153 results about "Infection detection" patented technology

The invention provides a virus infection detection and identification technology based on metagenomics. The virus infection detection and identification technology based on the metagenomics comprises the four portions of sample preparation, high-throughput sequencing, bioinformatic analysis and result re-checking. In the sample preparation portion, viral nucleic acid is effectively extracted or enriched from detection samples according to the requirements of the virus infection detection and identification technology based on the metagenomics and characteristics of different types of detection samples, and a nucleic acid library which can be used for a next-generation sequencing instrument is established. In the high-throughput sequencing portion, the nucleic acid library established in the sample preparation step is sequenced so to obtain sufficient high-quality nucleic acid sequence information. In the bioinformatic analysis portion, a large number of high-quality nucleic acid sequences obtained in the high-throughput sequencing step is analyzed to further obtain viral component information prompted by the nucleic acid of the samples. In the result re-checking portion, a bioinformatic analysis result and other information, such as technical contrast, are integrated to perform comprehensive study and judgment, finally alternative infection virus is determined, and other technologies, such as PCR, are utilized to perform re-checking.

The invention discloses a blood metagenome sequencing data analysis method and device and an application thereof. The blood metagenome sequencing data analysis method comprises a data quality controlstatistics and splitting step, a humanized sequence removal step, a plasmid sequence removal and internal reference comparison step, a pathogen genome sequence library comparison step and a pathogen parameter annotation step. The invention provides a blood flow infection detection device based on a blood metagenome sequencing data analysis method. According to the method and device provided by theinvention, the free nucleic acid sequences in blood are detected through a metagenome sequencing method, a bioinformatics analysis method is combined, more than 8,000 pathogenic microorganisms such as bacteria, viruses, fungi and parasites possibly existing in a blood flow infection patient body are detected at a time, and a detection result can be obtained within 24 hours at the soonest. The speed, the sensitivity, the accuracy and the efficiency of blood flow infection detection are greatly improved.

A non-invasive, calorimetric infection detector is provided, comprised of a substrate, and one or more indicator compositions disposed upon or incorporated therein. These indicator compositions exhibit a persistent change color when exposed to gaseous oxides of nitrogen and acids formed therefrom, providing a means of detecting NO production in a wound, which has been found to occur at a high level at the onset of infection in a wound. In addition, a bandage is provided, comprised of the detector, as well as a porous portion, and preferably a hydrophobic barrier layer to protect the detector from contamination by water and other fluids draining from the wound. The non-invasive, calorimetric infection detector, and bandage containing same, can be utilized to provide a convenient, easily utilized colorimetric means of detecting the onset of wound infection, thereby enabling caregivers to effectively and timely treat infections.

A worm propagation modeling system for use with a mobile ad-hoc network (MANET) includes an infection detection module receiving temporal dynamics information relating to temporal dynamics of worm spread in the MANET and spatial dynamics information relating to spatiality of nodes in the MANET. The infection detection module detects infection in a network segment of the MANET based on the temporal dynamics information and the spatial dynamics information.

Described herein is a system that detects ransomware infection in filesystems. The system detects ransomware infection by using backup data of machines. The system detects ransomware infection in two stages. In the first stage, the system analyzes a filesystem's behavior. The filesystem's behavior can be obtained by loading the backup data and crawling the filesystem to create a filesystem metadata including information about file operations during a time interval. The filesystem determines a pattern of the file operations and compares the pattern to a normal patter to analyze the filesystem's behavior. If the filesystem's behavior is abnormal, the system proceeds to the second stage to analyze the content of the files to look for signs of encryption in the filesystem. The system combines the analysis of both stages to determine whether the filesystem is infected by ransomware.

Host malware (or change) may be detected by (1) receiving baseline set of response time information for each of one or more transactions involving (A) the host and (B) at least one peer of the host, (2) determining or receiving a later set of response time information for each of the one or more transactions involving the host and the at least one peer of the host, and (3) determining whether or not host slowdown has occurred using the baseline set of response time information and the later set of response time information. The execution of a host malware (or change) protection policy may be controlled using at least the determination of whether or not host slowdown has occurred.

