46 results about "Gold labelling" patented technology

The invention discloses a gold label implant and positioning feature processing equipment thereof, and relates to the field of implantable medical instruments, a gold label is a cylinder, and the side wall of the gold label is provided with positioning features; positioning feature machining equipment comprises an electric spark machining device and a control piece. The control piece comprises a rotating assembly and a power connection assembly; the rotating assembly comprises a mounting frame, a motor arranged on the mounting frame, a first bevel gear connected with the motor, a second bevel gear meshed with the first bevel gear, a chuck piece fixed to the second bevel gear and a clamping piece arranged on the chuck piece. Positioning features are marked on the surface of the metal marker, friction force can be effectively increased, the implant is made of inert alloy materials and is easy to recognize and track, and conventional medical examination is not affected; the positioning feature machining equipment adopts electric spark machining, effectively fixes a gold-labeled raw material alloy thin wire, meanwhile, the gold-labeled raw material alloy thin wire is connected into an electrode, the rotating angle of the alloy wire can be controlled through a motor during machining, and positioning features are gradually punched on the side wall.

The invention relates to the technical field of gold label implantation, and discloses a gold label implant and an implantation device.The gold label implant comprises gold label bodies, convex patterns are arranged on the gold label bodies, connecting structures are arranged at the two ends of each gold label body, and the connecting structures are used for connecting every two adjacent gold label bodies; the connecting structure comprises an annular groove and an opening groove, the filling mechanism comprises a mounting block, a filling block is arranged on one side of the mounting block, a filling cavity is formed in the filling block, and a filling opening is formed in one side of the mounting block. The annular grooves and the mouth grooves are formed in the ends of the gold label bodies, so that every two adjacent gold label bodies can be connected through the protein lines, relative concentration of the positions of the multiple gold label bodies is facilitated, excessive dispersion is avoided, meanwhile, the protein lines used for connection can be dissolved, negative influences on the human body are avoided, and the service life of the gold label bodies is prolonged. The filling mechanism is arranged to fill the gold label bodies, and a plurality of gold label bodies can be filled at a time, so that the working efficiency is improved.

The invention discloses a real-time positioning method for a tumor target region and a gold label implant, and belongs to the technical field of tumor localization, and the method specifically comprises the following steps: implanting a gold label in a tumor region, constructing a gold label-tumor dynamic model through CT, and obtaining a respiratory phase feature vector; realizing space matching of the two-dimensional projection and the three-dimensional model by using an improved hybrid registration algorithm, and obtaining a tumor compound displacement vector; establishing a respiration-displacement correlation model through time sequence alignment, generating a tumor movement map containing a real-time position and a predicted trajectory, and updating the reference coordinate system; establishing a rigid safety boundary based on a gold label, analyzing soft tissue texture and a prediction trajectory in combination with a convolutional neural network to generate an elastic treatment boundary, and dynamically adjusting a dose weight of a non-label area; and finally, submillimeter-level dynamic tracking is executed through a multi-leaf collimator. The problems of tumor motion uncertainty and individual deformation difference are solved, and the target area coverage precision and the dose distribution reasonability are improved.

The invention relates to an implantation device for gold labels or radioactive particles, belongs to the technical field of medical instruments, and solves one of the problems that the number of the gold labels or radioactive particles loaded at one time is insufficient and the implantation counting accuracy of the gold labels or radioactive particles is poor in an existing implantation device. The device comprises a puncture outfit, a loader and an implantation needle, and the puncture outfit is used for puncturing into the body of a patient and puncturing to a tumor area; the loader can load a plurality of gold labels or radioactive particles and can be clamped on the puncture outfit; and the implantation needle is used for implanting the gold label or the radioactive particles loaded in the loader into a tumor area. A plurality of gold labels or radioactive particles can be loaded at a time through the loader, one-time filling and multi-batch continuous implantation are achieved, the device does not need to be frequently disassembled to supplement the gold labels or radioactive particles, the interruption frequency of clinical operation is greatly reduced, the operation efficiency and operation continuity are improved, and the large-dose implantation requirement is met.

