99 results about "Gold labelling" patented technology

The utility model belongs to the field of medical equipment and relates to a radiotherapy positioning gold label loader which comprises a handheld base, a loading device and a positioning device are arranged on the upper portion of the handheld base, and a guide nozzle is arranged on the lower portion of the handheld base. The loading device comprises a loading body and a cover body, the cross section of the loading body is in a regular hexagon shape, a rotating shaft is arranged on the handheld base, and the loading body is installed on the rotating shaft; a containing cavity is formed in the loading body and penetrates through the height direction of the loading body; the cover body is arranged on the upper side of the loading body and fixedly connected with the rotating shaft, and a through hole is formed in the cover body and right faces the guide nozzle; the positioning device is arranged on one side of the loading device, a blind hole perpendicular to the side face of the loading body is formed in the positioning device, a fixing column is arranged in the blind hole, and a spring is arranged between the fixing column and the blind hole; and when the positioning device is relatively fixed with a certain side surface of the loading body, the accommodating cavity is arranged opposite to the through hole and the guide nozzle. The device can be matched with implanted gold labels and is simple in structure and convenient and smooth to operate.

The invention relates to a method for measuring the fluorine distribution rate in a mineral raw material by using a BPMA system, and the method comprises the following steps: pretreating a sample to be measured to obtain a polished section sample of which the surface is plated with a carbon conductive film; placing the X-ray plate sample and the gold standard sample in a scanning electron microscope of a BPMA system, and determining test conditions by adopting a back scattering electron imaging mode; carrying out automatic measurement by adopting a BPMA system, and determining the composition and the mass percentage content ratio of minerals in the sample and the mass percentage content of fluorine in each mineral; and calculating the grade of fluorine in the sample and the distribution rate of fluorine in the mineral. The method provided by the invention has the advantages of simple sample preparation, modularized test process, convenient and reliable data acquisition and the like, and can be widely applied to research on the distribution rate of valuable elements in mineral raw materials.

The utility model relates to a one-piece gold label and a gold label implanting device, belongs to the technical field of medical instruments, and solves the problems that the distance between two gold label bodies is uncontrollable and the gold label implanting quality is influenced in the prior art. The gold label comprises a first gold label body, a second gold label body and a supporting body, the two ends of the supporting body are fixedly connected with the first gold label body and the second gold label body respectively, and the supporting body is used for keeping the distance between the first gold label body and the second gold label body unchanged when the first gold label body and the second gold label body are implanted into the body of a patient. According to the utility model, the first gold label body and the second gold label body are fixedly connected through the support body, and the distance between the first gold label body and the second gold label body is fixed, so that the implantation quality of the gold label is improved, the immediate position of a tumor is accurately marked, and the treatment effect is improved.

The invention discloses a preparation method and application of a colloidal gold immunochromatography test strip for detecting IDH1 mutant protein, and belongs to the field of rapid immunodetection. The colloidal gold chromatography test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane, a water absorption pad and colloidal gold, the nitrocellulose membrane is sequentially provided with a detection line T and a quality control line C in the flowing direction of a sample, the detection line T is coated with IDH1 mutant protein, and the quality control line C is coated with a second antibody corresponding to a monoclonal antibody of the IDH1 mutant protein. Clinical tumor tissue samples collected in real time in glioma resection surgery are treated, an object to be detected and an immune gold label are mixed, incubated and dropped in a test strip sample adding hole, and the object to be detected runs along the test strip under the driving of a buffer solution and generates visual color change. The test strip has the advantages of being rapid in reaction, easy and convenient to operate, convenient to carry, high in specificity and the like, makes up the blank of an IDH1 mutant protein concentration quantitative method, and has good clinical application value.

