29 results about "Flavanone" patented technology

The invention discloses recombinant saccharomyces cerevisiae, a 11-seed oil fermentation product as well as a preparation method and application of the 11-seed oil fermentation product, and belongs to the technical field of synthetic biology and fermentation product preparation. According to the recombinant saccharomyces cerevisiae disclosed by the invention, a caffeic acid coenzyme A ligase 4CL coding gene, a chalcone isomerase CHI coding gene, a flavanone-3-hydroxylase F3H coding gene and a chalcone synthase CHS mutant coding gene are over-expressed, and the chalcone synthase CHS mutant is preferably Y69H / H71Y / Q161K. The preparation method of the 11-seed oil fermentation product comprises the following steps: fermenting a recombinant saccharomyces cerevisiae fermentation solution in the presence of a sophora flower bud extract and caffeic acid to obtain a first fermentation solution; fermenting the lactic acid bacteria fermentation liquor in the presence of 11 seed oil to obtain second fermentation liquor; and mixing the first fermentation liquid and the second fermentation liquid, continuing fermentation, and respectively collecting an oil phase and a water phase after fermentation is finished, so as to obtain a fermentation product. The product provided by the invention has multivitamin antioxidant activity.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

This invention discloses 2'-hydroxy-4,4',6'-trimethoxychalcone and 5,7,4'-trimethoxyflavanone sulfonamide derivatives, their synthesis methods and applications, belonging to the field of pharmaceutical technology, and involving two series of compounds: 3-sulfonamido-2'-hydroxy-4,4',6'-trimethoxychalcone and 8,3'-disulfonamido-5,7,4'-trimethoxyflavanone, and their preparation methods. Starting with 2-hydroxy-4,6-dimethoxyacetophenone and p-anisaldehyde or 4-methoxy-3-nitrobenzaldehyde, 2'-hydroxy-4,4',6'-trimethoxychalcone or 3-amino-2'-hydroxy-4,4',6'-trimethoxychalcone intermediates were synthesized via the Claisen-Schmidt reaction. Then, through sulfonation and intramolecular Michael addition reactions, 2'-hydroxy-4,4',6'-trimethoxychalcone and 5,7,4'-trimethoxyflavanone sulfonamide derivatives were synthesized. The preparation method is simple, mild, and yields high results. In vitro and in vivo activity tests show that the derivatives disclosed in this invention have significant in vitro inhibitory activity against gastric cancer, esophageal cancer, colorectal cancer, and lung cancer cell lines, acting as inhibitors of the protein YBX1 / RPN1. Clinically, they can be applied to targeted therapy for cancers such as gastric cancer, esophageal cancer, colorectal cancer, and lung cancer.

The invention relates to application of flavanone as a bactericidal synergist in plant disease control. Specifically, the bactericidal synergist, a substituted aniline bactericide, a methoxy acrylate bactericide, a triazole bactericide and a substituted benzene bactericide are jointly applied to prevention and treatment of diseases. Flavanone has a synergistic effect on the bactericide, and can enhance the toxicity of the bactericide, improve the sensitivity of a drug-resistant strain to the bactericide and promote the control effect of the bactericide on indoor or field drug-resistant phytopathogens when being mixed with the bactericide, and can effectively reduce the dosage of the bactericide. And a new method is provided for the development and application of the existing new bactericide preparation.

A composition for forming a protective film for a wet etching solution for semiconductors, comprising: component (A): a film-forming component; component (B): a compound selected from the group consisting of a condensed tannin (b1) obtained by polymerizing a compound having a flavanol skeleton, a hydrolyzable tannin (b2) obtained by ester-bonding a sugar and an aromatic compound of gallic acid or ellagic acid, and a flavonoid (b3) derived from a compound having a flavanone skeleton structure; and component (C): a solvent.

