279 results about "Gold labelling" patented technology

The invention discloses a colloidal gold kit for jointly detecting coronavirus IgM/IgG antibody, and a preparation method thereof, and relates to the field of biological medicine. Whether anti-novel coronavirus nucleocapsid protein IgM antibody and/or anti-novel coronavirus nucleocapsid protein IgG antibody exists in human serum or plasma or not by adopting an antigen-antibody sandwich method anda colloidal gold immunochromatography method principle, the novel coronavirus nucleocapsid protein containing 6xHis mark is marked by applying colloidal gold, thereby forming gold-marked N protein tobe adsorbed on a gold-marked pad, the novel coronavirus nucleocapsid protein containing 6xHis mark is used as an indication marker, the mouse-anti-human u chain monoclonal antibody is coated on the IgM detection line of a NC membrane, the mouse-anti-human IgG monoclonal antibody is coated on the IgG detection line and the mouse-anti 6xHis monoclonal antibody is coated on a quality control line ofthe NC membrane, the qualitative detection of the anti-novel coronavirus nucleocapsid protein IgG antibody is realized, and the colloidal gold kit disclosed by the invention has the advantages of being convenient to use, high in sensitivity and short in detection time.

The present invention belongs to the field of biological detection. Multi-line immunochromatographic test strip for semi-quantitative detection of aflatoxin B₁ comprises a paperboard, wherein a water-absorbing pad, a detection pad, a gold-labeled pad and a sample pad are adhered sequentially on one surface of the paperboard from top to bottom, wherein each adjacent pads is overlapped and connected, the detection pad uses a nitrocellulose film as a backing pad, the nitrocellulose film is provided with a transverse control line, a test line I, a test line II and a test line III, wherein the control line is coated with a rabbit anti-mouse polyclonal antibody, and the test line I, test line II and test line III are coated with aflatoxin B₁-bovine serum albumin conjugate (AFB₁-BSA), respectively: and the gold-labeled pad is transversely coated with a nanogold-labeled anti-aflatoxin B₁ monoclonal antibody. Said test strip is used for semi-quantitative detection of aflatoxin B₁, and is characterized by quick detection, simple procedure and high sensitivity.

The invention discloses a probe capable of specifically identifying bisphenol A nucleic acid aptamer and test strip detection application of the probe. The probe has a sequence shown in SEQID NO.1, and can be applied to the preparation of a bisphenol A immunochromatographic gold-labeled test strip, and the like; the test strip comprises a lining plate; at least four sticking objects including a sample film, a nucleic acid aptamer probe-gold-labeled conjugate pad, an enveloping film provided with a detection line and a reference line, and a water absorption film are sequentially stuck on the lining plate, wherein the nucleic acid aptamer probe-gold-labeled conjugate pad comprises compound of a nucleic acid aptamer probe and gold nanoparticles. Due to the test strip, the rapid qualitative and quantitative determination of bisphenol A can be realized by direct observation without using other auxiliary instruments and equipment, the detection work can be rapidly completed, and the accurate detection result can be obtained; the probe capable of specifically identifying the bisphenol A nucleic acid aptamer has the characteristics of being high in specificity, good in stability, low in cost, easy to use and the like, and can be used for a hospital, a family, the customhouse with responsibility of food safety inspection and quarantine, an airport, a port and the like which need a rapid field detection mechanism.

The invention discloses a colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and a preparation method. The test paper comprises a sample absorption area, a gold mark probe area, an immobilization antigen and antibody area, a water absorption area and a supporting plate, and the sample absorption area, the gold mark probe area, the immobilization antigen and antibody area and the water absorption area are laid on the supporting plate and are partially overlapped in sequence. The gold mark probe area is covered with gold mark probes which are mouse anti-swine IgG antibodies marked by colloidal gold. The immobilization antigen and antibody area is provided with a detection line T1 covered with pseudorabies virus gE protein, a detection line T2 covered with pseudorabies virus gB protein, a detection line T3 covered with pseudorabies virus gD protein and a control line C covered with goat anti-mouse IgG antibodies. The test paper is fast in detection, high in accuracy, strong in specificity, easy and convenient to carry and operate and capable of being used for differential diagnosis on PRV wild poisonous infection and vaccine immunity and evaluation on the PRV vaccine immunity effect at the same time.

