254 results about "Pathogen detection" patented technology

The invention discloses a hydrogel chip as well as a preparation method and application thereof. The hydrogel chip comprises a chip shell, a hydrogel array and a sample flow channel, wherein the hydrogel array and the sample flow channel are arranged in the chip shell. The hydrogel chip provided by the invention is simple in manufacturing process, convenient to use and low in preparation cost, can realize ultra-multiple pathogen detection, can perform PCR, melting curve analysis and real-time quantitative PCR, can flexibly set detection items according to actual requirements, is high in expandability, can realize multi-target rapid detection, is simple to operate, and is suitable for large-scale popularization and application. And the hydrogel chip is a totally closed reaction system, so that pollution can be effectively prevented.

The invention belongs to the technical field of biological detection, and particularly discloses a targeted sequencing primer group and a kit for detecting common pathogens of children and drug-resistant genes thereof. The primer group comprises 145 primer pairs of common children pathogen primer groups and 67 primer pairs of drug-resistant gene primer groups of the common children pathogen primer groups. According to the kit provided by the invention, nucleic acid in a clinical sample is directly extracted, 212 pairs of specific primers are adopted to realize multi-targeted amplification of a pathogen target area, a high-throughput sequencing platform is adopted to accurately identify pathogens and drug-resistant mutant genes thereof, 72 common children pathogens and drug-resistant genes thereof can be detected at the same time, and the kit has a wide application prospect. More accurate diagnosis information is provided for clinic, a key technical support is provided for early-stage accurate treatment of children infectious diseases, and the technical problems that in the prior art, in children pathogen detection, the detection speed is low, the number of missed detection is large, and the drug-resistant blind area is large are solved.

The invention discloses a pathogen detection primer group, a kit and application in eye infection pathogen detection, the detection primer group comprises 162 pairs of primers for 54 pathogenic microorganisms, the interference competition among the primers is small, the 54 pathogenic microorganisms can be detected at the same time by randomly selecting one pair of primers for each pathogenic microorganism, and the detection efficiency is high. When all 162 pairs of primers are selected, the sensitivity and specificity of species identification can be improved, the specific and high-sensitivity detection of bacteria, fungi, viruses, parasites and accurately identified species can be met, and the requirements of clinical accurate diagnosis are met.

The invention provides a construction method of a bacterial pathogen detection model based on nanopore adaptive sampling and deep learning, and a method, equipment, a medium and a program product for detecting bacterial pathogens by applying the model, and relates to the technical field of nanopore sequencing. The bacterial pathogen detection method comprises the following steps: obtaining a water sample to be detected; and inputting a to-be-detected water sample into the detection model constructed in the invention to obtain a detection result of the to-be-detected water sample. The sensitivity and the accuracy of pathogen detection are obviously improved, and the improvement on prevention and control of water-borne diseases is facilitated.

This invention discloses primer compositions, detection products, and applications for detecting 17 pathogens, relating to the field of biotechnology. A primer composition for detecting 17 pathogens includes 17 pairs of amplification primers and 17 extension probes, as shown in SEQ ID NO: 1-51, respectively. This invention also provides detection products comprising the above primer compositions and their application in simultaneously detecting 17 pathogens for non-disease diagnostic purposes. The beneficial effects of this invention are that it designs 17 primer sets to simultaneously amplify DNA fragments containing specific target sequences of 17 pathogens in multiplex PCR, and performs single-base extension of these products using highly efficient single-base primers. Simultaneous detection and identification of 17 pathogens can be achieved using MALDI-TOF MS mass spectrometry, with accurate and highly specific detection results.