This invention provides a colloidal gold labeling method with improved sensitivity, its applications, and products, relating to the field of immunochromatography technology. The colloidal gold labeling method provided by this invention involves mixing a protein to be labeled, treated with a protein activator, with a colloidal gold solution for labeling reaction to obtain a gold-labeled protein. The protein activator includes a nonionic surfactant, a glycoprotectant, and a reducing agent. Through the synergistic effect of these components, the interactions between the gold-labeled conjugates are effectively reduced, preventing aggregation and improving the stability of the gold-labeled conjugates while protecting protein activity. The pH of the colloidal gold solution is 0.3-0.8 lower than the isoelectric point of the protein to be labeled. Under these conditions, the gold-labeled conjugates formed have good stability and high labeling efficiency. This method is simple to operate, the prepared gold-labeled conjugates have high labeling efficiency and good stability, and the prepared reagent kit has high sensitivity and is less prone to missed detections.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

1. The name of the design product: packing box (high ditch gold mark). 2. The use of the design product: packing box for wine. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

1. Name of the designed product: food packaging box (gold nut). 2. Use of the designed product: packaging food. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo that best shows the design points: perspective view.

The invention relates to the technical field of gold-labeled implants, and discloses a pure gold-labeled implant with a positioning characteristic, the pure gold-labeled implant comprises at least two markers, and the markers are injected through a gold-labeled injector; the connecting line is a collagen chain and a fixation stripe group and is arranged on the marker, and after the marker is located in the target tissue, the fixation stripe group is extruded by the target tissue, so that the marker can be fixed in the target tissue; the fixing grain group comprises a fixing piece arranged on the marker; wherein the left view of the marker is of a circular structure, the top view of the marker is divided into four areas by two mutually perpendicular axial cross sections in the marker, the fixing pieces are located in the areas, and through a limiting groove, a connecting line, a mushroom-shaped third fixing piece and a multi-layer groove structure which are arranged in the pure gold label, the fixing pieces are fixed to the left side of the marker. Therefore, the human tissue can extend into the gold label implant to be matched with the barb to fix the gold label implant, and displacement of the implant is avoided.

The invention provides a specific nucleic acid aptamer of alpha-camarine and a test paper detection method of the specific nucleic acid aptamer. The nucleic acid aptamer has a nucleic acid sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The invention also provides a lateral flow chromatography detection test strip for alpha-calasodine. The lateral flow chromatography detection test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the conjugate pad is sprayed with a gold-labeled probe containing the aptamer and gold nanoparticles; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is fixedly provided with a first capture molecule, the first capture molecule is streptavidin, the quality control line is fixedly provided with a second capture molecule, and the second capture molecule is a conjugate of a streptavidin-biotinylated aptamer. According to the nucleic acid aptamer, the gold-labeled probe and the test strip disclosed by the invention, the detection efficiency of the alpha-camarin detection method is greatly improved, and the on-site instant demand is met.

The invention discloses a method for detecting lactoferrin in milk based on a competition method test strip, which comprises the following steps: coupling heparin molecules with bovine serum albumin to obtain a heparin molecule-bovine serum albumin artificial antigen; modifying gold nanoparticles by using an artificial antigen to form a gold label I, and constructing a competition method test strip by matching with a gold label II modified by mouse IgG (Immunoglobulin G); a milk sample is subjected to centrifugal fat removal, pH adjustment and casein precipitation pretreatment and then dropwise added to the test strip, and the LF concentration is calculated through a T line gray value and a working curve. Detection can be completed within about 10-15 min, specificity is high (interference of impure protein is avoided), on-site rapid quantitative / semi-quantitative detection of LF in milk can be achieved, the antiviral activity of LF can be indirectly reflected through the binding strength of heparin and LF, the method is suitable for production quality inspection, dealer sampling inspection and consumer self-inspection, and the detection sensitivity reaches 1 microgram / mL.

