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2008 results about "Micro fluidic" patented technology

Special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes

The invention relates to a special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes, belonging to the field of testing. Rapid diagnosis of fulminating infectious disease cholera with low cost is one of many expectations in the development of medical technology, and the invention provides a micro-fluidic chip for simultaneously detecting and rapidly diagnosing cholera by using multiple characteristic antibodies of cholera. The chip is provided with three micro liquid storage tanks, and the invention is characterized in that capillary passages in parallel configuration contain in the chip, the parallel configuration contains four micro-passages that are mutually in parallel, four magnetic bead chain working electrodes are respectively installed inside the four micro-passages, the surface substances of the four magnetic bead chain working electrodes are respectively four antibody substances that are cholera TP0821 antibody, cholera TP0319 antibody, cholera TP0624 antibody and cholera O139 mycoprotein antibody. The integrated structure and the appearances of specific magnetic bead chain working electrodes facilitate to improve the diagnosis efficiency of cholera diseases.
Owner:NINGBO UNIV

Micro-fluidic chip and micro-fluidic chip system for single cell analysis and single cell analyzing method

The invention relates to a micro-fluidic chip and a micro-fluidic chip system for single cell analysis and a single cell analyzing method. The micro-fluidic chip for single cell analysis comprises a transparent top cover, a microfluid channel, a substrate with a micropore array and a bottom plate with a cavity body, which are sequentially connected from top to bottom in a sealing manner, wherein the transparent top cover is provided with an inflow hole and an outflow hole which are used for communicating the outside and the microfluid channel, the micropore array of the substrate is arranged below the microfluid channel and is communicated with the microfluid channel, each micropore is internally provided with an electrode, each electrode is provided with a cathode and an anode, each micropore can contain a single cell only, the bottom of each micropore is provided with a channel which is communicated with the cavity body of the bottom plate, and the cross section of the channel is smaller than the size of each single cell. With the adoption of the micro-fluidic chip, the micro-fluidic chip system and the single cell analyzing method, the capturing, identifying and disruption processes of each single cell are integrated on one micro-fluidic chip; as a corresponding operation method is provided, the content of each single cell is rapidly acquired and accurately analyzed.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA

Cell sorter micro-fluidic chip based on immunomagnetic separation technology and application thereof in aspect of enrichment of rare cells

The invention discloses a cell sorter micro-fluidic chip based on the immunomagnetic separation technology and application thereof in the aspect of enrichment of rare cells, belonging to the technicalfield of micro-fluidic chips. The micro-fluidic chip, improved on the basis of the existing Y-type micro-fluidic chip, comprises a substrate and a cover-slip, wherein a Y-shaped micro-channel, a sample cell (1) and a buffer cell (2) respectively located at two end points of the upper part of the Y-shaped micro-channel, and an effluent cell (3) located at the end point of the lower part of the Y-shaped micro-channel are arranged on the substrate; and an antigen/antibody immunological reaction chamber (4) located on the micro-channel between the cross point (5) of the Y-shaped micro-channel andthe effluent cell (3) is further arranged on the substrate, wherein the width of the antigen/antibody immunological reaction chamber (4) is 2 to 5 times that of the micro-channel. The chip of the invention can effectively and rapidly fix the magnetic beads, increase the sample size of cells, improve the analysis flux, reduce the dead volume of sample introduction and reduce the retention and cross infection.
Owner:TSINGHUA UNIV

Micro-fluidic chip structure for flow cytometer, and preparation method of micro-fluidic chip

The invention discloses a micro-fluidic chip structure for a flow cytometer, and a preparation method a micro-fluidic chip. The chip comprises a sample liquid inlet 1, a column array structure 6, a conical focusing structure 7, a micro-channel 8, a detection zone 4, a flow expanding channel 9 and a waste liquid outlet end 5, wherein the column array structure 6 can play a role of filtering agglomerate protein impurities and other large biological solids inside sample liquid; and the conical focusing structure 7 has a focusing effect which is similar to that of the traditional sheath liquid system, so that cell granules flow inside the micro-channel 8 one by one; and the micro-channel 8 restricts the cells through the channel, so that the cells pass through the detection zone 4 one by one. By adopting the chip structure disclosed by the invention, the focusing effect without sheath flow liquid can be achieved; and blockage is not generated, so as to reduce use of the sheath flow liquid; and meanwhile, the situation that the cell granules pass through the detection zone one by one is ensured. The processing method of the micro-fluidic chip is convenient; the used bonding method is a common bonding method, and is simple and convenient to operate; and the used material PDMS (polydimethylsiloxane) is easy to process and has good biocompatibility.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

Full-automatic nucleic acid extraction and PCR amplification micro-fluidic chip and application method thereof

The invention provides a full-automatic nucleic acid extraction and PCR amplification micro-fluidic chip which comprises at least one main body, wherein the main body is distributed in equal distance around the chip; the main body comprises a nucleic acid extraction unit, a PCR amplification unit, a waste liquid unit and an exhaust unit, wherein the nucleic acid extraction unit is positioned on the main body and is used for extracting, washing and eluting nucleic acid in sequence under the driving of centrifugal force and capillary force; the PCR amplification unit is adopted for PCR reaction amplification; the waste liquid unit comprises a waste liquid cavity which is used for storing a waste liquid; the exhaust unit comprises exhaust holes and a plurality of exhaust channels; and the plurality of exhaust channels are communicated with the nucleic acid extraction unit, the PCR amplification unit and the waste liquid unit and are used for exhausting air. The invention discloses a micro-fluidic chip for achieving full-automatic nucleic acid extraction and PCR reaction through centrifugal force, capillary force and siphon phenomenon, the micro-fluidic chip has no integrated equipment such as pumps or valves, so that the manufacturing difficulty and cost of the chip are greatly reduced, and moreover the reliability of the chip is improved.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof

