The invention discloses a sleep mask with functions of acne treatment and beautifying and a preparation method of the sleep mask. The sleep mask is prepared from peach blossoms, Hylocereus undatus, lycopene, rhizoma dioscoreae and potato juice, aloes, bee propolis, Herba Centellae, vitamin E, green tea extract, kelps, Cortex Dictamni, calendula, pearl powder, tetracycline and tea tree oil. The sleep mask is nonirritant and free of anaphylaxis, the method is convenient to operate, simple and feasible, and cheap raw materials, low cost, low energy consumption and high product added value are realized. The peach blossoms are rich in vegetable proteins and free amino acids easy for skin to absorb, evident effects of prevention and treatment of dry skin, rough skin, wrinkles and the like are realized, blood circulation can be improved, skin nutrition and oxygen supply is promoted, and excretion of lipofuscin which accelerates aging is accelerated. In addition, the raw materials include not only foods and vegetables with the function of acne mark removal but also medicinal materials with the function of acne mark removal, so that effectiveness in acne mark removal is achieved.