The invention discloses a gingko GbF3H gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbF3H gene is obtained by analyzing and screening on the basis of ginkgo transcriptome data in the earlier stage of a research group, the GbF3H gene is flavanone 3-hydroxylase, the nucleotide sequence of the GbF3H gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbF3H gene is shown as SEQ ID NO.2. A GbF3H overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the difference of 28 flavonoid metabolites is obvious, and the contents of 9 flavonoid substances such as formononin are up-regulated. The invention provides a theoretical basis, a valuable gene resource and a method for analyzing a ginkgo flavone synthesis mechanism and improving the flavone yield through metabolic engineering, and has important significance on development and utilization of ginkgo resources and cultivation of new germplasm with medicinal value.

The invention belongs to the technical field of medicinal chemistry and pharmacology, and particularly relates to a flavanone thiazole derivative as well as a preparation method and application thereof. The structural formula of the flavanone thiazole derivative is shown in the specification, and in the structural formula, R represents one of 2-iodophenyl, 2, 4-dichlorophenyl, 4-fluorophenyl, 2-bromobenzyl, 4-bromophenyl, 2-bromophenyl, 4-chlorobenzyl and 4-methylphenyl. The flavanone thiazole derivative is a flavanone thiazole ring-fused derivative, and pharmacological research shows that the compound has high liver cancer cell proliferation inhibition activity and can be applied to preparation of drugs for preventing and treating liver cancer.

This invention discloses a method for preparing flavanone extracts through microbial co-fermentation and its application in lipid-lowering activity, belonging to the field of food nutrition and functional components. By co-fermenting orange peel raw materials with yeast and Monascus purpureus, the synergistic metabolic effects of different microorganisms are utilized to increase the content and reconstruct the composition of flavanones from orange peel. The bioactivity of the fermentation products was evaluated using a mouse 3T3-L1 preadipocyte differentiation model. The results showed that the flavanones from orange peel treated with co-fermentation had a significant inhibitory effect on lipid accumulation, and their lipid-lowering activity was significantly better than that of unfermented or single-strain fermentation products. The method of this invention is simple, reproducible, and suitable for large-scale production, and can be applied to the development of lipid-lowering functional health products or dietary supplements.

The invention discloses an application of a compound or a composition thereof as a semen cryopreservation solution. According to the invention, through whole genome re-sequencing and seminal plasma non-target metabonomics, a regulation mechanism of interaction of genome variation and metabolites on semen traits is analyzed. Key metabolites having a protection effect on sperm motility are screened from 3613 kinds of metabolites and 38 pieces of sperm character analysis of breeding bulls. The function is verified through a standardized freezing process, it is found that after the sperm concentration is adjusted to 6 * 10 < 8 > / mL, and incubation at 37 DEG C, gradient cooling and liquid nitrogen programmed freezing are carried out, it is found through detection that the effect of a treatment group added with 15 [mu] M of 4, 6-dihydroxyquinoline and 5 [mu] M of flavanone is the most remarkable, and the vitality of frozen sperms can be improved. The invention further finds that the 4, 6-dihydroxyquinoline and the flavanone are combined for use, so that a remarkable synergistic interaction effect can be generated. The method is simple and convenient to operate and easy to popularize, and provides a reliable means for cryopreservation of the semen.

This application belongs to the field of food processing technology, and particularly relates to a preparation technology and application of fruit powder products with high citrus flavonoid content. The preparation technology of fruit powder products with high citrus flavonoid content provided by this application first extracts blood orange peel through supercritical CO2 combined with ethanol extraction. The obtained blood orange peel flavonoid extract contains rich polymethoxylated flavonoid active ingredients, flavanones, flavonols, and flavonoid active ingredients. After being added to fruit powder, the types of flavonoids and the total flavonoid content are greatly increased, and the antioxidant and other biological activities are improved, thereby solving the problem of the lack of deep processing technology for whole blood oranges in the prior art.

Various embodiments of the invention utilize flavanones, including, but not limited to, 3-hydroxyflavone compounds and 3-hydroxyflavone sugar compounds, to treat blood, bleeding, and / or bleeding disorders. As described herein, flavanones significantly reduce blood clotting time in normal / hemophilic blood and normal / hemophilic animal models. As also described herein, flavanones reduced blood clotting time and increased clotting efficiency in blood without any apparent risk of immunogenicity or risk of unwanted blood clots. As also described herein, flavanones reduce the inhibitory activity of antithrombin on thrombin-driven blood clotting, thereby increasing the effectiveness of the thrombin mechanism in clotting blood.

