100 results about "Cold tolerance" patented technology

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention discloses an application of a ClBZR1L gene in enhancing the cold resistance of watermelons by promoting proline accumulation. The invention belongs to the technical field of plant genetic engineering. The CDS sequence of the ClBZR1L gene is as shown in SEQ ID NO. 1. Experiments show that when a recombinant vector for constructing the silent ClBZR1L gene is transferred into a watermelon plant, the proline content of the silent plant under cold stress treatment is remarkably reduced compared with that of a control group, and the cold resistance is reduced. A recombinant vector for constructing the over-expression ClBZR1L gene is transferred into a watermelon plant, the proline accumulation amount of the over-expression plant under cold stress treatment is remarkably increased, the cold resistance is enhanced, and a gene resource is provided for watermelon cold-resistant molecular breeding. Besides, the invention also discloses direct combination of the ClBZR1L gene and a proline synthesis rate-limiting enzyme coding gene ClP5CS1 gene promoter, and the expression of the coding gene is up-regulated, so that synthesis of proline under cold stress is promoted, and a theoretical basis is provided for in-depth study on cold-resistant gene pathways.

The application provides a peanut germination period cold tolerance related KASP molecular marker, a primer group and an application thereof, and belongs to the technical field of molecular markers.The RIL population constructed by using the cold tolerance variety Sihelianhong and the low temperature sensitive variety Jinnonghei No.3 as materials, a SNP site closely related to the germination period cold tolerance is developed at the Chr.18:2374669 site of the peanut 18th chromosome, and the site has A / C single nucleotide polymorphism.The application also designs a primer group for detecting the SNP site, and the primer group comprises a first upstream primer, a second upstream primer and a universal downstream primer.The primer group can quickly identify the peanut germination period cold tolerance, has the advantages of accuracy, rapidness, low cost, short identification period, simple operation and the like, and provides an efficient molecular detection tool for the peanut planting industry in high latitude and low temperature areas.

This invention discloses a cultivation and monitoring device for low-temperature cold-resistant growth of rice, relating to the field of rice cultivation technology. The invention includes a three-layer frame for mounting the equipment; a supplementary lighting system is installed on the upper layer of the frame; a rice cultivation trough is installed on the middle layer; and a water tank and a chiller are installed on the lower layer. The water tank and the chiller's inlet are connected via a first pipe. The rice cultivation trough is internally divided into a cold water inlet chamber, a cold water cultivation chamber, and a cold water return chamber. Water level and flow are controlled by inlet and outlet weirs. The device is also equipped with adjustable weirs to flexibly adjust the water level to meet the needs of different rice growth stages. Simultaneously, a video recording device and a temperature recording device are installed to record the entire rice growth process and monitor water temperature in real time, providing researchers with accurate experimental data. Furthermore, the device includes a room-temperature cultivation chamber as a control, which helps evaluate the low-temperature growth ability of genetically modified or hybridized rice seeds.

The invention belongs to the technical field of biology, and particularly provides application of a ZmMAPK4 gene in enhancing cold resistance of plants. The invention provides a gene ZmMAPK4 capable of regulating the cold resistance function of corn, the gene ZmMAPK4 is transformed into corn by utilizing an agrobacterium-mediated method, and the result shows that the ZmMAPK4 obviously enhances the cold resistance of the corn. The invention provides a gene resource for researching the cold resistance of plants, and has important theoretical significance and application value.

The utility model relates to rice cultivation equipment field, a kind of rice cold tolerance identification device, including the cold water pool for storing cold water, for storing normal temperature water's water reservoir, mixing pool, first water pump, second water pump and micro-irrigation belt, the input end of first water pump is connected water reservoir and cold water pool respectively through three-way pipe line, and the output end of first water pump is connected with mixing pool, and the input end of second water pump is connected with mixing pool, and the output end of second water pump is connected micro-irrigation belt through pipe line;The micro-irrigation belt is arranged in rice cold tolerance identification site, for spraying low-temperature water to the environment of rice cold tolerance identification site.The purpose of the device is to design a kind of rice cold tolerance identification device, which is easy to build, automatic control, and less water consumption.

