79 results about "Anthocyanin synthesis" patented technology

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention discloses a key gene AaMYB114 closely linked with the color character of an actinidia arguta fruit and application of the key gene AaMYB114. The AaMYB114 gene disclosed by the invention is positioned at 5237694-5239966bp of a chromosome 9 of an actinidia arguta genome, and the full length of the AaMYB114 gene is 2273bp. According to the application, all-red type and all-green type actinidia arguta are used as materials for transcriptome sequencing, and the gene AaMYB114 related to the formation of the peel color of the actinidia arguta is explored. Instantaneous conversion of the actinidia arguta fruit proves that the overexpression of the AaMYB114 increases the anthocyanin content in the actinidia arguta peel. Yeast one-hybridization and dual-luciferase report tests prove that the AaMYB114 not only interacts with a promoter of AaGST, activates the expression of the promoter and regulates anthocyanin transport of peel tissues of actinidia arguta, but also is combined with promoters of anthocyanin synthesis structural genes AaPAL, AaCHI and AaF3H and activates the expression of the promoters to promote anthocyanin synthesis. Therefore, the AaMYB114 gene plays a dual regulation and control role in the formation of peel color and luster, and can be used for cultivating red-peel actinidia arguta.

The invention relates to the technical field of molecular breeding, and discloses a functional marker development method based on a rice anthocyanin synthesis key gene OsTTG1 and application of the functional marker development method in molecular assisted breeding. The marker comprises a specific primer composition: a forward primer as shown in SEQ ID NO: 1, a reverse primer (used for specifically amplifying an A allele) with the 3'end being AT 'as shown in SEQ ID NO: 2, a reverse primer (used for specifically amplifying a G allele) with the 3' end being AC 'as shown in SEQ ID NO: 3, and universal fluorescent primers as shown in SEQ ID NO: 4 and SEQ ID NO: 5. The invention further provides a kit containing the primer composition, a method for carrying out fluorescent PCR typing by utilizing a PARMS technology and application of the kit in screening high-anthocyanin rice germplasm. The marker is used for directly detecting a functional site, the verification accuracy rate in 419 germplasm reaches 99.05%, the breeding efficiency can be remarkably improved, and the breeding period can be shortened.

The invention discloses a gene CsANS1 for coding anthocyanin synthetase and application of the gene CsANS1, and belongs to the technical field of biology. The nucleotide sequence of the gene CsANS1 is as shown in SEQ ID NO. 1. The gene CsANS1 or related biological materials thereof can be used for synthesizing anthocyanin and quercetin in vitro. Recombinant engineering bacteria verify that the anthocyanin synthetase CsANS1 not only has activity of catalyzing colorless anthocyanin to generate anthocyanin, but also has relatively strong activity of catalyzing quercetin to generate, so that the limitation of anthocyanin and quercetin production in the prior art is effectively solved. The bifunctional catalytic characteristic of the gene CsANS1 widens the application prospect of ANS enzyme in metabolic engineering, and is helpful for promoting the development and utilization of natural active ingredients in the fields of food, medicine, cosmetics and the like.

The invention discloses a method for regulating and controlling synthesis of tobacco flavonol and anthocyanin, a target gene and application of the target gene, and aims to solve the technical problem that biosynthesis of anthocyanin and flavonol of a current plant is not easy to regulate and control. According to the invention, a gene AFB capable of regulating and controlling tobacco biosynthesis is cloned from tobacco, an overexpression vector and a CRISPR / Cas9 vector of the gene AFB are constructed, and corresponding overexpression strains and gene knockout plants are respectively obtained through transformation; phenotype and physiological index determination shows that AFB belongs to an HD-ZIP IV family member, can regulate and control development of corolla conical epidermis cells and biosynthesis of plant anthocyanin and flavonol so as to control flower color, and has important application value in the aspects of flower color regulation and control, construction or screening of high-resistance and high-quality plant varieties and the like.

