62 results about "Heat Shock Transcription Factor" patented technology

A method of identifying RNA ligands which bind to a target molecule by treating a first pool of RNA ligands that collectively bind more than one target under conditions effective to reduce the concentration or eliminate the presence of one or more predominate target-binding RNA ligands from the first pool of RNA ligands; amplifying the RNA ligands in the treated first pool, thereby forming a second pool of RNA ligands that is enriched in one or more non-predominate target-binding RNA ligands of the first pool but not the one or more predominate target-binding RNA ligands thereof; and identifying one or more predominate target-binding RNA ligands that are present in the second pool at a higher concentration than other target-binding RNA ligands. Oligonucleotides and kits which can be used in practicing the present invention are also disclosed, as are aptamers that bind to a heat shock factor protein and their use.

The present invention provides a novel RNA, designated herein as the “HSR1” (Heat Shock RNA), and its use together with translation elongation factor eEF1A in activation of heat shock transcription factor HSF. The invention further provides the use of HSR1 for generation of novel therapeutics for the treatment of cancer, inflammation, ischemia, neurodegeneration, age-related diseases, HIV infection, deafness, and related disorders.

The present invention relates to HSF activating compounds, methods for their discovery, and their research and therapeutic uses, as well as pharmaceutically acceptable salts, solvates, chelates, non-covalent complexes, prodrugs, mixtures (including both R and S enantiomeric forms and racemic mixtures thereof), and pharmaceutical Formulations thereof. In particular, the present invention provides compounds capable of facilitating HSF1 homotrimerization, and methods of using such compounds as therapeutic agents to treat a number of conditions associated with irregular HSF1 activity.

A disease treatment is provided by controlling the expression of a protein induced by a heat shock factor.

The novel compound benzo-1,3-dioxole provides an inhibitor of HSF activity or an inhibitor of inducing the production of a protein regulated by HSF, which inhibits the activity of a heat shock factor, a transcriptional regulatory factor, thereby in turn inhibiting transcription of a structural gene having a heat shock element sequenc in the gene region for transcriptional regulation into RNA, and thus inhibiting translation of the gene into a protein, and resulting in inhibition of inducing production of RNA or protein encoded by the gene. It also provides a drug for treating or preventing cancer through thermotherapy and a drug for treating or preventing stress diseases such as depression.

To provide a heat shock transcription factor (HSF) 2 binding factor, which can be involved in the transcriptional regulation of HSP70.2 playing an essential role in spermatogenesis; a DNA encoding the binding factor; an expression vector carrying the DNA; a transformant harboring the expression vector; a process for preparing a recombinant protein comprising the step of culturing the transformant; an antibody or a fragment thereof capable of specifically binding to the binding factor; an antisense DNA or antisense RNA complementary to the DNA; and an oligonudeotide probe or primer capable of specifically hybridizing to the DNA.

The invention belongs to the field of technology related to plant molecular biology and transgenosis. Concretely, the invention relates to verification of high temperature resistance function of the Arabidopis thaliana heat shock transcription factor gene HSFA 2, and the application of the gene in high temperature resistance plant new species cultivation. According to the invention, a model plant Arabidopis thaliana is used as material, and a HSFA gene T-DNA is inserted into a homozygous mutant lines CSS 849143 for researching high temperature resistance function of the HSFA2 gene in plants. The experiment result displays that the HSFA2 gene knock-out mutant appears a sensitive phenotype with heat shock stress, and survival rate, conductivity and osmotic pressure and other physiological indexes are significantly or highly significantly different from those of the wild type; The invention instructs that the HSFA2 gene plays an important effect in the signal transduction pathway of plant response low nitrogen stress, and predicts that the excessive expression of the gene can improve the heat resistance capability of plant and has a larger economic value in the plant stress-resistance molecular breeding.

The invention belongs to the fields of genetic engineering and molecular breeding, and relates to a sequence of a heat shock transcription factor gene TaHsfA2i of wheat and application of the gene sequence. The nucleotide sequence of the heat shock transcription factor gene of the wheat is shown as in SEQ ID No.1. The gene TaHsfA2i is acquired from a heat resistant variety Cang 6005 of the wheat through cloning, the expression is obviously improved because of heat stress, and moreover, the expression of a downstream stress resistance gene is activated. The gene genetically transforms a yeast and arabidopsis, and the heat resistance capability of the yeast and the arabidopsis is obviously improved. A heat shock transcription factor encoding gene of wheat plays an important role in cultivating a crop variety with strong heat resistance.

