A kit for rapid detection of il28b SNP rs12979860 polymorphism

Abstract

The invention relates to a kit for rapidly detecting single nucleotide polymorphism (SNP) rs12979860 of an IL28B gene. By the kit, a specific primer is designed, the specific primer and template deoxyribonucleic acid (DNA) in a sample are subjected to an ordinary polymerase chain reaction (PCR), and the polymorphic forms (CC, CT and TT) of IL28B SNP rs12979860 of the sample is judged according to the result of the PCR, so that a result of therapeutic effect prediction is provided for the clinical antiviral therapy of hepatitis C patients. The kit is easy to operate, can detect the polymorphism quickly, is economic and effective, can be widely used for screening the therapeutic effect of the hepatitis C patients before antiviral therapy, is convenient for clinicians to formulate therapeutic schedules pertinently, has high clinical application value, and is particularly suitable for clinical promotion.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a kit for rapidly detecting IL28B SNP rs12979860 polymorphism, which is used for predicting the antiviral treatment effect of hepatitis C patients. Background technique

[0002] Hepatitis C infection is an infectious disease that seriously endangers human health, and its prevalence is distributed worldwide. According to WHO statistics, there are about 140 million to 170 million hepatitis C virus (Hepatitis C virus, HCV) infected people in the world. The infection rate of HCV in my country is about 3.2%, about 38 million HCV infected people, and about 35,000 new cases of hepatitis C every year.

[0003] Previous studies have shown that the treatment method and therapeutic effect of hepatitis C are closely related to the virus genotype. Patients with genotype 1 and genotype 4 are treated with pegylated interferon and ribavirin (PegIFN / RBV)48 weeks; genotype 2 and genotype 3 patients we...

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