17 results about "IgM.monoclonal" patented technology

The invention relates to the technical fields of gene engineering, antibody engineering and immunodiagnosis. The invention provides a recombinant humanized anti-Cpn IgM monoclonal antibody. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5; the heavy chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences of the three complementary determining regions are shown as SEQ ID NO.6, SEQ ID NO.7 and SEQ ID NO.8 respectively. The invention also provides a method for preparing the monoclonal antibody. The recombinant humanized anti-Cpn IgM monoclonal antibody provided by the invention is expressed in mammalian cells by utilizing a gene recombination technology, has the characteristics of high purity, small batch difference and the like, and can be applied to a Cpn IgM antibody detection kit as a quality control product; as a quality control product, the recombinant humanized anti-Cpn IgM monoclonal antibody has the advantages of high sensitivity, high safety, inter-batch stability and the like.

The present application relates to the technical field of in vitro diagnosis, in particular to a kit and a preparation process thereof, the present application is a recombinant virus antigen of coxsackie virus A16 and EV71, and is used for specific detection of coxsackie virus A16 IgM, a magnetic micro-particle chemiluminescence kit, which comprises magnetic particles coated with mouse anti-human IgM monoclonal antibody, coxsackie virus A16 antigen labeled with horseradish peroxidase and coxsackie virus EV71 recombinant virus antigen. The reagent has excellent sensitivity, specificity and precision, and can distinguish the two pathogens of EV71 and coxsackie A16 in hand-foot-mouth disease, has high repeatability between tests, and is easy to control the standard.

The invention discloses a pearl gentian grouper IgM monoclonal antibody, which is characterized in that screened pearl gentian grouper hybridoma No. 41 is sequenced to obtain nucleotide and amino acid sequences of variable regions of a heavy chain and a light chain of the targeted pearl gentian grouper IgM monoclonal antibody respectively shown as SEQ ID NO.1-12, the heavy chain subtype of the monoclonal antibody is IgG type, the light chain is kappa type, and the light chain subtype of the monoclonal antibody is kappa type. Through codon optimization, recombinant pATX1 vectors of heavy-chain and light-chain antibodies are respectively constructed with a constant region sequence of a murine antibody. The Epinephelus lanceolatus IgM monoclonal antibody is expressed through co-transfection XtenCHO cell in-vitro culture, the chimeric immune globulin is good in reaction characteristic and high in titer (512000 or above) and is easier to store compared with a hybridoma cell mode, and the risk of losing the specific antibody is avoided.

The invention discloses an anti-zebra fish IgM monoclonal antibody and preparation and application thereof, and belongs to the technical field of monoclonal antibodies, and the anti-zebra fish IgM monoclonal antibody is secreted by a hybridoma cell strain with the preservation number of CCTCC NO: C202542. The anti-zebrafish IgM monoclonal antibody provided by the invention not only can specifically recognize IgM in serum and IgM + B cells in tissues, but also can be used for evaluating the immune effect of vaccines, is wide in application, and has positive significance on scleroderma immunity and vaccine research.

The invention discloses an anti-tilapia mossambica IgM monoclonal antibody, a hybridoma cell strain and application thereof. The CDR sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1-3, and the CDR sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.4-6; the monoclonal antibody can be secreted by a hybridoma cell strain with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2023153. The monoclonal antibody provided by the invention can be used for Western blot and ELISA specific detection of tilapia mossambica secretion form IgM, can be used for flow cytometry identification of tilapia mossambica IgM + B cells, has the characteristics of good specificity, high sensitivity, strong affinity and the like, can be used for structural analysis, immune response level detection and the like of tilapia mossambica IgM, and can be used for preparing a tilapia mossambica IgM immunoassay kit. And a foundation is laid for deep research of a tilapia immune system and establishment of a tilapia vaccine immune effect evaluation method taking the antibody level as an index.

