A kind of lncrna and its application

Abstract

The present invention provides a gene corresponding to LncRNA, the nucleotide sequence of which is shown in SEQ ID NO:1. The invention also provides the lncRNA transcribed corresponding to the gene and its application. The gene corresponding to the LncRNA of the present invention and the LncRNA transcribed thereof can specifically respond to low temperature stress response and drought stress response. The gene was further transferred to cassava, and it was found that the plants of the transgenic lines were short and narrow, and they were more tolerant to low temperature stress and drought stress, and the survival rate was also greatly improved. The content of proline in the transgenic lines was significantly increased. The content of malondialdehyde was significantly reduced after cold stress treatment, indicating that the LncRNA and its genes can regulate plant growth and improve the low temperature and drought tolerance of plants. In the future, the lncRNA and its gene can be used to genetically improve plants, providing effective resources for promoting plant growth, improving plant low temperature tolerance and drought tolerance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a LncRNA and an application thereof. Background technique [0002] Cassava has the advantages of drought resistance, barren resistance, high yield and does not compete with major cash crops, so it is widely planted in more than 100 countries and regions around the world. Since the introduction of cassava in my country, cassava has been widely grown in tropical and subtropical regions of China. The per mu yield of cassava is high, and cassava root contains a lot of starch, which is one of the main raw materials for the production of starch and industrial alcohol. Although cassava has many advantages, cassava originated in the Amazon River Basin of South America and is a typical tropical crop, so the planting area is limited to the tropical and subtropical regions of my country. Cassava is extremely sensitive to low temperature, low temperature will reduce the yield of ca...

AI Technical Summary

Problems solved by technology

In the past, conventional hybrid breeding methods were used to select cassava varieties resistant to low temperature, but conventional hybrid breeding methods have many limitations: lack of cold-resistant cassava resources, long screening period, high experimental costs, low germination rate of hybrid seeds, and various reasons Caused the difficulty of cross-breeding cassava cold-resistant varieties, with the continuous development of genetic engineering, people began to use methods such as genetic engineering and molecular biology to breed cassava

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