The invention belongs to the technical field of medicine detection, and particularly relates to a method for reducing a HOOK effect in colloidal gold immunochromatography detection and application. The upstream of the T line is provided with the B line, the B line is coated with the first antibody which is not marked, the combination pad is coated with the first antibody which is marked by colloidal gold, and the first antibody which is marked by colloidal gold and the first antibody which is not marked can be specifically combined with an analyte antigen in a sample to be detected; in addition, the T line is coated with a second antibody, the second antibody and a first antibody marked by colloidal gold, and the unmarked first antibody is a pair of antibodies capable of performing specific immunoreaction with different epitopes of the same antigen. According to the invention, due to the arrangement of the B line, the competitive strength of a large number of free analyte antigens and gold-labeled antibody-analyte antigen compounds on the binding site of the T line is favorably reduced, so that the HOOK effect is reduced, and the detection accuracy is remarkably improved.

The utility model discloses a gold label pad metal spraying and drying device, which relates to the technical field of metal spraying and drying devices and comprises a metal spraying and drying frame, a collecting tank is arranged at the upper end of the metal spraying and drying frame, a metal spraying and drying machine shell is fixedly mounted at the upper end of the metal spraying and drying frame, and a metal spraying mechanism is arranged at the upper end of the metal spraying and drying machine shell. Side doors are symmetrically arranged on the outer side of the metal spraying and drying machine shell, pushing air cylinders are symmetrically and fixedly installed at the upper end of the metal spraying and drying machine shell, and the output ends of the pushing air cylinders movably stretch into the inner side of the metal spraying and drying machine shell and are fixedly provided with assembling blocks. By the adoption of the structure, the electric drying plate is inserted into the connecting groove of the heat insulation plate through the connecting plate, the connecting block on the connecting plate and the connecting groove are fastened through the mounting bolt after the electric drying plate is inserted, and on the contrary, when the electric drying plate is disassembled, only the mounting bolt needs to be taken down, and then the electric drying plate can be disassembled. And the electric drying plate is pushed out from the connecting groove to the groove opening direction, so that the electric drying plate can be conveniently and quickly disassembled, assembled and maintained.

The invention discloses a gold-labeled antibody purification device which comprises a centrifugal machine and a motor, the centrifugal machine is provided with a control program and an operation screen, the motor is fixedly connected with a fixing ring, a rotating shaft of the motor is rotatably connected with a quick-change assembly, the fixing ring is rotatably connected with the quick-change assembly, the quick-change assembly is detachably connected with a supporting frame, and the supporting frame is detachably connected with the centrifugal machine. The support frame is hinged with at least one group of test tube clamping components, the test tube clamping components can rotate by a certain angle relative to the support frame and are locked, the clamping components are provided with air bags, the air bags are used for clamping test tubes, the clamping components are connected with a fixing ring through an air path, the fixing ring is connected to an air pump through an air pipe, and the air pump is connected with the air bag. The fixing ring is further provided with a pressure sensing unit, the pressure sensing unit can make the air pump stop working, the clamping assembly can be rapidly replaced, the angle of the test tube can be adjusted, clamping is stable, the adaptability of the device is wider, and the centrifugal quality during gold-labeled antibody purification can be improved.

The invention discloses a gold label implant and positioning feature processing equipment thereof, and relates to the field of implantable medical instruments, a gold label is a cylinder, and the side wall of the gold label is provided with positioning features; positioning feature machining equipment comprises an electric spark machining device and a control piece. The control piece comprises a rotating assembly and a power connection assembly; the rotating assembly comprises a mounting frame, a motor arranged on the mounting frame, a first bevel gear connected with the motor, a second bevel gear meshed with the first bevel gear, a chuck piece fixed to the second bevel gear and a clamping piece arranged on the chuck piece. Positioning features are marked on the surface of the metal marker, friction force can be effectively increased, the implant is made of inert alloy materials and is easy to recognize and track, and conventional medical examination is not affected; the positioning feature machining equipment adopts electric spark machining, effectively fixes a gold-labeled raw material alloy thin wire, meanwhile, the gold-labeled raw material alloy thin wire is connected into an electrode, the rotating angle of the alloy wire can be controlled through a motor during machining, and positioning features are gradually punched on the side wall.

