156 results about "Pathogen detection" patented technology

The invention provides a construction method of a bacterial pathogen detection model based on nanopore adaptive sampling and deep learning, and a method, equipment, a medium and a program product for detecting bacterial pathogens by applying the model, and relates to the technical field of nanopore sequencing. The bacterial pathogen detection method comprises the following steps: obtaining a water sample to be detected; and inputting a to-be-detected water sample into the detection model constructed in the invention to obtain a detection result of the to-be-detected water sample. The sensitivity and the accuracy of pathogen detection are obviously improved, and the improvement on prevention and control of water-borne diseases is facilitated.

This invention discloses primer compositions, detection products, and applications for detecting 17 pathogens, relating to the field of biotechnology. A primer composition for detecting 17 pathogens includes 17 pairs of amplification primers and 17 extension probes, as shown in SEQ ID NO: 1-51, respectively. This invention also provides detection products comprising the above primer compositions and their application in simultaneously detecting 17 pathogens for non-disease diagnostic purposes. The beneficial effects of this invention are that it designs 17 primer sets to simultaneously amplify DNA fragments containing specific target sequences of 17 pathogens in multiplex PCR, and performs single-base extension of these products using highly efficient single-base primers. Simultaneous detection and identification of 17 pathogens can be achieved using MALDI-TOF MS mass spectrometry, with accurate and highly specific detection results.

The invention relates to the field of molecular diagnosis and nucleic acid amplification, in particular to a recombinase polymerization amplification method based on Twinkle helicase enhancement and application of the recombinase polymerization amplification method. According to the invention, Twinkle helicase is introduced into an RPA system for the first time, and primer combination and template unlinking are assisted by utilizing ATP-dependent unlinking activity and DNA annealing activity of the Twinkle helicase, so that the amplification efficiency and the detection sensitivity are remarkably improved. The method is suitable for plasmid DNA, a high GC template and a clinical nucleic acid sample, and can be used for constructing an RPA kit containing Twinkle, so that the application of the RPA kit in pathogen detection, molecular diagnosis and on-site rapid detection is expanded. According to the invention, the defects of the existing RPA technology in sensitivity and stability are overcome, and a new solution is provided for developing a novel nucleic acid self-detection and field detection tool.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention relates to the technical field of image or video recognition or understanding, and discloses an areca yellows early warning method based on regular economic forest multi-dimensional symptom analysis, in the method, the unique morphology of areca and various morphological changes which are invisible under trees but visible in remote sensing during the period that areca suffers from yellows are fully utilized, and the areca yellows early warning effect is achieved. And the multiple symptoms are combined to form a multi-symptom matching degree, so that the misrecognition problem can be eliminated in an auxiliary manner when specific symptoms exist. When the specific symptoms do not exist, whether the disease of the current areca-nut forest belongs to the infectious disease or not is indirectly judged according to the distribution characteristics of the multi-symptom matching degree in time and space by utilizing the distribution characteristics and the disease course development rule when the areca-nut is cultivated as an economic arbor, and if yes, the disease of the current areca-nut forest belongs to the infectious disease; and considering that the areca yellows occupy a large proportion in the areca infectious diseases, the pathogen detection cannot be infeasible due to entrainment of a large number of irrelevant diseased plant samples when the pathogen detection is carried out at the moment. The above points are combined to realize early warning of areca yellows.

The invention belongs to the technical field of biology, and relates to a pathogen detection technology based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, in particular to a tubercle bacillus detection and activity determination method based on fluorescent T7-CRISPR. The first purpose is to provide a reagent combination for detecting viable tubercle bacillus, and the reagent combination comprises a) a T7 reverse transcription reaction reagent and b) a CRISPR-Cas13a reaction system; a) the T7 reverse transcription reaction reagent comprises a T7 reverse transcription primer and a T7 reverse transcriptase; and b) the CRISPR-Cas13a reaction system comprises specific crRNA, a fluorescence labeled ssRNA reporter, a reaction buffer solution, a Cas13a protein, RNase-Free H2O and a T7 reverse transcriptase. The invention also provides application of the reagent combination in detection of viable tubercle bacillus. The primer combination disclosed by the invention has the advantages of strong specificity and high sensitivity in detection application, can realize rapid detection and visual detection, and is low in detection cost, simple and convenient to operate and suitable for large-scale clinical application.