A colloidal gold-silver united test paper used for detecting etiology of important disease on Felidae and Canidae animal is prepared mix-enveloping mouse source RV, CDV, RPV and CPV single-anti being labeled with colloidal gold on glass cellulose membrane to form combination pad; enveloping IgG of purified dog-resisting RV, CDV, FPV and CPV on R, D, F and P positions of detection line at nitric acid cellulose membrane and enveloping rabbit resisting mouse IgG on C position of quality control line at nitric acid cellulose membrane to from detection membrane.

The invention relates to a preparation method of a piece of human H-FABP colloidal gold test paper. The preparation method comprises the steps of preparing colloidal gold particles, preparing gold labelling antibody, preparing a gold labelling combination mat, coating a nitrocellulose membrane, preparing a sample processing mat, assembling the test paper and the like. According to the preparation method of the human H-FABP colloidal gold test paper, by designing a buffer solution system in the preparation process of the test paper, the problems that the detection time is long, the specificity is poor, the cost is high, and the like in the existing test paper can be solved. The invention also discloses a piece of human H-FABP colloidal gold test paper and application thereof.

The invention discloses a simple method for detecting HLA-G and antibody thereof by gold-labeled immunoassay. The invention uses the monoclonal antibody of HLA-G and an HLA-G antigen fragment and/or HLA-G for successfully developing a gold-labeled immunity percolation analysis kit, and a gold-labeled immunochromatography sandwich method and competition method kit, which are used for the public to easily, rapidly and cheaply self-check and self-test the HLA-G in body fluid such as blood, saliva, urine, and the like so as to diagnose the early malignant tumor. In order to diagnose the earlier malignant tumor, a kit for detecting the HLA-G antibody by a gold-labeled immunochromatography indirect method is also developed and used for diagnosing the earlier malignant tumor. The kits are all called as 'broad-spectrum tumor diagnostic kit'. The invention aims at developing an easy, rapid and cheap 'broad-spectrum tumor diagnostic kit' for the self-check and self-test of the public so as to realize primary tumor screening and achieve the goal of finding and curing the tumor early, save the lives of the tumor patients, and reduce the tumor mortality.

The invention discloses a gold-labeled chromatographic detection test paper card for brown meat essence multi-residue analysis, and the test paper card comprises a plastic support and a test paper core, wherein the test paper core is formed by sequentially bonding a sample pad, a gold-labeled material combination pad, a chromatographic film and a water absorption pad onto a lining board; the gold-labeled material combination pad is glass fiber cotton adsorbed with 2-5 mixed gold-labeled antibodies; the gold-labeled antibodies are colloidal-gold-labeled monoclonal antibodies or polyclonal antibodies for resisting brown meat essence; detection lines are 2-5 corresponding brown meat essence-carrier protein conjugated antigen strips; control lines are formed by printing of goat or rabbit anti-mouse IgG, goat anti-rabbit IgG or rabbit anti-carrier protein IgG antibodies; and the detection lines and the control lines are arranged in parallel in the transverse direction along the chromatographic film. The test paper card has high specificity and sensitivity, and the minimum detection limit can be 1ppb, thereby realizing fast detection of brown meat essence multi-residue gold-labeled chromatography. By using the test paper card, multiple brown meat essence residues can be simultaneously detected after one-step sample application, thereby greatly saving the detection time, improving the working efficiency and saving the detection cost.