The invention relates to the technical field of gene engineering, and provides a lycium ruthenicum MYB transcription factor LrMYB77, the amino acid sequence of which is as shown in SEQ ID NO.3. The invention also provides a gene LrMYB77 for coding a transcription factor, and a recombinant expression vector and host bacteria containing the gene. Meanwhile, the invention further provides application of the transcription factor or the gene in promoting synthesis of lycium ruthenicum anthocyanin and a specific method for increasing the synthesis amount of the lycium ruthenicum anthocyanin. According to the invention, the LrMYB77 gene with a regulation effect on anthocyanin synthesis is screened from lycium ruthenicum for the first time, and experiments verify that the expression or overexpression of the gene in a plant is regulated, so that the synthesis and accumulation of anthocyanin in the plant can be remarkably promoted, and the LrMYB77 gene has important significance on germplasm resource creation and molecular breeding of lycium ruthenicum rich in anthocyanin.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a CebHLH113 gene for regulating and controlling synthesis of colocasia esculenta anthocyanin and application of the CebHLH113 gene. The amino acid sequence of the protein coded by the CebHLH113 gene is as shown in SEQ ID NO: 1. According to the invention, a key gene CebHLH113 for positively regulating and controlling anthocyanin synthesis is separated from red-pulp taro No. 3 of Ganxi taro for the first time. Overexpression of the CebHLH113 gene can significantly promote accumulation of anthocyanin in taro calluses, and silencing of the gene in stem tips significantly reduces the accumulation level of anthocyanin. The taros anthocyanin metabolism transcription regulation and control method fills the blank of a taros anthocyanin metabolism transcription regulation and control mechanism, is beneficial for providing important theoretical basis and technical support for improving the color and luster quality of taros by a molecular breeding technology and cultivating high-quality taros with high anthocyanin content, and has wide application value.

This invention discloses a wheat blue-grain gene and its applications, belonging to the fields of plant molecular biology, biochemistry, genetics, and plant breeding. Using differential expression analysis between blue-grain and white-grain wheat, four genes controlling the blue-grain trait were identified: two MYB family transcription factors and two bHLH family transcription factors. Furthermore, plant recombinant expression vectors for these genes and a method for regulating anthocyanin synthesis in plants are provided. This has significant theoretical and practical implications for studying the aleurone layer pigment synthesis pathway in blue-grain wheat, its use as a screening marker in plant transformation processes, and improving the nutritional value of plants.

The invention discloses a pink asparagus creation method, and relates to the technical field of asparagus planting, the pink asparagus creation method comprises the following steps: 1, variety selection: selecting a purple asparagus variety with stable anthocyanin synthesis and strong heat resistance; step 2, cultivation management; a conventional greenhouse and a small rectangular arched shed are arranged and covered with a black and white mulching film with the light transmittance being 0 and the thickness being 8-12 threads, in the initial state, the black film face is upward, the white film face is downward, the film edge is compacted, and drip irrigation belts are arranged between ditches; 4, light and temperature regulation and control: when the day and night temperature in the greenhouse is continuously 15-28 DEG C, the black and white mulching film is turned over to enable the white film to face upwards and the black film to face downwards; step 5, harvesting management; and step 6, keeping the mother stems for breeding. According to the method, illumination and temperature control is decoupled through the external greenhouse and the internal small arched shed, and film surface overturning and micropore design are combined, so that regulation and control of illumination and temperature of the asparagus in the small arched shed are achieved, and pink expression of the purple asparagus in the small arched shed is kept.

This invention relates to the field of plant genetic engineering technology, specifically providing a StTOE3 encoding gene, the StTOE3 gene, the StTOE3 protein, a recombinant vector, and their applications. The base sequence of the encoding gene is shown in SEQ ID NO.1. The StTOE3 encoding gene determines the function of its encoded protein in plants. Recombining this encoding gene with the pBvVA(V)KS vector template yielded the recombinant vector pBvVA(V)KS-StTOE3, which was overexpressed in tobacco plants. This demonstrated that overexpression of the StTOE3 gene increased the anthocyanin content in plants, indicating that the function of the protein encoded by the StTOE3 encoding gene is to increase the anthocyanin content, laying the foundation for further research on the specific role of the StTOE3 gene in the anthocyanin synthesis pathway.

