19 results about "Delphinidin" patented technology

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

The invention relates to a CllaF3 '5' H gene and application thereof. The CllaF3 '5' H gene comprises a nucleotide sequence as shown in SEQ ID NO: 1. The CllaF3 '5' H protein comprises a protein coded by the CllaF3 '5' H gene. According to the invention, a CllaF3 '5' H gene is screened and cloned from clematis lanceolata, and the correlation between the CllaF3 '5' H gene and the content of flavonoid metabolites such as delphinidin is found through research; stable germplasm with blue flower potential can be formed by utilizing the gene.

The present invention is focused on extending the lifespan and the healthy period of life (healthspan), delaying the development of chronic diseases and dysfunctions, which is a growing need of aging societies. Among the strategies to promote and extend healthspan, nutrition and food ingredients play a central role. The present invention is related to standardized delphinidin-rich maqui berry composition comprising different amounts and combination of delphinidins, which represent a potent class of antioxidant anthocyanins as agent for reducing progressive loss of skeletal muscle, increasing muscle mass and anti-aging agent or geroprotective agent, and thereby, enhancing the healthspan and preserving locomotive functions for longer periods of life. Such effect was measured as climbing ability during life time in an animal model (Drosophila melanogaster), Experimental assays demonstrated that delphinidin-rich standardized maqui berry extracts upregulate the expression of antioxidant enzymes and mitochondrial homeostasis-related proteins, which determine contractile muscle function. Such delphinidin-rich standardized maqui berry composition is useful as antioxidant supplement for adults and elderly.

The invention provides application of delphinidin-3-glucoside in preparation of uric acid reducing drugs or functional products, and belongs to the technical field of medical application. According to the invention, a novel component-target-metabolic pathway (C-T-M) network is constructed, and the network provides a comprehensive method for analyzing a specific component target and the effect of the specific component target in influencing the metabolic pathway of the HUA. Molecular dynamics (MD) simulation and surface plasmon resonance (SPR) technologies are adopted, and in-vitro and in-vivo metabonomics researches are carried out. The invention discloses a uric acid reducing effect of Dp-3G in red kidney beans, and the uric acid reducing effect is realized by combining with a flavin adenine dinucleotide (FAD) site of xanthine oxidase (XO) enzyme. Therefore, the research provides a new insight for preventing and treating the HUA by specific beans and key natural medicines.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

According to the method disclosed by the invention, the contents of cyanidin-3-O-glucoside, delphinidin-3-O-glucoside and quercetin-3-glucoside in the fresh lonicera caerulea fruits are extracted and determined by applying a ternary eutectic solvent and combining a microwave extraction method. The monomers extracted by the method disclosed by the invention are relatively high in content and very stable. A single factor test carries out orthogonal optimization on selection of the type, the molar ratio, the water content, the solid-to-liquid ratio, the microwave extraction temperature and the microwave extraction time of the ternary eutectic solvent, and an orthogonal test shows that when betaine-glycerol-glucose (Bet-Gly-Glu) with the molar ratio of 1: 3: 1, the water content is 40%, the liquid-to-solid ratio is 20: 1, the extraction temperature is 50 DEG C and the extraction time is 15 min, the content of the glycine betaine is 40%, and the content of the glycine betaine is 40%. The content of the extracted cyanidin-3-O-glucoside is the highest, and the content of the extracted cyanidin-3-O-glucoside is 12.30 mg / g to 12.35 mg / g; the content of the delphinidin-3-O-glucoside is 12.60 mg / g to 12.65 mg / g; the content of the quercetin-3-glucoside is 0.55 mg / g to 0.60 mg / g. The extracted fresh lonicera caerulea fruit pulp extracting solution has relatively high antioxidant and hypoglycemic activity, and can be applied to the fields of antioxidant and hypoglycemic.

The invention relates to the field of pigments, and discloses a dimethyldelphinidin-3-O-glucoside derivative as well as a preparation method and application thereof. The derivative has a chemical structure as shown in a formula 1. The derivative has the beneficial effects of food color generation, color stability and the like, the preparation method provides specific synthesis conditions of the bis-catechol-based pyran type dimethyldelphinidin-3-O-glucoside derivative, and the synthesis conditions comprise the concentration of the raw material dimethyldelphinidin-3-O-glucoside in a reaction system, the reaction temperature, the reaction pH value and the like. The maximum conversion rate of the raw material dimethyldelphinidin-3-O-glucoside can reach 5.41%, and the time for the reaction to reach the maximum conversion rate is shortened as far as possible (can be shortened to about 10 days). # imgabs0 #

The invention belongs to the field of gene engineering, and relates to a gene combination for synthesizing delphinidin, a recombinant plasmid combination, a strain and a method for heterologous biosynthesis of delphinidin. The gene combination comprises the following genes: a flavanone 3-hydroxylase gene TbF3H, a P450 cytochrome oxidase gene TbCYP, a flavanonol 4-reductase TbDFR, an anthocyanin synthetase TbANS and a P450 cytochrome reductase gene LjCPR, and the flavanone 3-hydroxylase gene TbF3H, the P450 cytochrome oxidase gene TbCYP, the flavanonol 4-reductase TbDFR, the anthocyanin synthetase TbANS and the P450 cytochrome reductase gene The invention provides a potential method for replacing traditional artificial cultivation or submerged fermentation acquisition, and lays a foundation for further transforming efficient biosynthesis through metabolic engineering.

