825 results about "Micro fluidic" patented technology

The invention belongs to the technical field of construction of super-hydrophobic surfaces, and particularly relates to a preparation method of a super-hydrophobic coating with a multistage micro-nano structure. Comprising the following steps: pretreating a substrate; slowly adding the F-SiO2 nanoparticle dispersion liquid into the fluorine-containing glue solution under the synergistic treatment of ultrasonic and magnetic stirring to obtain an F-SiO2 / fluorine-containing glue composite solution; the method comprises the following steps: determining the actual glue coating amount based on the theoretical glue demand amount of a PDMS template, and coating the F-SiO2 / fluorine-containing glue composite solution on the surface of a substrate through spin coating to form a wet film; coining the wet film by using a PDMS template, and stripping the template after ultraviolet curing to obtain the super-hydrophobic coating with the multistage micro-nano structure. Based on an imprint method with insufficient glue amount, synchronous construction of a micro / nano dual-scale structure is achieved by accurately controlling the ratio of the volume of the coating liquid to the volume of the pore of the template and combining the curing shrinkage characteristic of the polymer, and the method has application potential in the fields of anti-icing, microfluid devices, marine antifouling and the like.

The utility model provides a microfluid sample detection device which comprises a fluid inlet, a microfluid channel, a fluid outlet, a reaction area, a liquid collecting area, a pump body and a valve body, the valve body comprises a substrate, a double-sided colloid and a flexible diaphragm, a cavity is formed between the upper surface of the plastic substrate and the lower surface of the flexible diaphragm, the cavity is opened when the flexible diaphragm is loosened, and the cavity is closed when the flexible diaphragm is tensioned; the valve body comprises a first valve body, a second valve body, a third valve body and a fourth valve body, the second valve body and the third valve body are arranged between the pump body and the microfluid channel and located on the downstream portion of the first valve body, and the working states of the third valve body and the fourth valve body are opposite. Moreover, the parallel scheme further comprises a conductive system composed of electrodes, the conductive system at least comprises two electrodes, and the electrode sensing ends are located at the two ends of the reaction area respectively. The microfluidic sample detection device is loaded in a detection device to form a microfluidic sample detection system.

The present application provides a microfluidic device, comprising at least one microfluidic channel unit, each microfluidic channel unit comprising a microfluidic channel configured to contain a fluid and comprising a plurality of independent compartments. Each microfluidic channel unit comprises a first side wall and a second side wall opposite and spaced apart from the first side wall, a plurality of first extensions formed on the first side wall extending toward the second side wall without abutting the second side wall, a plurality of second extensions formed on the second side wall extending toward the first side wall without abutting the first side wall, the plurality of first extensions and the plurality of second extensions being alternately arranged in the microfluidic channel. A compartment is formed in at least one first extension or at least one second extension. The compartment has an opening positioned such that the fluid does not directly flow into each compartment. The microfluidic device of the present application has a stable isolated environment to facilitate various reactions and prevent micro-objects in the compartments from escaping.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention discloses a preparation method and equipment of three-chamber eccentric parallel structure nanofibers. The preparation method comprises the following steps: simultaneously carrying out high-voltage electrostatic spinning on three spinning solutions; the two spinning solutions are respectively divided into microfluids to be led out through an array formed by two sets of series metal capillary tubes; the other spinning solution is divided into microfluids to be led out through a plurality of bottom through holes of the insulating container; the first series of metal capillary tubes are inserted into the second series of metal capillary tubes to form a series of metal capillary eccentric sleeves; the series of metal capillary eccentric sleeves penetrate through the insulating container and extend out of one side of the interior of the bottom through hole to form a combined eccentric outlet of three strands of spinning solutions; three spinning solutions are guided into a high-voltage electrostatic field in an eccentric parallel mode through the combined eccentric outlet, and the three-chamber eccentric parallel structure nanofiber is formed through single-step direct stretching. According to the method, the capability of preparing the three-chamber eccentric parallel structure characteristic nanofiber with a complete structure in a single-step, batch and large-scale manner is endowed.

