54 results about "Delphinidin" patented technology

Personal care composition comprising at least one hair growth regulating compound selected from the group consisting of glyceryl dilaurate, apigenin, tetrahydrocurcumin, oleanolic acid, azelaic acid, sulforaphane, canavanine, pyridoxal 5-phosphate, phytic acid, tannic acid, grape seed extract, NG-nitro-L-arginine-methyl ester, benzamidine, sodium butyrate, betulinic acid, polyornithine, polyarginine, fisetin, jasmonates, methyl-jasmonate, cis-jasmone, caffeic acid phenethyl ester, delphinidin, ethyl abietate, esculetin, sorbic acid methyl ester, canaline, N-formyl-methionine, N-formyl-alanine, taurine, palmitoyl carnitine, undecanol, undecylenic acid, rutin, fusidic acid, phenyl pyruvic acid, L-isoleucine, phenyl glycine, silibinin, silymarin, L-ascorbic acid-6-palmitate, N-undecylenoyl-L-phenylalanine, and salts, derivatives and mixtures of any of the foregoing; and a dermatologically-acceptable carrier.

The invention discloses a method for separating and preparing five high-purity anthocyanidin monomers from grape skins, relating to the preparation of natural pigments and belonging to the fields of food safety and food analytic chemistry. The method comprises the following steps of: firstly, carrying out anthocyanidin digestion on carbernet sauvignon grape skins to obtain crude extractive of anthocyanidin; then purifying the crude extractive by using XAD-7HP macroporous resin, and removing most of moisture absorption components such as saccharides, inorganic salts, phlegmatic temperament and the like in the crude extractive; and carrying out preparative liquid phase separation on the extractive of anthocyanidin subjected to the preliminary purification to obtain the high-purity monomers of the anthocyanidin. The method has the advantages of only using alcohol and acid as a solvent in stages of extraction, purification and separation in the preparation of the anthocyanidin and being environment-friendly, simple, convenient and feasible. The five anthocyanidin monomers obtained after purification are respectively delphinidin glucoside, 3'-methyl delphinidin glucoside, dimethyl delphinidin glucoside, dimethyl delphinidin acetylation glucoside, and dimethyl delphinidin trans-tonka-bean acetylation glucoside, the purities of which are sequentially 99.9%, 99.9%, 99.9%, 99.9%, 91.7%, 95.5%.

The invention relates to an anthocyanin synthesis related protein IbMYB113 as well as a coding gene and application thereof. The amino acid sequence of the IbMYB113 protein is as shown in SEQ ID NO: 2; the nucleotide sequence of the coding gene is shown as SEQ ID NO: 1. According to the invention, IbMYB113 protein and a coding gene thereof are cloned from red sweet potato peel, the IbMYB113 gene is connected to a plant expression vector, a transgenic tobacco plant over-expressing IbMYB113 is obtained through agrobacterium-mediated genetic transformation, pigment accumulation of the whole plant is shown, and the content of delphinidin and cornflower pigment is detected to be obviously increased by high performance liquid chromatography. The IbMYB113 protein and the coding gene thereof provided by the invention have important theoretical significance and application value for promoting synthesis and accumulation of plant anthocyanin.

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.

The invention provides a rose characterized by comprising a flavone and delphinidin added by a genetic modification method. The flavone and delphinidin are typically produced by expression of a transferred flavone synthase gene and flavonoid 3′,5′-hydroxylase gene, respectively. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy (Viola×wittrockiana) flavonoid 3′,5′-hydroxylase gene.

The invention provides an HPLC (High Performance Liquid Chromatography) detection method for simultaneously determining 11 kinds of flavonoids, and belongs to the field of flavonoids detection. The 11kinds of flavonoids are delphinidin glucoside, delphinidin rutinoside, peonidin glucoside, cyanidin rutinoside, cyanidin glucoside, rutin, kaempferol, quercetin, myricetin, isorhamnetin and apigenin.According to the HPLC detection method provided by the invention, different kinds of flavonoids matters can be detected within different retention times through accurate limit on an HPLC condition, the minimum separation degree of a chromatographic peak is 1.54, and simultaneous determination of 11 kinds of flavonoids is realized; data of the embodiment shows that simultaneous determination of 11kinds of flavonoids is realized, and the operation is simple and quick; meanwhile, the standard deviation of the peak area and the retention time is smaller, and higher accuracy is realized.