The invention relates to an AI-based M-ROSE medical image pathogen detection method and device, and the method comprises the following steps: combining fluorescence microscopic imaging, an AI model and a computer vision technology to realize automatic analysis of fungus detection and mycobacterium tuberculosis detection, and comprises the following steps: constructing a medical image fungus fluorescence image classification method based on deep learning; carrying out fluorescence staining on a fungus sample, carrying out automatic scanning identification, and carrying out automatic identification and classification through an AI model; a medical image tubercle bacillus detection method based on deep learning is constructed, a tubercle bacillus sample is subjected to acid-fast staining or auramine O staining and then enters an automatic scanning recognition system, and whether tubercle bacillus exists or not is automatically judged in combination with a target detection model; and obtaining an automatic identification result, and judging whether fungus types exist in the sample or not or whether tubercle bacillus exists in the sample. The detection sensitivity, the automation degree and the clinical applicability can be improved.

The invention provides an MNP marker site, a primer group and a kit for identifying multiple pathogens in sewage and application of the MNP marker site, the primer group and the kit. The MNP marker site comprises at least one of MNP-1 to MNP-19, the primer group comprises at least one of a first primer pair to a nineteenth primer pair, and a forward primer of the first primer pair, a reverse primer of the first primer pair to a forward primer of the nineteenth primer pair and a reverse primer of the nineteenth primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 38 in a sequence table. The invention provides MNP marker sites for identifying multiple pathogens in sewage, a primer group, a kit and application, the primer group aims at 19 MNP marker sites, 1-3 marker sites are detected for each pathogen, the pathogens in the multiple types of sewage can be identified, the comprehensive, efficient and accurate detection effect is achieved, and the primer group is suitable for identifying the multiple pathogens in the sewage.

The invention discloses a pathogenic bacteria detection kit and a detection method based on RPA-CRISPR / Cas12a. According to the detection method, multiple pathogenic bacteria are synchronously amplified by adopting an RPA technology, and then amplification products are detected by adopting a CRISPR / Cas12a detection system, so that synchronous and rapid detection of the multiple pathogenic bacteria is realized; the pathogenic bacteria detection kit based on RPA-CRISPR / Cas12a uses an RPA technology to perform isothermal amplification on a target gene, does not need to depend on a large-scale thermal cycle instrument, has low experimental requirements, can realize synchronous amplification of multiple pathogenic bacteria molecules within 10-20 min, and can realize synchronous real-time detection of multiple pathogenic bacteria by combining a CRISPR / Cas12a system, so that the detection kit has the advantages of high sensitivity, high specificity, high sensitivity, high sensitivity and the like. The whole pathogen detection process consumes less time and does not exceed 1h; the detection sensitivity is also relatively high and reaches 100 CFU / mL; and synchronous and rapid detection of various pathogenic bacteria in hospital infection is realized.

The invention relates to the field of molecular diagnosis and nucleic acid amplification, in particular to a recombinase polymerization amplification method based on Twinkle helicase enhancement and application of the recombinase polymerization amplification method. According to the invention, Twinkle helicase is introduced into an RPA system for the first time, and primer combination and template unlinking are assisted by utilizing ATP-dependent unlinking activity and DNA annealing activity of the Twinkle helicase, so that the amplification efficiency and the detection sensitivity are remarkably improved. The method is suitable for plasmid DNA, a high GC template and a clinical nucleic acid sample, and can be used for constructing an RPA kit containing Twinkle, so that the application of the RPA kit in pathogen detection, molecular diagnosis and on-site rapid detection is expanded. According to the invention, the defects of the existing RPA technology in sensitivity and stability are overcome, and a new solution is provided for developing a novel nucleic acid self-detection and field detection tool.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention relates to the technical field of image fusion, and discloses a lightweight and efficient multi-modal data fusion method. According to the method, an improved MobileNetV3-Small network is adopted as a basic feature extractor, parallel branch design in a training stage is optimized through a structure re-parameterization technology, and the parallel branch design is combined into single convolution in a reasoning stage, so that the calculation cost is remarkably reduced; resNet50 is introduced as a teacher model, a soft label is generated through temperature scaling, a student model is guided in combination with KL divergence loss and cosine feature alignment loss, and high-precision feature extraction of a lightweight network is realized; a dynamic weight fusion mechanism is designed, microscopic fluorescence and microfluidic fluorescence image features are fused, and multi-modal information contribution is balanced; aiming at the characteristics of different modal images, preprocessing methods such as guided filtering, CLAHE contrast enhancement, time sequence difference artifact removal and the like are respectively adopted, and effective signals are enhanced. According to the algorithm, the pathogen detection precision is ensured, the model parameter quantity is reduced, and an efficient solution capable of being deployed in an embedded mode is provided for livestock and poultry epidemic disease screening.