The invention relates to the technical field of antibacterial drugs, and particularly discloses a pleuromutilin derivative and application thereof, and the pleuromutilin derivative is structurally characterized in that flavanone or an oximido / oxime ether derivative of flavanone is connected to a side chain of pleuromutilin C14 through mercaptoethylamine, piperazine or an oxygen atom. According to the invention, pleuromutilin is taken as a raw material, flavanone (naringenin and hesperetin) with an antibacterial effect and an oximido / oxime ether derivative thereof are introduced into a pleuromutilin C14 side chain, and the flavanone and the oximido / oxime ether derivative thereof show huge potential as a dominant structural unit due to a specific C6-C3-C6 basic skeleton (C2 and C3 sites are single bonds) and wide antibacterial activity; the flavonoid compound is combined with pleuromutilin to synthesize a plurality of dihydroflavonoid pleuromutilin derivatives with excellent antibacterial activity, and a new choice is provided for designing a new generation of pleuromutilin derivatives through a hybridization strategy, improving the drug characteristics of the pleuromutilin derivatives while improving the activity and anti-infection drugs of the pleuromutilin derivatives.

The application relates to the technical field of medicines, and discloses a kind of isoprenyl-santonin compounds and preparation method and application thereof.The isoprenyl-santonin compound is any one of compounds shown in formula 1 to formula 9, the isoprenyl-santonin compound is isolated and extracted from the secondary metabolites of Aspergillus stellatus, and has excellent antitumor activity and anti-inflammatory activity, can be applied to the preparation of antitumor drugs and anti-inflammatory drugs, and has great practical value.

The invention relates to a method for constructing HPLC characteristic chromatograms of a Yumai decoction reference sample, two sets of HPLC methods are adopted to comprehensively characterize component characteristics of four medicines in a Yumai decoction prescription, the first set of HPLC characteristic chromatogram determines 10 characteristic peaks, and the first set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of prepared rehmannia root, and the second set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of Yumai decoction. The components are respectively catalpol, adenosine, 5-hydroxymethylfurfural, rehmannia D, verbascoside and isoacteoside; the rhizoma anemarrhenae has three characteristic peaks, namely neomangiferin, mangiferin and isomangiferin; the first set of HPLC characteristic chromatogram determines five characteristic peaks which belong to beta-ecdysterone, the second set of HPLC characteristic chromatogram determines five characteristic peaks which belong to five characteristic peaks of radix ophiopogonis, and two peaks of the characteristic peaks are identified to be methylophiopogonanone A and methylophiopogonanone B respectively; the two sets of specific chromatogram methods established by the invention have good performance and better pertinence, can comprehensively characterize the component characteristics of the four medicines in the Yumai decoction prescription, and can effectively monitor the quality of the Yumai decoction preparation.

The application belongs to the technical field of bioengineering, and particularly relates to an AbF3H enzyme and application thereof. Bioinformatics analysis finds that the enzyme belongs to an alpha-ketoglutarate-dependent dioxygenase family, has the potential of catalyzing synthesis of dihydromorin, and an amino acid sequence thereof is shown as SEQ ID No.: 1. Escherichia coli BL21 (DE3) is used as a host bacterium, a plasmid pACYCDuet-1 is used to construct an expression system of the enzyme, the recombinant strain can use naringenin as a substrate, and dihydromorin is synthesized by using a whole cell catalysis method with the aid of vitamin C, ferrous sulfate and alpha-ketoglutarate. The preservation number of the recombinant strain is CCTCC M 20251244. The content of the application enriches a flavanone 3-hydroxylase resource library, and lays a foundation for industrialized production of dihydromorin and other flavonoid compounds.