The invention discloses a gene IsMYBL2 derived from phallus impudicus petals. The invention provides an IsMYBL2 gene for promoting anthocyanin synthesis of phallus impudicus and application of the IsMYBL2 gene. The nucleotide sequence of the gene IsMYBL2 is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the gene IsMYBL2 is as shown in SEQ ID NO. 2. A VIGS silencing system is constructed in the phallus impudicus floral part, it is found for the first time that accumulation of anthocyanin can be promoted through transient expression of IsMYBL2 in the phallus impudicus leaf base part, and flower color can become light through silencing of IsMYBL2 in the phallus impudicus floral part by using a VIGS technology. The overexpression of the IsMYBL2 gene can deepen the colors of tobacco leaves, stems, flowers and other positions. The IsMYBL2 gene provided by the invention not only provides a theoretical reference for research on flower color regulation and control of phallus impudicus, but also provides an important gene resource for flower color breeding work in the future.

The invention provides research on a mechanism for regulating red bayberry fruit anthocyanin synthesis through optical signals and application of the mechanism. By treating Dongkui waxberries with different light-transmitting bags, the quality characteristics of mature fruits are analyzed, and comprehensive analysis is performed in combination with transcriptome and metabolome. The result shows that the bagging in the young fruit period significantly reduces the fruit quality, the black light-proof bag inhibits the fruit color change and anthocyanin synthesis, and the bagging influence in the color change period is small. Metabolome and transcriptome analysis shows that differential metabolites are enriched in anthocyanin synthesis and flavonoid metabolic pathways, related differential metabolites are identified, and core regulation transcription factors HY5 and DF1 participate in the photoregulation anthocyanin accumulation process. According to the invention, an optical signal regulation and control mechanism is defined, related factors and metabolites are identified, and a basis is provided for quality improvement and cultivation technology optimization of waxberries.

This invention discloses the application of the PhZPT2-5 gene in regulating anthocyanin synthesis in petunias, belonging to the field of plant genetic engineering technology. This invention discovers that the PhZPT2-5 gene is significantly correlated with anthocyanin synthesis in petunias. Experiments show that silencing the PhZPT2-5 gene reduces anthocyanin synthesis, while overexpressing the PhZPT2-5 gene promotes anthocyanin synthesis. By regulating the expression of the PhZPT2-5 gene in petunias, different colored petunias can be obtained, laying the foundation for breeding new petunia varieties.

The invention belongs to the technical field of begonia new variety cultivation, and particularly relates to application of begonia sempervirens BsMYB15 in anthocyanin regulation and control. The nucleotide sequence of the begonia sempervirens BsMYB15 gene is as shown in SEQ ID No. 1. According to the application, on the basis of preliminary research on begonia sempervirens transcriptome in the earlier stage, further screening is carried out to obtain the BsMYB15 gene related to anthocyanin regulation, and the preliminary research result of the gene shows that the gene is combined with a BsDFR promoter to mediate the change of the anthocyanin content, and meanwhile, the gene also participates in anthocyanin synthesis regulation through interaction with BsDREB1B. A direct gene expression verification result shows that after overexpression, accumulation of anthocyanin can be obviously promoted, and after silence, the anthocyanin content can be correspondingly reduced. Based on the results, a certain technical foundation can be laid for the cultivation of new varieties of begonia in four seasons and the cultivation of new varieties of other plants.

The application discloses an MYB transcription factor for changing the color of Paphiopedilum callosum and application of the MYB transcription factor. The nucleotide sequence of the transcription factor PpMYB1 is shown as SEQ ID NO. 1. The PpMYB1 gene is cloned from the petal of Paphiopedilum callosum, and is stably transformed into tobacco through an agrobacterium-mediated leaf disc method. The results show that overexpression of the PpMYB1 gene in tobacco makes the color of tobacco flowers deepen. The PpMYB1 gene is transformed into the petal of Paphiopedilum callosum through an agrobacterium-mediated transient expression technology. The results show that overexpression of the PpMYB1 gene in the petal of Paphiopedilum callosum makes the white petal present purplish red. It is proved that the PpMYB1 gene of Paphiopedilum callosum has the function of regulating the synthesis of anthocyanin of flowers.

