114 results about "Anthocyanin synthesis" patented technology

The invention provides an MYB transcription factor CmMYB6 implicated in chrysanthemum petal anthocyanin biosynthesis regulation. The coding region sequence of the MYB transcription factor CmMYB6 contains 765 nucleotides. A CmMYB6 amino acid sequence contains a conserved R2R3 MYB domain, a [D/E]Lx2[R/K]x3Lx6Lx3R motif and an ANDV motif exist in the R3 domain, the [D/E]Lx2[R/K]x3Lx6Lx3R motif can interact with bHLH, and the ANDV motif is the characteristic motif of the MYB transcription factor implicated in anthocyanin biosynthesis regulation. The gene expression of CmMYB6 rises in the petal growth process and is significantly and positively correlated with anthocyanin synthesis the CmMYB6 can induce the activity of synthesis of a key gene CmDFR promoter from anthocyanin, and the CmMYB6 has substantially enhanced induction usefulness and can strongly induce accumulation of tobacco leaf anthocyanin during cooperative expression of the CmMYB6 and MrbHLH1. The MYB transcription factor can be used in transcription regulation of plant anthocyanin biosynthesis and plant color modification.

The invention provides a method for cultivating tobacco rich in anthocyanin and an application thereof. The method comprises the following steps of recombining two confirmed genes, namely MrMYB1 and MrbHLH1 which can be used for regulating and controlling anthocyanin synthesis in red bayberry fruits, establishing an expression plasmid, screening genes capable of regulating and controlling anthocyanin synthesis by using a tobacco leaf transient expression technique, converting genes with confirmed functions into the tobacco, and cultivating tobacco rich in anthocyanin. The invention provides a method for cultivating tobacco rich in anthocyanin, so that health hazard caused by smoking is alleviated; and the method is also applicable to MYB and bHLH genes in other fruits besides red bayberry.

The invention relates to an anthocyanin synthesis related protein IbMYB113 as well as a coding gene and application thereof. The amino acid sequence of the IbMYB113 protein is as shown in SEQ ID NO: 2; the nucleotide sequence of the coding gene is shown as SEQ ID NO: 1. According to the invention, IbMYB113 protein and a coding gene thereof are cloned from red sweet potato peel, the IbMYB113 gene is connected to a plant expression vector, a transgenic tobacco plant over-expressing IbMYB113 is obtained through agrobacterium-mediated genetic transformation, pigment accumulation of the whole plant is shown, and the content of delphinidin and cornflower pigment is detected to be obviously increased by high performance liquid chromatography. The IbMYB113 protein and the coding gene thereof provided by the invention have important theoretical significance and application value for promoting synthesis and accumulation of plant anthocyanin.

The invention discloses a pear anthocyanin synthesis transcription factor PbMYB109 and an application thereof. The nucleotide sequence of the transcription factor is as shown in SEQ ID NO. 1, and theamino acid sequence of protein encoded by the transcription factor is as shown in SEQ ID NO. 2. By an agrobacterium-mediated genetic transformation method, pear fruits are transiently transformed andArabidopsis thaliana is stably transformed by a PbMYB109 gene, obtained transgenic plants are subjected to phenotype identification, results indicate that the transgenic Arabidopsis thaliana has obvious anthocyanin accumulation on rosette leaves, seed pods and main stems as compared with mild types, the clonal PbMYB109 gene is a new transcription factor participating in anthocyanin synthesis, andanthocyanin synthesis can be promoted. Discovery of the transcription factor provides an important gene resource for gene engineering of exploration of anthocyanin synthesis routes and improvement offruit quality.

An isolated or recombinant MYB polypeptide having activity as a transcription factor in the synthesis of proanthocyanidins in plants, and nucleic acid molecule encoding same, wherein the polypeptide activates in the plants (a) promoters of the leucoanthocyanidin (LAR) and anthocyanidid reductase (ANR) genes, and (b) promoters of at least two of the genes of the general flavonoid pathway. Use of the polypeptide and nucleic acid molecule in regulating the biosynthesis and accumulation of proanthocyanidins in plants, such as in modifying pasture quality of legumes, is also disclosed.

