35 results about "Anthocyanin biosynthesis" patented technology

The invention discloses a poplar PdbHLH57 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the poplar PdbHLH57 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein of the poplar PdbHLH57 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an expression vector of a poplar PdbHLH57 gene, and transforming the expression vector into a poplar leaf; and cultivating and screening to obtain the transgenic poplar with red leaves. Results show that compared with wild poplar, the PdbHLH57 gene for promoting poplar anthocyanin synthesis has the advantages that transgenic poplar leaves become red, and the anthocyanin content in transgenic plant leaves is remarkably increased; the expression level of anthocyanin biosynthesis related genes is remarkably increased, and further, it is shown that the PdbHLH57 gene plays an important role in poplar anthocyanin biosynthesis.

The invention belongs to the field of plant gene engineering, and particularly relates to a method for constructing an anthocyanin plant cell factory by using VdUFGT overexpression, a cloned vitis davidii VdUFGT gene is subjected to autogenetic transformation to obtain a VdUFGT high-expression and anthocyanin high-yield cell line, and the cell line remarkably improves the expression of anthocyanin biosynthesis key genes, so that the yield of anthocyanin is improved. And in combination with cell suspension culture, a high-yield anthocyanin plant cell factory is constructed. According to the method, the anthocyanin yield can be increased by 8.5 times and reaches 3.17%, the anthocyanin production period can be shortened to 12 days, the method has the advantages of being low in cost, short in period, high in efficiency, environmentally friendly and the like, and large-scale annual uninterrupted production of natural anthocyanin can be achieved.

The invention discloses an application of transcription factors AcMYB266 and AcbHLH901 in regulation and control of synthesis of anthocyanin in pineapple peel. The nucleotide sequence of the transcription factor AcMYB266 is shown as a sequence table SEQ ID NO: 1, and the amino acid sequence of the transcription factor AcMYB266 is shown as a sequence table SEQ ID NO: 2. The transcription factor AcMYB266 is a key transcription regulation factor for biosynthesis of anthocyanin in bracts and calyx of the pineapple, and the AcbHLH901 can interact with the AcMYB266, so that the expression activity of AcANS synthesized in the later stage in a pineapple anthocyanin biosynthesis pathway is improved, anthocyanin synthesis is precisely regulated and promoted, and anthocyanin accumulation of the bracts and sepals of the pineapple is positively regulated. The invention discloses a molecular regulation mechanism of specific accumulation of anthocyanin in bracts and calyxes of pineapples, and provides a new idea for creating a new variety of high-quality red-skin edible pineapples.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a related biological product of an LhHB4 gene in regulation and control of accumulation of anthocyanin in plants. The nucleotide sequence of the LhHB4 gene is as shown in SEQ ID NO. 1, and the sequence of the encoded protein is as shown in SEQ ID NO. 2. Experiments show that the expression of the LhHB4 is in negative correlation with the biosynthesis of the anthocyanin of the lily, the accumulation of the anthocyanin in the lilies in the lilies can be remarkably inhibited by the instantaneous overexpression of the LhHB4 in the lilies, the accumulation of the anthocyanin in the lilies in the lilies in the leaves is promoted after the LhHB4 gene is silenced, and the regulation of the LhHB4 on the biosynthesis of the anthocyanin depends on an EAR motif. The related biological product of the LhHB4 gene provided by the invention provides important application support for cultivating new varieties with peculiar colors of lilies and improving the ornamental and commercial values of the lilies.

The present invention belongs to the technical field of natural material synthesis, and specifically relates to a method for increasing the content of proanthocyanidins in Spirodela. In order to promote the application of Spirodela in ecological engineering, the present invention discloses a method for increasing the content of proanthocyanidins in Spirodela, wherein the proanthocyanidin content of Spirodela is increased by culturing Spirodela with a sugar-containing culture medium, culturing Spirodela with a hormone-containing culture medium, culturing Spirodela with light of different wavelengths, or culturing Spirodela with a nitrogen-deficient and / or phosphorus-deficient culture medium, and the proanthocyanidin content of Spirodela can also be increased by increasing the expression of genes such as 4CL1, F3'H1, F3'H3, F3'H4, ANS1, PAL1, F3'H2, ANR3 and F3H through genetic engineering means. The method of the present invention is helpful for developing and utilizing Spirodela to produce proanthocyanidins, turning Spirodela into treasure, and also provides a theoretical basis for the molecular mechanism of environmental factors regulating the biosynthesis of proanthocyanidins in Spirodela.