The application discloses a salt-tolerant endophytic Pantoea and application thereof, and relates to the technical field of microorganisms. Pantoea endophytica A salt-tolerant endophytic Pantoea (TH1) is isolated from grape young fruits, and the preservation number of the strain is CCTCC NO: M 2025960. The strain can promote grape growth, improve grape quality, increase the content of vanillin in the grape, increase the content of anthocyanin, flavonoids and flavanone in the grape, and reduce the content of tannin and total phenol in the grape. In addition, the strain has the abilities of high-efficiency potassium solution, nitrogen fixation, phosphorus dissolution and IAA production, and salt tolerance, can improve plant photosynthesis, improve soil environment and promote plant growth under salt stress, and can also be used for preventing and treating grape powdery mildew.

The invention provides a thin-layer identification method for radix ophiopogonis and rhizoma anemarrhenae in a Yuyu decoction reference sample and application thereof.The radix ophiopogonis and rhizoma anemarrhenae are identified through an established thin-layer chromatography method, and the method mainly comprises the steps that trichloromethane is adopted as an extraction solvent, methylophiopogonanone B is adopted as a reference substance, toluene-methanol (80: 3) is adopted as a developing solvent, and the thin-layer chromatography method is adopted to identify the radix ophiopogonis and rhizoma anemarrhenae; identifying the medicinal flavor of the radix ophiopogonis under the condition of 365nm ultraviolet light; 50% ethanol is used as an extraction solvent, a rhizoma anemarrhenae reference medicinal material, mangiferin and timosaponin BII are used as contrasts, an upper-layer solution of n-butyl alcohol-glacial acetic acid-water (4: 1: 5) is used as a developing solvent, 10% ethanol sulfate is used as a color developing agent, and the medicine flavor of rhizoma anemarrhenae is identified under the condition of 365nm ultraviolet light. The thin-layer chromatography identification method developed by the invention not only has a visual and vivid identification result, but also has good specificity, durability and reproducibility, and perfects a quality control system of the Yumai decoction preparation.

The application belongs to the technical field of pharmaceutical chemistry and pharmacology, and particularly relates to a flavanone schmidt rearrangement derivative, a preparation method and application thereof. A structural formula of the flavanone schmidt rearrangement derivative is shown in the following formula: wherein R represents one of benzyl, propynyl, allyl, isopentenyl, methyl, ethyl, isopropyl and n-butyl. The flavanone schmidt rearrangement derivative is a rearrangement compound obtained by schmidt rearrangement reaction of flavanone, and a derivative obtained by further alkylation. Pharmacological research shows that the compound has strong liver cancer cell proliferation inhibition activity, and can be applied to preparation of a drug for preventing and treating liver cancer.

The invention discloses a preparation method and application of a multi-mode and multifunctional molecular cage high performance liquid chromatography stationary phase. The novel molecular cage RCC3-R bonded silica gel stationary phase is prepared by covalently bonding RCC3-R to the surface of silica gel through a ring-opening chemical reaction of a secondary amino group of RCC3-R and an epoxy group of 3-(2, 3-epoxypropoxy) propyltrimethoxysilane under an alkaline condition. The prepared stationary phase has four separation modes of normal phase, reverse phase, ion exchange and hydrophilic chromatography; the compound shows excellent separation and analysis performance for resolution of chiral compounds (alcohols, naphthol and flavanone) and separation of achiral compounds (benzene naphthenic base homologues, polybenzene rings, phenols, aromatic amines, sulfonamides, acids and nucleosides) with a wide polarity range, and can make up for the defects of single-mode chromatography in complex sample analysis. The method has the remarkable advantages of simple and environment-friendly preparation process, high separation selectivity, good repeatability and the like, and has good popularization and application prospects.

The application provides a silicon-based homoisoflavonoid activated powder and a preparation method and application thereof, and belongs to the auxiliary technical field of medical detection materials. The application takes medicinal grade fumed silica (fumed silica powder) as an inorganic core, takes natural homoisoflavonoid active molecule sappanone A as an organic functional shell, and realizes uniform modification and firm anchoring of the natural active molecule on the surface of the inorganic carrier through stable covalent bonding. In the activated powder, the sappanone A as the shell is derived from a natural medicinal plant, rich in a single-double bond alternating flexible conjugated system capable of free internal rotation in the chemical structure, and has intrinsic color and intramolecular rotation regulation characteristics, and can accurately realize the visual conversion from micro-area stress change to optical signal in various medicinal diagnosis and treatment aid systems such as a gastroscope bubble driving agent, a medicinal catheter lubricating oil agent and the like, and has a congenital functional advantage at a structural level.