Embodiments described herein relates to a platform for infection surveillance that can assist infection preventionists and antimicrobial stewardship teams with infection detection rules and dynamic alerts by aggregating and processing infection surveillance data with contextual healthcare data regarding a facility, organization, and so on. The platform can enable proactive prevention of infection risks by detecting risk patterns in the data and generating alerts.

The invention discloses a full-automatic genital tract infection detection system which comprises a plurality of joint inspection cards for detecting a sample, an automatic supply unit, an automatic card dispatching unit, an automatic sample injection unit, an automatic transmission unit, an automatic incubation unit, a reagent injection unit, an automatic measuring and reading unit and an electrical control unit, wherein the electrical control unit controls automatic running of all the units through software of a computer. According to the full-automatic genital tract infection detection system disclosed by the invention, due to the processes of automatic card dispatching, automatic sample injection, automatic incubation, automatic measuring and reading and the like which are controlled through the computer, manual participation steps are reduced, the measuring and reading accuracy is improved, and the test speed of detecting more than a set number of samples per hour can be achieved.

The invention relates to livestock epidemic disease infection and immune identification and detection instruments, and in particular relates to a piece of test paper for identifying and detecting a virulent strain and a low virulent strain of hog cholera viruses. The test paper consists of a support plate, a sample pad, a gold mark pad, a detection membrane and a water absorbing pad, wherein a virulent infection detection line T1 '|', a low virulent infection detection line T2 '|' and a quality control line C '|' are arranged on the detection membrane. When the test paper is used, three red strips '|||' mean virulent virus infection of hog cholera viruses, two red strips '||' mean vaccine immunity of hog cholera viruses, and one red strip '|' means hog cholera viruse negative. The test paper is high in specificity, high in sensitivity, wide in reaction spectrum and simple, convenient and rapid to operate, can be used for detecting conventional low virulent vaccine strains and multiple epidemic virulent strains, can be widely used for identifying and detecting hog cholera virus infection and immune, and can be easily popularized and applied in production practice.

The invention discloses a virus infection detection method. The virus infection detection method comprises the following steps of: (1) coupling a viral reactogenicity polypeptide to a non-protein polymer to obtain a coupled polypeptide; and (2) adding the coupled polypeptide in a coating liquid, then adding a serum sample (to be detected) to perform the ELISA (enzyme-linked immunosorbent assay) detection, judging the virus and subtype infection thereof according to an immunoadsorption reaction. The detection method provided by the invention can obviously improve the detection sensitivity as well as obviously reduces the non-specific background readout, has high specificity, simplicity in operation, rapidness in diagnosis speed, and is economic and convenient in large scale detection; and the detection method is a good method easy for popularization and wide in application prospect. In the detection provided by the invention, the dosage of the coating antigen can be 10 ng / ml, and the less dosage is in favor of popularization and application.

The invention relates to a strain marker for assisting COVID-19 diagnosis and application thereof and belongs to the technical field of pathogenic microorganism infection detection. The strain markercomprises at least one of haemophilus parainfluenzae, propionibacterium, veillonella atypica, aggregatibacter segnis, alloprevotellatannerae, campylobacter showae, fusobacteria, fusobacterium nucleatum, fusobacterium periodonticum, gemella sanguinis, haemophilus parahaemolyticus, haemophilus sputorum, porphyromonas gingivalis, prevotella intermedia, prevotella multifurmis, prevotella nanceiensis,prevotella nigrescens and prevotella timonensis. A prediction model ROC obtained by the strain marker has an AUC value being 0.910, and the strain marker can be used for assisting diagnosis of SARS-CoV-2 infection, so that the diagnosing performance on COVID-19 pneumonia can be improved, and the defect that viral nucleic acid detection is false negative can be overcome to a certain extent.

