117 results about "Propanedial" patented technology

The invention relates to an application of Danjie emodin in preparation of drugs for preventing or treating drug-induced liver injury. The liver injury is drug-induced liver injury induced by acetaminophen. According to the invention, the effective component Danleaf emodin in the traditional Chinese medicine rhubarb is used for preventing and treating drug-induced liver injury induced by acetamido, and the traditional Chinese medicine composition has the characteristics of safety, reliability and small toxic and side effects. Starting from the whole, multi-target intervention is performed on drug-induced liver injury induced by acetaminophen, the levels of glutamic-pyruvic transaminase, glutamic oxalacetic transaminase and malondialdehyde in plasma are remarkably reduced, the obvious anti-inflammatory and liver cell apoptosis inhibiting effects are achieved, the good clinical application prospect is achieved, and a new way is provided for prevention and treatment of liver injury.

The invention belongs to the technical field of microorganisms, and particularly relates to application of Ackerman muciniphila in preparation of diabetic metabolic disease related renal injury products. The preservation number of the ackermann muciniphile is CGMCC (China General Microbiological Culture Collection Center) No.20955. The ackermann muciniphile provided by the invention can be used for remarkably reducing the indexes of blood urea nitrogen, serum creatinine and urine protein and improving the renal function; the oxidative stress of the kidney is relieved by reducing the malondialdehyde level and improving the activity of SOD, CAT and GSH-Px; the TLR4 / NF-kappa B pathway is inhibited, TNF-alpha and IL-1beta secretion is reduced, and inflammatory response is relieved; finally, the kidney protection effect is achieved through the anti-oxidation, anti-inflammatory and anti-fibrosis effects.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention discloses an endophytic fungus, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.41218. The endophytic fungus has the advantages that the endophytic fungus can be used for preparing a culture medium; the endophytic fungus strain PG-2 can increase the antioxidant enzyme activity of monocotyledons (high wheatgrass and triticale) and dicotyledons (green vegetables and calcium vegetables), promote the accumulation of permeability regulating substances and reduce the content of malondialdehyde in the body. The PG-2 is a drought-resistant strain which has oxidation resistance and drought resistance and can promote plant growth, and has huge development potential in agricultural production.

The invention discloses a high-yield protease salt denitration mycobacillus for improving the quality of low-salt shrimp paste, and belongs to the field of food biotechnology and application. The salt denitration mycobacillus XJ-229 provided by the invention has relatively good salt tolerance (the tolerance range is 1%-22%) and enzyme production (the protease production is 15.38 U / mL) capacity, and a proper fermentation agent is provided for fermentation of low-salt shrimp paste. The cladobacillus sp. XJ-229 is used for preparing shrimp paste, the content of volatile basic nitrogen, malondialdehyde and biogenic amine can be reduced, the content of free amino acid can be increased, the flavor of the shrimp paste can be improved, and the prepared shrimp paste can be accepted by people more easily. Therefore, the salt denitration mycobacillus XJ-229 provided by the invention is beneficial to improving the quality of the shrimp paste and improving the market acceptability of the shrimp paste.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention provides a cordyceps militaris fermentation extract and application thereof in preservation of agaricus bisporus, and belongs to the technical field of deep processing of edible mushrooms and preservation of agricultural products. The preparation method of the cordyceps militaris fermentation extract comprises the following steps: (1) taking cordyceps militaris sporocarp, adding water, grinding, and performing enzymolysis by using cellulase and papain in sequence to obtain cordyceps militaris pulp; (2) adding glucose and waste beer yeast powder into the cordyceps militaris pulp, then inoculating lactobacillus paracasei and bifidobacterium bifidum, and fermenting; and (3) after fermentation is completed, carrying out ultrasonic crushing, centrifuging to remove thalli, and carrying out spray drying to obtain the cordyceps militaris fermentation extract. The method can effectively reduce the browning index and weight loss ratio of agaricus bisporus, can slow down the increase of malonaldehyde, reduces the activity of PPO enzyme, and prevents enzymatic browning caused by the synergistic effect of the PPO enzyme and peroxidase. The SOD enzyme activity in the agaricus bisporus can be kept, active oxygen in tissues can be removed, and the metabolic balance of the active oxygen can be maintained.

