2082 results about "Micro fluidic" patented technology

A system for generating and detecting transient vapor nanobubbles in a fluid from a patient can include a micro-fluidic device to receive a flow of the fluid from the patient, a laser pulse source to provide a laser pulse to the flow of the fluid from a first side of the micro-fluidic device, a probe light source to provide a probe light to the flow from the first side of the micro-fluidic device, and a photodetector located at a second side of the micro-fluidic device opposite the first side. The photodetector can detect scattered, reflected, and / or deflected probe light and output a nanobubble signal including characteristics of optical scattering, reflecting, and / or deflecting by the transient vapor nanobubble.

The invention discloses an intelligent defect detection system and a self-repairing method for a flexible base material RFID tag, and belongs to the technical field of Internet of Things electronic device manufacturing. According to the system, a three-dimensional dynamic scanning system is constructed by integrating a high-resolution image acquisition module, a multispectral sensor array and a mechanical arm motion platform, and surface and internal structure characteristics of a flexible substrate are captured in real time. A defect identification algorithm based on deep learning is combined with a multi-scale convolutional neural network and a transfer learning technology, precise classification of 12 types of defects such as microcracks, conductive layer fractures and base material deformation is realized, and the detection precision reaches 99.2%. A dual-mode self-repairing mechanism is put forward, specifically, nano-silver conductive colloid is injected through a microfluid channel for the defects of the conductive layer, and 3D structure reconstruction is achieved through a controllable temperature field; for substrate damage, a photoresponse shape memory polymer patch is adopted, and molecular-level bonding repair is achieved after ultraviolet light activation. According to the scheme, the detection efficiency is improved by more than 5 times, and the radio frequency performance of the tag is recovered to 98.7% of the initial value after self-repairing.

A processor includes a first die, a second die connected to the first die with a microfluidic volume positioned between the first die and the second die, a wicking heat spreader positioned in the microfluidic volume; and a boiling enhancement surface feature positioned on at least one surface of the wicking heat spreader.

The invention belongs to the technical field of wearable sensing, and discloses a wearable patch for detecting glucose in sweat as well as a preparation method and application of the wearable patch. The wearable patch comprises a bonding layer, a microfluid channel, an electrochemical glucose biosensor, a temperature sensor and an electrothermal film which are sequentially arranged from inside to outside. The wearable patch is made of a flexible material, is resistant to high temperature and can be well attached to the skin, and meanwhile, the electrothermal film can be repeatedly used and is low in cost and easy to operate. Novel glucose detection equipment prepared based on the wearable patch can monitor glucose in sweat in a non-invasive mode, skin sweats in a thermal stimulation mode, a temperature control module is additionally arranged to serve as a protection mechanism, pain caused by a traditional blood sampling mode is avoided, glucose in sweat can be detected in situ in a resting state, and the detection accuracy is improved. The system is an ideal wearable real-time glucose monitoring platform, and has a wide application prospect in the aspect of early prevention of diabetes mellitus.

According to the reconfigurable flexible pressure sensor based on the magnetic ionic liquid drops and the preparation method of the reconfigurable flexible pressure sensor, the superparamagnetic nanoparticles and the ionic liquid drops are combined to prepare the magnetic ionic liquid drops which are high in fluidity and can be magnetically controlled, and the magnetic ionic liquid drops serve as a pressure sensitive layer of a pressure sensing device; and the flexible stretchable pressure sensor based on the magnetic ionic liquid drop array is constructed. The invention also provides a distributed / combined non-contact multi-element in-situ reversible reconstruction method of the flexible pressure sensor, and an external magnetic field is utilized to control the magnetic ion liquid drops in the sensor to carry out programmable low-resistance rapid movement and deformation between the super-hydrophobic interconnected microfluid channel and the chamber array. The spatial distribution, component / volume and geometrical shape changes of the magnetic ionic liquid drops in the array are regulated and controlled, and distributed / combined non-contact multi-element in-situ reversible reconstruction of the flexible stretchable pressure sensor in the performances of spatial resolution, sensitivity, detection limit, pressure response mode and the like is realized through one or more change combinations.