The invention belongs to the field of biological detection. An AFM1 immunity chromatography test paper strip is characterized in that: the test paper strip comprises a paperboard, an absorbent pad, a detection pad, a colloidal gold pad and a sample pad are sequentially pasted on one side of the paperboard, and adjacent pads overlappingly connect at a joint; the detection pad treats a cellulose nitrate membrane as a base pad, a quality control wire and a detection wire are arranged on the cellulose nitrate membrane in a top-down manner, the detection wire is coated with an AFM1-bovine serum albumin (AFM1-BSA) conjugate, and the quality control wire is coated with a rabbit-anti-mouse polyclonal antibody; and the colloidal gold pad is transversely sprayed with a nanogold labeled AFM1 monoclonal antibody which is generated by a hybridoma cell strain 2C9 with the accession number of CCTCCNO.C201018. The test paper strip which is used for AFM1 detection has the characteristics of rapid detection, simple operation and high sensitivity.

The invention relates to the field of zoonosis immune diagnosis and discloses a brucellosis antibody detection immunochromatography test strip based on colloidal gold as a marking material and a preparation method of the test strip. In a quick brucellosis antibody detection technology, the 40-nm colloidal gold labeled with staphylococcus aureus protein A (SPA) is sprayed on glass fibers to form a gold labeled pad. Genes OMP31 and BP26 are cloned from a brucella genome, form prokaryotic expression recombinant plasmids and are transformed in escherichia coli to express proteins omp31 and bp26, the two proteins as coating antigens are respectively coated on a nitrocellulose membrane to serve as detection lines, and the detection lines, the gold labeled pad, a specially treated sample loading pad and water absorption paper are assembled into an immunochromatography detection device. The test strip has the characteristics of strong specificity, high sensitivity, convenience, simplicity, economy and the like, can be applied to typing detection of brucellosis antibodies of sheep and cattle, and has very important meaning and practical application value for brucellosis monitoring and prevalence control.

The invention relates to a test paper strip for rapidly detecting traces of chlorothalonil and a preparation method thereof. The test paper strip is characterized in that a supporting layer is used as the bottom layer, an adsorption layer is used as the intermediate layer, a protective layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fibrous layer, a gold-labeled antibody fibrous layer, a cellulose film and an absorbent material layer at the handle end in order from a test terminal, wherein the cellulose film is provided with detection blots printed by using a chlorothalonil coupling carrier protein solution and control blots printed by using a goat anti-mouse IgG or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody solution; the gold-labeled antibody is a colloidal gold labeled chlorothalonil monoclonal antibody or polyclonal antibody; and the chlorothalonil coupling carrier protein is bovine serum albumin, chicken ovalbumin or haemocyanin. The test paper strip disclosed herein has the advantages of strong specificity, high sensitivity, simple, and accurate detection, low cost, wide application scope, and easiness in popularization and application.

The invention discloses an anti-avian leukosis virus p27 protein monoclonal antibody, a gold-colloidal strip containing the same, and application. The anti-avian leukosis virus p27 protein monoclonal antibody is generated by secretion of a hybridoma cell strain with a preservation number of CGMCC NO. 11089 or a hybridoma cell strain with a preservation number of CGMCC NO. 11090. The monoclonal antibody is high in titer to p27 protein and good in specificity, and therefore can be used for preparing a kit and the gold-colloidal strip for detecting an avian leukosis virus. A preparation method of the monoclonal antibody provided by the invention is simple, and an antibody purification process is simple, and the efficiency is high, and the cost is low. As the gold-colloidal strip prepared by the anti-avian leukosis virus p27 protein monoclonal antibody provided by the invention is adopted to detect the anti-avian leukosis virus, the specificity is strong, and the operation is simple, and convenience, speediness and simplicity are realized, besides, a special instrument and equipment are not needed, and professional training is not needed, and a result is clear and easy to recognize; the operation is simple and convenient; the popularization is easy, and the antibody is more suitable for real-time detection.