The invention discloses a blackberry extract. The blackberry extract comprises components in percentage by mass as follows: 58.3%-72.5% of cyanidin-3-glucoside, 11.6%-15.8% of cyanidin-3-xyloside, 9.8%-14.6% of cyanidin-3-oxalyl glucoside, 3.1%-6.4% of cyanidin-3-malonyl glucoside, 2.7%-4.1% of delphinidin-3-xylose and 0.3%-0.8% of cyanidin-3-arabinfuranoside. The extraction steps are as follows: after enzymolysis of blackberry fresh fruits by pepsase and trypsin sequentially, centrifugal separation is performed, a liquid supernatant is obtained and filtered by an ultrafiltration membrane, ingredients with molecular weight larger than 10 kDa are cut off, filtrate is subjected to vacuum freezing drying, and the blackberry extract is obtained. The invention further discloses an application of the blackberry extract in preparation of a liver cell oxidative damage inhibitor.

The invention discloses a delphinidin-3-O-galactoside separation and purification method and an application thereof to preparation of an alpha-glucosidase inhibitor. The separation and purification method takes blueberries with complex anthocyanin compositions as raw materials and includes alcohol extraction concentration, macroporous resin adsorption, preparative liquid phase chromatography purification and high-speed counter-current chromatography separation, preparative liquid phase chromatography and high-speed counter-current chromatography technologies are combined, process parameters are optimized, a high-purity delphinidin-3-O-galactoside monomer is prepared by separation, and the purity of the monomer reaches up to 99%. Activity tests discover that the delphinidin-3-O-galactosidemonomer can remarkably inhibit activity of alpha-glucosidase and can be used for preparing the alpha-glucosidase inhibitor.

This invention provides a prophylactic and therapeutic agent for dry eye, having new ingredients to reduce the deterioration of the lacrimal secretory ability and to inhibit the generation of radical oxygen in lacrimal gland tissue. The prophylactic and therapeutic agent for dry eye of this invention contains the maqui berry extract as the active substance of this invention, i.e. containing delphinidin glycoside extracted from the maqui berry and at least one or more of the active substances delphinidin-3-sambubioside-5-glucoside, delphinidin-3,5-diglucoside, delphinidin-3-sambubioside and delphinidin-3-glucoside, preferably delphinidin-3,5-diglucoside.

The invention belongs to the field of plant cultivation, and particularly relates to a method special for increasing the content of anthocyanin in roselle. In order to increasing the content of the anthocyanin in the roselle, a composition is supplied for the glucoside synthesizing process of delphinidin and cyaniding in the roselle, all the components in the composition serve as precursors and intermediate products which are necessary in biosynthesis of the roselle anthocyanin, and through continuous exploration and improvement, the best using time and applying method of the composition are determined. It is determined that the content of the anthocyanin in roselle calyxes can be increased by multiple times through the method, and the content of the anthocyanin in the calyxes is 121%-293% of that of a control group; in addition, no pollution is generated to the environment, and higher-quality raw materials can be supplied to anthocyanin processing industry through the method.

Disclosed are: a method for producing a chrysanthemum plant having delphinidin-containing petals using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene; and a chrysanthemum plant, a progeny or a vegetative proliferation product of the plant, or a part or a tissue of the plant, the progeny or the vegetative proliferation product, and particularly a petal or a cut flower of the plant. In the method for producing a chrysanthemum plant having delphinidin-containing petals, a flavonoid 3′,5′-hydroxylase (F3′5′H) is caused to be expressed in a chrysanthemum plant using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene.

Disclosed is a rose characterized by containing a flavone and a delphinidin by a genetic recombination technique. Typically, the flavone and the delphinidin are produced by the expression of a flavonesynthesis enzyme gene and a flavonoid 3',5'-hydroxylase gene, respectively, both introduced into the rose. The flavone synthesis enzyme gene may be one originated from a plant belonging to the familyScrophulariaceae, such as one originated from common snapdragon (Scrophulariaceae, Antirrhinum majus) and one originated from torenia (Scrophulariaceae, Torenia hybrida). The flavonoid 3',5'-hydroxylase gene may be one originated from pansy (Viola x wittrockiana).

Provided is: a transformed plant that has blue flower color; a self-fertilized or cross-fertilized progeny of the transformed plant; or a propagule, a portion of a plant body, tissue, or cells of thetransformed plant or the self-fertilized or cross-fertilized progeny of the transformed plant. The petals of the present invention co-express delphinidin anthocyanin and a flavone C-glycoside.

