91 results about "Micro fluidic" patented technology

The present disclosure relates to a microfluidic chip and a microfluidic device. The microfluidic chip comprises: a sample inlet configured to receive a sample microfluid; a sample channel configured to communicate with the sample inlet to receive the sample microfluid from the sample inlet; a capillary pump configured to communicate with the sample channel to draw the sample microfluid through and out of the sample channel; and one or more liquid metering cavities, each of which is configured to communicate with the sample channel between the sample inlet and the capillary pump via a sample splitting channel corresponding to the liquid metering cavity to meter a preset volume of the sample microfluid corresponding to the liquid metering cavity from the sample channel, and is configured to communicate with the atmosphere via a first communication structure corresponding to the liquid metering cavity, wherein a cross-sectional area of the sample channel is greater than a cross-sectional area of the liquid metering cavity, the cross-sectional area of the liquid metering cavity is greater than a cross-sectional area of the sample splitting channel, and a cross-sectional area of the first communication structure corresponding to the liquid metering cavity on a side adjacent to the liquid metering cavity is greater than or equal to the cross-sectional area of the sample channel.

The application discloses a kind of visualization methods for microcosmic action mechanism of steam displacement heavy oil, which comprises: obtaining the porous structure information of rock sample;According to the structure information of rock sample, design pore network structure and make microfluidic chip;Build steam displacement heavy oil visualization implementation system, and adopt steam displacement heavy oil visualization implementation system to collect the phase distribution and temperature distribution of microfluidic chip in displacement process;Boundary extraction is carried out to experimental picture, and the dynamic change process of each phase in displacement process is obtained and the interaction relationship with temperature distribution change is obtained.The application designs pore network chip according to the pore structure information of rock sample, obtains the phase distribution and temperature distribution in displacement process, and is used to study the microcosmic phase distribution characteristics and temperature distribution characteristics of fluid in heavy oil reservoir reservoir, and then the microcosmic mechanism of reservoir fluid movement is explored, so as to provide a practical visualization method for the research of steam drive oil mechanism.

The invention relates to an embedded micro-fluidic active heat dissipation structure based on a diamond substrate and a preparation method, and belongs to the technical field of heat dissipation. The embedded micro-fluidic active heat dissipation structure based on the diamond substrate comprises the diamond substrate and a cover plate combined on one side of the diamond substrate; a micro-fluid channel system is formed from the surface of one side, close to the cover plate, of the diamond substrate to the interior of the diamond substrate, and the micro-fluid channel system comprises an inlet manifold, a plurality of groups of jet hole arrays, a plurality of micro-channels and an outlet manifold; a fluid disturbance structure is arranged in each micro-channel in the fluid flowing direction. According to the embedded micro-fluidic active heat dissipation structure disclosed by the invention, a micro-fluidic channel system with a specific shape is constructed in the diamond substrate, and jet reinforcement, disturbance enhancement and manifold shunting design are combined, so that the ultrahigh heat dissipation capability under low energy consumption is realized, the problem that the heat dissipation efficiency of an existing silicon-based micro-channel is limited is effectively solved, and the heat dissipation efficiency of the silicon-based micro-channel is improved. And an efficient, stable and integratable thermal management scheme is provided for a high-power electronic device.

An electrowetting system for actuating droplets of a first composition and of a second composition. The system includes: a plurality of electrodes configured to manipulate droplets of fluid in a microfluidic space, each electrode being coupled to circuitry which applies driving voltages to the electrode; and a processing unit operably connected to a look up table correlating drive sequences to chemical species and at least one composition parameter. The pulse sequence of driving voltages to move at least one of the droplets contains at least three consecutive positive pulses or is positively biased.

