450 results about "Pathogen detection" patented technology

The invention discloses a hot air type qPCR instrument, a multi-channel detection optical system and a detection method, and relates to the field of molecular biological detection. The problems that an optical detection system adopted by an existing qPCR instrument mostly depends on a single-channel or double-channel design, sample hole sites need to be switched one by one and detected in a time-sharing mode in the detection process, namely, the efficiency is low in the face of a high-throughput requirement scene are solved, and the system combines multiple LED light sources with an optical filter to generate excitation wavelengths to form a light source module; after the multi-stage dichroscope is utilized to form a light combination module, the light combination module is transmitted to a reaction hole through one branch of the forked optical fiber to excite fluorescent substances, a fluorescent signal is collected through a common end of the forked optical fiber, the other branch of the forked optical fiber outputs a fluorescent signal, and the fluorescent signal is split by the reflection grating to form a light splitting module. And the array CMOS is combined with a spectrum unmixing algorithm to form a detection module for synchronous receiving, so that multi-channel simultaneous high-sensitivity detection is realized. The method is also suitable for the field of multi-pathogen detection and gene expression analysis.

The invention provides a nucleic acid composition for respiratory tract pathogen detection and application thereof, and relates to the technical field of biology. The invention provides a nucleic acid detection composition which has high specificity and can be used for detecting various respiratory pathogens. The composition can be used for detecting influenza B virus, influenza A virus H1N1, influenza A virus H3N2, novel coronavirus N gene, novel coronavirus O gene, respiratory syncytial virus, I-type parainfluenza virus, II-type parainfluenza virus, III-type parainfluenza virus, IV-type parainfluenza virus, rhinovirus, adenovirus, haemophilus influenzae, and the like. The kit has 15 pathogens including streptococcus pneumoniae and staphylococcus aureus, the lower detection limit can be as low as 20 copies / reaction, and the kit plays an important role in rapid detection of respiratory tract pathogens.

The invention relates to the technical field of germ detection, in particular to a method and a device for detecting response of corn to setosphaeria turcica infection. The method comprises the following steps: acquiring initial spectral reflectivity of corn leaves and environmental parameters of an environment where the corn leaves are located; the environment parameters comprise temperature, humidity and illumination; according to the growth characteristics of the corn leaves in different growth stages, extracting the initial spectral reflectance to obtain a key spectral reflectance; extracting the spatial-spectral characteristics of the key spectral reflectivity, and extracting the time sequence correlation characteristics of the environmental parameters and the key spectral reflectivity; generating a disease sensitive feature vector according to the space-spectrum feature and the time sequence correlation feature; according to the disease sensitive feature vector, determining the infection detection result of the corn leaves responding to the setosphaeria turcica.

A pathogen detection system and method are disclosed. The pathogen detection system includes a pathogen sensor comprising a plasmonically active pathogen sensing area that comprises a hybrid structure of electrically conductive surface and metal nanofractals, forming a surface enhanced Raman spectroscopic (SERS) substrate and a controller comprising a memory storing one or more trained machine learning algorithms, that perform label-free identification and differentiation of a pathogen or the pathogen and a pathogen mutation based on acquired Raman spectra.

An intelligent cooling system for dynamic monitoring, prediction and optimization of storage conditions in real time, consisting of: a refrigerator with heat-insulated walls; a sensor array in the refrigerator comprising temperature sensors, humidity sensors, pressure sensors, and pathogen detection biosensors, the sensor array being configured to continuously monitor internal environmental parameters in the refrigerator and external environmental conditions; a control dashboard configured to display real-time environmental data and enable remote monitoring and manual adjustment of the environment in the refrigerator; an Internet of Things (IoT) communication module configured to transmit the monitored internal and external environmental data to the control dashboard; a proportional-integral-derivative (PID) controller operatively connected to the IoT communication module and configured to analyze the monitored data and calculate correction factors to maintain optimal environmental conditions; a plurality of actuators within the refrigerator, the plurality of actuators comprising a temperature actuator, a humidity actuator, and a pressure actuator, the actuators being configured to independently adjust temperature, humidity, and pressure within the refrigerator based on the control instructions; a processor configured to execute machine learning algorithms to analyze historical and real-time sensor data, predict optimal environmental conditions based on the analyzed data, and generate control instructions for the actuators based on the predicted optimal environmental conditions and the calculated correction factors; and a solar power supply configured to provide energy for operating the system.

