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Kit for detecting alpha 1-acidoglycoprotein by using immunity transmission turbidity method

An immune transmission turbidimetry and acid glycoprotein technology, which is applied in the field of medical immunology in vitro diagnosis, can solve the problems of lack of test running time, batch-to-batch difference, high repeatability, and low degree of automation, and achieve high clinical application value and easy operation Simple, Highly Accurate Effects

Active Publication Date: 2013-05-01
潍坊三维生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many deficiencies in these technologies, such as the need for special equipment, the need for pretreatment of samples, and the inability to perform batch detection and analysis on fully automatic biochemical analyzers, etc.
Radioimmunoassay has problems such as radiation exposure and pollution
Enzyme-linked immunoassay is widely used, but this method is a heterogeneous immunoassay system, and the determination process is cumbersome, time-consuming, and lacks unit test running time; the degree of automation is not high, and the batch-to-batch difference and repeatability are relatively large; it needs to be equipped with A variety of specialized equipment increases the cost to a certain extent

Method used

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  • Kit for detecting alpha 1-acidoglycoprotein by using immunity transmission turbidity method
  • Kit for detecting alpha 1-acidoglycoprotein by using immunity transmission turbidity method
  • Kit for detecting alpha 1-acidoglycoprotein by using immunity transmission turbidity method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation of reagents:

[0040]

[0041] Reagent R2:

[0042]

[0043] Calibrator:

[0044]

[0045] A corresponding amount of α1-acid glycoprotein antigen was added to the above solution according to the required concentration of the calibrator to prepare the α1-acid glycoprotein calibrator. The calibrator can be a high-concentration single-point calibrator, diluted with normal saline to form 6 reference calibrators with different concentrations, or can be directly prepared into 6 reference calibrators with different concentrations. In this example, 6 reference calibration products with different concentrations were prepared, namely 0g / L, 0.15g / L, 0.31g / L, 0.62g / L, 1.24g / L, and 2.50g / L. Then use a 0.22 μm filter membrane to filter and sterilize, and store at 2-8°C.

Embodiment 2

[0047] 1. Preparation of reagents:

[0048] Reagent R1:

[0049]

[0050] Reagent R2:

[0051]

[0052] Calibrator:

[0053]

[0054]A corresponding amount of α1-acid glycoprotein antigen was added to the above solution according to the required concentration of the calibrator to prepare the α1-acid glycoprotein calibrator. In this example, a high-concentration single-point reference calibrator was selected, the concentration of α1-acid glycoprotein antigen was 2.7 g / L, and then sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C. When used, it was diluted with normal saline to form 5 reference calibration products with different concentrations, one of which was not added with α1-acid glycoprotein antigen, as a blank reagent, the concentrations of α1-acid glycoprotein antigen were 0g / L, 0.17g / L, 0.34 g / L, 0.68g / L, 1.35g / L, 2.70g / L.

Embodiment 3

[0056] 1. Preparation of reagents:

[0057] Reagent R1:

[0058]

[0059] Reagent R2:

[0060]

[0061] Calibrator:

[0062]

[0063] A corresponding amount of α1-acid glycoprotein antigen was added to the above solution according to the required concentration of the calibrator to prepare the α1-acid glycoprotein calibrator. In this example, 6 reference calibration products with different concentrations were prepared, namely 0g / L, 0.15g / L, 0.31g / L, 0.62g / L, 1.24g / L, and 2.50g / L. Then use a 0.22 μm filter membrane to filter and sterilize, and store at 2-8°C.

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Abstract

The invention discloses a kit for detecting alpha 1-acidoglycoprotein by using an immunity transmission turbidity method. A reagent R1 comprises 10-500 mM buffer liquid, a 0.5-50 g / L surfactant, a 5-50 g / L electrolyte, a 2-100 g / L high molecular accelerant, a 5-50 g / L stabilizing agent and a 1-10 g / L preservative; a reagent R2 comprises 10-500 mM buffer liquid, a 150-300 g / L anti-human alpha 1-acidoglycoprotein antibody, a 5-50 g / L electrolyte and a 1-10 g / L preservative; and a standard product comprises 10-500 mM buffer liquid, a 5-50 g / L alpha 1-acidoglycoprotein antigen, a 5-50 g / L stabilizing agent, a 1-10 g / L preservative and a 0.01-10 g / L antioxidant. The kit has the advantages of simplicity and fastness in use, remarkably improved detection efficiency and stable reagent, can satisfy the requirement of clinical and quick high throughout sample detection, and is suitable for clinical promotion.

Description

technical field [0001] The invention relates to the field of in vitro medical immunodiagnosis, in particular to a kit for detecting α1-acid glycoprotein by immunoturbidimetry. Background technique [0002] α1-acid glycoprotein, with a molecular weight of about 40,000, is a non-specific acute phase reaction protein, and also the glycoprotein with the highest sugar content (about 45% sugar content) and the strongest acidity (PI is 2.7-3.5) in human serum . α1-acid glycoprotein is mainly produced by liver macrophages and granulocytes, and can also be synthesized by cancer cells. [0003] α1-acid glycoprotein is currently a relatively sensitive marker of inflammation in the acute phase, and is one of the acute phase reactants. Its response to inflammation and infection is earlier than changes in body temperature and white blood cell count, so it is widely used clinically: (1) Increased content of α1-acid glycoprotein: Under pathological conditions, interleukin-1 stimulates pha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31
Inventor 宿明明王庆国王爱龙
Owner 潍坊三维生物工程集团有限公司
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