Lycium barbarum gene, and coding protein, recombinant vector and application thereof

A technology of wolfberry and red wolfberry, applied in the field of plant genetic engineering, can solve problems such as complex reactions

Active Publication Date: 2021-09-03
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reaction from dihydroflavonols to anthocyanins is very complex and requires the action of several different enzymes (Johnson et al., 2001)

Method used

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  • Lycium barbarum gene, and coding protein, recombinant vector and application thereof
  • Lycium barbarum gene, and coding protein, recombinant vector and application thereof
  • Lycium barbarum gene, and coding protein, recombinant vector and application thereof

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Embodiment Construction

[0036] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. the embodiment. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0037] Aiming at the fact that the key genes related to anthocyanin synthesis in Lycium barbarum have not been found and studied so far, the inventors found that the main reason is that the current genes related to anthocyanin synthesis are obtained from the leaf transcriptome database, and the genes specifically expressed in the fruit are omitted. . For this reason, the inventors u...

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Abstract

The invention discloses a lycium barbarum gene, and a coding protein, a recombinant vector and application thereof. The amino acid sequence of the lycium barbarum gene is disclosed as SEQ ID NO. 1 or SEQ ID NO. 2. The nucleotide sequence is disclosed as SEQ ID NO. 3 or SEQ ID NO. 4. The invention also discloses a recombinant vector, expression cassette, transgenic cell line, recombinant bacterium or host cell containing the gene. The invention also discloses application of the gene in regulating anthocyanin synthesis, and a method for regulating anthocyanin synthesis in plants. The black lycium barbarum LMH1 and red lycium barbarum Ningqi7 fruit transcriptome is selected for sequencing, and the major gene for controlling the black fruit characters of the black lycium barbarum is analyzed and predicted, thereby providing foundation for researching the lycium barbarum anthocyanin synthesis mechanism. The invention also provides a direction and target for improving the anthocyanin content by modifying plants by gene engineering means.

Description

[0001] This application is a divisional application of the Chinese patent application with the application number "201810601153.X" and the invention title "Lycium barbarum gene and its encoded protein, recombinant vector, and its use". technical field [0002] The invention belongs to the field of plant genetic engineering, and relates to a gene AN2 regulating anthocyanin synthesis and metabolism, including a recombinant plasmid of the gene, a host and application thereof. Background technique [0003] Black wolfberry is a unique wild plant resource in the desert area of ​​northwest my country. In the classic Tibetan medicine "Jingzhu Materia Medica", black wolfberry is a traditional Chinese herbal medicine that has been used in China for thousands of years. It can be used to treat heart disease, irregular menstruation and menopause, etc. (J. Zheng et al., 2011) . Black wolfberry plants have special physiological characteristics of drought resistance and salt tolerance, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13Y02A50/30
Inventor 刘宝龙张怀刚宗渊朱雪冰曹东席杏媛陈文杰沈裕虎
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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