This invention relates to the field of plant genetic engineering technology, and discloses the application of the DkHOS15 gene in regulating the biosynthesis of persimmon proanthocyanidins. The nucleotide sequence of the DkHOS15 gene is shown in SEQ ID NO.1, or is a sequence in which one or more nucleotides are deleted, added, and / or substituted, yet still retain the function of positively regulating the biosynthesis of persimmon proanthocyanidins. This invention confirms that DkHOS15 is a positively regulating transcription factor gene for the synthesis of persimmon proanthocyanidins, and has the function of promoting the accumulation of persimmon proanthocyanidins. It provides a key gene resource for the targeted regulation of persimmon proanthocyanidins, and is of great significance for optimizing the tannin content of persimmon fruit, improving the quality of persimmon fruit, and promoting the improvement of persimmon germplasm.

The application belongs to the field of biotechnology, and particularly relates to application of a wax plant CpMADS1 gene in regulating biosynthesis of plant anthocyanins. The wax plant CpMADS1 gene belongs to the MADS-box family, and its expression is closely related to flower color formation of the wax plant, but its function in heterologous plants such as tobacco has not been revealed. In the application, the wax plant CpMADS1 gene is introduced into tobacco, and it is found that the gene significantly inhibits synthesis of anthocyanins, and is accompanied by slight down-regulation of flavonoid content. Research shows that CpMADS1 may affect downstream metabolic flux distribution by regulating expression of key enzyme genes in the anthocyanin synthesis pathway, so as to change the flower color phenotype. The application provides a new strategy for regulating plant anthocyanin synthesis by using MADS-box genes, and has important application value for flower breeding and pigment metabolism engineering.

The application provides application of MdPHY7 protein or MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of apple anthocyanin synthesis, and belongs to the technical field of plant genetic engineering. Exogenous ALA treatment can induce MdPHY7 gene expression, thereby promoting anthocyanin synthesis increase, finally causing apple anthocyanin accumulation and promoting fruit coloring. It has been verified that overexpression of MdPHY7 gene can promote apple anthocyanin accumulation, while interference with MdPHY7 gene expression can inhibit anthocyanin accumulation and further inhibit fruit appearance quality improvement. It can be seen that MdPHY7 is a new key gene for regulating apple anthocyanin accumulation, and plays a key role in ALA regulation of apple anthocyanin accumulation, which has far-reaching significance for agricultural and forestry production, and lays a solid theoretical foundation for application of ALA in the field of agricultural production.

The invention belongs to the technical field of biology, and particularly relates to application of a lycium ruthenicum LrAOMT gene and an encoding protein thereof in regulation and control of lycium ruthenicum anthocyanin biosynthesis. Research finds that the LrAOMT gene can participate in biosynthesis of anthocyanin and regulate accumulation of anthocyanin compounds for the first time. Through overexpression of the LrAOMT gene, biosynthesis of the anthocyanin compounds can be selectively regulated and controlled, and a new technical means is provided for obtaining the anthocyanin compounds. According to the construction method of the recombinant plasmid and the recombinant bacterium for overexpressing the lycium ruthenicum LrAOMT gene, a recombinant expression vector for overexpressing the LrAOMT gene can be obtained, and the obtained recombinant expression vector can be applied to biosynthesis of anthocyanin compounds.

