103 results about "Infection diagnosis" patented technology

The invention discloses a rice leaf blast detection and classification method based on the multi-spectral image processing. A visible/near infrared multi-spectra camera is used for real-timely collecting monochromatic gray images of three waveband channels of green light wavebands, red light wavebands and near infrared wavebands, and then Matlab software is used for programming application software by an image processing method for image processing. The method comprises the removal of background, noises, interferences, and the like, and the recognition analysis of crop disease plague information, and the accurate and rapid processing of whether crops are ill and the position and the classification of disease plagues are realized. The disease recognition time of each picture is only a few seconds. The rice leaf blast detection and classification method based on the multi-spectral image processing is used for the rapid, accurate, stable, real-time and non-destructive crop leaf blast infection diagnosis and for accurately indicating the position of the disease plagues and the classification of infection grades, reducing the dosage due to the overall spraying, lowering the production cost, reducing pollution, providing data support for the variable spraying and improving the decision level of the accurate spraying. The method plays an active role in realizing the precision agriculture.

The invention relates to an ELISpot tuberculosis infection diagnostic kit and the application, which adopts the genetic engineering technology to obtain a CFP10-ESAT6 fusion protein antigen from a mycobacterium tuberculosis by cloning, expression and purification, a tuberculosis specific cell stimulator is used for stimulating the peripheral blood T lymphocyte cell of the detected person to secrete a specific IFN-Gamma, then the invention is detected by ELISpot, and the whole process needs to take two days. The usage of the invention can be used for detecting whether the detected person is infected by mycobacterium tuberculosis by using the naked eye or instrument to determine the result according to the number of the generated purple blue spots, so as to assist the diagnosis of tuberculosis and differential diagnosis.

The present invention relates to a method for in vivo detection of a biofilm infection residing in a mammal, the method comprising (i) administering to the mammal a diagnostic-effective amount of a biofilm-specific probe, wherein the probe comprises a bio film-targeting moiety and a paramagnetic nanoparticle core; and (ii) imaging the mammal to detect the presence of the biofilm infection by observing the mammal using a magnetic resonance diagnostic technique after the biofilm-specific probe has been provided sufficient time to selectively bind to the bio film infection that may be present in the mammal. The invention also relates to methods of treatment of a bio film infection, and compositions and kits useful in the detection and/or treatment of bio film infections.

The invention discloses a test paper for rapidly detecting swine fever antibody and a preparation method thereof. The inventive test paper consists of a sample pad (4), a glass fiber member (3), a cellulose nitrate membrane (2), a water absorbent pad (1) and a support (5); wherein the cellulose nitrate membrane contains a detection line (6) coated by swine fever virus E2 protein and a reference line (7) coated with rabbit anti swine fever antibody; and the glass fiber member is combined with swine fever virus E2 protein marked by colloidal gold. The test paper can rapidly detect possibly present swine fever antibody in a sample to achieve rapid detection and timely epidemic control, thus creating favorable conditions for further separation and identification. The test paper has the advantages of convenient, rapid and easy usage, clear result, easy generalization, and no need for special instrument and equipment as well as professionals, and is suitable for large-batch onsite detection for base layer and for sudden events. The test paper is suitable for epidemic inquisition and performs assistant effect on swine fever virus infection diagnosis.

The invention belongs to the field of biomedicine examination, and relates to an MTB (Mycobacterium Tuberculosis) infection diagnosis kit. The MTB infection diagnosis kit provided by the invention is prepared from the following components: an antigen stimulant, a pre-coated ELISPOT (Enzyme-Linked Immunospot Assay) plate of a capture antibody, an insulin-like growth factor, a detection antibody, HRP (Horse Radish Peroxidase)-labeled streptavidin, a 3-amino-9-ethyl carbazole developing solution, antibody diluent and a positive control stimulant; the antigen stimulant is selected from one or multiple polypeptides in sequences as shown in SEQ ID NO.1 to 12. The MTB infection diagnosis kit provided by the invention is high in sensitivity and specificity and stable in property; meanwhile, when the MTB infection diagnosis kit provided by the invention is applied to in-vitro detection on MTB, the detection time can be saved, the detection steps can be simplified, and the detection efficiency can be increased.

The invention provides a hepatitis B microRNA molecular marker composition and application thereof in preparing a hepatitis B infection diagnosis and/or prognosis evaluation kit. The hepatitis B microRNA molecular marker composition comprises more than one microRNA molecule as shown in SEQ NO. 1-18. The invention also provides a diagnostic kit for instructing the infection diagnosis and/or prognosis evaluation of hepatitis B. The hepatitis B microRNA molecular marker and the diagnostic kit which are provided by the invention have the characteristics of easiness for operation, safety, no injury, high specificity, high sensitivity and easiness for large-scale screening in instructing the infection diagnosis and/or prognosis evaluation of a hepatitis B patient.

