Micro-fluidic chip and manufacturing method thereof

A microfluidic chip and microfluidic technology, applied in the direction of chemical instruments and methods, measuring devices, instruments, etc., can solve the problems of large sample consumption and complicated operation steps, and achieve short reaction time, strong anti-interference, and detection Easy to operate and masterable effects

Inactive Publication Date: 2012-08-01
BEIJING JINZHIJIE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defects of large amount of samples and complicated operation steps in the biological detection process in the prior art, the present invention provides a microfluidic chip capable of detecting a variety of different samples at the same time and its preparation method. The microfluidic chip provided by the present invention The flow control chip can detect many different substances in multiple samples at the same time. The detection method provided by the present invention is simple to operate, requires less reagent, saves time, and is highly efficient and accurate.

Method used

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  • Micro-fluidic chip and manufacturing method thereof
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  • Micro-fluidic chip and manufacturing method thereof

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Effect test

Embodiment 1

[0083] Such as figure 1 , image 3 As shown, the present invention provides a microfluidic chip that can be used for detection. The microfluidic chip includes a base layer 1, a functional layer and a microfluidic pipeline layer 2 from bottom to top; the functional layer is fixed on the The upper surface of the base layer 1, the functional layer is composed of six parallel functional strips 3, the functional strips are strips formed of materials that can react with specific substances, and the functional strips are coated Antibody; the microfluidic pipeline layer 2 is covered on the functional strip 3, and the lower surface of the microfluidic pipeline layer 2 is provided with seven pipelines 4, and the pipeline 4 is connected to the functional strip 3 Pass.

[0084] The base layer 1 is a polydimethylsiloxane (PDMS) film with a thickness of 2mm. Seven parallel cuboid pipelines 4 are distributed on the bottom of the microfluidic pipeline layer 2. The cuboid pipeline 4 is 0.5mm...

Embodiment 2

[0109] Such as Figure 1 to Figure 8 As shown, the present invention provides a microfluidic chip that can be used for detection. The microfluidic chip includes a base layer 1, a functional layer and a microfluidic pipeline layer 2 from bottom to top; the functional layer is fixed on the The upper surface of the base layer 1, the functional layer is composed of six parallel functional strips 3, the functional strips are strips formed of materials that can react with specific substances; the microfluidic pipeline layer 2 Covering the functional strip 3 , seven channels 4 are arranged on the lower surface of the microfluidic channel layer 2 . The pipeline 4 communicates with the functional strip 3 .

[0110] The base layer 1 is a polydimethylsiloxane (PDMS) film with a thickness of 2mm. Seven parallel cuboid pipelines 4 are distributed on the bottom of the microfluidic pipeline layer 2. The cuboid pipeline 4 is 0.5mm wide, 0.7mm high, and 40mm long. , interval 2mm between pipe...

Embodiment 3

[0121] Such as Figure 3 to Figure 8 As shown, the present invention provides a microfluidic chip, including a base layer 1, a functional layer, and a microfluidic pipeline layer 2; the functional layer is fixed on the upper surface of the base layer 1, and the functional layer includes six Parallel functional strips 3; the microfluidic pipeline layer 2 is provided with five pipelines 4 (such as image 3 As shown), the pipes 4 communicate with the functional strips 3, and the two ends of each pipe 4 are provided with through holes 5 communicating with the atmosphere.

[0122] The above base layer is made of polydimethylsiloxane film.

[0123] The thickness of the above-mentioned base layer is 0.5mm, and the bottom of the microfluidic pipe layer is distributed with cuboid pipes. The width of the cuboid pipes is 0.01mm, the height is 0.01mm, and the length is 10mm. The distance between the pipes is 1mm. 1mm, the interval between the injection holes on the same side of the adja...

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Abstract

The invention relates to the technical fields of biological detection, environment monitoring and clinical detection, in particular to a micro-fluidic chip and a manufacturing method thereof, which are used for overcoming the defects of large sample using amount and complex operating steps existing in the biological detection process in the prior art. The invention provides a micro-fluidic chip and a manufacturing method thereof. The micro-fluidic chip comprises a substrate layer, a functional layer and a micro-fluidic pipeline layer, wherein the functional layer is fixed on the substrate layer, and comprises at least two parallel functional strips; the functional strips are strip-shaped bands formed by a material which can react with a specific material; the micro-fluidic pipeline layer is provided with at least two pipelines; the pipelines are communicated with at least one functional strip; and the two ends of each pipeline are provided with through holes which are communicated with the atmospheric air. According to the micro-fluidic chip provided by the invention, various different substances in a plurality of samples can be detected simultaneously. A detection method provided by the invention has the advantages of easiness for operating, small reagent using amount, saving in time, high efficiency and accuracy.

Description

technical field [0001] The invention relates to the technical fields of biological detection, environmental monitoring and clinical detection, in particular to a microfluidic chip and a preparation method thereof. Background technique [0002] There are many methods for biological detection in the prior art, such as immunoassay methods such as immunoblotting and enzyme-linked immunoassay (ELISA). However, these immunoassay methods require relatively long analysis time, and the liquid processing process is also cumbersome, the throughput is relatively small (only one item can be detected at a time), and more biologically active reagents are required, while the microfluidic analysis chip can effectively overcome these shortcomings. [0003] Microfluidic immunoassay method is a newly developed technology in recent years. This method is mainly based on analytical chemistry, combined with the achievements of biochemistry, physical chemistry, immunology and other related discipli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/544
Inventor 李东霞刘俊罗次汐潘竹青沈海莹
Owner BEIJING JINZHIJIE BIOTECH
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