The invention relates to the field of microfluid chemical synthesis, and discloses a method for preparing gold nanoparticles based on two-phase flow microfluid control. The method comprises the following steps: 1) converging a gold precursor water phase, a reducing agent water phase and an organic phase in a micro-fluidic chip at 0-25 DEG C to form water-in-oil liquid drops; (2) conveying the fluid to a mixing area channel of a micro-fluidic chip, and carrying out instantaneous nucleation reaction to form a gold nanoparticle prototype; 3) conveying the fluid into a capillary reactor, and further nucleating, growing and curing the gold nanoparticle prototype; and (4) the fluid is subjected to phase splitting, and a gold nanoparticle solution is obtained. A three-in-one micro-fluidic chip is adopted, process parameters are optimized, and a gold precursor and a reducing agent are wrapped in an organic phase before reaction to form water-in-oil liquid drops, so that on one hand, the situation of adsorption and scaling of the inner wall of a channel can be avoided, and on the other hand, the heat and mass transfer efficiency of a material can be improved through the generated liquid drops; and the uniformity of a reaction nucleation environment is ensured.

A method and system to make a modular microfluidic device in a miniaturized form for cell culture, co-culture, and organ-on-a-microfluidic device as in-vitro model of human organs within compartmentalized 3D structures is described. The modular microfluidic device enables co-culturing of heterogeneous cell assemblies that are adjacent or sequentially connected to promote enhanced cell-cell interaction. In vivo-like flow into and / or from one cell growth chamber to adjacent cell growth chamber is maintained by micro-engineered porous barriers i.e., porous walls and membranes. These porous barriers provide a tool for mimicking the paracrine exchange between cells in the human body. The system comprises of a set of microelectrodes for real-time and long-term monitoring and quantitative measurement of the impact of stimuli on the epithelial tissue permeability. The modular microfluidic system provides a platform technology for bio-fabrication of variety of miniaturized in-vitro models for mimicking the structure and function of single and multi-organ models.

The invention discloses a coating die head uniform coating distribution method based on a microfluid optimization technology, which comprises the following steps: S1, analyzing the rheological characteristics and coating requirements of a coating, and designing a flexible microfluid channel structure; s2, optimizing the geometric design of the channel by adopting a lattice Boltzmann method simulation technology; s3, the interaction between the coating and the die head surface material is simulated and analyzed through molecular dynamics, and adaptive data is generated; s4, based on the optimized geometric design and the adaptive data, an intelligent dynamic adjustment module is embedded, and a dynamic adjustment mechanism is constructed; s5, synchronous and uniform coating of multiple layers of coatings is achieved through a double-layer micro-channel structure; s6, the thickness and defects of the coating are monitored in real time by combining optical and pressure sensors, and a repair path is generated by combining a parrot optimization algorithm; and S7, an active heat management module is embedded, and the heating power is adjusted to maintain the optimal flowing state of the coating. According to the method, microfluid optimization and intelligent optimization methods are combined, and coating uniformity improvement, defect real-time repair and multi-layer synchronous coating are achieved.

The invention discloses a control system and method for multi-channel treatment of a biological sample. A capture antibody layer of an optical fiber SPR sensor and a real-time detection biological solution can be prepared; comprising a microfluidic module, a sensing module and a computer, the sensing module is used for monitoring resonance wavelength information of a biological solution flowing through a sensing area of the optical fiber SPR sensor in real time; the micro-fluidic module is used for enabling the solution in the corresponding liquid storage tank to flow through the outer surface of the optical fiber SPR sensor in the micro-fluidic chip through the micro-fluidic channel under the set pressure; and the computer is used for detecting the corresponding content of the biological solution in the current liquid storage tank by measuring the resonant wavelength and comparing the resonant wavelength with pre-stored data based on the received electric signal of the spectrum. According to the control system, a micro-fluidic chip is used as a container for accommodating a sample to be detected or prepared, and a biological solution slowly flows into the micro-fluidic chip through a micro-fluid channel so as to realize preparation of a capture antibody layer or real-time detection of the biological solution.

