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The application discloses a kind of CL-AuNPs@CD-MOF / Zein-PLA antibacterial films and preparation method and application thereof.The application is prepared gold nanoparticle (AuNPs) by green synthesis strategy, with CD-MOF as stabilizer, p-coumaric acid (p-CA) as natural reducing agent, by reducing tetrachloroauric acid (HAuCl4), CD-MOF is obtained by covalent crosslinking modification CL-AuNPs@CD-MOF with diphenyl carbonate (DPC), finally it is introduced into corn alcohol-soluble protein (Zein)-polylactic acid (PLA) composite film, utilize Zein excellent oxygen barrier property and PLA excellent mechanical property mutual coordination, construct the respiration microenvironment with suitable water vapor and oxygen permeability, so as to effectively inhibit the proliferation of microorganism on the surface of agaricus bisporus, and maintain its sensory quality.The composite film has significantly enhanced water stability, realizes the sustained release of gold nanoparticles, can effectively inhibit the microbial infection of agaricus bisporus after harvest.