1. Name of the designed product: Spatula (gold mark wood). 2. Use of the designed product: For kitchen utensils. 3. Design points of the designed product: In shape. 4. Picture or photo that best shows the design points: Perspective view.

The invention relates to the technical field of gold label implantation, and discloses a gold label implant and an implantation device.The gold label implant comprises gold label bodies, convex patterns are arranged on the gold label bodies, connecting structures are arranged at the two ends of each gold label body, and the connecting structures are used for connecting every two adjacent gold label bodies; the connecting structure comprises an annular groove and an opening groove, the filling mechanism comprises a mounting block, a filling block is arranged on one side of the mounting block, a filling cavity is formed in the filling block, and a filling opening is formed in one side of the mounting block. The annular grooves and the mouth grooves are formed in the ends of the gold label bodies, so that every two adjacent gold label bodies can be connected through the protein lines, relative concentration of the positions of the multiple gold label bodies is facilitated, excessive dispersion is avoided, meanwhile, the protein lines used for connection can be dissolved, negative influences on the human body are avoided, and the service life of the gold label bodies is prolonged. The filling mechanism is arranged to fill the gold label bodies, and a plurality of gold label bodies can be filled at a time, so that the working efficiency is improved.

The invention discloses a real-time positioning method for a tumor target region and a gold label implant, and belongs to the technical field of tumor localization, and the method specifically comprises the following steps: implanting a gold label in a tumor region, constructing a gold label-tumor dynamic model through CT, and obtaining a respiratory phase feature vector; realizing space matching of the two-dimensional projection and the three-dimensional model by using an improved hybrid registration algorithm, and obtaining a tumor compound displacement vector; establishing a respiration-displacement correlation model through time sequence alignment, generating a tumor movement map containing a real-time position and a predicted trajectory, and updating the reference coordinate system; establishing a rigid safety boundary based on a gold label, analyzing soft tissue texture and a prediction trajectory in combination with a convolutional neural network to generate an elastic treatment boundary, and dynamically adjusting a dose weight of a non-label area; and finally, submillimeter-level dynamic tracking is executed through a multi-leaf collimator. The problems of tumor motion uncertainty and individual deformation difference are solved, and the target area coverage precision and the dose distribution reasonability are improved.

The invention relates to an implantation device for gold labels or radioactive particles, belongs to the technical field of medical instruments, and solves one of the problems that the number of the gold labels or radioactive particles loaded at one time is insufficient and the implantation counting accuracy of the gold labels or radioactive particles is poor in an existing implantation device. The device comprises a puncture outfit, a loader and an implantation needle, and the puncture outfit is used for puncturing into the body of a patient and puncturing to a tumor area; the loader can load a plurality of gold labels or radioactive particles and can be clamped on the puncture outfit; and the implantation needle is used for implanting the gold label or the radioactive particles loaded in the loader into a tumor area. A plurality of gold labels or radioactive particles can be loaded at a time through the loader, one-time filling and multi-batch continuous implantation are achieved, the device does not need to be frequently disassembled to supplement the gold labels or radioactive particles, the interruption frequency of clinical operation is greatly reduced, the operation efficiency and operation continuity are improved, and the large-dose implantation requirement is met.