The invention discloses a primer probe group and a kit for joint detection of mycoplasma genitalium, ureaplasma urealyticum and chlamydia trachomatis, and belongs to the technical field of molecular biological detection. The primer probe group comprises a primer probe group A for directly detecting a mycoplasma genitalium mgpB gene, a primer probe group B for directly detecting a ureaplasma urealyticum ureB gene and a primer probe group C for directly detecting a chlamydia trachomatis ompA gene; the kit comprises a PCR (Polymerase Chain Reaction) reaction solution and the primer probe group. Nucleic acid extraction is not needed, the detection cost is greatly reduced, the experiment time is shortened, meanwhile, the specificity and accuracy are still guaranteed, meanwhile, the sensitivity is improved, and the method has wide application prospects in the fields of non-gonococcal genital tract infection main pathogen detection, infertility and prenatal and postnatal care screening and the like. And the kit has important significance on clinical diagnosis, early treatment and prevention of diseases, prevalence and the like.

The invention provides a tsutsugamushi disease pathogen detection primer, a probe and a kit, the sequences of the primer and the probe in an asymmetric primer ERA detection system are designed and screened according to the target sequence of a tsutsugamushi disease pathogen gene, and the proportion of upstream and downstream primers and an amplification reaction system are optimized; the nucleic acid sequence of the tsutsugamushi disease pathogen can be specifically recognized, and non-specific binding with other irrelevant nucleic acid is reduced, so that the detection specificity is improved; meanwhile, efficient amplification of the tsutsugamushi disease pathogen nucleic acid is achieved, accurate detection can be achieved even under the condition that the virus nucleic acid content is extremely low, and therefore the detection sensitivity and the amplification efficiency are improved.

The invention provides an ichthyophthirius multifilis detection reagent and detection method based on an enzymatic isothermal amplification technology, and belongs to the technical field of pathogen detection. The invention provides a primer probe group for detecting ichthyophthirius multifilis by using an enzymatic isothermal amplification (ERA) method, and a CO1 gene of the ichthyophthirius multifilis is used as a target sequence. Based on the ERA primer probe group designed by the invention, a test strip type isothermal detection kit and a fluorescent isothermal detection kit are developed, and are respectively associated with'test strip type visual detection 'and'fluorescent quantitative detection'. The two ERA detection kits developed by the invention have the advantages of strong specificity, high detection sensitivity, high detection efficiency and no need of high-temperature equipment, and can meet the requirements of on-site rapid visual detection. According to the invention, the technical blank of on-site rapid diagnosis of ichthyophthirius multifilis is filled, and a key technical support is provided for early prevention and control of aquaculture diseases.

The invention discloses a detection reagent, a kit and a detection method for pathogens of micropterus salmoides. The micropterus salmoides pathogen detection reagent comprises primers and probes for specifically amplifying an infectious spleen and kidney necrosis virus, a viral nervous necrosis virus, flavobacterium columnar, a perch rhabdovirus, a micropterus salmoides double-RNA virus, edwardsiella tarda, nocardia and a micropterus salmoides iridovirus; specific upstream and downstream primers and probes of the eight pathogens are sequences as shown in SEQ ID NO.1 to SEQ ID NO.24 in sequence. The micropterus salmoides pathogen detection reagent and method can detect eight pathogens at the same time, have the advantages of high sensitivity, high specificity, good repeatability and the like, provide reliable basis for prevention and control of the pathogens, also improve the prevention and control work efficiency, and have good application prospects. The kit is especially suitable for large-scale rapid screening and mixed infection diagnosis of aquaculture pathogens, and has important value and significance for prevention and treatment of micropterus salmoides pathogens.

This invention discloses a functionalized biomodified electrode for rapid pathogen detection and its preparation method, relating to the fields of biosensing and electrochemical detection technology. It includes a glassy carbon electrode substrate, the surface of which is sequentially modified with a reduced graphene oxide layer, an electropolymerized polydopamine film, and an electrodeposited gold nanoparticle layer, forming a composite nanomaterial modified electrode. Antibodies are captured and fixed to the electrode surface through covalent interactions. This functionalized biomodified electrode for rapid pathogen detection and its preparation method, using the same electrochemical workstation, first electropolymerizes dopamine to form a film and then electrodeposits gold nanoparticles. This allows for precise control of film thickness, gold particle size, and coverage density by parameters such as polymerization potential, scan cycles, deposition potential, and deposition time. This avoids batch-to-batch fluctuations caused by dissolved oxygen and solution disturbances in traditional chemical methods, reducing the relative standard deviation of peak current detection from different batches of electrodes from 18.7% to 4.2%–4.5%.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The application discloses a primer probe composition which is composed of a forward primer, a reverse primer and a probe primer. The sequence of the forward primer is shown as SEQ ID No. 1, the sequence of the reverse primer is shown as SEQ ID No. 2, and the sequence of the probe is shown as SEQ ID No. 3. The application establishes a detection method for Pseudomonas syringae pv. actidii, which comprises the following steps: extracting genomic DNA of a sample to be detected, taking the genomic DNA as a template, and performing RAA detection by using the primer composition; and determining whether the sample to be detected contains Pseudomonas syringae pv. actidii according to a strip of a LFD test paper. The detection method provided by the application has a minimum detection sensitivity of 1 pg / µL for Pseudomonas syringae pv. actidii DNA, and has a minimum detection limit of 1×10 3 cfu / mL for a bacterial suspension, and can provide a new technical means for early diagnosis and disease prevention and control of bacterial canker disease of kiwifruit.

