80 results about "Propanedial" patented technology

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention provides the cassava low-temperature-resistant MeSR45-2 gene cloned from cassava for the first time, the expression of the MeSR45-2 gene in cassava is inhibited under low-temperature stress, and research shows that the gene can significantly improve the low-temperature resistance of cassava, reduce the malondialdehyde content, proline cumulant and the like in cassava, reduce leaf damage under low-temperature treatment and improve the yield of cassava. Terminal buds under low-temperature treatment are not affected and can normally grow, and expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of plant low temperature resistance and the like, and also provides a new gene resource for cultivation of a new variety of low temperature resistant cassava and construction of a new variety of transgenic cassava.

The invention provides application of an OsERF8 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsERF8 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsERF8 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of an oserf8 mutant plant under PEG induction and soil dehydration drought stress is remarkably improved; and the survival rate of the overexpressed plant OsERF8-OE under the induction of PEG (Polyethylene Glycol) is obviously reduced. Compared with a wild type plant, after PEG induces drought stress, the malondialdehyde content of the overground part of the oserf8 mutant plant is reduced, and the proline content is increased; the malondialdehyde content of the overground part of the overexpressed plant OsERF8-OE is increased, and the proline content is reduced. In conclusion, the OsERF8 gene is a potential candidate new target for researching a rice drought resistance regulation mechanism.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an OsNAC4 gene in regulation and control of rice salt tolerance. It is found for the first time that mutation of the OsNAC4 gene can improve the salt tolerance of rice seeds in the seedling stage. Under salt stress, the seedling survival rate of the mutant OsNAC4 strain is obviously higher than that of a wild type. Under normal treatment, the proline content, the hydrogen peroxide content and the malondialdehyde content of the wild type strain and the Osnac4 mutant strain have no significant difference, but after salt stress, the proline content of the Osnac4 mutant strain is significantly higher than that of the wild type strain, and the hydrogen peroxide content and the malondialdehyde content are both lower than those of the wild type strain. Therefore, the OsNAC4 gene can be utilized to cultivate an improved rice material suitable for saline-alkali soil planting through a biotechnology, a new thought is provided for cultivating salt-tolerant rice varieties, and a theoretical basis is also provided for increasing the yield of other crops by utilizing a heterologous gene expression technology.

The invention belongs to the field of rape breeding, and particularly discloses a sclerotinia rot resistance related gene BnaCLE20 of rape and application of the sclerotinia rot resistance related gene BnaCLE20 in rape breeding. The sequence of the BnaCLE20 gene is as shown in SEQ ID NO. 1, and the coded protein is as shown in SEQ ID NO. 2; the BnaCLE20 gene of the brassica napus is identified to be capable of regulating the sclerotinia rot resistance of the brassica napus for the first time, a molecular network for regulating the sclerotinia rot resistance of plants is enriched, and the BnaCLE20 gene plays an important role in researching a molecular mechanism formed by the disease resistance of the plants. The overexpression of the BnaCLE20 gene disclosed by the invention can reduce the content of active oxygen, malondialdehyde and oxalic acid in rape leaves infected by sclerotinia sclerotiorum; meanwhile, the activity of catalase in the rape leaves infected by sclerotinia sclerotiorum is enhanced; the sclerotiniose resistance of the oilseed rape is remarkably improved, and the method has important significance in guaranteeing the high and stable yield of the oilseed rape by improving the sclerotiniose resistance of the oilseed rape.

The application relates to a rice peroxidase gene OsPRX31 or application of a coded protein thereof in regulating drought resistance of rice, and belongs to the technical field of genetic engineering. OsPRX31 The rice peroxidase gene OsLEA3 participates in drought stress response through an ABA signal pathway, can reduce oxidative stress damage, including reducing active oxygen and malondialdehyde accumulation, improving proline content, and up-regulating drought resistance gene expression, and enhances drought resistance. OsPRX31 Experimental verification shows that rice overexpressing the gene has a higher survival rate after drought, improved physiological indexes, reduced accumulation of oxidized substances, and up-regulated drought resistance gene expression; and knocking out the gene leads to grain abortion, and overexpressing the gene leads to normal seed setting. OsPRX31 OsPRX31 The application can effectively improve the drought resistance of rice and maintain fertility, and provides gene resources and theoretical basis for drought resistance molecular breeding.