The invention belongs to the technical field of biological material structure and performance analysis, and discloses a rapid qualitative and quantitative detection kit for a collagen triple-helix structure and a detection method. The detection kit comprises an enzymolysis system solution A of a non-triple helix structure of specific enzymolysis collagen; an ultrafiltration centrifugal tube; the enzymolysis system liquid B is used for performing non-specific enzymolysis on small-molecule polypeptide; colloidal gold test paper; the colloidal gold test paper is sequentially provided with a sample pad, a combination pad, an NC membrane and a water absorption pad from left to right; the NC membrane is sequentially coated with a T line and a C line from left to right; the marker on the combination pad is a gold-labeled antibody prepared from a hydroxyproline monoclonal antibody and colloidal gold particles through electrostatic coupling; and a coating substance on the T line is a hydroxyproline-carrier protein conjugate. The detection kit provided by the invention realizes a rapid, simple and good-specificity qualitative and quantitative detection method for the collagen triple-helix structure.

The invention relates to the field of tumor treatment instruments, and discloses a miniature gold label implant and forming equipment thereof, an implant body is cylindrical, and a plurality of wing plates are arranged on the side wall of the implant body; the equipment comprises a forming assembly and a feeding assembly, wherein the forming assembly comprises a base, a limiting disc arranged on the base, a limiting groove formed in the limiting disc and a cutter arranged in the limiting groove; the miniature gold label implant can be placed in tumor tissue and then identified and positioned to the marker body by a radiotherapy instrument, so that the radiotherapy instrument can accurately position the position of the tumor, the wing plates are designed into barbs, the implant can be prevented from shifting, the two ends of the gold label are designed into a blunt round shape and a sharp shape, and the stability is enhanced by utilizing tissue wrapping difference; the coating is arranged on the side wall of the bottom of the wing plate, the wing plate expands when encountering tissue fluid in the tissue, and the tumor tissue is physically clamped; meanwhile, forming equipment is provided, the side wall of the gold-labeled raw material alloy wire harness can be machined, and a wing plate structure is cut out.

The application discloses a kind of for HBV PreS1 antibody qualitative detection test strip and its preparation method and application, it is related to virology, immunology, biological detection technology and immunochromatography analysis technical field.The test strip is based on the specific recognition principle of antigen and antibody, the specific binding of PreS1 antigen and PreS1 antibody is realized, the rapid detection of PreS1 antibody in serum or plasma sample, it is sequentially assembled by sample pad, gold mark pad, nitrocellulose membrane (NC membrane) and water absorption pad, wherein, gold mark pad is fixed with colloidal gold labeled anti-human IgG antibody as gold mark probe, NC membrane detection line (T line) is coated HBV PreS1 antigen, for capturing PreS1 antibody in sample, NC membrane quality control line (C line) is coated HB2 antibody, for judging whether the test strip detection process is effective.The test strip in the application does not need complex instrument equipment, detection process is simple, fast and fast, and has good sensitivity and specificity, can be used for hepatitis B vaccine immune effect evaluation and large-scale screening.

The invention is applicable to the technical field of animal virus detection, and provides a PVC2 and PRRSV (porcine reproductive and respiratory syndrome virus) double colloidal gold test strip and application thereof. Ferritin nanocage fusion protein of PCV2 and PRRSV is taken as a capture antibody; a gold-labeled PCV2 monoclonal antibody and a gold-labeled PRRSV monoclonal antibody are used as detection antibodies, and the gold-labeled PCV2 monoclonal antibody and the gold-labeled PRRSV monoclonal antibody are obtained by labeling a specific monoclonal antibody composition by using a gold labeling technology and treating the specific monoclonal antibody composition. The prepared double colloidal gold test strip is high in specificity, low in production cost, easy to operate and visual in result, PCV2 and PRRSV infection can be rapidly identified and diagnosed on site at the same time, and the double colloidal gold test strip has wide market application prospects.

1. The name of the design product: packing bottle (red dog gold mark series). 2. The use of the design product: the design product is used for container. 3. The design points of the design product: in shape. 4. The picture or photo that can best show the design points: perspective view 1.