A microfluidic device for analysing a test liquid comprises: a sensor provided in a sensing chamber; a sensing chamber inlet channel and a sensing chamber outlet channel, each connecting to the sensing chamber for respectively passing liquid into and out of the sensing chamber, and a reservoir forming a sample input port to the microfluidic device, the reservoir being in fluid communication with the sensing chamber inlet channel; a liquid collection channel; a barrier between an end of the sensing chamber outlet channel and the liquid collection channel; a first seal, covering the sample input port; a second seal, covering the end of the sensing chamber outlet channel, thereby preventing liquid from flowing from the sensing chamber, over the barrier, into the liquid collection channel; wherein the microfluidic device is filled with a liquid from the first seal at the sample input port to the second seal at the end of the sensing chamber outlet channel, such that the sensor is covered by liquid and unexposed to a gas or gas / liquid interface; and wherein the first and second seals are removable to cause the liquid to flow between the reservoir and the end of the sensing chamber outlet and over the barrier.

Aspects of the present invention relate to a pancreatic cancer organoids on a chip device including a microfluidic chip having a top and bottom surface and a thickness therebetween, a central chamber embedded in the microfluidic chip, at least one opening in the top surface of the chip fluidly connected to the central chamber with one or more channels, and a plurality of evenly spaced micropillars arranged in a substantially circular shape within the central chamber such that the central chamber is partitioned into at least a first inner region and a first outer region, wherein the first inner region includes an immunocompetent pancreatic region configured to mimic a pancreatic niche, and the first outer region includes a vascular region configured to mimic an adjacent vascular network.

Provided are photocurable resins comprising a poly(siloxane)-based copolymer together with a photoinitiator, and other optional ingredients such as a photocurable diluent, a photoabsorber, a photosensitizer, or a hydrophillic additive. Also provided are methods of stereolithographically printing a 3-D object from a disclosed resin. Also provided is an improved method for stereolithographically printing a 3-D object, the improvement comprising the use of a disclosed photocurable resin. Further provided is a 3-D microfluidic device such as an artificial lung prepared from a disclosed photocurable resin.

Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.

The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.

The invention discloses a high-precision liquid phase chip sample positioning and identification system, which belongs to the field of high-precision liquid phase chip sample positioning and identification, and comprises an active liquid phase sample positioning control module used for collecting a liquid flow state signal and adjusting the speed and position of an input sample in real time through an adjustable microfluid structure, fixed-point residence and directional conveying of samples in a chip channel are realized; the image recognition and feature position fine adjustment module is used for carrying out multi-modal image collection on an area where the sample is located, extracting spatial feature information of the target sample based on a deep learning recognition model, and carrying out fine adjustment correction on the position of the sample according to a feedback signal; by constructing an active liquid phase sample positioning and recognition system, high-precision directional control and spatial residence of a liquid sample in a chip channel are realized, the problems of sample drift and unstable recognition in a traditional passive conveying mode are effectively avoided, and the timeliness and accuracy of sample recognition are improved.

The invention relates to a design method and system for a monolithic integrated microfluid cooling channel of a power device, and the method comprises the steps: obtaining the size structure data, hot spot density data and heat source data of the power device, and carrying out the analysis of the microfluid cooling requirements, and obtaining the channel design requirements and heat source distribution information; performing microfluid channel network construction and surface insulation sealing treatment on a preset wide bandgap semiconductor substrate according to the channel design requirements to obtain a microfluid three-dimensional channel structure; performing power device active region layout according to the microfluid three-dimensional channel structure to obtain a device integrated structure; performing cooling simulation evaluation on the device integration structure according to the heat source distribution information to obtain a cooling evaluation result; and performing iterative verification optimization on the device integration structure according to the cooling evaluation result to obtain a target design scheme. The heat dissipation performance of the power device can be effectively improved, and the size of an external heat dissipation system is reduced.