The invention belongs to the technical field of detection of pathogens of canine babesia, and discloses a primer probe composition, a detection kit and a detection method for distinguishing canine babesia and babesia gibsoni. The primer probe composition comprises an upstream primer gimbal-F of which the sequence is as shown in SEQ ID NO.1, a downstream primer gimbal-R of which the sequence is as shown in SEQ ID NO.2, and a fluorescent probe gimbal-P of which the sequence is as shown in SEQ ID NO.3; the kit comprises an upstream primer Kiba-F with a sequence as shown in SEQ ID NO.4, a downstream primer Kiba-R with a sequence as shown in SEQ ID NO.5, and a fluorescent probe Kiba-P with a sequence as shown in SEQ ID NO.6. The detection kit comprises the primer probe composition. The detection method comprises the steps of sample treatment, fluorescent quantitative amplification and detection result analysis. According to the detection method, the babesia gibsoni and the babesia canis in a sample can be accurately detected at the same time and effectively distinguished, and an accurate medication scheme is provided for dogs infected with the babesia gibsoni.

The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses, a detection method and application, and particularly belongs to the technical field of porcine pathogen detection products. The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses. The primer probe group comprises universal forward primers PRRSV1-F and PRRSV2-F for detection, reverse primers PRRSV1-R and PRRSV2-R for detection and a universal probe PRRSV-P. A target gene detected by the primer probe group is a 3'non-coding region gene (3 'UTR) of a porcine reproductive and respiratory syndrome virus genome, and the primer probe group can realize efficient detection of American strains and European strains of the porcine reproductive and respiratory syndrome virus, and is simple and convenient in detection, high in sensitivity, good in specificity, high in speed, low in false positive and capable of realizing high-throughput detection.

The invention relates to the technical field of pathogen detection, relates to a POCT (point-of-care testing) rapid nucleic acid detection system, and in particular relates to a double-stranded probe-assisted loop-mediated isothermal amplification system for detecting brucella. According to the system, an amplification primer and a detection double-stranded probe are designed aiming at a Brucella specific gene bcsp31, and loop-mediated isothermal amplification (LAMP) and a double-stranded probe (TP) based on foot-mediated strand displacement are integrated to form a TP-LAMP unit of the scheme. The amplification result can be monitored in real time through a PCR luminoscope, and can also be visually identified through a nano biosensor (AuNPs-LFB). The method is rapid, sensitive, specific and easy to use, can solve the technical problems of non-ideal detection speed, specificity and sensitivity of a brucella POCT detection technology in the prior art, can be used as a valuable brucella rapid identification tool, and has ideal application and popularization prospects.

The invention relates to the technical field of preventive medicine, and discloses a mouse-borne disease intelligent monitoring and early warning device based on multi-source data fusion, which comprises an environment sensor module, a mouse activity monitoring module, a pathogen detection module, a human case data acquisition module, a data fusion processing unit and an early warning and decision support unit, the data fusion processing unit is used for integrating and analyzing multi-source heterogeneous data from the above modules, and the early warning and decision support unit generates early warning information and prevention and control suggestions based on an analysis result. According to the method, the environment data, the mouse activity data, the pathogen detection data and the human case data are integrated, a multi-dimensional monitoring system is constructed, space-time correlation analysis is performed in combination with a deep learning algorithm, the prediction accuracy of disease outbreak is remarkably improved, the mouse recognition accuracy is high, and the pathogen detection specificity is excellent.

