7 results about "Delphinidin" patented technology

According to the method disclosed by the invention, the contents of cyanidin-3-O-glucoside, delphinidin-3-O-glucoside and quercetin-3-glucoside in the fresh lonicera caerulea fruits are extracted and determined by applying a ternary eutectic solvent and combining a microwave extraction method. The monomers extracted by the method disclosed by the invention are relatively high in content and very stable. A single factor test carries out orthogonal optimization on selection of the type, the molar ratio, the water content, the solid-to-liquid ratio, the microwave extraction temperature and the microwave extraction time of the ternary eutectic solvent, and an orthogonal test shows that when betaine-glycerol-glucose (Bet-Gly-Glu) with the molar ratio of 1: 3: 1, the water content is 40%, the liquid-to-solid ratio is 20: 1, the extraction temperature is 50 DEG C and the extraction time is 15 min, the content of the glycine betaine is 40%, and the content of the glycine betaine is 40%. The content of the extracted cyanidin-3-O-glucoside is the highest, and the content of the extracted cyanidin-3-O-glucoside is 12.30 mg / g to 12.35 mg / g; the content of the delphinidin-3-O-glucoside is 12.60 mg / g to 12.65 mg / g; the content of the quercetin-3-glucoside is 0.55 mg / g to 0.60 mg / g. The extracted fresh lonicera caerulea fruit pulp extracting solution has relatively high antioxidant and hypoglycemic activity, and can be applied to the fields of antioxidant and hypoglycemic.

The invention provides a process of producing anthocyanins, including a process of producing delphinidin 3-O-[2-O-(6-O-p-coumaroyl)-glucosyl-6-O-p-coumaroyl] glucoside from delphinidin 3-O-(2-O-glucosyl-6-O-p-coumaroyl) glucoside. The invention also provides processes for the production of delphinidin 3-O-[2-O-(6-O-p-coumaroyl-glucosyl)- 6-O-p-coumaroyl] glucoside 5-O-(6-O-malonyl)-glucoside. Enzymes allowing production of these compounds and genes encoding the enzymes are also provided. The invention provides a process of producing pelargonidin- or cyanidin 3-O-[2-O-(6-O-p-coumaroyl)- glucosyl-6-O-p-coumaroyl] glucoside from pelargonidin- or cyanidin 3-O-(2-O-glucosyl-6-O- p-coumaroyl) glucoside, respectively. The invention also provides processes for the production of pelargonidin- or cyanidin 3-O-[2-O-(6-O-p-coumaroyl-glucosyl)-6-O-p- coumaroyl] glucoside 5-O-(6-O-malonyl)-glucoside. Enzymes allowing production of these compounds and genes encoding the enzymes are also provided. Further intermediates and products of such reactions are provided.

The application discloses a method for rapidly detecting 30 compounds in tea, such as catechins, flavonoids, anthocyanins, phenolic acids and amino acids, wherein the 30 compounds are gallic acid, theobromine, GC, protocatechuic acid, theophylline, EGC, theanine, chlorogenic acid, caffeine, catechin, syringic acid, caffeic acid, vanillic acid, epicatechin, EGCG, delphinidin, salicylic acid, GCG, p-coumaric acid, cyanidin, ferulic acid, sinapic acid, rutin, ECG, pelargonidin, myricetin, theaflavin, quercetin, apigenin and kaempferol. The application can realize simultaneous rapid qualitative and quantitative detection of the 30 compounds, and provides technical support for development of active substances in tea.

According to the invention, a ternary eutectic solvent is combined with a microwave extraction method to extract anthocyanin components in red beans and cowberries. And the cyanidin-3-O-glucoside and the delphinidin-3-O-glucoside, which are relatively high in content, in the vaccinium ormosia are quantified by using an ultra-high performance liquid chromatograph. The contents of the cyanidin-3-O-glucoside and the delphinidin-3-O-glucoside extracted by the method disclosed by the invention are relatively high and are very stable. The selection of the type, molar ratio, water content, solid-liquid ratio, extraction temperature and extraction time of the eutectic solvent is optimized by a single factor test, and the content of choline chloride-citric acid-1, 4Bu in the molar ratio of 1: 2: 2 is obtained by an orthogonal test when the water content is 15%, the solid-liquid ratio is 1: 30, the extraction temperature is 50 DEG C and the extraction time is 25 minutes, and the content of the choline chloride-citric acid-1, 4Bu in the choline chloride-citric acid-1, 4Bu in the choline chloride-citric acid-1, 4Bu in the choline chloride-citric acid-1, 4Bu is 5%. The highest content of the extracted cyanidin-3-O-glucoside ranges from 1.97 mg / g to 2.8 mg / g, and the highest content of the extracted delphinidin-3-O-glucoside ranges from 5.93 mg / g to 6.8 mg / g. And the extracted red bean and bilberry anthocyanin extracting solution has relatively high antioxidant activity and can be applied to the field of oxidation resistance.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus reuteri XS82 and application thereof in preparation of gastrodia elata leavening with anti-inflammation and high in-vivo and in-vitro bioavailability. The gastrodia elata fermented product prepared by fermenting the lactobacillus reuteri mucus XS82 has high-content gastrodin and parishin E and has antioxidant and anti-inflammatory effects, in-vitro digestion experiments verify that the bioavailability of p-hydroxybenzyl alcohol is remarkably improved, and rat experiments verify that the bioavailability of gastrodin can be remarkably improved, so that the gastrodia elata fermented product can be used for preparing the medicine for treating gastrodia elata. Moreover, the content of effective components such as cockscomb lactone C, 18-deoxycyanidin, caffeoyl feruloyl spermidine, dogwood glycoside and the like in serum can be increased, and the improvement of anti-inflammatory and antioxidant functions is promoted.

The application discloses application of a SlAN2-like gene in regulating content of delphinidin-3-glucoside in tomatoes, a nucleotide sequence of the SlAN2-like gene is shown as SEQ ID NO:1, and overexpression of the SlAN2-like gene can increase the content of delphinidin-3-glucoside in the tomatoes. Compared with the prior art, the application finds that overexpression of the SlAN2-like gene can increase the content of delphinidin-3-glucoside in the tomatoes, and only one anthocyanin of delphinidin-3-glucoside is produced. Therefore, the SlAN2-like gene can be used to produce a tomato material containing only delphinidin-3-glucoside monomers, because anthocyanins in most plants are in the form of a mixture, and extraction and purification work is needed to obtain monomer anthocyanin substances. The created tomato material can be used to reduce technical barriers and costs of extracting delphinidin-3-glucoside.

The present application belongs to the technical field of plant extracts, and particularly relates to an antioxidant plant extract composition, which is prepared by mixing the following raw materials in parts by mass: Passiflora edulis fruit extract 30-40 parts, blackcurrant fruit extract 25-35 parts, and Ampelopsis megalophylla leaf extract 20-30 parts. The present application first compounds three plant extracts of Passiflora edulis fruit, blackcurrant fruit and Ampelopsis megalophylla leaf, which can enrich the types of related products. Moreover, Passiflora edulis fruit is rich in flavones such as apigenin and luteolin, blackcurrant fruit is rich in anthocyanins such as cyanidin and delphinidin, and Ampelopsis megalophylla leaf is rich in flavonols such as dihydromyricetin. The synergistic effect of the three extracts can scavenge mitochondrial ROS, protect mitochondrial membrane potential, and improve the activity of respiratory chain complex enzymes, and has good mitochondrial protection and antioxidant damage repair effects, and has important application value for the protection of mitochondrial oxidative damage in the middle-aged and elderly population.