The invention discloses an R2R3-MYB transcription factor for specifically regulating and controlling anthocyanin accumulation in a spotted region of a dendrobium nobile flower and application of the R2R3-MYB transcription factor, the R2R3-MYB transcription factor comprises an R2R3-MYB gene-DnMYB1, and the application of the R2R3-MYB transcription factor comprises the following steps: carrying out targeted regulation and control through a transgenic technology, and carrying out transgenic breeding; in the phalaenopsis and white flower gardening variety, the heterologous transient overexpression of the target gene can significantly promote the accumulation of anthocyanin in petals of the phalaenopsis and white flower gardening variety; in the wild type dendrobium nobile, the accumulation of the anthocyanin in the floral quilt of the wild type dendrobium nobile can be obviously reduced through targeted silencing of the target gene. The gene plays an important role in the aspect of anthocyanin biosynthesis, can enrich a transgenic material library in which specific pigments are accumulated in a spot region of the orchid, and provides powerful support for molecular breeding for cultivating a diversity coloring mode of the orchid in the future.

A pear transcription factor that inhibits anthocyanin biosynthesis PyERF4.1 gene, its recombinant expression vector and application, wherein the PyERF4.1 nucleotide sequence of the gene is shown in SEQ ID No.1. In the present invention, the transcription factor PyERF4.1 was overexpressed and knocked out in pear callus and tomato fruits to verify its function of inhibiting anthocyanin biosynthesis, and PyERF4.1 was PyERF3, PyMYB114, and PybHLH3 co-transformed with the PyERF4.1 gene into pear fruits and strawberry fruits, resulting in reduced anthocyanin accumulation in pear fruits and strawberry fruits. Through biological function verification, it is shown that the PyERF3, PyMYB114, and PybHLH3 gene cloned in the present invention interacts with the PyERF4.1 gene to inhibit the function of anthocyanin biosynthesis in pear peel.

A pear transcription factor that inhibits anthocyanin biosynthesis PyERF4.2 Gene and its recombinant expression vector and application, the PyERF4.2 The nucleotide sequence of the gene is shown in SEQ ID No. 1. PyERF4.2 pear callus to verify its function of inhibiting anthocyanin biosynthesis and PyERF4.2 and PyERF3, PyMYB114, and PybHLH3 Gene co-transformation of pear and strawberry fruits resulted in reduced anthocyanin accumulation in pear and strawberry fruits, and biological function verification showed that the cloned PyERF4.2 and PyERF3, PyMYB114, and PybHLH3 Genetic interactions inhibit the function of anthocyanin biosynthesis in pear peel.

The invention relates to the technical field of agricultural biology, and discloses a method for increasing the anthocyanin content of purple sweet potato tuberous roots. According to the method, the endogenous IbMYB27 gene of the purple sweet potato is subjected to interference or knockout or expression weakening, so that the genetic engineering purple sweet potato with the root tuber anthocyanin content increased is obtained. Researches show that the IbMYB27 gene interferes and expresses that the content of anthocyanin in sweet potato peel is 179.2 mg / 100g, the content of anthocyanin in sweet potato pulp is 35.41 mg / 100g, and the content of anthocyanin in sweet potato peel and the content of anthocyanin in sweet potato pulp are obviously higher than those of an Aya wild type. The results show that the IbMYB27 can inhibit the biosynthesis of the anthocyanin induced by the IbMYB1.

The present application belongs to the field of biotechnology, and particularly relates to Lycium ruthenicum Murr LrCHS1 gene and application of the coded protein in promoting anthocyanin biosynthesis. The present application firstly finds that LrCHS1 gene can promote accumulation of anthocyanin compounds, and can be used for positive mediation of Lycium ruthenicum anthocyanin biosynthesis. Anthocyanin can be obtained through the biosynthesis pathway by overexpressing LrCHS1 gene in plants, and a new method for obtaining anthocyanin is provided. The construction method of the recombinant plasmid and the recombinant bacteria of overexpressing Lycium ruthenicum Murr LrCHS1 gene can obtain the recombinant expression vector of overexpressing LrCHS1 gene, and the obtained recombinant expression vector can improve the content of anthocyanin in plants, and can be used for biosynthesis of anthocyanin.