The invention discloses a peony PsMYB12 transcription factor as well as an encoding gene and application thereof. The peony PsMYB12 transcription factor is a protein of (a) or (b) which is described as follows: (a) a protein consists of an amino acid sequence shown by a sequence 1 in a sequence table; (b) proteins which are derived from (a), are associated with peony color spot formation and/or anthocyanin synthesis and are formed by replacing and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown by the sequence 1 in the sequence table. By means of VIGSsilencing and heterologous excess transformation analysis, the results show that the peony PsMYB12 transcription factor can regulate the expression of key enzyme genes in an anthocyanin synthesis route. The peony PsMYB12 transcription factor is speculated to play an important role in the synthesis and accumulation of anthocyanin in peony flower spots and the formation of color spots. The specifically expressed gene is cloned for the first time and the functions of the gene are verified, so that a foundation is laid for the molecular mechanism of the formation of the color spots, and technicalbasis and thought are provided for further analysis of the synthesis and regulation of flavonoids in peony flowers.

The invention discloses a strawberry glutathione-transferase FaGST gene, an express protein thereof and an application thereof, wherein the base sequence of the strawberry glutathione transferase FaGST gene is shown as SEQ ID NO. 1. The strawberry glutathione-transferase FaGST gene is cloned, and the FaGST1 is found to be related to the accumulation of anthocyanin in strawberry fruit through an infection, and after the gene is silenced, the expression amount is reduced, and the expression amount of main anthocyanin synthesis genes such as CHS1, F3H1, ANS1, UGT1 and the like are inhibited. Therefore, the FaGST gene will be widely used in anthocyanin synthesis.

The invention belongs to the technical field of agricultural biology, and particularly relates to a thellungiella salsuginea transcription factor EsMYB41 for controlling plant anthocyanin synthesis and an encoding gene and application thereof. The amino acid sequence of the thellungiella salsuginea transcription factor EsMYB41 for controlling plant anthocyanin synthesis is shown in SEQ ID No.1. Byconstructing an EsMyb41 containing over expression vector, tobacco and arabidopsis are transferred, and EsMYB41 transgenic tobacco and arabidopsis are obtained; results find that the transgenic planthas the almost whole-plant claret-colored phenotype; the anthocyanin content of different tissues of transgenic plants and the expression of the anthocyanin synthesis structure gene are both remarkably higher than the wild type, and meanwhile under the salt stress, the anti-oxidase capability of the transgenic plant is improved remarkably.

The invention relates to molecular biology and specifically discloses an R2R3-MYB transcription factor HbAn1 capable of positively regulating rubber tree anthocyanin synthesis and an encoding gene and application thereof. An amino acid sequence of the transcription factor HbAn1 is shown as SEQ ID NO.1, and a nucleotide sequence of the encoding gene is shown as SEQ ID NO.2. According to the invention, an encoding gene sequence of the R2R3-MYB transcription factor HbAn1 capable of regulating anthocyanin synthesis is separated from rubber trees for the first time, and functions and an application range of the R2R3-MYB transcription factor HbAn1 are determined. Researches and analysis find that the expression of HbAn1 is gradually reduced along with leaf senescence and anthocyanin fading; meanwhile, HbAn1 expression in dark-culture tender stems is higher than that in light-culture tender stems, and the HbAn1 expression has positive correlation with the anthocyanin content. The transcription factor can positively regulate rubber tree anthocyanin synthesis and achieves the purpose of increasing an anthocyanin accumulation amount through self overexpression.

The invention provides a chrysanthemum bHLH transcription factor (CmbHLH2) involved in anthocyanin biosynthesis and regulation. A nucleotide sequence of the chrysanthemum bHLH transcription factor is shown as SEQ: NO.1. When the CmbHLH2 and a CmMYB6 coordinate to perform instantaneous over-expression in tobacco leaves, anthocyanin accumulation can be strongly induced to change the original green color of the leaves into a red color. Therefore, by means of transgenosis, over-expression of the CmbHLH2 in a plant is achieved or expression of the CmbHLH2 in the chrysanthemum plant is inhibited so that anthocyanin synthesis enhanced and inhibited transgenic plants can be obtained respectively, and the colors and luster of the transgenic plants can change with anthocyanin synthesis change.

