46 results about "Ratiometric fluorescence" patented technology

The invention discloses a preparation method and application of a granzyme B response near-infrared two-region ratiometric fluorescent probe (DCGA). The DCGA combines the NIR-II dual-emission fluorescence characteristic of rare earth ions with a ratio-type signal response mode, the sensitivity and signal stability of in-vivo fluorescence imaging are greatly improved, ultralow or enhanced F1060nm fluorescence signals are respectively shown before and after interaction with granzyme B (GzmB), F1525nm fluorescence signals are almost kept unchanged, and the DCGA can be used for detecting the in-vivo fluorescence imaging of the granzyme B (GzmB). Therefore, the self-calibration ratio fluorescence imaging GzmB activity change is realized. The DCGA is applied to tumor treatment of immunotherapy drugs, and responders and non-responders of immunotherapy can be distinguished in a non-invasive early stage through ratio fluorescence signals of the DCGA. Therefore, the fluorescent probe can be used as an effective GzmB active NIR-II ratio fluorescence imaging tool, and is expected to provide powerful support for immunotherapy research, precision medicine and personalized diagnosis and treatment.

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The invention discloses a preparation method of a portable paper-based ratiometric fluorescence sensor APBA-CsPbBr3 at-UiO-66 / Ru, which comprises the following steps: 1, adding lead bromide and cesium bromide into N, N-dimethylformamide, then adding 5-bromovaleric acid and an oleylamine ligand, and stirring; 2, perfluorooctyltriethoxysilane is added, and a precursor solution is obtained; 3, adding zirconium chloride and terephthalic acid into N, N-dimethylformamide to obtain a homogeneous solution; 4, after heating, UiO-66 is obtained; 5, stirring the precursor solution, APBA, UiO-66 and tris (2, 2-dipyridyl) ruthenium chloride hexahydrate to obtain a composite material; and 6, dropwise adding the composite material solution into the filter paper. The APBA modified material is adopted as a recognition unit, the APBA modified material and the paper base construct a fluorescence sensing platform, visual quantitative analysis of oxytetracycline in water is achieved, and the device is convenient to carry, low in detection limit, high in anti-interference performance, easy and convenient to operate and suitable for on-site rapid detection of oxytetracycline.

This invention discloses a dual-emission molecularly imprinted ratiometric fluorescence sensor, its preparation method, and its application in the detection of chlortetracycline, belonging to the field of chemical sensor technology. The preparation method of this sensor includes the following steps: preparing silica-quantum dot composite nanoparticles; preparing a molecularly imprinted polymer using salicylamide as a template molecule; and using a post-mixing strategy to mix the molecularly imprinted polymer and silica-quantum dot composite nanoparticles in HEPES buffer to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The advantages of this invention are: the dual-emission molecularly imprinted ratiometric fluorescence sensor provided by this invention exhibits rich fluorescence color changes, enabling rapid, highly sensitive, and visual detection of chlortetracycline; and the entire detection process is simple, convenient, and fast, making it suitable for widespread application in practical applications.

The invention discloses a label-free proportional type luminous aptamer biosensor for staphylococcus aureus enterotoxin A (SEA). The label-free proportional type luminous aptamer biosensor comprises the following steps: (1) advanced evolution screening of a complex matrix high-adaptability aptamer; (2) rationally cutting the aptamer assisted by computer simulation; (3) a construction strategy of the label-free proportional biosensor; (4) a label-free proportional SEA bifunctional biosensor sequence; (5) optimizing conditions of the label-free proportional SEA biosensor; and (6) SEA detection. The design principle is that fluorescence of SEA and ThT is excited to different degrees on the basis of a competitive binding effect between an SEA target and a small molecule nucleic acid dye thioflavin T (ThT) as well as a luminous nucleic acid aptamer, so that the ratio of fluorescence signals of the SEA and the ThT is in a linear relationship with the target concentration, and finally, rapid, label-free and low-cost proportional fluorescence detection of the SEA is realized.

