16 results about "Anthocyanin biosynthesis" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a related biological product of an LhHB4 gene in regulation and control of accumulation of anthocyanin in plants. The nucleotide sequence of the LhHB4 gene is as shown in SEQ ID NO. 1, and the sequence of the encoded protein is as shown in SEQ ID NO. 2. Experiments show that the expression of the LhHB4 is in negative correlation with the biosynthesis of the anthocyanin of the lily, the accumulation of the anthocyanin in the lilies in the lilies can be remarkably inhibited by the instantaneous overexpression of the LhHB4 in the lilies, the accumulation of the anthocyanin in the lilies in the lilies in the leaves is promoted after the LhHB4 gene is silenced, and the regulation of the LhHB4 on the biosynthesis of the anthocyanin depends on an EAR motif. The related biological product of the LhHB4 gene provided by the invention provides important application support for cultivating new varieties with peculiar colors of lilies and improving the ornamental and commercial values of the lilies.

This invention relates to the field of plant genetic engineering technology, and discloses the application of the DkHOS15 gene in regulating the biosynthesis of persimmon proanthocyanidins. The nucleotide sequence of the DkHOS15 gene is shown in SEQ ID NO.1, or is a sequence in which one or more nucleotides are deleted, added, and / or substituted, yet still retain the function of positively regulating the biosynthesis of persimmon proanthocyanidins. This invention confirms that DkHOS15 is a positively regulating transcription factor gene for the synthesis of persimmon proanthocyanidins, and has the function of promoting the accumulation of persimmon proanthocyanidins. It provides a key gene resource for the targeted regulation of persimmon proanthocyanidins, and is of great significance for optimizing the tannin content of persimmon fruit, improving the quality of persimmon fruit, and promoting the improvement of persimmon germplasm.

The application belongs to the field of biotechnology, and particularly relates to application of a wax plant CpMADS1 gene in regulating biosynthesis of plant anthocyanins. The wax plant CpMADS1 gene belongs to the MADS-box family, and its expression is closely related to flower color formation of the wax plant, but its function in heterologous plants such as tobacco has not been revealed. In the application, the wax plant CpMADS1 gene is introduced into tobacco, and it is found that the gene significantly inhibits synthesis of anthocyanins, and is accompanied by slight down-regulation of flavonoid content. Research shows that CpMADS1 may affect downstream metabolic flux distribution by regulating expression of key enzyme genes in the anthocyanin synthesis pathway, so as to change the flower color phenotype. The application provides a new strategy for regulating plant anthocyanin synthesis by using MADS-box genes, and has important application value for flower breeding and pigment metabolism engineering.

The application provides application of MdPHY7 protein or MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of apple anthocyanin synthesis, and belongs to the technical field of plant genetic engineering. Exogenous ALA treatment can induce MdPHY7 gene expression, thereby promoting anthocyanin synthesis increase, finally causing apple anthocyanin accumulation and promoting fruit coloring. It has been verified that overexpression of MdPHY7 gene can promote apple anthocyanin accumulation, while interference with MdPHY7 gene expression can inhibit anthocyanin accumulation and further inhibit fruit appearance quality improvement. It can be seen that MdPHY7 is a new key gene for regulating apple anthocyanin accumulation, and plays a key role in ALA regulation of apple anthocyanin accumulation, which has far-reaching significance for agricultural and forestry production, and lays a solid theoretical foundation for application of ALA in the field of agricultural production.

The invention discloses an R2R3-MYB transcription factor for specifically regulating and controlling anthocyanin accumulation in a spotted region of a dendrobium nobile flower and application of the R2R3-MYB transcription factor, the R2R3-MYB transcription factor comprises an R2R3-MYB gene-DnMYB1, and the application of the R2R3-MYB transcription factor comprises the following steps: carrying out targeted regulation and control through a transgenic technology, and carrying out transgenic breeding; in the phalaenopsis and white flower gardening variety, the heterologous transient overexpression of the target gene can significantly promote the accumulation of anthocyanin in petals of the phalaenopsis and white flower gardening variety; in the wild type dendrobium nobile, the accumulation of the anthocyanin in the floral quilt of the wild type dendrobium nobile can be obviously reduced through targeted silencing of the target gene. The gene plays an important role in the aspect of anthocyanin biosynthesis, can enrich a transgenic material library in which specific pigments are accumulated in a spot region of the orchid, and provides powerful support for molecular breeding for cultivating a diversity coloring mode of the orchid in the future.