The invention discloses a pharmaceutical composition and application thereof in preparation of a medicine for treating sudden deafness, pharmacodynamic data shows that all components of the composition have a synergistic effect, the solubility and bioavailability of the composition in water can be remarkably improved, and the pharmaceutical composition can be used for treating sudden deafness. Through an oral administration mode, the hearing threshold of cochlear ischemia reperfusion model animals can be remarkably reduced, release of inflammatory factors such as serum IL-1beta and TNF-alpha is inhibited, oxidative stress is relieved (the malondialdehyde level is reduced), so that multi-target synergistic treatment of sudden deafness is realized, the effect is remarkable, the defects that an existing drug is single in target and inconvenient to administrate are overcome, and the application prospect is wide. The important clinical application value is realized.

The invention provides an application of a BnaXTH22 gene or an encoded protein thereof in regulating aluminum toxicity resistance of plants, and belongs to the technical field of gene engineering. The invention provides an application of a BnaXTH22 gene, a protein coded by the BnaXTH22 gene or a derivative of the BnaXTH22 gene in at least one of the following aspects: adjusting the aluminum toxicity resistance and / or oxidation resistance of a plant, and culturing a plant variety with the aluminum toxicity resistance and / or oxidation resistance. The result of the embodiment of the invention shows that in an aluminum stress environment, compared with a wild type control WT, the root activity of a plant (OEs) over-expressing BnaXTH22 is improved, the malondialdehyde accumulation amount and the relative conductivity are reduced, and stronger aluminum toxicity tolerance and oxidation resistance are shown. Therefore, the BnaXTH22 gene or the encoded protein thereof can improve the aluminum toxicity resistance and oxidation resistance of the plant.

The invention discloses an anti-drunkenness composition and application thereof. The invention provides an application of a composition in preparation of an anti-drunkenness product, the composition contains edible plasma powder, xylitol, fructo-oligosaccharide, microcrystalline cellulose, mogroside and magnesium stearate, and the edible plasma powder contains immune globulin IgG. The TZK provided by the invention has obvious regulation and improvement effects on sleep behaviors, serum indexes and gastrointestinal injury after acute drinking. The oxidative stress state after long-term large-dose chronic drinking is effectively adjusted, so that the oxidative stress injury index malondialdehyde (MDA) gradually returns to the normal level; according to the present invention, the activity of the liver injury markers such as alanine transaminase (ALT) and aspartate transaminase (AST) is reduced, the liver protection effect is provided, and the gastric mucosa structure repair is promoted by increasing the gastric mucosa prostaglandin content and reducing the local MDA level;

The invention provides application of an OsATG5 gene in improvement of drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsATG5 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsATG5 gene is as shown in SEQ ID NO: 2. Compared with a wild type (WT), the survival rate of the OsATG5 gene mutant plant osatg5 under PEG-induced drought stress is obviously lower than that of the WT; the survival rate of the plant OsATG5-OE overexpressed by the OsATG5 gene under drought stress is obviously higher than that of WT. Compared with WT, the content of proline and soluble sugar in the plant OsATG5-OE overexpressed by the gene OsATG5 after drought stress induced by PEG is increased, and the accumulation amount of hydrogen peroxide and malondialdehyde is reduced; after PEG induces drought stress, the proline content and the soluble sugar content of osatg5 mutant leaves are reduced, and the hydrogen peroxide content and the malondialdehyde content are increased. In conclusion, the OsATG5 gene is a potential candidate new target for regulating and controlling the drought resistance mechanism of the rice.