This invention provides a prophylactic and therapeutic agent for dry eye, having new ingredients to reduce the deterioration of the lacrimal secretory ability and to inhibit the generation of radical oxygen in lacrimal gland tissue. The prophylactic and therapeutic agent for dry eye of this invention contains the maqui berry extract as the active substance of this invention, i.e. containing delphinidin glycoside extracted from the maqui berry and at least one or more of the active substances delphinidin-3-sambubioside-5-glucoside, delphinidin-3,5-diglucoside, delphinidin-3-sambubioside and delphinidin-3-glucoside, preferably delphinidin-3,5-diglucoside.

The invention discloses a method for separating and preparing five high-purity anthocyanidin monomers from grape skins, relating to the preparation of natural pigments and belonging to the fields of food safety and food analytic chemistry. The method comprises the following steps of: firstly, carrying out anthocyanidin digestion on carbernet sauvignon grape skins to obtain crude extractive of anthocyanidin; then purifying the crude extractive by using XAD-7HP macroporous resin, and removing most of moisture absorption components such as saccharides, inorganic salts, phlegmatic temperament and the like in the crude extractive; and carrying out preparative liquid phase separation on the extractive of anthocyanidin subjected to the preliminary purification to obtain the high-purity monomers of the anthocyanidin. The method has the advantages of only using alcohol and acid as a solvent in stages of extraction, purification and separation in the preparation of the anthocyanidin and being environment-friendly, simple, convenient and feasible. The five anthocyanidin monomers obtained after purification are respectively delphinidin glucoside, 3'-methyl delphinidin glucoside, dimethyl delphinidin glucoside, dimethyl delphinidin acetylation glucoside, and dimethyl delphinidin trans-tonka-bean acetylation glucoside, the purities of which are sequentially 99.9%, 99.9%, 99.9%, 99.9%, 91.7%, 95.5%.

The invention discloses a symplocos paniculata fruit compound high-speed countercurrent chromatography preparation method which comprises steps of extract preparation, concentration, primary purification, symplocos paniculata fruit anthocyanin separation, symplocos paniculata fruit flavone separation, and the like, and four compounds can be prepared through separation. Results of structure identification show that one of the four compounds is an anthocyanin compound, specifically delphinidin-3-O-elderberry bioside; the four compounds further comprise three flavone compounds, specifically rutin, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside. The product prepared by using the method through separation is high in purity, the flavone compound is relatively good in bioactivity, and basesfor preparation of standard flavone products and further development and utilization of symplocos paniculata fruits are made according to research results.

Provided is an antiseptic swill water fertilizer. Swill is kitchen garbage of restaurants, canteens and families, and quick lime powder, nandina domestica powder, oleander powder, delphinium grandiflorum powder, crinum asiaticum powder, castor powder, poinsettia powder and the like are added into the swill. Crops such as growing vegetables and flowers can be watered with the swill mixed solution, pests can be killed. The swill mixed solution is good in insecticidal effect, the growth condition of plants irrigated by the swill mixed solution is good, the growth of the plants can be promoted, and the swill mixed solution is pollution-free and non-toxic, and plays an important role in protecting the environment. The swill plays an important role in killing and controlling insects, and the mixed solution obtained by adding quick lime powder, nandina domestica powder, oleander powder, delphinidin powder, crinum asiaticum powder, castor powder and poinsettia powder into the swill has a very good insect killing effect. The Antiseptic swill water fertilizer prepared by the invention not only can play a role in preventing and controlling insects and reducing environmental pollution, but also can be used for irrigating plants such as vegetables, fruit trees and flowers as a fertilizer to promote the growth of the plants.

