Arabidopis thaliana heat shock transcription factor gene HSFA 2 and coding protein and application thereof

A heat shock transcription factor, Arabidopsis technology, applied in the fields of plant molecular biology and transgenics, can solve problems such as difficulty, incomprehension in theoretical and technical research, and increase in gene function.

Inactive Publication Date: 2013-09-04
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, no detailed reports on the physiological responses of the HSFA2 gene to heat stress have been reported so far, which makes it d...

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  • Arabidopis thaliana heat shock transcription factor gene HSFA 2 and coding protein and application thereof
  • Arabidopis thaliana heat shock transcription factor gene HSFA 2 and coding protein and application thereof
  • Arabidopis thaliana heat shock transcription factor gene HSFA 2 and coding protein and application thereof

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with experimental methods and data.

[0017] 1. Arabidopsis seedling culture:

[0018] Wild-type and mutant seeds were sterilized by soaking in 0.5% sodium hypochlorite solution for 10 min, and the sterilized seeds were placed in a 4°C refrigerator for vernalization for 2 days, and then spread on MS (Murashige and Skoog) solid medium containing 1% agar. Germinate and grow in a light incubator at 22°C.

[0019] 2. Homozygous mutant identification:

[0020] DNA extraction: All experimental drugs used were purchased from Zhongke Ruitai Bioengineering Co., Ltd. Take about 0.5g fresh leaves of Arabidopsis thaliana, grind them into a paste, add 400μl SDS extract (0.5% SDS (W / V); 200mM Tris-HCl, pH=8.0; 250mM NaCl; 25mM EDTA); centrifuge at 12,000r / min 3min; take the supernatant, add an equal volume of isopropanol to gently shake, let stand for 30min; centrifuge at 13,000r / min for 5min; discard the superna...

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Abstract

The invention belongs to the field of technology related to plant molecular biology and transgenosis. Concretely, the invention relates to verification of high temperature resistance function of the Arabidopis thaliana heat shock transcription factor gene HSFA 2, and the application of the gene in high temperature resistance plant new species cultivation. According to the invention, a model plant Arabidopis thaliana is used as material, and a HSFA gene T-DNA is inserted into a homozygous mutant lines CSS 849143 for researching high temperature resistance function of the HSFA2 gene in plants. The experiment result displays that the HSFA2 gene knock-out mutant appears a sensitive phenotype with heat shock stress, and survival rate, conductivity and osmotic pressure and other physiological indexes are significantly or highly significantly different from those of the wild type; The invention instructs that the HSFA2 gene plays an important effect in the signal transduction pathway of plant response low nitrogen stress, and predicts that the excessive expression of the gene can improve the heat resistance capability of plant and has a larger economic value in the plant stress-resistance molecular breeding.

Description

Technical field: [0001] The present invention relates to the technical field of plant molecular biology and transgenics, in particular to the verification of the new function of HSFA2, a member of the Arabidopsis heat shock transcription factor (Heat Shock Transcription Factors, HSFs) gene family, and the application of the gene in new varieties of high temperature resistant plants. applications in cultivation. Background technique: [0002] The change of temperature has an important impact on the growth and development of plants. Appropriate temperature can ensure or promote the normal growth and development of plants, while unsuitable temperature conditions will inhibit the growth of plants and even cause plant death. Since the 20th century, with the rapid growth of world population and the continuous deepening of industrialization, the greenhouse effect has become more and more serious, and the gradual increase of global temperature has become an important limiting factor...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 徐江徐小栋张少斌东方阳鲁黎明王西瑶迟志海洪雪麻艳超赵明丁在松付金东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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