159 results about "Cold tolerance" patented technology

The invention discloses a pennisetum purpureum gene PpbHLH148L and application thereof in improving the cold resistance of plants, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. After the gene is subjected to subcellular localization and expression condition analysis after cold stress, the gene is related to low-temperature tolerance. An overexpression vector of the gene is constructed, Agrobacterium tumefaciens is transferred, then Arabidopsis thaliana infection is carried out, cold tolerance determination is carried out on overexpressed Arabidopsis thaliana, phenotypic difference and physiological index difference between an overexpressed strain after cold treatment and a wild type are observed, and it is found that the gene can significantly improve the low-temperature stress tolerance of Arabidopsis thaliana. And in the low-temperature treatment of the pennisetum alopecuroides transgenic callus, the transgenic callus also shows higher tolerance than the wild callus at low temperature. The pennisetum purpureum gene PpbHLH148L can be used for low-temperature tolerance modification of plants, and has an application prospect in creation of low-temperature-resistant plant materials.

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention relates to the technical field of potato cold resistance identification, in particular to a potato seedling stage cold resistance identification device which comprises a conveying mechanism. The device further comprises an environment simulation mechanism, an identification mechanism and a culture mechanism, and the environment simulation mechanism, the identification mechanism and the culture mechanism are all installed on the conveying mechanism. According to the potato seedling identification device, a seedling raising pot bearing potato seedlings is clamped through the conveying mechanism, the potato seedlings are conveyed into the environment simulation mechanism, different low-temperature environments are simulated through the environment simulation mechanism, the potato seedlings are subjected to low temperature, and then the potato seedlings are conveyed into the identification mechanism through the conveying mechanism; the cold resistance of the potato seedlings is identified through the identification mechanism, then the potato seedlings are conveyed into the culture mechanism through the conveying mechanism, the potato seedlings are cultured for a long time in the culture mechanism, and the growth condition of the potato seedlings subjected to low temperature is observed, so that the practicability of the equipment is improved.

The invention discloses an application of a ClBZR1L gene in enhancing the cold resistance of watermelons by promoting proline accumulation. The invention belongs to the technical field of plant genetic engineering. The CDS sequence of the ClBZR1L gene is as shown in SEQ ID NO. 1. Experiments show that when a recombinant vector for constructing the silent ClBZR1L gene is transferred into a watermelon plant, the proline content of the silent plant under cold stress treatment is remarkably reduced compared with that of a control group, and the cold resistance is reduced. A recombinant vector for constructing the over-expression ClBZR1L gene is transferred into a watermelon plant, the proline accumulation amount of the over-expression plant under cold stress treatment is remarkably increased, the cold resistance is enhanced, and a gene resource is provided for watermelon cold-resistant molecular breeding. Besides, the invention also discloses direct combination of the ClBZR1L gene and a proline synthesis rate-limiting enzyme coding gene ClP5CS1 gene promoter, and the expression of the coding gene is up-regulated, so that synthesis of proline under cold stress is promoted, and a theoretical basis is provided for in-depth study on cold-resistant gene pathways.

The invention discloses a biological breeding method for cultivating high-yield early-maturing rice with low-temperature-resistant and lodging-resistant characteristics by using a molecular recurrent assisted shuttle method. The method comprises the following steps: selecting parents which are resistant to cold, resistant to lodging and high in adaptability for multiple rounds of hybridization, screening cold-resistant and lodging-resistant strains by direct seeding in a Hainan winter (anti-season) group, carrying out stress identification in a Heilongjiang summer (in-season) cold water pool and a high-fertilizer field, and carrying out phenotype high selection pressure assisted by a genome information combined selection technology. And efficient polymerization of cold resistance, lodging resistance and high-yield and early-maturing characters is realized. According to the method, the technical bottleneck of insufficient multi-character collaborative improvement including cold resistance, disease resistance and lodging resistance in traditional early-maturing japonica rice breeding is solved, and the multi-character collaborative improvement breeding efficiency is remarkably improved. The vacant shell rate of the case variety Zhongnong japonica 5205 bred by the method is lower than 12.9% under low-temperature stress, the cold resistance is improved by more than 50% compared with that of parents, and the method has the characteristics of high yield, disease resistance and high quality.

The application provides a peanut germination period cold tolerance related KASP molecular marker, a primer group and an application thereof, and belongs to the technical field of molecular markers.The RIL population constructed by using the cold tolerance variety Sihelianhong and the low temperature sensitive variety Jinnonghei No.3 as materials, a SNP site closely related to the germination period cold tolerance is developed at the Chr.18:2374669 site of the peanut 18th chromosome, and the site has A / C single nucleotide polymorphism.The application also designs a primer group for detecting the SNP site, and the primer group comprises a first upstream primer, a second upstream primer and a universal downstream primer.The primer group can quickly identify the peanut germination period cold tolerance, has the advantages of accuracy, rapidness, low cost, short identification period, simple operation and the like, and provides an efficient molecular detection tool for the peanut planting industry in high latitude and low temperature areas.