The invention belongs to the field of molecular biology and discloses application of a candidatus liberibacter asiaticus PAP protein antibody in preparation of a citrus yellow shoot early infection detection kit. An operating titer of a PAP protein multiclonal antibody prepared by utilizing the conventional way is determined to be 1:(500-1000) by virtue of an enzyme-linked immunosorbent assay (ELISA) method. The PAP multiclonal antibody is utilized for performing ELISA detection, and the lowest PAP protein content which can be detected is about 0.005 [mu]g. In the actual use, the prepared PAPmulticlonal antibody can be utilized and applied to ELISA detection on an HLB early infected sample, and 7 pathogenic bacteria can be detected at least.

The invention relates to the research field of biological diagnostic reagents, in particular to an ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting APP (Actinobacillus Pleuropneumoniae). The kit comprises a washing solution, a colorimetric solution, a stopping solution, a positive control specimen, a negative control specimen and an ELISA plate coating a first antibody as well as a second antibody for detection, wherein the first antibody is a monoclonal protein antibody of the APP rApxIVN, and the second antibody is a biotin-labeled monoclonal protein antibody of the APP rApxIVN. The invention can be applied to detecting APP-infected pig serum and has favorable importance and sensitivity.

The invention discloses a cache infection detection method and apparatus based on a deep analysis on a DNS message. Therefore, a DNS cache infecting attack can be detected timely and warning is carried out. Moreover, after DNS cache infection, a change of a network domain name address in the DNS server cache can be reported accurately, so that the DNS server operaton and maintennace personnel can correct the wrong network domain name address in the DNS server timely.

The invention relates to a substance, a kit and a method used for detecting systemic infection. The substance is a circulating DNA disintegrated and released in vivo from a pathogenic bacterium. The substance provided by the invention is based on the detection of DNA degraded by the pathogenic bacterium of peripheral blood, objectively reflects that whether the pathogenic bacterium invades into blood circulation, and better helps to distinguish the systemic infection and non-infective diseases. The substance does not depend on the separation and identification of a living bacterium thallus, is not affected by the content of the living bacterium in a blood specimen, is not affected by the influences of the contaminated bacterium in specimen collection process, and is higher in detection sensitivity and specificity. The provided detection kit comprises a capture probe for 16 S rDNA of 662 pathogenic bacteria, and can better identify the species of the pathogenic bacterium. The provided detection method better reflects the severity of the infection as well as the course and the condition of the disease through the semi-quantitative analysis of the circulating DNA from the bacterium in the peripheral blood, so as to guide treatment and evaluate prognosis.

Emulation software executes upon an operating system of a computer and creates an emulated computer. Bootstrapping code is read into this emulated computer from a sector (such as a master boot record) of a mass storage device. Instructions in the bootstrapping code are executed by an instruction emulator (also using an emulated CPU, emulated memory and an emulated hard disk) and these instructions and behavior are collected as each instruction executes. Access to the actual hard disk may be allowed. The collected information is then compared to a virus signature or behavior rules indicating malware and a conclusion is drawn as to whether the bootstrapping code includes malicious software.

The invention relates to a bee Israeli acute paralysis virus RT-PCR detection reagent kit and a detection method thereof. The reagent kit and the detection method are specially used for detection of bee Israeli acute paralysis viruses. The reagent kit comprises an inverse transcription premixed solution, a PCR premixed solution, reverse transcriptase enzyme, Taq DNA polymerase, positive control, negative control and RNase-free ddH2O. The reagent kit has the advantages that stability and specificity are good, high specificity is achieved in bee IAPV detection, cross reaction with other bee viruses does not exist, and repeatability is good; sensitivity is high and sensitivity can reach 103 copies; operation is easy and convenient, the reaction premixed solution is adopted, operation steps are reduced, and pollution risks are reduced. The method can be used for IAPV infection detection of bees, and plays an important role in early prevention and in-time prevention of the bee Israeli acute paralysis viruses.

The invention relates to a test strip for joint detection of a COVID-19 antigen and an antibody and application of the test strip. The COVID-19 antigen and the antibody are detected at one time by adopting colloidal gold immunochromatography, and detection results of the COVID-19 antigen, IgM and IgG can be distinguished. Nasopharyngeal swab secreta and serum or plasma or whole blood or end tip blood are simultaneously subjected to sample adding; antigen-antibody combined detection is realized on a single test strip at one time; the 'window period 'of virus infection detection is prolonged; the cost and time are saved; the detection window time is advanced; early discovery and early isolation are realized; and the test strip has very important significance for screening potential infectious crowds.

