Chlamydia pneumoniae IgM (immunoglobulin M) colloidal golden method kit and preparation method thereof

Abstract

The invention discloses a chlamydia pneumoniae IgM (immunoglobulin M) colloidal golden method kit which comprises recombinant chlamydia pneumoniae antigens enveloped on a nitrocellulose membrane detection line, goat anti-rat IgG (immunoglobulin G) antibodies enveloped on a quality control line and rat anti-human IgM monoclonal antibodies which have colloidal gold labels and are enveloped on a gold label pad, the concentration of the chlamydia pneumoniae antigens ranges from 1mg / ml to 2mg / ml and is measured by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), the concentration of the goat anti-rat IgG (immunoglobulin G) antibodies ranges from 1mg / ml to 3mg / ml, and the concentration of the rat anti-human IgM monoclonal antibodies ranges from 5g / mL to 30g / mL and is measured by the SDS-PAGE. The chlamydia pneumoniae IgM colloidal golden method kit has the advantages that the kid is speedy, simple, convenient and accurate and is high in sensitivity, and a judgment result can be read after integral operation time of dozens of minutes; and a colloidal gold quick detecting paper strip is made of the multi-epitope recombinant antigens and is simple and convenient to operate, low in cost, good in specificity and high in sensitivity, can be used for single-component detection and is popularized easily, and detection and control effects for chlamydia pneumoniae IgM are obvious.

Description

technical field

[0001] The invention relates to an antibody detection kit and a preparation method thereof, in particular to a chlamydia pneumoniae IgM antibody colloidal gold method detection kit and a preparation method thereof. Background technique

[0002] Chlamydia pneumoniae is an important pathogen of human respiratory diseases, which can cause acute and chronic respiratory diseases. Surveys show that 5-10% of community-acquired pneumonia, bronchitis and sinusitis are caused by Chlamydia pneumoniae. It often causes atypical pneumonia in adults and adolescents, and can also cause acute respiratory infections such as bronchitis, pharyngitis and tonsillitis. The clinical manifestations of Chlamydia pneumoniae respiratory infection are not typical, usually with sore throat and hoarseness onset, followed by coughing a few days to a week later, compared with other respiratory diseases, Chlamydia pneumoniae infection takes the longest time from onset to medical treatment, so...

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