The invention belongs to the field of clinical laboratory techniques and in particular relates to a colloidal gold test strip for quantitatively detecting human bone metabolic biomarker and a detection device applying same. The test strip provided by the invention comprises a sample pad, a glass fiber mat coated with gold-labeled streptavidin, a glass fiber mat coated with a gold-labeled antibody, a nitrocellulose membrane and an absorbent pad which are sequentially adhered with one another on a bottom plate in a staggered manner. A quality control line pre-enveloped with a goat anti-rat IgG antibody is formed in the nitrocellulose membrane; a detection line parallel to the quality control line is formed in the nitrocellulose membrane; an antibody capable of being specifically bound with a to-be-detected antigen human bone metabolic biomarker is coated on the detection line; the gold-labeled antibody is an antibody capable of being specifically bound with the to-be-detected antigen human bone metabolic biomarker. According to the test strip provided by the invention, the human bone metabolic biomarker can be quantitatively detected, the requirement on instruments is low, the time of clinically detecting the human bone metabolic biomarker is shortened, and the detection complexity and cost can be reduced.

The invention discloses an ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof used for the problem of fast detection for pregnancy, comprising a piece of detecting test paper and urine sample treatment solution; wherein, the detecting test paper consists of a base plate, a cellulose nitrate membrane, a gold label anti-Beta HCG monoclone antibody layer, an Alpha-HCG monoclone antibody line, a sheep anti-rat polyclonal antibody line, an absorbing pad and a sample pad; the matching composition of the urine sample treatment solution is that: 12.5 to 18.0 portions of disodium hydrogen phosphate dodecahydrate, 1.2 to 1.7 portions of sodium dihydrogen phosphate dihydrate, 40.0 to 48.0 portions of sodium chloride and 4600 to 5300 portions of distilled water; the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method of the invention are characterized by fast and simple detection, the operation of blood-drawing detection for 2 to 6 hours can be replaced by 2 to 3 minutes with time-saving, manual-saving and cost-saving; besides, the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof have the advantages of detecting ectopic pregnancy and pregnant days in a semiquantitative way, etc., and can be used for external detection of the pregnant condition and artificial abortion and assisted diagnosis.

The invention provides an immune colloidal gold test card for testing high-risk type human papillomavirus. A test strip thereof consists of a back lining, a sample pad, a combining pad, an NC membrane and a water-absorbing pad; the upper middle part of the back lining is provided with the NC membrane; the two ends of the NC membrane are respectively connected with the combining pad and water-absorbing pad in a neighboring way; the sample pad is arranged above the combining pad; a gold-labelled antibody G-Ab1 is sprayed on the combining pad; the coated gold-labelled antibody G-Ab1 is the monoclonal antibody of the high-risk type human papillomaviru virus HPV16; and the NC membrane is respectively sprayed with the polyclonal antibody Ab2 of the high-risk type human papillomaviru virus HPV16 and the antibody of the gold-labelled antibody, namely the IgG antibody of a rabbit antimouse. The invention applies the technology of immune colloidal gold into the quick testing of the high-risk type human papillomaviru virus; moreover, the test strip for quick testing the high-risk type human papillomaviru virus does not need a test device, thus being convenient for the application to the clinic quick diagnosis and suitable to hospitals of various grades to screen cervical carcinoma.

The nano CGIA test strip and testing method for bladder carcinoma combines ICG method and nano CG technique. Wherein, it is prepared by: overlaying and sticking on the all of an absorption-urine glass fiber layer, a polyester film layer, a glass fiber layer fixed gold-labeled baseboard anti-CK19 antibody or anti-Ks19.2 antibody, a CN film layer with a T testing zone enveloped said antibody and a C mass control zone enveloped sheep antibody and rat antibody, and an absorption-water filter paper layer.