The application relates to the technical field of molecular biology, and discloses a cold-tolerance identification method of Pennisetum based on a 65-core molecular marker set, a primer group and a gene chip and application thereof. The application collects core germplasm resources of the Pennisetum through a system, and constructs a GWAS population by using multi-environment (different altitudes) conditions, so that a wide genetic background and phenotype variation are fully covered, and a material foundation for genetic analysis of cold tolerance is laid. In the aspect of cold-tolerance phenotype identification, the application innovatively takes the chlorophyll content of leaves and the percentage of green leaves after natural low-temperature stress in autumn and winter fields as the cold-tolerance evaluation indexes, judges individuals with more than 40 excellent allelic variations as high-cold-tolerance varieties, and establishes a rapid and non-destructive phenotype screening method which can be implemented at the seedling stage.

This invention provides a cold adaptation regulatory gene RAF3 / RAF6 This invention relates to the field of genetic engineering technology and its applications. Specifically, it provides a cold adaptation regulatory gene. RAF3 / RAF6 , wherein RAF3 The genome sequence is shown in SEQ ID No. 1. RAF6 The genome sequence is shown in SEQ ID No. 2. This invention also provides the... RAF3 / RAF6 The encoded protein, and its role in reducing RAF3 / RAF6 By reducing the expression level of a protein or decreasing its activity or content, the cold tolerance and photosynthetic efficiency of plants can be improved, meaning that under cold conditions, RAF3 and / or RAF6 It reduced photosynthesis while enhancing the expression of cold-response genes, ultimately inhibiting plant growth and promoting plant adaptation to cold stress.

This invention relates to the cassava MeABF gene and its applications, belonging to the field of plant genetic engineering and breeding technology. This invention discloses a cassava MeABF gene (… MeABF3 The application of cassava in regulating stress resistance was disclosed in detail. MeABF3 The gene can respond to drought and low temperature stress, positively regulating the drought resistance and cold tolerance of cassava. Utilizing the present invention... MeABF3 Genetically bred stress-resistant cassava materials can significantly improve the survival and growth performance of cassava under drought and low temperature conditions, expand the planting range of cassava, provide a molecular basis for high and stable cassava yield, and have important application prospects and economic value.

The present application relates to the technical field of plant genetic engineering, and particularly relates to application of GhNFXL1 gene in improving salt tolerance and / or cold tolerance of cotton plants. Through various tests, it is found that silencing of the GhNFXL1 gene can reduce the salt tolerance and cold tolerance of the plants; overexpression of the GhNFXL1 gene can enhance the salt tolerance and cold tolerance of the plants, and the results show that the GhNFXL1 positively regulates the salt tolerance and cold tolerance of the cotton plants.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis PeMASR1 gene, biological material and application thereof. The PeMASR1 gene provided by the present application is a new gene discovered in the study of cold tolerance of Phyllostachys edulis leaves, the nucleotide sequence of which is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. Through gene cloning, expression and comparative analysis, the phylogenetic position of the Phyllostachys edulis PeMASR1 gene and the important role thereof in the research of plant response to abiotic stress mechanism and photosynthesis are revealed. Overexpression in Columbia-type Arabidopsis plants can make the rosette leaves of the plants early senesce; and the plants show a more stress-tolerant phenotype than wild-type Arabidopsis in response to cold stress, drought stress and salt stress and other abiotic stresses.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

The invention relates to a chimonanthus praecox calmodulin protein CpCML25 and an application thereof. The CpCML25 gene is cloned from chimonanthus praecox, the CDS region of the CpCML25 gene is 570 bp, and 189 amino acids are encoded. Under drought, salt, high temperature and low temperature stress and induction of hormones ABA and MeJA, CpCML25 is up-regulated to different degrees. And performing agrobacterium tumefaciens-mediated transformation on the overexpression vector to obtain the 35S:: CpCML25 / arabidopsis thaliana strain. Compared with a wild type, the overexpressed strain shows the phenomena that the flowering time is advanced, the plant height is lower, the rosette leaf length is smaller, the number of stem leaves and primary lateral branches is increased and the like. The overexpression CpCML25 improves the drought resistance of the arabidopsis thaliana, and reduces the salt tolerance and cold tolerance of the arabidopsis thaliana.