The invention discloses a low-abundance pathogen detection method and system based on metagenome sequencing data, and belongs to the technical field of bioinformatics. The system comprises a species specificity sequence screening module (BactAlert2SSRs), a reference genome database construction module (BactAlert2Lib), a sequence alignment reference genome module (BactAlert2Map), a sequence alignment result analysis module (BactAlert2ID), and an SSRs posterior probability correction module (BactAlert2Rec). The detection system realizes efficient, accurate and reliable detection of low-abundance pathogens, and is suitable for analyzing third-generation metagenome sequencing data.

The invention discloses a hydrogel chip as well as a preparation method and application thereof. The hydrogel chip comprises a chip shell, a hydrogel array and a sample flow channel, wherein the hydrogel array and the sample flow channel are arranged in the chip shell. The hydrogel chip provided by the invention is simple in manufacturing process, convenient to use and low in preparation cost, can realize ultra-multiple pathogen detection, can perform PCR, melting curve analysis and real-time quantitative PCR, can flexibly set detection items according to actual requirements, is high in expandability, can realize multi-target rapid detection, is simple to operate, and is suitable for large-scale popularization and application. And the hydrogel chip is a totally closed reaction system, so that pollution can be effectively prevented.

The invention discloses an anti-Ebola virus VP40 protein nano antibody and an application of the anti-Ebola virus VP40 protein nano antibody in Ebola virus detection. The anti-Ebola virus VP40 protein nano antibody comprises a 3E07 antibody and a 3F06 antibody. The anti-Ebola virus VP40 protein nano antibody prepared by the invention has the characteristics of high sensitivity, wide binding spectrum and the like. A double-antibody sandwich enzyme-linked immunosorbent assay method constructed by the 3E07 and 3F06 antibodies can be used for efficiently detecting VP40 proteins and virus-like particles of various subtype Ebola viruses such as Zaire, Sudan, Bandong and the like, and the detection sensitivity for the VP40 proteins of the Zaire type Ebola viruses can reach 0.039 ng / mL; the antibody combination has a wide application prospect in diagnosis and pathogen detection of Ebola virus diseases.

The invention discloses a method and system for detecting respiratory tract pathogens, pathogen drug-resistant genes and pathogen virulence genes, and relates to the technical field of gene detection.The method comprises the steps that a respiratory tract sample of a patient is collected, DNA and RNA are extracted, and total nucleic acid is obtained; carrying out first-round PCR amplification on the total nucleic acid, purifying a first-round PCR product, carrying out second-round PCR amplification, and sorting the product to obtain a library building product; performing high-throughput sequencing on the library building product to obtain sequencing data, and detecting pathogens, drug-resistant genes and virulence genes; according to the method and system for detecting the respiratory tract pathogens, the pathogen drug-resistant genes and the pathogen virulence genes, by optimizing library preparation and direct library building of nucleic acid, the process is simplified, and efficiency and accuracy are improved; the balance reagent design guarantees library quality; the kit adapts to pathogen detection requirements in Yunnan and the like, provides efficient diagnosis, shortens detection time, improves treatment effect, regularly updates pathogen information, and keeps technical frontier.

The invention belongs to the technical field of biological detection, and particularly discloses a targeted sequencing primer group and a kit for detecting common pathogens of children and drug-resistant genes thereof. The primer group comprises 145 primer pairs of common children pathogen primer groups and 67 primer pairs of drug-resistant gene primer groups of the common children pathogen primer groups. According to the kit provided by the invention, nucleic acid in a clinical sample is directly extracted, 212 pairs of specific primers are adopted to realize multi-targeted amplification of a pathogen target area, a high-throughput sequencing platform is adopted to accurately identify pathogens and drug-resistant mutant genes thereof, 72 common children pathogens and drug-resistant genes thereof can be detected at the same time, and the kit has a wide application prospect. More accurate diagnosis information is provided for clinic, a key technical support is provided for early-stage accurate treatment of children infectious diseases, and the technical problems that in the prior art, in children pathogen detection, the detection speed is low, the number of missed detection is large, and the drug-resistant blind area is large are solved.