The invention provides a method for quickly detecting Newcastle disease virus. The method comprises the following steps that: a Newcastle disease virus monoclonal antibody, a goat-anti-Newcastle disease virus polyclonal antibody and a goat-anti-Balb/C mouse immunoglobulin antibody are established; the treatment of a combination pad and NC membrane is completed; after a to-be-detected sample is added on a sample pad and filtered, the NDV in the sample is combined with the gold-labeled antibody in the combination pad; the gold-labeled antibody is firstly combined with the Ab2 on the NC membrane through chromatography to form a visible combining line, and an uncombined gold-labeled antibody undergoes chromatography continuously and is combined with Ab3 to form a second combining line; finally, a step of result observation, during which, two combining lines are formed when the result is positive, while one quality control line is formed when the result is negative. The method has the characteristics of simple operation, quickness, high sensitivity and excellent specificity, etc.; moreover, the method is low in cost and is suitable for Newcastle disease virus detection at grass root or clinic; in addition, the method can detect the Newcastle disease virus in bird blood and dejection and can obtain a result within 10 min with the sensitivity reaching to 10ng/ml. The invention also provides a Newcastle disease immune colloidal gold test card for realizing the method.

The invention discloses a semiquantitative detection system for luteinizing hormone and a detection method thereof. The system is composed of a test strip reading meter and a colloidal-gold test strip. The test strip reading meter can semiquantitatively detect the color depth of a detection line on the colloidal-gold test strip of regular molecules and total molecules of the luteinizing hormone, test results within a period of time are drawn into a curve on an LCD of a detector, the peak period of the luteinizing hormone is prompted, and the ovulation time is accurately judged. The test strip reading meter comprises a power switch, a single chip microcomputer, a motor, a light emitting diode, a photoelectric sensor, a power amplifier, an A/D converter, the LCD, a storer and a key. Visual testing is not needed, detection results are more accurate and are not affected by the intensity of ambient light and vision, testing is automatic and accurate, the color depth records can be drawn into a curve on the LCD on the detector, and therefore one ovulation period is judged.

The invention discloses a gold-labeled test strip for rapid detection of chromium ions as well as a preparation method and an application thereof. The gold-labeled test strip is characterized in that a bottom layer is a support layer, a middle layer is an absorption layer, a protection film is fixed on the absorption layer, the absorption layer is sequentially provided with a sample pad, a gold-labeled antibody binding pad, and a cellulose membrane layer from a test end as well as a water absorption pad at a handle end, detection prints printed by a carrier protein solution coupled with the chromium ions are arranged on the cellulose membrane layer, and contrast prints printed by rabbit-anti-mouse or goat-anti-mouse IgG (Intravenous Gamma Globulin) antibody solution are arranged on the cellulose membrane layer; colloidal gold-labeled chromium ion monoclonal antibodies are coated in the gold-labeled antibody binding pad. The gold-labeled test strip can be used for rapidly detecting pollution residues of the chromium ions in soil, water and food, has the advantages of specificity, sensitivity, rapidness, simplicity, convenience, visual and intuitional result and the like, not only can be used for screening large-batch samples, but also can be used for rapidly detecting small-batch samples and is wide in applicable range.

The invention discloses an immunochromatography test strip for detecting cystic echinococcosis and a preparation method thereof. The test strip comprises a hemofiltration membrane sample pad, a gold-marking pad, a cellulose membrane and a water absorption pad, wherein the gold-marking pad is tightly connected with the hemofiltration membrane sample pad and comprises an antibody labeling colloid gold probe of anti-echinococcus antigen advantage special antibody subtypes, the cellulose membrane is tightly connected with the gold-marking pad, the water absorption pad is tightly connected with the other end of the cellulose membrane, a detection line and a quality control line are arranged on the cellulose membrane, the detection line comprises crude antigens aiming at the cystic echinococcosis, and the quality control line comprises anti-antibodies capable of realizing special combination with the antibodies of the anti-echinococcus antigen advantage special antibody subtypes. The immunochromatography test strip of the invention has the advantages of simplicity, convenience, sensitivity, specificity and high speed, and is applicable to clinical and field use.