The invention discloses application of an arabidopsis gene ROI1 in the aspect of high temperature stress resistance of plants. An arabidopsis mutant roi1 sensitive to high temperature and oxidation stress is obtained through screening; and the ROI1 gene is obtained by separating the arabidopsis mutant roi1. Experiments show that the arabidopsis ROI1 gene is induced to express by high temperature stress, regulates generation of H2O2 in plant bodies, is combined with heat shock transcription factors HSF3, regulates the activity of the HSF3 and downstream signal molecules in a mode of phosphorylation regulation, and regulates and improves high temperature stress resisting reaction of the plants. Functional analysis and identification of the ROI1 provide basis of theory and method for obtaining new varieties of high temperature stress resisting plants.

In certain aspects, the present disclosure provides compositions and methods for increasing red blood cell and/or hemoglobin levels in a subject in need thereof. Subjects in need include, for example, subjects having anemia and/or ineffective erythropoiesis as a result of having reduced GATA-1, heat shock factor and/or NFE2 levels as compared to the levels in a reference population.

Plants having enhanced yield-related traits and a method for making the same The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a HSFA4 or HSFA5 (Heat Shock Factor of the class A4 or A5) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a HSFA4 or a HSFA5 polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs comprising the same, useful in performing the methods of the invention. Furthermore, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding an oligopeptide transporter protein (OPT4-like) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an OPT4-like polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The invention also provides hitherto unknown OPT4-like-encoding nucleic acids, and constructs comprising the same, useful in performing the methods of the invention. Furthermore, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding a plastochron2-like (PLA2-like) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a PLA2-like polypeptide.

The present invention is aimed to provide a bioassay system that can detect hazardous chemical substances or natural toxic substances in the environment, inclusive of the unknown homologs, with high sensitivity, simplicity and speed. The present invention is a method for detecting a hazardous chemical substance or a natural toxic substance, which comprises culturing a cell in a test sample-containing medium and then measuring a reporter gene protein inducing activity. Said cell is obtained by transferring a promoter containing a heat shock factor binding DNA sequence and a transcriptional regulatory sequence necessary on an occasion of stress induction as a transcriptional regulatory factor binding site, as well as a reporter gene under the control of said promoter into a chromosome, said reporter gene being connected, on the downstream side thereof, to the SV40pA signal without any intervening intron.

The present invention relates to HSF activating compounds, methods for their discovery, and their research and therapeutic uses. In particular, the present invention provides compounds capable of facilitating HSFl activation, and methods of using such compounds as therapeutic agents to treat a number of conditions associated with protein misfolding.

The invention belongs to the fields of genetic engineering and molecular breeding and discloses a promoter of cotton heat shock transcription factor GhHsf 39 genes and application of the promoter. The nucleotide sequence of the promoter is shown as SEQ ID NO:1. When a plant is subjected to abiotic stress, the expression of target genes can be regulated by pghhsf39. When the pghhsf39 sequence cloned from upland cotton is subjected to different abiotic stress stimulus, the expression quantity of the GhHSF 39 genes can be increased. The pghhsf39 promoter disclosed by the invention is connected with the target genes so as to form an expression cassette, and transgenic plants subjected to adversity stress to regulate the expression of the target genes can be obtained. The invented pghhsf39 provides an important promoter element for researching plant stress resistance and disease resistance. Therefore, the promoter has wide application values.

The invention belongs to plant molecular biology and transgenosis related technical field, and specifically relates to verification of the high temperature resistance function of the Arabidopsis thaliana heat shock transcription factor gene HSFA5, and application of the gene in breeding new varieties of high temperature resistant plants. In the invention, the model plant Arabidopsis thaliana is adopted as the material, and T-DNA of the HSFA5 gene is inserted into a homozygous mutant strain salk_004385 to study the function of the HSFA5 gene in the aspect of plants' high temperature resistance. Experimental results show that: the HSFA5 gene knockout mutant shows a heat resistant phenotype under heat shock stress, and its survival rate is significantly higher than that of a wild type; and the HSFA5 gene plays an important role in a signal transduction pathway of plants' stress response to heat shock stress, and it is estimated that overexpression of the gene can improve heat resistance of plants and has great economic value in molecular breeding of plant stress resistance.

The invention provides a method of inducing a protective response in cells by activating a heat shock response, via heat shock transcription factor (HSF), without stressing the cells. The invention is based on the surprising discovery that HSF can be activated in non-stressed cells by disrupting interaction with the repressive cognate protein, Hsc70. As described herein, non-steroidal anti-inflammatory drugs (NSAID), including salicylic acid and ibuprofen, can stimulate HSF DNA binding, Hsp70 transcription and Hsp70 protein synthesis in non-stressed cells. By activating HSF, the degenerative effects of certain neurodegenerative proteins such as polyglutamine repeat proteins (Huntington's disease) and the α-synuclein protein (Parkinson's disease) can be offset. The invention thus provides a method of ameliorating symptoms of neurodegenerative disease.