Disclosed herein are methods, compositions, and kits related to the identification and / or quantification of target molecules. Furthermore, the disclosure relates to compositions, kits, and methods for detecting and quantifying molecular targets, and especially for detecting and quantifying DNA and RNA The apparatuses and methods. may find use in a variety of applications including prenatal testing detection of pathogens and screening of cancer.

The present invention relates to a primer set and a detection method for simultaneously qualitatively and quantitatively detecting five pathogens and their application, belonging to the technical field of pathogen detection. The specific sequence of the detection primer set is shown in SEQ ID NO.1-10. The present invention also provides a kit and a detection method containing the primer set. The detection primer set and the detection method of the present invention have strong specificity, simple and convenient operation, short detection time, high sensitivity, and can simultaneously qualitatively and quantitatively detect EHP, DIV1, IHHNV, WSSV and V AHPND Five pathogens.

The invention relates to the technical field of silkworm disease prevention and control, in particular to a silkworm disease prevention and control method and system for predicting pathogen trend based on artificial intelligence. The method comprises the following steps: screening pathogen monitoring sites from silkworm industrial sites, and collecting samples to be detected from the pathogen monitoring sites based on a preset sampling rule; a detection result and corresponding weather data during pathogen detection are stored in a pathogen database; setting a to-be-predicted pathogen type and initial prediction time, and starting from the initial prediction time, obtaining weather forecast data of multiple days in the future; inputting the weather forecast data into the pathogen prediction model to obtain pathogen concentrations of pathogen types in multiple days in the future, and recording the pathogen concentrations as a first prediction content set; and grading the first predicted content set, and determining early warning and prevention and control measures according to a grading result. A pathogen prediction model based on artificial intelligence is used for receiving weather forecast data, the proliferation result of pathogens in multiple days in the future is predicted, prevention and control measures can be laid out in advance on silkworm industry points, and the risk of pathogen outbreak is reduced.

The invention belongs to the field of pathogen detection, and particularly relates to a rapid detection kit for detecting mycoplasma pneumoniae and drug-resistant genes, the kit comprises: a mycoplasma pneumoniae upstream primer, a mycoplasma pneumoniae downstream primer, and a probe for detecting mycoplasma pneumoniae; the kit comprises an upstream primer and a downstream primer of the drug-resistant mutant gene at the 23S rRNA A2063G site of the mycoplasma pneumoniae, and a probe for detecting the drug-resistant mutant gene at the 23S rRNA A2063G site of the mycoplasma pneumoniae. The kit provided by the invention is shorter in actual amplification time and better in detection effect, can provide more accurate and more reference value detection results for clinicians, can complete detection under general instruments in the market, and is better in general applicability and wider in application range.

The invention relates to the field of pathogen detection, in particular to a primer probe group, a gene chip and a kit for detecting seven waterfowl infectious disease pathogens and application. The primer probe group provided by the invention can be used for simultaneously detecting the goose parvovirus, the duck viral enteritis virus, the muscovy duck parvovirus, the duck hepatitis A virus type 1, the duck hepatitis A virus type 3, the duck tembusu virus and the novel duck reovirus, and is strong in specificity and high in sensitivity. Furthermore, a gene chip prepared by using the primer probe group provided by the invention not only has strong specificity and high sensitivity (1.0 * 100 copies / [mu] L), but also has good repeatability and stability, can be stably stored within at least 180 days, has sensitivity superior to that of a standard fluorescent quantitative PCR method, can rapidly and efficiently detect main epidemic disease pathogens of waterfowl together, and has good application prospects. The method can be used for epidemiological investigation and disease diagnosis of waterfowls.