The invention relates to a centrifugal micro-fluidic chip for detecting pesticide residue and a preparation method thereof. The centrifugal micro-fluidic chip for detecting the pesticide residue is a disk type chip with a micro structure and a micro channel and consists of multiple layers of chips; the mixing, extracting, reacting, separating and developing process of a sample to be measured and a reagent is realized under the drive of a centrifugal force generated by rotating a centrifugal machine; and finally, the content of the pesticide residue in the sample is quantitatively detected by using an ultraviolet visible light spectrophotometer. The centrifugal micro-fluidic chip for detecting the pesticide residue can be used for directly sampling without pretreating the sample; the consumption of the sample and the reagent is low; a plurality of samples can be simultaneously treated and detected in parallel, and thus the integration, the micromation and the automation of the pesticide residue detection are realized; and the centrifugal micro-fluidic chip has the characteristics of economy, quickness and portability and provides a brand-new analysis technology for quickly detecting the pesticide residue of water bodies, foods and soil.
Owner:SUZHOU WENHAO MICROFLUIDIC TECH CO LTD

3D-printing-based machining method of three-dimensional micro-fluidic chip and printing device

The invention discloses a 3D-printing-based machining method of a three-dimensional micro-fluidic chip. The machining method comprises the following steps: (1) drawing a three-dimensional micro-runner figure; (2) layering three-dimensional runners in the three-dimensional micro-runner figure, and carrying out layer-by-layer slicing on the three-dimensional runners along the bottom surface direction parallel to a chip; (3) pouring a liquid-state chip material on a micro-runner solid structure at a previous layer, sequentially printing the micro-runner solid structure and pouring the chip material on each layer according to layering and slicing sequences of the runners, and sequentially finishing the shape printing of the runners, so as to obtain a three-dimensional runner solid structure; (4) dissolving the three-dimensional runner solid structure, so as to obtain the three-dimensional micro-fluidic chip. The invention further discloses a printing device for implementing the method. The 3D-printing-based machining method has the advantages that a machining process is simple, convenient and rapid, the production efficiency is high, the industrial mass production is easily realized, the obtained three-dimensional micro-fluidic chip does not need to be subjected to post-processing processes such as bonding, a packaging process is simplified, a three-dimensional micro-runner with a complex structure can be manufactured, and the shape and precision of the runner are controllable.
Owner:ZHEJIANG UNIV

Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue

InactiveCN104502617AAvoid preparation workQuick checkMaterial analysisEnzyme inhibitionPesticide residue
The invention provides a full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue, used for field detection of pesticide residue based on enzyme inhibition reaction. The full-automatic and high-throughput micro-fluidic chip system is mainly composed of a portable analytical detector and a disposable micro-fluidic chip; the plastic polymer material of the micro-fluidic chip is manufactured through existing micro-machining technology; the micro-fluidic chip is composed of a central clamping groove, an extraction chamber, a sample chamber, a reaction chamber, a detection chamber, a micro-groove, a micro-hole and a quality control bar code; reagents (comprising an extracting solution, enzyme, a colour developing agent and the like) required for detecting pesticide residue are fixed in the micro-fluidic chip; the functions of sampling automatically and quantitatively, distributing a fluid, carrying out biochemical reaction and molecular recognition and the like can be realized; a fluid operation and control module, a photoelectric detection module, a data analyzing and processing module and an information storage and communication module are integrated in the portable analytical detector; operation and control of biochemical reaction in the micro-fluidic chip and rapid analysis and detection of to-be-detected indexes and pesticide residue can be realized; and thus, the micro-fluidic chip system and the method provided by the invention are particularly applied to full-automatic and high-throughput detecting and screening of pesticide residue in the samples, such as fruits, vegetables, soil and water.
Owner:HANGZHOU TINKER BIOTECHNOLOGY CO LTD

Ion sensitive sensor and manufacturing method thereof

The invention relates to an ion sensitive sensor comprising a silicon oxide layer and a silicon substrate which are vertically stacked, a source electrode and a drain electrode arranged on the silicon oxide layer at an interval, and a graphene layer arranged on the surface of the silicon oxide layer and between the source electrode and the drain electrode; wherein the graphene layer is respectively connected with a gear shaping part of the source electrode and a gear shaping part of the drain electrode, and the surface layer of the graphene layer is penetrated with metal oxide particles; the ion sensitive sensor also comprises a micro fluidic channel layer which is erected on the gear shaping part of the source electrode and the gear shaping part of the drain electrode, and the gear shaping part of the source electrode and the gear shaping part of the drain electrode are coated with insulating layers; the micro fluidic channel layer is matched with the graphene layer to form a cavity for accommodating an object for measurement, and is opened with through holes; and suspended grid electrodes are inserted into the through holes and extend into the cavity. The ion sensitive sensor has a structure which is similar to a field effect transistor, and has advantages of high flux, high sensitivity, short response time and the like.
Owner:SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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