Provided is a polypeptide for promoting plant anthocyanin synthesis, comprising a polypeptide obtained by mutating a key site in a key domain for regulating anthocyanin synthesis. By performing key-site mutation and optimization on an existing sequence, and using the resulting polypeptide to promote anthocyanin biosynthesis, it has been verified that the polypeptide can significantly improve the regulatory ability of a transcription factor in the anthocyanin biosynthesis. An overexpression vector is also constructed on the basis of a coding gene or optimized gene for the polypeptide. By overexpressing the gene or optimized gene in a plant cell, anthocyanin accumulation in a transgenic material can be significantly promoted.

The invention provides application of potato miR828 in regulation and control of anthocyanin biosynthesis, and relates to the technical field of biology, a nucleotide sequence of a precursor sequence of the potato miR828 is as shown in SEQ ID NO.1, and a mature sequence of the potato miR828 is as shown in SEQ ID NO.2. According to the present invention, the cutting effect of the miR828 on the StMYB3 is proved through the dual luciferase and the in vivo degradation experiment; tobacco transient expression analysis experiments find that miR828 may be a negative regulatory factor for anthocyanin biosynthesis; a dual luciferase experiment shows that after the miR828 is co-injected, the activity of the StDFR promoter is reduced; a luciferase complementation experiment shows that the miR828 inhibits the formation of a compound, and the miR828 has great significance in enriching anthocyanin biosynthesis regulation ideas, crop anthocyanin biosynthesis breeding and subsequent preparation of anthocyanin-containing health-care products.

The application discloses application of NcolMYB75-like gene of blue star water lily in improving anthocyanin content of plants and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the NcolMYB75-like gene provided in the application is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. The application finds that the gene can strongly promote anthocyanin biosynthesis by heterologous expression of the gene in Arabidopsis thaliana AtMYB75 function loss mutant (myb75-c), so that the anthocyanin accumulation amount of the transgenic plant reaches more than 15 times of that of a wild type control, and the leaves present a significant purplish red color. The application is particularly suitable for plant materials with limited anthocyanin synthesis capacity, and provides an innovative gene resource and technical scheme for efficient cultivation of crop and ornamental plant varieties with high anthocyanin content.

The invention discloses a pear transcription factor PyHAT5 and application of the pear transcription factor PyHAT5 in synthesis of red-flesh pear anthocyanin. The transcription factor PyHAT5 capable of regulating and controlling pulp anthocyanin biosynthesis in the red-pulp pears belongs to an HD-ZIP family member, the nucleotide sequence of the transcription factor PyHAT5 is as shown in SEQ ID No.1, and the amino acid sequence coded by the transcription factor PyHAT5 is as shown in SEQ ID No.2 in a sequence table. Through instantaneous overexpression in pear fruits, the PyHAT5 is proved to be capable of inhibiting accumulation of anthocyanin in the pear fruits; and the accumulation of the anthocyanin in the pear fruits can be promoted by instantaneously silencing the PyHAT5. The discovery of the gene supplements and perfects an anthocyanin synthesis regulation mechanism of HD-ZIP transcriptional regulation in pears, and the effect of the PyHAT5 gene on inhibiting accumulation of the flesh anthocyanin can be relieved through technologies such as gene editing and RNAi in the future, so that synthesis of the flesh anthocyanin of the pears is promoted, and a gene resource and a theoretical basis are provided for breeding of the red-flesh pears.

The invention belongs to the technical field of biology, and particularly relates to application of a lycium ruthenicum LrCHS1 gene and an encoded protein thereof in promotion of anthocyanin biosynthesis. It is found for the first time that the LrCHS1 gene can promote accumulation of anthocyanidin compounds and can be used for forward mediation of biosynthesis of fructus lycii anthocyanidin. By overexpressing the LrCHS1 gene in the plant, the anthocyanin can be obtained through a biosynthesis way, and a new method is provided for obtaining the anthocyanin. According to the construction method of the recombinant plasmid and the recombinant bacterium for overexpressing the lycium ruthenicum LrCHS1 gene, a recombinant expression vector for overexpressing the LrCHS1 gene can be obtained, and the obtained recombinant expression vector can improve the content of anthocyanin in plants and can be used for biosynthesis of the plants.