The invention discloses nanoparticles, a preparation method and an application thereof and a medicament, and relates to the technical field of nanoparticles. The nanoparticles comprise a gold nanorodinner core and a silver shell layer covering the outer surface of the gold nanorod, wherein the aspect ratio of the gold nanorod is 4-8, and Au/Ag/Au nanorods or Au/Ag/Au nanostars can be further prepared. The LSPR maximum absorption peak wavelength of the nanoparticles falls in the near infrared-II, photothermal conversion efficiency is higher than that of the near infrared-I, the photothermal treatment effect is good, and ablation of bacterial cells is further facilitated. Photoacoustic imaging has higher tissue penetration depth, long time and high spatial resolution. The antibacterial effect of Ag<+> released by an external Ag shell and photo-thermal sterilization effect of internal Au cooperate to achieve higher sterilization efficiency. The nanoparticles can be used for photoacousticimaging and bacterial infection diagnosis and treatment agents, and can non-invasively monitor local release and photothermal treatment processes of Ag<+>.

Group C rotaviruses are a cause of acute gastroenteritis in children and adults that is distinct from group A RV. However, human group C rotaviruses cannot be grown in culture, resulting in a lack of tools for detection and treatment of GrpC RV disease. Consequently, the burden of GpC RV disease has not been clearly established. Isolated recombinant human rotavirus group C virus-like particles are provided according to embodiments of the present invention along with methods of their production and use in, inter alia, detection of Grp C RV infection, diagnostic assays and immunogenic compositions.

The invention discloses a biomarker for diagnosing mycobacterium tuberculosis infection. The biomarker comprises IL-2 and IL-10. The invention proves that cell factors IL-2 and IL-10 can serve as markers for tuberculosis infection diagnosis for the first time, the tuberculosis infection can be diagnosed by measuring the concentration of the IL-2 and the IL-10 after peripheral blood monouclear cells are stimulated by tuberculosis antigen, and active tuberculosis and latent tuberculosis infection are further distinguished. A tuberculosis infection blood detection reagent and a kit which are prepared on the basis of the two cell factors provide new technological basis for quick diagnosis of the active tuberculosis, have the advantages of high sensitivity, high specificity and the like, are quick in operation, reasonable and practical, and can obviously improve the diagnosis efficiency of the active tuberculosis.

A sensitive, multicomponent diagnostic test for infection with T. cruzi, the causative agent of Chagas disease, including methods of making and methods of use. Also provided is a method for screening T. cruzi polypeptides to identify antigenic polypeptides for inclusion as components of the diagnostic test, as well as compositions containing antigenic T. cruzi polypeptides.

The invention discloses an African swine fever virus (ASFV) antigen detection fluorescent microsphere immunoassay test strip, which comprises a PVC bottom plate. A sample pad, a fluorescent pad, a nitrocellulose membrane and an absorbent pad are sequentially fixed on the PVC bottom plate; the fluorescent pad contains a monoclonal antibody Mab1 fluorescently labeled with an epitope of an anti-ASFVp30 antigen; the surface of the nitrocellulose membrane is marked with a detection line and a quality control line, the detection line is a monoclonal antibody Mab2 for another epitope of the ASFV p30antigen, and the quality control line is a goat anti-mouse IgG antibody. The test strip is suitable for detection on the ASFV p30 antigen in peripheral blood, serum, saliva and tissue (lung and spleen) polishing slurry of infected pigs, ASFV infection is diagnosed in a short time, and the test strip is particularly suitable for on-site ASFV infection diagnosis, epidemiological investigation and international trade quarantine inspection of pigs; and a result is directly displayed in collaboration with an immunofluorescence analyzer, the operation is quick and simple, the accuracy is high, andthe practicability is strong.

The difficult problem of the contamination of cell cultured mycoplasma and the infection diagnosis of various mycoplasma urgently need a reliable and effective kit. Mycoplasma culture by a fluorescent staining method is reliable but takes time and trouble, and culture pollution is intensified. A direct PRC method for amplifying a mycoplasma gene, which is used for the routine detection of the mycoplasma is easy for cross contamination, and high false masculinity is only used as an auxiliary reference. The invention relates to a mycoplasma conservative gene which across links a plant arabidopsis LEY sequence into a reporting gene DNA by a hybridization probe and amplifies the indirect detection of a reporting gene. The mycoplasma conservative gene is characterized in that mycoplasma 16sRNA is hybridized with the head part and the tail part of the LEY reporting gene DNA whose head part and tail part are provided with mycoplasma conservative sequence probes; the reporting gene DNA is catalyzed into a ring by heat-proof ligase, and the annular reporting gene is reversely amplified by PCR so as to indirectly reflect the detection of the mycoplasma; by adjusting ligase reaction, the reporting gene is difficultly across linked by the cross contamination, thereby reducing the false masculinity; and if a system is contaminated, the reporting gene can be changed.

The invention provides COVID-19 and delta mutant strain detection kit and a detection method thereof, and belongs to the technical field of molecular biological detection. A series of primer probe groups are redesigned, and detection targets are increased, so that the novel coronavirus wild type and delta mutant strains are effectively distinguished. The kit can be used for in-vitro qualitative detection of COVID-19 or delta mutant strain infected pneumonia suspected cases and suspected aggregation case patients, and other patients needing COVID-19 infection diagnosis or identification of COVID-19 genes in nasopharyngeal swab, sputum and other samples of diagnosers.