1.The name of the design product: packaging jar (6.05 kg special gold label soy sauce). 2.The use of the design product: the design is used for food packaging. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

This application provides a method and system for recommending gold marker implantation sites, relating to the field of image-guided radiotherapy technology. The method includes acquiring a patient's CT image data; determining several gold marker implantation points based on the CT image data; combining the gold marker implantation points according to preset aggregation rules to construct several gold marker point sets; evaluating the three-dimensional geometric stability index and the two-dimensional observable stability index of each gold marker point set; combining the preset evaluation rules and the geometric stability index and observable stability index corresponding to each gold marker point set to select the optimal gold marker point set from all gold marker point sets; using the gold marker implantation points in the optimal gold marker point set as recommended gold marker implantation sites; and proactively recommending the optimal implantation point by quantitatively evaluating the geometric stability and observable stability of the gold marker based on CT images before implantation, thereby preventing distribution degradation problems, avoiding rework after implantation, and ensuring tracking accuracy and treatment efficiency from the source.

The invention discloses colloidal gold chromatography test paper and a kit for detecting platelet CD36 antigen, the colloidal gold chromatography test paper comprises a bottom plate, a sample pad, a gold-labeled combination pad, a nitrocellulose membrane and a water absorption pad, the gold-labeled combination pad is coated with a colloidal gold labeled mouse anti-human CD36 IgG2a monoclonal antibody and a colloidal gold labeled quality control rabbit IgG polyclonal antibody; the nitrocellulose membrane is provided with a detection line coated with a human platelet CD36 antigen capture antibody and a quality control line coated with quality control goat anti-rabbit polyclonal antibody molecules. When the kit is used for detecting the CD36 antigen, a detected sample is fully split by the platelet lysis buffer and then is added into the sample adding hole of the kit, and a detection result can be observed after waiting for 10-15 minutes. By adopting the detection mode provided by the invention, the CD36 antigen on the human platelets can be quickly, simply, conveniently, sensitively and specifically detected.

The application discloses an aptamer-nano gold test strip for screening human pulmonary tuberculosis and an application thereof, and belongs to the technical field of biological detection. The application successfully constructs an aptamer-nano gold test strip for screening human pulmonary tuberculosis, realizes rapid detection of a pulmonary tuberculosis marker ESAT-6 antigen, adopts mercapto-modified aptamer to modify nano gold to form stable gold label probes, adopts a mixed solution composed of a biotin-modified aptamer solution and a streptavidin solution, and constructs an ESAT-6 antigen test strip by using an ESAT-6 antigen solution. The constructed test strip has good sensitivity in ESAT-6 antigen detection, has good detection effect in the antigen concentration range of 100 pg / mL-500 pg / mL, and has good specificity, stability, repeatability and accuracy.

The present application relates to the fields of biotechnology and food detection technology, and discloses a rice flower honey colloidal gold immunochromatographic test strip as well as a preparation method and application thereof. Through proteomic analysis, the Ycf1 protein of chloroplast origin is identified as a specific marker of the plant source in rice flower honey, and a rice flower honey colloidal gold immunochromatographic test strip is developed. The test strip comprises a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are connected in sequence. The gold label pad is coated with gold-labeled antibodies formed by the combination of a monoclonal antibody against a specific segment of the Ycf1 protein and colloidal gold. The nitrocellulose membrane is provided with a detection line and a quality control line. The detection line is coated with a monoclonal antibody against the Ycf1 protein, and the quality control line is coated with a goat anti-mouse IgG. The test strip is simple to operate, and the detection can be completed within 15 minutes. The test strip has high sensitivity, strong specificity, and the results are intuitive and reliable, and is suitable for the on-site rapid screening and identification of the authenticity of rice flower honey.

This invention discloses a qualitative test strip for HBV PreS1 antibody, its preparation method, and its applications, relating to the fields of virology, immunology, biodetection technology, and immunochromatographic analysis. Based on the principle of antigen-antibody specific recognition, the test strip achieves rapid detection of PreS1 antibodies in serum or plasma samples through the specific binding of PreS1 antigen and PreS1 antibody. It is assembled sequentially from a sample pad, a gold-labeled pad, a nitrocellulose membrane (NC membrane), and an absorbent pad. The gold-labeled pad is immobilized with colloidal gold-labeled anti-human IgG antibody as a gold probe. The NC membrane detection line (T line) is coated with HBV PreS1 antigen to capture PreS1 antibodies in the sample, and the NC membrane control line (C line) is coated with HB2 antibody to determine the effectiveness of the test strip's detection process. The test strip of this invention requires no complex instruments or equipment, the detection process is simple, rapid, and has good sensitivity and specificity, making it suitable for evaluating the immunization efficacy of hepatitis B vaccines and for large-scale screening.