This invention discloses a GSH-functionalized carbon dots / rhodamine B ratiometric fluorescence system, its construction method, and its application in nitrite detection. GSH-CDs are synthesized via a one-step hydrothermal method by mixing citric acid, o-phenylenediamine, glutathione, and hydrochloric acid in water. Unreacted precursors and small molecules are removed by purification, and the GSH-CDs are obtained by freeze-drying. The ratiometric fluorescence system is constructed by mixing rhodamine B, GSH-CDs, and an acidic buffer solution. The fluorescence intensity ratio F of GSH-CDs to rhodamine B is established. 460 / F 590 With nitrite (NO2) ‑ A calibration curve between concentrations was obtained, and the NO2 in the actual sample was experimentally detected using the spiked recovery method. ‑ Content. Under acidic conditions, with NO2... ‑ With increasing concentration, the GSH-CDs / RhB fluorescence intensity ratio (I 460 / I 590 Significant changes occurred, with the fluorescence intensity ratio compared to NO2. ‑ There is a good linear relationship between concentrations. Compared with the detection platform based on GSH-CDs, the ratio fluorescence system provided by this invention has a lower detection limit and significantly higher sensitivity.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a fluorescent probe for detecting pyrophosphoric acid or pyrophosphate based on ratiometric fluorescence, a preparation method and application. According to the fluorescent probe, cyclotriphosphazene is taken as a core platform, a naphthalimide-triphenylamine luminophore and a terpyridine recognition unit are introduced, a multi-arm fluorescent probe is integrally formed, coordination terpyridine interacts with metal ions Zn < 2 + >, fluorescence signals are induced to change, and therefore sensitive detection on PPi is achieved. The fluorescent probe has the advantages of quick response, high selectivity, interference resistance and low cost, and has important application value in the fields of environmental science, biological science and the like.

The present invention provides a novel metal nanomaterial, specifically a silver cluster metal nanomaterial, a preparation method, and its application. Over a wide temperature range, there is a one-to-one correspondence between the emission intensity at 545 nm and the emission intensity at 505 nm. This property demonstrates the material's superior ratiometric fluorescence thermometry performance. The silver cluster metal nanomaterial can be used as a nanofluorescence thermometer and has great application potential in high-tech fields such as biomedicine (such as cell metabolism, pathology, medical imaging, and drug delivery) and electronic component manufacturing.

This invention belongs to the field of food and agricultural product safety testing technology, specifically relating to a rapid detection method for parathion and its application. Based on the parathion-induced blue shift in fluorescence of silicon nanoparticles, ascorbic acid is reacted with 3-aminopropyltriethoxysilane to generate green fluorescent silicon nanoparticles. During the formation of silicon nanoparticles, parathion can embed itself into the porous structure of the silicon nanoparticles through host-guest interactions, causing a change in the excited state of the silicon nanoparticles. This affects the fluorescence emission of the silicon nanoparticles, resulting in a blue shift in the fluorescence emission peak, changing from green fluorescence to blue fluorescence, specifically manifested as an increase in fluorescence intensity at 422 nm and a decrease in fluorescence intensity at 520 nm. By establishing the relationship between ratiometric fluorescence signal or fluorescence color change and parathion concentration, specific detection of parathion is achieved. This invention requires no enzymes or antibodies for parathion detection, is inexpensive, fast, easy to operate, highly sensitive, and has good specificity.

This invention provides a method for constructing a donor-acceptor type perylene diimide-based porous conjugated polymer probe and detecting methamphetamine and amphetamine solutions. The method synthesizes donor-acceptor type perylene diimide-based porous conjugated polymer probes with different topologies by combining an electron acceptor perylene diimide derivative and an electron donor aggregation-induced emission fluorophore via a succinate coupling reaction. When used for the detection of methamphetamine and amphetamine, this system exhibits a clear ratiometric fluorescence response, with the fluorescence color gradually changing from yellow-green to orange and orange-red, demonstrating a significant ratiometric fluorescence response. It features fast response speed, high detection sensitivity, good selectivity, and no interference from other common structurally and property-similar substances, enabling the differentiation and identification of methamphetamine and amphetamine.

The invention belongs to the technical field of food detection, and particularly relates to a molecular imprinting ratiometric fluorescence sensor as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, preparing SiO2 microspheres by adopting an improved Stober method, then respectively preparing a bCDs (at) SiO2 composite material and rCDs by adopting a one-step hydrothermal method, and finally, preparing the molecular imprinting ratio fluorescence sensor bCDs (at) SiO2amp by taking the bCDs (at) SiO2 composite material and the rCDs as fluorescence sources and adopting a sol-gel method. The sensor can be used for high-selectivity and high-sensitivity detection of the ethyl carbamate.