The invention discloses a primer probe combination and a kit for detecting nucleic acids of 14 respiratory pathogens and application, and belongs to the technical field of pathogen detection. The technical problem to be solved is that the detection of respiratory pathogens at the present stage is not only limited by a complex operation process, but also is difficult to meet the clinical rapid diagnosis requirement. Meanwhile, due to the inherent technical bottleneck, the problems of insufficient sensitivity, high false negative rate and the like exist. According to the technical scheme, the primer probe combination for simultaneously detecting 14 respiratory pathogens is provided, and based on the Real-time PCR technology, rapid, specific and accurate detection of the 14 respiratory pathogens is achieved.

The invention relates to the technical field of image or video recognition or understanding, and discloses an areca yellows early warning method based on regular economic forest multi-dimensional symptom analysis, in the method, the unique morphology of areca and various morphological changes which are invisible under trees but visible in remote sensing during the period that areca suffers from yellows are fully utilized, and the areca yellows early warning effect is achieved. And the multiple symptoms are combined to form a multi-symptom matching degree, so that the misrecognition problem can be eliminated in an auxiliary manner when specific symptoms exist. When the specific symptoms do not exist, whether the disease of the current areca-nut forest belongs to the infectious disease or not is indirectly judged according to the distribution characteristics of the multi-symptom matching degree in time and space by utilizing the distribution characteristics and the disease course development rule when the areca-nut is cultivated as an economic arbor, and if yes, the disease of the current areca-nut forest belongs to the infectious disease; and considering that the areca yellows occupy a large proportion in the areca infectious diseases, the pathogen detection cannot be infeasible due to entrainment of a large number of irrelevant diseased plant samples when the pathogen detection is carried out at the moment. The above points are combined to realize early warning of areca yellows.

The invention belongs to the technical field of biology, and relates to a pathogen detection technology based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, in particular to a tubercle bacillus detection and activity determination method based on fluorescent T7-CRISPR. The first purpose is to provide a reagent combination for detecting viable tubercle bacillus, and the reagent combination comprises a) a T7 reverse transcription reaction reagent and b) a CRISPR-Cas13a reaction system; a) the T7 reverse transcription reaction reagent comprises a T7 reverse transcription primer and a T7 reverse transcriptase; and b) the CRISPR-Cas13a reaction system comprises specific crRNA, a fluorescence labeled ssRNA reporter, a reaction buffer solution, a Cas13a protein, RNase-Free H2O and a T7 reverse transcriptase. The invention also provides application of the reagent combination in detection of viable tubercle bacillus. The primer combination disclosed by the invention has the advantages of strong specificity and high sensitivity in detection application, can realize rapid detection and visual detection, and is low in detection cost, simple and convenient to operate and suitable for large-scale clinical application.

The invention discloses a primer probe group and a kit for joint detection of mycoplasma genitalium, ureaplasma urealyticum and chlamydia trachomatis, and belongs to the technical field of molecular biological detection. The primer probe group comprises a primer probe group A for directly detecting a mycoplasma genitalium mgpB gene, a primer probe group B for directly detecting a ureaplasma urealyticum ureB gene and a primer probe group C for directly detecting a chlamydia trachomatis ompA gene; the kit comprises a PCR (Polymerase Chain Reaction) reaction solution and the primer probe group. Nucleic acid extraction is not needed, the detection cost is greatly reduced, the experiment time is shortened, meanwhile, the specificity and accuracy are still guaranteed, meanwhile, the sensitivity is improved, and the method has wide application prospects in the fields of non-gonococcal genital tract infection main pathogen detection, infertility and prenatal and postnatal care screening and the like. And the kit has important significance on clinical diagnosis, early treatment and prevention of diseases, prevalence and the like.