The present disclosure generally relates to certain flavanone compounds and the use of such compounds to modify the flavor or taste of an ingestible composition. These flavanone compounds include 8-methylnaringenin (5,7,4'-trihydroxy-8-methylflavanone), 5,3'-dihydroxy-7,4'-dimethoxyflavanone, 5-hydroxy-7,3',4'-trimethoxyflavanone, 5,4'-dihydroxy-7,3'-dimethoxyflavanone, or any combinations thereof. In some embodiments, the use of these flavanone compounds includes their use to enhance a sweet taste, their use to reduce an off taste of a high-intensity sweetener, their use to enhance a salty taste, their use to enhance an umami or kokumi taste, or any combination thereof. In certain embodiments, such compounds are used in combination with other natural taste modifiers, such as flavonoids or dihydrochalcones. In certain aspects, the disclosure provides ingestible compositions that include such flavanone compounds. In some related aspects, the ingestible compositions are, or are included within, various flavored products, such as food products, beverage products, pharmaceutical products, oral care products, or animal feed products.

The invention discloses an aurone derivative of which the structural formula is shown as a formula I, wherein X is selected from one of-OH, C1-6 alkoxy, C1-6 alkylthio,-C6H5 and-CH = CH2; r1 and R2 are one or more substituent groups on respective benzene rings, and R1 and R2 are independently selected from one or more of-H,-NO2,-F,-Cl,-Br,-I, C1-6 alkyl, C1-6 alkoxy, dialkylamino, monoalkylamino,-OH,-NH2,-C6H5 and-CH = CH2. The invention further discloses application of the compound composition of the aurone derivative and the ultraviolet sun-screening agent in full-wave-band light absorption cosmetics. The aurone derivative disclosed by the invention has a good effect, and has a good application prospect when being used as a component of cosmetics and dermatological compositions by compounding the aurone derivative with an ultraviolet sun-screening agent.

The invention discloses application of a flavonoid compound in preparation of a lipid metabolism regulation preparation, and belongs to the technical field of biological medicine. The invention discloses an application of a flavonoid compound in preparation of a lipid metabolism regulation preparation. The flavonoid compound is amentoflavone, herba lycopi flavone, isofulvic alcohol, sub-glycoside chalcone, sophora japonica flavanone G or silver forging glycoside. The flavonoid compound disclosed by the invention can improve diseases related to lipid metabolism by inhibiting lipid accumulation. And the compound has the advantages of good safety, simple preparation process, high synthesis yield and the like, so that the compound has a good application prospect.

The invention belongs to the technical field of cosmetic raw material extraction, and particularly relates to a natural cosmetic raw material extraction process. The natural cosmetic raw material extraction process provided by the invention has the beneficial effect that the extraction efficiency of natural flavanone can be improved. The natural cosmetic raw material extraction process comprises the following steps: S1, crushing extraction raw materials, and putting the crushed extraction raw materials into processing equipment; s2, putting the grinding material into processing equipment, mixing the grinding material with the crushed extraction raw material, and grinding; s3, adding an extracting agent into the processing equipment, mixing the extracting agent with the ground powder, and extracting to obtain an extracting solution; s4, solid residues in the extraction liquid are filtered; s5, decolorizing the filtered extract liquor; s6, decompressing, concentrating and freeze-drying the decolored extract liquor to obtain the natural flavanone. The raw materials extracted in the step S1 are one or more of epicarp of citrus plants such as lemon, citrus and fragrant citrus. The abrasive material in the step S2 is silicon dioxide particles with the particle size of 400-500 meshes.