A microfluidic device, a microfluidic system comprising said microfluidic device, and to a method of culturing biological material.

Payment cards with activated information displays are described. In one embodiment, a payment card may include: a first layer; a second layer comprising an elastic material; an actuator; and an information layer between the first layer and the second layer, comprising: a plurality of information voids; and a microfluidic channel that communicates the plurality of information voids with the actuator. A fluid may be provided in the microfluidic channel such that when the actuator is depressed, fluid flows into the information voids making the information voids visible through the second layer, and when the actuator is released, the fluid flows out of the information voids making the information voids substantially invisible through the second layer.

A device for reversibly blocking activities of a target region of a subject includes a microfluidic system configured to route a fluid around the target region to change a local temperature of the target region; and an electronic system coupled with the microfluidic system for providing a real-time feedback.

The invention discloses an inertial micro-fluidic chip integrating cell focusing and sorting and a preparation method thereof. The inertial micro-fluidic chip comprises a cover plate layer, a runner layer and a substrate layer, a chip inlet, a first chip outlet and a second chip outlet are formed in the cover plate layer; the flow channel layer is provided with a sample liquid flow channel inlet, a first flow channel outlet and a second flow channel outlet, the chip inlet is communicated with the sample liquid flow channel inlet, the first chip outlet is communicated with the first flow channel outlet, and the second chip outlet is communicated with the second flow channel outlet. The flow channel layer is provided with a spiral cell sorting flow channel and a cell focusing flow channel, the front end of the spiral cell sorting flow channel is communicated with a sample liquid flow channel inlet, the tail end of the spiral cell sorting flow channel is communicated with the cell focusing flow channel and a first flow channel outlet, and the tail end of the cell focusing flow channel is communicated with a second flow channel outlet. Through a microfluid vortex focusing technology and a spiral flow channel inertia sorting technology, accurate sorting and focusing of cells are realized, and the problems that the focusing state of the sorted cells is influenced and the cell counting detection precision is low are effectively solved.

The invention discloses a sweat uric acid and glucose sensor based on a carbon nanotube (CNT) enhanced electrochemical platform, and aims to solve the sensitivity challenge of low-concentration biomarker detection in sweat. The enzyme is covalently immobilized on the CNT through an EDC / NHS chemical method, so that the catalytic efficiency, the electron transfer speed and the sensor stability are remarkably improved, and the ultralow detection lower limit of uric acid and glucose is realized. According to the device, uric acid, glucose and pH sensing functions are integrated on a single wearable platform, efficient flowing and rapid updating of sweat are ensured through a radiation symmetry microfluid design optimized through finite element analysis, and real-time biomarker monitoring is supported. The material innovation (such as CNT-enzyme complex) and fluid dynamics optimization of the invention overcome the key problem in the field of wearable biosensing, and provide an extensible solution for the next generation of non-invasive health monitoring.

An electrowetting system is disclosed. The system includes electrodes configured to manipulate droplets of fluid in a microfluidic space. Each electrode is coupled to circuitry operative to selectively apply a driving voltage to the electrode. The system includes a dielectric stack including a first dielectric pair comprising a first layer having a first dielectric constant and a second layer having a second dielectric constant. The second dielectric constant is larger than the first dielectric constant. The dielectric stack includes a second dielectric pair comprising a third layer having a third dielectric constant and a fourth layer having a fourth dielectric constant. The fourth dielectric constant is larger than the third dielectric constant. A ratio of a thickness of the fourth layer to a thickness of the third layer (T4:T3) is in the range from about 2:1 to about 8:1. The second dielectric pair is thinner than the first dielectric pair.