The invention discloses a composition with an effect of reducing blood pressure. The composition comprises the following raw materials, by weight: 5 to 15 parts of soybean peptide, 1 to 3 parts of raspberry extract, 1 to 3 parts of rhodiola rosea extract, 1 to 3 parts of rhizoma polygonati extract, 1 to 3 parts of grape seed extract, 1 to 3 parts of green tea extract, 1 to 3 parts of horseradish tree leaf extract, 0.5 to 2 parts of theanine, 0.5 to 2 parts of astaxanthin, 0.5 to 2 parts of lycopene, 0.5 to 2 parts of resveratrol, 0.5 to 2 parts of curcumin, 0.1 to 1 part of EPA, 0.1 to 1 partof DHA, 0.1 to 1 part of cyaniding, and 0.1 to 1 part of delphinidin. Therefore, the synergistic interaction effect can be realized in the aspect of reducing the blood pressure and the blood pressureof a hypertension patient is effectively reduced, so that the blood pressure of the hypertension patient is kept in a stable state for long time; the synergistic interaction of reducing the cholesterol and blood fat and improving the antioxidant function can be realized; the blood vessel function is improved; the organism immunity is enhanced; substances that are deposited in blood vessels and capable of blocking the blood vessels are removed; and the occurrence and development of hypertension are relieved and improved.

The invention provides a method for preparing tea tree purple bud anthocyanin high-purity products through separation. The method comprises the following steps: extracting tea tree purple buds by an acidic ethanol solution, performing extraction to defat, performing strong acid type action exchange resin separation to remove impurities, performing high-speed countercurrent chromatographic separation to obtain anthocyanin refined products, and performing purification by preparation type high performance liquid chromatography to obtain five anthocyanin high-purity products. According to the method, the fresh leaves of the purple bud tea tree varieties (Zijuan, red bud fingered citron, An 73 and optional 9803) rich in anthocyanin as raw materials and multi-stage separation and purification process technologies are creatively applied; meanwhile, the five tea tree purple bud anthocyanin high-purity products with single anthocyanin purity being more than 90 percent are prepared, wherein thepurity of delphinidin-3-O-(6-(E)p-coumaric acid)pyran galactoside exceeds 98 percent and the monomer quality requirement is met. The whole production process is simple, low in production cost and highin operability; high-quality raw materials are provided for research on pharmacology and medical efficacy and development of functional terminal products; furthermore, high-value utilization of the tea tree purple bud resources is realized truly.

The invention relates to a composition comprising a complex of delphinidin and a sulfoalkyl ether β-cyclodextrin for use as an antiphlogistic and / or immunosuppressive.

The invention discloses a saccharomyces cerevisiae strain and a Wickerhansenula anomala strain, and applications of the saccharomyces cerevisiae strain and the Wickerhansenula anomala strain in improvement of color stability of fruit wine. The two strains of saccharomyces cerevisiae are preserved in the China General Microbiological Culture Collection Center, the preservation number of the saccharomyces cerevisiae is CGMCC (China General Microbiological Culture Collection Center) No.22751, and the preservation number of the Wickerhasenula anomala is CGMCC No.22753. Through detection, the two yeasts have excellent hydroxycinnamic acid decarboxylase activity; the grape wine, the blueberry wine, the mulberry wine and the cherry wine which are rich in anthocyanin such as cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, malvidin-3-O-glucoside, pelargonidin-3-O-glucoside, delphinidin-3-O-glucoside and the like can be added; the content of pyran-type anthocyanin derivatives in the fermentation and ageing process of fruit wine such as blueberry wine is increased, so that the color stability of the aged fruit wine is enhanced, and the sensory quality is improved.

The invention discloses an application of a natural eutectic solvent in selective extraction of anthocyanin, which comprises the following steps: an aqueous solution of the natural eutectic solvent isuniformly mixed with blueberry pomace powder, and an anthocyanin crude extract is extracted under ultrasonic and water bath heating conditions; the natural eutectic solvent takes choline chloride asa hydrogen bond acceptor and oxalic acid as a hydrogen bond donor; wherein the molar ratio of choline chloride to oxalic acid is 1: 0.5-2; wherein in the aqueous solution of the natural eutectic solvent, the mass ratio of water to the natural eutectic solvent is 10-50%; the anthocyanin with delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside as main components canbe obtained from the anthocyanin crude extract. The invention also discloses an application of the natural eutectic solvent in anthocyanin preservation. The eutectic solvent provided by the inventionhas strong selectivity on delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside, and can effectively improve the stability of delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside.

The invention relates to a roselle walnut beverage. The roselle walnut beverage comprises roselle dry calyx juice, walnut juice, common salt, water, sodium cyclamate and white granulated sugar, is tasty salty and sweet with a walnut taste, contains ingredients, such as amino acid, anthocyanin, citric acid, hibiscus acid, delphinidin-3-erberry diglycoside, a plurality of vitamins, iron, calcium, and the like and has a certain healthcare function. Detected by relative departments, the invention has various sanitary indexes according with relative national standards.

