171 results about "Semiquantitative analysis" patented technology

Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and/or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and/or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA-tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.

The present invention provides a method and a device that utilizes capillarity-mediated, chromatographic transport, for the qualitative or semi-quantitative analysis of selected analytes in liquid samples. The device utilizes an applicator/collection device for collecting and administering the sample to the flow path such that reagent(s) flow through the applicator/collection device, washing the sample into the reaction pathway. The device farther utilizes an air gap between the initial location of the reagent and the reaction pathway to funnel the reagent efficiently through the sample so as to collect all or substantially all of the sample and make it available for the reaction(s).

The invention defines an all-solid-contact ISE which comprises a transducer layer of carbon nanotubes which brings the sensing layer and conducting element into contact. The invention also defines a method for the preparation of said all-solid-contact ISE and the use of the same for the qualitative, quantitative or semi-quantitative analysis of analytes. Said all-solid-contact ISE makes it possible to detect or quantify highly diverse chemical species in a reliable and reproducible manner, with the added advantages derived from its simplicity and low construction cost.

A nano colloidal golf linked immunoassary for detecting carbofuran includes such steps as coupling the immunized semi-antigen of carbofuran with BSA, synthesizing artificial immunoantigen compound, immunizing animal to prepare specific high-valence BFNH antibody, separating and purifying, linking the nano-class colloidal golf to said specific antibody, solidifying said linked compound, carbofurancoupled by egg albumin and sheep's mouse IgG antibody on a carrier, and for semi-quantitative analysis. Its advantage is high sensitivity and speed.

Systems and methods according to the present invention provide a means for determining, as an example, the health or location of nerves. A determination according to the present invention may include semi-quantitative threshold testing using intra-operative electrical stimulation to determine whether one or more nerves are functioning as expected. A determination according to the present invention may also be utilized to determine or predict the success of a surgical procedure that may affect the functioning of one or more nerves. Semi-quantitative analysis may be performed by comparing electrical stimulation intensity, or an approximation thereof, at which a threshold neural response is observed to an expected or previously applied electrical stimulation intensity, or an approximation thereof.

The invention discloses a method for realizing semi-quantitative analysis to trace mixture by use of the surface enhanced raman spectroscopy, and in particular relates to a qualitative identification method for carrying out semiquantitative analysis on a trace mixture by determinng Raman spectrum of a trace chemical substance based on surface enhanced Raman scattering effect and improving main component analysis. The method discosed by the invention adopts a lever law and a triangle parapllel line reading method to realize the semiquantitative analysis of the mixture components. The method can be used for analyzing mixture of entirely different chemical substances and can be used for analyzing mixture of homologous series. The method can realize semi-quantitative analysis to components of mixture based on the PCA (Principal Component Analysis) qualitative analysis and is a simple, rapid and accurate mixture identification method.

The invention discloses a raw material formulation for vanilla-seed fermented milk, which includes raw milk, lactalbumin powder, a milk-fat product, a sweet substance, a thickening agent, zymogen and vallina-seed jam with viscosity not being higher than 10cm/(20 DEG C, 60s); and the invention discloses a semiquantitative analysis method for addition of the jam, which comprises steps of 1) covering vanilla-seed fermented milk uniformly on an entire filter-paper surface; 2) drying filter-paper surface with the vanilla-seed fermented milk for 15 to 40 minutes; and 3) comparing with a series standard sample. The invention also discloses a preparation method of the vanilla-seed fermented milk, which comprises the steps of: 1, mixing the fermented milk and the vanilla-seed jam to be canned; and 2, implementing the analysis according to the semiquantitative analysis method and fetching the vanilla-seed fermented milk reaching the target addition of the jam. The invention also discloses the vanilla-seed fermented milk prepared through the preparation method. The vanilla-seed fermented milk combines the light fermented flavor of the fermented milk and the fragrance of the vanilla and has rich color, the addition of the jam can be semiquantitatively monitored, the quality of different product batches is stable, and the market potential is remarkable.

A method to detect volatile component in yellow wine belongs to technical field of yellow wine specialty analysis. The invention adopts coupled technique of roof solid phase extraction and gas phase chromatography-mass spectrum to make qualitative and semi-quantitative analysis of volatile component in yellow wine. The method can detect volatile compound in yellow wine quickly and estimate its proportion in all volatile compound initiatively. Roof solid phase extraction can complete under normal temperature without organic solvent, which avoid producing new compound during analyzing and enrich volatile component effectively. Mass spectrum detection can identify volatile component accurately to improve accuracy of the method. The invention has significance in researching micro constituent in yellow wine and improving its quality.