The present application relates to the technical field of in vitro diagnosis, in particular to a composition and a kit, and provides a magnetic microparticle chemiluminescence kit for detecting coxsackievirus A10 IgM, and further provides a recombinant antigen EV71, CA16 and CA6 combination for blocking cross infection and improving the specific detection rate of CA10, which comprises magnetic particles coated with mouse anti-human IgM monoclonal antibody, horseradish peroxidase-labeled coxsackievirus A10 antigen, horseradish peroxidase-labeled coxsackievirus EV71 type, A16 type and A6 type combined blocking antigen. The kit provided by the present application has high sensitivity and specificity, and can distinguish EV71 type, CA16 type and CA6 infection cross in hand-foot-mouth disease, specifically detect A10 pathogens, and has high repeatability and simple operation.

The invention discloses a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting IgM of cynoglossus semilaevis. The double-antibody sandwich ELISA kit is constructed by taking anti-cynoglossus semilaevis IgM monoclonal antibodies secreted by a hybridoma cell strain CsIgM-12 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.46591 and a hybridoma cell strain CsIgM-39 with the preservation number of CGMCC No.46592 as a capture antibody and a detection antibody respectively. The titers of the two monoclonal antibodies are 128000 or above, the kit is good in specificity and high in sensitivity, the minimum detection concentration of the kit is 0.01 microgram / mL, the IgM content in the serum of the cynoglossus semilaevis can be accurately detected, and the kit has important significance for evaluating the health condition and immune function of the cynoglossus semilaevis.

This invention discloses a high-affinity mouse anti-eel IgM monoclonal antibody, a hybridoma cell line, and its applications. The mouse anti-eel IgM monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The CDR region of the heavy chain variable region contains three sequences as shown in SEQ ID NO. 1-3, and the CDR region of the light chain variable region contains three sequences as shown in SEQ ID NO. 4-6. The mouse anti-eel IgM monoclonal antibody can be secreted by the hybridoma cell line ArIgMH-33G9D2G7C12, with accession number CCTCC NO: C202545. The mouse anti-eel IgM monoclonal antibody provided by this invention can be used for flow cytometry to identify eel IgM. + This monoclonal antibody, developed for B cells, can be used for the specific detection of secretory IgM in eels via ELISA and Western blot. It exhibits high specificity, sensitivity, and affinity, making it suitable for structural analysis of eel IgM and detection of immune response levels. This lays the foundation for in-depth research on the eel immune system and the establishment of methods for evaluating vaccine efficacy using antibody levels as an indicator.

The invention discloses a high-affinity mouse anti-eel IgM monoclonal antibody, a hybridoma cell strain and application, the mouse anti-eel IgM monoclonal antibody comprises a heavy chain variable region and a light chain variable region, a CDR region of the heavy chain variable region comprises three sequences shown as SEQ ID NO.1-3, and a CDR region of the light chain variable region comprises three sequences shown as SEQ ID NO.4-6; the mouse anti-eel IgM monoclonal antibody can be secreted by a hybridoma cell strain ArIgMH-33G9D2G7C12, and the preservation number of the mouse anti-eel IgM monoclonal antibody is CCTCC (China Center For Type Culture Collection) NO: C202545. The mouse anti-eel IgM monoclonal antibody provided by the invention can be used for identifying eel IgM + B cells by flow cytometry, and can be used for specific detection of eel secretion type IgM by ELISA (enzyme-linked immuno sorbent assay) and Western blot. The monoclonal antibody is good in specificity, high in sensitivity and strong in affinity, can be used for structural analysis, immune response level detection and the like of eel IgM, and lays a foundation for deep research of an eel immune system and establishment of a vaccine immune effect evaluation method taking the antibody level as an index.