The invention belongs to the technical field of plant variety cultivation, and specifically relates to a phalaenopsis aphrodite R3-MYBx1 gene and application thereof in flower color adjustment. An open reading frame of the gene contains a 162bp base, and a base sequence of the gene is shown as SEQ ID NO.1. The MYBx1 gene participates in a petal coloration process, but the MYBx1 gene can express both white petals and red petals; phalaenopsis aphrodite petal coloration can be affected by a plurality of transcription factors, and the MYBx1 is one of regulation factors and participates in the petal coloration process. Application of the gene in overexpression in petunia shows that the gene is related to an anthocyanin content, and the stronger expression of the gene is, the stronger anthocyanin synthesis inhibition capacity is. The MYBx1 is the gene which is researched and shown for the first time to have an obvious and specific anthocyanin degrading function. Through overexpression in plants, the gene can create novel varieties with novel petal coloration characteristics.

The invention discloses a biological activator for promoting fruit coloring, and a preparation method and application thereof. Various nutrient substances, genistein and bioactive inducing substancesare dissolved in water or a potassium hydroxide solution respectively to prepare a uniform solution A, a uniform solution B and a uniform solution C, the three solutions are mixed with one another andundergo constant volume treatment to prepare the biological activator, and the preparation method is simple and rapid. The biological activator prepared in the invention contains nutrient substancessuch as nitrogen, phosphorus, potassium, boron, zinc and the like required by plant fruit development, also contains biological stimulation molecules used for promoting the formation of anthocyanin, and anthocyanin synthesis substrate induction factors used for promoting the formation of anthocyanin, and can promote anthocyanin synthesis, obviously improve the appearance color and coloring rate ofthe fruits and increase the weight and sweetness of the fruits, so the taste and quality of the fruits are improved; and the biological activator is safe, efficient, non-toxic, harmless and suitablefor planting of various plants.

The invention discloses a wild wheat blue grain character major gene as well as a molecular marker and application thereof. The gene is a wild wheat grain gene identified in a common wheat-wild wheat grain 4Ab (4B) substitution line, the gene is a major gene for regulating and controlling the wild wheat blue grain character, and anthocyanin synthesis can be regulated and controlled. The nucleotide sequence of the wild wheat blue grain major gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein of the major gene is shown as SEQ ID NO.2. The invention provides a basis for researching an anthocyanin synthesis mechanism of the wild wheat, and also provides a direction and a target spot for improving the content of anthocyanin by transforming plants through a genetic engineering means.

The invention discloses a potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 and application thereof, wherein a gene sequence of the StMYB44-1 is shown as SEQ ID NO.1. A preparationmethod of the potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 comprises the following steps of: selecting plant materials; selecting reagents; extracting RNA and qPCR; constructing an expression vector; and cloning the StMYB44-1 gene sequence according to a designed primer of a potato database, and obtaining a complete coding sequence of the StMYB44-1 from potato meat of a black beauty potato by using cDNA as a template through PCR amplification. The invention discloses the transcription factor StMYB44-1 for inhibiting the potato tuber anthocyanin synthesis at high temperature; and the potato tuber anthocyanin synthesis transcription inhibitor StMYB44-1 has important significance for clarifying a regulation and control mechanism of the potato tuber anthocyanin under the influence of temperature and improving the content of the potato anthocyanin on the basis of the regulation and control mechanism.

The invention discloses an application of a Paeonia lactiflora PlTDC gene in changing plant flower colors. The accession number of the Paeonia lactiflora PlTDC gene is KY765554. The constructed PlTDCgene overexpression vector is transformed into tobacco for expression, so that the anthocyanin content in plants, particularly in tobacco petals, is reduced, anthocyanin synthesis related gene expression is inhibited, and a novel light pink tobacco germplasm is created.

The invention discloses an MYB transcription inhibition factor LrMYB3 related to lycium ruthenicum anthocyanin synthesis and application thereof, and belongs to the technical field of gene engineering. According to transcriptome data of lycium ruthenicum, the MYB transcription inhibition factor LrMYB3 participating in anthocyanin synthesis is screened and cloned according to annotation results and expression quantity differences of MYB transcription factors, and belongs to a R2R3 type MYB transcription factor. Multiple sequence alignment and evolutionary tree analysis show that the transcription inhibition factor belongs to a FaMYB1-like transcription inhibition factor. QRT-PCR analysis shows that: the LrMYB3 is expressed in each tissue of the lycium ruthenicum, and the expression level is gradually increased along with ripening of the lycium ruthenicum. Subcellular localization and transcriptional activity detection experiments show that the LrMYB3 is a transcription factor which is localized in a cell nucleus and has no activation function.