This invention provides an LC-MS / MS method for the simultaneous detection of multiple major components of Shengmai Yin (Codonopsis pilosula formula) in plasma. The analytes include syringin, schisandrol A, gomisin D, schisandrol B, gomisin G, schisandrin A, schisandrin A, schisandrin B, codonopsis glycoside I, codonopsis alkynyl glycoside, methyl ophiopogon flavanone A, and methyl ophiopogon flavanone B. The method includes the following steps: preparation of a series of standard curve working solutions; preparation of standard curve samples; preparation of quality control working solutions; preparation of quality control samples; preparation of a mixed internal standard working solution; pretreatment of the standard curve, quality control, and analyte plasma samples using protein precipitation; sample detection; and quantitative determination based on the peak area ratio of the analyte to the internal standard to obtain the standard curve. The methodological investigation results of this invention show that the established method meets the requirements for in vivo biological sample determination, the pretreatment process is simple, and the method has good sensitivity, strong specificity, and high specificity, making it suitable for the detection of compounds with low concentrations.

The invention relates to oxygen methyl transferase as well as a coding gene CsOMT5 and application thereof, and belongs to the technical field of gene engineering. The oxygen methyl transferase is obtained through prokaryotic expression of a CsOMT5 gene cloned from rock candy orange peel for the first time. In-vitro enzyme activity experiments show that the oxymethyltransferase can catalyze various flavonoid compounds, such as flavanones, flavonoids, flavanols and flavonols, and the catalytic site is 3 ' / 5' / 5 / 7. Citrus homologous transient overexpression and gene silencing experiments prove that the oxymethyltransferase participates in biosynthesis of citrus PMFs and promotes accumulation of the citrus PMFs, which further proves that the oxymethyltransferase has relatively high research prospects and application values in the aspects of improving the nutritional quality of citrus fruits and industrially producing PMFs.

The present disclosure relates generally to certain flavanone compounds and the use of such compounds to modify the flavor or taste of ingestible compositions. These flavanone compounds include 8-methylnaringenin (5, 7, 4 '-trihydroxy-8-methylflavanone), 5, 3'-dihydroxy-7, 4 '-dimethoxyflavanone, 5-hydroxy-7, 3', 4 '-trimethoxyflavanone, 5, 4'-dihydroxy-7, 3 '-dimethoxyflavanone, or any combination thereof. In some embodiments, the use of these flavanone compounds includes use to enhance sweetness, use to reduce off-taste of high intensity sweeteners, use to enhance salty taste, use to enhance umami or rich taste, or any combination thereof. In certain embodiments, these compounds are used in combination with other natural taste modifiers (e.g., flavonoids or dihydrochalcones). In certain forms, the present disclosure provides ingestible compositions comprising such flavanone compounds. In some related forms, the ingestible composition is a variety of flavored products or is included in a variety of flavored products, such as a food product, a beverage product, a pharmaceutical product, an oral care product, or an animal feed product.

This invention belongs to the fields of medicinal chemistry and pharmacology, specifically relating to a flavanone indole derivative, its preparation method, and its application. The structural formula of the flavanone indole derivative is shown below: where R represents one of 5-methyl, 5-fluoro, 5-bromo, 5-chloro, 4-chloro, 4-bromo, 4-fluoro, and 4-nitro. This flavanone indole derivative is a compound combining the pharmacophores of flavanone and indole. Pharmacological studies have shown that this type of compound possesses strong inhibitory activity against breast cancer cell proliferation and can be used in the preparation of drugs for the prevention and treatment of breast cancer.

Various embodiments of the invention utilize flavanones, including, but not limited to, 3-hydroxyflavone compounds and 3-hydroxyflavone sugar compounds, to treat blood, bleeding, and / or bleeding disorders. As described herein, flavanones significantly reduce blood clotting time in normal / hemophilic blood and normal / hemophilic animal models. As also described herein, flavanones reduced blood clotting time and increased clotting efficiency in blood without any apparent risk of immunogenicity or risk of unwanted blood clots. As also described herein, flavanones reduce the inhibitory activity of antithrombin on thrombin-driven blood clotting, thereby increasing the effectiveness of the thrombin mechanism in clotting blood.