The invention provides a tsutsugamushi disease pathogen detection primer, a probe and a kit, the sequences of the primer and the probe in an asymmetric primer ERA detection system are designed and screened according to the target sequence of a tsutsugamushi disease pathogen gene, and the proportion of upstream and downstream primers and an amplification reaction system are optimized; the nucleic acid sequence of the tsutsugamushi disease pathogen can be specifically recognized, and non-specific binding with other irrelevant nucleic acid is reduced, so that the detection specificity is improved; meanwhile, efficient amplification of the tsutsugamushi disease pathogen nucleic acid is achieved, accurate detection can be achieved even under the condition that the virus nucleic acid content is extremely low, and therefore the detection sensitivity and the amplification efficiency are improved.

The invention discloses a primer composition for detecting drug resistance of mycobacterium tuberculosis, a related product and application, and belongs to the field of pathogen detection. The primer composition comprises a primer pair aiming at an rpoB gene, a primer pair aiming at a katG gene, a primer pair aiming at an inhA gene, a primer pair aiming at an ahpC gene, a primer pair aiming at a gyrA gene and a primer pair aiming at a gyrB gene. According to the invention, mutation of mycobacterium tuberculosis complex and rifampicin, isoniazide and quinolone drug resistance related genes rpoB, katG, inhA, ahpC, gyrA and gyrB genes in sputum of a tuberculosis patient can be detected at one time, single-tube multiple high-sensitivity detection is realized, wild genes and mutant genes are accurately distinguished, and serious cross reaction is avoided.

A galley monument for a cabin area of an aircraft including a first monument stack, a second monument stack adjacent to the first monument stack, a tray within the second monument stack to secure a rapid pathogen tester, a slidably moveable support located below the tray to secure a plurality of tools to use along with the rapid pathogen tester, and at least one light within the tray to irradiate the tools placed within the support.

The invention discloses an anti-Ebola virus VP40 protein nano antibody and application of the anti-Ebola virus VP40 protein nano antibody in Ebola virus detection. The anti-Ebola virus VP40 protein nano antibody is a 3E07 antibody. The anti-Ebola virus VP40 protein nano antibody prepared by the invention has the characteristics of high sensitivity, wide binding spectrum and the like. A double-antibody sandwich enzyme-linked immunosorbent assay method constructed by the 3E07 and 3F06 antibodies can be used for efficiently detecting VP40 proteins and virus-like particles of various subtype Ebola viruses such as Zaire, Sudan, Bandong and the like, and the detection sensitivity for the VP40 proteins of the Zaire type Ebola viruses can reach 0.039 ng / mL; the antibody combination has a wide application prospect in diagnosis and pathogen detection of Ebola virus diseases.

The present invention discloses a detection primer, a kit and its application for Vibrio parahaemolyticus, comprising an upstream primer F and a downstream primer R; the upstream primer R is selected from one or more of SEQ ID NO.10, SEQ ID NO.26 and SEQ ID NO.40, and the downstream primer R is selected from one or more of SEQ ID NO.11, SEQ ID NO.27 and SEQ ID NO.41. The primer set can be used in the detection of Vibrio parahaemolyticus and the preparation of Vibrio parahaemolyticus detection preparations and kits for non-diagnostic purposes. The present application establishes a standardized VpTPD pathogen detection method and develops a rapid, convenient, highly sensitive, highly specific, economical and practical detection primer and kit that can be widely promoted for pathogen detection, thereby solving the effective prevention and efficient monitoring of Vibrio parahaemolyticus.

