Liver cancer tumor marker heat shock factor 2 binding protein and application thereof

A tumor marker and binding protein technology, which is applied in the field of heat shock factor 2 binding protein, a tumor marker of liver cancer, can solve the problems of no published research on malignant tumors and less HSF2BP.

Active Publication Date: 2020-03-17
崇好科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researchers to detect specific proteins called hsps on blood cells that are associated with hepatocellular carcinoma (HCC). By measuring these molecules' levels over time during disease development, they may help identify individuals who have high risk factors for developing this type of cancer. They also suggest ways to develop effective treatments against those identified by their gene products such as chemotherapy agents targeting certain enzyme(s) involved in metastasis process.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and effectiveness of detecting diseased tissue within the gastrointestinal tract (GI). Current techniques involve invasively accessing organs or blood vessels through surgery, but these procedures require expensive medical facilities and may result in patient discomfort due to pain caused by bleeding during their recovery period. There exists a need for novel molecular targets useful for earlier identification of livers affected by lung cancer without requiring costly surgeries.

Method used

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  • Liver cancer tumor marker heat shock factor 2 binding protein and application thereof
  • Liver cancer tumor marker heat shock factor 2 binding protein and application thereof
  • Liver cancer tumor marker heat shock factor 2 binding protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Real-time quantitative PCR detection of HSF2BP mRNA expression in liver cancer tissues and corresponding adjacent normal tissues.

[0036] Patients with primary liver cancer who were admitted to the First Affiliated Hospital of Xi'an Jiaotong University and underwent "hepatectomy" from October 2012 to December 2016 were randomly selected, and 99 pairs of hepatocellular carcinoma and adjacent normal liver tissues (at least Tumor edge 3cm). The entire collection and experimental testing process was certified by the Ethics Committee of the First Affiliated Hospital of Xi'an Jiaotong University. Informed consent was signed for all tissue samples before collection. After the tissue specimens were obtained, they were immediately stored in a -80° refrigerator.

[0037] 1. Extraction of total cellular RNA using Trizol reagent

[0038] Materials and reagents: Trizol reagent, chloroform, isopropanol, DEPC water, 75% ethanol.

[0039] experiment procedure:

[0040]...

Embodiment 2

[0059] Example 2: Detection of the expression of HSF2BP protein in liver cancer tissue and corresponding adjacent normal tissue by Western Blot

[0060] 1. Tissue protein extraction process:

[0061] 1. Obtain 30mg of tissue protein and put it into a 2ml homogenization tube, add 300ul of pre-cooled RIPA lysate respectively;

[0062] 2. Add steel balls and crush for 1 minute at high speed on the tissue crusher;

[0063] 3. Take out the steel ball, place it on ice together with the mixing tube, and fully crack for 30 minutes;

[0064] 4. After centrifuging at low temperature and high speed for 15 minutes, carefully obtain the supernatant and transfer it to a new 1.5ml centrifuge tube;

[0065] 5. Take 10ul protein sample, use BCA protein quantification method to quantify, and obtain the concentration of sample protein;

[0066] 6. Add the remaining protein samples to 5×loading buffer in proportion and vortex to mix;

[0067] 7. Protein samples were heat-treated in boiling wa...

Embodiment 3

[0081] Example 3: Immunohistochemical method to detect the expression of HSF2BP protein in liver cancer tissues and corresponding normal adjacent tissues

[0082] 1. Paraffin tissue section

[0083] 1. Place the clinical specimen tissue in 4% paraformaldehyde solution and fix it at room temperature for 24 hours;

[0084] 2. Rinse with PBS 2-3 times, place the tissue in 80% alcohol for 48 hours, 95% alcohol for 12 hours, absolute alcohol for 2 hours, and xylene for 2 hours to dehydrate;

[0085] 3. Immerse the tissue in wax for 30 minutes;

[0086] 4. After routine embedding, slice serially with a thickness of 5um;

[0087] 5. Place the slices in a 37°C incubator for 48 hours and store at room temperature.

[0088] 2. Immunohistochemical staining

[0089] 1. Dewax the paraffin-embedded sections in xylene for 2 times, 10 min each time;

[0090] 2. Gradient alcohol dehydration;

[0091] 3. Place the slices in methanol solution of 0.3% hydrogen peroxide for 15 minutes at roo...

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Abstract

The invention provides a novel biomedical application of a heat shock factor 2 binding protein (HSF2BP) as a liver cancer marker. The invention discloses an application of quantitative detection of HSF2BP mRNA and a protein in preparation of a liver cancer diagnosis or prognosis monitoring reagent, and particularly relates to an application of HSF2BP mRNA or protein expression increasing as a liver cancer prediction index or a poor prognosis index. The increased expression of the HSF2BP mRNA or protein in serum is identified through the real-time quantitative PCR, Western Blot method and immunohistochemical method, so that a diagnostic marker and a prognosis evaluation index of liver cancer are obtained and the application has an important clinical application value.

Description

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Claims

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Application Information

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Owner 崇好科技有限公司
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