58 results about "Flavanone" patented technology

The invention discloses recombinant saccharomyces cerevisiae, a 11-seed oil fermentation product as well as a preparation method and application of the 11-seed oil fermentation product, and belongs to the technical field of synthetic biology and fermentation product preparation. According to the recombinant saccharomyces cerevisiae disclosed by the invention, a caffeic acid coenzyme A ligase 4CL coding gene, a chalcone isomerase CHI coding gene, a flavanone-3-hydroxylase F3H coding gene and a chalcone synthase CHS mutant coding gene are over-expressed, and the chalcone synthase CHS mutant is preferably Y69H / H71Y / Q161K. The preparation method of the 11-seed oil fermentation product comprises the following steps: fermenting a recombinant saccharomyces cerevisiae fermentation solution in the presence of a sophora flower bud extract and caffeic acid to obtain a first fermentation solution; fermenting the lactic acid bacteria fermentation liquor in the presence of 11 seed oil to obtain second fermentation liquor; and mixing the first fermentation liquid and the second fermentation liquid, continuing fermentation, and respectively collecting an oil phase and a water phase after fermentation is finished, so as to obtain a fermentation product. The product provided by the invention has multivitamin antioxidant activity.

The invention discloses an application of a soybean 2-hydroxyisoflavanone dehydratase gene in improving the growth of intercropping corn. The gene HID1 can promote soybean to synthesize isoflavone, recruit bacillus and promote mycorrhizal fungi infection, so that the growth of intercropped corn is promoted, and the gene HID1 has important significance in illuminating the biological function of the GmHID1 gene in leguminous crop intercropping and regulating and controlling the synergistic growth promotion of plants, mycorrhizal fungi and bacillus in intercropping.

This invention discloses 2'-hydroxy-4,4',6'-trimethoxychalcone and 5,7,4'-trimethoxyflavanone sulfonamide derivatives, their synthesis methods and applications, belonging to the field of pharmaceutical technology, and involving two series of compounds: 3-sulfonamido-2'-hydroxy-4,4',6'-trimethoxychalcone and 8,3'-disulfonamido-5,7,4'-trimethoxyflavanone, and their preparation methods. Starting with 2-hydroxy-4,6-dimethoxyacetophenone and p-anisaldehyde or 4-methoxy-3-nitrobenzaldehyde, 2'-hydroxy-4,4',6'-trimethoxychalcone or 3-amino-2'-hydroxy-4,4',6'-trimethoxychalcone intermediates were synthesized via the Claisen-Schmidt reaction. Then, through sulfonation and intramolecular Michael addition reactions, 2'-hydroxy-4,4',6'-trimethoxychalcone and 5,7,4'-trimethoxyflavanone sulfonamide derivatives were synthesized. The preparation method is simple, mild, and yields high results. In vitro and in vivo activity tests show that the derivatives disclosed in this invention have significant in vitro inhibitory activity against gastric cancer, esophageal cancer, colorectal cancer, and lung cancer cell lines, acting as inhibitors of the protein YBX1 / RPN1. Clinically, they can be applied to targeted therapy for cancers such as gastric cancer, esophageal cancer, colorectal cancer, and lung cancer.

The present invention discloses a method for asymmetric transfer hydrogenation synthesis of chiral flavanol compounds by dynamic kinetic resolution, and the catalyst used is a chiral ruthenium diamine complex. Through this method, asymmetric transfer hydrogenation of flavanone derivatives can be realized, and optically pure flavanol compounds containing three consecutive chiral centers can be obtained in high yield (the enantiomeric excess can reach 99%, and the diastereomeric ratio can reach >20:1). The present invention has high enantioselectivity and diastereoselectivity, is simple and easy to operate, the catalyst is commercially available, the reaction conditions are mild, the energy consumption is low, it is environmentally friendly and has good yield, and gram-scale experiments can be realized without loss of activity and enantioselectivity.

The present disclosure generally relates to 3-alkoxyflavanone compounds and the use of such compounds to impart and / or enhance sweetness, mask bitterness, improve texture, or mask astringency. In certain embodiments, such compounds are used in combination with other flavonoids or dihydrochalcones. In certain aspects, the present disclosure provides ingestible compositions comprising such flavanone compounds. In some related aspects, the ingestible compositions are or are included in various flavored products, such as food, beverage products, pharmaceuticals, or oral care products.