This invention discloses a gene CsANS1 encoding anthocyanin synthase and its applications, belonging to the field of biotechnology. The nucleotide sequence of the gene CsANS1 is shown in SEQ ID NO.1. The gene CsANS1 or related biological materials can be used to synthesize anthocyanins and quercetin in vitro. This invention verifies through recombinant engineered bacteria that the anthocyanin synthase CsANS1 not only has the activity of catalyzing the conversion of colorless anthocyanins to anthocyanins, but also has a strong catalytic activity of catalyzing the conversion of quercetin, effectively overcoming the limitations of existing technologies in the production of anthocyanins and quercetin. The bifunctional catalytic properties of the gene CsANS1 broaden the application prospects of ANS enzymes in metabolic engineering and help promote the development and utilization of natural active ingredients in the fields of food, medicine, and cosmetics.

The invention discloses application of an AcMYB1 gene as a negative regulatory factor in regulation of synthesis of kiwi fruit anthocyanin, and belongs to the technical field of bioengineering. According to the invention, a key negative transcription factor AcMYB1 gene for regulating and controlling the coloring of kiwi fruits is found, and the key negative transcription factor AcMYB1 gene is clear as a negative regulation and control factor for anthocyanin synthesis. According to the discovery, a new key member is added for a regulation and control network of an important quality character of kiwi fruit color, and a candidate gene is provided for analyzing a formation mechanism of characteristic germplasm resources of red-pulp kiwi fruits.

This invention discloses the application of the apple C2H2 zinc finger protein transcription factor MdZAT1 gene in anthocyanin synthesis, belonging to the field of plant genetic engineering technology. This invention isolates a C2H2 zinc finger protein transcription factor MdZAT1 gene from apples and experimentally reveals a negative correlation between MdZAT1 gene expression and anthocyanin content in apple fruit during development. Overexpression of MdZAT1 in apple callus tissue inhibits anthocyanin synthesis; transient overexpression and silencing experiments in apple peel show that MdZAT1 is a negative regulator of anthocyanin accumulation in apple peel. Yeast one-hybrid assays, EMSA, and dual-luciferase assays verify that MdZAT1 protein binds to and negatively regulates MdMYB114, MdCHI, and MdANS. The MdZAT1 gene has practical application value in regulating anthocyanins in apple fruit, providing a basis for improving the appearance and quality of apple fruit.

The invention discloses a polianthes tuberose PtMYB86 gene and application thereof in regulation and control of flower blooming and anthocyanidin synthesis, and belongs to the technical field of molecular biology. The nucleotide sequence of the polianthes tuberose PtMYB86 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the polianthes tuberose PtMYB86 gene is as shown in SEQ ID NO. 2. The invention finds that the PtMYB86 gene has double functions in polianthes tuberosa: on one hand, the PtMYB86 gene is instantaneously over-expressed in polianthes tuberosa petals, so that the expansion of the petals can be obviously promoted, and the problem of'stiff flowers' that flower buds are difficult to open in the cultivation, production and transportation processes of cut flowers and potted flowers of the polianthes tuberosa is effectively solved; on the other hand, the color of the petals can be obviously changed by transient overexpression of the PtMYB86 gene in the petals of the tuberose, so that the flower color becomes light and white from red / pink, and evidence and a key target gene are provided for clarification of a molecular mechanism of dynamic change of the flower color of the pink variety of the tuberose.

