44 results about "Anthocyanin synthesis" patented technology

The invention discloses a method for regulating and controlling synthesis of tobacco flavonol and anthocyanin, a target gene and application of the target gene, and aims to solve the technical problem that biosynthesis of anthocyanin and flavonol of a current plant is not easy to regulate and control. According to the invention, a gene AFB capable of regulating and controlling tobacco biosynthesis is cloned from tobacco, an overexpression vector and a CRISPR / Cas9 vector of the gene AFB are constructed, and corresponding overexpression strains and gene knockout plants are respectively obtained through transformation; phenotype and physiological index determination shows that AFB belongs to an HD-ZIP IV family member, can regulate and control development of corolla conical epidermis cells and biosynthesis of plant anthocyanin and flavonol so as to control flower color, and has important application value in the aspects of flower color regulation and control, construction or screening of high-resistance and high-quality plant varieties and the like.

The invention relates to the technical field of gene engineering, and provides a lycium ruthenicum MYB transcription factor LrMYB77, the amino acid sequence of which is as shown in SEQ ID NO.3. The invention also provides a gene LrMYB77 for coding a transcription factor, and a recombinant expression vector and host bacteria containing the gene. Meanwhile, the invention further provides application of the transcription factor or the gene in promoting synthesis of lycium ruthenicum anthocyanin and a specific method for increasing the synthesis amount of the lycium ruthenicum anthocyanin. According to the invention, the LrMYB77 gene with a regulation effect on anthocyanin synthesis is screened from lycium ruthenicum for the first time, and experiments verify that the expression or overexpression of the gene in a plant is regulated, so that the synthesis and accumulation of anthocyanin in the plant can be remarkably promoted, and the LrMYB77 gene has important significance on germplasm resource creation and molecular breeding of lycium ruthenicum rich in anthocyanin.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a CebHLH113 gene for regulating and controlling synthesis of colocasia esculenta anthocyanin and application of the CebHLH113 gene. The amino acid sequence of the protein coded by the CebHLH113 gene is as shown in SEQ ID NO: 1. According to the invention, a key gene CebHLH113 for positively regulating and controlling anthocyanin synthesis is separated from red-pulp taro No. 3 of Ganxi taro for the first time. Overexpression of the CebHLH113 gene can significantly promote accumulation of anthocyanin in taro calluses, and silencing of the gene in stem tips significantly reduces the accumulation level of anthocyanin. The taros anthocyanin metabolism transcription regulation and control method fills the blank of a taros anthocyanin metabolism transcription regulation and control mechanism, is beneficial for providing important theoretical basis and technical support for improving the color and luster quality of taros by a molecular breeding technology and cultivating high-quality taros with high anthocyanin content, and has wide application value.

This invention relates to the field of plant genetic engineering technology, specifically providing a StTOE3 encoding gene, the StTOE3 gene, the StTOE3 protein, a recombinant vector, and their applications. The base sequence of the encoding gene is shown in SEQ ID NO.1. The StTOE3 encoding gene determines the function of its encoded protein in plants. Recombining this encoding gene with the pBvVA(V)KS vector template yielded the recombinant vector pBvVA(V)KS-StTOE3, which was overexpressed in tobacco plants. This demonstrated that overexpression of the StTOE3 gene increased the anthocyanin content in plants, indicating that the function of the protein encoded by the StTOE3 encoding gene is to increase the anthocyanin content, laying the foundation for further research on the specific role of the StTOE3 gene in the anthocyanin synthesis pathway.

The invention provides research on a mechanism for regulating red bayberry fruit anthocyanin synthesis through optical signals and application of the mechanism. By treating Dongkui waxberries with different light-transmitting bags, the quality characteristics of mature fruits are analyzed, and comprehensive analysis is performed in combination with transcriptome and metabolome. The result shows that the bagging in the young fruit period significantly reduces the fruit quality, the black light-proof bag inhibits the fruit color change and anthocyanin synthesis, and the bagging influence in the color change period is small. Metabolome and transcriptome analysis shows that differential metabolites are enriched in anthocyanin synthesis and flavonoid metabolic pathways, related differential metabolites are identified, and core regulation transcription factors HY5 and DF1 participate in the photoregulation anthocyanin accumulation process. According to the invention, an optical signal regulation and control mechanism is defined, related factors and metabolites are identified, and a basis is provided for quality improvement and cultivation technology optimization of waxberries.

