Anti-aging evaluation method based on model organism caenorhabditis elegans and application of the method
A technology of Caenorhabditis elegans and an evaluation method, which is applied in the field of anti-aging evaluation based on model organism Caenorhabditis elegans, can solve the problems of aging and shorten the lifespan of mice, and achieve a short life cycle, short experimental period and easy operation. Effect
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[0059] 1. Preparation of blueberry extract
[0060] Choose fresh blueberries and wipe the surface to avoid damage. Weigh 20 g of washed blueberry fresh fruit and mix it with 200 mL of acetone solution with a volume fraction of 80%, use a tissue masher to crush and extract the active ingredients in blueberry fresh fruit for 5 min, then transfer the solute and residue to a beaker, and then use Homogenizer for 3 min, rotating speed 6000 r / min. After rotary evaporation, make up to volume with 25 mL of 70% alcohol, and store at -40°C after aliquoting.
[0061] 2. Anti-aging evaluation method based on model organism level Caenorhabditis elegans
[0062] 1. Activation and plating of E. coli OP50
[0063] (1) Turn on the UV lamp of the ultra-clean workbench for 5 minutes, and then turn off the UV lamp to start aseptic operation. Take a sterilized 15 mL centrifuge tube, add 10 mL of sterilized LB liquid medium to it with a pipette, and then add 100 μL of E. coli OP50 to it. The 15...
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