The present invention provides a fluorescent probe and its preparation method and application. The structural formula of the fluorescent probe is as follows: The preparation method is: 7-N,N-diethylamino-2H-chromene-3-aldehyde, 4-methyl-N-(4'-boronic acid pinacol benzyl) pyridine bromide, a catalyst and a solvent are stirred, mixed and heated under reflux for reaction, and then filtered and recrystallized to obtain a fluorescent probe product. The probe has good water solubility, biocompatibility and is positively charged. The solution color is purple-red, the absorption peak is at 540nm, and the fluorescence peak is at 720nm (near-infrared region), which can be used for near-infrared biological fluorescence imaging. The probe has the property of realizing ratiometric colorimetry and ratiometric fluorescence response to peroxynitrite. After reacting with peroxynitrite in a CH3CN-pbs (v / v=1:1, v / v, pH=7.4) solution, the color of the probe solution changes from purple-red to slightly yellow, A 410nm / A 540nm The probe has a linear relationship with the concentration of peroxynitrite in the range of 0 to 10 μM, with a detection limit of 95 nM. The red fluorescence of the probe gradually weakens at 720 nm and the bright yellow fluorescence gradually increases at 568 nm. 568nm / I 720nm The probe has a linear relationship with peroxynitrite concentration in the range of 0 to 16 μM, with a detection limit of 10 nM. The probe has the advantages of high sensitivity, low detection limit, and low background fluorescence interference.

This invention discloses the construction and application of a multifunctional ratiometric fluorescence sensing platform based on aggregation-induced emission nanozymes, belonging to the field of nanocomposite materials technology. This invention involves the stepwise synthesis of a zirconium-based aggregation-induced emission metal-organic framework (Zr-TCPE) and cadmium telluride quantum dots (CdTe QDs), followed by the preparation of a Zr-TCPE / CdTe ratiometric fluorescent nanozyme using an electrostatic self-assembly method. In this composite material, Zr-TCPE exhibits organophosphorus hydrolase-like activity, specifically catalyzing the hydrolysis of para-oxygen and phosphorus, resulting in specific quenching of its fluorescence at 475 nm. Meanwhile, the fluorescence of CdTe QDs remains stable at 585 nm, enabling self-calibrated detection through the ratio of the two fluorescence signals. This invention features mild synthesis conditions and simple operation. The constructed sensing platform has a detection range spanning four orders of magnitude, a low detection limit, strong anti-interference ability, and excellent long-term stability over 24 days. It can be effectively used for rapid and highly sensitive detection of para-oxygen and phosphorus in complex samples of organophosphorus pesticides.

This invention proposes a ratiometric fluorescent probe molecule for detecting peroxynitrite, its preparation method, and its application, aiming to solve a problem in the technical field of fluorescent sensing materials. The ratiometric fluorescent probe molecules of this invention are HPIP-T-BnB and HPIP-K-BnB. The structural formulas of HPIP-T-BnB and HPIP-K-BnB are: [Insert structural formula here], and the structural formulas of HPIP-K-BnB are: [Insert structural formula here]. The preparation method includes the following steps: 4'-(diphenylamino)-4-(imidazo[1,2-a]pyridin-2-yl)-[1,1'-biphenyl]-3-ol or 4'-(9H-carbazole-9-yl)-4-(imidazo[1,2-a]pyridin-2-yl)-[1,1'-biphenyl]-3-ol undergoes a nucleophilic substitution reaction with 2-(4-(bromomethyl)phenyl)-4,4,5,5-tetramethyl-1,3,2-dioxoborhecyclopentane to obtain HPIP-T-BnB and HPIP-K-BnB. The probes prepared in this invention can detect ONOO⁻ with high selectivity, high sensitivity, and quantification, and have been successfully applied to ratiometric fluorescence imaging of exogenous and endogenous ONOO⁻ in live cells. This provides a reliable and crucial tool for mechanism research and drug development in related diseases.