The invention discloses an edible mushroom extract and application thereof as an antioxidant preparation in storage of coarse cereals. The invention provides application of an edible mushroom extract, namely application of the edible mushroom extract as an antioxidant. The invention discloses application of an edible mushroom extract in preparation of an antioxidant. The edible mushroom extract is applied to preparation of an additive for storing coarse cereals; the edible mushroom extract is used as an additive in storage of coarse cereals; the edible mushroom extract is applied to storage of coarse cereals as an additive with an anti-oxidation function. The edible mushroom extract is an agrocybe cylindracea polysaccharide extract or a pholiota adipose polyphenol extract or a grifola frondosa polyphenol extract. The edible fungus extract provided by the invention has good antioxidant activity, can inhibit oxidase systems of the coarse cereals so as to inhibit free fatty acid accumulation and / or malonaldehyde accumulation in the coarse cereals, and is free of any harmful component. The method is helpful for solving the practical problem that the coarse cereals are easy to oxidize in the storage and processing processes.

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

This invention discloses a method for extending the storage time of peaches, a composite plasma-activated water system, and a preservation system, belonging to the field of post-harvest preservation of fruits and vegetables. The method uses a quaternary gas mixture of argon, oxygen, carbon dioxide, and helium to prepare plasma-activated water through discharge. A specific ratio of tea polyphenols and chitosan is then added to obtain composite plasma-activated water. Combined with gentle pre-cooling, light-protected ultrasonic atomization spraying, and modified atmosphere buffering, this achieves a synergistic effect of antibacterial activity, water retention, antioxidant properties, and flavor preservation. This method can reduce the total bacterial count, rot rate, and weight loss rate of peaches during storage, maintain fruit firmness, enhance the activity of endogenous antioxidant enzymes, reduce malondialdehyde accumulation, and effectively retain soluble solids and titratable acids, among other flavor compounds. The process of this invention is green and mild, with no chemical residues, and can delay flesh softening and peel browning, thus extending the storage time and shelf life of peaches.

This invention belongs to the field of carbon dot technology in traditional Chinese medicine, and more specifically, relates to cinnamon carbon quantum dots, composite films, their preparation methods, and applications. The cinnamon carbon quantum dots provided by this invention are prepared from cinnamon as raw material through a hydrothermal reaction at 190–210℃ for 9–11 hours, followed by centrifugation and dialysis (molecular weight cutoff 900–1100 Da). The resulting particles are spherical with a particle size of 1.5–3.2 nm and contain O-C=O, C-O, and C-C functional groups on their surface. A composite film is prepared by mixing and drying chitosan, acetic acid solution, glycerol, and the cinnamon carbon quantum dot solution. This composite film, when used for preserving honeysuckle berries, can improve the rate of good fruit, reduce weight loss, and maintain nutritional indicators such as soluble solids, titratable acid, total phenols, ascorbic acid, anthocyanins, and flavonoids. Simultaneously, it reduces malondialdehyde content, inhibits respiration intensity, and maintains fruit firmness. It extends the shelf life by 4 days at 25℃ and by 10 days at 4℃, demonstrating promising application prospects in fruit and vegetable preservation.

The application discloses Streptomyces olivaceus LGWZYYLX-160 and application thereof, and belongs to the technical field of biological control. The Streptomyces olivaceus LGWZYYLX-160 has double functions of immunity enhancement and disease resistance promotion on peppers. In addition, the Streptomyces olivaceus LGWZYYLX-160 has antagonistic effects on pathogenic bacteria of various crops such as peppers, potatoes, peanuts, tomatoes, eggplants, cucumbers, pines, strawberries, tobaccos and corns. Meanwhile, the strain can enhance root activity and peroxidase activity of peppers, increase proline content of the roots and reduce malondialdehyde content. In summary, the application of the Streptomyces olivaceus LGWZYYLX-160 can provide excellent strain resources for immunity enhancement of pepper roots and biological control of diseases, and provide technical support for green yield increase and quality improvement cultivation of peppers.