The application discloses a RsLNC2361 gene for regulating synthesis of radish anthocyanin and application thereof, and belongs to the technical field of genetic engineering.The application clones the RsLNC2361 gene from a purple skin and red flesh radish.The transient overexpression of the gene in radish leaves can increase the anthocyanin accumulation content in the radish leaves and significantly improve the expression level of anthocyanin biosynthesis related genes, and silencing of the RsLNC2361 in radish root can reduce the anthocyanin accumulation in the flesh root and the expression level of anthocyanin biosynthesis genes.The application not only helps to accelerate the radish breeding process and directionally cultivate a new radish variety with high anthocyanin content, but also provides an important theoretical basis for analyzing the molecular regulation mechanism of radish anthocyanin synthesis.

The invention discloses application of different transcripts of potato StB3-like in anthocyanin biosynthesis, and belongs to the technical field of biology. According to the invention, SMRT sequencing and RNA-seq technology are integrated, systematic research of three key development stages is carried out on three kinds of potatoes with different colors, and by analyzing a variable splicing mode of transcription factors, 33 variable splicing events are found, and StB3-like is a key gene of the invention. Experiments show that after co-expression with StAN1, StB3-like-2 and StB3-like-3 significantly increase the anthocyanin accumulation amount, while StB3-like-1 does not have the effect. Therefore, different transcripts of the same gene are proved, and anthocyanin accumulation is regulated and controlled through StAN1 dependency mechanism difference. The method disclosed by the invention has a very wide application prospect in the aspects of crop anthocyanin biosynthesis breeding and subsequent preparation of anthocyanin-containing health-care products.

Disclosed is use of an MdPHY7 protein or an MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of the apple anthocyanin biosynthesis, belonging to the technical field of plant genetic engineering. Exogenous 5-aminolevulinic acid (ALA) treatment can induce expression of the MdPHY7 gene, thereby promoting an increase of the apple anthocyanin biosynthesis, and then ultimately resulting in accumulation of apple anthocyanins to promote apple fruit coloring.

The invention discloses application of mango MiMYB37 in promotion of anthocyanin synthesis, provides an important theoretical basis and technical means for a molecular regulation mechanism of a mango anthocyanin biosynthesis pathway, has remarkable application potential in improvement of color and quality of mango fruits in agricultural production, and has important industrial popularization value. The gene is used for improving a mango variety and culturing the mango variety with high anthocyanin content, so that the economic benefit can be improved, and the high-quality development of the mango industry is promoted.

The invention discloses a dehydration response element binding protein FtDREB02 as well as a coding gene and application thereof. The dehydration response element binding protein FtDREB02 and the coding gene thereof are separated from tartary buckwheat through molecular cloning, the amino acid sequence of the dehydration response element binding protein FtDREB02 is shown as SEQ ID No.2, and the nucleotide sequence of the coding gene of the dehydration response element binding protein FtDREB02 is shown as SEQ ID No.1. Genetic transformation experiments prove that the encoding gene of the dehydration response element binding protein FtDREB02 can remarkably improve the plant drought tolerance, has the dual functions of enhancing the promoter activity of the anthocyanin synthase gene, and has application prospects in the aspects of improving the plant drought stress resistance or regulating the plant anthocyanin biosynthesis and the like.

The invention discloses a purple sweet potato anthocyanin synthesis regulatory factor IbACLA and application thereof. According to the invention, a purple sweet potato strain A5 is used as an experimental material, a promoter sequence of IbbHLH2 is cloned, and an upstream regulatory factor IbACLA of an IbbHLH2 gene is obtained through a yeast one-hybrid library screening experiment. A yeast single-hybridization rotary experiment and a dual-luciferase report system are used for detection to confirm that the IbbHLH2 promoter interacts with an upstream regulatory factor IbACLA. The basic theory of molecular regulation and control of plant anthocyanin biosynthesis can be enriched and deepened theoretically, and new thoughts and clues are expected to be provided for cultivation measures for improving the content of pigments in purple sweet potato tuberous roots.