The application discloses a livestock breeding poultry quarantine sampling device, and particularly relates to the technical field of poultry quarantine sampling, and comprises a positioning disc, a positioning sampling assembly is arranged on the positioning disc, the positioning sampling assembly comprises a swivel ring arranged on one side of the positioning disc, a screw rod capable of being displaced up and down is slidly connected to the middle part of the swivel ring, and a top disc is movably connected to the top end of the screw rod. The application effectively removes interfering substances in the sample, avoids the influence of impurities on subsequent pathogen detection results, improves the reliability of quarantine data, and improves the accuracy and comprehensiveness of quarantine results. The blood sample is used for detecting pathogen antibodies, immune state and other indexes, and the fecal sample is used for checking intestinal pathogens, parasite eggs and the like. The two types of samples are complementary and verified, the accuracy and comprehensiveness of the quarantine results are greatly improved, and the problem that a single sample cannot comprehensively judge the health status of poultry is effectively solved.

The present application relates to the technical field of animal health monitoring, and relates to a wild animal non-invasive genome monitoring and population health evaluation system. A time indication marker is generated based on the ratio of a predetermined indicator bacteria genus, and microenvironment data is input into a pre-trained microbial succession model to intelligently infer the actual exposure time of the sample in the wild. Then, the initial intestinal flora map of the sample at the instant of discharge is reconstructed. The high-fidelity flora map after reconstruction is further deeply coupled with health evaluation to realize scientific diagnosis and early warning of the physiological state of wild animal individuals. The accuracy of health evaluation, nutritional status analysis and pathogen detection is greatly improved, and major misjudgments such as misjudging environmental bacteria as pathogenic bacteria or misdiagnosing flora natural decay as ecological imbalance are avoided. A stable and reliable technical tool is provided for wild animal protection management.

According to the oligonucleotide functionalized gold nanoparticle-based multiple lateral chromatography biological detection system and method provided by the invention, high-sensitivity and programmable nucleic acid target detection is realized through functionalized combination of gold nanoparticles and multiple oligonucleotide probes. The system of the invention comprises: 1) a tunable oligonucleotide-AuNP conjugate (each AuNP is linked to 2-3 thiolated probes); 2) a standardized test strip (containing a streptavidin detection line and an ACTB-828 quality inspection line); and 3) a sandwich hybridization mechanism (the target nucleic acid is combined with the AuNP probe and the biotinylation report oligonucleotide at the same time). The method has the beneficial effects that (1) due to the target-induced plasma aggregation effect, an optical signal is enhanced through AuNP close-range aggregation; and (2) a general test paper structure can adapt to a new target only by adjusting a probe sequence. Experiments show that the detection limit reaches 50 pM, and the room temperature stability is gt; the method is suitable for the fields of rapid pathogen detection, on-site diagnosis and the like.

This application provides specific primers, rapid detection methods, and applications for detecting root rot of *Imperata cylindrica*. The primer pairs for detecting or assisting in the detection of root rot of *Imperata cylindrica* include primer Rd-F and primer Rd-R. The nucleotide sequence of primer Rd-F is shown in SEQ ID No. 1, and the nucleotide sequence of primer Rd-R is shown in SEQ ID No. 2. This application designs specific primer pairs Rd-F / Rd-R based on the ITS sequence of the root-invasive fungus isolated from the roots of *Imperata cylindrica* in Qinghai Province, and establishes a corresponding PCR detection method for specific identification. Rhypophila deceiving The results show that this method is rapid, specific, sensitive, and easy to operate. It can be used for pathogen detection, early diagnosis, and disease control of *Imperata cylindrica* root rot, providing technical support for precise monitoring and sustainable management of grassland diseases.

