1296 results about "Micro fluidic" patented technology

The invention discloses an intelligent defect detection system and a self-repairing method for a flexible base material RFID tag, and belongs to the technical field of Internet of Things electronic device manufacturing. According to the system, a three-dimensional dynamic scanning system is constructed by integrating a high-resolution image acquisition module, a multispectral sensor array and a mechanical arm motion platform, and surface and internal structure characteristics of a flexible substrate are captured in real time. A defect identification algorithm based on deep learning is combined with a multi-scale convolutional neural network and a transfer learning technology, precise classification of 12 types of defects such as microcracks, conductive layer fractures and base material deformation is realized, and the detection precision reaches 99.2%. A dual-mode self-repairing mechanism is put forward, specifically, nano-silver conductive colloid is injected through a microfluid channel for the defects of the conductive layer, and 3D structure reconstruction is achieved through a controllable temperature field; for substrate damage, a photoresponse shape memory polymer patch is adopted, and molecular-level bonding repair is achieved after ultraviolet light activation. According to the scheme, the detection efficiency is improved by more than 5 times, and the radio frequency performance of the tag is recovered to 98.7% of the initial value after self-repairing.

The invention discloses a high-precision liquid phase chip sample positioning and identification system, which belongs to the field of high-precision liquid phase chip sample positioning and identification, and comprises an active liquid phase sample positioning control module used for collecting a liquid flow state signal and adjusting the speed and position of an input sample in real time through an adjustable microfluid structure, fixed-point residence and directional conveying of samples in a chip channel are realized; the image recognition and feature position fine adjustment module is used for carrying out multi-modal image collection on an area where the sample is located, extracting spatial feature information of the target sample based on a deep learning recognition model, and carrying out fine adjustment correction on the position of the sample according to a feedback signal; by constructing an active liquid phase sample positioning and recognition system, high-precision directional control and spatial residence of a liquid sample in a chip channel are realized, the problems of sample drift and unstable recognition in a traditional passive conveying mode are effectively avoided, and the timeliness and accuracy of sample recognition are improved.

The invention relates to a design method and system for a monolithic integrated microfluid cooling channel of a power device, and the method comprises the steps: obtaining the size structure data, hot spot density data and heat source data of the power device, and carrying out the analysis of the microfluid cooling requirements, and obtaining the channel design requirements and heat source distribution information; performing microfluid channel network construction and surface insulation sealing treatment on a preset wide bandgap semiconductor substrate according to the channel design requirements to obtain a microfluid three-dimensional channel structure; performing power device active region layout according to the microfluid three-dimensional channel structure to obtain a device integrated structure; performing cooling simulation evaluation on the device integration structure according to the heat source distribution information to obtain a cooling evaluation result; and performing iterative verification optimization on the device integration structure according to the cooling evaluation result to obtain a target design scheme. The heat dissipation performance of the power device can be effectively improved, and the size of an external heat dissipation system is reduced.

Payment cards with activated information displays are described. In one embodiment, a payment card may include: a first layer; a second layer comprising an elastic material; an actuator; and an information layer between the first layer and the second layer, comprising: a plurality of information voids; and a microfluidic channel that communicates the plurality of information voids with the actuator. A fluid may be provided in the microfluidic channel such that when the actuator is depressed, fluid flows into the information voids making the information voids visible through the second layer, and when the actuator is released, the fluid flows out of the information voids making the information voids substantially invisible through the second layer.

The invention discloses an inertial micro-fluidic chip integrating cell focusing and sorting and a preparation method thereof. The inertial micro-fluidic chip comprises a cover plate layer, a runner layer and a substrate layer, a chip inlet, a first chip outlet and a second chip outlet are formed in the cover plate layer; the flow channel layer is provided with a sample liquid flow channel inlet, a first flow channel outlet and a second flow channel outlet, the chip inlet is communicated with the sample liquid flow channel inlet, the first chip outlet is communicated with the first flow channel outlet, and the second chip outlet is communicated with the second flow channel outlet. The flow channel layer is provided with a spiral cell sorting flow channel and a cell focusing flow channel, the front end of the spiral cell sorting flow channel is communicated with a sample liquid flow channel inlet, the tail end of the spiral cell sorting flow channel is communicated with the cell focusing flow channel and a first flow channel outlet, and the tail end of the cell focusing flow channel is communicated with a second flow channel outlet. Through a microfluid vortex focusing technology and a spiral flow channel inertia sorting technology, accurate sorting and focusing of cells are realized, and the problems that the focusing state of the sorted cells is influenced and the cell counting detection precision is low are effectively solved.