The invention belongs to the field of wine making, and particularly relates to a methylpyrane anthocyanin preparation method and an application of methylpyrane anthocyanin prepared by the preparation method to the field of aged wine preparation. According to experimental determination, by the preparation method, after one month, the yield of the methylpyrane anthocyanin is relatively improved by 20%-40%. Therefore, synthesis of methylpyrane anthocyanin can be promoted, reaction time is shortened, the synthesis efficiency of the methylpyrane anthocyanin is improved on the whole, and the technical shortcomings of low synthesis speed and low synthesis yield of a methylpyrane anthocyanin synthesis method in the prior art are overcome.

The invention discloses a rapid plant phosphorus nutrition diagnosis and visual dynamic monitoring method and application of a recombinant expression vector. A promoter which specifically responds to a phosphorus deficiency signal is used for regulating and controlling a recombinant expression vector for plant anthocyanin synthetic route gene expression to be converted into a plant so as to obtain a transgenic plant; when sufficient phosphorus is supplied, the leaves of the transgenic plant are maintained to be original green; when the plant is lack of the phosphorus, the promoter specifically drives overexpression of the anthocyanin synthesis gene, the anthocyanin is greatly accumulated on the leaves of the transgenic plant, and then the leaves of the plant are turned into dark pulp; when sufficient phosphorus is supplied again, the transgenic plant can be recovered to be green within a short time, so that the purpose of visual dynamic monitoring of the phosphorus of the plant is achieved. Due to the adoption of the method, the dynamic change of the phosphorus inside the plant can be sensitively and specifically monitored, and meanwhile with the application of the remote sensing technique, the supply state of the plant phosphorus supply condition can be rapidly monitored in large area, so that instruction of rational application of fertilizers in the field can be realized.

The invention belongs to the field of genetic engineering in molecular biology, and specifically relates to a strawberry anthocyanin-related gene FvMYB17 and an application thereof. A nucleotide sequence of the FvMYB17 is shown in SEQ ID NO:1, and the amino acid sequence is shown in SEQ ID NO:2; the gene is indicated to have the function of promoting arabidopsis thaliana anthocyanin synthesis through research. A theoretical basis and technical means are provided for regulation of fruit color by using a genetic engineering technology, and great application value is achieved.

The invention relates to a method for improving the quality of grape fruits by using a compound anti-transpiration agent. In the period of fruit bearing, a hyaluronic acid solution is sprayed; from the initial period of maturation in the beginning to the initial period of maturation, the compound anti-transpiration agent is sprayed onto the surfaces of the fruits, hyaluronic acid can form an edible covered membrane on the surface of the grape skin, and the covered membrane maintains the local environmental humidity of the tissue while hindering the transpiration, thereby increasing the growthspeed of the grape fruits in the first expansion growth period, reducing the weight loss ratio of the fruits, reducing the water evaporation of the fruits, and enhancing the drought resistance ability. In the initial period of maturity of grapes in the beginning, ABA mixing treatment can obviously advance and improve the expression and expression quantity of a VvCWI (cell wall acid invertase) generelated to sugar accumulation and VvUFGT gene related to anthocyanin synthesis in the maturation process, and finally, the content of sugar, anthocyanin and other important quality factors in ripe grape fruits is increased.

The invention discloses an MYB transcription inhibition factor LrETC1 related to lycium ruthenicum anthocyanin synthesis and application thereof, and belongs to the technical field of gene engineering. According to the invention, the MYB transcription inhibition factor LrETC1 participating in the anthocyanin synthesis is screened and cloned, the total cDNA of the gene is 240bp, and 79 amino acids are encoded; protein sequence database analysis shows that the transcription factor belongs to R3 type MYB; and multiple sequence alignment and evolutionary tree analysis show that the transcription inhibition factor belongs to AtCPC-like transcription inhibition factors, and is a transcription factor which is located in a cell nucleus and does not have an activation function. A LrETC1 transgenic arabidopsis thaliana strain is obtained through agrobacterium tumefaciens-mediated genetic transformation, compared with a wild type, seed coats of seeds are light brown, arabidopsis thaliana seedlings are free of pigment accumulation under the stress condition of high sucrose concentration, and the expression level of related structural genes is remarkably reduced.