Preparation method and application of a bsa-au/ag NCs/OPD/HRP ratiometric fluorescent sensor

A fluorescent sensor, proportional technology, used in the field of analytical chemistry to achieve good specificity, avoid potential interference, and high sensitivity

Active Publication Date: 2021-05-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the development of a ratiometric fluorescent sensor strategy is of great significance, and the detection of uric acid in serum by a ratiometric fluorescent sensor has not been reported.

Method used

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  • Preparation method and application of a bsa-au/ag NCs/OPD/HRP ratiometric fluorescent sensor
  • Preparation method and application of a bsa-au/ag NCs/OPD/HRP ratiometric fluorescent sensor
  • Preparation method and application of a bsa-au/ag NCs/OPD/HRP ratiometric fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028] (1) Preparation of bovine serum albumin-gold-silver alloy nanocluster (BSA-Au / Ag NCs) aqueous solution:

[0029] Mix 4mL of chloroauric acid (10mM) and 1mL of silver nitrate (10mM) (making the gold-silver molar ratio 4:1; keep the total volume of chloroauric acid and silver nitrate at 5mL), add to 5mL of bovine serum albumin aqueous solution (0.75mM), vigorously stirred and mixed for 5 minutes, adjusted the pH of the solution with sodium hydroxide, and then placed it in a constant temperature oil bath at 37°C for 12 hours. After the reaction was completed, it was purified by ultrapure water dialysis for 48 hours to obtain Bovine serum albumin--aqueous solution of gold-silver alloy nanoclusters;

[0030] (2) Establish BSA-Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor:

[0031] The BSA-Au / Ag NCs aqueous solution, OPD, and HRP obtained in step (1) were added to phosphate buffer (10mM, pH=6.0) to obtain a BSA-Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor. Among them...

example 2

[0043] (1) bovine serum albumin--the preparation steps of the aqueous solution of gold-silver alloy nanoclusters are the same as in Example 1;

[0044] (2) Establish BSA-BSA-Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor system:

[0045] The BSA-Au / Ag NCs aqueous solution, OPD, and HRP obtained in step (1) were added to phosphate buffer (10mM, pH=6.0) to obtain a BSA-Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor. Among them, the concentration of BSA-Au / Ag NCs is 20nM, the concentration of OPD is 50μM; the concentration of HRP is 10ng / mL.

[0046] Join H 2 o 2 , get I 580 / I 690 =4.4201

example 3

[0048] (1) The preparation steps of the bovine serum albumin-gold-silver alloy nanocluster aqueous solution are the same as in Example 1;

[0049] (2) Establish BSA-BSA-Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor system:

[0050] The BSA-Au / Ag NCs aqueous solution, OPD, and HRP obtained in step (1) were added to phosphate buffer (10mM, pH=6.0) to obtain a BSA-Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor. Among them, the concentration of BSA-Au / Ag NCs is 20nM, the concentration of OPD is 200μM; the concentration of HRP is 10ng / mL.

[0051] Join H 2 o 2 , get I 580 / I 690 =7.4580

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Abstract

The invention belongs to the field of analytical chemistry and provides a preparation method and application of a BSA-Au / Ag NCs / OPD / HRP proportional fluorescence sensor. The invention includes (1) preparation of BSA‑Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor; (2) uricase catalyzes uric acid to generate H 2 o 2 ; (3) detect H with BSA‑Au / Ag NCs / OPD / HRP ratiometric fluorescent sensor 2 o 2 , so as to detect the uric acid content. Compared with the existing technology, the BSA‑Au / Ag NCs / OPD / HRP ratiometric fluorescence sensor not only has the advantages of short fluorescence sensor time, simple operation, no need to deal with early samples, non-toxicity, and low cost, but also has the advantages of single fluorescence sensor have higher sensitivity and accuracy. The level of uric acid in serum is closely related to human diseases, so it is of great significance to invent a proportional fluorescent sensor to detect uric acid.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a preparation method and application of a BSA-Au / Ag NCs / OPD / HRP proportional fluorescence sensor. Background technique [0002] Uric acid (2,6,8-trihydroxypurine) is the final product of purine catabolism in human nucleoproteins and nucleic acids. When the body has metabolic disorders, it will cause excessive purine, and the uric acid produced by purine metabolism will also increase accordingly. In general, excessive levels of uric acid can cause related diseases in humans, such as gout, kidney disease, and cardiovascular disease. Therefore, it is very necessary to analyze uric acid in the field of clinical diagnosis for early diagnosis of purine metabolism diseases. [0003] So far, there are many detection tools for uric acid in human serum, such as enzymatic method, uric acid sensor, electrochemical method, high performance liquid chromatography, etc. Compared with the prev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N21/64
CPCG01N21/64G01N33/5308
Inventor 孙见凡王晓艳刘振江胡文杰杜道林
Owner JIANGSU UNIV
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