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A kind of preparation method and application of fluorescent probe for detecting formaldehyde

A fluorescent probe, formaldehyde technology, applied in the field of fluorescent probes, can solve the problems of different application fields

Active Publication Date: 2019-04-02
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application fields of each detection method are different, and each has its own advantages and certain limitations.

Method used

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  • A kind of preparation method and application of fluorescent probe for detecting formaldehyde
  • A kind of preparation method and application of fluorescent probe for detecting formaldehyde
  • A kind of preparation method and application of fluorescent probe for detecting formaldehyde

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the synthesis of probe FAP:

[0024]

[0025] Dissolve 81.2 mg of potassium allyltrifluoroborate in 5 mL of ammonia methanol solution (concentration: 7 mol / L), and stir at room temperature for 15 min under nitrogen protection.

[0026] Dissolve 35.1 mg of raw material 1 in 2 mL of ammonia-methanol solution (concentration: 7 mol / L), add 20 μL of water, inject the reaction solution into ammonia-methanol solution of potassium allyltrifluoroborate, and react at room temperature for 16 h. After the reaction was completed, the solvent was removed by distillation under reduced pressure with a rotary evaporator to obtain a crude product. Using ethyl acetate and petroleum ether at a volume ratio of 1:4 as the eluent, the product was purified with a 200-300 mesh silica gel chromatography column to obtain 28.4 mg (70%) of a yellow solid.

Embodiment 2

[0027] Embodiment 2 probe changes with the addition of fluorescence spectrograms of different equivalents of formaldehyde

[0028] The probe FAP prepared in Example 1 was dissolved in DMSO to make a probe mother solution with a concentration of 1 mM, and a formaldehyde solution with a mass fraction of 37% was added to distilled water to prepare a formaldehyde mother solution with a formaldehyde concentration of 600 mM. Prepare acetonitrile:PBS buffer (0.01mM, pH=7.4) = 2:3 (volume ratio) spectrum solution, 3mL for each test tube. Take 60 μL (20 μM) from the probe mother solution and add it to a small test tube containing 3 mL spectrum solution, add different concentrations of formaldehyde (0, 0.25, 0.5, 2, 3, 4, 6, 7, 8, 10, 12, 13 ,14,15,16,17,18,20,25,30mM). Fluorescence spectrum changes of probes and different equivalents of formaldehyde reaction solutions were tested with a fluorescence spectrometer. Fluorescence spectrum changes as image 3 shown. image 3 It is the a...

Embodiment 3

[0029] Embodiment 3 in calf serum probe FAP changes with the fluorescence spectrogram of different equivalents of formaldehyde

[0030] Take the probe mother solution and formaldehyde mother solution prepared in Example 2, and prepare a spectral solution of calf serum:PBS buffer (0.01mM, pH=7.4)=2:98 (volume ratio), 3mL per test tube. Take 60μL (20μM) from the probe mother solution and add it to a small test tube containing 3mL spectrum solution, add different concentrations of formaldehyde (0, 0.25, 0.5, 0.75, 1, 1.5, 2, 3, 3.5, 4, 4.5, 5 , 6, 7, 8, 9, 10, 12, 15 mM). Fluorescence spectrum changes of probes and different equivalents of formaldehyde reaction solutions were tested with a fluorescence spectrometer. Fluorescence spectrum changes as Figure 4 shown. Figure 4 It is the variation of the fluorescence spectrogram of the probe FAP in calf serum in Example 3 with the addition of different equivalents of formaldehyde. Among them, the fluorescence peak at 462nm, from...

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Abstract

The invention discloses a preparation method and application of a fluorescent probe for detecting formaldehyde and belongs to the technical field of preparation of formaldehyde probes. The fluorescent probe is a good ratio-type fluorescent probe, suitable for detecting formaldehyde in an aqueous solution and a biological sample. The fluorescent probe according to the invention has a structural formula that is shown in the description. The invention also provides the preparation method of a compound shown by the formula FAP. The synthetic steps of the preparation method are simple, the preparation method only requires one-step reaction, and the yield is high.

Description

technical field [0001] The invention relates to a preparation method and application of a formaldehyde fluorescent probe, belonging to the technical field of fluorescent probes. Background technique [0002] Formaldehyde is a colorless gas with a strong pungent odor. Soluble in water, alcohol and ether. Formaldehyde is gaseous at room temperature, usually in the form of aqueous solution, and is a recognized carcinogen. Formaldehyde can be produced by various natural and human-made activities. Such as fuel combustion, industrial production, and microbial emissions. Formaldehyde is a protoplasmic poison that can bind to proteins. High concentrations of formaldehyde can cause damage to human eyes, nose, respiratory tract, skin and other easily exposed organs. Formaldehyde detection methods mainly include: spectrophotometry, electrochemical detection, gas chromatography, liquid chromatography, sensor method, etc. However, the application fields of each detection method are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D277/66C09K11/06G01N21/64
CPCC07D277/66C09K11/06C09K2211/1007C09K2211/1037G01N21/64G01N2021/6419
Inventor 郑开波周洋晏佳莹肖述章李德江
Owner CHINA THREE GORGES UNIV
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