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VlbZIP30 gene for promoting synthesis of grape anthocyanin and application of VlbZIP30 gene

A grape flower and green glycoside technology is applied in the field of genetic engineering to achieve the effect of improving biological functions

Active Publication Date: 2022-04-19
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the bZIP transcription factor family genes have many important biological functions, such as the expression of seed storage genes, plant growth and development, light signal transduction, disease defense, abiotic stress response, etc., but there is still little research on the function of grape bZIP genes. few

Method used

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  • VlbZIP30 gene for promoting synthesis of grape anthocyanin and application of VlbZIP30 gene
  • VlbZIP30 gene for promoting synthesis of grape anthocyanin and application of VlbZIP30 gene
  • VlbZIP30 gene for promoting synthesis of grape anthocyanin and application of VlbZIP30 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Overexpression of VlbZIP30 significantly increases the content of anthocyanins in seedless white grapes

[0037] Through phenotype observation, it was found that compared with the wild control, the leaves and stems of the transgenic plants were significantly reddened. After content extraction, it was found that the anthocyanin content in the leaves and stems of the transgenic plants was significantly higher than that of the wild control ( figure 1 ).

[0038] In order to further understand which anthocyanin metabolites VlbZIP30 is specifically involved in the accumulation of, wide-target metabolome assays were performed on 4-month-old wild-type controls and transgenic plants, and it was found that under normal growth conditions, compared with wild controls, A total of 435 metabolites were different in the transgenic plants, of which 227 metabolites were significantly increased and 208 metabolites were decreased. The inventor made statistics on the significa...

Embodiment 2

[0039] Example 2: VlbZIP30 improves the synthesis of grape anthocyanins by regulating the expression of key genes for anthocyanin biosynthesis

[0040] In order to further understand the mechanism of action of VlbZIP30 involved in anthocyanin biosynthesis, this study performed transcriptome sequencing on 4-month-old wild-type and transgenic plants, and found that under normal growth conditions, compared with wild-type There were 1796 differentially expressed genes up-regulated and 1398 differentially expressed genes down-regulated. Through the correlation analysis of transcriptome and metabolome data, it was found that the expression of genes in the anthocyanin biosynthesis pathway (1 CHS, VIT_05s0136g00260; 1 CHI, VIT_13s0067g03820; 1 F3'5'H, VIT_08s0007g05160; 1 F3′HVIT_17s0000g07200; One F3H, VIT_04s0023g03370; one DFR gene VIT_16s0039g02350) was generally higher in #25 than WT, and the corresponding metabolites, such as chalcone, naringenin, eriodictyol, dihydroquercetin, ...

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Abstract

The invention discloses a VlbZIP30 gene for promoting synthesis of grape anthocyanin and application of the VlbZIP30 gene. The full length of a complete open reading frame sequence of the gene is 978bp, and 325 amino acids are encoded. The method comprises the following steps: constructing a pCAMBIA2300-35s-3 * Flag-VlbZIP30 overexpression vector, and stably transferring the pCAMBIA2300-35s-3 * Flag-VlbZIP30 overexpression vector into Thomson'seedless white 'grapes through an agrobacterium-mediated grape genetic transformation method. The result shows that the content of anthocyanin in leaves and stems of an overexpressed transgenic plant is remarkably increased, and the gene is mainly used for promoting the expression of cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, paeonin-3-O-glucoside and morning glorybin-3-O-glucoside by regulating and controlling the expression of a key gene for biosynthesis of the anthocyanin, so that the yield of the anthocyanin is increased, and the yield of the anthocyanin is increased. The synthesis of anthocyanin such as malvidin-3-O-glucoside improves the accumulation of anthocyanin in plants, thereby promoting the leaves and stems of the white grape variety to turn red from green.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a VlbZIP30 gene for promoting the synthesis of grape anthocyanins and an application thereof. Background technique [0002] Anthocyanins are a class of water-soluble flavonoids that are found in almost all vascular plants and have been shown to have many important biological functions. For example, they appear orange, red, purple, and blue depending on the pH value, and are widely present in the fruits, seeds, petals, roots, and leaves of plants, so they are beneficial in attracting pollinators (insects or animals) Plants play an important role in the dispersal of pollen and seeds; anthocyanins also act as protective agents against UV rays and participate in plant stress responses. In addition, anthocyanins also have a strong antioxidant capacity, providing humans with a variety of health-promoting capabilities, such as anti-aging, treating cardiovascular...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/88
CPCC07K14/415C12N15/825
Inventor 涂明星王西平赵睿康王现行
Owner NORTHWEST A & F UNIV
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