The invention discloses a sweat uric acid and glucose sensor based on a carbon nanotube (CNT) enhanced electrochemical platform, and aims to solve the sensitivity challenge of low-concentration biomarker detection in sweat. The enzyme is covalently immobilized on the CNT through an EDC / NHS chemical method, so that the catalytic efficiency, the electron transfer speed and the sensor stability are remarkably improved, and the ultralow detection lower limit of uric acid and glucose is realized. According to the device, uric acid, glucose and pH sensing functions are integrated on a single wearable platform, efficient flowing and rapid updating of sweat are ensured through a radiation symmetry microfluid design optimized through finite element analysis, and real-time biomarker monitoring is supported. The material innovation (such as CNT-enzyme complex) and fluid dynamics optimization of the invention overcome the key problem in the field of wearable biosensing, and provide an extensible solution for the next generation of non-invasive health monitoring.

The utility model relates to the technical field of cell cultivators, in particular to a pump-free perfusion micro-fluidic chip for multi-cell three-dimensional co-culture, which comprises a liquid storage layer, a liquid inlet and outlet layer, an upper channel layer, a lower channel layer and a packaging layer, realizes a one-way circulation perfusion dynamic co-culture system without a pumping system, reduces the occupied space of equipment, and is convenient to operate. Operation is simple, culture medium consumption is reduced, and cost is reduced; the separation of free NK cells and tumor infiltration NK cells is realized, and conditions are provided for researching molecular biological characteristics of different types of NK cells. The migration and interaction of the tumor spheres and the NK cells are observed in real time at the same time, so that the problem that a microfluid pump integrated by an external system is needed for separating free immune cells and tumor infiltration immune cells by existing equipment is solved.

The invention discloses a DLD chip and method based on high-flow-speed composite flow channel multi-target sorting. The chip comprises a sample inlet, a sheath fluid inlet, a rectification module and a two-stage deterministic lateral displacement DLD sorting module, the rectification module adopts a circular microcolumn array to optimize fluid distribution, and the first-stage module realizes primary sorting through a microfluid channel and is connected with the second-stage module; and the second-stage module is communicated with three sample outlets after being accurately sorted through a microfluid channel II. The chip is made of a PDMS material through a soft photoetching process, and the two stages of sorting modules are designed to be of a rectangular structure connected end to end and are respectively composed of elliptical micro-columns with opposite deflection angles. Under the high-flow-rate working condition, stable separation of cells can be kept, blockage is not prone to occurring, the good sorting effect on various cells of different sizes is achieved, the flow channel layout is compact, the chip manufacturing process is simplified, and the chip is suitable for the sorting scene with the high-flux requirement.

The invention belongs to the technical field of construction of super-hydrophobic surfaces, and particularly relates to a preparation method of a super-hydrophobic coating with a multistage micro-nano structure. Comprising the following steps: pretreating a substrate; slowly adding the F-SiO2 nanoparticle dispersion liquid into the fluorine-containing glue solution under the synergistic treatment of ultrasonic and magnetic stirring to obtain an F-SiO2 / fluorine-containing glue composite solution; the method comprises the following steps: determining the actual glue coating amount based on the theoretical glue demand amount of a PDMS template, and coating the F-SiO2 / fluorine-containing glue composite solution on the surface of a substrate through spin coating to form a wet film; coining the wet film by using a PDMS template, and stripping the template after ultraviolet curing to obtain the super-hydrophobic coating with the multistage micro-nano structure. Based on an imprint method with insufficient glue amount, synchronous construction of a micro / nano dual-scale structure is achieved by accurately controlling the ratio of the volume of the coating liquid to the volume of the pore of the template and combining the curing shrinkage characteristic of the polymer, and the method has application potential in the fields of anti-icing, microfluid devices, marine antifouling and the like.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

Some methods of performing an assay comprise culturing target cells in one or more channels of one or more microfluidics chips, where each of the channel(s) can have a volume that is less than or equal to 100 microliters (μL). For each of one or more test liquids that each comprise a therapeutic reagent, the test liquid can flow over the cultured target cells in at least one of the channel(s) and, while the test liquid flows over the cultured target cells, data indicative of an interaction between the target cells over which the test liquid flows and the therapeutic reagent of the test liquid can be captured.