The invention relates to the technical field of crop planting, and provides a method for promoting germination of brassica napus seeds under low-temperature stress by using allantoin. The method comprises the following steps: firstly, disinfecting cabbage type rape seeds, and then soaking the cabbage type rape seeds in an allantoin solution with a specific concentration under room-temperature and weak-light conditions; the treated seeds do not need to be washed and are directly placed in a culture dish paved with double-layer filter paper, a proper amount of distilled water is added, and then the seeds are cultured in an incubator simulating a low-temperature environment. Experimental results show that the germination rate, the germination index and the vitality index of the brassica napus seeds are remarkably improved through allantoin treatment, and the average germination time is shortened. Mechanism research finds that allantoin improves the low-temperature resistance of the brassica napus by various ways of increasing the content of free water of seedlings, increasing the content of soluble sugar and proline, enhancing the activity of antioxidant enzyme, reducing active oxygen and malonaldehyde accumulation and the like. The method disclosed by the invention is low in cost and environment-friendly, and provides powerful guarantee for field production such as late direct seeding of the brassica napus.

The invention provides the cassava low-temperature-resistant MeSR45-2 gene cloned from cassava for the first time, the expression of the MeSR45-2 gene in cassava is inhibited under low-temperature stress, and research shows that the gene can significantly improve the low-temperature resistance of cassava, reduce the malondialdehyde content, proline cumulant and the like in cassava, reduce leaf damage under low-temperature treatment and improve the yield of cassava. Terminal buds under low-temperature treatment are not affected and can normally grow, and expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of plant low temperature resistance and the like, and also provides a new gene resource for cultivation of a new variety of low temperature resistant cassava and construction of a new variety of transgenic cassava.

The invention provides application of an OsERF8 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsERF8 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsERF8 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of an oserf8 mutant plant under PEG induction and soil dehydration drought stress is remarkably improved; and the survival rate of the overexpressed plant OsERF8-OE under the induction of PEG (Polyethylene Glycol) is obviously reduced. Compared with a wild type plant, after PEG induces drought stress, the malondialdehyde content of the overground part of the oserf8 mutant plant is reduced, and the proline content is increased; the malondialdehyde content of the overground part of the overexpressed plant OsERF8-OE is increased, and the proline content is reduced. In conclusion, the OsERF8 gene is a potential candidate new target for researching a rice drought resistance regulation mechanism.

The invention discloses bacillus velezensis with saline-alkaline tolerance and growth promotion functions, a bio-organic fertilizer and application of the bio-organic fertilizer, and relates to the technical field of agricultural microorganisms. The bacillus velezensis is preserved in the Guangdong Microbial Culture Collection Center on November 6, 2024, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC (China General Microbiological Culture Collection Center): 65428. The strain has strong saline-alkaline tolerance, can effectively survive in moderate and severe saline-alkaline environments, remarkably improves the germination rate of crops and the content of soluble sugar, proline and antioxidant enzyme, and reduces the content of malondialdehyde. The bio-organic fertilizer produced by using the strain can effectively improve crop yield and soil organic carbon, reduce pH and total salt content, and has a remarkable improvement effect on saline-alkali soil.

The invention relates to the technical field of healthy food, and discloses a healthy food composition for protecting liver and relieving fatigue and a preparation method of the healthy food composition for protecting liver and relieving fatigue. 5 to 10 parts of propolis; 5-7 parts of a chrysanthemum extract; 2-4 parts of a wolfberry extract; 7-10 parts of a cassia seed extract; 2-5 parts of a ginseng extract; 1.3 to 1.7 parts of a saffron crocus extract; the royal jelly and the propolis are combined according to the formula proportion, the prepared composition finished product has the effect of effectively relieving physical fatigue under the specific dosage, metabolite after exercise can be removed, the composition has the effect of increasing glycogen reserve, and the composition can be used for treating alcoholic liver injury model mice, and has the advantages that the composition finished product can be used for treating alcoholic liver injury; the composition can reduce the content of malondialdehyde and triglyceride in liver tissue and increase the content of glutathione, so that the composition provided by the invention not only has a liver protection effect, but also can relieve physical fatigue, reduce generation of serum urea and lactic acid after exercise and increase in-vivo glycogen reserve.