The present invention belongs to the technical field of molecular detection. Provided are a gold-labeled chain length-mediated gold colloid test strip sensor, and a preparation method therefor and the use thereof. According to the provided gold-labeled chain length-mediated gold colloid test strip sensor, two gold-labeled probes are designed to be completely complementary to a wild-type site (T / T) and a mutant site (C / C), respectively. The chain length of the gold-labeled probes and a sodium chloride concentration are optimized, and two test strips are constructed on the basis of the two gold-labeled probes, respectively. In actual operation, a sample only needs to be loaded onto the two test strips separately to effectively distinguish SNP sites while eliminating the occurrence of false positive results. Moreover, the gold-labeled chain length-mediated gold colloid test strip sensor has a high accuracy, and has the advantages of rapidness, visualization, and low costs.

The invention discloses a multi-immunochromatography test strip with unified detection limit for detecting four aminoglycoside drugs and application of the multi-immunochromatography test strip. The test strip comprises a water absorption plate, a detection pad, a colloidal gold pad, a sample pad and a PVC (polyvinyl chloride) bottom plate, wherein a gold-labeled antibody is fixed on the surface of the colloidal gold pad, and when a sample flows through the colloidal gold pad, the gold-labeled antibody is dissolved and serves as a tracer molecule to migrate along with a liquid phase; four detection areas T1, T2, T3 and T4 and a quality control line area C are arranged on the detection pad, a kanamycin antigen, a gentamicin antigen, a neomycin antigen and a spectinomycin antigen are sprayed on the four detection areas respectively, and a goat anti-mouse antibody is sprayed on the quality control line area C. According to the present invention, the expensive reagent and the base material are not required, the production cost is low, the operation is simple, the professional training is not required, the detection efficiency is high, the detection cost is low, the multiple immunochromatography test strip is used for simultaneously detecting kanamycin, gentamicin, neomycin and spectinomycin, and the detection limits can achieve 5 ng / mL.

The invention discloses a preparation method and application of a colloidal gold test strip for detecting a group A rotavirus antibody. The gold-labeled protein used by the test strip is prokaryotically expressed and soluble RVA-VP6-3P recombinant protein, and the protein has rich group-specific epitopes, so that the specificity and sensitivity of the gold-labeled protein are ensured, the economic cost for obtaining and purifying the target protein is reduced, and a foundation is laid for establishment, popularization and application of an antibody detection method. The RVA-VP6-3P is labeled with colloidal gold, the detection line is coated with SPA, the quality control line is coated with a monoclonal antibody 1M1F9 capable of specifically recognizing the RVA-VP6-3P, and then the test strip is assembled. The test strip has no cross reactivity with positive serum antibodies of other common swine disease viruses, has the characteristics of convenience in operation, less time consumption, high sensitivity, strong specificity and the like, can complete the detection of the group A rotavirus antibody in 5-15 minutes, and is convenient for immediate use in a clinical first line.

The invention belongs to the technical field of detection, and particularly relates to a fluorescent biosensor for lead ion detection and a preparation method and application thereof. The preparation method comprises the following steps: carrying out sulfydryl activation treatment on sulfo-GR-5S and sulfo-Poly-T by using TCEP, then taking sulfo-GR-5S and sulfo-Poly-T and gold nanoparticles as reactants, and constructing a primary gold-labeled probe through a halide ion auxiliary modification strategy; taking the primary gold-labeled probe and biotin-labeled GR-5E as reactants, and performing hybridization annealing treatment to obtain a bifunctional gold-labeled probe; and by taking the bifunctional gold-labeled probe and streptavidin magnetic beads as reactants, carrying out biotin-streptavidin binding reaction, so as to obtain the AuNP-coated MB probe. The probe is used for lead ion detection, the linear detection range is 1-500 nM, the detection limit is 0.34 nM, the probe shows excellent performance in actual sample analysis, and the recovery rate reaches 91.6-117%.