The utility model provides a microfluid sample detection device and a microfluid sample detection system. The microfluid sample detection device comprises a fluid inlet, a microfluid channel, a fluid outlet, a reaction area, a liquid collecting area, a pump body, a valve body and a lock body, the valve body comprises a substrate, a double-sided colloid and a flexible diaphragm, a cavity is formed between the upper surface of the plastic substrate and the lower surface of the flexible diaphragm, the cavity is opened when the flexible diaphragm is loosened, and the cavity is closed when the flexible diaphragm is tensioned; the valve body comprises a first valve body and a second valve body, the second valve body is arranged on the downstream of the first valve body, and the working states of the first valve body and the second valve body are opposite; the lock body is arranged on the downstream of the second valve body. Moreover, the parallel scheme further comprises a conductive system composed of electrodes, the conductive system at least comprises two electrodes, and the electrode sensing ends are located at the two ends of the reaction area respectively. The microfluidic sample detection device is loaded in a detection device to form a microfluidic sample detection system.

The invention discloses a heat dissipation enhanced semiconductor packaging method, which comprises the following steps of: performing deep silicon etching to form a vertical through hole; insulation and metallization are carried out; thinning the wafer; performing hybrid bonding and stacking of multiple layers of chips; a heat dissipation design is adopted to form a vertical heat dissipation path; a three-dimensional thermal through hole network: adding pure copper thermal through holes in a non-functional area; performing microfluid cooling integration; molding and packaging; and thermal performance verification: verifying that the junction temperature can be controlled within 85 DEG C through infrared thermal imaging and finite element simulation. The TSV with the depth-to-width ratio of 10: 1 can be realized by adopting the Bosch process; a three-dimensional integrated TSV array supporting HBM and other high-speed memories is embedded into diamond or graphene heat dissipation columns, a low-heat-resistance vertical heat dissipation path is formed by combining a three-dimensional pure copper heat through hole network, and local hot spots are effectively dispersed; the high-thermal-conductivity epoxy molding compound and the embedded copper block enhance the overall mechanical strength and thermal diffusivity, so that the product can dissipate heat efficiently, and the overall area is not easy to influence.

An example one-time-open microfluidic valve can include an inlet microfluidic channel in a first elevation plane and an outlet channel in a second elevation plane. An upstream end of the outlet channel can overlap a downstream end of the inlet microfluidic channel. A capillary nozzle opening can connect the inlet microfluidic channel to the outlet channel at the overlapping ends. The capillary nozzle opening can have an outlet width that is smaller than a width of the outlet channel. A fluid actuator can be positioned to eject a fluid in the inlet microfluidic channel through the capillary nozzle opening into the outlet channel.

The utility model discloses a microfluid device and a system for researching abnormal migration of solute of a fractured porous medium system. The microfluid device for studying abnormal migration of the solute of the fractured porous medium system comprises a chip and a substrate, wherein the lower surface of the chip is bonded with the upper surface of the substrate; a crack and a porous medium area distributed around the crack are patterned on the lower surface of the chip; the porous medium area comprises a plurality of cylinders, gaps are reserved among the cylinders, and flow channels communicated with the cracks are formed; and a part or all of the cylinders in the porous medium area are provided with notches serving as blind ends. The microfluid system for studying abnormal migration of the solute of the fractured porous medium system comprises a fluid injection device, the microfluid device and a fluid collection device which are connected in sequence.

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

The utility model relates to the technical field of microfluidics, and discloses a multi-stage micro-fluidic chip, which at least comprises a first-stage collision fluid channel and a second-stage collision fluid channel, and a mixing channel of the upper-stage fluid channel is arranged as a premixing fluid channel of the lower-stage fluid channel; the first-stage collision fluid channel comprises a first premixed fluid channel and a second premixed fluid channel, a first-stage fluid collision channel is arranged between the first premixed fluid channel and the second premixed fluid channel, and the first-stage fluid collision channel enters the second-stage premixed fluid channel after collision; a second-stage fluid collision channel is arranged between the third premixing fluid channel and the fourth premixing fluid channel, and the second-stage fluid collision channel enters the mixing channel after collision. The multi-stage micro-fluidic chip has the advantages of high mixing efficiency, low shear damage, easiness in integrated packaging and batch manufacturing and the like, and the micro-fluid mixing performance and the system practicability are remarkably improved.

The invention relates to a consumable device for sweat-based monitoring, the consumable device comprising a proximal part configured to contact with a user, and further comprising: a) a sweat induction means on the proximal part, comprising: i. a sweat promoter configured to contact the skin of a user, and ii. a microfluidic structure; and b) a sweat interrogation means, located distally to the sweat induction means and comprising: i. a sample area; and ii. a passive fluid pump configured to induce the flow of the liquid through the sample area. The microfluidic structure is configured to convey fluid from the sweat promoter to the sample area. The invention also relates to a sweat-monitoring system comprising: a consumable device as above and a sweat-based monitoring device, the sweat-based monitoring device comprising sensing means and processing means.