This invention provides a colloidal gold labeling method with improved sensitivity, its applications, and products, relating to the field of immunochromatography technology. The colloidal gold labeling method provided by this invention involves mixing a protein to be labeled, treated with a protein activator, with a colloidal gold solution for labeling reaction to obtain a gold-labeled protein. The protein activator includes a nonionic surfactant, a glycoprotectant, and a reducing agent. Through the synergistic effect of these components, the interactions between the gold-labeled conjugates are effectively reduced, preventing aggregation and improving the stability of the gold-labeled conjugates while protecting protein activity. The pH of the colloidal gold solution is 0.3-0.8 lower than the isoelectric point of the protein to be labeled. Under these conditions, the gold-labeled conjugates formed have good stability and high labeling efficiency. This method is simple to operate, the prepared gold-labeled conjugates have high labeling efficiency and good stability, and the prepared reagent kit has high sensitivity and is less prone to missed detections.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

1. The name of the design product: packing box (high ditch gold mark). 2. The use of the design product: packing box for wine. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

1. Name of the designed product: food packaging box (gold nut). 2. Use of the designed product: packaging food. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo that best shows the design points: perspective view.

The application discloses a microbial nano-enzyme probe, a method for detecting salbutamol and application, and comprises a salbutamol monoclonal antibody and a signal carrier, wherein the signal carrier is a yeast cell loaded with iridium oxide nanoparticles. The application firstly constructs a probe by using a yeast cell loaded with iridium oxide nanoparticles in the detection of an immunochromatographic test strip, uses [3, 3', 5, 5' tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2)] as a substrate to trigger a color developing reaction, can realize high-sensitivity target detection, and the detection limit is as low as 0.012 ng / mL, which is 55 times higher than the sensitivity of a traditional gold-labeled test strip. In addition, the application has a good application prospect, can provide a universal platform for sensitive, accurate and rapid detection of food-borne stimulants, and can be successfully applied to the detection of salbutamol in pig liver and beef samples.

The invention relates to a mouse anti-PBP2a hybridoma cell line, a monoclonal antibody and an application thereof. The PBP2a protein of Staphylococcus aureus is extracted as an antigen. After obtaining a high-purity antigen, a good immune response is evoked in mice. Then, hybridoma technology is used to screen two cell lines with high affinity and specificity and prepare corresponding antibodies. The two antibodies are used as a gold-labeled antibody and a coating antibody in a colloidal gold immunoassay kit. The kit can be used to detect the PBP2a protein in methicillin-resistant Staphylococcus aureus and can be used for the development of related in vitro diagnostic reagents.

The present invention discloses an automatic sampling gold label penetration detection device, which belongs to the field of gold label penetration detection. The device comprises an installation box, wherein two arc-shaped pressure blocks are symmetrically hinged on both sides of the box opening of the installation box, and two pressure strips are symmetrically slidably connected on both sides of the box opening of the installation box. When the two arc-shaped pressure blocks approach each other, the two pressure strips synchronously squeeze the fingertips; a needle is linearly slidably connected to the middle part of the installation box, and the needle faces the fingertips. After the two pressure strips synchronously squeeze the fingertips, the needle moves back and forth vertically for a stroke to pierce the fingertips for sampling; the device places the fingertips of the fingers between the two arc-shaped pressure blocks so that the fingertips are between the two pressure strips, controls the two arc-shaped pressure blocks to approach each other, fixes the nail part of the finger, and at the same time pushes the fingertips to approach between the two pressure strips. At the same time, the two pressure strips approach each other to squeeze the fingertips, and then the needle moves toward the fingertips to pierce the fingertips, thereby completing the sampling operation of fingertip blood.

The invention relates to the technical field of gold-labeled implants, and discloses a pure gold-labeled implant with a positioning characteristic, the pure gold-labeled implant comprises at least two markers, and the markers are injected through a gold-labeled injector; the connecting line is a collagen chain and a fixation stripe group and is arranged on the marker, and after the marker is located in the target tissue, the fixation stripe group is extruded by the target tissue, so that the marker can be fixed in the target tissue; the fixing grain group comprises a fixing piece arranged on the marker; wherein the left view of the marker is of a circular structure, the top view of the marker is divided into four areas by two mutually perpendicular axial cross sections in the marker, the fixing pieces are located in the areas, and through a limiting groove, a connecting line, a mushroom-shaped third fixing piece and a multi-layer groove structure which are arranged in the pure gold label, the fixing pieces are fixed to the left side of the marker. Therefore, the human tissue can extend into the gold label implant to be matched with the barb to fix the gold label implant, and displacement of the implant is avoided.