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

The invention discloses an edible fungus extract compound preparation for storing coarse cereals as well as a preparation method and application of the edible fungus extract compound preparation. The composition provided by the invention consists of a grifola frondosa polyphenol extract, a pholiota adipose polyphenol extract and an agrocybe cylindracea polysaccharide extract. The invention also provides application of the composition: application of the composition as an antioxidant; an application of the composition in preparation of an antioxidant; application of the composition in preparation of an additive for coarse cereal storage; application of the composition as an additive in storage of coarse cereals; the composition is applied to storage of coarse cereals as an additive with an antioxidant function. The composition provided by the invention has good antioxidant activity and can be used as an antioxidant. The edible fungus extract provided by the invention can be used as a coarse cereal additive to inhibit a coarse cereal oxidase system, so that free fatty acid accumulation and / or malondialdehyde accumulation in coarse cereals are / is inhibited, and the edible fungus extract is free of any harmful component. The method is helpful for solving the practical problem that the coarse cereals are easy to oxidize in the storage and processing processes.

The invention relates to the field of plant biotechnology and molecular biology, in particular to application of a VvECT10 gene in regulation and control of drought resistance of grapes. A strain with overexpressed VvECT10 gene is slow in leaf water loss and light in withering degree under a drought condition, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) is remarkably reduced, and the activity of peroxidase (POD) and catalase (CAT) is remarkably improved, which indicates that the overexpression of the VvECT10 gene can enhance the drought resistance of the grape plant and relieve oxidative damage.

This invention provides a composition of agarwood and mugwort, its preparation method, and its application in hangover remedies, relating to the field of plant extraction technology. The hangover remedy prepared from the agarwood and mugwort composition obtained by the extraction method of this invention has excellent hangover-relieving and liver-protecting effects for patients with excessive drinking or alcohol poisoning, improving the discomfort symptoms caused by excessive drinking. The hangover remedy containing the agarwood and mugwort composition prepared by this invention accelerates the metabolism of ethanol in the liver by reducing malondialdehyde content and increasing the activity of alcohol dehydrogenase and glutathione, thus achieving the effects of relieving hangovers, protecting the liver, and alleviating hangovers. Simultaneously, the aroma of agarwood and mugwort also has a calming and soothing effect, relieving tension, anxiety, and other emotional states caused by drinking.

The invention discloses bacillus licheniformis GUPT108 and application thereof in improving the drought resistance of flue-cured tobacco, and belongs to the technical field of microorganisms. The bacillus licheniformis GUPT108 is obtained through screening and identification, and the preservation number of the bacillus licheniformis GUPT108 is CCTCC (China Center For Type Culture Collection) NO: M20252143. The potassium dissolving efficiency of the strain is 158.99% through potassium dissolving efficiency detection. Experiments prove that the bacillus licheniformis GUPT108 provided by the invention can improve the drought resistance of flue-cured tobaccos, and after root irrigation treatment is carried out by utilizing the bacillus licheniformis GUPT108, compared with a control group, the oxidation resistance of the flue-cured tobaccos can be remarkably improved after drought, the malondialdehyde content is reduced, the root activity is improved, and the root growth is promoted. Besides, after the flue-cured tobaccos are irrigated with the bacillus licheniformis GUPT108, the content of components such as total nitrogen, nicotine and total sugar in the flue-cured tobaccos after the flue-cured tobaccos are harvested can be obviously changed, and the chemical quality of the tobaccos is improved.