The present disclosure provides bioreactors and methods of producing the same. Bioreactors of the present disclosure may include a macrostructure comprising a plurality of fluidically connected minimodules that form a plurality of channels. A channel may comprise a gas permeable layer configured to provide gas diffusion to or from the channel. The macrostructure may be manufactured using three-dimensional printing of a resin. The resin may comprise a polymerizable component, photoinitiator, thermocuring agent, or any combination thereof. One or more surfaces of the microfluidic macrostructure may comprise a biocompatible coating.

The invention relates to the technical field of greenhouse cultivation, in particular to an intelligent vacuum glass thermoelectric greenhouse which comprises a vacuum glass plate, a collecting system, a circulating system and a control system. The vacuum glass plates are arranged at the top and the periphery of the mounting framework; the collecting system is connected with a vacuum glass plate at the top of the mounting framework; the circulating system is connected with the collecting system; the control system is electrically connected with the circulating system; through the vacuum glass plate integrated with the perovskite solar cell layer, the Low-E film and the microfluid channel, solar energy can be accumulated in winter, and heat can be efficiently supplied to the interior of the greenhouse in a one-way mode; rainwater can be collected and circulated in the micro-fluid channel for active cooling, meanwhile, strong light direct radiation in summer is converted into soft scattering, and the seedling cultivation environment is guaranteed. The problems that energy consumption of cold and hot regulation and control of a vacuum glass greenhouse in summer and winter is high, and the generating capacity of a traditional solar photovoltaic panel cannot effectively maintain a temperature regulation and control system are solved.

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

Some embodiments of the technology disclosed herein relate to a system having a hydrodynamic separator element defining an element inlet and an element outlet. The element outlet has a first element outlet and a second element outlet. The hydrodynamic separator element has a plurality of curved microfluidic channels in fluid communication. Each of the plurality of microfluidic channels are arranged to operate in parallel. Each microfluidic channel defines a channel inlet downstream of the element inlet and a channel outlet having a first channel outlet upstream of the first element outlet and a second channel outlet upstream of the second element outlet. A flow characteristic sensor is in sensing communication with the separator element. A controller is in data communication with the flow characteristic sensor, where the controller is configured to provide a first alert upon the flow characteristic being outside a first threshold.

The invention discloses a reduced graphene oxide biochip based on a piezoelectric substrate and a preparation method and application thereof, the reduced graphene oxide biochip based on the piezoelectric substrate comprises a sound transduction unit and at least one detection unit connected to the sound transduction unit, and a microfluid channel layer is arranged between the sound transduction unit and the detection unit. A reduced graphene oxide layer is directly formed on the surface of a piezoelectric layer of a detection unit, a biological recognition element is fixed on the reduced graphene oxide layer, and the biological recognition element is used for being combined with target biological molecules to generate a reaction product with electron donating and receiving capacity. On the basis, the acoustic transduction electrode design of a concentric interdigital electrode structure is adopted, and the acoustic surface wave intensity which is more concentrated compared with other electrode structures is generated by utilizing the focusing effect of the concentric electrode structure; the flow velocity of the fluid to be measured in the microfluid channel is faster, and the directivity is stronger and more concentrated.

A light illumination and collection device (100; 200) for a micro-fluidic system comprises: a flow channel (110; 210) configured to extend in a first plane and allow flow of a sample through the flow channel (110; 210), a light source (120; 220) configured to transmit illumination light along an optical axis extending through a cross-sectional plane of the flow channel (110; 210) for causing light interaction between the illumination light and the sample to form sample information carrying light; a light collecting waveguide (130; 230) configured to extend in a second plane parallel to the first plane, wherein the light collecting waveguide comprises a light inlet (132; 232) configured to receive the sample information carrying light, wherein the light inlet (132; 232) and the light collecting waveguide (130; 232) are rotated in relation to a projection of a normal of the cross-sectional plane onto the second plane.

The present disclosure is drawn to inertial pumps. An inertial pump can include a microfluidic channel, a fluid actuator located in the microfluidic channel, and a check valve located in the microfluidic channel. The check valve can include a moveable valve element, a narrowed channel segment located upstream of the moveable valve element, and a blocking element formed in the microfluidic channel downstream of the moveable valve element. The narrowed channel segment can have a width less than a width of the moveable valve element so that the moveable valve element can block fluid flow through the check valve when the moveable valve element is positioned in the narrowed channel segment. The blocking element can be configured such that the blocking element constrains the moveable valve element within the check valve while also allowing fluid flow when the moveable valve element is positioned against the blocking element.