The application provides a method for identifying and screening cold tolerance of elephant grass and application thereof, and relates to the field of plant cultivation. The method mainly comprises the following steps: subjecting the standard elephant grass stem segments to variable temperature germination; subjecting the standard elephant grass stem segments to constant temperature low temperature stress; subjecting the stressed stem segments to recovery culture under suitable conditions; determining and calculating the low temperature germination, stress survival rate and recovery growth rate of the stem segments; and comprehensively evaluating the cold tolerance of the stem segments based on the weighted values of the three. The application overcomes the shortcomings of the prior art, can simulate the key low temperature stress and recovery process in the laboratory, and can realize rapid, reliable and batch preliminary identification and screening of the cold tolerance of a large amount of materials, so that the blind dependence on large-scale and long-period field identification is significantly reduced, the screening accuracy and breeding efficiency are effectively improved, and the breeding period is shortened.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

The invention discloses an application of an OsBGGP gene in regulating and controlling the cold tolerance of rice in a seedling stage. The invention provides an application of a substance for inhibiting the activity of a protein OsBGGP or inhibiting the expression of a nucleic acid molecule encoding the protein OsBGGP in the following A1) or A2): A1) reducing the cold resistance of plants; a2) cultivating plants with low cold resistance; according to the present invention, the gene engineering method finds that the OsBGGP regulates the rice seedling stage cold resistance, the method for regulating the rice seedling stage cold resistance is found, and the understanding on the rice cold resistance regulation is expanded.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to plant cold resistance and application of the molecular marker. On the basis of a genome version number V4, the molecular marker is located at the 379th site of the downstream of a corn chromosome 2 Zm00001d006860 gene translation start site ATG, the polymorphism is G / A, and the cold tolerance of a plant corresponding to the genotype A is remarkably superior to that of a plant corresponding to the genotype G. The molecular marker provided by the invention can be used for detecting the cold resistance of the plant, and auxiliary identification of the cold resistance of the plant can be realized by detecting the polymorphism of the molecular marker in the plant. The molecular marker provided by the invention can significantly improve the plant breeding efficiency, and has great application value and practical significance.

The invention discloses a medicago sativa calcium-dependent protein kinase CDPK33 gene and an application thereof. According to the invention, a cDNA (complementary deoxyribonucleic acid) sequence of MsCDPK33 is cloned from medicago sativa, and the MsCDPK33 gene responds to induction of cold and drought. Researches find that interference on expression of the CDPK33 gene in alfalfa can improve the growth performance, cold tolerance and drought tolerance of plants. The invention provides a method for cultivating high-quality, cold-resistant and drought-resistant alfalfa by using the CDPK33 gene, and after the gene is interfered in alfalfa, the growth performance, cold resistance and drought resistance of transgenic alfalfa are improved; meanwhile, after the gene is over-expressed in medicago sativa, the growth performance, cold resistance and drought resistance of transgenic medicago sativa are reduced.

The invention relates to application of zeaxanthine in improving cold resistance of plants, and belongs to the technical field of agriculture. The invention provides an application of zeaxanthine in improving the cold resistance of a plant. The plant is dendrobium nobile. According to the method, zeaxanthine aqueous solutions with different concentrations are sprayed to the low-temperature-stressed dendrobium nobile, and by measuring various parameters of the dendrobium nobile, results show that the yellow leaf rate, the leaf falling rate and the relative electric conductivity of plants can be reduced by spraying the zeaxanthine aqueous solution with the concentration of 50 mg / L to the low-temperature-stressed dendrobium nobile plants, the low-temperature semi-lethal temperature of the plants is reduced, and the yield of the plants is increased. The content of malonaldehyde, soluble protein, soluble sugar, proline and chlorophyll in the plants can be increased, the stress resistance of the plants is enhanced, damage caused by low temperature is relieved, and it is comprehensively known that the cold resistance of the dendrobium nobile can be enhanced by externally applying the zeaxanthine aqueous solution with the concentration of 50 mg / L.

