation method based on a model organism caenorhabditis elegans and an application of the method and relates to performance evaluation methods for phytochemicals, anti-aging medicines, healthcare products and the like. In the invention, under non-toxic concentration, the anti-aging effect of the to-be-tested substances is evaluated. With the evaluation method, existence of the anti-aging effect of the to-be-tested substances can be evaluated in a short period, wherein the evaluation is completed mainly through detection on life, heat stress capability,anti-ultraviolet irradiation capability, anti-oxidizing damage capability, reproductive capability, body length and lipofuscin content of the caenorhabditis elegans. The method is advantaged in that the model organism, caenorhabditis elegans, is short in growth period, so that the anti-aging effect can be observed rapidly in several weeks. The method has short experimental period and is convenientto carry out.

Description

technical field [0001] The invention belongs to the technical field of animal models, and in particular relates to an anti-aging evaluation method and application based on model organism Caenorhabditis elegans. Background technique [0002] Aging is defined as the loss of functions and adaptations to the environment. The social concept of aging encompasses the loss of independent living abilities due to mobility, consciousness, and sensory functions. Aging can lead to the occurrence of diseases including tumors, cardiovascular diseases, metabolic diseases and neurodegenerative diseases. Due to these conditions, the respect and worship of the elderly has been delayed, stopped, and even reversed. Antidrugs marketed to delay the side effects of aging through diet, exercise, supplementation, and hormone therapy have received widespread attention. [0003] Aging is a process regulated by many factors, and many theories have attempted to explain this process. So far, the resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0008
Inventor 郭新波王怀玲刘瑞海
Owner SOUTH CHINA UNIV OF TECH
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