The invention relates to the technical field of clenbuterol detection, and particularly discloses beef clenbuterol colloidal gold detection equipment and a beef clenbuterol colloidal gold detection method. The detection unit comprises a base station, and a test strip is arranged on the base station; and the sealing filling unit comprises a sealing cover, a sealing cavity is formed in the sealing cover, a filling bin is arranged in the sealing cavity, and an oscillating piece is arranged in the filling bin. The sealing cover and the base form a closed sealing cavity, and the test strip is isolated from the outside, so that the influence of impurities in air, cross contamination or human interference on a detection result is effectively avoided, and the test strip is particularly suitable for accurate analysis of a trace sample; the oscillating part can perform active oscillation treatment on a sample solution and a buffer solution before filling, so that the sample solution and the buffer solution are uniformly mixed before being filled into a test strip, the subsequent gold-labeled antibody is effectively promoted to be fully dissolved, a CLB antigen and the gold-labeled antibody are promoted to have a more thorough competitive reaction, and the false negative or quantitative deviation risk caused by non-uniform mixing is reduced.

The application provides a composition type dissociation solution for whole blood 25OHD immunochromatographic detection, a preparation method and application thereof, and relates to the technical field of in vitro diagnostic reagents. The dissociation solution is mixed by a dissociation trigger solution containing a non-micellar type zwitterionic additive and a dissociation salt, a blood cell protection-flow control solution containing a membrane protection block copolymer, and an exchange solubilization-immune compatibility solution containing an exchange type hydrophobic guest solubilizer, and efficient dissociation of 25OHD and vitamin D binding protein is realized in a near neutral / weak acid mild environment. The problems of destroying the stability of gold-labeled antibody and colloidal gold, causing hemolysis and membrane blockage, and requiring additional incubation and centrifugation are solved, and the advantages of inhibiting hemolysis, smooth flow and strong anti-interference ability are combined. Without pretreatment, it can be adapted to instant detection scene. It can be applied to the preparation of whole blood 25OHD immunochromatographic test strips, the detection result is accurate and stable, and the industrialization adaptability is strong.

This invention belongs to the field of pathogen and toxin detection technology, specifically relating to a polyclonal antibody colloidal gold test strip for detecting Clostridium perfringens α toxin and its application. The polyclonal antibody colloidal gold test strip includes a PVC base plate, on which a sample pad, a gold-labeled pad, a chromatography membrane, and an absorbent pad are sequentially overlapped. The gold-labeled pad is coated with colloidal gold particles labeled with polyclonal antibodies against Clostridium perfringens α toxin. A detection line and a control line are sequentially arranged on the chromatography membrane. The detection line is coated with polyclonal antibodies against Clostridium perfringens α toxin, and the control line is coated with goat anti-rabbit IgG (H+L) antibody. The amino acid sequence of the recombinant protein of Clostridium perfringens α toxin is shown in SEQ ID NO.3. The polyclonal antibody colloidal gold test strip provided by this invention can be used for the detection of Clostridium perfringens α toxin, with a detection limit of 31.25 μg / mL.

The invention belongs to the technical field of detection and analysis, and particularly relates to a colloidal gold test strip and kit for quantitatively detecting perfluorooctanoic acid in human serum and a use method of the colloidal gold test strip and kit. The colloidal gold test strip and the kit based on the special monoclonal antibody are innovatively researched and developed, the monoclonal antibody has a unique gene sequence, and the detection sensitivity of perfluorooctanoic acid in human serum is remarkably improved. When the test strip is used, a sample to be detected only needs to be dropwise added on the sample pad of the test strip, the sample moves towards the water absorption pad under the capillary action, and the sample interacts with a colloidal gold marker when flowing through the colloidal gold pad. Compared with traditional instrument detection, the colloidal gold immunochromatography technology does not need professional operation and complex equipment, the detection process is simple, convenient and rapid, high sensitivity is kept, the content detection of the perfluorooctanoic acid in human serum can be rapidly completed on site, and the detection sensitivity is high. And an efficient and practical new scheme is provided for emergency public health event response and pollutant health risk management.