The invention relates to a dual-signal probe based on ratiometric fluorescence and visualization and application thereof, and relates to the technical field of ratiometric fluorescence sensing. The double-signal probe comprises nitrogen-doped carbon dots and o-phenylenediamine. The nitrogen-doped carbon dots are synthesized through a one-step hydrothermal method, the nitrogen-doped carbon dots and o-phenylenediamine form the ratiometric fluorescent and visual dual-signal sensor, the redox reaction between copper ions and o-phenylenediamine and the fluorescence resonance energy transfer of the reaction product oxidized o-phenylenediamine to the nitrogen-doped carbon dots are ingeniously utilized, and the fluorescence resonance energy transfer of copper ions and o-phenylenediamine to the nitrogen-doped carbon dots is greatly improved. Therefore, extremely sensitive visual detection on the copper ions is realized, and the method is applied to sensitive visual detection on the content of the copper ions in industrial ethylene glycol and has a relatively high application prospect.

The invention discloses a method for detecting total phosphorus concentration in an anaerobic fermentation system by using a ratiometric fluorescence sensor, the method is characterized in that the ratiometric fluorescence sensor is used for detecting total phosphorus in the anaerobic fermentation system, the adopted ratiometric fluorescence sensor is a lanthanide series metal organic framework suspension, and the lanthanide series metal organic framework is formed by europium ions and [1, 1 ': 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4 The compound is composed of 1, 1 ', 1'] terphenyl-3, 3 ', 5, 5'-tetracarboxylic acid. According to the method disclosed by the invention, the ratio fluorescence sensor has the advantages of high energy transfer efficiency, good fluorescence performance and the like, can realize rapid and accurate detection when being used for detecting the total phosphorus concentration in the anaerobic fermentation system, and has the advantages of short detection time, high detection sensitivity, large detection linear range, high detection precision, strong anti-interference capability and the like; the total phosphorus concentration of different stages in the anaerobic fermentation system can be timely and accurately obtained, so that effective data can be provided for accurate analysis and effective control of the anaerobic fermentation system.

This invention provides a colorimetric / fluorescence dual-mode sensor, its preparation method, and its application in histamine detection. The colorimetric / fluorescence dual-mode sensor comprises a metal-organic framework (HRP@AIE-MOF) loaded with horseradish peroxidase, hydrogen peroxide, and the chromogenic substrate o-phenylenediamine (OPD). The horseradish peroxidase (HRP) in the HRP@AIE-MOF oxidizes colorless OPD to yellow 2,3-diaminophenazine (DAP), producing a UV absorption peak at 425 nm and a strong fluorescence emission peak at 555 nm. The HRP@AIE-MOF also exhibits a strong fluorescence emission peak at 450 nm, which, together with the fluorescence signal of DAP, forms a ratiometric fluorescence sensor. This invention integrates the excellent catalytic ability of a natural enzyme with the luminescent properties of AIE-MOF into a single system, achieving highly sensitive histamine detection.

The invention discloses a ratio type fluorescent probe and application thereof in distinguishing and detecting aflatoxin B1 and aflatoxin B2a, and belongs to the field of fluorescent probe detection. The probe is assembled by taking serum albumin (HSA) as a subject and dicyanoisophorone dye (MDM) as an object. Based on the combination principle difference that an MDM derivative and aflatoxin B1 tend to coexist in HSA and the MDM derivative and aflatoxin B2a tend to be replaced, the ratio-type fluorescent probe MDM-coated HSA disclosed by the invention can realize distinguished detection of B1 and B2a by utilizing spectral response sensitivity difference and fluorescence color signals. The probe can be used for quantitatively detecting aflatoxin B2a in a real food sample due to high sensitivity of B2a, a colorimetric signal generated by ratiometric fluorescence of the probe can be used for on-site qualitative rapid detection, and dual-wavelength response can be used for in-situ differentiated imaging of B1 and B2a in a biological system.

The invention discloses a method for specific detection of sodium dichloroisocyanurate in ratiometric fluorescence-colorimetric dual modes, and belongs to the technical field of ratiometric fluorescence-colorimetric dual-mode sensors and sodium dichloroisocyanurate detection. The invention specifically constructs a sensor for specific detection of sodium dichloroisocyanurate in a ratio fluorescence-colorimetric dual mode, designs a method for specific and high-sensitivity detection of sodium dichloroisocyanurate in combination with ratio fluorescence and colorimetric, and can construct linear detection models at 4.0-40.0 [mu] mol / L and 2.0-70.0 [mu] mol / L respectively. And a novel and feasible method is provided for detection of sodium dichloroisocyanurate.

