Method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables

A technology for pesticide residues, fruits and vegetables, which is applied to the instrument for sensitive detection of pesticide residues on the surface of fruits and vegetables, in the fast field, can solve the problems of limited application scope of enzyme-linked immunosorbent assay, difficulty in antibody preparation, and dependence on foreign imports of antibodies, etc. Simple measurement method and cost-effective effect

Inactive Publication Date: 2014-03-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

Due to the wide variety of pesticides, the difficulty of antibody preparation (about 50 kinds), the blindness of the detection when it is not certain that there are pesticide residues in the sample, and the dependence of antibodies on foreign imports, the application range of ELISA is limited. big limit

Method used

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  • Method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables
  • Method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables
  • Method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take 1-50μL, 0.5-100ppm chlorothalonil pesticide sample solution with a micro-sampler. Using the ion mobility spectrometer as the detection instrument, the sample sheet (containing 100ng chlorothalonil sample) is evaporated by the heater (50°C) to remove the solvent, and the carrier gas carries the pesticide sample and enters the ion mobility spectrometer for analysis and detection; The region is ionized into positive and negative ions, which enter the drift region formed by a uniform electric field through the periodically opened ion gate, and are separated and detected in the drift region. Different compounds have different mobility constants, move at different speeds in the drift region, and take different times to reach the detector. By measuring the time of ion arrival at the detector and the peak intensity, the compound can be identified and its amount is measured based on the signal intensity of the peak.

[0051] The detection signal was obtained in the negativ...

Embodiment 2

[0054] Gently wipe the surface of the pear without chlorothalonil pesticide with the sampling sheet (three times) (accurately calculate the size of the sampling area, the sampling area is 3cm 2 ), get as Figure 6 The detected signal is shown. There is a signal of about 15mv around about 9.3ms. Coat the pear surface with 100ppm chlorothalonil pesticide, and then use the sampling slice to sample the pear surface (accurately calculate the size of the sampling area, the sampling area is 3cm 2 ), the detection signal is about 80mv (such as Figure 7 ). If the sampling amount detects that the signal exceeds 15mv, it can be detected.

Embodiment 3

[0056] For the experiment of detection limit of pesticides, the experiment detects 1ng chlorothalonil pesticide standard. Under the experimental conditions, a signal between 20-50mv can be obtained, and the detection limit should be at least 1ng calculated according to the signal-to-noise ratio of 3 times.

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Abstract

The invention discloses a method for quickly and sensitively detecting pesticide residues on surfaces of fruits and vegetables. With an ion mobility spectrometry technology as a basic detection technology, a quick qualitative and semi-quantitative analysis method for detecting pesticide residues based on an ion mobility spectrometry is established by a positive ion and negative ion mode. The method comprises the following steps: baking sampled sheets containing different pesticide samples for drying solvents, and then, conveying the sampled sheets into an ion mobility spectrometry thermal analysis sample injector to detect and analyze the sampled sheets to obtain detection signals, and performing qualitative and semi-quantitative analysis according to sample peak migration time and signal strength. Limit of detection of pesticide standard measurement can be less than 1ng. The measurement method is simple, convenient, quick, efficient and highly reliable, and can be widely used for analyzing pesticide residues on surfaces of fruits and vegetables in the food safety field.

Description

technical field [0001] The invention relates to a method for detecting dozens of pesticide residues on the surface of fruits and vegetables, in particular to a new instrument and method for quickly and sensitively detecting pesticide residues on the surface of fruits and vegetables. The method is based on ion mobility spectrometry technology, using ion mobility spectrometry as an analysis method, the detection limit of pesticides can reach below 1ng. The measuring method is simple, fast, efficient and reliable. The method has fast analysis speed, simple test operation, and does not require personnel with special professional background to operate. Background technique [0002] With the continuous improvement of cultivation technology, the growth period of vegetables has become shorter and shorter, and with the aggravation of environmental pollution, the pests and diseases of vegetables have become more and more serious. Leafy vegetables with heavy pesticide pollution, amon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/622
Inventor 王新李京华侯可勇王卫国李杨李海洋
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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