The invention discloses a production method for reducing hazardous substances of coarse cereal walnut cakes, and belongs to the technical field of food processing. The method for reducing the harmful substances in the coarse cereal walnut cake comprises the following steps: mixing lard oil, ghee and palm oil, and performing whipping; then adding egg liquid and xanthan gum, and continuing to whip; adding maltitol, baking soda and smelly powder, and then whipping; then adding the coarse cereal powder, the peanut powder and the soybean protein isolate, and uniformly stirring and mixing; and then dividing the dough into small dough to prepare walnut cakes, and putting the walnut cakes in a blast furnace at 100-140 DEG C for 8-10 minutes and then at 180 DEG C for 2-5 minutes to obtain the walnut cakes. According to the method, the quality and flavor of the coarse cereal walnut cake cannot be affected, the content of harmful substances such as 5-HMF, acrylamide and malondialdehyde in the coarse cereal walnut cake can be effectively reduced, and the method is simple in process step and suitable for industrial popularization.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an OsNAC4 gene in regulation and control of rice salt tolerance. It is found for the first time that mutation of the OsNAC4 gene can improve the salt tolerance of rice seeds in the seedling stage. Under salt stress, the seedling survival rate of the mutant OsNAC4 strain is obviously higher than that of a wild type. Under normal treatment, the proline content, the hydrogen peroxide content and the malondialdehyde content of the wild type strain and the Osnac4 mutant strain have no significant difference, but after salt stress, the proline content of the Osnac4 mutant strain is significantly higher than that of the wild type strain, and the hydrogen peroxide content and the malondialdehyde content are both lower than those of the wild type strain. Therefore, the OsNAC4 gene can be utilized to cultivate an improved rice material suitable for saline-alkali soil planting through a biotechnology, a new thought is provided for cultivating salt-tolerant rice varieties, and a theoretical basis is also provided for increasing the yield of other crops by utilizing a heterologous gene expression technology.

According to the method for prolonging the fresh-keeping period of the fruits by using the controlled atmosphere fresh-keeping box, the controlled atmosphere fresh-keeping box is prepared by improving the sealing performance of a common fresh-keeping box and covering a certain area of the common fresh-keeping box with a film, and the fruits are put into the box to be stored. When the area of the covering film is 20%, the lotus seeds are stored in a box, and 1-methylcyclopropene can be added; when the area of the covering film is 34.8%, the longan is placed in a box to be stored. Compared with the control, the modified atmosphere preservation box treatment can reduce the weight loss rate and browning degree of the lotus seeds and maintain the contents of Vc, amylose, total phenols and soluble solids; compared with a control group, the modified atmosphere preservation box treatment can inhibit browning of longan peel, delay reduction of soluble solids, acidity and vitamin C, inhibit increase of malondialdehyde content, reduce peroxidase activity and improve catalase activity and glutathione content. According to the technology, the shelf life of the fruits can be effectively prolonged, and the problems that an existing fresh-keeping method is tedious and short in fresh-keeping period are solved.

The invention discloses a seed treatment method for improving seed germination and planting efficiency of medicago sativa under drought conditions, and belongs to the field of seed treatment. According to the method for promoting seed germination, novel carbon nano quantum dots are used as an initiator to treat medicago sativa seeds; after the alfalfa seeds are subjected to initiation treatment, the drought resistance of the alfalfa seeds can be remarkably improved, seed germination and seedling growth can be promoted, the germination rate can be increased by 23%, the germination potential can be increased by 34%, the plant height can be increased by 90%, the biomass can be increased by 70%, meanwhile, the antioxidant enzyme activity can be remarkably improved by 33.1%, and the malondialdehyde content can be remarkably reduced by 26.43%. By applying the seed priming method, the planting efficiency can be improved, and meanwhile, the planting cost is reduced.