This invention discloses the application of the PhZPT2-5 gene in regulating anthocyanin synthesis in petunias, belonging to the field of plant genetic engineering technology. This invention discovers that the PhZPT2-5 gene is significantly correlated with anthocyanin synthesis in petunias. Experiments show that silencing the PhZPT2-5 gene reduces anthocyanin synthesis, while overexpressing the PhZPT2-5 gene promotes anthocyanin synthesis. By regulating the expression of the PhZPT2-5 gene in petunias, different colored petunias can be obtained, laying the foundation for breeding new petunia varieties.

The application discloses an MYB transcription factor for changing the color of Paphiopedilum callosum and application of the MYB transcription factor. The nucleotide sequence of the transcription factor PpMYB1 is shown as SEQ ID NO. 1. The PpMYB1 gene is cloned from the petal of Paphiopedilum callosum, and is stably transformed into tobacco through an agrobacterium-mediated leaf disc method. The results show that overexpression of the PpMYB1 gene in tobacco makes the color of tobacco flowers deepen. The PpMYB1 gene is transformed into the petal of Paphiopedilum callosum through an agrobacterium-mediated transient expression technology. The results show that overexpression of the PpMYB1 gene in the petal of Paphiopedilum callosum makes the white petal present purplish red. It is proved that the PpMYB1 gene of Paphiopedilum callosum has the function of regulating the synthesis of anthocyanin of flowers.

This invention discloses a gene CsANS1 encoding anthocyanin synthase and its applications, belonging to the field of biotechnology. The nucleotide sequence of the gene CsANS1 is shown in SEQ ID NO.1. The gene CsANS1 or related biological materials can be used to synthesize anthocyanins and quercetin in vitro. This invention verifies through recombinant engineered bacteria that the anthocyanin synthase CsANS1 not only has the activity of catalyzing the conversion of colorless anthocyanins to anthocyanins, but also has a strong catalytic activity of catalyzing the conversion of quercetin, effectively overcoming the limitations of existing technologies in the production of anthocyanins and quercetin. The bifunctional catalytic properties of the gene CsANS1 broaden the application prospects of ANS enzymes in metabolic engineering and help promote the development and utilization of natural active ingredients in the fields of food, medicine, and cosmetics.

The invention discloses application of an AcMYB1 gene as a negative regulatory factor in regulation of synthesis of kiwi fruit anthocyanin, and belongs to the technical field of bioengineering. According to the invention, a key negative transcription factor AcMYB1 gene for regulating and controlling the coloring of kiwi fruits is found, and the key negative transcription factor AcMYB1 gene is clear as a negative regulation and control factor for anthocyanin synthesis. According to the discovery, a new key member is added for a regulation and control network of an important quality character of kiwi fruit color, and a candidate gene is provided for analyzing a formation mechanism of characteristic germplasm resources of red-pulp kiwi fruits.

The invention discloses a polianthes tuberose PtMYB86 gene and application thereof in regulation and control of flower blooming and anthocyanidin synthesis, and belongs to the technical field of molecular biology. The nucleotide sequence of the polianthes tuberose PtMYB86 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the polianthes tuberose PtMYB86 gene is as shown in SEQ ID NO. 2. The invention finds that the PtMYB86 gene has double functions in polianthes tuberosa: on one hand, the PtMYB86 gene is instantaneously over-expressed in polianthes tuberosa petals, so that the expansion of the petals can be obviously promoted, and the problem of'stiff flowers' that flower buds are difficult to open in the cultivation, production and transportation processes of cut flowers and potted flowers of the polianthes tuberosa is effectively solved; on the other hand, the color of the petals can be obviously changed by transient overexpression of the PtMYB86 gene in the petals of the tuberose, so that the flower color becomes light and white from red / pink, and evidence and a key target gene are provided for clarification of a molecular mechanism of dynamic change of the flower color of the pink variety of the tuberose.