The invention provides a novel biomedical application of a heat shock factor 2 binding protein (HSF2BP) as a liver cancer marker. The invention discloses an application of quantitative detection of HSF2BP mRNA and a protein in preparation of a liver cancer diagnosis or prognosis monitoring reagent, and particularly relates to an application of HSF2BP mRNA or protein expression increasing as a liver cancer prediction index or a poor prognosis index. The increased expression of the HSF2BP mRNA or protein in serum is identified through the real-time quantitative PCR, Western Blot method and immunohistochemical method, so that a diagnostic marker and a prognosis evaluation index of liver cancer are obtained and the application has an important clinical application value.

The invention discloses a plant expression vector of an arabidopsis heat shock factor gene AtHsfA1d, and the application thereof, belonging to the field of genetic engineering. According to the invention, the plant expression vector pK2-35S-AtHsfA1d is constructed by the arabidopsis heat shock factor gene AtHsfA1d, and is transferred into the plant via Agrobacterium mediation, so that the absorption capacity and the tolerance of the plant to the methanal are improved, and own defects of low absorption capacity and low tolerance to the methanal of the plant are solved; the experimental resultsshow that, after being cultred for 7 days in a MS solid culture medium containing 6mmol/L methanal, the transgenic tobacco with over-expression of an AtHsfA1d transcription factor has greater fresh weight than that of the wild tobacco, and the methanal absorption speed of the plant of the transgenic AtHsfA1d tobacco in 4mM methanal is higher than that of the wild tobacco.

The invention discloses an application of a heat shock transcription factor 1 in regulating expression of a 15kDa selenoprotein. The application provided by the invention is specifically any one of the applications of the heat shock transcription factor 1: (a1) promoting expression of a Sep15 protein in a target cell; (a2) promoting transcription of a Sep15 gene; and (a3) enhancing the activity of a Sep15 promoter in the target cell. According to the application disclosed by the invention, a transcription factor HSF1 can be combined with the Sep15 promoter to adjust transcription of the Sep15 gene and accelerate expression of the Sep15 protein. Under a heat shock and fever of a physiological status, the transcription factor HSF1 can start transcription of Sep15 and improve expression of Sep 15 so as to participate the physiological process and help the protein being corrected folded or help the protein being unfolded and delivered to other proteins to help the proteins to be folded correctly. Under endoplasmic reticulum stress, the HSF1 also can promote expression of Sep 15 to participate protein quality control of endoplasmic reticulum so as to further promote cell survival. The application disclosed by the invention is of important significance in preventing and/or treating endoplasmic reticulum stress related diseases.

The present invention belongs to the biotechnological field and relates to a human myocardial protection gene heat shock transcription factor (HSF1) and a sieving method and a purpose thereof. The present invention adopts the method of ''death trap'' that the gene which resists myocardial cell death is sieved from human cardiac gene, and the human myocardial protection gene heat shock transcription factor 1 is cloned from the obtained gene. The result of transfection cell and animal experiment shows that the obtained human myocardial protection gene has the function of resisting cell death and also has the function of resisting ischemia, protecting myocardial cell and preventing and remedying the heart failure occurrence and development inside the heart of a living organism. The present invention can prepare for the medicine for remedying the ischemic heart disease and preventing the cardiac insufficiency and provides a novel target gene for the further function research and the gene engineering medicine development, which has important meaning for the early prevention and treatment of the heart failure and the research and the development of the related medicines and measures.

The invention belongs to the plant molecule biology and transgenic related technical field, and specifically relates to verification of high temperature resistance of arabidopsis thaliana heat shock transcription factor gene HSFA3 and applications of the gene in breeding of new high temperature resistance varieties. According to the present invention, a model plant arabidopsis thaliana is adopted as a material, and HSFA3 gene T-DNA is adopted to insert into a homozygosis mutant line salk_011131 so as to research functions of the HSFA3 gene in the field of high temperature resistance of plants, wherein experiment results show that the HSFA3 gene knocked out mutant presents a heat resistance phenotype under heat shock stress, and a root length and above-ground biomass of the HSFA3 gene knocked out mutant are significantly more than a root length and above-ground biomass of the wild type, such that the HSFA3 gene provides important effects in a signal transduction pathway of the plant response to heat shock stress, and it can be predicted that overexpression of the gene can increase heat resistance of the plant and can provide high economic values in plant stress tolerance molecule breeding.