The invention discloses a micro-through-hole structure polymer metallization method for guided wave transmission, and belongs to the technical field of photoelectric device processing, a polymer substrate is used for replacing a traditional all-metal material, and the micro-through-hole structure polymer metallization method for guided wave transmission is achieved through the multi-stage processes of plasma activation, chemical coarsening, sensitization, chemical plating, electroplating and the like. And forming a compact and high-adhesion metal layer on the inner surface of the through hole of the polymer substrate. In the process, a microfluid pump or a peristaltic pump is adopted to drive the solution to circularly flow in the through hole and the microcavity. According to the micro-through-hole structure polymer metallization method for guided wave transmission, dynamic flowing of a chemical plating solution in a micro-through hole and an internal cavity is achieved by introducing a micro-fluid pump or a peristaltic pump, and therefore a uniform, compact and firmly-attached metal layer is obtained.

The invention discloses a core-sheath / parallel structure hybrid nanofiber membrane, a preparation method, a spinning head and a high-voltage electrostatic spinning device. The spinning head comprises a transversely-arranged cylindrical spinning solution storage device with spinneret orifices in the bottom end and a plurality of stainless steel capillary tubes penetrating through the cylindrical spinning solution storage device. And the capillary tubes are gathered above the spinning solution storage device to form two common fluid inlets. Part of the capillary tubes and spinneret orifices in the bottom of the cylindrical spinning solution storage device are arranged in parallel to form parallel outlets of two fluids; the other part of the stainless steel capillary tube directly passes through the center of the spinneret orifice of the cylindrical spinning solution storage device to form a coaxial outlet of the two fluids; and the parallel outlets and the coaxial outlets are uniformly distributed in a staggered manner at the bottom of the transverse storage device. According to the spinning device, an outlet jointly formed by a spinneret hole in the bottom of a spinning head and a metal capillary tube serves as a template, different multi-strand microfluid combinations are guided into a high-voltage electrostatic field, and the core-sheath / parallel structure hybrid nanofiber membrane is directly prepared in batches in a single step.

A surface plasmon resonance sensor system includes a sensor plate having a quartz glass substrate and a copper film sputtered onto the substrate and annealed for about two hours. A quartz prism is attached to a surface of the sensor plate, and a microfluidics unit containing carrier fluid is attached to the opposite surface. A target analyte reservoir is connected through a pump to inject target analytes into the microfluidics unit. A binder is attached to the copper film and binds to target analytes. A laser transmits a beam into the quartz prism at an incident angle that generates surface plasmons resonating with the copper film and reflects a phase shifted laser beam. Receive optics and a charge coupled device convert the phase shifted beam to electrical signals. A computing unit processes the electrical signals to compute refractive index changes based on phase shifts to indicate target analyte detection.

A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.

The invention belongs to the technical field of biological detection, and particularly relates to a primer probe pair, a microfluidic chip and a kit for detecting pathogens of vector infectious diseases based on a TaqMan microfluidic chip technology as well as a preparation method and application of the primer probe pair. Based on a TaqMan microfluidic chip technology, the invention provides a detection method for simultaneously detecting 28 kinds of pathogens of the disease-borne infectious diseases, and the related pathogens of the disease-borne infectious diseases comprise viruses, bacteria and parasites, and are wide in range and large in difference. The detection method provided by the invention has a low detection limit (5-20 copies / [mu] L); when the method is applied to an actual sample, the sensitivity, specificity, accuracy and precision are high, and compared with a gold standard method, the consistency is good; and compared with a traditional qPCR method, the method has the advantages that the detection time efficiency and the manpower and material consumption are greatly reduced, and the method is particularly suitable for disease monitoring and emergency disposal scenes with undefined pathogen spectrum, need of rapid and comprehensive troubleshooting and the like, and has a good application prospect.

The present disclosure relates to a microfluidic chip and a microfluidic device. The microfluidic chip comprises: a sample inlet configured to receive a sample microfluid; a sample channel configured to communicate with the sample inlet to receive the sample microfluid from the sample inlet; a capillary pump configured to communicate with the sample channel to draw the sample microfluid through and out of the sample channel; and one or more liquid metering cavities, each of which is configured to communicate with the sample channel between the sample inlet and the capillary pump via a sample splitting channel corresponding to the liquid metering cavity to meter a preset volume of the sample microfluid corresponding to the liquid metering cavity from the sample channel, and is configured to communicate with the atmosphere via a first communication structure corresponding to the liquid metering cavity, wherein a cross-sectional area of the sample channel is greater than a cross-sectional area of the liquid metering cavity, the cross-sectional area of the liquid metering cavity is greater than a cross-sectional area of the sample splitting channel, and a cross-sectional area of the first communication structure corresponding to the liquid metering cavity on a side adjacent to the liquid metering cavity is greater than or equal to the cross-sectional area of the sample channel.

