Application of quantum dot fluorescent probe to visual detection of Hg < 2 + > in cells and human blood

A fluorescent probe, quantum dot technology, applied in fluorescence/phosphorescence, nanotechnology for materials and surface science, nano-optics, etc. The effect of signal strength

Inactive Publication Date: 2020-06-19
杭州众道光电科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection methods of mercury ions are mainly divided into two categories, one is the detection method relying on large analytical instruments, mainly including atomic absorption spectrometry (AAS), atomic fluorescence spectrometry (AFS), inductively coupled plasma mass spectrometry (ICP). -MS), resonance Rayleigh scattering spectrometry (RSS), electrochemical method, gas chromatography, liquid chromatography, mass spectrometry, and a variety of instrumentation techniques, etc. Most of these methods require enrichment and separation of samples, etc. The professional and cumbersome pretreatment process has certain safety hazards for the operator, and the instrument is expensive to operate and maintain, and the detection cost is too high

Method used

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  • Application of quantum dot fluorescent probe to visual detection of Hg &lt; 2 + &gt; in cells and human blood
  • Application of quantum dot fluorescent probe to visual detection of Hg &lt; 2 + &gt; in cells and human blood
  • Application of quantum dot fluorescent probe to visual detection of Hg &lt; 2 + &gt; in cells and human blood

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Experimental program
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Embodiment 110

[0036] Example 110Ca / 6Tb:Bi 2 o 2 Preparation of S nanocrystals

[0037] (1) 0.84 mmol of bismuth acetate, 0.06 mmol of terbium acetylacetonate, 0.1 mmol of calcium acetate, 5 ml of oleic acid were added to a 50 ml three-necked flask at room temperature and the temperature was raised to 110° C. and kept for 1 hour;

[0038] (2) After the solution in step (1) is cooled to below 50°C, add 10 mmoles of sulfur powder and 20 ml of oleylamine to evacuate the three-necked bottle with a mechanical pump for about 10 minutes, then heat up to 120°C and keep it warm for 30 minutes, then in Rapidly raise the temperature to 310°C under the protection of argon and keep it warm for 1 hour;

[0039] (3) After the solution in step (2) was cooled to room temperature, ethanol was added to centrifuge to obtain a precipitate, and the product was washed with a mixture of ethanol:cyclohexane at a ratio of 3:1, and then dried at 40°C to obtain the final product.

[0040] Powder X-ray diffraction an...

Embodiment 2

[0050] Example 2 SiO 2 @10Ca / 6Tb:Bi 2 o 2 S@CDS Synthesis

[0051] 1) Synthesis of silica microspheres

[0052] First, 80.0 mL of ethanol, 4.850 mL of water, and 3.6 mL of 25% ammonia water were sequentially added to a 250.0 mL flask, and heated to 55° C. under vigorous stirring. Then quickly add a mixed solution of 3.1mL tetraethoxysilane (TEOS) and 8.0mL ethanol to the flask; keep the temperature of the solution at 55°C, and react for 5h to obtain silica with a particle size of about 40±5nm Microspheres, the prepared microspheres are used as seeds for the continued growth of the microspheres;

[0053] In a 250.0mL flask, add 10.0mL of the seed solution of silica microspheres prepared by the above reaction, 70.0mL of ethanol, 13.0mL of water and 7.5mL of 25% ammonia water, mix and stir, and add the prepared solution dropwise. A mixed solution of 1.0mL tetraethoxysilane and 10.0mL ethanol, kept stirring continuously for more than 5 hours, can produce silica microspheres w...

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Abstract

The invention belongs to the technical field of quantum dot fluorescent probe fluorescence detection, and especially relates to an application of a quantum dot fluorescent probe in visual detection ofHg < 2 + > in cells and human blood. The quantum dot fluorescent probe comprises silicon dioxide microspheres and 10Ca/6Tb: Bi2O2S nanocrystalline and aminated fluorescent carbon quantum dots, wherein the surface of the silicon dioxide microspheres is aminated and then is modified with a layer of 10Ca/6Tb: Bi2O2S nanocrystal, and the outer layer of the 10Ca/6Tb: Bi2O2S nanocrystal is further coated and modified with aminated fluorescent carbon quantum dots. The quantum dot fluorescent probe adopts SiO2 (at) 10Ca/6Tb: SiO2 (at) 10Ca/6Tb fluorescent probe, dual emission fluorescence can be realized, the quantum dot fluorescent probe can be used for a ratiometric fluorescence analysis method and does not depend on expensive instruments when being used for in-vitro analysis and can completelyperform quantitative or semi-quantitative analysis by naked eyes, can detect the fluorescence ratio intensity under two emission wavelengths or excitation wavelengths, can well correct errors causedby environment detection and improves the signal intensity.

Description

technical field [0001] The invention belongs to the technical field of fluorescent detection of quantum dot fluorescent probes, in particular to the use of quantum dot fluorescent probes in cells and human blood Hg 2+ Applications in Visual Inspection. Background technique [0002] Mercury is one of the most toxic metal elements, mainly Hg0, Hg 2+ And there are three forms of organic mercury, among which organic mercury is the most toxic. Hg 2+ After being absorbed, it can enter the whole body and various tissues and organs through the blood circulation system, which can cause abnormalities in the central nervous system and irreversible and permanent damage to internal organs such as liver and kidney, leading to symptoms such as loss of appetite and laziness. Detection of Hg using fluorescent probes 2+ With the characteristics of high sensitivity and high resolution, currently the main research probes include carbon quantum dots, MOF or organic systems. The optical prope...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/84B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/7746G01N21/6428G01N2021/6432
Inventor 陆阳清王凯陈泽楷张鑫洋
Owner 杭州众道光电科技有限公司
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