Method for enhancing low-temperature tolerance of rice by overexpressing zinc finger protein OsCIP3

Abstract

The invention discloses a method for enhancing low-temperature tolerance of rice by overexpressing zinc finger protein OsCIP3. The invention provides application of OsCIP3 protein or related biological materials thereof in regulation and control of low-temperature tolerance of plants. The related biological materials are nucleic acid molecules capable of expressing the OsCIP3 protein or expression cassettes, recombinant vectors, recombinant bacteria or transgenic cell lines containing the nucleic acid molecules. Experiments show that after the encoding gene of the OsCIP3 protein is introduced into the middle flower 11, the low-temperature tolerance of a plant is improved; and after the expression of the OsCIP3 in the middle flower 11 is reduced, the low-temperature tolerance of the plant is obviously reduced. It is proved that the OsCIP3 protein and the encoding gene thereof can regulate and control the low-temperature tolerance of the plant and have important significance on cultivation of new plant varieties with low-temperature tolerance.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for enhancing low temperature tolerance of rice by overexpressing zinc finger protein OsCIP3. Background technique [0002] Rice (Oryza sativa L.) originated in tropical and subtropical regions, and its optimum temperature is 25–30°C. Compared with wheat, oats and other crops, it is more susceptible to low temperature stress (Zhang Q, Chen QH, Wang SL, et al.Rice and coldstress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci. Rice: 2014, 7: 24-26.). According to the temperature and various physiological mechanisms that work in different temperature ranges, low temperature stress in plants can be divided into two categories: chilling damage and freezing injury. Chilling injury refers to low temperature injury at 0–20°C. In tropical and subtropical regions, chilling injury is the most important low temperature stress. The adapta...

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Problems solved by technology

Low temperature stress during the germination stage of rice will seriously reduce the germination rate, and also lead to rotten seedlings and dead seedlings; while the temperature at the seedling stage is lower than 15°C, which will affect the growth of rice (Zhang Q, ChenQH, Wang SL, et al.Rice and cold stress :methods for its evaluation and summary of cold tolerance-related quantitative trait loci.Rice:2014,7:24-26.), after long-term exposure to low temperature stress, the leaves turn green and turn yellow, curl and wither, and yellow bars will appear in severe cases When the booting stage is lower than 17℃, it will reduce the number of spikelets, inhibit the development of spikelets, prolong the days of heading and reduce the degree of ear extraction (Zhang ZY, Li JH, Li F, et al. OsMAPK3 phosphorylates OsbHLH002 / OsICE1 and inhibits its ubiquitination to activate OsTPP1 and enhances rice chilling tolerance.Developmental Cell:2017,43(6):731–743); Sustained exposure to low temperature during the flowering period will reduce the rate of fertilization and seed setting, resulting in empty shells and grains, directly affecting the final yield

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