The invention discloses a determination method for filthy components of a high-voltage insulator. The determination method comprises the specific steps: A, pre-treating a sample; B, qualitatively and semi-quantitatively analyzing, namely determining varieties and corresponding contents of elements in the sample by using an X-ray photoelectron spectroscopy, and determining chemical group varieties in the sample by using a fourier transform infrared spectrometer; and C, quantitatively analyzing, namely carrying out an ICP (Inductively Coupled Plasma) test to determine the content of cations in the sample and carrying out an IC (Integrated Circuit) test to determine the content of anions in the sample. According to the determination method for the filthy components of the high-voltage insulator, the filthy components of the high-voltage insulator can be qualitatively and quantitatively analyzed by organically combining an X-ray photoelectron spectroscopy (XPS) test, a fourier transform infrared spectrometer (FTIR) test, the ICP test and an IC test result; the filthy components of the high-voltage insulator can be accurately determined so as to be good for preventing a pollution flashover accident.

The invention discloses a rapid detection method of synthetic pyrethroid pesticide residual, which comprises the following steps: (1) extraction the detected material: putting the synthetic pyrethroid pesticide residual in the reactor; (2) decomposition, gas production reaction: decomposing the detected material into compound within cyanide; adding the solid particle of sodium borohydride and adsorption host to mix with the detected material; adding sulphuric acid solution in the reactor to react large amount of gas; (3) colour reaction: guiding the decomposed gas in the absorber; reacting with the specific developer to make solution display blue; (4) qualitative analysis: generating qualitative or semiquanlitative analysis of synthetic pyrethroid pesticide in comparison with the colorimetric card.

The invention relates to a screening method of a volatile organic compound in a cigarette filter tip blasting-bead. The method comprises the steps of (1) performing headspace sampling on liquid in the blasting-bead, and combining a combined comprehensive two-dimensional gas chromatography mass spectrometry for carrying out full-scanning analysis on a sample to obtain a full-scanning spectroscopy; (2) automatically comparing chromatographic peaks of the full-scanning spectroscopy and database information through data software to obtain a preliminary qualitative result of each spot; (3) checking the preliminary qualitative result of each spot, combing a peak structure and similarity proportion, optimizing the full-scanning spectroscopy to obtain an optimal spectroscopy, and manually comparing with the database information to obtain an optimal qualitative result; (4) using percentage response for carrying out semi-quantitative analysis on the optimal qualitative result. The method has the advantages of short detection time, simplicity and convenience in operation, high sensitivity, comprehensiveness in screening, and the like, not only can be used for monitoring conventional harmful volatile organic compounds, but also can be used for monitoring if flavor components allowed to be added exceed the limitation or not so as to ensure the safety of blasting-bead products.

The invention discloses a method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables. With an ion mobility spectrometry technology as a basic detection technology, a quick qualitative and semi-quantitative analysis method for detecting pesticide residues based on an ion mobility spectrometry is established by a positive ion and negative ion mode. The method comprises the following steps: baking sampled sheets containing different pesticide samples for drying solvents, and then, conveying the sampled sheets into an ion mobility spectrometry thermal analysis sample injector to detect and analyze the sampled sheets to obtain detection signals, and performing qualitative and semi-quantitative analysis according to sample peak migration time and signal strength. Limit of detection of pesticide standard measurement can be less than 1ng. The measurement method is simple, convenient, quick, efficient and highly reliable, and can be widely used for analyzing pesticide residues on surfaces of fruits and vegetables in the food safety field.

A semi-quantitative analysis on the risk management process increases the possibility of performing an accurate risks comparison, making easier the identification of which risks shall be prioritized and shall receive the greatest mitigation efforts. Specifically, the semi-quantitative risk analysis enables an improved risks comparison for evaluating the consequences of each risk considering its impacts on the project's Net Present Value (NPV), reflecting the project's cash flow at different times. The use of such method makes the prioritization process more efficient, helping the managers and other personnel involved on the process to focus their efforts to the most critical risks for the project's success. In this sense, the risk management process becomes more efficient and better able to provide better support to the project decision makers.