The utility model relates to the technical field of cell cultivators, in particular to a pump-free perfusion micro-fluidic chip for multi-cell three-dimensional co-culture, which comprises a liquid storage layer, a liquid inlet and outlet layer, an upper channel layer, a lower channel layer and a packaging layer, realizes a one-way circulation perfusion dynamic co-culture system without a pumping system, reduces the occupied space of equipment, and is convenient to operate. Operation is simple, culture medium consumption is reduced, and cost is reduced; the separation of free NK cells and tumor infiltration NK cells is realized, and conditions are provided for researching molecular biological characteristics of different types of NK cells. The migration and interaction of the tumor spheres and the NK cells are observed in real time at the same time, so that the problem that a microfluid pump integrated by an external system is needed for separating free immune cells and tumor infiltration immune cells by existing equipment is solved.

Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).

The invention discloses a DLD chip and method based on high-flow-speed composite flow channel multi-target sorting. The chip comprises a sample inlet, a sheath fluid inlet, a rectification module and a two-stage deterministic lateral displacement DLD sorting module, the rectification module adopts a circular microcolumn array to optimize fluid distribution, and the first-stage module realizes primary sorting through a microfluid channel and is connected with the second-stage module; and the second-stage module is communicated with three sample outlets after being accurately sorted through a microfluid channel II. The chip is made of a PDMS material through a soft photoetching process, and the two stages of sorting modules are designed to be of a rectangular structure connected end to end and are respectively composed of elliptical micro-columns with opposite deflection angles. Under the high-flow-rate working condition, stable separation of cells can be kept, blockage is not prone to occurring, the good sorting effect on various cells of different sizes is achieved, the flow channel layout is compact, the chip manufacturing process is simplified, and the chip is suitable for the sorting scene with the high-flux requirement.

The invention belongs to the technical field of construction of super-hydrophobic surfaces, and particularly relates to a preparation method of a super-hydrophobic coating with a multistage micro-nano structure. Comprising the following steps: pretreating a substrate; slowly adding the F-SiO2 nanoparticle dispersion liquid into the fluorine-containing glue solution under the synergistic treatment of ultrasonic and magnetic stirring to obtain an F-SiO2 / fluorine-containing glue composite solution; the method comprises the following steps: determining the actual glue coating amount based on the theoretical glue demand amount of a PDMS template, and coating the F-SiO2 / fluorine-containing glue composite solution on the surface of a substrate through spin coating to form a wet film; coining the wet film by using a PDMS template, and stripping the template after ultraviolet curing to obtain the super-hydrophobic coating with the multistage micro-nano structure. Based on an imprint method with insufficient glue amount, synchronous construction of a micro / nano dual-scale structure is achieved by accurately controlling the ratio of the volume of the coating liquid to the volume of the pore of the template and combining the curing shrinkage characteristic of the polymer, and the method has application potential in the fields of anti-icing, microfluid devices, marine antifouling and the like.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

Microfluidic chips and microfluidic systems containing the same for implementing microfluidic oscillatory asymmetrical traps are described herein. In one example, an oscillatory microfluidic system for trapping particles contained in a fluid flowing within a channel is provided. The oscillatory microfluidic system including: the channel to conduct the fluid, where the channel includes a longitudinal axis and one or more geometric features that provide at least one asymmetry along the longitudinal axis; a source of pressure or flow to move the fluid through the channel; a fluid oscillator to provide an oscillating pressure on the fluid within the channel; and a controller arranged and configured to transmit control signals to the fluid oscillator to generate a repeating oscillation flow profile of the fluid within the channel, thereby trapping the particles in one or more zones centered on the one or more geometric features within the channel.

Some methods of performing an assay comprise culturing target cells in one or more channels of one or more microfluidics chips, where each of the channel(s) can have a volume that is less than or equal to 100 microliters (μL). For each of one or more test liquids that each comprise a therapeutic reagent, the test liquid can flow over the cultured target cells in at least one of the channel(s) and, while the test liquid flows over the cultured target cells, data indicative of an interaction between the target cells over which the test liquid flows and the therapeutic reagent of the test liquid can be captured.