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InDel molecular marker closely linked with eggplant anthocyanin synthesis gene as well as primer and application of InDel molecular marker

A technology of molecular markers and eggplant flowers, applied in the fields of molecular genetics and molecular biology, can solve the problems of undeveloped molecular markers and positioning studies of genes

Active Publication Date: 2020-11-10
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently, the mutant p w Gene related molecular markers and localization studies have not yet been carried out

Method used

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  • InDel molecular marker closely linked with eggplant anthocyanin synthesis gene as well as primer and application of InDel molecular marker
  • InDel molecular marker closely linked with eggplant anthocyanin synthesis gene as well as primer and application of InDel molecular marker
  • InDel molecular marker closely linked with eggplant anthocyanin synthesis gene as well as primer and application of InDel molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Acquisition of Molecular Marker InDel87335789

[0031] (1) Genetic population construction and genetic analysis

[0032] 1. Test materials

[0033] Plant material: 2 eggplant purified inbred lines with green fruit color (variety number E4453) bred by the Institute of Vegetables, Guangdong Academy of Agricultural Sciences through multiple generations of strict bagging self-breeding were used as female parents (genotype p w p w YY) and the male parent (genotype is PPyy), the hybrid combination F was prepared 1 (E4453F 1 ), F 1 F 2 (E4453F 2 ). The fruit color of both female and male parents is green, and no anthocyanin synthesis can be observed in hypocotyls, veins, flowers and sepals throughout the growth period; F 1 The hypocotyls, leaf veins, flower color and fruit color of the generation materials were all purple-red; F 2 The segregation ratio of purple-red plants and green fruit-colored plants of the segregation generation is consistent with 9:7 ( f...

Embodiment 2

[0048] Example 2 Application of Molecular Marker InDel87335789

[0049] 1. Plant material: 2 eggplant purified inbred lines with green fruit color (variety number E4453) selected by the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences through multiple generations of strict bagging self-breds were used as female parents (genotype is pwpwYY) And male parent (genotype is PPyy), prepare hybrid combination F1 (E4453 F1), F1 single plant selfing to get F2 (E4453 F2) as experimental material.

[0050] 2. Genomic DNA extraction and detection

[0051] The young leaves of eggplant parent, female parent, F1 and F2 were extracted by CTAB method. The specific steps are as follows: Take 0.5g of fresh and young leaves and quickly grind them into powder in liquid nitrogen, put them in a 1.5mL centrifuge tube; add 0.5mL of extraction buffer, and put them in a water bath at 65°C; after cooling, add 0.5mL of phenol- Chloroform-isoamyl alcohol (25:24:1 volume ratio), m...

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Abstract

The invention belongs to the field of molecular biology, particularly relates to the field of molecular genetics, and particularly relates to an InDel molecular marker closely linked with an eggplantanthocyanin synthesis gene as well as a primer and application of the InDel molecular marker. For developing the molecular marker for controlling eggplant anthocyanin synthesis epistatic genes, the molecular marker InDel87335789 closely linked with the eggplant anthocyanin synthesis mutant pw gene is developed from a small associated region for restraining the eggplant anthocyanin synthesis mutantpw gene, wherein the eggplant anthocyanin synthesis mutant pw gene is positioned on the chromosome 8 of an eggplant, and the genetic distance between the molecular marker and a target gene P is 0.65cM. The molecular marker is expected to be applied to molecular marker assisted breeding of the eggplant anthocyanin synthesis mutant pw gene; and the molecular marker and the amplification primer ofthe molecular marker can be simply, conveniently and quickly applied to eggplant fruit color breeding practice with high throughput, and lay a good foundation for cloning an eggplant mutant pw gene and researching functions of the eggplant mutant pw gene.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to the field of molecular genetics, in particular to an InDel molecular marker closely linked with an eggplant anthocyanin synthesis gene and its primer and application. Background technique [0002] Anthocyanin synthesis in eggplant is a complex trait controlled by multiple genes. The applicant found eggplant fruit color genetic phenomenon controlled by 2 pairs of epistasis effect genes in eggplant breeding practice ( figure 1 ). That is, after crossing two homozygous eggplants with green fruit color, F 1 Substitute fruit color is purple, F 2 The segregation ratio of purple-red and green-colored plants was 9:7, indicating that the anthocyanin synthesis of the pericarp was controlled by two epistatic gene loci, and the two green-colored eggplants were due to the control of anthocyanin biosynthesis process Two independent and complementary gene loci (P gene and Y gene) were cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李植良任璇孙保娟李涛宫超衡周黎振兴
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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