The present disclosure generally relates to certain flavanone compounds and the use of such compounds to modify the flavor or taste of an ingestible composition. These flavanone compounds include 8-methylnaringenin (5,7,4'-trihydroxy-8-methylflavanone), 5,3'-dihydroxy-7,4'-dimethoxyflavanone, 5-hydroxy-7,3',4'-trimethoxyflavanone, 5,4'-dihydroxy-7,3'-dimethoxyflavanone, or any combinations thereof. In some embodiments, the use of these flavanone compounds includes their use to enhance a sweet taste, their use to reduce an off taste of a high-intensity sweetener, their use to enhance a salty taste, their use to enhance an umami or kokumi taste, or any combination thereof. In certain embodiments, such compounds are used in combination with other natural taste modifiers, such as flavonoids or dihydrochalcones. In certain aspects, the disclosure provides ingestible compositions that include such flavanone compounds. In some related aspects, the ingestible compositions are, or are included within, various flavored products, such as food products, beverage products, pharmaceutical products, oral care products, or animal feed products.

Present invention discloses a novel green fluorescence silver nanodots prepared with naringenin, a flavanone, by a near green synthesis without adding harsh reducing agent such as sodium borohydride and others. Particularly, the present invention provides silver nanodots (quantum dot like nanostructure) of average size 3.5 nm. The preparation method is a unique kind of single pot green synthesis method wherein no common and strong reagent like sodium borohydride, ascorbic acid surfactant, thiol containing reagent or protein solutions are used. Alternatively, a simple flavanone, aromatic organic molecule that contains three OH groups is used both as a mild reducing and stabilizing agent for the preparation of the particles at room temperature (25°C). The aqueous suspension of the invented nanodots product is quite stable in room temperature. It produces strong green fluorescence with average fluorescence lifetime ~2.37 ns and produces a large stocks shift.

The invention relates to application of flavanone as a bactericidal synergist in plant disease control. Specifically, the bactericidal synergist, a substituted aniline bactericide, a methoxy acrylate bactericide, a triazole bactericide and a substituted benzene bactericide are jointly applied to prevention and treatment of diseases. Flavanone has a synergistic effect on the bactericide, and can enhance the toxicity of the bactericide, improve the sensitivity of a drug-resistant strain to the bactericide and promote the control effect of the bactericide on indoor or field drug-resistant phytopathogens when being mixed with the bactericide, and can effectively reduce the dosage of the bactericide. And a new method is provided for the development and application of the existing new bactericide preparation.

A composition for forming a protective film for a wet etching solution for semiconductors, comprising: component (A): a film-forming component; component (B): a compound selected from the group consisting of a condensed tannin (b1) obtained by polymerizing a compound having a flavanol skeleton, a hydrolyzable tannin (b2) obtained by ester-bonding a sugar and an aromatic compound of gallic acid or ellagic acid, and a flavonoid (b3) derived from a compound having a flavanone skeleton structure; and component (C): a solvent.

The invention belongs to the technical field of bioengineering, and particularly relates to an AbF3H enzyme and application thereof, it is found through bioinformatics analysis that the enzyme belongs to an alpha-ketoglutaric acid dependent dioxygenase family and has the potential of catalytic synthesis of dihydrokaempferol, and the amino acid sequence of the enzyme is shown as SEQ ID No.1. Escherichia coli BL21 (DE3) is used as host bacteria, plasmid pACYCDuet-1 is used for constructing an expression system of the enzyme, and a recombinant strain can be used for synthesizing dihydrokaempferol by taking naringenin as a substrate and supplementing vitamin C, ferrous sulfate and alpha-ketoglutaric acid through a whole-cell catalysis method. The preservation number of the recombinant strain is CCTCC (China Center For Type Culture Collection) M 20251244. The content of the invention enriches the resource library of flavanone 3-hydroxylase, and lays a foundation for industrial production of dihydrokaempferol and other flavonoid compounds.

The invention discloses a gingko GbF3H gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbF3H gene is obtained by analyzing and screening on the basis of ginkgo transcriptome data in the earlier stage of a research group, the GbF3H gene is flavanone 3-hydroxylase, the nucleotide sequence of the GbF3H gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbF3H gene is shown as SEQ ID NO.2. A GbF3H overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the difference of 28 flavonoid metabolites is obvious, and the contents of 9 flavonoid substances such as formononin are up-regulated. The invention provides a theoretical basis, a valuable gene resource and a method for analyzing a ginkgo flavone synthesis mechanism and improving the flavone yield through metabolic engineering, and has important significance on development and utilization of ginkgo resources and cultivation of new germplasm with medicinal value.