The utility model provides a microfluid sample detection device which comprises a fluid inlet, a microfluid channel, a fluid outlet, a reaction area, a liquid collecting area, a pump body and a valve body, the valve body comprises a substrate, a double-sided colloid and a flexible diaphragm, a cavity is formed between the upper surface of the plastic substrate and the lower surface of the flexible diaphragm, the cavity is opened when the flexible diaphragm is loosened, and the cavity is closed when the flexible diaphragm is tensioned; the valve body comprises a first valve body, a second valve body, a third valve body and a fourth valve body, the second valve body and the third valve body are arranged between the pump body and the microfluid channel and located on the downstream portion of the first valve body, and the working states of the third valve body and the fourth valve body are opposite. Moreover, the parallel scheme further comprises a conductive system composed of electrodes, the conductive system at least comprises two electrodes, and the electrode sensing ends are located at the two ends of the reaction area respectively. The microfluidic sample detection device is loaded in a detection device to form a microfluidic sample detection system.

The invention provides an oil aliasing particle detection device based on a multi-frequency eddy current effect, which adopts a multi-mode electromagnetic sensing architecture, integrates three groups of orthogonally arranged solenoid coil detection units in a single detection channel, and respectively loads low-frequency (10-100kHz), intermediate-frequency (100kHz-1MHz) and high-frequency (1-10MHz) excitation signals with different characteristics, so as to detect the aliasing particles in the oil. Based on frequency domain response characteristic differences presented by different metal particles in high, medium and low frequency eddy current fields, a particle material-size-space distribution frequency domain characteristic matrix is constructed through multi-physics field coupling modeling, and synchronous identification and orthogonal decoupling of ferromagnetic particles and non-ferromagnetic particles are realized. According to the invention, the eddy current effect difference characteristics of metal / non-metal particles under excitation of different frequencies are utilized, and a signal decoupling algorithm is combined to realize accurate distinguishing of aliasing particles, so that the reliability of high-flux oil detection is remarkably improved; and a key technical support is provided for engineering application of a microfluid chip technology in the fields of engine wear monitoring, hydraulic system fault early warning and the like.

The invention discloses a biaxial silicon micro-accelerometer based on a three-degree-of-freedom weak coupling resonator, and belongs to the technical field of micro-electro-mechanical systems and microfluid measurement. Comprising a glass base and a silicon micromechanical sensor, the center of the glass base is provided with a protruding common anchor point, the silicon micromechanical sensor is connected to the glass base through the common anchor point, and signal electrodes for transmitting signals are distributed on the glass base; the silicon micromechanical sensor comprises a stress base and resonance modules; the stress base is mounted on the glass base, and the resonance modules arranged in a Y shape are arranged on the stress base; the Y-shaped head of the resonance module is provided with a pair of resonators which are perpendicular to each other and are used for detecting acceleration in the horizontal direction and acceleration in the vertical direction respectively. The stability and the measurement accuracy of the biaxial accelerometer are further improved by arranging a resonator arrangement mode of a stable structure and cooperating with the mechanical stable design of the amplification unit and the safety structure.

The present application provides a microfluidic device, comprising at least one microfluidic channel unit, each microfluidic channel unit comprising a microfluidic channel configured to contain a fluid and comprising a plurality of independent compartments. Each microfluidic channel unit comprises a first side wall and a second side wall opposite and spaced apart from the first side wall, a plurality of first extensions formed on the first side wall extending toward the second side wall without abutting the second side wall, a plurality of second extensions formed on the second side wall extending toward the first side wall without abutting the first side wall, the plurality of first extensions and the plurality of second extensions being alternately arranged in the microfluidic channel. A compartment is formed in at least one first extension or at least one second extension. The compartment has an opening positioned such that the fluid does not directly flow into each compartment. The microfluidic device of the present application has a stable isolated environment to facilitate various reactions and prevent micro-objects in the compartments from escaping.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention discloses a preparation method and equipment of three-chamber eccentric parallel structure nanofibers. The preparation method comprises the following steps: simultaneously carrying out high-voltage electrostatic spinning on three spinning solutions; the two spinning solutions are respectively divided into microfluids to be led out through an array formed by two sets of series metal capillary tubes; the other spinning solution is divided into microfluids to be led out through a plurality of bottom through holes of the insulating container; the first series of metal capillary tubes are inserted into the second series of metal capillary tubes to form a series of metal capillary eccentric sleeves; the series of metal capillary eccentric sleeves penetrate through the insulating container and extend out of one side of the interior of the bottom through hole to form a combined eccentric outlet of three strands of spinning solutions; three spinning solutions are guided into a high-voltage electrostatic field in an eccentric parallel mode through the combined eccentric outlet, and the three-chamber eccentric parallel structure nanofiber is formed through single-step direct stretching. According to the method, the capability of preparing the three-chamber eccentric parallel structure characteristic nanofiber with a complete structure in a single-step, batch and large-scale manner is endowed.