The application discloses a DNA molecule manipulation chip based on electric field and bending micro-channel coupling driving, which comprises a glass substrate and a structure layer, the structure layer is arranged on the glass substrate, at least one group of micro-fluid systems are arranged in the structure layer, two ends of the micro-fluid system are respectively communicated with a liquid inlet hole and a liquid outlet hole, the liquid inlet hole and the liquid outlet hole are vertically arranged through the structure layer, electrodes are respectively inserted into the liquid inlet hole and the liquid outlet hole, and the electrodes are used for applying direct current voltage to generate a spatial non-uniform electric field; a control method applied to the chip comprises the following steps: chip pretreatment, application of driving electric field, DNA molecule acceleration and polarization, DNA molecule positive dielectrophoresis focusing and migration; the application realizes high-efficiency mechanical-free focusing of the DNA molecules, and greatly improves the precision of subsequent optical detection and separation.

The application provides a double-rectangular-coil high-flux inductive oil detection device, which comprises a double-rectangular-coil microfluidic chip, a signal processing circuit and a data acquisition card, wherein the double-rectangular-coil microfluidic chip comprises an oil inlet, PDMS, a microfluidic channel, a glass slide, an oil outlet, a first rectangular excitation coil, a second rectangular excitation coil, a resonance capacitor and a thin piece of permalloy; the first excitation coil and the second excitation coil are connected in parallel on a waveform generator with a voltage of U in ; the resonance capacitor is connected in parallel with the first excitation coil and the second excitation coil, and the two ends of the resonance capacitor are connected with the signal processing circuit; an excitation signal U in is a sinusoidal signal sent by the waveform generator; an output induction signal U out is also a sinusoidal alternating current signal, which is converted into a direct current signal through the signal processing circuit, and finally converted into a digital signal and stored in a computer through the data acquisition card. The application can greatly improve the microfluidic channel flux of the microfluidic chip under the premise of almost no loss of detection accuracy and stable detection results.

The present application provides a kind of microfluidic switching valve and multi-sample injection analysis system, microfluidic switching valve includes first valve body and second valve body, first valve body is provided with a first interface, a plurality of second interfaces and a third interface, second valve body is provided with first channel and second channel, multi-sample injection analysis system includes injection device, microfluidic switching valve, analysis equipment, cleaning fluid supply device;First interface is communicated with analysis equipment;First interface can be selectively communicated with any one of second interface or third interface, other second interfaces and / or third interfaces not communicated with the inlet of first interface are mutually communicated with each other;Injection device has multiple sample outlets, each sample outlet is communicated with a second interface correspondingly.This microfluidic switching valve of the present application has less interface, and can carry out pipeline cleaning after injection, reduce the cross contamination between samples.

The present invention provides for a process for synthesis of carbon beads comprising sub-micron size, micron size or milli size. The process enables modulation of the viscous slurry for synthesis of the carbon beads with improved physico-chemical properties. The process enhances ability of the carbon beads to withstand extreme pH and high temperatures. The present invention also provides a composition for synthesis of the carbon beads. The present invention also provides a microfluidic droplet generator for synthesizing the carbon beads. The carbon beads synthesized by the present invention are applicable in separation, filtration, purification, wires and cables, electrodes, sensor, composite and additive manufacturing, pharmaceutical delivery applications.

A device for analyzing a sample liquid is provided, the device including a microfluidic test card and a microfluidic chip for processing sample liquid presented from the microfluidic test card and returning the processed sample fluid to the microfluidic test card. The microfluidic test card includes a sample inlet configured to receive sample liquid, first and second pre-treatment test reagent channels having first and second test reagent outlets, respectively, for presenting test reagents to the microfluidic chip, a pre-treatment sample channel in fluid communication with the sample inlet for receiving sample liquid from the sample inlet and having a sample liquid outlet for presenting sample liquid to the microfluidic chip, first and second processed sample analysis channels for receiving processed sample liquid from the microfluidic chip, wherein the first and second processed sample analysis channels include first and second analysis zones, respectively, for analyzing the processed sample liquid, and a microfluidic chip contact region including the sample liquid outlet and the first and second test reagent outlets configured for connection and fluid communication with the microfluidic chip.