The invention belongs to the technical field of medicinal chemistry and pharmacology, and particularly relates to a flavanone thiazole derivative as well as a preparation method and application thereof. The structural formula of the flavanone thiazole derivative is shown in the specification, and in the structural formula, R represents one of 2-iodophenyl, 2, 4-dichlorophenyl, 4-fluorophenyl, 2-bromobenzyl, 4-bromophenyl, 2-bromophenyl, 4-chlorobenzyl and 4-methylphenyl. The flavanone thiazole derivative is a flavanone thiazole ring-fused derivative, and pharmacological research shows that the compound has high liver cancer cell proliferation inhibition activity and can be applied to preparation of drugs for preventing and treating liver cancer.

This invention discloses the application of cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins in the preparation of drugs for treating or preventing aging and lowering lipids. In vivo experiments have confirmed that the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention, without reproductive toxicity to a *C. elegans* model, can prolong the average lifespan of nematodes, improve their motility, enhance their stress resistance, reduce endogenous ROS levels and lipofuscin content, and decrease the body fat content of nematodes. Therefore, the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention can be used in the preparation of drugs for treating or preventing aging and lowering lipids.

The invention relates to organic chemistry, and more particularly to flavanone derivatives, and even more particularly to derivatives of flavanones modified by a guanidine group. The given compounds can be used in the field of medicine as medicinal preparations. Claimed are compounds having the specified general formula in which R1, R2, R4 is Н or OH, and R3 is OH or ОСН3. The compounds of this general formula, namely flavanone hydrazones, exhibit elevated solubility in water and, therefore, greater bioavailability than the parent flavanones.

This invention discloses a method for preparing flavanone extracts through microbial co-fermentation and its application in lipid-lowering activity, belonging to the field of food nutrition and functional components. By co-fermenting orange peel raw materials with yeast and Monascus purpureus, the synergistic metabolic effects of different microorganisms are utilized to increase the content and reconstruct the composition of flavanones from orange peel. The bioactivity of the fermentation products was evaluated using a mouse 3T3-L1 preadipocyte differentiation model. The results showed that the flavanones from orange peel treated with co-fermentation had a significant inhibitory effect on lipid accumulation, and their lipid-lowering activity was significantly better than that of unfermented or single-strain fermentation products. The method of this invention is simple, reproducible, and suitable for large-scale production, and can be applied to the development of lipid-lowering functional health products or dietary supplements.

The invention discloses an application of a compound or a composition thereof as a semen cryopreservation solution. According to the invention, through whole genome re-sequencing and seminal plasma non-target metabonomics, a regulation mechanism of interaction of genome variation and metabolites on semen traits is analyzed. Key metabolites having a protection effect on sperm motility are screened from 3613 kinds of metabolites and 38 pieces of sperm character analysis of breeding bulls. The function is verified through a standardized freezing process, it is found that after the sperm concentration is adjusted to 6 * 10 < 8 > / mL, and incubation at 37 DEG C, gradient cooling and liquid nitrogen programmed freezing are carried out, it is found through detection that the effect of a treatment group added with 15 [mu] M of 4, 6-dihydroxyquinoline and 5 [mu] M of flavanone is the most remarkable, and the vitality of frozen sperms can be improved. The invention further finds that the 4, 6-dihydroxyquinoline and the flavanone are combined for use, so that a remarkable synergistic interaction effect can be generated. The method is simple and convenient to operate and easy to popularize, and provides a reliable means for cryopreservation of the semen.

To provide a polyphenol composition allowing specific polyphenols, being water-poorly-soluble compounds, to be dissolved at high concentration in a water-based solvent and enabling easy preparation of a highly transparent solution, and a method for producing the same.SOLUTION: The polyphenol composition of the present invention includes a water-poorly-soluble compound and a sucrose fatty acid ester, wherein the water-poorly-soluble compound is one polyphenol selected from the group consisting of flavones, flavanones, isoflavones, and flavonols, and the sucrose fatty acid ester has an average degree of esterification of 1.01 or more and 1.1 or less.SELECTED DRAWING: None

This application belongs to the field of food processing technology, and particularly relates to a preparation technology and application of fruit powder products with high citrus flavonoid content. The preparation technology of fruit powder products with high citrus flavonoid content provided by this application first extracts blood orange peel through supercritical CO2 combined with ethanol extraction. The obtained blood orange peel flavonoid extract contains rich polymethoxylated flavonoid active ingredients, flavanones, flavonols, and flavonoid active ingredients. After being added to fruit powder, the types of flavonoids and the total flavonoid content are greatly increased, and the antioxidant and other biological activities are improved, thereby solving the problem of the lack of deep processing technology for whole blood oranges in the prior art.