The invention discloses a time-resolved fluorescence immunochromatography test strip for detecting IgM (immunoglobulin M) of cynoglossus semilaevis, which is characterized in that an anti-IgM monoclonal antibody of cynoglossus semilaevis secreted by a hybridoma cell strain CsIgM-39 marked by time-resolved fluorescent microspheres is used as a detection marking antibody on a combination pad; an anti-cynoglossus semilaevis IgM monoclonal antibody secreted by a hybridoma cell strain CsIgM-12 is used as a detection line (T line) coating antibody, so that the time-resolved fluorescence immunochromatography test strip for detecting the cynoglossus semilaevis IgM is constructed. The titers of the two monoclonal antibodies are 128000 or above, the test strip is good in specificity, high in sensitivity, strong in repeatability and convenient and fast to operate, the detection time is only 10 minutes, the detection sensitivity can reach 0.00625 mu g / mL, the IgM content in serum and plasma of the cynoglossus semilaevis can be accurately detected, and the test strip has important significance for evaluating the health condition and immune function of the cynoglossus semilaevis.

The invention discloses a cynoglossus semilaevis IgM monoclonal antibody, which is characterized in that screened cynoglossus semilaevis hybridoma No.39 is sequenced to obtain nucleotide and amino acid sequences of variable regions of a heavy chain and a light chain of the targeted cynoglossus semilaevis IgM monoclonal antibody as shown in SEQ ID NO.1-12, the heavy chain subtype is IgG type, the light chain is kappa type, and the nucleotide and amino acid sequences of the variable regions of the heavy chain and the light chain of the targeted cynoglossus semilaevis IgM monoclonal antibody are subjected to codon optimization to obtain the cynoglossus semilaevis IgM monoclonal antibody. And constructing recombinant pATX1 vectors of heavy-chain and light-chain antibodies with a constant region sequence of a murine antibody respectively. The cynoglossus semilaevis IgM monoclonal antibody is cultured and expressed in vitro through co-transfection of XtenCHO cells, the chimeric immunoglobulin is good in reaction characteristic and high in titer (128000 or above), the chimeric immunoglobulin is easier to store compared with a hybridoma cell mode, and the risk of losing the specific antibody is avoided.

The invention discloses a time-resolved fluorescence immunochromatography test strip for detecting grouper IgM (Immunoglobulin M), which is characterized in that an anti-grouper IgM monoclonal antibody secreted by a hybridoma cell strain EpIgM-62 marked by time-resolved fluorescent microspheres is used as a detection marking antibody on a conjugate pad; an anti-grouper IgM monoclonal antibody secreted by a hybridoma cell strain EpIgM-41 is used as a detection line (T line) coated antibody, so that the time-resolved fluorescence immunochromatography test strip for detecting grouper IgM is constructed. The titers of the two monoclonal antibodies are 128000 or above, the test strip is good in specificity, high in sensitivity, strong in repeatability and convenient and fast to operate, the detection time is only 10 minutes, the detection sensitivity can reach 0.00625 mu g / mL, the IgM content in grouper serum can be accurately detected, and the test strip has important significance for evaluating the health condition and immune function of grouper.

The utility model discloses a fish Igm monoclonal antibody detection device, which comprises an enzyme linked immunosorbent assay analyzer, the enzyme linked immunosorbent assay analyzer comprises a detector main body, a detection bin and a placing rack, the detection bin is arranged in the middle of the front side of the detector main body, and the placing rack is slidably connected to the middle of the detection bin; the convenient assembly comprises four grooves, connecting shafts, torsional springs, first limiting plates and inclined plates, the grooves are formed in the two side edges of the containing frame, the connecting shafts are fixed to the inner sides of the grooves, the connecting shafts are sleeved with the lower ends of the first limiting plates, the two ends of the connecting shafts are sleeved with the torsional springs, one ends of the torsional springs are fixed to the side edges of the grooves, and the other ends of the torsional springs are fixed to the inclined plates. And the inclined plate is fixed at the upper end of the first limiting plate. The utility model relates to an elisa plate placing rack, aiming at solving the problems that when an elisa plate is placed, the elisa plate is held by two hands and the placing position is limited because the position of the placing rack moving out of a detection bin is relatively small, and the elisa plate can be placed at a correct position by a detector through multiple times of adjustment.