The utility model provides a microfluid sample detection device and a microfluid sample detection system. The microfluid sample detection device comprises a fluid inlet, a microfluid channel, a fluid outlet, a reaction area, a liquid collecting area, a pump body, a valve body and a lock body, the valve body comprises a substrate, a double-sided colloid and a flexible diaphragm, a cavity is formed between the upper surface of the plastic substrate and the lower surface of the flexible diaphragm, the cavity is opened when the flexible diaphragm is loosened, and the cavity is closed when the flexible diaphragm is tensioned; the valve body comprises a first valve body and a second valve body, the second valve body is arranged on the downstream of the first valve body, and the working states of the first valve body and the second valve body are opposite; the lock body is arranged on the downstream of the second valve body. Moreover, the parallel scheme further comprises a conductive system composed of electrodes, the conductive system at least comprises two electrodes, and the electrode sensing ends are located at the two ends of the reaction area respectively. The microfluidic sample detection device is loaded in a detection device to form a microfluidic sample detection system.

A semiconductor structure and a microfluidic system comprising the semiconductor structure are disclosed. The semiconductor structure comprises a thermoelement layer. The thermoelement layer comprises p- and n-type thermoelements. These thermoelements form regions wherein respective region is associated with a specific temperature range, where achieving the specific temperature range is based on an electron or hole current flowing through the thermoelements. The semiconductor structure forms part of the microfluidic system comprising a microfluidic channel having a meander extension across regions having different temperature ranges. This allows a fluid flowing in the microfluidic channel being exposable to cyclic temperature variations.

A method of culturing cells, the method including providing a microfluidic device including a substrate including an array of grooves that are substantially parallel; introducing cells into one or more of the grooves; providing a flow of culture medium over the groove array in a first flow direction that is across the grooves; culturing the cells under conditions to support viability, growth or differentiation, optionally wherein: (i) the cells are introduced into one or more grooves by flow over the groove array in the first flow direction; and / or (ii) the flow of culture medium is provided at a flow rate equivalent to 0.2-5 mL per million cells per day; and / or (iii) the cells are cultured for 1-14 days.

Provided herein are methods for replenishing dissolved gas to aqueous droplets on a digital microfluidic device. Provided herein are methods of cell-free protein synthesis, optimized cell-free protein synthesis (CFPS) reagents, and methods for optimizing CFPS reagents to increase protein expression yield. The method is suitable for protein expression on microfluidic devices having hydrophobic surfaces by circulating a fill fluid to introduce additional oxygen.

The invention discloses a heat dissipation enhanced semiconductor packaging method, which comprises the following steps of: performing deep silicon etching to form a vertical through hole; insulation and metallization are carried out; thinning the wafer; performing hybrid bonding and stacking of multiple layers of chips; a heat dissipation design is adopted to form a vertical heat dissipation path; a three-dimensional thermal through hole network: adding pure copper thermal through holes in a non-functional area; performing microfluid cooling integration; molding and packaging; and thermal performance verification: verifying that the junction temperature can be controlled within 85 DEG C through infrared thermal imaging and finite element simulation. The TSV with the depth-to-width ratio of 10: 1 can be realized by adopting the Bosch process; a three-dimensional integrated TSV array supporting HBM and other high-speed memories is embedded into diamond or graphene heat dissipation columns, a low-heat-resistance vertical heat dissipation path is formed by combining a three-dimensional pure copper heat through hole network, and local hot spots are effectively dispersed; the high-thermal-conductivity epoxy molding compound and the embedded copper block enhance the overall mechanical strength and thermal diffusivity, so that the product can dissipate heat efficiently, and the overall area is not easy to influence.

An example one-time-open microfluidic valve can include an inlet microfluidic channel in a first elevation plane and an outlet channel in a second elevation plane. An upstream end of the outlet channel can overlap a downstream end of the inlet microfluidic channel. A capillary nozzle opening can connect the inlet microfluidic channel to the outlet channel at the overlapping ends. The capillary nozzle opening can have an outlet width that is smaller than a width of the outlet channel. A fluid actuator can be positioned to eject a fluid in the inlet microfluidic channel through the capillary nozzle opening into the outlet channel.