The invention discloses an eggplant flavonoid 3 ', 5'-hydroxylase gene and an application method thereof, and relates to the field of biochemistry, the gene sequence is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2; the invention discloses a cloning method of the gene and a gene expression detection method. The gene is applied to creation of new eggplant varieties with different colors, creation of a rare blue system, creation of fruits rich in antioxidant delphinidin anthocyanin, production of natural delphinidin and production of delphinidin through fermentation engineering. According to the invention, on the basis of DNA high-fidelity polymerase, the CDS sequence of the eggplant SmF3 '5' H gene is cloned by using a PCR technology, Sanger sequencing verification is completed, and gene expression quantity detection is carried out. The eggplant SmF3 '5' H gene has huge application potential in the aspects of crop appearance and nutritional quality improvement.

The invention provides a process of producing anthocyanins, including a process of producing delphinidin 3-O-[2-O-(6-O-p-coumaroyl)-glucosyl-6-O-p-coumaroyl] glucoside from delphinidin 3-O-(2-O-glucosyl-6-O-p-coumaroyl) glucoside. The invention also provides processes for the production of delphinidin 3-O-[2-O-(6-O-p-coumaroyl-glucosyl)- 6-O-p-coumaroyl] glucoside 5-O-(6-O-malonyl)-glucoside. Enzymes allowing production of these compounds and genes encoding the enzymes are also provided. The invention provides a process of producing pelargonidin- or cyanidin 3-O-[2-O-(6-O-p-coumaroyl)- glucosyl-6-O-p-coumaroyl] glucoside from pelargonidin- or cyanidin 3-O-(2-O-glucosyl-6-O- p-coumaroyl) glucoside, respectively. The invention also provides processes for the production of pelargonidin- or cyanidin 3-O-[2-O-(6-O-p-coumaroyl-glucosyl)-6-O-p- coumaroyl] glucoside 5-O-(6-O-malonyl)-glucoside. Enzymes allowing production of these compounds and genes encoding the enzymes are also provided. Further intermediates and products of such reactions are provided.

The application discloses a method for rapidly detecting 30 compounds in tea, such as catechins, flavonoids, anthocyanins, phenolic acids and amino acids, wherein the 30 compounds are gallic acid, theobromine, GC, protocatechuic acid, theophylline, EGC, theanine, chlorogenic acid, caffeine, catechin, syringic acid, caffeic acid, vanillic acid, epicatechin, EGCG, delphinidin, salicylic acid, GCG, p-coumaric acid, cyanidin, ferulic acid, sinapic acid, rutin, ECG, pelargonidin, myricetin, theaflavin, quercetin, apigenin and kaempferol. The application can realize simultaneous rapid qualitative and quantitative detection of the 30 compounds, and provides technical support for development of active substances in tea.

The present invention relates to the field of medical technology, and discloses a novel chalcogenin-type diterpene alkaloid compound and its preparation method and application. 20 The delphinidin-type diterpenoid alkaloids carmaloidline C, carmaloidline D, and aconialoidline C all exhibit significant analgesic effects. Carmaloidline C exhibits the strongest analgesic activity, surpassing the positive-acting drug morphine and significantly outperforming the positive-acting drug ibuprofen. Carmaloidline D and aconialoidline C exhibited greater analgesic activity than the positive-acting drug ibuprofen. These drugs could be used to develop analgesics for various acute and chronic pain conditions.