The invention belongs to the field of gene engineering, and relates to a gene combination for synthesizing delphinidin, a recombinant plasmid combination, a strain and a method for heterologous biosynthesis of delphinidin. The gene combination comprises the following genes: a flavanone 3-hydroxylase gene TbF3H, a P450 cytochrome oxidase gene TbCYP, a flavanonol 4-reductase TbDFR, an anthocyanin synthetase TbANS and a P450 cytochrome reductase gene LjCPR, and the flavanone 3-hydroxylase gene TbF3H, the P450 cytochrome oxidase gene TbCYP, the flavanonol 4-reductase TbDFR, the anthocyanin synthetase TbANS and the P450 cytochrome reductase gene The invention provides a potential method for replacing traditional artificial cultivation or submerged fermentation acquisition, and lays a foundation for further transforming efficient biosynthesis through metabolic engineering.

Various embodiments of the invention utilize flavanones, including, but not limited to, 3-hydroxyflavone compounds and 3-hydroxyflavone sugar compounds, to treat blood, bleeding, and / or bleeding disorders. As described herein, flavanones significantly reduce blood clotting time in normal / hemophilic blood and normal / hemophilic animal models. As also described herein, flavanones reduced blood clotting time and increased clotting efficiency in blood without any apparent risk of immunogenicity or risk of unwanted blood clots. As also described herein, flavanones reduce the inhibitory activity of antithrombin on thrombin-driven blood clotting, thereby increasing the effectiveness of the thrombin mechanism in clotting blood.

Consumer products are provided. The consumer products include: (a) at least one sweetening agent, and (b) a sweetness modifier comprising an extract or fraction from Viscum articulatum or at least one flavan and / or flavanone compound. The at least one sweetening agent is present in a sweetening amount.

The present invention provides a transgenic plant having enhanced stress resistance and / or with a change in flower color, preferably with a change in flower color to blue, or a self-fertilized or filial generation thereof, or a part of a plant body, a tissue or a cell thereof, and provides a method capable of producing 2-hydroxyflavanone in a simple and efficient manner. The present invention uses a polynucleotide encoding an enzyme belonging to the 2-ketoglutaric acid dependent dioxygenase (ODD) family and having an activity of hydroxylating position 2 of a flavanone.

The invention discloses an application of neoliquiritin in preparation of a medicine for treating myocardial hypertrophy. The invention discovers that the neoliquiritin has a remarkable myocardial hypertrophy resisting effect and has a prospect of being developed into the myocardial hypertrophy resisting medicine. Technicians in the field know that isoliquiritin is an isomeride of neoliquiritin, mother nucleus structures are flavanone skeletons, but the myocardial hypertrophy resistance effect of isoliquiritin is very weak and is far better than that of neoliquiritin, and the technical effect is unexpected by the technicians in the field.

The invention relates to a method for preparing acacetin. In order to overcome the defects that in the prior art, the synthetic process of acacetin is high in cost or plant raw materials are limited, the method for preparing acacetin takes naringenin or hesperetin as a raw material and sequentially comprises the steps of preparing a 2, 4, 6-trihydroxyacetophenone intermediate through hydrolysis, preparing a flavanone intermediate through catalytic closed loop, preparing acacetin through oxidation and the like. The content of acacetin in the prepared final product is greater than 98%, and the method is simple and convenient to operate.

The invention discloses a hydroxyl flavanone derivative as well as a preparation method and application thereof, the hydroxyl flavanone derivative is a compound as shown in a formula I or a formula II, and a synthesis method is provided and comprises the following steps: dissolving chromone 3-formaldehyde, an amine compound and a phenol compound in hexafluoroisopropanol or chlorobenzene; and carrying out microwave reaction at 130 DEG C or reaction in an oil bath at 100 DEG C, and purifying through a silica gel column to obtain a target compound. According to the method, one-pot synthesis is adopted, use of a noble metal catalyst is avoided, and the synthesized hydroxyflavanone derivative has antitumor activity and is used for preparing antitumor drugs.