The present invention relates to StHY5 The application of genes in the synthesis of potato anthocyanins belongs to the field of genetic engineering technology. StHY5 Genes were obtained by screening differentially expressed genes in anthocyanin synthesis-related transcriptomes. StHY5 The expression pattern of this gene in potato tubers of different phenotypes was found to be correlated with the anthocyanin content in the tubers. By constructing an overexpression vector, overexpression in tobacco can promote the synthesis of anthocyanin in tobacco leaves, indicating that potato StHY5 The gene plays an important role in the synthesis of anthocyanins. StHY5 Identifying the gene and its role in potato anthocyanin synthesis is of great significance for breeding new potato varieties with high anthocyanin content.

The application provides application of PavMYB1 gene in regulation of anthocyanin synthesis of sweet cherry. The nucleotide sequence of the PavMYB1 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The application provides an overexpression vector and a VIGS gene silencing vector constructed by related genes and a construction method thereof, and a recombinant microorganism containing the vectors. The application proves through experiments that overexpression of PavMYB1 can increase pigment accumulation and expression of key anthocyanin synthesis genes of fruits, provides technical support and theoretical basis for improvement of fruit quality of sweet cherry, and helps to reveal the regulation mechanism of anthocyanin synthesis of sweet cherry.

The invention belongs to the field of gene engineering, and relates to a gene combination for synthesizing delphinidin, a recombinant plasmid combination, a strain and a method for heterologous biosynthesis of delphinidin. The gene combination comprises the following genes: a flavanone 3-hydroxylase gene TbF3H, a P450 cytochrome oxidase gene TbCYP, a flavanonol 4-reductase TbDFR, an anthocyanin synthetase TbANS and a P450 cytochrome reductase gene LjCPR, and the flavanone 3-hydroxylase gene TbF3H, the P450 cytochrome oxidase gene TbCYP, the flavanonol 4-reductase TbDFR, the anthocyanin synthetase TbANS and the P450 cytochrome reductase gene The invention provides a potential method for replacing traditional artificial cultivation or submerged fermentation acquisition, and lays a foundation for further transforming efficient biosynthesis through metabolic engineering.

The invention discloses an IdF3H gene derived from belamcanda chinensis petals. The invention provides an IdF3H gene capable of promoting anthocyanin synthesis. An overexpression vector of the IdF3H gene is constructed, the overexpression vector is transferred into wild tobacco through agrobacterium-mediated genetic transformation, and the anthocyanin content of transgenic tobacco can be obviously increased. The IdF3H gene can be applied to genetic engineering genetic breeding of plants and cultivation of safflower varieties.

The invention belongs to the technical field of plant biology, and discloses a BnaMYB73.C03 gene, a protein and application of the BnaMYB73.C03 gene and the protein in changing the flower color of brassica napus L. The nucleotide sequence of the BnaMYB73.C03 gene is shown as SEQ ID NO. 1. The BnaMYB73.C03 gene participates in the color generation process of rape petals, the gene is related to the anthocyanin content, and the stronger the expression is, the stronger the anthocyanin synthesis capability is, so that the BnaMYB73.C03 gene can be used for breeding a new variety of brassica napus. The research finds that the color of petals is deepened by over-expressing the BnaMYB73.C03 gene in the white rape. In addition, the invention also provides a corresponding transgenic breeding method, which provides a new molecular tool for rape breeding and flower color improvement, and has important ornamental value and economic benefit.

The invention discloses application of a transcription factor GhBBX21 in promoting anthocyanin accumulation in R1 cotton, and belongs to the technical field of biology. A gene CDS sequence is cloned, an overexpression vector 35S: GhBBX21-GFP is constructed, wild type arabidopsis (WT) is transformed by a pseudomonas inflorescence infection method, and an overexpression plant is obtained. Yeast single hybrid experiments prove that GhBBX21 can be combined on a promoter of an important transcriptional gene GhPAP1D for anthocyanin synthesis of R1 cotton. A dual luciferase experiment proves that the GhBBX21 can be used for remarkably promoting the expression of GhPAP1D in the R1 cotton. The results provide effective support for mining important genes related to cotton anthocyanin synthesis and analyzing a cotton anthocyanin synthesis route, and provide important support for improving cotton resistance and increasing cotton ornamental value by utilizing the genes, creating excellent cotton planting resources and cultivating insect-resistant cotton and ornamental cotton varieties.