The present invention provides a special cold-tolerant regulator for cotton seedling stage, its preparation method and application method, belonging to the technical field of crop cultivation. The special cold-tolerant regulator for cotton seedling stage includes chlorophyllin iron and chitosan; the mass ratio of chlorophyllin iron to chitosan is 28-38:500-700. The preparation method of the present invention includes the following steps: (1) Mix chlorophyllin iron with water to obtain solution A; (2) Mix chitosan with glacial acetic acid solution and dissolve to obtain solution B; (3) Add solution A to solution B, and make up the volume with water to obtain the special cold-tolerant regulator for cotton seedling stage. The application method of the present invention is to perform foliar spraying before the occurrence of chilling injury from the cotyledon stage to the five-leaf stage of cotton seedlings. Applying the cold-tolerant regulator prepared by the present invention before the occurrence of chilling injury in the cotton seedling stage can significantly reduce the chilling injury index of cotton seedling leaves and improve the survival rate of cotton seedlings.

This invention discloses a cultivation and monitoring device for low-temperature cold-resistant growth of rice, relating to the field of rice cultivation technology. The invention includes a three-layer frame for mounting the equipment; a supplementary lighting system is installed on the upper layer of the frame; a rice cultivation trough is installed on the middle layer; and a water tank and a chiller are installed on the lower layer. The water tank and the chiller's inlet are connected via a first pipe. The rice cultivation trough is internally divided into a cold water inlet chamber, a cold water cultivation chamber, and a cold water return chamber. Water level and flow are controlled by inlet and outlet weirs. The device is also equipped with adjustable weirs to flexibly adjust the water level to meet the needs of different rice growth stages. Simultaneously, a video recording device and a temperature recording device are installed to record the entire rice growth process and monitor water temperature in real time, providing researchers with accurate experimental data. Furthermore, the device includes a room-temperature cultivation chamber as a control, which helps evaluate the low-temperature growth ability of genetically modified or hybridized rice seeds.

The invention belongs to the technical field of biology, and particularly provides application of a ZmMAPK4 gene in enhancing cold resistance of plants. The invention provides a gene ZmMAPK4 capable of regulating the cold resistance function of corn, the gene ZmMAPK4 is transformed into corn by utilizing an agrobacterium-mediated method, and the result shows that the ZmMAPK4 obviously enhances the cold resistance of the corn. The invention provides a gene resource for researching the cold resistance of plants, and has important theoretical significance and application value.

The utility model relates to rice cultivation equipment field, a kind of rice cold tolerance identification device, including the cold water pool for storing cold water, for storing normal temperature water's water reservoir, mixing pool, first water pump, second water pump and micro-irrigation belt, the input end of first water pump is connected water reservoir and cold water pool respectively through three-way pipe line, and the output end of first water pump is connected with mixing pool, and the input end of second water pump is connected with mixing pool, and the output end of second water pump is connected micro-irrigation belt through pipe line;The micro-irrigation belt is arranged in rice cold tolerance identification site, for spraying low-temperature water to the environment of rice cold tolerance identification site.The purpose of the device is to design a kind of rice cold tolerance identification device, which is easy to build, automatic control, and less water consumption.

The application relates to the technical field of molecular biology, and discloses a cold-tolerance identification method of Pennisetum based on a 65-core molecular marker set, a primer group and a gene chip and application thereof. The application collects core germplasm resources of the Pennisetum through a system, and constructs a GWAS population by using multi-environment (different altitudes) conditions, so that a wide genetic background and phenotype variation are fully covered, and a material foundation for genetic analysis of cold tolerance is laid. In the aspect of cold-tolerance phenotype identification, the application innovatively takes the chlorophyll content of leaves and the percentage of green leaves after natural low-temperature stress in autumn and winter fields as the cold-tolerance evaluation indexes, judges individuals with more than 40 excellent allelic variations as high-cold-tolerance varieties, and establishes a rapid and non-destructive phenotype screening method which can be implemented at the seedling stage.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

This invention provides a cold adaptation regulatory gene RAF3 / RAF6 This invention relates to the field of genetic engineering technology and its applications. Specifically, it provides a cold adaptation regulatory gene. RAF3 / RAF6 , wherein RAF3 The genome sequence is shown in SEQ ID No. 1. RAF6 The genome sequence is shown in SEQ ID No. 2. This invention also provides the... RAF3 / RAF6 The encoded protein, and its role in reducing RAF3 / RAF6 By reducing the expression level of a protein or decreasing its activity or content, the cold tolerance and photosynthetic efficiency of plants can be improved, meaning that under cold conditions, RAF3 and / or RAF6 It reduced photosynthesis while enhancing the expression of cold-response genes, ultimately inhibiting plant growth and promoting plant adaptation to cold stress.