The invention belongs to the field of plant cultivation, and particularly relates to a method special for increasing the content of anthocyanin in roselle. In order to increasing the content of the anthocyanin in the roselle, a composition is supplied for the glucoside synthesizing process of delphinidin and cyaniding in the roselle, all the components in the composition serve as precursors and intermediate products which are necessary in biosynthesis of the roselle anthocyanin, and through continuous exploration and improvement, the best using time and applying method of the composition are determined. It is determined that the content of the anthocyanin in roselle calyxes can be increased by multiple times through the method, and the content of the anthocyanin in the calyxes is 121%-293% of that of a control group; in addition, no pollution is generated to the environment, and higher-quality raw materials can be supplied to anthocyanin processing industry through the method.

The invention discloses a method for extracting and separating plant anthocyanin, which comprises the following steps: extracting plants containing anthocyanin by using an acidified ethanol solution, extracting an extracting solution containing the anthocyanin by using ethyl acetate, purifying the extracting solution by strong cation exchange, and then separating an anthocyanin monomer by high pressure liquid chromatography. According to the method for extracting and separating the anthocyanin disclosed by the invention, flavonoid impurities in the anthocyanin extract are effectively removed; according to the present invention, the four anthocyanin monomers (cyanidin-3-O-glucoside, cyanidin-3-O-moruboside, delphinidin-3-O-glucoside, and delphinidin-3-O-moruboside) can be further successfully separated, and the concentration of each anthocyanin monomer is 97-100%;

The invention discloses a VlbZIP30 gene for promoting synthesis of grape anthocyanin and application of the VlbZIP30 gene. The full length of a complete open reading frame sequence of the gene is 978bp, and 325 amino acids are encoded. The method comprises the following steps: constructing a pCAMBIA2300-35s-3 * Flag-VlbZIP30 overexpression vector, and stably transferring the pCAMBIA2300-35s-3 * Flag-VlbZIP30 overexpression vector into Thomson'seedless white 'grapes through an agrobacterium-mediated grape genetic transformation method. The result shows that the content of anthocyanin in leaves and stems of an overexpressed transgenic plant is remarkably increased, and the gene is mainly used for promoting the expression of cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, paeonin-3-O-glucoside and morning glorybin-3-O-glucoside by regulating and controlling the expression of a key gene for biosynthesis of the anthocyanin, so that the yield of the anthocyanin is increased, and the yield of the anthocyanin is increased. The synthesis of anthocyanin such as malvidin-3-O-glucoside improves the accumulation of anthocyanin in plants, thereby promoting the leaves and stems of the white grape variety to turn red from green.

The present invention relates to the application of delphinidin or its salts, and the delphinidin or its salts are aimed at the following bacteria: Staphylococcus aureus showing resistance to antibiotics and aureus showing low sensitivity to antibiotics Staphylococcus aureus, and can at least partially neutralize said bacteria. In addition, the present invention also relates to a method of treating individuals with delphinidin or its salts.

The purpose of the present invention is to provide a breeding method for a plant having a blue flower color with a simpler blue color expression controlling technique, without requiring complex blue color expression mechanisms that have been previously presented and techniques reproducing such mechanisms. A delphinidin-type anthocyanin, in which the 3' and 5' positions of the anthocyanine B ring have been glycosylated, and a flavone glycoside or a flavonol glycoside as a copigment are made to coexist in the cells of a flower organ such as a petal of a plant.

Provided is a method for producing lilies containing a blue pigment (delphinidin) in the petals thereof by introducing a foreign gene into lilies. The method pertaining to the present invention is a method for producing lilies containing delphinidin in the petals thereof, including the following steps: introducing, into a lily, a F3'5'H gene derived from a campanula and comprising a nucleotide sequence encoding a peptide having flavonoid 3'5'-hydroxylase (F3'5'H) activity, such as a nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 11; while also introducing a F3'H gene fragment derived from a lily and comprising a nucleotide sequence encoding a peptide having flavonoid 3'-hydroxylase activity, such as a nucleotide sequence represented by SEQ ID NO: 3 or SEQ ID NO: 16; and inhibiting the expression of endogenous F3'H expression, which acts on cyanidin synthesis in lily petals, while the F3'5'H gene that has been introduced acts to cause the synthesis of delphinidin.