37 results about "Antisense DNA" patented technology

A process is described for producing hybrid seed using male-sterile plants created by employing molecular techniques to manipulate antisense DNA and other genes that are capable of controlling the production of fertile pollen in plants. Transformation techniques are used to introduce constructs containing antisense DNA and other genes into plants. Said plants are functionally male-sterile and are useful for the production of hybrid seed by the crossing of said male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

The present invention relates to an RNAi-inducing nucleic acid molecule having a new structure and the use thereof, and more particularly to a novel nucleic acid molecule having a structure comprising a first strand, which is 24-121 nt in length and comprises a region complementary to a target nucleic acid, and a second strand which is 13-21 nt in length and has a region that binds complementarily to the region of the first strand, which is complementary to the target nucleic acid, so that the nucleic acid molecule inhibits the expression of a target gene with increased efficiency, and to a method of inhibiting the expression of a target gene using the nucleic acid molecule. The nucleic acid molecule structure of the present invention increases the efficiency with which the nucleic acid molecule inhibits the target gene. Alternatively, the nucleic acid molecule of the present invention can either increase the ability of the siRNA to bind to the target gene or cause synergistic cleavage, by introduction of antisense DNA, antisense RNA, ribozyme or DNAzyme, thereby increasing the efficiency with which the nucleic acid molecule inhibits the target gene. In addition, when the nucleic acid molecule according to the present invention is used, the efficiency with which the target gene is inhibited can be maintained for an extended period of time. Accordingly, the RNAi-inducing nucleic acid molecule of the present invention can be effectively used for the treatment of cancer or viral infection in place of conventional siRNA molecules.

It has been discovered that reducing, inhibiting or preventing the expression of immunosuppressive cytokines or tolergenic agents in antigen presenting cells improves the ability of the antigen presenting cell to promote an immune response. One embodiment provides a genetically engineered antigen presenting cell that has reduced or no expression of IL-10. Preferred antigen presenting cells are dendritic cells. Expression of IL-10 can be inhibited or blocked by genetically engineering the antigen presenting cell to express inhibitory nucleic acids that inhibit or prevent the expression mRNA encoding immunosuppressive cytokines. Inhibitory nucleic acids include siRNA, antisense RNA, antisense DNA, microRNA, and enzymatic nucleic acids that target mRNA encoding immunosuppressive cytokines. Immunosuppressive cytokines include, but are not limited to IL-10, TGF-β, IL-27, IL-35, or combinations thereof. Tolerogenic agents include but are not limited to indoleamine 2,3-dioxygenase.

To provide a heat shock transcription factor (HSF) 2 binding factor, which can be involved in the transcriptional regulation of HSP70.2 playing an essential role in spermatogenesis; a DNA encoding the binding factor; an expression vector carrying the DNA; a transformant harboring the expression vector; a process for preparing a recombinant protein comprising the step of culturing the transformant; an antibody or a fragment thereof capable of specifically binding to the binding factor; an antisense DNA or antisense RNA complementary to the DNA; and an oligonudeotide probe or primer capable of specifically hybridizing to the DNA.

This invention discloses a method for preparing an expression vector containing double antisense waxy genes and its application. The structure and characteristics of the expression vector are shown in figure (d). The expression vector comprises promoter sequence, antisense DNA fragment of the target gene, terminator sequence, and endonuclease-sensitive sequence or marker gene sequence. All the sequences are doubled along the same direction. The expression vector can be used for breeding Gramineae plants such as rice without resistance-screening gene and with low amylase content.

According to the present invention, a plant having an early flowering property is provided. Specifically, the present invention provides a transformed plant having a gene that suppresses flower-bud formation or the antisense DNA of this gene.

A protected 2′-deoxycytidine is purified by precipitating the protected 2′-deoxycytidine represented by general formula (3) in the form of a hydrated crystal from a solution containing the protected 2′-deoxycytidine and water, and by recovering the protected 2′-deoxycytidine:

wherein R1 represents a 4-methoxytrityl, 4, 4′-dimethoxytrityl, or triphenylmethyl group; and B1 represents a cytosine group having a protected amino group. The compound represented by general formula (3) is, in particular, a protected 2′-deoxycytidine represented by formula (4):

The 2′-deoxycytidine is used as a raw material for antisense DNA.

The present invention relates to inhibitor molecules of LEREPO4 or GliPR or respective functional homologues thereof including siRNAs, shRNAs, antisense RNAs, antisense DNA and dominant negative proteinaceous mutants of LEREPO4 or functional homologues thereof. The present invention also relates to pharmaceutical compositions and methods for preventing and/or inhibiting HIV infections by inhibiting the function of LEREPO4 or GliPR or respective functional ho mo logues thereof in vivo. Furthermore, the present invention relates to methods of treating, preventing or diagnosing AIDS and/or HIV infections in an individual. Moreover, the present invention relates to diagnostic methods to determine the susceptibility of HIV strains and isolates for such LEREPO4 or GliPR directed treatment.