The application discloses nanobodies against E protein of chikungunya virus and application in virus detection, and the nanobodies against E protein of chikungunya virus include N055 and 10G4 antibodies, and both have high affinity and binding activity with antigens. A double antibody sandwich enzyme-linked immunosorbent assay method constructed by the nanobodies of the N055 and 10G4 antibodies can efficiently detect E protein of chikungunya virus and virus-like particles, and the detection sensitivity can reach 49 pg / mL, and the antibody combination has wide application prospects in diagnosis and pathogen detection of chikungunya virus disease.

The present invention discloses label oligonucleotides, reagents or kits for quality control, and their preparation methods and applications. By randomly generating oligonucleotide sequences and performing multiple screenings under specific screening conditions, the optimal oligonucleotide label sequence is finally obtained. Based on such sequences, specific primers and corresponding probes are further designed to construct a simple, rapid, and highly specific and sensitive detection system. The obtained label oligonucleotide sequence can be introduced as a unified label into different pathogen nucleic acid standard substances or quality control products, effectively solving the different detection efficiencies of different detection reagents for different pathogens, standardizing a series of products and improving the accuracy of quantitative results, and helping to optimize the pathogen detection system; at the same time, the label sequence is introduced into positive nucleic acid quality control products, which can also effectively solve the effective identification of false positive results of the test samples caused by positive quality control contamination.

The application discloses a swab collecting and transporting device for respiratory pathogen detection and relates to the technical field of medical devices.The device comprises a tube body and a tube cover, and the tube body is composed of an outer tube body, an inner container and a vacuum heat preservation layer; the inner container is provided with a U-shaped breaking gap at the container mouth, the gap is provided with a wedge-shaped cutting edge, and a limiting block provided with anti-skid serrations is arranged below; the container mouth is provided with a sealing flow blocking block and a rubber plug body, the plug body is provided with a medical hydrophilic liquid absorbing composite buffer layer at the bottom; and the outer tube body is provided with a real-time temperature display unit, which can adopt reversible heat-sensitive color-changing strips or waterproof miniature digital display modules.The wedge-shaped cutting edge and the limiting structure are used to realize accurate and labor-saving breaking of the swab, so that sample spatter and pollution are avoided; multi-stage sealing and liquid absorbing buffer layer double anti-overflowing can quickly absorb and inactivate leaked samples; vacuum heat preservation is combined with real-time temperature monitoring to guarantee the cold chain storage environment of the samples.The whole operation is convenient and safe, the device is suitable for clinical sampling processes, and the reliability of respiratory pathogen sample collection, preservation and transportation can be effectively improved.

The invention provides an ichthyophthirius multifilis detection reagent and detection method based on an enzymatic isothermal amplification technology, and belongs to the technical field of pathogen detection. The invention provides a primer probe group for detecting ichthyophthirius multifilis by using an enzymatic isothermal amplification (ERA) method, and a CO1 gene of the ichthyophthirius multifilis is used as a target sequence. Based on the ERA primer probe group designed by the invention, a test strip type isothermal detection kit and a fluorescent isothermal detection kit are developed, and are respectively associated with'test strip type visual detection 'and'fluorescent quantitative detection'. The two ERA detection kits developed by the invention have the advantages of strong specificity, high detection sensitivity, high detection efficiency and no need of high-temperature equipment, and can meet the requirements of on-site rapid visual detection. According to the invention, the technical blank of on-site rapid diagnosis of ichthyophthirius multifilis is filled, and a key technical support is provided for early prevention and control of aquaculture diseases.