The invention relates to the technical field of molecular diagnosis, in particular to a multiplex nucleic acid detection primer probe combination and application thereof. A corresponding primer probe combination is designed for researching 10 common respiratory tract pathogens such as new coronavirus, influenza A virus, influenza B virus, adenovirus, rhinovirus, respiratory syncytial virus and human parainfluenza virus types 1, 2, 3 and 4, a respiratory tract infection multiple pathogen detection system is established, a multiple RT-qPCR system is optimized, and the kit is used for detecting the respiratory tract infection multiple pathogens. The optimized system is applied to a clinical throat swab sample for verification, and the multiple pathogen nucleic acid detection kit is researched and developed and used for detecting viral respiratory tract infection. According to the invention, multiple pathogens are detected by adopting a multiple RT-qPCR method, so that the detection time can be shortened, the detection sensitivity and specificity are improved, the detection cost is reduced, and the kit has important clinical significance on rapid diagnosis of respiratory tract multiple pathogen infection.

The present invention "integrated POCT test kit, test tube and test system for detecting 15 diarrhea pathogens" belongs to the field of biomedical testing. The test kit includes: fecal sample pretreatment reagents and pathogen detection reagents; the fecal sample pretreatment reagents include: 1~4M guanidine salt, 20~200mM sodium citrate, 1~10% SDS by volume, 2~10% polidocanol by volume, 25~50% isopropanol by volume, etc., 10~30mg / mL nanocarbon, 10~30mg / mL zeolite powder; pathogen detection reagents include system A, system B, system C, and system D reagents. The present invention also provides a test tube and a test system based on the test kit. The present invention can not only detect 15 pathogens at the same time, but also achieve the purpose of integrated POCT detection with good repeatability of test results, large test sample size, high sensitivity, good specificity, good test timeliness, and sample-in and result-out.

The invention provides a primer design method of a multiplex PCR targeted sequencing technology, which designs a primer with high specificity and wide genome coverage rate by combining multiple indexes with fine screening of high-quality species genomes, effectively optimizes the formation of dimers of a primer system, and improves the specificity and efficiency of detection. According to the technology, the high specificity is guaranteed, meanwhile, the performance of a multiple primer system is remarkably improved, the common dimer problem is optimized, and the high efficiency of multiple pathogen detection is guaranteed.

The invention relates to the technical field of nucleic acid on-site rapid visual detection, in particular to a one-pot rapid nucleic acid detection system based on an SfaTnpB combined isothermal amplification technology and application of the one-pot rapid nucleic acid detection system. According to the invention, an RAA isothermal amplification technology and an LAMP isothermal amplification technology are respectively combined with SfaTnpB nuclease, and finally, an RAA-SfaTnpB one-pot method system and an LAMP-SfaTnpB one-pot method system are successfully established. The one-pot system established by the invention has the advantages of high sensitivity, high specificity, fast reaction, strong accuracy and no need of complex equipment, the detection result can be visually interpreted by naked eyes, the system has high convenience and practicability, the adaptability and practicability of the system are further improved, and the rapid detection requirements in various scenes can be flexibly met. The system has a wide application prospect in the fields of rapid pathogen detection, genotype detection, early diagnosis of infectious diseases and the like.

The invention discloses a pathogen detection primer group, a kit and application in eye infection pathogen detection, the detection primer group comprises 162 pairs of primers for 54 pathogenic microorganisms, the interference competition among the primers is small, the 54 pathogenic microorganisms can be detected at the same time by randomly selecting one pair of primers for each pathogenic microorganism, and the detection efficiency is high. When all 162 pairs of primers are selected, the sensitivity and specificity of species identification can be improved, the specific and high-sensitivity detection of bacteria, fungi, viruses, parasites and accurately identified species can be met, and the requirements of clinical accurate diagnosis are met.

The invention provides a pathogen detection method based on targeted high-throughput sequencing. The pathogen detection method comprises the following steps: S1, acquiring NGS original sequencing data of a pathogen sample to be detected and a negative control sample; s2, removing low-quality data from the obtained original sequencing data, then performing host sequence filtering, and finally performing plasmid sequence filtering to obtain high-quality sequencing Read; s3, comparing the pathogen reference genomes to obtain specific Read data; s4, performing classification according to a comparison result, and calculating the specific Read number and the RPM value of each pathogen; s5, constructing a microbial reference genome database, and verifying the species specificity of the specific Read in the NT database; and S6, grading pathogen detection signals in the to-be-detected sample according to the RPM value and the NTRPM value to obtain a result. The method provided by the invention can effectively reduce false positive of a detection result and interference of background signals, the detection result is accurate, and the method can be well applied to the fields of disease diagnosis, epidemiological investigation, public health prevention and control and the like.