The present invention relates to a method of screening a drug by using a disease-associated gene product, an antibody to the disease-associated gene product, an antisense DNA that suppresses the expression of the disease-associated gene, etc. A compound regulating the activity of a protein having an amino acid sequence which is the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:1, or its salt, a neutralizing antibody regulating the activity of the above protein, an antisense DNA, etc. can regulate the expression of the protein having an amino acid sequence which is the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:1 and, therefore, are usable as preventive/therapeutic agents for diseases, e.g., heart diseases, etc.

The invention discloses a plasmid for constructing an interference vector, a construction method thereof and application thereof. The invention provides a plasmid pFGC-NAP obtained by inserting a DNA presented by sequence 1 in a sequence list between an Stu I enzyme cutting site and an Asc I enzyme cutting site of a plasmid pFGC1008. The interference vector can be obtained by respectively inserting a plus-sense DNA fragment and an anti-sense DNA fragment into a multiple cloning site of the plasmid pFGC-NAP, wherein the plus-sense DNA fragment and the anti-sense DNA fragment are inverted repeat sequences designed for a target gene to be interfered and expressed. The plasmid constructs the interference vector capable of specially expressing a promoter during a seed development period, has high interference efficiency, can control a spatial temporal specificity expression, can be used for a specific gene expressed in silent plant seeds, and improves quality of oil crop seeds or other plant seeds.

The present invention provides a nucleic acid-polysaccharide complex that is highly stable and has reduced cytotoxicity in a high yield. That is, the present invention provides a nucleic acid-polysaccharide complex of an antisense DNA having polydeoxyadenine (dA) as a polysaccharide binding site and a polysaccharide having a β-1,3-glucan skeleton wherein at least part of phosphodiester links of the polydeoxyadenine is phosphorothioated (provided that the phosphodiester links of the antisense DNA and the polydeoxyadenine are not entirely phosphorothioated).

The invention belongs to the technical field of biological detection and relates to a method for in-vitro detection of miRNA based on ratiometric fluorescence of acridine orange and carbon dots. The method comprises the following steps of preparing a carbon dot which is subjected to fluorescence resonance energy transfer with acridine orange, taking the acridine orange as a donor, taking the carbon dot as a receptor, and taking an antisense DNA chain of miRNA to be detected as a probe; adding a to-be-detected miRNA solution into a DNA probe solution with a certain concentration, incubating at 37 DEG C for 90 minutes, adding an acridine orange solution, adsorbing for 10 minutes, adding a carbon dot solution, and standing for 10 minutes; and testing fluorescence intensity of the mixed solution by using a fluorospectro photometer and calculating the concentration of the miRNA to be detected. The method is simple and convenient to operate, high in sensitivity and good in repeatability and specificity, and is a reliable method for miRNA in-vitro detection.

The use of antisense oligodeoxyribonucleotides (ODNs) to inhibit translation of mRNAs promises to be an important means of controlling gene expression and disease processes. ODNs are about 20 nucleotides long, so hundreds of possible targets are available in a given mRNA. An elusive goal has been to efficiently predict the best in vivo antisense target without having to study a large pool of possible ODN sequences for each mRNA. It would be a breakthrough if ODN selection could be accurately guided by the application of sequence specific parameters to an mRNA sequence. The selection of the best ODN sequence is complicated since cellular uptake, conditions at the mRNA target site, non-sequence-specific effects, sequence redundancy, and mRNA secondary structures are difficult to predict. Thermodynamic parameters for nearest-neighbor (dimer) duplex stabilities, from in vitro studies, have not been adequate predictors of in vivo hybridization. The methodology of this application shows that it is possible to obtain parameters for in vivo motifs, which are defined as combinations of next-nearest-neighbors, that are correlated with efficient antisense targeting. These parameters can be used to identify mRNA sequences that are binding sites for effective antisense ODNs. Next-nearest-neighbor nucleotide parameters can be derived directly from cell culture inhibition data so that in vivo conditions are taken into account.

The invention provides a method for generating and selecting drug-sensitizing antisense DNA fragments. In one embodiment, the method includes identifying a gene of interest using knowledge of bacterial physiology, biochemistry, genetics, genomics, and other means. The method includes PCR amplification of a gene of interest using genomic DNA as a template; fragmentation of the DNA by sonication or other means; selecting DNA fragments no longer than 400 base pairs; ligating the DNA fragments into a suitable expression plasmid with a selectable marker; transforming the plasmids containing the DNA fragments into the organism from which the gene of interest originated; and selecting clones from transformed cells that show a phenotypic difference of the clone grown in the presence of the inducer relative to the phenotype in the absence of inducer.

Disclosed herein are antisense DNA of a sweetpotato expansin (IbExpansin) cDNA, a plant transformation vector carrying the same, and a method for increasing storage root production using the same. The transgenic sweetpotatoes prepared using the antisense DNA of IbExpansin cDNA have storage root production increased by up to one and half times. Thus, the gene is useful in the generation of highly productive transgenic storage roots for the increase of bioethanol production.