The invention discloses a cultivation method suitable for ledum palustre in a high and cold environment. The cultivation method comprises the following cultivation steps that seed sources are screened and collected; carrying
out stress resistance treatment on the seeds; performing
quality optimization configuration; three-stage stress-resistant
domestication seedling raising; performing frozen soil isolation planting; dynamic environment monitoring; and
overwintering management. According to the application,
gibberellin physiological activation and program-controlled gradient freezing processes are adopted to induce cold-resistant
protein (LEA
protein) expression, so that the seed
germination rate is increased from 38% to 86%, an
antioxidant enzyme system in the seeds is activated by low-
temperature stress, the SOD activity is increased by 2.1 times, the
malondialdehyde content is reduced by 62%, and the
cell membrane stability is remarkably enhanced; a
temperature gradient decreasing and short-day stress
seedling raising
system is constructed,
radicle germination and
cotyledon expansion are promoted, the activity of a photosynthetic assimilation key
enzyme Rubisco is stimulated to be improved by 40%, the
chlorophyll a / b ratio is optimized to 3.2, the
proline accumulation amount reaches 180% of that of wild seedlings, the soluble
sugar content is improved by 90%, and a low-
temperature resistance physiological basis is formed.