The present application belongs to the field of biotechnology, and particularly relates to Lycium ruthenicum Murr LrCHS1 gene and application of the coded protein in promoting anthocyanin biosynthesis. The present application firstly finds that LrCHS1 gene can promote accumulation of anthocyanin compounds, and can be used for positive mediation of Lycium ruthenicum anthocyanin biosynthesis. Anthocyanin can be obtained through the biosynthesis pathway by overexpressing LrCHS1 gene in plants, and a new method for obtaining anthocyanin is provided. The construction method of the recombinant plasmid and the recombinant bacteria of overexpressing Lycium ruthenicum Murr LrCHS1 gene can obtain the recombinant expression vector of overexpressing LrCHS1 gene, and the obtained recombinant expression vector can improve the content of anthocyanin in plants, and can be used for biosynthesis of anthocyanin.

Provided is a polypeptide for promoting plant anthocyanin synthesis, comprising a polypeptide obtained by mutating a key site in a key domain for regulating anthocyanin synthesis. By performing key-site mutation and optimization on an existing sequence, and using the resulting polypeptide to promote anthocyanin biosynthesis, it has been verified that the polypeptide can significantly improve the regulatory ability of a transcription factor in the anthocyanin biosynthesis. An overexpression vector is also constructed on the basis of a coding gene or optimized gene for the polypeptide. By overexpressing the gene or optimized gene in a plant cell, anthocyanin accumulation in a transgenic material can be significantly promoted.

The application discloses application of NcolMYB75-like gene of blue star water lily in improving anthocyanin content of plants and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the NcolMYB75-like gene provided in the application is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. The application finds that the gene can strongly promote anthocyanin biosynthesis by heterologous expression of the gene in Arabidopsis thaliana AtMYB75 function loss mutant (myb75-c), so that the anthocyanin accumulation amount of the transgenic plant reaches more than 15 times of that of a wild type control, and the leaves present a significant purplish red color. The application is particularly suitable for plant materials with limited anthocyanin synthesis capacity, and provides an innovative gene resource and technical scheme for efficient cultivation of crop and ornamental plant varieties with high anthocyanin content.

The invention discloses a pear transcription factor PyHAT5 and application of the pear transcription factor PyHAT5 in synthesis of red-flesh pear anthocyanin. The transcription factor PyHAT5 capable of regulating and controlling pulp anthocyanin biosynthesis in the red-pulp pears belongs to an HD-ZIP family member, the nucleotide sequence of the transcription factor PyHAT5 is as shown in SEQ ID No.1, and the amino acid sequence coded by the transcription factor PyHAT5 is as shown in SEQ ID No.2 in a sequence table. Through instantaneous overexpression in pear fruits, the PyHAT5 is proved to be capable of inhibiting accumulation of anthocyanin in the pear fruits; and the accumulation of the anthocyanin in the pear fruits can be promoted by instantaneously silencing the PyHAT5. The discovery of the gene supplements and perfects an anthocyanin synthesis regulation mechanism of HD-ZIP transcriptional regulation in pears, and the effect of the PyHAT5 gene on inhibiting accumulation of the flesh anthocyanin can be relieved through technologies such as gene editing and RNAi in the future, so that synthesis of the flesh anthocyanin of the pears is promoted, and a gene resource and a theoretical basis are provided for breeding of the red-flesh pears.

The application discloses a RsLNC2361 gene for regulating synthesis of radish anthocyanin and application thereof, and belongs to the technical field of genetic engineering.The application clones the RsLNC2361 gene from a purple skin and red flesh radish.The transient overexpression of the gene in radish leaves can increase the anthocyanin accumulation content in the radish leaves and significantly improve the expression level of anthocyanin biosynthesis related genes, and silencing of the RsLNC2361 in radish root can reduce the anthocyanin accumulation in the flesh root and the expression level of anthocyanin biosynthesis genes.The application not only helps to accelerate the radish breeding process and directionally cultivate a new radish variety with high anthocyanin content, but also provides an important theoretical basis for analyzing the molecular regulation mechanism of radish anthocyanin synthesis.

