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Transformed plant having blue flower color, and method for creating same

A plant and gene technology, applied in the field of transformed plants with blue flower colors and its production, can solve the problems of inability to make roses and faded flower colors, etc.

Pending Publication Date: 2020-07-07
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when the flavone synthase gene derived from gentian was expressed in tobacco, flavones were synthesized (Non-Patent Document 8), but the flower color became pale
Furthermore, attempts to change the flower color of roses by artificially containing flavonoids and delphinidin are in progress (Patent Document 2), but roses with blue flower colors have not yet been successfully produced.
[0008] Actually, in the flower color change of roses aiming at the blue flower color so far, purple (hue group under the RHS color spectrum: Purple group) or blue-violet (Purple-Violet group, Violet group) has a limit and cannot be produced Blue roses with purple-blue (Violet-Blue group) or blue (Blue group) flower color

Method used

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  • Transformed plant having blue flower color, and method for creating same
  • Transformed plant having blue flower color, and method for creating same
  • Transformed plant having blue flower color, and method for creating same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0292] [Example 1: Simulation of Copigment Effect of Flavonoid C-Glycosides on Anthocyanins]

[0293] In order to simulate the copigment effect of flavone C-glycoside on anthocyanin, anthocyanin and flavone C-glycoside were adjusted. Delphinidin (delphinidin 3,5-diglycoside) and isovitexin (apigenin 6-C-glycoside) used in this experiment were purchased from Funakoshi Corporation.

[0294] With respect to the anthocyanins thus obtained (dimethinin), flavonoid C-glucoside (isovititexin) was added at a molar concentration ratio of 0, 2, and 4 equivalents in a buffer solution of pH 4.5, and the absorption Spectra were measured. The concentration of anthocyanins was set at 0.5 mM.

[0295] The absorbance of anthocyanin aqueous solution was increased by adding flavone C-glucoside, and the absorption extremum (λmax) moved to the long wavelength side with the addition of flavone C-glucoside. From this, it is clear that delphinidin is affected by the copigment effect of isovitexin. ...

Embodiment 2

[0299] [Example 2: (Route 1) Introducing pansy-derived F3'5'H#40 gene, licorice-derived F2H gene, rice-derived CGT gene, and licorice-derived FDH gene into rose variety "Rita Perfumera"]

[0300] pSPB4743 uses pBINPLUS as the basic backbone and contains the following four expression cassettes.

[0301] (1) El 2 35S promoter and F3'5'H full-length cDNA (SEQ ID NO: 1) and D8 terminator from Pansy

[0302] (2) 35S promoter and F2H full-length cDNA (SEQ ID NO: 3) from licorice and AT terminator from perilla

[0303] (3) 35S promoter and CGT full-length cDNA (SEQ ID NO: 5) from rice and AT terminator from perilla

[0304] (4) 35S promoter and FDH full-length cDNA (SEQ ID NO: 7) from licorice and AT terminator from perilla

[0305] The plasmid can constitutively express the F3'5'H#40 gene of pansy, the F2H gene of licorice, the CGT gene of rice and the FDH gene of licorice in plants.

[0306] The thus produced pSPB4743 was introduced into the orange rose variety "Rita·Perfumera"...

Embodiment 3

[0315] [Example 3: (Route 1) Introducing the F3'5'H#40 gene from pansy and the F2H gene from licorice, the CGT gene from rice, and the FDH gene from licorice into the rose variety "noble"]

[0316] The pSPB4743 produced in the same manner as in Example 2 was introduced into the pink rose variety "Noble" to obtain a total of 20 transformants. Pigment analysis was carried out on these, and as a result, accumulation of delphinidin was confirmed in all the individuals, and the delphinidin content rate was the highest at 88% (83.5% on average). Furthermore, in 18 of these individuals, it was confirmed that isovitexin, which is a flavonoid C-glycoside, was produced in the highest amount of 0.06 mg per 1 g of the fresh weight of petals.

[0317] The analytical values ​​of representative transformants are shown in Table 3 below.

[0318] [table 3]

[0319]

[0320] Host: Noble

[0321] Del: delphinidin, Cya: cyanidin, Pel: geranium

[0322] M: Myricetin, Q: Quercetin, K: Kaempf...

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Abstract

Provided is: a transformed plant that has blue flower color; a self-fertilized or cross-fertilized progeny of the transformed plant; or a propagule, a portion of a plant body, tissue, or cells of thetransformed plant or the self-fertilized or cross-fertilized progeny of the transformed plant. The petals of the present invention co-express delphinidin anthocyanin and a flavone C-glycoside.

Description

technical field [0001] The present invention relates to a method, which is a method for producing a transformed plant having a blue flower color, characterized in that delphinidin-type anthocyanin (anthocyanin) and flavone C-glucoside coexist in the cells of the plant, and relates to A transformed plant characterized by the coexistence of delphinidin-type anthocyanins and flavone C-glycosides in cells, or its self-propagating or other progeny, or their vegetative propagules, a part of the plant (especially cut flowers) or its Processed products (especially processed products of cut flowers), tissues or cells. Background technique [0002] Roses, chrysanthemums, carnations, etc. are important flowers in the world industry. In particular, rose is the most popular flower plant, and it has been recorded to have been cultivated since BC, and it has been artificially improved over hundreds of years. However, it has been difficult to produce rose varieties with blue flower colors...

Claims

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Application Information

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IPC IPC(8): C12N15/09A01H5/00C12N15/52C12N15/63A01H6/74
CPCC12N15/09C12N15/52C12N15/63C12N9/88C12Y114/13088C12Y114/11022C12N9/1007C12Y101/01001C12N15/825Y02A40/146
Inventor 中村典子兴津奈央子田中良和
Owner SUNTORY HLDG LTD
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