The utility model discloses a microfluid device and a system for researching abnormal migration of solute of a fractured porous medium system. The microfluid device for studying abnormal migration of the solute of the fractured porous medium system comprises a chip and a substrate, wherein the lower surface of the chip is bonded with the upper surface of the substrate; a crack and a porous medium area distributed around the crack are patterned on the lower surface of the chip; the porous medium area comprises a plurality of cylinders, gaps are reserved among the cylinders, and flow channels communicated with the cracks are formed; and a part or all of the cylinders in the porous medium area are provided with notches serving as blind ends. The microfluid system for studying abnormal migration of the solute of the fractured porous medium system comprises a fluid injection device, the microfluid device and a fluid collection device which are connected in sequence.

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

This disclosure includes description of various micro-cooling devices, systems, and methods. An example micro-cooling device includes a first substrate, a second substrate coupled to the first substrate, and a microfluidic channel formed between the first substrate and the second substrate. The example micro-cooling device further includes a fluid contained within the microfluidic channel, and a set of one or more piezoelectric actuators positioned adjacent to the microfluidic channel and configured to govern flow of the fluid within the microfluidic channel. An example micro-cooling system includes one or more of the micro-cooling devices as well as one or more sensors and control circuitry coupled to the one or more sensors and configured to govern operation of the micro-cooling device(s) accordingly.

The invention relates to an extraction method for strengthening low-concentration rare earth elements and equipment for implementing the extraction method. The extraction method is a continuous extraction process based on the combination of a microfluid technology and a solvent extraction technology, and efficient enrichment and recovery of low-concentration rare earth elements are realized through combined use of a gas-liquid-liquid three-phase system and an extraction agent. The extraction method provided by the invention has the advantages of high mass transfer efficiency, simple operation, recyclable extraction agent, high extraction efficiency, good stability and the like, and is suitable for enrichment and recovery of low-concentration rare earth elements on an industrial scale under the condition of a large phase ratio (greater than 50).

The utility model relates to the technical field of microfluidics, and discloses a multi-stage micro-fluidic chip, which at least comprises a first-stage collision fluid channel and a second-stage collision fluid channel, and a mixing channel of the upper-stage fluid channel is arranged as a premixing fluid channel of the lower-stage fluid channel; the first-stage collision fluid channel comprises a first premixed fluid channel and a second premixed fluid channel, a first-stage fluid collision channel is arranged between the first premixed fluid channel and the second premixed fluid channel, and the first-stage fluid collision channel enters the second-stage premixed fluid channel after collision; a second-stage fluid collision channel is arranged between the third premixing fluid channel and the fourth premixing fluid channel, and the second-stage fluid collision channel enters the mixing channel after collision. The multi-stage micro-fluidic chip has the advantages of high mixing efficiency, low shear damage, easiness in integrated packaging and batch manufacturing and the like, and the micro-fluid mixing performance and the system practicability are remarkably improved.

The invention belongs to the technical field of medical treatment, and discloses a preparation process for accurately regulating and controlling the component proportion of a blood product. The process comprises the following steps: blood collection and pretreatment; blood components are accurately separated; performing real-time component detection; intelligent regulation and control and closed-loop feedback are realized; and optimizing a finished product and monitoring quality. According to the preparation process capable of accurately regulating and controlling the component proportion of the blood product, by integrating AI intelligent control, microfluid separation, real-time sensor detection, dynamic feedback regulation and control and other technologies, high-precision, automatic and intelligent blending of blood components is achieved. The AI intelligent optimization algorithm is adopted, the composition proportion is dynamically adjusted, and the precision error of a finished product is reduced to be within + / -1% from traditional + / -5%. Intelligent feedback closed-loop control avoids deviation caused by human intervention, and the consistency is improved. In the traditional centrifugal separation, parameters need to be manually adjusted, and the separation efficiency is low, while the micro-fluid centrifugal technology is adopted in the method, efficient separation can be completed within 5-10 minutes, and the overall productivity is improved by 30%-50%.

The invention relates to a consumable device for sweat-based monitoring, the consumable device comprising a proximal part configured to contact with a user, and further comprising: a) a sweat induction means on the proximal part, comprising: i. a sweat promoter configured to contact the skin of a user, and ii. a microfluidic structure; and b) a sweat interrogation means, located distally to the sweat induction means and comprising: i. a sample area; and ii. a passive fluid pump configured to induce the flow of the liquid through the sample area. The microfluidic structure is configured to convey fluid from the sweat promoter to the sample area. The invention also relates to a sweat-monitoring system comprising: a consumable device as above and a sweat-based monitoring device, the sweat-based monitoring device comprising sensing means and processing means.