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Preparation method of silver nanoparticle/sulfur quantum dot double-doped MOF compound ratio fluorescence exosome aptamer probe

A technology of silver nanoparticles and sulfur quantum, which is applied in the field of high-efficiency detection of tumor exosomes, can solve the problems of low cost and achieve the effect of simple preparation and novel design

Active Publication Date: 2020-11-03
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous literatures have reported the detection of exosomes, but these detection methods still have shortcomings, such as high sensitivity, specificity, reproducible quantitative detection of nanoscale tumor exosomes, low cost and high efficiency detection, etc.
At present, there are no domestic and foreign literature and patent reports based on nanomaterial double doping and fluorescence dual emission MOF nanocomposite probe AgNPs / Zn-SQDs / MOF for ratiometric fluorescence detection of exosomes

Method used

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  • Preparation method of silver nanoparticle/sulfur quantum dot double-doped MOF compound ratio fluorescence exosome aptamer probe

Examples

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Embodiment 1

[0017] The preparation method of the silver nanoparticle / sulfur quantum dot double-doped MOF composite ratio fluorescent exosome aptamer probe involved in this embodiment, the preparation schematic diagram of the aptamer probe is as follows figure 1 As shown, the specific preparation steps are as follows:

[0018]Add 1.5g of sublimed sulfur, 3mL of PEG-400 and 4g of NaOH to 75mL of double-distilled water under stirring to form a homogeneous mixture; Stir the reaction for 5 h; cool the product solution to room temperature, take 5 mL of the product solution, add 5 mL of 5 wt % zinc nitrate aqueous solution under stirring, and continue stirring for 30 min to obtain a Zn-SQDs aqueous dispersion.

[0019] Add 50mg of lipoic acid powder to 50mL of double distilled water under stirring, add 0.3mL of concentration to 1mol L -1 NaBH 4 Aqueous solution, stirred and reacted for 30min to form a homogeneous mixture; add 0.3mL to a concentration of 0.1mol L -1 Silver nitrate, then add 0....

Embodiment 2

[0023] The preparation method of the silver nanoparticle / sulfur quantum dot double-doped MOF composite ratio fluorescent exosome aptamer probe involved in this embodiment, the preparation schematic diagram of the aptamer probe is as follows figure 1 As shown, the preparation of Zn-SQDs and AgNPs aqueous dispersion is the same as in Example 1, and other specific preparation steps are as follows:

[0024] Add 1 mL of 5 mg mL to 10 mL of 2-methylimidazole in methanol under stirring -1 The AgNPs aqueous dispersion was stirred for 10 minutes to form a mixed solution; then 10 mL of zinc nitrate hexahydrate aqueous solution was added, stirred for 30 minutes to form a precipitate, washed three times with ethanol and distilled water, and centrifuged at 5000 rpm for 10 minutes to obtain the AgNPs / MOF composite aqueous dispersion solution; under stirring, add 1mL concentration of 6mg mL -1 The aqueous dispersion of Zn-SQDs was stirred and reacted for 2 hours; the product was centrifuged...

Embodiment 3

[0027] The preparation method of the silver nanoparticle / sulfur quantum dot double-doped MOF composite ratio fluorescent exosome aptamer probe involved in this embodiment, the preparation schematic diagram of the aptamer probe is as follows figure 1 As shown, the preparation of Zn-SQDs and AgNPs aqueous dispersion is the same as in Example 1, and other specific preparation steps are as follows:

[0028] Add 1 mL of 8 mg mL to 10 mL of 2-methylimidazole in methanol under stirring -1 The AgNPs aqueous dispersion was stirred for 10 minutes to form a mixed solution; then 10 mL of zinc nitrate hexahydrate aqueous solution was added, stirred for 30 minutes to form a precipitate, washed three times with ethanol and distilled water, and centrifuged at 5000 rpm for 10 minutes to obtain the AgNPs / MOF composite aqueous dispersion solution; under stirring, add 1mL concentration of 9mg mL -1 The aqueous dispersion of Zn-SQDs was stirred and reacted for 2 hours; the product was centrifuged...

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Abstract

The invention discloses a preparation method of a silver nanoparticle / sulfur quantum dot double-doped metal organic framework (MOF) compound ratio fluorescence exosome aptamer probe. The method comprises the following steps: reacting silver nanoparticles (AgNPs) with a precursor, doping AgNPs in an MOF structure, adsorbing zinc-doped sulfur quantum dots (Zn-SQDs) on the MOF surface, and attachingan aptamer / dopamine (Aptamer / DA) compound to form a nano composite aptamer probe; specifically combining the exosome (exosomes) and the aptamer to form an Aptamer / DA / exosomes compound; since the aptamer carries DA and is far away from the surface of the MOF, recovering Zn-SQDs blue fluorescence while AgNPs red fluorescence is almost unchanged; and fitting the linear relationship between the fluorescence peak intensity ratio IZn-SQDs / IAgNPs and the exosome concentration, and constructing a ratiometric fluorescence exosome aptamer probe.

Description

technical field [0001] The invention belongs to the technical field of preparation of metal-organic framework nanocomposites and ratiometric fluorescent exosome aptamer probes, in particular to ratiometric fluorescent exosomes based on silver nanoparticles and zinc-sulfur quantum dot double-doped metal-organic framework composites A method for preparing an aptamer probe, and the prepared probe is used for efficient detection of tumor exosomes. Background technique [0002] Metal-organic frameworks (MOFs) have many advantages, such as large specific surface area, high porosity, orderly lattice structure, good stability, etc., showing important application prospects in functional nanocarriers. MOF is combined with phosphors such as organic dye molecules, polymers, organic / inorganic hybrids, and nanomaterials to form a composite system in which phosphors are loaded on the inner cavity or outer surface of MOF, that is, phosphor-doped MOF composites, which have developed into Fl...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/58C09K11/56G01N21/64
CPCC09K11/06C09K11/58C09K11/565G01N21/6428G01N2021/6432G01N2021/6439C09K2211/188C09K2211/1044Y02A50/30
Inventor 桂日军姜晓文金辉
Owner QINGDAO UNIV
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