145 results about "Immunoglobulin G" patented technology

Provided herein are novel immunoglobulin G (IgG) proteases and fusion proteins comprising the same. The IgG proteases find utility in methods of treating diseases mediated in whole or in part by pathogenic immunoglobulin G (IgG) antibodies, including methods for preventing or treating antibody-mediated rejection (AMR) of an organ allograft in an organ-transplant patient.

The invention provides a test strip for rapidly detecting methicillin-resistant staphylococcus aureus and application of the test strip, and belongs to the technical field of biochemical detection. The test strip comprises a bottom plate, a sample pad, a combination pad, a detection pad and a water absorption pad, the two ends of the detection pad are in lap joint with the combination pad and the water absorption pad respectively, the detection pad is located on the lower portion, the sample pad is in lap joint with the combination pad, and the combination pad is located on the lower portion; a detection line and a quality control line are arranged on the detection pad, the detection line is arranged on one side close to the combination pad, and the quality control line is arranged on one side close to the water absorption pad; the detection pad is coated with a MoS2 nanosheet, the combination pad is coated with a penicillin binding protein 2a polyclonal antibody labeled by horse radish peroxidase, and the detection line is coated with a penicillin binding protein 2a monoclonal antibody; the quality control line is coated with goat anti-rabbit IgG (immunoglobulin G). The kit is high in sensitivity and wide in linear range, has good specificity, repeatability and stability, and can effectively detect MRSA in a clinical sample.

The invention relates to a line elimination method immunochromatography test paper, which is formed by bonding a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad on a bottom plate in a mutual overlapping manner in sequence, and the nitrocellulose membrane is provided with a C line for contrast and a T line for detection, a colloidal gold compound marked by streptavidin and a colloidal gold compound marked by rabbit IgG (immunoglobulin G) are sprayed on the combination pad; a T line on the nitrocellulose membrane is coated with an FITC labeled antibody, and a C line on the nitrocellulose membrane is coated with anti-IgG. The line elimination method immunochromatography test paper can eliminate lines when the cutting proportion of the probe reaches 50%, so that the detection sensitivity is greatly improved, and the line elimination method immunochromatography test paper can be applied to target gene detection products in a CRISPR (clustered regularly interspaced short palindromic repeats) mode, including genotyping of pathogenic microorganisms, SNP (Single Nucleotide Polymorphism), insertion and deletion, structural variation, methylation modification site detection or drug resistance gene identification.

The invention discloses a method for constructing an N-glycosylation spectrum type biological age evaluation model based on plasma immunoglobulin G and application of the biological age evaluation model. Based on a glycosyl quantitative result, a clinical biochemical detection index, a lifestyle measurement index, a multi-dimensional health score and the like, a glycosyl metabolic index, a glycosyl inflammation index, a glycosyl cardiovascular index, a glycosyl stroke index, a glycosyl liver index and a glycosyl kidney index are fitted. And inputting the obtained different glycosyl data into the model, and speculating the aging process and health risk of each organ system of the detected person according to the risk threshold in the early-stage crowd. The method can predict a'glycosyl biological age system 'which can reflect health level and disease risk better than'calendar age', has individual specificity, is very sensitive to change of inflammatory response in vivo, is relatively stable in a short time, can be used as an index for early detection and screening of senescence, and can be used as an index for early detection and screening of senescence. And thus, effective reference is provided for individualized health suggestions of the detected personnel.

A method for administration of an IgG preparation by an intradermal (ID) route to a subject includes loading with a volume of the IgG preparation an ID delivery device including needles, applying the device to a skin delivery site, using the device to allow dermal penetration of the needles, delivering the volume of the IgG preparation at the skin delivery site, and removing the injection delivery device. The method can be used in the treatment of a disease, such as an immunodeficiency.

Fusion polypeptides are provided and comprise at least two ligand binding domains and a fragment crystallizable (Fc) region of immunoglobulin G (IgG). The ligand binding domains include an amino acid sequence selected from the group consisting of SEQ ID NOS: 1-25 and / or one of the at least two ligand binding domains binds modulates B cell activity while the other modulates T cell activity. Isolated nucleic acids, vectors, and isolated cells encoding or including the fusion peptides are further provided. Pharmaceutical compositions include the fusion peptides and a pharmaceutically-acceptable vehicle, carrier, or excipient. Methods of treating Sjögren's Syndrome and / or Systemic Lupus Erythematosus are also provided and comprise administering to a subject in need thereof the fusion polypeptide including the two ligand binding domains and the Fc region of IgG.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention relates to the technical field of biological detection, in particular to a sample diluent as well as a preparation method and application thereof. The sample diluent comprises a dilution component, and the dilution component comprises the following raw materials in percentage by mass: 0.005%-0.02% of thermally denatured immunoglobulin G, 0.5%-2.0% of a protein protective agent, 0.5%-1.5% of a metal ion chelating agent, 0.1%-0.5% of an ampholytic surfactant and 0.5%-2.0% of a sugar alcohol compound. According to the sample diluent, through the interaction of all the components, the ability of the sample diluent to reduce the matrix effect of serum and plasma samples can be remarkably improved, so that the interference of non-specific reaction and matrix effect of serum and plasma on the detection process is reduced, and the accuracy of serum and plasma sample detection can be improved; and a more reliable and accurate experiment result can be obtained.