This invention relates to the application of the maize BRI1a gene in regulating plant cold tolerance. The invention discovers that the maize BRI1a protein can negatively regulate plant cold tolerance; by reducing the expression level of the BRI1a gene, plant cold tolerance can be effectively improved, thus increasing the survival rate of plants under low-temperature environments. This invention utilizes CRISPR / Cas9 technology to obtain cold-tolerant BRI1a gene knockout homozygous lines, providing new gene resources for breeding transgenic plants with improved cold tolerance and improving cold-tolerant plant germplasm resources.

The invention relates to the technical field of fertilizers, and particularly discloses a cold-resistant compound microbial fertilizer and a preparation method thereof.The cold-resistant compound microbial fertilizer is prepared from cold-resistant microbial flora, an organic carrier, an inorganic anti-freezing synergistic component and a compound anti-freezing protective agent; according to the preparation method, the adaptability of a low-temperature environment can be remarkably improved, the retention rate of the total viable count is greater than 85% after storage at-15 DEG C for 3 months, the freezing point is reduced by 2.5-3.0 DEG C through microorganism domestication, gene modification and an anti-freezing system compounding, the growth of ice crystals is effectively inhibited, the survival rate of microorganisms at low temperature is guaranteed, and the survival rate of the microorganisms at low temperature is improved. A grid structure formed by the composite anti-freezing protective agent can buffer osmotic pressure change, and cell rupture is avoided.

The present application relates to the field of genetic engineering, in particular to the application of ZmHSP22 protein and its coding gene in regulating the low temperature stress tolerance of plants. The nucleotide sequence of ZmHSP22 gene is shown as SEQ ID No. 1; the protein amino acid sequence is shown as SEQ ID No. 3. After knocking out the ZmHSP22 gene, the cold tolerance of the transgenic plants is improved compared with the control group under low temperature stress treatment. The present application verifies that the ZmHSP22 protein has a regulating function on the low temperature stress tolerance of plants (especially corn), and the ZmHSP22 protein and its related biological materials can be applied to the regulation of the cold tolerance of plants, providing excellent candidate gene resources for the cultivation and improvement of new varieties of cold-tolerant plants. The method for breeding cold-tolerant plants provided has strong purpose compared with the traditional breeding method, significantly shortens the period of cold-tolerant breeding, improves the efficiency of cold-tolerant breeding, lays a theoretical foundation for the study of the mechanism of plant response to adverse signals and the molecular mechanism of tolerance to adverse environment, and has important theoretical and practical significance for promoting the process of corn breeding.

The invention relates to an application of a corn ZmXTH27 gene in improving the cold resistance of plants. The ZmXTH27 gene encodes a protein of a xyloglucan endotransglycosidase / hydrolase (XTH) family, the gene expression level of the ZmXTH27 gene is remarkably increased under low-temperature stress, and a mutant zmxth27 of the gene shows a low-temperature sensitive phenotype. The ZmXTH27 gene is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmXTH27 gene to cold stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn cold resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new cold-resistant corn strains.

The invention discloses application of a protein CK2 beta3 and a coding gene thereof in regulating and controlling the cold resistance of a plant in a seedling stage, and relates to the technical field of gene engineering. The amino acid sequence of the protein CK2 beta3 is as shown in SEQ ID NO.1, and the nucleotide sequence of the gene for coding the protein CK2 beta3 is as shown in SEQ ID NO.2. The protein CK2 beta3 and the coding gene thereof are related to the regulation of the cold resistance in the seedling stage of rice, and the analysis of the function of the CK2 beta3 gene has important theoretical significance for revealing the molecular mechanism of the regulation of the cold resistance in the seedling stage of plants. The problem that low temperature restricts plant production is effectively solved.