The invention relates to the technical field of antibacterial drugs, and particularly discloses a pleuromutilin derivative and application thereof, and the pleuromutilin derivative is structurally characterized in that flavanone or an oximido / oxime ether derivative of flavanone is connected to a side chain of pleuromutilin C14 through mercaptoethylamine, piperazine or an oxygen atom. According to the invention, pleuromutilin is taken as a raw material, flavanone (naringenin and hesperetin) with an antibacterial effect and an oximido / oxime ether derivative thereof are introduced into a pleuromutilin C14 side chain, and the flavanone and the oximido / oxime ether derivative thereof show huge potential as a dominant structural unit due to a specific C6-C3-C6 basic skeleton (C2 and C3 sites are single bonds) and wide antibacterial activity; the flavonoid compound is combined with pleuromutilin to synthesize a plurality of dihydroflavonoid pleuromutilin derivatives with excellent antibacterial activity, and a new choice is provided for designing a new generation of pleuromutilin derivatives through a hybridization strategy, improving the drug characteristics of the pleuromutilin derivatives while improving the activity and anti-infection drugs of the pleuromutilin derivatives.

The application relates to the technical field of medicines, and discloses a kind of isoprenyl-santonin compounds and preparation method and application thereof.The isoprenyl-santonin compound is any one of compounds shown in formula 1 to formula 9, the isoprenyl-santonin compound is isolated and extracted from the secondary metabolites of Aspergillus stellatus, and has excellent antitumor activity and anti-inflammatory activity, can be applied to the preparation of antitumor drugs and anti-inflammatory drugs, and has great practical value.

The invention relates to a method for constructing HPLC characteristic chromatograms of a Yumai decoction reference sample, two sets of HPLC methods are adopted to comprehensively characterize component characteristics of four medicines in a Yumai decoction prescription, the first set of HPLC characteristic chromatogram determines 10 characteristic peaks, and the first set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of prepared rehmannia root, and the second set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of Yumai decoction. The components are respectively catalpol, adenosine, 5-hydroxymethylfurfural, rehmannia D, verbascoside and isoacteoside; the rhizoma anemarrhenae has three characteristic peaks, namely neomangiferin, mangiferin and isomangiferin; the first set of HPLC characteristic chromatogram determines five characteristic peaks which belong to beta-ecdysterone, the second set of HPLC characteristic chromatogram determines five characteristic peaks which belong to five characteristic peaks of radix ophiopogonis, and two peaks of the characteristic peaks are identified to be methylophiopogonanone A and methylophiopogonanone B respectively; the two sets of specific chromatogram methods established by the invention have good performance and better pertinence, can comprehensively characterize the component characteristics of the four medicines in the Yumai decoction prescription, and can effectively monitor the quality of the Yumai decoction preparation.

The invention provides a flavanone C3-hydroxylase gene of camptotheca acuminate, a carrier, a protein and application, and belongs to the technical field of bioengineering.CYP71A hydroxylase genes CYP71AU223 and CYP71AU224 in the camptotheca acuminate are obtained through screening, plasmids are constructed and introduced into a saccharomyces cerevisiae WAT11 host cell, the host cell is used for inducing expression of two microsomal proteins of CYP71AU223 and CYP71AU224, and therefore the flavanone C3-hydroxylase gene of the camptotheca acuminate is obtained. The two microsomal proteins both show C-3 site hydroxylation activity on the key intermediate naringenin for flavone biosynthesis, and can be used for preparing the citrus aurantium. According to the invention, molecular docking and site-specific mutagenesis technologies are combined to determine substrate oriented stable key residues of two proteins CYP71AU223 and CYP71AU224, the catalytic activity of the key residues is improved, and the types of hydroxylase at C3 site of flavonoid compounds are enriched.

The invention discloses salt-tolerant endogenous pantoea and application thereof, and relates to the technical field of microorganisms. According to the invention, a strain of endophytic pantoea TH1 is separated from young grape fruits, and the preservation number of the endophytic pantoea TH1 is CCTCC (China Center For Type Culture Collection) NO: M 2025960. The strain can promote the growth of grapes, improve the quality of the grapes, increase the content of vanillin in the grapes, increase the content of anthocyanin, flavonoid and flavanone in the grapes and reduce the content of tannin and total phenols in the grapes. Besides, the strain has the capabilities of efficiently dissolving potassium, fixing nitrogen, dissolving phosphorus and producing IAA (indoleacetic acid) and salt resistance, can improve plant photosynthesis, improve soil environment and promote plant growth under the condition of salt stress, and can also be used for preventing and treating grape powdery mildew at the same time.