The invention discloses a sensing probe assembly, a fluidic optical fiber sensing system and a preparation method, and relates to the cross technical field of optical fiber sensing technology and microfluidic detection. One free end of a single-mode optical fiber in the sensing probe assembly is fixed to the outer wall of a first square capillary tube through ultraviolet curing glue, and a second square capillary tube is coaxially or parallelly inserted into an inner cavity of the first square capillary tube. A uniform air gap is formed between the inner wall of the first square capillary tube and the outer wall of the second square capillary tube, a microfluid channel is formed in the middle of the second square capillary tube, and broadband light beams emitted from the single-mode optical fiber are vertically incident. The micro-fluidic chip sequentially penetrates through the ultraviolet curing adhesive, the upper wall of the first square capillary tube, the air gap and the upper wall of the second square capillary tube, enters the micro-fluidic channel filled with liquid to be detected, then penetrates through the lower wall of the second square capillary tube and finally reaches the lower wall of the first square capillary tube. The probe has the advantages of high detection sensitivity, strong light beam coupling stability and the like.

This invention relates to a microfluidic chip driven by acoustically excited droplet-shaped microbubbles and its fabrication process. A droplet-shaped microbubble array is obtained through microfluidic chip structure design. Under acoustic excitation, the microbubbles oscillate, generating asymmetric acoustic-flow vortices, which in turn directionally drive the microfluidic fluid within the microchannels. The flow rate can be linearly controlled by adjusting the driving voltage, offering advantages such as non-contact, pollution-free, and highly efficient directional driving. The fabrication process is based on MEMS technology, employing nitrogen plasma bonding and soft lithography, resulting in low cost and suitability for mass production. This invention solves the problem of the lack of directionality in traditional symmetrical acoustic-flow vortices and can be widely applied in fields such as biomedicine, drug delivery, and chemical analysis.

The present application relates to the technical field of biomedical engineering, and particularly discloses a collagen spraying control method and system for instant cross-linking forming, wherein the present application realizes the synchronous spraying of collagen and cross-linking agent through micro-fluid dynamics control, and solves the problems of early cross-linking, pipeline blockage and unstable film forming quality in the traditional method. The present application first generates a driving time sequence, independently controls the delivery of collagen precursor liquid and cross-linking agent solution, and constructs a stable micro-fluid isolation interface to prevent early cross-linking; then, the isolation interface is torn by using a carrier gas flow, a cross-linking micro-mist droplet cloud is formed and projected to a receiving substrate, and instant cross-linking forming is triggered to generate a collagen cover film; the system also has dynamic correction and self-cleaning functions, and ensures the continuous and stable operation of the spraying operation. The present application improves the preparation efficiency and quality of biological materials, and provides a new solution for the application of biological materials.

The utility model discloses a kind of chromatography devices of flow rate and time controllable, belong to medical instrument technical field, including liquid storage bottle, the upper end of liquid storage bottle is provided with inlet, the lower end of liquid storage bottle is provided with pipeline one, the lower end of pipeline one is provided with microfluid pump, the lower end of microfluid pump is provided with chromatography column, the lower end of chromatography column is provided with pipeline two, the lower end of pipeline two is provided with detector, the utility model is by being provided with the structure that liquid flow rate and reaction time can be controlled, the accurate control of liquid flow is realized, controller can automatically adjust the speed of microfluid pump and electromagnetic valve start-stop time according to preset experimental parameter, ensure that liquid flows at the speed set, and complete reaction within predetermined time, this design improves the accuracy and repeatability of experiment, especially suitable for the chromatography experiment that needs accurate control flow rate and time, can effectively improve experimental efficiency and the reliability of data.

The invention relates to a microfluidic device (100,200) for handling a fluid sample by dividing it into a plurality of aliquots, with at least one microfluidic well (102) configured to receive the fluid sample. The at least one microfluidic well comprising an inlet end (108), an outlet end (112) and a microfluidic circuit (104) with a plurality of microchambers (106). The inlet end and the outlet end are respectively coupled to the microfluidic circuit via at least one microfluidic channel (110,110',110",110‴). The plurality of microchambers is arranged such that each microchamber is at least indirectly via one or more further microchambers fluidly coupled with the inlet end and the outlet end such that each microchamber is configured to receive sample fluid from the inlet end via the at least one microfluidic channel and to provide gas displaced by the sample fluid downstream to the outlet end. Furthermore, a protective layer (114,214) is applied to the at least one microfluidic well for sealing the inlet end and / or the outlet end of the microfluidic well, wherein the protective layer is at least partially permeable to gas.