The invention relates to the field of biomedicine, and specifically relates to an enterovirus 71 antigen detection test strip (colloidal gold method) and a preparation method and application thereof. Enterovirus 71 can cause hand-foot-and-mouth disease, which has largegeneration proportion of severe infections (viral encephalitis, meningomyelitis virus and pulmonary edema), and a high death rate reaching 10%-25%. The test strip of the invention is used for rapid diagnosis of EV(enterovirus)71 infection. A virus separation and an RT-PCR (reverse transcription-polymerase chain reaction) are methods first used for EV71 antigen detection, but are not suitable for primary clinic usage due to defects of difficult operation and high costs, etc. The invention overcomes the above insufficiencies and provides a reagent, which is highly demanded in clinic detection, simply operated, suitable for various medical disease control sections, and capable of detecting EV71 antigens in human oropharyngeal swabs, bubble liquid, serum or excrement, and also provides the preparation method and application thereof. A technical scheme is as follows: a specimen is dropped on a sample pad, and the EV71 antigen wherein combines with a gold-labeled EV71 polyclonal antibody in a gold-labeled pad and migrates along a chromatography membrane. A detected line captures colloidal gold particles to form a red line visible to naked eyes, so as to realize detection of the EV71 antigen.

The invention discloses application of SARS-COV-2 Spike protein in detection of 2019 novel coronavirus. According to the invention, the HEK293 cell strain capable of stably and highly expressing the S1 protein subunit recombinant protein of the SARS-COV-2 Spike protein is obtained, and the S1 protein subunit recombinant protein of the SARS-COV-2 Spike protein is applied to the development of an immunochromatographic detection reagent. According to the invention, the SARS-COV-2 Spike protein S1 protein subunit recombinant antigen is used as a marking material and is applied to a gold-labeled immunochromatography system; the detection system adopts direct labeling capture, and compared with an IgM/IgG detection product taking prokaryotic expression SARS-COV-2 NP recombinant protein as a coating, the SARS-COV-2 Spike protein have greatly improved sensitivity and specificity.

The invention relates to a device for detecting samples and in particular relates to a test strip for detecting samples. The test strip for detecting samples comprises a gold-labeled pad and a detection pad, and also comprises a gold-labeled buffer pad. Because the gold-labeled buffer pad exists in the test strip, the sample is fully reacted with an antibody. Meanwhile, an outer adhesive sticker is separated from the inner gold-labeled pad, so that a phenomenon that heights of two sides of the gold-labeled pad are different is avoided or the difference between heights of two sides of the gold-labeled pad is reduced in a test strip production process, and the speeds of the samples flowing through the gold-labeled pad are the same. Therefore, analytes in the samples can be fully combined with antibodies with gold labels. Finally, the phenomena of breakage of a test structure line and color difference are effectively reduced.

The invention discloses a porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatograohic assay test strip as well as a preparation method and application thereof. The test strip comprises a sample absorption region, a colloidal gold labeled probe region, a solidifying antibody region, a water absorption region, a bottom plate and a clamping shell, wherein the sample absorption region, the colloidal gold labeled probe region, the solidifying antibody region and the water absorption region are sequentially bonded on the bottom plate and are interlapped in the clamping shell; the sample absorption region is coated with purified porcine pseudorabies virus gE protein; the colloidal gold labeled probe region is coated with colloidal gold labeled porcine pseudorabies virus gE protein monoclonal antibody; the solidifying antibody region is sequentially provided with a detection line T coated with mouse anti-pig IgM monoclonal antibody and a control line C coated with goat anti-mouse IgG. The test strip is simple to operate, good in repeatability, high in sensitivity, rapid and intuitive in result, simple in process and low in cost, can be mass-prepared, and is suitable for mass field test and early diagnosis for porcine pseudorabies virus infection in swinery.