The invention discloses a detection test paper. The test paper comprises (1) a reaction support, (2) a water absorption pad, (3) a nitrocellulose membrane and (4) a gold-labeled antibody protective membrane, wherein the nitrocellulose membrane is coated with a detection strip and a quality control strip of a rabbit anti-recombinant abrin A-link antibody and a quality control antibody; and the gold-labeled antibody protective membrane contains the rabbit anti-recombinant abrin A-link antibody labeled by colloidal gold. The test paper can be matched with a small instrument to carry out semi-quantitative detection, does not need professional training, has clear and identifiable result, can objectively store data, is simple to operate, easy to popularize, suitable for a basic level, suitable for field detection of emergency and suitable for epidemiological investigation, and plays an auxiliary role in diagnosis of abrin infection.

The invention provides a test strip for rapid detection of Japanese encephalitis virus IgM antibody. A Japanese encephalitis virus E gene antigen domain III and a double-antibody IgM III coat a nitrate cellulose film (NC film), and a membrane chromatography indirect sandwich method is adopted to detect the Japanese encephalitis virus specific IgM antibody in an infected human body specimen in combination with a colloidal gold labeled antihuman IgM monoclonal antibody. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of Japanese encephalitis virus infection.

The invention discloses a piece of detection test paper which comprises (1) a reaction support; (2) an absorbent pad; (3) a nitric acid fiber membrane which is coated with a detection strip of a bacillus anthracis antibody and a quality control strip of a quality control antibody; and (4) a GAB gold-labelled antibody protective film which comprises the bacillus anthracis antibody marked with colloidal gold. The test paper of the invention can be coordinated with small instrument for semi-quantitative detection, does not need professional training, has clear and easily-identified result, can objectively preserve data, operates simply, is easy for promotion, can be suitable for the grass-roots units, the on-site detection of unexpected events, and the investigation of epidemiology, and plays a supporting role for the infection diagnosis of the bacillus anthracis.

The invention discloses a joint prosthesis infection diagnosis kit. The joint prosthesis infection diagnosis kit comprises primers, a magnesium-ion-free PCR reaction buffer, MgCl2, Taq polymerase, a dNTP mixture and ddH2O, wherein the primers comprise a universal primer, a specificity primer and an internal reference primer; the specificity primer consists of the following compositions: an ica D gene detection primer, an eno gene detection primer, a sar A gene detection primer, an agr gene detection primer, a Mec A gene detection primer and a Mre B gene detection primer. The joint prosthesis infection diagnosis kit is high in joint prosthesis infection diagnosis, particularly early diagnosis, the detection accuracy is high, infection can be detected rapidly and accurately in the early period when the joint prosthesis infection does not have clinical symptoms, therapeutic measures can be taken in time, and further deterioration of the state of an illness is prevented.

The invention provides a hepatitis b /hepatic cirrhosis microRNA molecular marker combination, which comprises more than one micro RNA nucleic acid molecules selected from mir.146a-5p, miR-22-3P and miR-381.3P, and a purpose of the marker combination for preparing hepatitis b/hepatic cirrhosis infection diagnosis and/or prognosis assessment kit. Due to a significant difference between content of a microRNA molecular marker of a hepatitis b/hepatic cirrhosis patient in plasma/serum and content in plasma/serum in health control, the hepatitis b/hepatic cirrhosis patient can be effectively distinguished from people of the health control; a chronic hepatitis b/hepatic cirrhosis disease state can be comprehensively analyzed, which can be used for guiding the patient treatment and usage of medication. The invention also provides a diagnostic kit for guiding the hepatitis b/hepatic cirrhosis infection diagnosis and/or prognosis assessment. By using the hepatitis b/hepatic cirrhosis microRNA molecular marker and the diagnostic kit, infection diagnosis and/or prognosis assessment of the hepatitis b/hepatic cirrhosis patient can be guided, and the combination has the characteristics of simple operation, safety and no wound, high specificity, high sensitivity and easy massive screening.

The invention provides a fast detection testing strip for detecting coxsackie virus A16 (CA16), which is monoclonal antibody or polyclonal antibody and quality control anti-mouse IgG of a nitric acid fiber membrane (NC membrane) coated with coxsackie virus A16 (CA16) specific antigen VP1. The monoclonal antibody combined with coxsackie virus A16 (CA16) specific antigen VP1 labeled by colloidal gold is used for detecting the coxsackie virus A16 (CA16) in the sample by using a membrane chromatography double antibody sandwich method. The application of the detection testing strip has the advantages of simple operation, convenience, rapidity, no special apparatus requirement, no professional training requirement, clear results, easy distinction and easy popularization, thereby the detection testing strip is suitable for basic level, large batch on-site detection for the emergent events and diagnosis at early stage, and has auxiliary effect to the infection diagnosis of coxsackie virus A16 (CA16).