The invention discloses a primer combination and a kit for detecting intestinal infection pathogens and application. The primer combination for detecting pathogens disclosed by the invention comprises or consists of the following components: 28 amplification primers and 14 quality probe extension primers. 13 pathogens can be detected at a time, the detection cost is low, the operation is simple and convenient, and the pathogen detection efficiency is remarkably improved.

The invention relates to the technical field of pathogen detection, and discloses a respiratory system infection viroid sample detection device, which comprises a detection cylinder, a detection card is arranged in the detection cylinder, a one-way air inlet blowing nozzle is arranged at the port of the detection cylinder, and blown air is guided to be stored in an air cavity in the detection cylinder; the detection card comprises a gas production area, the surface is horizontally unfolded opposite to the gas outlet end of the blowing nozzle, and a gap in contact with the gas cavity is reserved in the gas production area; the transparent window is arranged outside the detection cylinder, and a display area is arranged in the middle of the detection card. The handheld portable blowing detection tube is adopted, breath containing droplets is guided to be intensively blown to a detection card sample introduction area, the structure is simple, sampling and detection are efficiently integrated, the infection risk in the use process is greatly reduced, medical staff are not needed for detection, a large amount of manpower and material resources are saved, and the detection efficiency is improved. The method is especially suitable for rapid screening of people closely contacted with a large number of suspected cases and definite cases, and the pressure of detection and diagnosis work can be effectively relieved.

The invention provides a construction method of a bacterial pathogen detection model based on nanopore adaptive sampling and deep learning, and a method, equipment, a medium and a program product for detecting bacterial pathogens by applying the model, and relates to the technical field of nanopore sequencing. The bacterial pathogen detection method comprises the following steps: obtaining a water sample to be detected; and inputting a to-be-detected water sample into the detection model constructed in the invention to obtain a detection result of the to-be-detected water sample. The sensitivity and the accuracy of pathogen detection are obviously improved, and the improvement on prevention and control of water-borne diseases is facilitated.

This invention discloses primer compositions, detection products, and applications for detecting 17 pathogens, relating to the field of biotechnology. A primer composition for detecting 17 pathogens includes 17 pairs of amplification primers and 17 extension probes, as shown in SEQ ID NO: 1-51, respectively. This invention also provides detection products comprising the above primer compositions and their application in simultaneously detecting 17 pathogens for non-disease diagnostic purposes. The beneficial effects of this invention are that it designs 17 primer sets to simultaneously amplify DNA fragments containing specific target sequences of 17 pathogens in multiplex PCR, and performs single-base extension of these products using highly efficient single-base primers. Simultaneous detection and identification of 17 pathogens can be achieved using MALDI-TOF MS mass spectrometry, with accurate and highly specific detection results.

The invention relates to a super-multiplex digital PCR (polymerase chain reaction) detection method for simultaneously detecting multiple pathogens of shrimps and application of the super-multiplex digital PCR detection method. According to the digital PCR primer, the probe, the kit and the detection method for shrimp pathogen detection, pathogens aiming at 18 shrimp pathogens can be rapidly, accurately and ultrasensitively detected. By detecting pathogens in the prawns, the health condition of the prawns in intensive prawn culture can be evaluated in time, disease transmission can be prevented, intervention measures can be taken in time, the risk of disease transmission to prawn groups in intensive culture can be reduced, serious economic loss can be avoided, and the application value is extremely high. Accurate identification of specific pathogens in a shrimp sample is very important for designing a proper control strategy, because each pathogen may require different prevention and management methods, drug use conditions can be effectively reduced. The shrimp pathogen detection can improve the culture benefit, and is also beneficial for developing the epidemic disease prevention and control work of aquatic animals and the inspection and quarantine monitoring plan of main aquaculture variety offspring seeds.