This invention relates to the cassava MeABF gene and its applications, belonging to the field of plant genetic engineering and breeding technology. This invention discloses a cassava MeABF gene (… MeABF3 The application of cassava in regulating stress resistance was disclosed in detail. MeABF3 The gene can respond to drought and low temperature stress, positively regulating the drought resistance and cold tolerance of cassava. Utilizing the present invention... MeABF3 Genetically bred stress-resistant cassava materials can significantly improve the survival and growth performance of cassava under drought and low temperature conditions, expand the planting range of cassava, provide a molecular basis for high and stable cassava yield, and have important application prospects and economic value.

The present application relates to the technical field of plant genetic engineering, and particularly relates to application of GhNFXL1 gene in improving salt tolerance and / or cold tolerance of cotton plants. Through various tests, it is found that silencing of the GhNFXL1 gene can reduce the salt tolerance and cold tolerance of the plants; overexpression of the GhNFXL1 gene can enhance the salt tolerance and cold tolerance of the plants, and the results show that the GhNFXL1 positively regulates the salt tolerance and cold tolerance of the cotton plants.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a cold-resistant and bolting-resistant related gene BraTZF3 of Chinese cabbage and application of the cold-resistant and bolting-resistant related gene BraTZF3. A stress-related transcription factor BraA01g008400.3 C is screened by combining a yeast stress library screening technology with transcriptome analysis, the stress-related transcription factor BraA01g008400.3 C belongs to a co-tandem C3H type zinc finger protein and comprises a C-x8-C-x5-C-x3-H zinc finger (ZNF) structural domain, and tests prove that the BraTZF3 is positively correlated to low-temperature stress response and cold resistance, and the BraA01g008400.3 C can be used for preparing the stress-related transcription factor BraA01g008400.3 C. According to the present invention, the BraTZF3 is subjected to heterologous overexpression to positively regulate the cold resistance and the bolting resistance of arabidopsis thaliana, such that the gene provides the positive regulation effect on the cold resistance and the bolting resistance of the plant, especially the Chinese cabbage, and the new tool and the new method are provided for improving the cold resistance and the bolting resistance of the crop by using the gene engineering means so as to provide the good practical application value;

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis PeMASR1 gene, biological material and application thereof. The PeMASR1 gene provided by the present application is a new gene discovered in the study of cold tolerance of Phyllostachys edulis leaves, the nucleotide sequence of which is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. Through gene cloning, expression and comparative analysis, the phylogenetic position of the Phyllostachys edulis PeMASR1 gene and the important role thereof in the research of plant response to abiotic stress mechanism and photosynthesis are revealed. Overexpression in Columbia-type Arabidopsis plants can make the rosette leaves of the plants early senesce; and the plants show a more stress-tolerant phenotype than wild-type Arabidopsis in response to cold stress, drought stress and salt stress and other abiotic stresses.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

The invention relates to a chimonanthus praecox calmodulin protein CpCML25 and an application thereof. The CpCML25 gene is cloned from chimonanthus praecox, the CDS region of the CpCML25 gene is 570 bp, and 189 amino acids are encoded. Under drought, salt, high temperature and low temperature stress and induction of hormones ABA and MeJA, CpCML25 is up-regulated to different degrees. And performing agrobacterium tumefaciens-mediated transformation on the overexpression vector to obtain the 35S:: CpCML25 / arabidopsis thaliana strain. Compared with a wild type, the overexpressed strain shows the phenomena that the flowering time is advanced, the plant height is lower, the rosette leaf length is smaller, the number of stem leaves and primary lateral branches is increased and the like. The overexpression CpCML25 improves the drought resistance of the arabidopsis thaliana, and reduces the salt tolerance and cold tolerance of the arabidopsis thaliana.