The present disclosure relates to a sandwich assay method for detecting the presence of a target molecule in a microfluidic device, the method comprising: a) Providing the microfluidic device comprising a micro- or nanoscale channel, wherein an affinity probe is immobilized to an inner surface of the micro- or nanoscale channel; b) Providing a first solution comprising the target molecule into the microchannel; c) Providing a second solution with detection enhancement probe into the microchannel, the detection enhancement probe comprising a nanoparticle and one or more detection probes with affinity for the target molecule; and d) Detecting the presence of the detection enhancement probe along with the target molecule and the affinity probe by recording the changes in streaming current or potential. The present disclosure furthermore relates to a displacement assay method and to kits for use in the methods.

The present invention concerns the field of microreaction devices and of micro-process engineering. It particularly involves devices having micro-channels (internal chambers of micrometric to submicrometric dimensions) for conveying chemical or biochemical mixtures and / or reactions. More specifically, such devices are optimized to achieve high temperature and pressure stresses (i.e. 500° C. and 500 bar). For observation and analysis purposes, the microreaction devices have a wide range of transparency in terms of wavelengths. The subject matter of the present invention relates to a microfluid or microreactor device made of transparent sapphire, preferably in the wavelength range of 150 to 6500 nm, its manufacturing method and to its use.

Provided are a microfluidic chip (304) and a usage method therefor, a microfluidic system, and a manufacturing method for a conductive cover plate (4). The chip (304) comprises an extraction assembly (1) and an amplification assembly (2). The extraction assembly (1) comprises a lysis chamber (100), a first valve chamber (101), washing chambers (102, 103), and a second valve chamber (104) that sequentially connect with each other. The amplification assembly (2) comprises a chip substrate (6), a barrier layer (5), and a conductive cover plate (4). The barrier layer (5) is located between the chip substrate (6) and the conductive cover plate (4). The barrier layer (5), the chip substrate (6), and the conductive cover plate (4) form an elution chamber (21), a liquid path chamber (22), and an amplification chamber (23) that sequentially connect with each other. The elution chamber (21) connects with the second valve chamber (104) through a first channel (24). An electrode array (7) is arranged on the chip substrate (6). A side of the conductive cover plate (4) adjacent to the chip substrate (6) is provided with a cavity (400) having an opening at a bottom, and the elution chamber (21) contains the cavity (400). The electrode array (7) is located right under the elution chamber (21), the liquid path chamber (22), and the amplification chamber (23). The amplification assembly (2) further comprises a storage chamber (20) for storing a surfactant, and the storage chamber (20) connects with the elution chamber (21) through a second channel (25). The liquid path chamber (22) comprises a liquid storage chamber (220), and the liquid storage chamber (220) is adjacent to the elution chamber (21). The cavity (400) has a thickness of 0.6 mm to 2 mm in a thickness direction of the microfluidic chip (304).

This invention relates to microfluidic devices, and more particularly to a sperm sorting device and a sperm guiding device. A sperm sorting device is provided, comprising: an inlet chamber, an outlet chamber located radially inside the inlet chamber, and a channel connecting the inlet and outlet chambers. The channel includes blind branches with a radius of curvature between 50 μm and 75 μm. A sperm guiding device is also provided, comprising an inlet opening and an outlet opening, and blind branches with a radius of curvature between 50 μm and 75 μm. The technical advantage of this invention is the reduction of sperm backflow.