The invention discloses a preparation method and application of a hydrogen sulfide responsive NIR IIb ratiometric fluorescent nanoprobe. The dye is mainly composed of a specific hydrogen sulfide response dye IR-820 dihexanoic acid and lanthanide doped down-conversion nanoparticles with NIR IIb emission characteristics, and the preparation method comprises the following steps: synthesizing a nanocrystal nucleus by pyrolyzing lanthanide acetate, and preparing core-shell structure nanoparticles with multiband absorption characteristics; and modifying the surfaces of the PEI functionalized lanthanide nanoparticles with IR-820 dihexanoic acid in a covalent coupling manner, and centrifuging and washing to obtain a probe product. The nanoprobe disclosed by the invention combines a ratio-type fluorescence response mechanism and a deep tissue penetration characteristic of an NIR IIb window, effectively overcomes background interference and an autofluorescence effect of complex biomolecules in a plant body, remarkably improves sensitivity and spatial resolution of hydrogen sulfide detection, provides a novel nano optical tool, and has a very good application prospect.

A method for dual-mode detection of beta-lactam antibiotic residues based on laccase-like activity nano-enzyme Cu-MOF is characterized in that a copper metal organic framework (Cu-MOF) with laccase-like activity and blue fluorescence is prepared by adopting a hydrothermal method, color change is generated in the process of oxidizing o-phenylenediamine (OPD) to generate a yellow product 2, 3-diaminophenazine (DAP), and the color change is changed in the process of oxidizing o-phenylenediamine (OPD) to generate the yellow product 2, 3-diaminophenazine (DAP). And dual-mode signal output of colorimetric and ratiometric fluorescence is realized. When the BLs exist, the BLs and OPD are competitively combined with Cu-MOF active sites, generation of DAP is inhibited, a colorimetric signal is weakened, blue fluorescence is recovered, and therefore high-sensitivity and high-selectivity detection of the BLs is achieved. According to the invention, a portable on-site quantitative detection platform is established by further combining smartphone image acquisition and RGB analysis technologies, is successfully applied to rapid and accurate detection of BLs residues in animal-derived foods such as milk and pork, and has the advantages of simplicity and convenience in operation, low cost, on-site applicability and the like.

A method for ratiometric detection of putrescine using PtPd NPs@TPE fluorescent nanozymes. This invention first utilizes mesoporous platinum-palladium nanoparticles (PtPd NPs) doped with tetraphenylethylene (TPE) to obtain fluorescent nanoparticles (PtPd NPs@TPE) with oxidase-like activity, which can produce blue fluorescence under 365 nm ultraviolet light excitation. Secondly, this invention utilizes the antioxidant properties of putrescine solution to inhibit the formation of 2,3-diaminophenazine (DAP), an oxidation product, during the oxidation of o-phenylenediamine (OPD) by PtPd NPs@TPE, thereby reducing the DAP content. Based on the relationship between putrescine concentration and the colorimetric intensity of DAP, and the ratio of the yellow fluorescence intensity of DAP at 552 nm to the blue fluorescence intensity of PtPd NPs@TPE at 442 nm, a novel ratiometric fluorescence method for detecting putrescine is constructed. This method can be used to detect actual samples such as dried bean curd, with a detection limit as low as 0.0025 μg / mL.

The invention discloses a multifunctional nano material fluorescence-colorimetric dual-mode food-borne pathogenic bacterium detection and killing method and a system thereof. According to the method, a metal organic framework material (MIL-53 (Fe)) with nano-enzyme activity is used as a signal switch and a generator, carbon nanodots (CDs) of a fluorescence indication and photo-thermal sterilization unit are combined to form a basic platform, and masking of an aptamer on a nano-enzyme active site and competitive release under triggering of target bacteria are used as a core mechanism. A colorimetric and ratiometric fluorescence double-signal channel is activated to realize self-verification high-precision detection; cDs in the system are used as a photo-thermal element, and in-situ photo-thermal sterilization is realized after detection. The platform design that detection objects can be switched by replacing the aptamers shows good universality and application prospects, and a sensitive and efficient new strategy is provided for detection and elimination of food-borne pathogenic bacteria.