The invention relates to a test kit for detecting tumor M2 pyruvate kinase and a relative preparation method, belonging to biological medicine and medical test technical field. The inventive test kit comprises a bottom plate (1), a colorful particle conjugate pad (3), a test sample pad (4), a water absorption pad (5) and a porous filter membrane for reaction, wherein the conjugate pad is fixed with anti-tumor M2 pyruvate kinase antibody marked by colorful particles, the porous filter membrane is arranged with a test region (a) and a reference region (b), the test region covers anti-tumor M2 pyruvate kinase antibody, the reference region covers anti-mouse IgG or antigen solution. The invention can process semi-quantitatively analyze and measure tumor M2 pyruvate kinase in 10min. The test kit is portable and easy to store, with simple operation, stable method, non additional tester and enzyme and the application for quickly and simply detecting tumor M2 pyruvate kinase on solid membrane medium.

The invention provides a novel color-developing agent luminosity analytical method for measuring hydrogen cyanide content in mainstream smoke quickly, which comprises the following steps of: pretreating a sample in which hydrogen cyanide in the mainstream smoke is collected, adding a color-developing agent, and quantifying by a luminosity analytical method, wherein the color-developing agent is 4-nitrobenzaldehyde and o-dinitrobenzene; preparing potassium cyanide (KCN) solution which is added with the color-developing agent and has different concentrations to make a standard curve; and quantifying the standard curve by an external standard method, adding the color-developing agent in a cigarette absorption liquid sample, and performing photometry detection at wavelength 533 nm by an ultraviolet spectrophotometer. In the method adopting the color-developing agent containing 4-nitrobenzaldehyde-o-dinitrobenzene, the selectivity and sensitivity are high, a reaction is completed instantaneously and the interference resistant capacity is high. In addition, the method is simple in operation, cigarette sidestream smoke can be detected, the HCN in smoke environment also can be analyzed qualitatively and semiquantitatively, and the method is also suitable for field detection.

The invention discloses a kit for detecting protein glycosylation subtype and a detection method thereof. The detection kit comprises agglutinin, confining liquid, a biotin labeled antibody, horse radish peroxidase labeled streptavidin, a 3',3',5',5'-tetramethyl benzidine substrate developing solution, a stop solution and a cleaning buffer solution. The detection method comprises the following steps: fixing the agglutinin onto a polystyrene solid phase carrier to be used for recognizing and binding glycoprotein in the sample based on specific recognition of agglutinin and an oligosaccharide chain, recognizing the glycoprotein needing to be detected by virtue of specific binding of the protein and the antibody thereof, and realizing semi-quantitative analysis of the glycoprotein. According to the detection kit and the detection method disclosed by the invention, semi-quantitative detection of single protein glycosylation subtype can be realized, and the kit is easy and convenient to operate and low in cost.

The disclosed molecular identification method and specific primer with upper/lower primer as SEQ ID No:1/2 for dominant flora structure in aquatic animal enteron comprise: 1) obtaining different fragments and contents of enteron as sample; 2)extracting sample total DNA; 3) with former DNA as template, taking PCR amplification; 4) taking denaturized gradient gel electrophoresis to target fragment, obtaining DGGE fingerprint spectrum by Ag dyeing, and setting another sequenching group; 5) taking cluster and semi-quantitative analysis; 6) recovering dominant strip as template for PCR amplification and sequenching; 7) comparing and analyzing the result to determine structure.

The invention relates to a COVID-19 (coronavirus disease 2019) nucleic acid testing method capable of increasing the accurate rate. firstly, a reversal reaction is performed in a reversal reaction system, so that RNA of polyA is subjected to reverse transcription under the assistance of reverse transcriptase and oligo (dT) to form cDNA of poly (T); meanwhile, cDNA subjected to reverse transcription is subjected to an RPA reaction for a period of time for further amplification; an amplified product is mixed with a QPCR (real-time quantitative PCR detecting system), and the mixture is subjectedto a chain reaction on a QPCR instrument and then sequentially subjected to denaturation, reannealing and final extended chain circular reaction respectively; fluorescence signal intensity is collected and detected through detection equipment, qualitative or semiquantitative analytic determination on target segments, and finally, the virus detection result of a patient is judged according to obtained different fluorescence signal data. According to the technical scheme, the COVID-19 nucleic acid testing method which is high in specificity, accurate is result, low in false negative probability,capable of monitoring each stage from the early period to the later period of infection of the patient and capable of increasing the accurate rate can be provided.