The invention belongs to the technical field of plant biology, and discloses a BnaMYB73.C03 gene, a protein and application of the BnaMYB73.C03 gene and the protein in changing the flower color of brassica napus L. The nucleotide sequence of the BnaMYB73.C03 gene is shown as SEQ ID NO. 1. The BnaMYB73.C03 gene participates in the color generation process of rape petals, the gene is related to the anthocyanin content, and the stronger the expression is, the stronger the anthocyanin synthesis capability is, so that the BnaMYB73.C03 gene can be used for breeding a new variety of brassica napus. The research finds that the color of petals is deepened by over-expressing the BnaMYB73.C03 gene in the white rape. In addition, the invention also provides a corresponding transgenic breeding method, which provides a new molecular tool for rape breeding and flower color improvement, and has important ornamental value and economic benefit.

The invention discloses an application of MaC1 and MaLc genes in breeding of anthocyanin-rich fruit bananas. According to the method, external male bud pieces (dark red), male flower petals (light red), pericarp (cyan) and pulp (white) of wild bananas are used as materials, 25 genes on the variety, content and metabolic pathway of the anthocyanin are quantitatively analyzed, and the genes relate to genes related to synthesis, modification, transport and regulation of the anthocyanin. The key gene causing anthocyanin accumulation in bananas is found out by analyzing the anthocyanin metabolism difference at the four material taking parts, so that a basis is provided for subsequently creating new germplasm rich in anthocyanin. According to the method, MaC1 and MaLc genes of bananas are further started through a MaChIII promoter, whether the MaC1 and MaLc genes can change the color of banana fruits or not is verified through transient expression, new banana germplasm with the fruits rich in anthocyanin is successfully created through an agrobacterium tumefaciens-mediated banana genetic transformation system, the anthocyanin content of the banana germplasm is greatly increased compared with that of banana varieties in the market, and the method has the advantages that the method is simple and convenient to operate and has wide application prospects. The product is beneficial to human health.

The application belongs to the field of biotechnology, and particularly relates to application of a wax plant CpMADS1 gene in regulating biosynthesis of plant anthocyanins. The wax plant CpMADS1 gene belongs to the MADS-box family, and its expression is closely related to flower color formation of the wax plant, but its function in heterologous plants such as tobacco has not been revealed. In the application, the wax plant CpMADS1 gene is introduced into tobacco, and it is found that the gene significantly inhibits synthesis of anthocyanins, and is accompanied by slight down-regulation of flavonoid content. Research shows that CpMADS1 may affect downstream metabolic flux distribution by regulating expression of key enzyme genes in the anthocyanin synthesis pathway, so as to change the flower color phenotype. The application provides a new strategy for regulating plant anthocyanin synthesis by using MADS-box genes, and has important application value for flower breeding and pigment metabolism engineering.

The application provides application of MdPHY7 protein or MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of apple anthocyanin synthesis, and belongs to the technical field of plant genetic engineering. Exogenous ALA treatment can induce MdPHY7 gene expression, thereby promoting anthocyanin synthesis increase, finally causing apple anthocyanin accumulation and promoting fruit coloring. It has been verified that overexpression of MdPHY7 gene can promote apple anthocyanin accumulation, while interference with MdPHY7 gene expression can inhibit anthocyanin accumulation and further inhibit fruit appearance quality improvement. It can be seen that MdPHY7 is a new key gene for regulating apple anthocyanin accumulation, and plays a key role in ALA regulation of apple anthocyanin accumulation, which has far-reaching significance for agricultural and forestry production, and lays a solid theoretical foundation for application of ALA in the field of agricultural production.