The invention belongs to the field of rape breeding, and particularly discloses a sclerotinia rot resistance related gene BnaCLE20 of rape and application of the sclerotinia rot resistance related gene BnaCLE20 in rape breeding. The sequence of the BnaCLE20 gene is as shown in SEQ ID NO. 1, and the coded protein is as shown in SEQ ID NO. 2; the BnaCLE20 gene of the brassica napus is identified to be capable of regulating the sclerotinia rot resistance of the brassica napus for the first time, a molecular network for regulating the sclerotinia rot resistance of plants is enriched, and the BnaCLE20 gene plays an important role in researching a molecular mechanism formed by the disease resistance of the plants. The overexpression of the BnaCLE20 gene disclosed by the invention can reduce the content of active oxygen, malondialdehyde and oxalic acid in rape leaves infected by sclerotinia sclerotiorum; meanwhile, the activity of catalase in the rape leaves infected by sclerotinia sclerotiorum is enhanced; the sclerotiniose resistance of the oilseed rape is remarkably improved, and the method has important significance in guaranteeing the high and stable yield of the oilseed rape by improving the sclerotiniose resistance of the oilseed rape.

The invention discloses an application of a transcription factor gene MabHLH770 of Musa communis in improvement of drought tolerance of plants. The gene MabHLH770 is an alkaline helix-loop-helix (bHLH) transcription factor which is separated and cloned from bananas balsamifera, and the nucleotide sequence of the gene MabHLH770 is as shown in SEQ ID NO. 2. Research results show that after drought stress treatment, compared with a wild type, the silent strain is higher in relative conductivity, malondialdehyde content and hydrogen peroxide content, and lower in proline content and peroxidase activity. The transcription factor MabHLH770 can effectively improve the drought tolerance of the plant. The discovery of the gene provides a new gene resource for plant stress-resistant molecular breeding, provides reference for plant cultivation in arid regions, and is beneficial to improving the utilization rate of the arid regions.

The application relates to a rice peroxidase gene OsPRX31 or application of a coded protein thereof in regulating drought resistance of rice, and belongs to the technical field of genetic engineering. OsPRX31 The rice peroxidase gene OsLEA3 participates in drought stress response through an ABA signal pathway, can reduce oxidative stress damage, including reducing active oxygen and malondialdehyde accumulation, improving proline content, and up-regulating drought resistance gene expression, and enhances drought resistance. OsPRX31 Experimental verification shows that rice overexpressing the gene has a higher survival rate after drought, improved physiological indexes, reduced accumulation of oxidized substances, and up-regulated drought resistance gene expression; and knocking out the gene leads to grain abortion, and overexpressing the gene leads to normal seed setting. OsPRX31 OsPRX31 The application can effectively improve the drought resistance of rice and maintain fertility, and provides gene resources and theoretical basis for drought resistance molecular breeding.

The invention discloses a pharmaceutical composition and application thereof in preparation of a medicine for treating sudden deafness, pharmacodynamic data shows that all components of the composition have a synergistic effect, the solubility and bioavailability of the composition in water can be remarkably improved, and the pharmaceutical composition can be used for treating sudden deafness. Through an oral administration mode, the hearing threshold of cochlear ischemia reperfusion model animals can be remarkably reduced, release of inflammatory factors such as serum IL-1beta and TNF-alpha is inhibited, oxidative stress is relieved (the malondialdehyde level is reduced), so that multi-target synergistic treatment of sudden deafness is realized, the effect is remarkable, the defects that an existing drug is single in target and inconvenient to administrate are overcome, and the application prospect is wide. The important clinical application value is realized.

The invention provides an application of a BnaXTH22 gene or an encoded protein thereof in regulating aluminum toxicity resistance of plants, and belongs to the technical field of gene engineering. The invention provides an application of a BnaXTH22 gene, a protein coded by the BnaXTH22 gene or a derivative of the BnaXTH22 gene in at least one of the following aspects: adjusting the aluminum toxicity resistance and / or oxidation resistance of a plant, and culturing a plant variety with the aluminum toxicity resistance and / or oxidation resistance. The result of the embodiment of the invention shows that in an aluminum stress environment, compared with a wild type control WT, the root activity of a plant (OEs) over-expressing BnaXTH22 is improved, the malondialdehyde accumulation amount and the relative conductivity are reduced, and stronger aluminum toxicity tolerance and oxidation resistance are shown. Therefore, the BnaXTH22 gene or the encoded protein thereof can improve the aluminum toxicity resistance and oxidation resistance of the plant.