The invention provides a specific nucleic acid aptamer of alpha-camarine and a test paper detection method of the specific nucleic acid aptamer. The nucleic acid aptamer has a nucleic acid sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The invention also provides a lateral flow chromatography detection test strip for alpha-calasodine. The lateral flow chromatography detection test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the conjugate pad is sprayed with a gold-labeled probe containing the aptamer and gold nanoparticles; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is fixedly provided with a first capture molecule, the first capture molecule is streptavidin, the quality control line is fixedly provided with a second capture molecule, and the second capture molecule is a conjugate of a streptavidin-biotinylated aptamer. According to the nucleic acid aptamer, the gold-labeled probe and the test strip disclosed by the invention, the detection efficiency of the alpha-camarin detection method is greatly improved, and the on-site instant demand is met.

The present invention discloses a pine wood nematode disease detection test strip and its preparation method and application, and relates to the field of molecular biology detection technology. The preparation method of the pine wood nematode disease detection test strip disclosed in the present invention comprises the following steps: preparation of haptens, preparation of immunogens and coatings, preparation of monoclonal antibodies, preparation of gold-labeled antibodies, and preparation of colloidal gold test strips; the amino acid sequence of the monoclonal antibody is shown in SEQ ID NO.1. The results of the embodiments of the present invention show that the monoclonal antibodies used in the test strips prepared by the present invention have high sensitivity; the test strips prepared by using colloidal gold-labeled monoclonal antibodies shorten the detection time of pine wood nematode disease, optimize the overall detection process, and provide good technical support for further promoting the early detection of pine wood nematode disease.

The invention discloses a method for detecting lactoferrin in milk based on a competition method test strip, which comprises the following steps: coupling heparin molecules with bovine serum albumin to obtain a heparin molecule-bovine serum albumin artificial antigen; modifying gold nanoparticles by using an artificial antigen to form a gold label I, and constructing a competition method test strip by matching with a gold label II modified by mouse IgG (Immunoglobulin G); a milk sample is subjected to centrifugal fat removal, pH adjustment and casein precipitation pretreatment and then dropwise added to the test strip, and the LF concentration is calculated through a T line gray value and a working curve. Detection can be completed within about 10-15 min, specificity is high (interference of impure protein is avoided), on-site rapid quantitative / semi-quantitative detection of LF in milk can be achieved, the antiviral activity of LF can be indirectly reflected through the binding strength of heparin and LF, the method is suitable for production quality inspection, dealer sampling inspection and consumer self-inspection, and the detection sensitivity reaches 1 microgram / mL.

The invention belongs to the technical field of biological material structure and performance analysis, and discloses a rapid qualitative and quantitative detection kit for a collagen triple-helix structure and a detection method. The detection kit comprises an enzymolysis system solution A of a non-triple helix structure of specific enzymolysis collagen; an ultrafiltration centrifugal tube; the enzymolysis system liquid B is used for performing non-specific enzymolysis on small-molecule polypeptide; colloidal gold test paper; the colloidal gold test paper is sequentially provided with a sample pad, a combination pad, an NC membrane and a water absorption pad from left to right; the NC membrane is sequentially coated with a T line and a C line from left to right; the marker on the combination pad is a gold-labeled antibody prepared from a hydroxyproline monoclonal antibody and colloidal gold particles through electrostatic coupling; and a coating substance on the T line is a hydroxyproline-carrier protein conjugate. The detection kit provided by the invention realizes a rapid, simple and good-specificity qualitative and quantitative detection method for the collagen triple-helix structure.