The application discloses a nano antibody against Ebola virus VP40 protein and application thereof in Ebola virus detection, wherein the nano antibody against Ebola virus VP40 protein is a 3E07 antibody. The nano antibody against Ebola virus VP40 protein prepared by the application has the characteristics of high sensitivity and wide binding spectrum. A double antibody sandwich enzyme-linked immunosorbent assay method constructed by 3E07 and 3F06 antibodies can efficiently detect VP40 proteins and virus-like particles of various Ebola virus subtypes such as Zaire, Sudan and Beni, wherein the detection sensitivity of the Ebola virus VP40 protein of Zaire type can reach 0.039 ng / mL, and the antibody combination has a wide application prospect in the diagnosis and pathogen detection of Ebola virus disease.

A pathogen detection system and method are disclosed. The pathogen detection system includes a pathogen sensor comprising a plasmonically active pathogen sensing area that comprises a hybrid structure of electrically conductive surface and metal nanofractals, forming a surface enhanced Raman spectroscopic (SERS) substrate and a controller comprising a memory storing one or more trained machine learning algorithms, that perform label-free identification and differentiation of a pathogen or the pathogen and a pathogen mutation based on acquired Raman spectra.

This invention provides a primer-probe combination, reagents, methods, and applications for detecting Brucella vaccine strain RB51, belonging to the field of pathogen detection technology. The primer-probe combination for detecting Brucella vaccine strain RB51 of this invention includes a forward primer RB51-F, a reverse primer RB51-R, and a probe RB51-P; the forward primer RB51-F is shown in SEQ ID NO:1, the reverse primer RB51-R is shown in SEQ ID NO:2, and the probe RB51-P is shown in SEQ ID NO:3. The fluorescent PCR and digital PCR detection methods established based on the primer-probe combination of this invention have high sensitivity, good specificity, fast detection speed, and high throughput, enabling rapid detection of Brucella vaccine strain RB51, making differential diagnosis more accurate, economical, efficient, and convenient.

The invention provides a library building method for pathogen detection, a kit and application thereof. The method comprises the following steps: S1) obtaining a first product of a nucleic acid single chain of a sample, wherein the first product is combined with a random primer; s2) performing complementary chain synthesis on the first product to obtain a second product; s3) purifying the second product to obtain a third product; s4) carrying out fragmentation treatment on the third product, connecting a joint, and directly carrying out library amplification PCR reaction to obtain a nucleic acid library of the sample; in the step (S4), a product obtained after joint connection is not purified; according to the volume fraction, the fraction ratio of a product obtained after the joint is connected in a library amplification PCR reaction system to the PCR amplification primer to the PCR amplification reagent is 4: (0.5-2): (4-5). The method can solve the problems of complex steps, long time consumption and small sample input volume of a co-construction library method in the existing NGS detection technology, and is suitable for the field of pathogenic microorganism library construction.

To simply perform the totalization and presentation of the inspection results of a plurality of pathogens.SOLUTION: A pathogen test result aggregation system comprising a display control unit that displays an input screen for inputting a test result indicating whether each pathogen is positive or negative for a plurality of pathogens in order to easily aggregate and present the test results for the plurality of pathogens, an aggregation unit that aggregates the test results, and a screen generation unit that generates an aggregation screen regarding the test result of each of the plurality of pathogens.SELECTED DRAWING: Figure 1

The invention discloses LAMP (loop-mediated isothermal amplification) method detection primers and a detection system for peach bacterial perforation bacterium pantoea ananatis and application, and belongs to the technical field of plant germ detection. The method comprises the following steps: firstly, screening a conserved DNA sequence of peach bacterial perforation bacteria pantoea ananatis, designing an LAMP primer group, and verifying the specificity, the detection method and the sensitivity of the LAMP primer group. A detection system comprises 1.4 mM of dNTPs, 4 mM of MgSO4, 0.1 mu M of F3 / B3 and 1.6 mu M of FIP / BIP, and the result can be judged by incubating the mixed system in a water bath at the constant temperature of 64 DEG C for 60 minutes. The system can detect a sample containing 10 pg / mu L of Pantoea ananatis genome at least. The LAMP detection method based on Pantoea ananas specificity provided by the invention has the advantages of simplicity and convenience in operation, accuracy, sensitivity, strong specificity, high visualization degree and the like, is suitable for rapid field diagnosis of Pantoea ananas, and has remarkable field application value.

