RNA for improving low-temperature tolerance of plants and application thereof

Abstract

The invention provides a CRR2 gene for improving low-temperature tolerance of plants. The nucleotide sequence of the CRR2 gene is shown as SEQ ID NO:1. The invention also provides RNA (Ribonucleic Acid) correspondingly transcribed by the CRR2 gene and application of the RNA. The CRR2 gene and the corresponding transcribed RNA of the CRR2 gene can specifically respond to low-temperature stress response. The subcellular localization analysis shows that the fluorescent probe is localized in cell nucleus and cytoplasm. The CRR2 gene is further transferred into cassava, a cassava CRR2 high-expression strain is obtained, it is found that the CRR2 high-expression strain is more tolerant to low-temperature stress, the survival rate is greatly increased, meanwhile, the proline content in the CRR2 high-expression strain is remarkably increased, the malondialdehyde (MDA) content after low-temperature treatment is remarkably reduced, it is indicated that the CRR2 and the CRR2 gene can improve the low-temperature tolerance of plants, and in the future, the plant is subjected to genetic improvement by using the gene, and an effective resource can be provided for improving the low-temperature tolerance of the plant.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an RNA for improving low temperature tolerance of plants and an application thereof. Background technique [0002] Cassava (Manihot esculenta) is an important food and energy crop in the world. Its storage root is rich in starch, and it is the main source of food for nearly 800 million people in tropical and subtropical regions of the world. Our country is mainly alcohol and feed raw materials, and to meet the diversified needs of national food, the self-sufficiency rate is only 20%. Cultivated cassava has extraordinary light, heat and water utilization efficiency in the long-term evolution and artificial selection process, and its biomass production per unit area is higher than that of almost all other cultivated crops. features. However, since most of the world's hot spots are developing countries, the level of scientific and technological investment and research on c...

AI Technical Summary

Problems solved by technology

[0005]Due to the shortage of cassava cultivars resistant to low temperature, coupled with the limitations of many factors such as long breeding cycle, hybrid incompatibility, and failure to meet the flowering period in the traditional hybrid breeding method, resulting in At present, the cultivation of low temperature resistant cassava varieties lags behind

In the past, transcriptome analysis and transgenic technology have been used to obtain a large number of protein-coding genes that respond to low temperature stress and have regulatory functions under low temperature stress, but the high / low expression of these genes often affects the yield and quality of cassava

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