According to the invention, a ternary eutectic solvent is combined with a microwave extraction method to extract anthocyanin components in red beans and cowberries. And the cyanidin-3-O-glucoside and the delphinidin-3-O-glucoside, which are relatively high in content, in the vaccinium ormosia are quantified by using an ultra-high performance liquid chromatograph. The contents of the cyanidin-3-O-glucoside and the delphinidin-3-O-glucoside extracted by the method disclosed by the invention are relatively high and are very stable. The selection of the type, molar ratio, water content, solid-liquid ratio, extraction temperature and extraction time of the eutectic solvent is optimized by a single factor test, and the content of choline chloride-citric acid-1, 4Bu in the molar ratio of 1: 2: 2 is obtained by an orthogonal test when the water content is 15%, the solid-liquid ratio is 1: 30, the extraction temperature is 50 DEG C and the extraction time is 25 minutes, and the content of the choline chloride-citric acid-1, 4Bu in the choline chloride-citric acid-1, 4Bu in the choline chloride-citric acid-1, 4Bu in the choline chloride-citric acid-1, 4Bu is 5%. The highest content of the extracted cyanidin-3-O-glucoside ranges from 1.97 mg / g to 2.8 mg / g, and the highest content of the extracted delphinidin-3-O-glucoside ranges from 5.93 mg / g to 6.8 mg / g. And the extracted red bean and bilberry anthocyanin extracting solution has relatively high antioxidant activity and can be applied to the field of oxidation resistance.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus reuteri XS82 and application thereof in preparation of gastrodia elata leavening with anti-inflammation and high in-vivo and in-vitro bioavailability. The gastrodia elata fermented product prepared by fermenting the lactobacillus reuteri mucus XS82 has high-content gastrodin and parishin E and has antioxidant and anti-inflammatory effects, in-vitro digestion experiments verify that the bioavailability of p-hydroxybenzyl alcohol is remarkably improved, and rat experiments verify that the bioavailability of gastrodin can be remarkably improved, so that the gastrodia elata fermented product can be used for preparing the medicine for treating gastrodia elata. Moreover, the content of effective components such as cockscomb lactone C, 18-deoxycyanidin, caffeoyl feruloyl spermidine, dogwood glycoside and the like in serum can be increased, and the improvement of anti-inflammatory and antioxidant functions is promoted.

The application discloses application of a SlAN2-like gene in regulating content of delphinidin-3-glucoside in tomatoes, a nucleotide sequence of the SlAN2-like gene is shown as SEQ ID NO:1, and overexpression of the SlAN2-like gene can increase the content of delphinidin-3-glucoside in the tomatoes. Compared with the prior art, the application finds that overexpression of the SlAN2-like gene can increase the content of delphinidin-3-glucoside in the tomatoes, and only one anthocyanin of delphinidin-3-glucoside is produced. Therefore, the SlAN2-like gene can be used to produce a tomato material containing only delphinidin-3-glucoside monomers, because anthocyanins in most plants are in the form of a mixture, and extraction and purification work is needed to obtain monomer anthocyanin substances. The created tomato material can be used to reduce technical barriers and costs of extracting delphinidin-3-glucoside.

An example of a composition for promoting metabolic health can include a combination of cyanidin and delphinidin in an amount sufficient to treat metabolic disorders.Furthermore, an example of a method for treating a condition or disorder related to metabolic health in a subject can include reducing endotoxin levels compared to baseline endotoxin levels, and adjusting cardiometabolic biomarkers related to lipid or glucose metabolism from abnormal cardiometabolic baseline levels to normal levels.Exemplary methods can also include reducing liver inflammation, treating high-fat diet-induced colon dysfunction, reducing the expression of toll-like receptors TLR4 and TLR2, reducing plasma endotoxin levels, reducing the expression of NADPH oxidase NOX1, and increasing tight junction proteins in the colon of a subject.

The present application belongs to the technical field of plant extracts, and particularly relates to an antioxidant plant extract composition, which is prepared by mixing the following raw materials in parts by mass: Passiflora edulis fruit extract 30-40 parts, blackcurrant fruit extract 25-35 parts, and Ampelopsis megalophylla leaf extract 20-30 parts. The present application first compounds three plant extracts of Passiflora edulis fruit, blackcurrant fruit and Ampelopsis megalophylla leaf, which can enrich the types of related products. Moreover, Passiflora edulis fruit is rich in flavones such as apigenin and luteolin, blackcurrant fruit is rich in anthocyanins such as cyanidin and delphinidin, and Ampelopsis megalophylla leaf is rich in flavonols such as dihydromyricetin. The synergistic effect of the three extracts can scavenge mitochondrial ROS, protect mitochondrial membrane potential, and improve the activity of respiratory chain complex enzymes, and has good mitochondrial protection and antioxidant damage repair effects, and has important application value for the protection of mitochondrial oxidative damage in the middle-aged and elderly population.

The invention belongs to the technical field of biology, and provides an Indel molecular marker related to delphinidin synthesis capability in broccoli, and the nucleotide sequence composition of the Indel molecular marker is shown as SEQ ID NO: 1 and SEQ ID NO: 2. The invention also provides a primer group and a kit for identification / auxiliary identification of the Indel molecular marker related to the delphinidin synthesis capability in broccoli, and a method for identification / auxiliary identification of whether broccoli has the delphinidin synthesis capability. The Indel molecular marker provided by the invention is used for detecting a broccoli germplasm material or a field breeding material, and a specific single plant with delphinidin synthesis capability can be quickly screened in a seedling stage, so that the workload of field planting and breeding is reduced, and the cultivation progress and accuracy of high-quality characteristic broccoli are improved.