The present application relates to the technical field of pathogen detection, and particularly relates to a respiratory system infection pathogen rapid detection method based on multiplex PCR. After a baseline corrected fluorescence intensity sequence is obtained, valid fluorescence intensity sequences are screened according to whether the signal amplification trend of the baseline corrected fluorescence intensity sequence conforms to the characteristics of amplification kinetics morphology. Then, the kinetic morphology characteristics corresponding to each valid fluorescence intensity sequence are quantified through multi-dimensional morphology analysis on the basis of the frequency domain and the time domain. Furthermore, each valid fluorescence intensity sequence is quantified for a morphology determination value based on the relative deviation of the amplification morphology characteristic vector between the prior negative control hole and the prior positive control hole. Thus, the signal property is more accurately defined based on the morphology determination value, and the accuracy of the respiratory system infection pathogen detection is higher.

The invention discloses a food-borne pathogen detection device and method based on a micro-fluidic chip, the food-borne pathogen detection device comprises a shell, the interior of the shell is fixedly connected with an isolation plate, the surface of the isolation plate is provided with a setting cavity, the setting cavity is internally provided with a quick disassembly mechanism for quickly disassembling a detection module, and a fixing mechanism comprises a spring arranged in the setting cavity. According to the quick release mechanism driven by the pressing type inert gas, an operator can complete disassembly and replacement of core components such as the detection module and the power supply module only by pressing and plugging without any professional tool, and the whole process can be completed in a short time; the design effectively solves the problem that a whole machine needs to be returned to a factory for maintenance after a single module of an existing integrated device breaks down, and in field detection scenes such as farmer's markets and cold chain ports, even if the problems that a fluid driving module is blocked, a temperature control module breaks down and the like occur, detection work can be rapidly recovered by replacing a standby module, and interruption of the detection process is avoided.

To simply sum up test results on a plurality of pathogens.SOLUTION: A pathogen test result aggregation system comprising an information processing terminal comprising a display control unit that displays an input screen for inputting a test result indicating whether each pathogen is positive or negative for a plurality of pathogens, and a transmission unit that transmits the test results for the plurality of pathogens input from the input screen, and a server that aggregates the test results transmitted from the transmission unit and generates an aggregation screen indicating the number of positives for each of the plurality of pathogens.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of pathogen detection, and particularly relates to a freeze-drying PCR reagent, a kit and a method for detecting nucleic acids of six respiratory pathogens. Through optimized and screened freeze-drying stabilizer combination, key components such as primers and probes corresponding to each target and an internal standard, a buffer system and the like are successfully prepared into freeze-dried powder with stable performance under the condition of 2-8 DEG C through a freeze-drying process, and meanwhile, the freeze-dried powder has high sensitivity and high specificity in detection of pathogens, and has a good application prospect. The condition requirements and the cost of the fluorescent PCR reagent in the logistics transportation and storage process are obviously reduced.

An aspirating detection system for monitoring for the presence of a pathogen, the aspirating detection system including: a network of one or more pipes for sampling air from a plurality of locations monitored by the aspirating detection system; a sensor unit 3 comprising a housing 13 fluidly connected to the network of one or more pipes, and a biosensor 12 mounted within the housing, the biosensor being configured to monitor for the presence of the pathogen; and an aspirator 15 configured to draw airflow through the network of one or more pipes and through the biosensor 12.

The invention relates to a physical host removal method for a respiratory tract sample, and belongs to the technical field of pathogen detection. The invention aims to combine a physical method to remove hosts and a chemical method to remove hosts, establish an efficient and broad-spectrum respiratory tract sample host removing method by optimizing physical homogenization parameters and combining the chemical method, and break through the bottleneck in the prior art. The detection method disclosed by the invention has the advantages that 1) the detection method can be suitable for sputum samples without adding treatment steps; 2) the human source removal efficiency is high, and compared with a chemical method for removing hosts widely applied at present, the host removal efficiency is high, and the number of microbial sequences is high; and 3) the operation is simple and convenient, and hosts can be removed within a short time.

The invention provides a primer probe combination and a portable kit for African swine fever pathogen detection, and relates to the technical field of biological detection. The primer probe combination comprises an upstream primer, a downstream primer and a probe, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.7; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.8; and the nucleotide sequence of the probe is as shown in SEQ ID NO. 9. The primer probe combination adopts a specific sequence obtained by screening SEQ ID NO.7-9, the detection sensitivity reaches 1 copies / T, and non-specific amplification is avoided. Through double labeling of biotin and a fluorophore and modification of tetrahydrofuran and a blocking group, the group is suitable for isothermal amplification and lateral chromatography technologies, visual color development of a specific amplification product can be realized without a variable-temperature instrument, and the group is suitable for low-cost field rapid detection.