The invention discloses an application of MaC1 and MaLc genes in breeding of anthocyanin-rich fruit bananas. According to the method, external male bud pieces (dark red), male flower petals (light red), pericarp (cyan) and pulp (white) of wild bananas are used as materials, 25 genes on the variety, content and metabolic pathway of the anthocyanin are quantitatively analyzed, and the genes relate to genes related to synthesis, modification, transport and regulation of the anthocyanin. The key gene causing anthocyanin accumulation in bananas is found out by analyzing the anthocyanin metabolism difference at the four material taking parts, so that a basis is provided for subsequently creating new germplasm rich in anthocyanin. According to the method, MaC1 and MaLc genes of bananas are further started through a MaChIII promoter, whether the MaC1 and MaLc genes can change the color of banana fruits or not is verified through transient expression, new banana germplasm with the fruits rich in anthocyanin is successfully created through an agrobacterium tumefaciens-mediated banana genetic transformation system, the anthocyanin content of the banana germplasm is greatly increased compared with that of banana varieties in the market, and the method has the advantages that the method is simple and convenient to operate and has wide application prospects. The product is beneficial to human health.

The application belongs to the field of biotechnology, and particularly relates to application of a wax plant CpMADS1 gene in regulating biosynthesis of plant anthocyanins. The wax plant CpMADS1 gene belongs to the MADS-box family, and its expression is closely related to flower color formation of the wax plant, but its function in heterologous plants such as tobacco has not been revealed. In the application, the wax plant CpMADS1 gene is introduced into tobacco, and it is found that the gene significantly inhibits synthesis of anthocyanins, and is accompanied by slight down-regulation of flavonoid content. Research shows that CpMADS1 may affect downstream metabolic flux distribution by regulating expression of key enzyme genes in the anthocyanin synthesis pathway, so as to change the flower color phenotype. The application provides a new strategy for regulating plant anthocyanin synthesis by using MADS-box genes, and has important application value for flower breeding and pigment metabolism engineering.

The application provides application of MdPHY7 protein or MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of apple anthocyanin synthesis, and belongs to the technical field of plant genetic engineering. Exogenous ALA treatment can induce MdPHY7 gene expression, thereby promoting anthocyanin synthesis increase, finally causing apple anthocyanin accumulation and promoting fruit coloring. It has been verified that overexpression of MdPHY7 gene can promote apple anthocyanin accumulation, while interference with MdPHY7 gene expression can inhibit anthocyanin accumulation and further inhibit fruit appearance quality improvement. It can be seen that MdPHY7 is a new key gene for regulating apple anthocyanin accumulation, and plays a key role in ALA regulation of apple anthocyanin accumulation, which has far-reaching significance for agricultural and forestry production, and lays a solid theoretical foundation for application of ALA in the field of agricultural production.

The invention discloses recombinant escherichia coli with high yield of cyanidin-3-O-glucoside as well as a construction method and application of the recombinant escherichia coli. The method comprises the following steps: by taking escherichia coli BL21 (DE3) engineering bacteria as a host, integrating flavanonol 4-reductase, anthocyanin synthetase and flavonoid 3-O-glucosyltransferase from different sources onto a plasmid vector pRSFDuet, and constructing to obtain a chassis cell for producing C3G; meanwhile, glutathione transferase is overexpressed, dissolution-promoting tag small ubiquitin-like modified protein is added, FaDFR is modified through enzyme engineering, FaDFR catalytic efficiency is improved through artificial protein cage space combination pathway enzyme, key precursor UDP-glucose is synthesized in an in-situ intensified mode and cofactor engineering, the cell factory for efficiently synthesizing C3G is constructed based on the combination strategy, the yield of C3G is increased, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. The titer reaches 797.20 mg / L. The method provided by the invention has the characteristics of simplicity, economy and eco-friendliness.