This invention discloses a microfluidic chip and its fabrication method for constructing modular cell cultures, relating to the field of cell culture technology. The chip includes an upper plate and a lower plate. The upper plate includes a first flow channel and a second flow channel, while the lower plate includes multiple spaced-apart culture chambers, each with a different cross-sectional shape. This allows each chamber to be independently formed into cultures of different shapes. The cultures can be stacked and combined after removal to construct a multi-cell co-culture model with functional partitions, significantly improving structural complexity and biomimicry. The upper plate slides to switch the connection between the first and second flow channels and the culture chambers, enabling inoculation and culture modes. During inoculation, cultured cells are injected into the culture chamber through the first flow channel; during culture, the flow channel is switched to perfuse the culture chamber with culture medium, avoiding damage to the culture during residual gel removal. This simple operation improves the success rate and enhances the reliability of experimental results.

This invention discloses a device and method for rapid generation of mid-phase microemulsions and high-throughput screening of formulation systems, belonging to the field of oilfield chemistry and microfluidics. The device includes at least one oil-phase micro-injection pump for injecting the oil phase, at least one aqueous-phase micro-injection pump for injecting the aqueous phase (containing surfactants), syringes mounted on the two micro-injection pumps, a microfluidic chip, an effluent collector, a constant-temperature heater, and a temperature probe. Utilizing this device, by precisely controlling the flow rate, ratio, and system temperature of the oil and aqueous phases entering the chip, rapid and efficient mixing and mass transfer of fluids are achieved within micron-scale channels, significantly shortening the time required to reach phase equilibrium. This invention solves the technical problems of long equilibrium times, cumbersome operation, low efficiency, and high reagent consumption associated with traditional test-tube methods for preparing mid-phase microemulsions when screening surfactant formulations suitable for enhancing oil recovery.

The invention relates to a microfluidic device (100,200,700) for handling a fluid sample by dividing it into a plurality of aliquots, with at least one microfluidic well (105) configured to receive the fluid sample. The at least one microfluidic well comprising an inlet end (120), an outlet end (125) and a microfluidic circuit (110) with a plurality of microchambers (112). The inlet end and the outlet end are respectively coupled to the microfluidic circuit via at least one microfluidic channel (130,130',130'',130'''). The plurality of microchambers is arranged such that each microchamber is at least indirectly via further microchambers fluidly coupled with the inlet end and the outlet end such that each microchamber is configured to receive fluid from the inlet end via the at least one microfluidic channel and to provide gas displaced by the fluid downstream to the outlet end. Furthermore, a storage section (140,240,740) with a microfluidic storage circuit (142,242,342,742) is provided in order to store fluid that does not reach and / or flows beyond the microchambers of the microfluidic well during a filling process of the microfluidic device and can therefore not be provided at the intended reaction space, wherein a storage opening structure (144,344) is provided in the storage section.

An electronic device includes a substrate, a thin film transistor, a micro pump, and a microfluidic platform. The thin film transistor is disposed on the substrate. The micro pump is disposed on the substrate and electrically connected to the thin film transistor. The microfluidic platform is disposed on the substrate and coupled to the micro pump. The micro pump is used to transport a sample to be tested to the microfluidic platform.

This invention discloses a biochip system integrating GUVs preparation and capture, cell membrane model construction and analysis, consisting of three sections: a preparation section, a stabilization section, and a capture and analysis section. The preparation section includes an internal aqueous solution channel, an oil-in-water droplet delivery channel, and a GUVs forming channel connected sequentially. Lipid solution channels are located above and below the right end of the internal aqueous solution channel, and external aqueous solution delivery channels are located above and below the end of the oil-in-water droplet delivery channel. The stabilization section is a serpentine flow channel. The capture and analysis section also includes a serpentine flow channel, with GUVs capture microcavity structures arranged in an array from top to bottom on the right side of the vertical tube of the serpentine flow channel. This invention utilizes microfluidic injection to precisely control the morphology and size of GUVs, combined with microstructures embedded in the serpentine flow channel to capture GUVs. The serpentine channel stabilizes the fluid and exerts high shear force, stabilizing the GUVs and reducing oil phase residue. No additional centrifugation collection and screening processes are required, enabling integrated research on cell membrane model construction and analysis.