The invention relates to the field of tumor treatment instruments, and discloses a miniature gold label implant and forming equipment thereof, an implant body is cylindrical, and a plurality of wing plates are arranged on the side wall of the implant body; the equipment comprises a forming assembly and a feeding assembly, wherein the forming assembly comprises a base, a limiting disc arranged on the base, a limiting groove formed in the limiting disc and a cutter arranged in the limiting groove; the miniature gold label implant can be placed in tumor tissue and then identified and positioned to the marker body by a radiotherapy instrument, so that the radiotherapy instrument can accurately position the position of the tumor, the wing plates are designed into barbs, the implant can be prevented from shifting, the two ends of the gold label are designed into a blunt round shape and a sharp shape, and the stability is enhanced by utilizing tissue wrapping difference; the coating is arranged on the side wall of the bottom of the wing plate, the wing plate expands when encountering tissue fluid in the tissue, and the tumor tissue is physically clamped; meanwhile, forming equipment is provided, the side wall of the gold-labeled raw material alloy wire harness can be machined, and a wing plate structure is cut out.

The application discloses a kind of for HBV PreS1 antibody qualitative detection test strip and its preparation method and application, it is related to virology, immunology, biological detection technology and immunochromatography analysis technical field.The test strip is based on the specific recognition principle of antigen and antibody, the specific binding of PreS1 antigen and PreS1 antibody is realized, the rapid detection of PreS1 antibody in serum or plasma sample, it is sequentially assembled by sample pad, gold mark pad, nitrocellulose membrane (NC membrane) and water absorption pad, wherein, gold mark pad is fixed with colloidal gold labeled anti-human IgG antibody as gold mark probe, NC membrane detection line (T line) is coated HBV PreS1 antigen, for capturing PreS1 antibody in sample, NC membrane quality control line (C line) is coated HB2 antibody, for judging whether the test strip detection process is effective.The test strip in the application does not need complex instrument equipment, detection process is simple, fast and fast, and has good sensitivity and specificity, can be used for hepatitis B vaccine immune effect evaluation and large-scale screening.

The present invention relates to the field of biological detection, and particularly to a copper deposition enhanced lateral flow immunoassay detection kit, a detection method and an application thereof. The detection method first obtains a gold-labeled antibody; mixes a sample to be detected with the gold-labeled antibody to form an antigen-antibody complex solution, and drops the antigen-antibody complex solution onto a lateral flow immunoassay test strip for color development; immerses the lateral flow immunoassay test strip dropped with the antigen-antibody complex solution into a mixed solution containing CuSO4 and ascorbic acid, adds sodium dichloroisocyanurate for reaction; and quantitatively analyzes the concentration of a target analyte according to the color development intensity on the test strip. The present invention also relates to a lateral flow immunoassay detection kit and its application. The detection method can achieve detection in complex matrix samples and detection of low-concentration target analytes.

The invention is applicable to the technical field of animal virus detection, and provides a PVC2 and PRRSV (porcine reproductive and respiratory syndrome virus) double colloidal gold test strip and application thereof. Ferritin nanocage fusion protein of PCV2 and PRRSV is taken as a capture antibody; a gold-labeled PCV2 monoclonal antibody and a gold-labeled PRRSV monoclonal antibody are used as detection antibodies, and the gold-labeled PCV2 monoclonal antibody and the gold-labeled PRRSV monoclonal antibody are obtained by labeling a specific monoclonal antibody composition by using a gold labeling technology and treating the specific monoclonal antibody composition. The prepared double colloidal gold test strip is high in specificity, low in production cost, easy to operate and visual in result, PCV2 and PRRSV infection can be rapidly identified and diagnosed on site at the same time, and the double colloidal gold test strip has wide market application prospects.

1. The name of the design product: packing bottle (red dog gold mark series). 2. The use of the design product: the design product is used for container. 3. The design points of the design product: in shape. 4. The picture or photo that can best show the design points: perspective view 1.

1.The name of the design product: packaging jar (6.05 kg special gold label soy sauce). 2.The use of the design product: the design is used for food packaging. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.