The application provides a method for identifying and screening cold tolerance of elephant grass and application thereof, and relates to the field of plant cultivation. The method mainly comprises the following steps: subjecting the standard elephant grass stem segments to variable temperature germination; subjecting the standard elephant grass stem segments to constant temperature low temperature stress; subjecting the stressed stem segments to recovery culture under suitable conditions; determining and calculating the low temperature germination, stress survival rate and recovery growth rate of the stem segments; and comprehensively evaluating the cold tolerance of the stem segments based on the weighted values of the three. The application overcomes the shortcomings of the prior art, can simulate the key low temperature stress and recovery process in the laboratory, and can realize rapid, reliable and batch preliminary identification and screening of the cold tolerance of a large amount of materials, so that the blind dependence on large-scale and long-period field identification is significantly reduced, the screening accuracy and breeding efficiency are effectively improved, and the breeding period is shortened.

The invention discloses a DNA molecular marker related to rice seedling stage cold-tolerant gene haplotype and application of the DNA molecular marker, and belongs to the technical field of functional molecular markers. The rice seedling stage cold-resistant gene OsCTS11 has three main linkage imbalance blocks. Through correlation analysis of low-temperature stress phenotypes and haplotypes, it is determined that Hap4 (GCACA) of LD BLOCK1, Hap3 (GAA) of BLOCK2 and Hap4 (GTTG) of BLOCK3 are dominant haplotypes. The KASP molecular marker developed on the basis of different haplotype SNP sites can be used for analyzing OsCTS11 genotypes in different rice materials, and breeding materials with seedling-stage cold resistance can be screened out through the molecular marker.

The present invention discloses an InDel9b molecular marker of the cold tolerance gene LTSS9b at the rice seedling stage and its application, which relates to the field of rice breeding. This molecular marker is located in the promoter region (-392 to -1541) of the cold tolerance gene LTSS9b (LOC_Os09g23540) at the rice seedling stage. The present invention provides a primer set for detecting the above InDel9b molecular marker, and a kit including the above primer set. The present invention also provides the application of the above InDel9b molecular marker in identifying the cold tolerance at the rice seedling stage and a method for identifying the cold tolerance at the rice seedling stage. The molecular marker provided by the present invention can screen out cold tolerance varieties at the rice seedling stage. In practical applications, only conventional PCR combined with electrophoresis is required, which has low cost, high throughput and good specificity, and is suitable for breeding and production practices, effectively solving the problem of long cycle existing in the conventional breeding methods of cold tolerance rice varieties.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

The invention discloses an application of an OsBGGP gene in regulating and controlling the cold tolerance of rice in a seedling stage. The invention provides an application of a substance for inhibiting the activity of a protein OsBGGP or inhibiting the expression of a nucleic acid molecule encoding the protein OsBGGP in the following A1) or A2): A1) reducing the cold resistance of plants; a2) cultivating plants with low cold resistance; according to the present invention, the gene engineering method finds that the OsBGGP regulates the rice seedling stage cold resistance, the method for regulating the rice seedling stage cold resistance is found, and the understanding on the rice cold resistance regulation is expanded.

The invention discloses a major QTL for regulating and controlling the cold tolerance of rice in the seedling stage, a molecular marker and application. Belongs to the technical field of rice breeding and molecular biology. The invention provides the major QTL for regulating and controlling the cold resistance of the rice in the seedling stage and the molecular marker thereof, the molecular marker is applied to breeding of the rice with strong cold resistance in the seedling stage, rice offspring can be screened, and the breeding efficiency of the excellent cold-resistant rice is greatly improved while the cost is saved.

This invention discloses an InDel marker, Pmct3, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker from the insertion / deletion sequence between the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:28249918 and Chr1:28249919 sites in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The present application relates to the field of biotechnology, and particularly relates to application of HAG18 protein and its coding gene in improving cold tolerance of corn. It is found in the present application that overexpression of HAG18 gene in corn can enhance the low-temperature tolerance of plants, and further, application of corn HAG18 protein or its coding gene or biological material containing the coding gene in any of the following aspects is proposed: (1) changing the cold tolerance of plants; (2) improving the survival rate of plants in a low-temperature environment; (3) breeding transgenic plants with improved cold tolerance; (4) improving plant cold-tolerant germplasm resources. The present application provides a new gene resource for breeding new low-temperature tolerant plant varieties.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to plant cold resistance and application of the molecular marker. On the basis of a genome version number V4, the molecular marker is located at the 379th site of the downstream of a corn chromosome 2 Zm00001d006860 gene translation start site ATG, the polymorphism is G / A, and the cold tolerance of a plant corresponding to the genotype A is remarkably superior to that of a plant corresponding to the genotype G. The molecular marker provided by the invention can be used for detecting the cold resistance of the plant, and auxiliary identification of the cold resistance of the plant can be realized by detecting the polymorphism of the molecular marker in the plant. The molecular marker provided by the invention can significantly improve the plant breeding efficiency, and has great application value and practical significance.