74 results about "IgM.monoclonal" patented technology

The invention discloses a novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2 structural protein-marker and a goat anti-rabbit IgG-marker, and the nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human IgM monoclonal antibody and a quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a human body, assists a nucleic acid detection means, and provides powerful support for epidemic situations.

The invention relates to an ELISA (enzyme-linked immuno sorbent assay) detection kit of a porcine pseudorabies virus IgM antibody, and a preparation method and application thereof. The ELISA detection kit of the porcine pseudorabies virus IgM antibody disclosed by the invention comprises an elisa plate of enveloping an IgM monoclonal antibody, sealing liquid, sample diluting liquid, detecting antigen, enzyme conjugate, concentrated scrubbing solution, enzyme substrate A solution, enzyme substrate B solution and stop buffer; the detecting antigen is prepared from purified porcine pseudorabies virus gE protein, structure protein gB and structure protein gD; and the enzyme conjugate is horse radish peroxidase-anti-gE, gB or gD protein enzyme compound. The specificity of the kit disclosed by the invention can be up to 100%; the sensitivity is 1:640; and the kit can be used for early diagnosis of porcine pseudorabies virus infection.

The invention relates to the technical field of biological detection, in particular to a detection method of hemorrhagic fever with renal syndrome IgM (immunoglobulin M) antibodies and a reagent kit. The detection method comprises the steps that hemorrhagic fever with renal syndrome antigens are fixed on a membrane; to-be-detected serum is added; the hemorrhagic fever with renal syndrome IgM antibodies in the to-be-detected serum are combined with the antigens fixed on the membrane; gold labeled operating fluid containing mouse anti-human IgM monoclonal antibodies is added; the mouse anti-human IgM monoclonal antibodies are combinec with the hemorrhagic fever with renal syndrome IgM antibodies in the to-be-detected serum; a red color is shown; scrubbing liquid is added finally; and the redundant gold labeled operating fluid and other impurities are washed out. The reagent kit has the characteristics of longer quality guarantee period, less cross reaction, better color rendering performance and the like.

The invention relates to an ELISA (enzyme-linked immuno sorbent assay) detection kit for porcine parvovirus IgM (immunoglobulin m) antibodies as well as a preparation method and application of the ELISA detection kit. The ELISA detection kit for the porcine parvovirus IgM antibodies comprises an ELISA plate coating a porcine IgM monoclonal antibody, sealing fluid, a sample diluent, a detection antigen, an enzyme conjugate, a concentrated cleaning solution, a zymolyte solution A, a zymolyte solution B and a stop solution, wherein the detection antigen is a purified porcine parvovirus, and the enzyme conjugate is a horse-radish peroxide enzyme-anti-PPV (porcine parvovirus) antibody enzyme conjugate. The specificity of the ELISA detection kit provided by the invention reaches 100% and the sensitivity of the ELISA detection kit reaches up to 1:800, so that the ELISA detection kit can be used for performing early diagnosis on PPV infection of a swinery.

The invention discloses a colloidal gold detection kit for hepatitis A virus IgM antibody, comprising a recombinant antigen HAV-Ag coated on a nitrocellulose membrane detection line, a goat anti-mouse IgG antibody and a gold label coated on a quality control line The mouse anti-human IgM monoclonal antibody labeled with colloidal gold coated on the pad, the recombinant hepatitis A virus antigen concentration was greater than 2mg / ml, and was determined by SDS-PAGE; the goat anti-mouse IgG antibody concentration was greater than 4mg / ml; The concentration of the mouse anti-human IgM monoclonal antibody was greater than 2 mg / ml, which was determined by SDS-PAGE, and the loading amount was two bands under the condition of 10 μl. The beneficial effects of the invention are as follows: the hepatitis A virus IgM antibody detection kit has the characteristics of rapidity, simplicity, accuracy and high sensitivity, and the whole operation time only takes 20 minutes to interpret the results. The colloidal gold rapid detection test strip is based on the multi-epitope recombinant antigen as the raw material. It has the characteristics of easier operation, low cost, good specificity and high sensitivity. It can be detected in a single batch and is easy to popularize. The detection and control effect is obvious.

The invention discloses an AB / CDE blood type grouping reagent card. The reagent card is provided with eight microcolumn gel tubes, namely a gel tube containing an immune globulin (IgM) monoclonal anti-A antibody, a gel tube containing an IgM monoclonal anti-B antibody, a gel tube containing an IgM monoclonal anti-D antibody, a gel tube containing an IgM monoclonal anti-C antibody, a gel tube containing an IgM monoclonal anti-E antibody, a gel tube which is used for negative control and contains a gel buffer solution and two gel tubes which are used for reverse typing and contain gel buffer solutions. The invention provides standard for the ABO-CDE blood type grouping; and the ABO-CDE blood type grouping reagent card which conforms to standard can be used in all hospitals and blood transfusion services to accurately group ABO-CDE blood types, so conditions are created for guaranteeing transfusion safety.

The invention provides a rapid test strip for detecting an enterovirus 71 (EV71) IgM antibody. In the invention, EV71 gene antigenic vascular adhesion protein-1 (VAP1) and anti-mouse IgG are coated on a nitrocellulose (NC) membrane in combination with a colloidal gold labeled anti-human IgM monoclonal antibody, and the specific EV71 IgM antibody in an infected human specimen is detected by utilizing a membrane chromatography capture method. The rapid test strip provided by the invention has the advantages of being simple, convenient and rapid in operation, having no need of special instrumentand equipment and professional training, ensuring clear and easily-distinguished results and being easy for popularization; and the rapid test strip is applicable to a basic level as well as field test and early diagnosis, and plays a role in assisting the EV71 infection diagnosis.

The invention discloses an AB / Rh blood type grouping reagent card and a preparation method thereof. The reagent card is provided with at least one group of microcolumn gel tubes; each group of microcolumn gel tubes comprise four microcolumn gel tubes, namely a gel tube containing an immune globulin (IgM) monoclonal A-resistant antibody, a gel tube containing an IgM monoclonal B-resistant antibody, a gel tube containing an IgM monoclonal D-resistant antibody and a gel tube which is used for negative control and contains a gel buffer solution. The invention provides standard for the ABO / Rh(D) blood type grouping; and the ABO / Rh(D) blood type grouping reagent card which conforms to standard can be used in all hospitals and blood transfusion services to accurately group ABO / Rh(D) blood types, so conditions are created for guaranteeing transfusion safety.