A drug testing platform for simulating a lung environment includes a microfluidic chip and a stretching device. The microfluidic chip defines a microfluidic channel structure. The microfluidic channel structure includes a test unit, a cell shunt unit and a drug shunt unit. The stretching device is configured to stretch and contract the test unit in a manner of a reciprocal motion, thereby making degree of deformation of a plurality of test area of the test unit to be same during the reciprocal motion. The drug testing platform for simulating the lung environment according to the present disclosure may configure an environment that simulates a breathing motion of a human lung, and obtain multiple results within a single test.

The application provides a kind of light waveguide microfluid detection system based on CMOS image sensing, comprising: microfluid chip, spectral collection device and analysis device;Microfluid chip includes: light waveguide and micro flow channel, light waveguide is used to guide light into micro flow channel along horizontal direction;Microfluid chip further includes: CMOS image sensing layer, lower cladding layer, waveguide layer, upper cladding layer and flow channel cover plate are sequentially arranged from bottom to top, waveguide layer is formed by silicon nitride material at 25-150 DEG C deposition temperature, waveguide layer is used to form light waveguide;Micro flow channel penetrates upper cladding layer, waveguide layer and lower cladding layer from top to bottom to expose CMOS image sensing layer;Micro flow channel width is 10-100 μm.It has beneficial effects: low-temperature deposition of silicon nitride light waveguide with adjustable optical performance on CMOS image sensing layer and high molecular polymer material, without damaging CMOS image sensing layer, reducing the preparation work such as adjusting the collection light path in the experiment, improving the experimental efficiency;Improve the portability of detection system, greatly increase the application scene of system.

The application discloses a kind of visualization methods for microcosmic action mechanism of steam displacement heavy oil, which comprises: obtaining the porous structure information of rock sample;According to the structure information of rock sample, design pore network structure and make microfluidic chip;Build steam displacement heavy oil visualization implementation system, and adopt steam displacement heavy oil visualization implementation system to collect the phase distribution and temperature distribution of microfluidic chip in displacement process;Boundary extraction is carried out to experimental picture, and the dynamic change process of each phase in displacement process is obtained and the interaction relationship with temperature distribution change is obtained.The application designs pore network chip according to the pore structure information of rock sample, obtains the phase distribution and temperature distribution in displacement process, and is used to study the microcosmic phase distribution characteristics and temperature distribution characteristics of fluid in heavy oil reservoir reservoir, and then the microcosmic mechanism of reservoir fluid movement is explored, so as to provide a practical visualization method for the research of steam drive oil mechanism.

A microfluidic chip and a preparation method therefor, and a microfluidic system The microfluidic chip includes a first substrate a second substrate, and a gap control layer located between the first substrate and the second substrate wherein the gap control layer comprises an adhesive line and a plurality of gap beads of substantially the same size arranged at intervals in the adhesive line.

The invention provides a microfluidic device (100) for the formation of lipid bilayers, wherein the microfluidic device (100) comprises a first fluid inlet (111), a first fluid outlet (112), and a first microfluidic channel (110) configured to fluidically connect the first fluid inlet (111) and the first fluid outlet (112), wherein: (A) the first microfluidic channel (110) comprises a membrane formation section (200) having a membrane formation section axis (Emfs) of elongation, wherein the channel wall (115) at the membrane formation section (200) comprises a membrane formation wall section (215); (B) the membrane formation section (200) comprises a plurality of micropillars (210); (C) the plurality of micropillars (210) are configured in an n*m array parallel to the membrane formation section axis (Emfs) of elongation; wherein n is selected from the range of ≥1 and m is selected from the range of ≥2; wherein the n*m array is configured to define (i) n+1 elongated subchannels (110a,110b,110c...) within the first microfluidic channel (110) and (ii) per row of the n rows m-1 fluidic connection sections (105); wherein each of the plurality of micropillars (210) tapers in directions parallel to the membrane formation section axis (Emfs) of elongation towards respective adjacent micropillars (210); (D) the membrane formation wall section (215) is configured in a wave-like shape parallel to the membrane formation section axis (Emfs) of elongation.