The invention discloses a microbial agent composition capable of promoting growth and yield increase of soybeans in saline soil and application of the microbial agent composition. A microbial agent is an enterobacter strain THD10 or a complex microbial agent formed by the enterobacter strain THD10. The microbial agent provided by the invention can significantly improve the plant height, fresh weight, dry weight, nodulation number and nitrogenase activity of soybeans. In saline soil, compared with treatment without inoculation of the microbial inoculum, inoculation treatment can significantly improve the soybean biomass, and the nitrogen fixation efficiency of a single soybean plant is improved; the SOD, CAT and POD contents of soybean leaves are increased, and the malondialdehyde content of the leaves is reduced; the method can improve the soybean yield and promote the improvement of soybean quality indexes, has very important significance for enlarging the soybean cultivation area and improving the per unit yield in China, and has important guidance and reference significance for cultivation of other crops in saline soil.

The invention belongs to the technical field of microbial fermentation, and discloses a solid-state fermentation postbiotic for adjusting the 5-hydroxytryptamine level and a preparation method and application of the solid-state fermentation postbiotic, and the solid-state fermentation postbiotic comprises fermented fermentation lactobacillus mucus IOB802 inactivated thallus components, metabolites and fermentation substrate active components. According to the invention, after solid state fermentation, by adjusting the content of neurotransmitters such as 5-hydroxytryptamine and then adjusting the relative expression level of neuronal cell apoptosis related genes Bcl-2 and Caspase 3 mRNA, nerve injury caused by sleep disorder is further improved. Meanwhile, after solid state fermentation, the prebiotics can improve oxidative stress injury caused by sleep disorder by reducing the content of lipid peroxide malonaldehyde and increasing the content of antioxidant enzyme.

The invention discloses application of an LecRK26 gene in regulation and control of drought resistance of tomatoes, relates to the technical field of plant genetic engineering, finds and verifies the important role of the LecRK26 gene in regulation and control of the drought resistance of the tomatoes for the first time, fills the blank of the LecRK26 gene of the tomatoes in drought resistance function research, enriches the research content of a drought resistance molecular mechanism of the tomatoes, and provides a new application of the LecRK26 gene in regulation and control of the drought resistance of the tomatoes. And a new view angle is provided for functional diversity research of the plant LecRK gene family. The LecRK26 gene provided by the invention can significantly improve the drought resistance of tomatoes, and through overexpression of the gene, the seed germination rate and survival rate of the tomatoes under drought stress can be significantly improved, the number of leaf stomata, the water loss rate and the malondialdehyde content can be significantly reduced, the adaptability of the tomatoes to the drought environment is enhanced, the drought resistance of the tomatoes is fundamentally improved, and the drought resistance of the tomatoes is improved. The problem that a traditional tomato variety is poor in drought resistance is solved.

The invention relates to the field of molecular pharmacology and biological medicine, and particularly discloses a new application of a natural flavonoid compound baicalein as a catalase direct activator and an application of the natural flavonoid compound baicalein in preparation of drugs for preventing and treating oxidative stress related diseases. The invention proves that baicalein is directly combined with catalase and the thermal stability and hydrolysis resistance of the catalase are enhanced through cell thermal migration experiment (CETSA), drug affinity reaction targeted stability technology (DARTS), molecular docking and other experiments. An enzymatic reaction experiment shows that baicalein can enhance catalase activity in a dose-dependent manner in a concentration range of 5-25 [mu] mol / L, and the efficiency of catalytic decomposition of hydrogen peroxide is improved. A free fatty acid (FFA)-induced hepatocyte lipid accumulation model is adopted for cell experiment, and the result shows that by means of baicalein treatment, the activity of catalase in cells can be enhanced, the content of lipid peroxidation product malondialdehyde (MDA) can be reduced, meanwhile, the level of triglyceride (TG) can be reduced, the effect is reversed by a catalase inhibitor aminotriazole, and the activity of the catalase in the cells can be improved. It is proved that baicalein can remove hydrogen peroxide in cells by activating catalase activity, oxidative stress injury is relieved, and then the process of the non-alcoholic fatty liver disease (NAFLD) is delayed. The invention defines that baicalein can directly activate the activity of catalase, and the baicalein has an important application prospect in preparation of drugs for treating or preventing oxidative stress related diseases such as NAFLD, type 2 diabetes mellitus and neurodegenerative diseases as a natural-source catalase activator.