The invention discloses a high-stability chlamydia pneumoniae IgM antibody detection kit and a preparation method, and belongs to the technical field of in-vitro detection.The kit comprises a sample pad fixed to a PVC plate, a gold label pad, a reaction film coated with a detection line and a quality control line and a water absorption pad; the colloidal gold pad is loaded with a colloidal gold labeled mouse anti-human IgM monoclonal antibody, and the colloidal gold labeled mouse anti-human IgM monoclonal antibody is obtained by modifying the surfaces of colloidal gold particles with cysteamine, then labeling the antibody and carrying out targeted stabilization treatment through boric acid functionalized polyamide-amine dendritic macromolecules; composite functional microspheres are fixed on the sample pad, and the composite functional microspheres are polystyrene microspheres fixed with bilirubin oxidase and a polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer; according to the invention, through systematic optimization and innovative design, the detection performance, the stability and the anti-interference capability are remarkably improved.

The utility model discloses a portable fish Igm (Immunoglobulin m) monoclonal antibody colloidal gold test paper box which comprises a detection component, a detection component and a detection component, the drawing assembly comprises a mounting groove, a drawer, a foam block, a mounting hole, a first connecting belt, a first hook-and-loop fastener, a second hook-and-loop fastener and a fixing piece, the mounting groove is formed in the lower end of one face of the test paper box, the drawer is slidably mounted in the mounting groove, the foam block is placed in the drawer, and the mounting hole is formed in the top face of the foam block; the first connecting band is adhered to the lower side of one surface of the drawer, the first magic tape is fixedly connected to the lower end of the first connecting band, and the second magic tape is fixedly connected to the upper end of the first connecting band. The test paper box has the advantages that the drawer type design allows more reasonable utilization of the internal space of the test paper box, the storage positions and the number of the test tubes can be adjusted as required, and the test tubes are safely limited in the test paper box before and after sampling, so that the pollution of the external environment to samples can be reduced.

The application discloses an anti-Rofer IgM monoclonal antibody, a hybridoma cell strain and application thereof. The CDR sequences of a heavy chain variable region of the monoclonal antibody are shown as SEQ ID NO. 1-3, and the CDR sequences of a light chain variable region are shown as SEQ ID NO. 4-6. The monoclonal antibody can be obtained by secretion of the hybridoma cell strain with a preservation number of CCTCC NO: C2023153. The monoclonal antibody provided by the application can be used for specific detection of secreted IgM of the Rofer by Western blot and ELISA, and can be used for identification of IgM of the Rofer by flow cytometry + The B cell has the characteristics of good specificity, high sensitivity and strong affinity, and can be used for structural analysis of the IgM of the Rofer and detection of an immune response level, thereby laying a foundation for in-depth research on the immune system of the Rofer and establishment of an evaluation method for an immune effect of a vaccine of the Rofer with an antibody level as an index.

The application discloses a high-stability chlamydia pneumoniae IgM antibody detection kit and a preparation method, and belongs to the technical field of in-vitro detection. The kit comprises a sample pad, a gold mark pad, a reaction film coated with a detection line and a quality control line and a water absorption pad fixed on a PVC plate. The gold mark pad is loaded with colloidal gold labeled mouse anti-human IgM monoclonal antibody. The colloidal gold labeled mouse anti-human IgM monoclonal antibody is obtained by labeling the antibody after modifying the surface of colloidal gold particles with cysteamine and performing targeted stabilization treatment with boron-functionalized polyamide-amine dendrimers. The sample pad is fixed with composite functional microspheres. The composite functional microspheres are polystyrene microspheres fixed with bilirubin oxidase and polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer. The application has made significant progress in detection performance, stability and anti-interference ability through systematic optimization and innovative design.