The invention relates to the field of plant genetic engineering and molecular breeding, in particular to a tomato SlPPR3 overexpression vector and application thereof. The nucleotide sequence of the SlPPR3 overexpression vector is as shown in SEQ ID NO. 1. The tomato overexpression vector SlPPR3 is used for improving the cold resistance of tomatoes and the size and weight of fruits at the low temperature, compared with an existing technology for improving the fruit yield of the tomatoes at the low temperature, an SlPPR3 overexpression tomato plant is obtained by constructing the SlPPR3 overexpression vector and infecting the tomatoes, the plant shows the phenotype of dark green leaves, and the yield of the tomatoes at the low temperature is improved. The tomato fruit yield is increased, and the cold resistance of the tomato is remarkably improved at the low temperature of the knockout plant. The method established by the invention can be used for regulating and controlling fruits of solanaceae crops, and can improve the biological yield of horticultural crops and accelerate the creation of new varieties.

The invention relates to application of a rice OsbZIP34 gene in enhancing low-temperature stress resistance in a rice seedling stage, and belongs to the technical field of plant genetic engineering. Specifically, the rice OsbZIP34 gene participating in positive regulation and control of low-temperature stress resistance is obtained through cloning, an overexpression strain and a knockout line of the rice OsbZIP34 gene are constructed, and it is found that compared with a wild plant, the overexpression plant of the rice OsbZIP34 gene shows the characteristic of higher low-temperature resistance, the survival rate is remarkably increased, and the knockout line shows the opposite phenotype. In conclusion, the invention discloses a new function of the rice OsbZIP34 gene, and provides a new target and a new resource for genetic breeding for improving the low-temperature stress resistance of rice.

Systems, compositions and methods for use of ice-structuring proteins (including antifreeze proteins and antifreeze glycoproteins) to prevent or reduce frost damage in seeds, seedlings, plants and plant products are disclosed. Compositions comprise one or more antifreeze proteins—derived from fish, insects, fungi, algae or bacteria—or modified variants thereof, and may include adjuvants, sugars, salts, oils, nanoparticles or microbial carriers. Delivery formats include foliar sprays, seed soaks or coatings, soil amendments, irrigation admixtures, nanoparticle-controlled release, and probiotic or endophytic microbes that secrete ice proteins in situ. Methods of application can lower freezing onset temperature, inhibit ice recrystallization to reduce tissue damage, and / or enhance plant cold-tolerance, depending on the chosen formulation. Representative formulations and experimental results (seed germination, seedling survival, reduced leaf freezing temperature and cryoprotection) are provided.

The invention discloses a CTB17 gene for regulating cold resistance of rice at a booting stage and application of the CTB17 gene. The cold resistance of the rice at the booting stage is regulated by regulating the expression or activity of the rice CTB17 gene or the coded protein thereof; and the amino acid sequence coded by the CTB17 gene is as shown in SEQ ID NO. 3. The invention provides the application of the CTB17 gene in regulating and controlling the cold resistance of the rice in the booting stage for the first time; the CTB17 gene plays an important role in regulating and controlling the cold resistance of the rice at the booting stage, provides a key gene resource for cultivating low-temperature-resistant rice varieties, and has a wide application prospect.

The invention belongs to agricultural biotechnology engineering, and particularly discloses a rice seedling stage cold tolerance locus qCTS4 and a molecular marker and application thereof. The molecular marker is a locus qCTS4 which is positioned at 32446985bp of a chromosome 4 and is remarkably related to the cold resistance in the seedling stage, and the cold resistance of the germplasm carrying A nucleotide allelic variation at the locus in the seedling stage is extremely remarkably higher than that of the germplasm carrying G nucleotide allelic variation. The method for identifying the cold resistance of the rice in the seedling stage is accurate and reliable through verification.