The invention discloses a diagnostic marker and application of the diagnostic marker in COVID-19 diagnosis and coronavirus previous infection detection. The diagnostic marker comprises a peptide fragment COVID19-V001, the amino acid sequence of the peptide fragment COVID19-V001 is a sequence containing five or more continuous amino acids in FKEELDKYFKNH, or the amino acid sequence of the peptide fragment COVID19-V001 is a sequence formed by substituting or / and deleting or / and adding one or more amino acids in FKEELDKYFKNH. Based on the diagnostic marker provided by the invention, an indirect method is adopted to qualitatively detect the level of the IgG antibody of the anti-peptide fragment in human serum. A detection kit established on the basis of the diagnostic marker can be used as anauxiliary means for diagnosing the coronavirus disease 2019 (COVID-19).

The invention discloses a pathogenic microorganism detection system and method based on nanopore sequencing, and belongs to the technical field of microorganism detection. The pathogenic microorganism detection system comprises: a data input module; a database storage module; a pathogenic microorganism identification module; and a result output module. By utilizing the system and the method provided by the invention, pathogens such as bacteria, fungi, viruses, parasites and the like in a sample can be rapidly analyzed. The system and method hav the characteristics of long sequencing reading length, rapid and accurate sequence reading and the like, and can meet the clinical infection POCT detection requirements. Meanwhile, due to the characteristic of long length, the toxicity and drug-resistant genes corresponding to the pathogens can be analyzed through sequencing data, and clinical infection diagnosis and treatment can be effectively assisted.

The invention relates to the technical field of molecular biology and clinical detection, in particular to a primer probe group for detecting a mycobacterium tuberculosis complex and rpoB mutations based on a multi-enzyme isothermal rapid amplification technology and application of the primer probe group. A 1296th-site base mutation of an rpoB gene is used as a molecular marker for identifying a mycobacterium tuberculosis complex strain, and a specific primer probe group is designed aiming at amino acid mutations of the 1296th-site and a 516th site, a 526th site and a 531st site of the rpoB; amethod for detecting the mycobacterium tuberculosis complex strain and the rpoB mutations based on a multi-enzyme isothermal rapid amplification technology is developed. The primer probe group disclosed by the invention is high in detection sensitivity and strong in specificity; the method has the advantages of low dependence on equipment, short detection time, realization of rapid and accurate detection, realization of visual detection in combination with a lateral flow test strip, and important application values for the infection detection of the Mycobacterium tuberculosis complex strain and the drug resistance detection of Mycobacterium tuberculosis.

The present invention is high risk human papilomavirus (HPV) detecting process, which includes the following steps: 1. designing and synthesizing proper specific primer, and amplifying the target DNA of the detected sample specifically for the second time by means of one-step nest PCR technology; and 2. hybridizing and selectively typing the target DNA of the detected sample through twice specific selective amplification and the specific segment of high risk HPV by means of combination to low density DNA chip technology, and signal identification through fluorescent detecting or direct color developing. The present invention provides also one corresponding kit. The present invention can amplify specific target DNA segments of 13 kinds of HPV simultaneously and detect several kinds of virus subtypes of HPV through once reaction, and has the advantages of high speed, high efficiency, high sensitivity, high specificity, etc.

The invention relates to a primer and a probe for real-time fluorescence quantification PCR detection of duck II hepatitis A virus and belongs to the field of epizootiology. The invention includes design of specific primer and probe sequences, construction of standard plasmids, establishment and optimization for a real-time fluorescence quantification PCR amplification method, and detection and judgement for results. The method for real-time fluorescence quantification PCR detection of duck II hepatitis A virus established by the invention has high sensitivity, high stability, high specificityand high repeatability for detecting duck II hepatitis A virus, is capable of detecting at least 33.7 copies and can be used for detecting duck II hepatitis A virus infection in a clinical sample.