The invention discloses a streptomyces sp. LGWZYY-008 and an application of the streptomyces sp. LGWZYY-008. Belongs to the technical field of biocontrol. The streptomyces LGWZYY-008 disclosed by the invention has the advantages that the bacteriostasis rate of the streptomyces LGWZYY-008 on various plant disease pathogenic bacteria is up to 50% or above, and particularly, the bacteriostasis rate on tobacco target leaf spot pathogenic bacteria is up to 79% or above; the streptomyces LGWZYY-008 has immunopotentiation and disease-resistant and growth-promoting effects on tobacco, the disease-resistant and growth-promoting effects under different nitrogen fertilizer application amounts are verified, and the streptomyces LGWZYY-008 is found to be capable of remarkably improving the yield and the quality of the tobacco under treatment of 0 kg / mu nitrogen fertilizer and 7 kg / mu nitrogen fertilizer, and the disease control effect on tobacco black shank reaches up to 75%; meanwhile, the tobacco root activity and peroxidase activity can be remarkably improved, and the malondialdehyde activity of the root is reduced. In conclusion, the application of the streptomyces LGWZYY-008 can provide a high-quality biocontrol strain for tobacco root system immune enhancement and disease biological control, and lays a foundation for tobacco yield increase, quality improvement and green production.

This invention discloses a sugarcane salt tolerance gene. ShPP2C.F2-1 Its encoded proteins and applications, sugarcane salt tolerance genes ShPP2C.F2‑ 1 The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO:2. This gene enhances plant salt tolerance through a dual pathway of peroxidase-led antioxidant system and ion homeostasis regulation. Overexpression of this gene in rice significantly increased peroxidase activity, significantly decreased reactive oxygen species and malondialdehyde (MDA) content, while reducing sodium ion accumulation and stabilizing potassium ion levels, ultimately improving the survival rate of rice seedlings under salt stress. This invention clarifies... ShPP2C.F2-1 The salt tolerance function and core regulatory mechanism of genes provide new gene resources and key technical support for the genetic improvement of salt tolerance in crops such as sugarcane and rice, and have important application value for breeding new salt-tolerant crop varieties.

The invention discloses a method for evaluating the toxicity of a lithium ion battery cathode material lithium cobalt oxide to plants. The method comprises the following steps: preparing LCO with a nanosheet layer structure; simulating a plant root microenvironment, and analyzing physicochemical characteristics of LCO in the plant root microenvironment; the LCO is exposed in a target plant growth system, and the environmental toxicity of the LCO to the plant is comprehensively judged by detecting phenotypic indexes, photosynthesis parameters, root ultrastructure integrity, active oxygen distribution, malondialdehyde content and superoxide dismutase activity of the plant. The method for evaluating the toxicity of the lithium cobalt oxide serving as the positive electrode material of the lithium ion battery to the plants has important significance on revealing the ecological risk of the positive electrode material of the battery and establishing a standardized detection technology system.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The application belongs to the technical field of biotechnology, and particularly discloses a method for enhancing drought resistance of eggplants by using bacillus complex microbial inoculant and arbuscular mycorrhizal fungi, and the method forms a synergistic microbial combination by applying the bacillus complex microbial liquid and the arbuscular mycorrhizal fungi inoculant to the eggplant seedling rhizosphere. Experiments show that the combination can significantly improve the root system configuration of eggplants under drought stress, increase the root length, fresh weight and dry weight, improve the leaf relative water content and photosynthetic efficiency, reduce the malondialdehyde content of membrane lipid peroxidation products, improve the peroxidase and catalase activities, synergistically promote the absorption of nitrogen elements by the plants, and improve the rhizosphere soil enzyme activity. The method is simple in operation and stable in effect, and provides a new way for developing efficient and environmentally-friendly microbial fertilizer and improving the drought resistance of solanaceous crops.