A microfluidic device includes a microfluidic channel, a first impedance sensor positioned along the microfluidic channel, a second impedance sensor positioned along the microfluidic channel, and a pair of electrodes for electroporating a cell, the pair of electrodes positioned in the microfluidic channel between the first impedance sensor and the second impedance sensor.

The systems and methods described herein relate to performing one or more highly multiplexed cell assays. In some embodiments, one or more channels of a fluidic device are provided with a photopolymerizable polymer precursor and cells randomly arranged on a surface, followed by measurement of cell position by a detector, synthesis of a hydrogel chamber via photopolymerization to encapsulate individual cells, and loading of assay reagents into the channels. An assay signal indicating the desired cellular characteristics is generated for each of the encapsulated cells.

Microfluidic device for culturing cells A method for forming a microfluidic device for culturing cells, the method comprising: providing a microfluidic device comprising a first chamber and a second chamber fluidically connected to the first chamber by a first porous structure comprising through holes for allowing fluid to pass through, said first porous structure physically separating the first chamber and the second chamber, wherein each of said chambers has photosensitive inner walls having a first property; and then irradiating the microfluidic device with light to which the photosensitive inner walls are sensitive, in such a way that the first porous structure acts as a mask during irradiation, thereby changing the surface property of the exposed surfaces and imparting a different surface property in the first chamber compared to the second chamber.

Methods and apparatus for the creation and use of chiplets loaded with different reagent embedded in gel carrier materials are described herein. The chiplets are created using a microfluidic device. The reagents embedded in the gel carrier materials are drawn through channels of the microfluidic device and then fixed in place within the microfluidic device. After fixing the reagents in place, generally by a reduction in temperature, one or more chiplets are separated from a region of interest portion of the microfluidic device. The chiplets can then be packaged and sold to end-users for various that would traditionally require microfluidic devices. End-user can then affix one or more of the chiplets to a sample without needing to perform a microfluidic flow operation.

This invention discloses a low-dissipation biomimetic microchannel network system and its manufacturing method based on fluid-structure interaction (FSI), belonging to the field of high-efficiency thermal management and microfluidic transport technology. Addressing the technical challenges of existing microchannels where surface roughness after additive manufacturing leads to a dramatic increase in pressure drop, and the inability of conventional mechanical polishing to handle complex three-dimensional blind cavities, this invention proposes a system deeply coupled with topological structure and rheological polishing. The various branching nodes of this microchannel network substantially follow the optimal mass transfer law of fluid mechanics (Murray's law) for multi-stage variable cross-section fractal design. During manufacturing, the physical property of a non-Newtonian fluid medium exhibiting constant wall shear stress within a branch network conforming to this law is utilized to achieve constant-rate, dead-angle-free mirror polishing of the complex network's inner surface. Ultimately, the absolute roughness equivalent of the microchannel's inner surface is strictly less than the thickness of the laminar viscous sublayer under design conditions, completely eliminating morphological shock waves at the microscopic level and achieving extremely low fluid pressure drop and highly uniform mass and heat transfer.

The application relates to the technical field of space on-orbit filling, in particular to a space low-temperature propellant filling refrigeration device and method, which comprises a fluid inlet, a micro-fluid heat exchanger assembly, a fluid outlet, a refrigeration unit, a radiator, an adiabatic structure and a measurement and control assembly, wherein: the inlet end of the micro-fluid heat exchanger assembly is connected with the fluid inlet; the fluid outlet is connected with the outlet end of the micro-fluid heat exchanger assembly; the refrigeration unit comprises an expander and a compressor which are connected in sequence; the expander is connected with the micro-fluid heat exchanger assembly; the radiator is connected with the compressor; the adiabatic structure is wrapped outside the micro-fluid heat exchanger assembly; and the measurement and control assembly is electrically connected with the refrigeration unit and the radiator respectively. The micro-fluid heat exchanger assembly of the application adopts a micro-fluid channel as a fluid path, greatly improves the cold heat exchange area, shortens the cold heat exchange distance, enhances the active refrigeration heat exchange efficiency, reduces the weight and volume of the heat exchanger and reduces the refrigeration machine power consumption.