The invention discloses an application of MaC1 and MaLc genes in breeding of
anthocyanin-rich fruit bananas. According to the method, external male
bud pieces (dark red), male flower petals (light red), pericarp (cyan) and pulp (white) of wild bananas are used as materials, 25 genes on the variety, content and
metabolic pathway of the
anthocyanin are quantitatively analyzed, and the genes relate to genes related to synthesis, modification, transport and regulation of the
anthocyanin. The key
gene causing anthocyanin accumulation in bananas is found out by analyzing the anthocyanin
metabolism difference at the four material taking parts, so that a basis is provided for subsequently creating new
germplasm rich in anthocyanin. According to the method, MaC1 and MaLc genes of bananas are further started through a MaChIII
promoter, whether the MaC1 and MaLc genes can change the color of banana fruits or not is verified through transient expression, new banana
germplasm with the fruits rich in anthocyanin is successfully created through an
agrobacterium tumefaciens-mediated banana genetic transformation
system, the anthocyanin content of the banana
germplasm is greatly increased compared with that of banana varieties in the market, and the method has the advantages that the method is simple and convenient to operate and has wide application prospects. The product is beneficial to
human health.