The invention discloses application of different transcripts of potato StB3-like in anthocyanin biosynthesis, and belongs to the technical field of biology. According to the invention, SMRT sequencing and RNA-seq technology are integrated, systematic research of three key development stages is carried out on three kinds of potatoes with different colors, and by analyzing a variable splicing mode of transcription factors, 33 variable splicing events are found, and StB3-like is a key gene of the invention. Experiments show that after co-expression with StAN1, StB3-like-2 and StB3-like-3 significantly increase the anthocyanin accumulation amount, while StB3-like-1 does not have the effect. Therefore, different transcripts of the same gene are proved, and anthocyanin accumulation is regulated and controlled through StAN1 dependency mechanism difference. The method disclosed by the invention has a very wide application prospect in the aspects of crop anthocyanin biosynthesis breeding and subsequent preparation of anthocyanin-containing health-care products.

Disclosed is use of an MdPHY7 protein or an MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of the apple anthocyanin biosynthesis, belonging to the technical field of plant genetic engineering. Exogenous 5-aminolevulinic acid (ALA) treatment can induce expression of the MdPHY7 gene, thereby promoting an increase of the apple anthocyanin biosynthesis, and then ultimately resulting in accumulation of apple anthocyanins to promote apple fruit coloring.

The invention discloses a dehydration response element binding protein FtDREB02 as well as a coding gene and application thereof. The dehydration response element binding protein FtDREB02 and the coding gene thereof are separated from tartary buckwheat through molecular cloning, the amino acid sequence of the dehydration response element binding protein FtDREB02 is shown as SEQ ID No.2, and the nucleotide sequence of the coding gene of the dehydration response element binding protein FtDREB02 is shown as SEQ ID No.1. Genetic transformation experiments prove that the encoding gene of the dehydration response element binding protein FtDREB02 can remarkably improve the plant drought tolerance, has the dual functions of enhancing the promoter activity of the anthocyanin synthase gene, and has application prospects in the aspects of improving the plant drought stress resistance or regulating the plant anthocyanin biosynthesis and the like.

The invention provides a polypeptide for promoting synthesis of plant anthocyanin and application, and belongs to the technical field of genetic engineering. The invention provides a polypeptide for promoting the synthesis of plant anthocyanin. The polypeptide is characterized in that a key site of a key structural domain for regulating and controlling the synthesis of the anthocyanin is mutated. According to the invention, the existing sequence is subjected to key site mutation and optimization, and is used for promoting the biosynthesis of anthocyanin. By mutating the key amino acid site of the original sequence, the regulation and control capability of the transcription factor in anthocyanin biosynthesis can be obviously improved. The invention also constructs an overexpression vector based on the coding gene or the optimized gene of the polypeptide, and the anthocyanin accumulation amount of a transgenic material can be obviously promoted by overexpressing the gene or the optimized gene in a plant cell.

The invention relates to the technical field of biology, in particular to a wheat TaTT8 gene related to wheat anthocyanin biosynthesis and application of a promoter of the wheat TaTT8 gene. The invention provides application of a protein TaTT8 or a biological material related to the protein TaTT8, and the application is any one of Y1-Y3: Y1, application in regulating and controlling the color of wheat grains; y2 in increasing the anthocyanin content of wheat; y3 is applied to cultivation of purple wheat or cultivation of wheat with high anthocyanin content. A TaTT8 overexpression wheat material is obtained to prove that TaTT8 is a key factor of color change of purple wheat seed coats, but a TaTT8 gene coding sequence in purple wheat is not different from that in white wheat, and a specific cis-acting element exists in a TaTT8 gene promoter region in the purple wheat to improve expression of TaTT8, so that biosynthesis of wheat anthocyanin is promoted, and the yield of wheat anthocyanin is increased. Purple wheat germplasm resources are innovated, and the method is of great significance to improvement of grain quality.