The invention discloses a semi-quantitative solid-phase reaction detection technology for content of an object to be detected, a preparation method and application. The technology is a semi-quantitative colloidal metal immune chromatographic detection technology capable of directly detecting the content of the object to be detected, in which the sample of the object to be detected is reacted with a standard comparison product of the object to be detected with known concentration under the same reaction system, wherein a solid-phase membrane, a supporting sheet, a developing agent, a sample applicator and the standard comparison product are provided in the technology; various ready-to-use products for detecting the content of the object to be detected can be prepared; and the technology can be effectively used for clinical detection and detection on the content of the object to be detected, such as foods, medicaments, laboratory supplies and the like, and has high application value and market prospect.

The invention discloses a large-scale blood plasma sphingolipid profile analysis method based on liquid chromatography-mass spectrometry combination, which is characterized in that sphingolipid in blood plasma is extracted by double-phase extraction with methyl tertiary butyl ether/methanol/water (MTBE/MeOH/H2O) combined with mild basic hydrolysis. Then rapid semi-quantitative analysis is realized within 15 min by combination of ultra-high performance liquid chromatography-electro-spray ionization-mass spectrometry. In the invention, the MTBE/MeOH/H2O extraction system is simple, rapid, and easy to operate, has less protein interference in the extract, and has good repeatability; interference of high-abundance phospholipid and glyceride in the lipid group is eliminated by hydrolysis; ion inhibition is reduced; therefore, the purpose of detecting low-abundance sphingolipid with high sensitivity is achieved. With the high resolution separation capability of subsequent ultra-high performance liquid chromatography (UHPLC) and the specificity of MRM, effective distinguishing of isomers is realized, and the introduction of complex isotope correction during quantification is avoided, which makes the method more rapid and accurate.

The invention discloses a test paper based on a photosensitive meso-porous silicon-based molecular imprinting microsphere and a preparation method thereof. The method comprises the following steps: by taking a mesoporous SiO2 nanosphere with the surface modified by double bond as a carrier, a to-be-detected object as a template molecule, azobenzene compound as a functional monomer, dimethyl acrylate as a cross-linking agent, azo isobutyronitrile as an initiator and acetonitrile as a pore-foaming agent, preparing the photosensitive meso-porous silicon-based molecular imprinting microsphere, and then removing the template molecule with a solvent under a light-operated effect, thereby acquiring the molecular imprinting microsphere. According to the technology, the thickness of the polymer shell of the nuclear shell type molecular imprinting microsphere can be strictly controlled, the regulation optimization of the adsorption capacity can be realized, the binding strength of the polymer shell and the mesoporous silicon core can be enhanced and the mechanical damage of the subsequent process to the molecular imprinting microsphere can be avoided. The test paper prepared according to the invention utilizes the photosensitive isomerization and photochromic principle of the functional monomer of the polymer to realize the photosensitive recognition of the molecular imprinting microsphere for the detected object and semi-quantitative analysis can be performed according to the color depth.

The invention discloses a method for measuring aroma components in main stream smoke of cigarettes. The method comprises the following steps: (1) placing a filter disc gathered with total particular matters of the main stream smoke of the cigarettes in an extracting solution and extracting, and filtering to obtain filtrate which is a sample solution, wherein the extracting solution is any one of acetone, dichloromethane and hexamethylene; (2) analyzing the sample solution obtained in the step (1) with a comprehensive two-dimensional gas chromatography-gas chromatograph-mass spectrometer; and (3) qualitatively and semi-quantitatively analyzing smoke components by using GC Image software. By the method, a small amount of cigarettes can be directly collected by using a smoking machine standard method to prepare the sample solution, and a complicated sample pretreatment process is not required. The method has the advantages of quick and efficient analysis, small interference, high resolution ratio, large peak capacity, accurate identification and the like, is simple to operate, and can identify various aroma components. Problems of limited peak capacity, insufficient resolution ratio, severe peak overlapping and the like in the traditional chromatography are avoided, and reliability of mass spectrometric identification is improved.