The invention discloses recombinant escherichia coli with high yield of cyanidin-3-O-glucoside as well as a construction method and application of the recombinant escherichia coli. The method comprises the following steps: by taking escherichia coli BL21 (DE3) engineering bacteria as a host, integrating flavanonol 4-reductase, anthocyanin synthetase and flavonoid 3-O-glucosyltransferase from different sources onto a plasmid vector pRSFDuet, and constructing to obtain a chassis cell for producing C3G; meanwhile, glutathione transferase is overexpressed, dissolution-promoting tag small ubiquitin-like modified protein is added, FaDFR is modified through enzyme engineering, FaDFR catalytic efficiency is improved through artificial protein cage space combination pathway enzyme, key precursor UDP-glucose is synthesized in an in-situ intensified mode and cofactor engineering, the cell factory for efficiently synthesizing C3G is constructed based on the combination strategy, the yield of C3G is increased, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. The titer reaches 797.20 mg / L. The method provided by the invention has the characteristics of simplicity, economy and eco-friendliness.

The application belongs to the technical field of genetic engineering, and provides a gene OsMYB1 and application of the gene in negative regulation of anthocyanin synthesis; the cDNA sequence of the gene OsMYB1 is shown as SEQ ID NO. 1. The application proves by systematic molecular biology experiments that OsMYB1 can inhibit the expression of OsDFR by combining with the promoter region of the key structural gene OsDFR of anthocyanin synthesis, thereby inhibiting the synthesis of anthocyanin. Meanwhile, OsMYB1 can also form an MBW ternary complex with OsB2 and OsPAC1, competitively inhibits the synthesis of anthocyanin, and indicates that the transcription factor OsMYB1 is a negative regulation factor of anthocyanin synthesis in rice, and knocking out the same can increase the content of anthocyanin in rice, thereby providing certain theoretical basis for cultivating high-quality purple rice with high anthocyanin content.

The invention discloses a method for increasing the anthocyanin content of aizoon stonecrop herb cells through sectional treatment, and belongs to the technical field of plant cell engineering. According to the method, dopamine hydrochloride is applied to induction regulation and control of plant cell anthocyanin synthesis for the first time, and methyl jasmonate and dopamine hydrochloride are sequentially added in two stages according to metabolism time sequence characteristics of aizoon stonecrop herb cells in the logarithmic phase: in the first stage, 20-50 [mu] mol / L of methyl jasmonate is added on the first day of the logarithmic phase, and 20-50 [mu] mol / L of dopamine hydrochloride is added on the second day of the logarithmic phase; activating a phenylpropane metabolic pathway in a targeted manner to synthesize an anthocyanin precursor; in the second stage, 5-25 [mu] mol / L of dopamine hydrochloride is added 24-48 h after the pathway is activated, active oxygen is removed, and carbon source distribution is directionally optimized. According to the method, the problem of action time sequence mismatching of traditional one-time compounding is solved, the anthocyanin content is increased by 90% or above, the problem that only the content can be increased but the browning rate is too high when single methyl jasmonate serves as an inducer is solved, the cell browning rate is lower than 3%, and the method is suitable for industrial large-scale production and has remarkable economic value and application prospects.

The invention provides a gene AcANR1 for regulating and controlling the accumulation of proanthocyanidins in kiwi fruits. The nucleotide sequence of the gene AcANR1 is shown as SEQ. ID. NO. 1. The invention also provides a recombinant expression vector containing the gene, a recombinant agrobacterium and application of the gene in improvement of plant procyanidine accumulation and cultivation of high-quality kiwi fruit. The proanthocyanidins anabolism regulation gene AcANR1 is screened from kiwi fruits by combining kiwi fruit genome and transcriptome database expression analysis, and the accumulation amount of proanthocyanidins in plants can be remarkably increased through overexpression of the gene, so that the proanthocyanidins anabolism regulation gene AcANR1 is obtained. The method has important theoretical significance and practical application value on analysis of a kiwi fruit procyanidine synthesis regulation molecular mechanism, breeding of high-quality kiwi fruit varieties and kiwi fruit quality improvement.