The invention discloses an anti-drunkenness composition and application thereof. The invention provides an application of a composition in preparation of an anti-drunkenness product, the composition contains edible plasma powder, xylitol, fructo-oligosaccharide, microcrystalline cellulose, mogroside and magnesium stearate, and the edible plasma powder contains immune globulin IgG. The TZK provided by the invention has obvious regulation and improvement effects on sleep behaviors, serum indexes and gastrointestinal injury after acute drinking. The oxidative stress state after long-term large-dose chronic drinking is effectively adjusted, so that the oxidative stress injury index malondialdehyde (MDA) gradually returns to the normal level; according to the present invention, the activity of the liver injury markers such as alanine transaminase (ALT) and aspartate transaminase (AST) is reduced, the liver protection effect is provided, and the gastric mucosa structure repair is promoted by increasing the gastric mucosa prostaglandin content and reducing the local MDA level;

The invention provides application of an OsATG5 gene in improvement of drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsATG5 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsATG5 gene is as shown in SEQ ID NO: 2. Compared with a wild type (WT), the survival rate of the OsATG5 gene mutant plant osatg5 under PEG-induced drought stress is obviously lower than that of the WT; the survival rate of the plant OsATG5-OE overexpressed by the OsATG5 gene under drought stress is obviously higher than that of WT. Compared with WT, the content of proline and soluble sugar in the plant OsATG5-OE overexpressed by the gene OsATG5 after drought stress induced by PEG is increased, and the accumulation amount of hydrogen peroxide and malondialdehyde is reduced; after PEG induces drought stress, the proline content and the soluble sugar content of osatg5 mutant leaves are reduced, and the hydrogen peroxide content and the malondialdehyde content are increased. In conclusion, the OsATG5 gene is a potential candidate new target for regulating and controlling the drought resistance mechanism of the rice.

The invention discloses an edible mushroom extract and application thereof as an antioxidant preparation in storage of coarse cereals. The invention provides application of an edible mushroom extract, namely application of the edible mushroom extract as an antioxidant. The invention discloses application of an edible mushroom extract in preparation of an antioxidant. The edible mushroom extract is applied to preparation of an additive for storing coarse cereals; the edible mushroom extract is used as an additive in storage of coarse cereals; the edible mushroom extract is applied to storage of coarse cereals as an additive with an anti-oxidation function. The edible mushroom extract is an agrocybe cylindracea polysaccharide extract or a pholiota adipose polyphenol extract or a grifola frondosa polyphenol extract. The edible fungus extract provided by the invention has good antioxidant activity, can inhibit oxidase systems of the coarse cereals so as to inhibit free fatty acid accumulation and / or malonaldehyde accumulation in the coarse cereals, and is free of any harmful component. The method is helpful for solving the practical problem that the coarse cereals are easy to oxidize in the storage and processing processes.

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

This invention discloses a method for extending the storage time of peaches, a composite plasma-activated water system, and a preservation system, belonging to the field of post-harvest preservation of fruits and vegetables. The method uses a quaternary gas mixture of argon, oxygen, carbon dioxide, and helium to prepare plasma-activated water through discharge. A specific ratio of tea polyphenols and chitosan is then added to obtain composite plasma-activated water. Combined with gentle pre-cooling, light-protected ultrasonic atomization spraying, and modified atmosphere buffering, this achieves a synergistic effect of antibacterial activity, water retention, antioxidant properties, and flavor preservation. This method can reduce the total bacterial count, rot rate, and weight loss rate of peaches during storage, maintain fruit firmness, enhance the activity of endogenous antioxidant enzymes, reduce malondialdehyde accumulation, and effectively retain soluble solids and titratable acids, among other flavor compounds. The process of this invention is green and mild, with no chemical residues, and can delay flesh softening and peel browning, thus extending the storage time and shelf life of peaches.