The invention discloses a detection reagent, a kit and a detection method for pathogens of micropterus salmoides. The micropterus salmoides pathogen detection reagent comprises primers and probes for specifically amplifying an infectious spleen and kidney necrosis virus, a viral nervous necrosis virus, flavobacterium columnar, a perch rhabdovirus, a micropterus salmoides double-RNA virus, edwardsiella tarda, nocardia and a micropterus salmoides iridovirus; specific upstream and downstream primers and probes of the eight pathogens are sequences as shown in SEQ ID NO.1 to SEQ ID NO.24 in sequence. The micropterus salmoides pathogen detection reagent and method can detect eight pathogens at the same time, have the advantages of high sensitivity, high specificity, good repeatability and the like, provide reliable basis for prevention and control of the pathogens, also improve the prevention and control work efficiency, and have good application prospects. The kit is especially suitable for large-scale rapid screening and mixed infection diagnosis of aquaculture pathogens, and has important value and significance for prevention and treatment of micropterus salmoides pathogens.

This invention discloses a functionalized biomodified electrode for rapid pathogen detection and its preparation method, relating to the fields of biosensing and electrochemical detection technology. It includes a glassy carbon electrode substrate, the surface of which is sequentially modified with a reduced graphene oxide layer, an electropolymerized polydopamine film, and an electrodeposited gold nanoparticle layer, forming a composite nanomaterial modified electrode. Antibodies are captured and fixed to the electrode surface through covalent interactions. This functionalized biomodified electrode for rapid pathogen detection and its preparation method, using the same electrochemical workstation, first electropolymerizes dopamine to form a film and then electrodeposits gold nanoparticles. This allows for precise control of film thickness, gold particle size, and coverage density by parameters such as polymerization potential, scan cycles, deposition potential, and deposition time. This avoids batch-to-batch fluctuations caused by dissolved oxygen and solution disturbances in traditional chemical methods, reducing the relative standard deviation of peak current detection from different batches of electrodes from 18.7% to 4.2%–4.5%.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The invention belongs to the technical field of molecular detection, and particularly relates to a respiratory tract pathogen detection kit based on capillary electrophoresis and application of the respiratory tract pathogen detection kit, the kit can detect common pathogens which are possibly infected by respiratory tract patients and cannot be detected and determined through a conventional technology or a kit, and the kit at least has the technical advantages of being high in sensitivity and specificity.

The application discloses a primer probe composition which is composed of a forward primer, a reverse primer and a probe primer. The sequence of the forward primer is shown as SEQ ID No. 1, the sequence of the reverse primer is shown as SEQ ID No. 2, and the sequence of the probe is shown as SEQ ID No. 3. The application establishes a detection method for Pseudomonas syringae pv. actidii, which comprises the following steps: extracting genomic DNA of a sample to be detected, taking the genomic DNA as a template, and performing RAA detection by using the primer composition; and determining whether the sample to be detected contains Pseudomonas syringae pv. actidii according to a strip of a LFD test paper. The detection method provided by the application has a minimum detection sensitivity of 1 pg / µL for Pseudomonas syringae pv. actidii DNA, and has a minimum detection limit of 1×10 3 cfu / mL for a bacterial suspension, and can provide a new technical means for early diagnosis and disease prevention and control of bacterial canker disease of kiwifruit.

The utility model discloses an AIE vaginal secretion dual fluorescent staining solution preparation sampling tube, which relates to the field of gynecological pathogen detection, and comprises a sampling tube body, pressing holes are arranged below two sides of the sampling tube body, and shielding components are arranged on two sides inside the sampling tube body. By arranging the shielding assembly, when the negative pressure ball needs to be pressed through the pressing hole, a worker pulls a pull rope through a pull ring, the pull rope pulls a sliding block to move upwards, the sliding block moves upwards to extrude a spring, so that the spring is stressed and compressed, meanwhile, a baffle is driven to move upwards, the baffle moves into the containing cavity, and at the moment, the baffle does not shield the pressing hole any more; a worker can press the negative pressure ball through the pressing hole, when the negative pressure ball does not need to be pressed, the pull ring is loosened, the spring is stretched to push the sliding block to move downwards to reset, the baffle is driven to move to reset, the baffle shields the pressing hole, and the situation that the sealing plug is moved out of the suction pipe when the negative pressure ball is pressed by fingers in the sampling pipe taking process is avoided.