The invention discloses a hospital infection monitoring management device and method, and the device comprises a hospital infection monitoring management server, a hospital information integration platform, a hospital information system, a laboratory information system, a medical image archiving and communication system, an electronic medical record system, a hospital infection detection server, aWeChat enterprise number server, and a smart phone. Through the functions of new media information push, image-text propaganda and education and questionnaire surveying, the system achieves the closed-loop hospital infection overall flow management of Plan, Do, Check and Action. The system improves the suspected hospital infection detection accuracy, reduces the workload of a doctor, optimizes the hospital infection monitoring management flow, and can be used for the hospital infection monitoring.

The present invention is an early infection detection system comprising: a housing having a sensor system section and tray section; an inflow port in fluid communications with a catheter to allow bodily fluid to flow from a patient into the tray section; a color sensor included in the sensor section and in electrical communications with a control module configured to sense the color of a reagent and convert the color to a numeric value representing the amount of a compound, such as bacteria, present in the bodily fluid; a tray carrying a reagent that darkens relative to the amount of the compound present in a bodily fluid that contacts the strip; and, computer readable instructions included in the control module for receiving the numeric value and transmitting the numeric value to a local server.

A fungus fluorescence staining liquid is disclosed and comprises an A staining liquid and a B staining liquid. The A staining liquid includes 0.03-0.1 part by weight of fluorescein, 1-5 parts by weight of a cleaning agent and 80-150 parts by weight of water. The B staining liquid includes 0.3-1 part by weight of dye and 80-150 parts by weight of water. The A staining liquid can combine cell wall beta-polysaccharides of various funguses, thus labeling and fluorescing for detection. The B staining liquid is adopted as a counterstaining liquid and used for reducing the fluorescence background. The fungus fluorescence staining liquid comprising the A staining liquid and the B staining liquid is a rapid fungal infection detection product and can combine various funguses to emit fluorescence, thus detecting whether various suspected fungal infection diseases are fungal infection or not.

Methods of determining the quality of milk due to the presence of mastitis, by monitoring the concentration of various ions, and ratios of those ions, in the milk. As the level of mastitic infection progresses, the concentrations of sodium and chloride ions increase and the concentrations of potassium and calcium ions decrease. A ratio of sodium to potassium is more sensitive to infection detection than either ion concentration alone. Similarly, sodium to calcium, chloride to potassium and chloride to calcium is more sensitive than any of the ions alone.

The invention discloses a preparation method of AGP detection antigen used for diagnosing Marek's virus infection, relating to the poultry disease detection reagent field. The method comprises the steps as follows: firstly, the separating effect experimental evaluation is carried out on the target virus antigen, so that a permeation-typed filter membrane element and a retention-typed filter membrane element are screened out from ultrafiltration membrane elements with different molecular weight cutoff; secondly, the permeation-typed filter membrane element and the retention-typed filter membrane element which are screened out are respectively used to purify and concentrate the liquid containing the target virus antigen; finally, the purified and concentrated solution is detected and quantified, so that the Marek's virus infection AGP detection antigen is prepared; and no reagent is added in the process of ultrafiltration treatment, therefore, not only neither secondary pollution nor contamination is produced in the production process, but also the emission liquid is actually further purified, so as to reduce the pollution to the environment and improve resource utilization, thereby really achieving the purpose of transforming trash into treasure, and the preparation method also has the advantages of no phase state change, mild conditions, simple operation, low cost and high recovery rate, etc.

The invention discloses a new HIV infection detection kit and a preparation method, and relates to the technical field of biology. According to thenew HIV infection detection kit, a one-step enzyme reaction method is used, detection time is greatly shortened, sensitivity is high, specificity is high, and stability is good; andall three antigens of B, E, and D of HIV subtypesin China can be simultaneously detected, and the detection accuracy rate of a new HIV infection is increased. Asecondary coating method is used for coatingan enzyme-linked plate, the amount of adetection raw material is greatly decreased, and indirect labeling is used for improving the activity, reaction consistency, and accuracy rate of the antigen; and detection of trace samples is facilitated, steric hindrance is reduced, and the reaction efficiency, sensitivity, and specificity are improved.