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

The application provides a high-sensitivity ATP fluorescence detection method and system. The application determines the optimal ratio of luciferase and luciferin, configures a reaction mixture containing the optimal ratio of luciferase and luciferin, and ensures that a significantly enhanced fluorescence signal is generated under a low-concentration ATP condition. Based on the reaction mixture, the application applies microfluidic chip technology to control the reaction environment, fully mixes the reaction mixture and the sample to be detected in a dark environment, adjusts the reaction temperature by using a dynamic temperature control technology, and obtains a mixed reaction system. The application uses a high-sensitivity photomultiplier tube to capture the fluorescence signal generated by the mixed reaction system within a set time, separates and generates a pure target fluorescence signal, and measures the intensity of the pure target fluorescence signal. The application analyzes the intensity of the pure target fluorescence signal, calculates and generates the concentration value of ATP in the sample to be detected. The technical scheme provided by the application improves the sensitivity and accuracy of ATP detection.

The present invention belongs to the technical field of organic chemistry and polymer material chemistry, and discloses a method for preparing a novel copolymer material based on coumarin and rhodamine and its application. When the pH value of the solution changes from alkaline to acidic, the solution color changes from blue to yellow-green, and the emission wavelength red-shifts from 484nm to 554nm. The material can be used as a probe to detect in the extreme acid range of 2.24-2.64, and has potential applications in environmental monitoring as a naked eye probe. The polymer material M-Z-4-D not only realizes the ratio fluorescence detection of pH, but also provides a new idea for the development of subsequent ratio fluorescence probes.

This invention discloses an acridine-based fluorescent nanofilm, its preparation method, and its application in the fluorescence sensing detection of ethylenediamine, belonging to the field of gas phase sensing materials. The fluorescent nanofilm is prepared by the self-assembly reaction of an aldehyde-modified acridine derivative and calix[4]pyrroletetrahydrazine at the gas-liquid interface via acylhydrazone bonds. By controlling the precursor concentration and reaction time, this invention can achieve precise control of the nanofilm thickness. The prepared nanofilm has excellent self-supporting properties and a uniform porous network structure, effectively suppressing the aggregation-induced quenching effect. When this nanofilm is used as a fluorescent sensing material for the detection of ethylenediamine, it exhibits a ratiometric fluorescence response, with advantages such as fast response speed, rapid recovery, low detection limit, high selectivity, and good stability, which can meet the needs of rapid on-site visualization detection. The preparation method of this invention is simple, the reaction conditions are mild, and no template is required, which has important application prospects in the field of on-site visualization detection of ethylenediamine.

The invention relates to the technical field of fluorescence sensing detection, in particular to a molecularly imprinted ratiometric fluorescence sensor which comprises a response signal assembly and a reference signal assembly, the response signal assembly is molecularly imprinted polymers (MIPs) loaded with 7-hydroxycoumarin, and the reference signal assembly is CdTe-coated SiO2 quantum dots; the molecularly imprinted polymer is prepared by taking isopropyl methanesulfonate as a template molecule, ethylene glycol dimethacrylate (EDGMA) as a cross-linking agent and azodiisobutyronitrile (AIBN) as an initiator through a precipitation polymerization method. The defect that a single-emission fluorescence sensor is easily influenced by background noise, instrument fluctuation and environmental factors is effectively avoided, and the detection accuracy and reliability are improved.

This invention proposes a quantum dot@ZIF-8 composite material, its preparation method, and its applications, belonging to the field of functional nanomaterials and fluorescence sensing analysis and detection technology. This invention involves mixing CdTe quantum dots (CdTe QDs) with red fluorescence emission and carbon quantum dots (C QDs) with blue fluorescence emission in a specific ratio, followed by in-situ self-assembly growth of ZIF-8, resulting in a quantum dot@ZIF-8 composite material with dual fluorescence signal emission. The quantum dot@ZIF-8 nanocomposite material prepared by this method not only possesses the fluorescence properties of quantum dots but also largely retains the original structure and properties of ZIF-8, making it a simple and effective method for preparing composite materials with excellent fluorescence performance. This quantum dot@ZIF-8 composite material can be used to prepare ratiometric fluorescence sensing probes for the visual detection of glucose with high sensitivity.