This invention belongs to the field of carbon dot technology in traditional Chinese medicine, and more specifically, relates to cinnamon carbon quantum dots, composite films, their preparation methods, and applications. The cinnamon carbon quantum dots provided by this invention are prepared from cinnamon as raw material through a hydrothermal reaction at 190–210℃ for 9–11 hours, followed by centrifugation and dialysis (molecular weight cutoff 900–1100 Da). The resulting particles are spherical with a particle size of 1.5–3.2 nm and contain O-C=O, C-O, and C-C functional groups on their surface. A composite film is prepared by mixing and drying chitosan, acetic acid solution, glycerol, and the cinnamon carbon quantum dot solution. This composite film, when used for preserving honeysuckle berries, can improve the rate of good fruit, reduce weight loss, and maintain nutritional indicators such as soluble solids, titratable acid, total phenols, ascorbic acid, anthocyanins, and flavonoids. Simultaneously, it reduces malondialdehyde content, inhibits respiration intensity, and maintains fruit firmness. It extends the shelf life by 4 days at 25℃ and by 10 days at 4℃, demonstrating promising application prospects in fruit and vegetable preservation.

The invention relates to the technical field of gene engineering, in particular to a trifoliate orange CwPHL7 gene, a cloning method, an expression vector and application. According to the invention, a CwPHL7 gene high-expression arabidopsis thaliana transgenic plant is successfully obtained through cloning of the trifoliate CwPHL7 gene and construction and transformation of an over-expression vector, and drought stress treatment experiments find that the chlorophyll content, malondialdehyde (MDA) content, active oxygen accumulation and other key physiological indexes of the transgenic plant under drought conditions are all significantly superior to those of a non-transgenic plant, so that the CwPHL7 gene has a good application prospect. The Poncirus trifoliate CwPHL7 gene can significantly enhance the drought tolerance of plants, and is a potential drought-resistant breeding gene resource. The invention not only provides new experimental data for plant stress resistance gene function research, but also provides efficient technical means and gene resources for crop drought resistance genetic improvement, and has important theoretical significance and application value.

The invention discloses the application of L. pleurotus polysaccharide in the preparation of a drug for improving alcoholic liver damage, and relates to the field of biomedicine technology. The invention finds that L. pleurotus polysaccharide can effectively protect the liver cell structure, inhibit liver swelling caused by alcohol, and reduce the liver index; downregulate the activity of liver cell damage-related biological enzymes aspartate aminotransferase and alanine aminotransferase in serum; improve the activity of liver antioxidant enzymes, inhibit the generation of hepatocyte oxidation product malondialdehyde, and reduce the oxidative stress damage of the liver of alcoholic liver mice; at the same time, L. pleurotus polysaccharide can also reduce the levels of triglycerides and fatty acids in the liver and serum, effectively improve lipid metabolism disorders, and reduce lipid accumulation; in addition, L. pleurotus polysaccharide can reduce the release of inflammatory factors such as interleukin 6, interleukin 1β and tumor necrosis factor α, promote the body's inflammation level to restore the balance state, reduce liver inflammation, and thus improve the damage of alcohol to liver tissue.

The application discloses Streptomyces olivaceus LGWZYYLX-160 and application thereof, and belongs to the technical field of biological control. The Streptomyces olivaceus LGWZYYLX-160 has double functions of immunity enhancement and disease resistance promotion on peppers. In addition, the Streptomyces olivaceus LGWZYYLX-160 has antagonistic effects on pathogenic bacteria of various crops such as peppers, potatoes, peanuts, tomatoes, eggplants, cucumbers, pines, strawberries, tobaccos and corns. Meanwhile, the strain can enhance root activity and peroxidase activity of peppers, increase proline content of the roots and reduce malondialdehyde content. In summary, the application of the Streptomyces olivaceus LGWZYYLX-160 can provide excellent strain resources for immunity enhancement of pepper roots and biological control of diseases, and provide technical support for green yield increase and quality improvement cultivation of peppers.