The application discloses a radish RsMYB308 gene and application thereof, and relates to the technical field of plant genes.The radish RsMYB308 gene is obtained by a homologous cloning method, and overexpression vectors and silencing vectors of the RsMYB308 gene are respectively transformed into radish leaves and red-fleshed red-skin radishes by an agrobacterium-mediated method.The results show that the expression of genes related to anthocyanin synthesis in the radish leaves with overexpression of the RsMYB308 gene is significantly increased, and the anthocyanin content is obviously higher than that of a control group, the RsMYB308 gene can increase the anthocyanin content in the radish leaves and promote anthocyanin accumulation, the expression of genes related to anthocyanin synthesis in the radish root flesh with silencing expression of the RsMYB308 gene is down-regulated, and the anthocyanin content is obviously lower than that of the control group.The RsMYB308 gene provided by the application can accelerate the breeding process of the radish, rapidly cultivate radish varieties with high anthocyanin content, and provide a theoretical basis for analyzing anthocyanin synthesis of the radish.

This invention discloses the use of SmDREB1D in increasing anthocyanin content in plants, belonging to the field of biotechnology. This invention is the first to experimentally demonstrate that the protein SmDREB1D and its encoding gene provided by this invention can increase the anthocyanin content of plants: compared with the eggplant variety Africa eggplant, the total anthocyanin content in the stems and floral organs of T1 generation eggplants transgenic with the SmDREB1D gene is significantly increased. Therefore, the protein SmDREB1D and its encoding gene have important theoretical significance and practical value in regulating anthocyanin synthesis in plants.

Provided is a polypeptide for promoting plant anthocyanin synthesis, comprising a polypeptide obtained by mutating a key site in a key domain for regulating anthocyanin synthesis. By performing key-site mutation and optimization on an existing sequence, and using the resulting polypeptide to promote anthocyanin biosynthesis, it has been verified that the polypeptide can significantly improve the regulatory ability of a transcription factor in the anthocyanin biosynthesis. An overexpression vector is also constructed on the basis of a coding gene or optimized gene for the polypeptide. By overexpressing the gene or optimized gene in a plant cell, anthocyanin accumulation in a transgenic material can be significantly promoted.

The application discloses application of NcolMYB75-like gene of blue star water lily in improving anthocyanin content of plants and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the NcolMYB75-like gene provided in the application is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. The application finds that the gene can strongly promote anthocyanin biosynthesis by heterologous expression of the gene in Arabidopsis thaliana AtMYB75 function loss mutant (myb75-c), so that the anthocyanin accumulation amount of the transgenic plant reaches more than 15 times of that of a wild type control, and the leaves present a significant purplish red color. The application is particularly suitable for plant materials with limited anthocyanin synthesis capacity, and provides an innovative gene resource and technical scheme for efficient cultivation of crop and ornamental plant varieties with high anthocyanin content.

The invention discloses a pear transcription factor PyHAT5 and application of the pear transcription factor PyHAT5 in synthesis of red-flesh pear anthocyanin. The transcription factor PyHAT5 capable of regulating and controlling pulp anthocyanin biosynthesis in the red-pulp pears belongs to an HD-ZIP family member, the nucleotide sequence of the transcription factor PyHAT5 is as shown in SEQ ID No.1, and the amino acid sequence coded by the transcription factor PyHAT5 is as shown in SEQ ID No.2 in a sequence table. Through instantaneous overexpression in pear fruits, the PyHAT5 is proved to be capable of inhibiting accumulation of anthocyanin in the pear fruits; and the accumulation of the anthocyanin in the pear fruits can be promoted by instantaneously silencing the PyHAT5. The discovery of the gene supplements and perfects an anthocyanin synthesis regulation mechanism of HD-ZIP transcriptional regulation in pears, and the effect of the PyHAT5 gene on inhibiting accumulation of the flesh anthocyanin can be relieved through technologies such as gene editing and RNAi in the future, so that synthesis of the flesh anthocyanin of the pears is promoted, and a gene resource and a theoretical basis are provided for breeding of the red-flesh pears.