The invention provides a polypeptide for promoting synthesis of plant anthocyanin and application, and belongs to the technical field of genetic engineering. The invention provides a polypeptide for promoting the synthesis of plant anthocyanin. The polypeptide is characterized in that a key site of a key structural domain for regulating and controlling the synthesis of the anthocyanin is mutated. According to the invention, the existing sequence is subjected to key site mutation and optimization, and is used for promoting the biosynthesis of anthocyanin. By mutating the key amino acid site of the original sequence, the regulation and control capability of the transcription factor in anthocyanin biosynthesis can be obviously improved. The invention also constructs an overexpression vector based on the coding gene or the optimized gene of the polypeptide, and the anthocyanin accumulation amount of a transgenic material can be obviously promoted by overexpressing the gene or the optimized gene in a plant cell.

A method for rapid verification of an anthocyanin biosynthesis gene function using Anthurium andraeanum ‘Xueyu’ is provided. An engineered bacterium carrying an anthocyanin biosynthesis-related functional gene AnUFGT1 from Anthurium andraeanum is injected into a young pedicel of the Anthurium andraeanum ‘Xueyu’ once a day for 3 consecutive times using a syringe. The anthocyanin biosynthesis on a white spathe is observed to verify a function of the AnUFGT1 gene in regulating the anthocyanin biosynthesis. The method has a simple process flow, including using a non-blossomed inflorescence of the Anthurium andraeanum ‘Xueyu’ as a material, injecting a bacterial suspension into the material, conducting routine management, and then observing a phenotype after the spathe is unfolded. The method also shows a short cycle taking 15 d to 20 d during the entire process. The method provides an efficient means for rapid verification of partial functional genes.

The invention belongs to the technical field of biology, and particularly relates to application of a chimonanthus praecox CpMADS1 gene in regulation and control of plant anthocyanin biosynthesis. The chimonanthus praecox CpMADS1 gene belongs to the MADS-box family, the expression of the chimonanthus praecox CpMADS1 gene is closely related to the formation of the flower color of the chimonanthus praecox, but the function of the chimonanthus praecox CpMADS1 gene in heterologous plants such as tobacco is not The chimonanthus praecox CpMADS1 gene is introduced into tobacco, and it is found that the chimonanthus praecox CpMADS1 gene significantly inhibits anthocyanin synthesis (the anthocyanin content of an OE-5 strain is 0.53 times that of a wild type) and accompanies slight down-regulation of the flavonoid content (the flavonoid content of an OE-9 strain is 0.82 times that of the wild type). Researches show that CpMADS1 may influence downstream metabolic flux distribution by regulating expression of key enzyme genes in an anthocyanin synthesis pathway, so that flower color phenotypes are changed. The invention provides a new strategy for regulating and controlling the synthesis of plant anthocyanin by using the MADS-box gene, and has important application value for flower breeding and pigment metabolism engineering.

The invention relates to the technical field of plant genetic engineering, and discloses application of a DkHOS15 gene in regulation of persimmon procyanidine biosynthesis, the nucleotide sequence of the DkHOS15 gene is as shown in SEQ ID NO.1, or is a sequence which is obtained by deleting, adding and / or substituting one or more nucleotides of the sequence as shown in SEQ ID NO.1 and still has a function of positively regulating the persimmon procyanidine biosynthesis. The invention proves that DkHOS15 is a positive regulation transcription factor gene synthesized by persimmon proanthocyanidins and has a function of promoting accumulation of persimmon proanthocyanidins. A key gene resource is provided for directional regulation and control of the persimmon procyanidine, and the gene has important significance in optimizing the tannin content of persimmon fruits, improving the quality of the persimmon fruits and promoting persimmon germplasm improvement work.