The present application relates to the technical field of genetic engineering, and particularly relates to application of VvbHLH68 gene and VvERF104 gene in regulation of anthocyanin synthesis in grape. The VvbHLH68 gene transcription factor is subcellularly located in the nucleus, and specific interaction exists between the VvbHLH68 gene and the VvERF104 gene, which is verified by yeast two-hybrid and luciferase complementation assay. The co-transformation test shows that the VvbHLH68 gene and the VvERF104 gene can significantly enhance the anthocyanin and flavonoid synthesis capacity of grape callus in synergistic action.

The present application belongs to the technical field of bioengineering, and particularly relates to a gene ChANS encoding anthocyanin synthetase and application thereof, wherein a nucleotide sequence of the ChANS gene is shown as SEQ ID NO. 1. The anthocyanin synthetase gene ChANS is obtained by cloning and preparation from a Cerasus humilis plant. The anthocyanin synthetase of the Cerasus humilis is a key functional enzyme of an anthocyanin synthesis pathway of the Cerasus humilis, and can effectively regulate anthocyanin content and color formation process of fruits. Based on the ChANS gene provided by the present application, the content of functional ingredients anthocyanin in the Cerasus humilis fruits is directedly improved through a genetic engineering technical means, and efficient synthesis and enrichment of the anthocyanin are promoted. The present application provides important gene resources and technical support for preparation of anthocyanin functional compounds of the Cerasus humilis, improvement of Cerasus humilis germplasm resources and optimization and upgrading of fruit color quality, and has high scientific research value and application prospect.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a transcription factor MdWRKY71 or a MdWRKY71 gene in promoting anthocyanin accumulation in plants, wherein an amino acid sequence of the transcription factor MdWRKY71 is shown as SEQ ID NO:1, and a nucleotide sequence of the MdWRKY71 gene is shown as SEQ ID NO:2. The transcription factor MdWRKY71 is synthesized by the MdWRKY71 gene, and it is proved by transgenic and functional identification that the transcription factor MdWRKY71 synthesized by the MdWRKY71 gene as a new transcription factor for regulating anthocyanin accumulation in apples can positively regulate expression of key anthocyanin synthesis enzyme genes MdANS and MdDFR, and promote 5-amino levulinic acid (ALA)-induced anthocyanin accumulation in apples, and can play an important role in agricultural and forestry production and scientific research.

The application discloses a RsLNC2361 gene for regulating synthesis of radish anthocyanin and application thereof, and belongs to the technical field of genetic engineering.The application clones the RsLNC2361 gene from a purple skin and red flesh radish.The transient overexpression of the gene in radish leaves can increase the anthocyanin accumulation content in the radish leaves and significantly improve the expression level of anthocyanin biosynthesis related genes, and silencing of the RsLNC2361 in radish root can reduce the anthocyanin accumulation in the flesh root and the expression level of anthocyanin biosynthesis genes.The application not only helps to accelerate the radish breeding process and directionally cultivate a new radish variety with high anthocyanin content, but also provides an important theoretical basis for analyzing the molecular regulation mechanism of radish anthocyanin synthesis.

The present application belongs to the field of plant molecular biology and genetic engineering technology, and particularly relates to a lily anthocyanin synthesis regulation gene LvWRKY75 and application thereof, and the present application has obtained an overexpression vector and a silencing expression vector of the gene. The overexpression LvWRKY75 gene is transformed into wild-type Arabidopsis, and after obtaining the third generation of transgenic plants, the expression amount of the LvWRKY75 gene and downstream target genes in the transgenic lines is detected, and it is proved that the LvWRKY75 gene has the effect of promoting anthocyanin synthesis. The yeast one-hybrid technology, gel retardation experiment and double-molecule fluorescence complementation experiment prove that the LvWRKY75 gene plays a positive regulation role in the regulation of Westin red lily color, and these findings provide important experimental and theoretical basis for further understanding the molecular mechanism and genetic regulation network of plant flower color formation.