Described herein are bifunctional compounds for removing macrophage migration inhibitory factor (MIF) or immunoglobulin G (IgG). Further described herein are pharmaceutical compositions comprising these bifunctional compounds. Further described herein are methods for treating pathologies and / or conditions with said compounds or compositions. The pathologies and / or conditions are mediated through MIF / IgG or where MIF / IgG is a contributing factor in the development and perpetuation of diseases and / or conditions, such as autoimmune diseases and cancers, among others. TIFF2025509736000504.tif99145

The present disclosure relates to a method for purifying an immunoglobulin degrading enzyme (IdeS) from Streptococcus pyogenes, preferably for removing IdeS charge variants and IdeS obtainable from said method.

Provided is a method of treating rheumatoid arthritis, comprising administering to a subject in need of such treatment a therapeutically effective amount of a degrader of immunoglobulin G and an anti-arthritis agent. Also provided is a pharmaceutical composition comprising a degrader of immunoglobulin G and an anti-arthritis agent.

The present application relates to the field of livestock breeding technology, and discloses a molecular marker related to the level of natural immunoglobulin G antibody in chicken plasma and application thereof.The molecular marker related to the level of natural immunoglobulin G antibody in chicken plasma is the 251th nucleotide in SEQ ID NO:1, and the polymorphic form is G or A.The SNP molecular marker related to the level of natural immunoglobulin G antibody in chicken plasma provided by the present application can be used to select the level of natural immunoglobulin G antibody in chicken plasma at the genetic level, directly screen the level of natural immunoglobulin G antibody in chicken plasma from the root source, accurately grasp the genetic information, effectively avoid the disadvantages of traditional methods, significantly improve the efficiency and accuracy of selection, and can be applied to chicken breeding practice on a large scale, and promote the development of the industry.

The invention discloses an application of a biomarker in evaluating the risk of progressing from autoimmune hepatitis to cirrhosis. The biomarker comprises immune globulin G, and / or an antinuclear antibody, and / or aspartate aminotransferase and alanine aminotransferase. Independent predictive factor indexes of the liver cirrhosis risk of an autoimmune hepatitis patient in the biomarker are as follows: an antinuclear antibody ANA index: log2ANA is greater than or equal to 1.62; the AAR index of the ratio of aspartate aminotransferase to alanine aminotransferase is as follows: AAR is greater than or equal to 1.62; the immune globulin G, namely the IgG index, is greater than or equal to 30.3 g / L. The problems that in the prior art, an AIH risk model does not take cirrhosis as the outcome, visual presentation is lacked, prediction efficiency is insufficient, and clinical early-stage accurate risk layering and diagnosis and treatment decision requirements are difficult to meet are solved.

This invention provides a bovine colostrum IgG nutritional supplement gummies and their preparation method, belonging to the field of functional food technology. The bovine colostrum IgG nutritional supplement gummies of this invention, through precise formulation of specific components, effectively improve the dispersion uniformity of whole-fat bovine colostrum freeze-dried powder in the gummy base, significantly retaining the activity of immunoglobulin G, giving the product a stable texture and good taste; further, combined with a special process adapted to the formula, high yield and high raw material utilization are achieved in industrial production. This product combines immune nutritional supplementation with convenient consumption, making it suitable for people of all ages and possessing good market application prospects.

This invention relates to the fields of biomedical technology and biological model construction technology, and discloses an ANCA-associated vasculitis glomerular endothelial cell injury model, its construction method and application, and the application of BACH1 inhibitors. The method includes: (1) activating neutrophils sequentially with tumor necrosis factor α and immunoglobulin G to obtain a culture medium containing activated neutrophils; wherein the immunoglobulin G is immunoglobulin G isolated and purified from the plasma of patients with ANCA-associated vasculitis; (2) co-culturing the culture medium containing activated neutrophils with human glomerular endothelial cells to obtain the cell injury model. The cell injury model provided by this invention can verify the role of neutrophil adhesion characteristics in endothelial cell injury, and at the same time, the cell injury model can better simulate the immune microenvironment of ANCA-associated vasculitis glomerular endothelial cells in vivo.

Described herein are bifunctional compounds for removal of macrophage migration inhibiting factor (MIF) or immunoglobulin G (IgG). Further described herein are pharmaceutical compositions comprising these bifunctional compounds. Further described herein are methods of treating disease states and / or conditions with the compounds or the compositions. The disease state and / or condition is mediated by MIF / IgG or wherein MIF / IgG is an onset factor for the progression and persistence of diseases and / or conditions such as autoimmune diseases and cancer.

The present disclosure relates to a method for separating and obtaining a target protein-enriched solution, such as immunoglobulin G (IgG), and a plurality of target protein-depleted fractions from a protein mixture, such as plasma or fractions thereof, using continuous chromatography. The present disclosure also relates to a continuous chromatography system for separating a target protein from a protein mixture, a formulation of its purified product and uses.

The application relates to the field of biological medicine, and particularly discloses application of saliva TP17, TP47 and TP15 specific antibodies in preparation of an auxiliary diagnostic product for neurosyphilis. Based on specific antibody immunoglobulin G (IgG) of Treponema pallidum in saliva, combined detection of saliva TP17 / IgG, TP47 / IgG and TP15 / IgG outputs a risk value of neurosyphilis of a subject, or a four combined Logistic regression discriminant model is constructed by combining serum TRUST titers, and the subject is stratified into a low-risk or high-risk group based on a preset judgment threshold, so that the comprehensive recognition capability for neurosyphilis is improved. The saliva is used as a detection sample, and the application has the advantages of non-invasiveness, convenience, easy popularization, repeatable collection and the like, and can be used for early screening, auxiliary diagnosis and clinical path management of neurosyphilis.

The present invention belongs to the field of optical sensing technology and discloses an optical sensor based on a rare-earth-doped microbubble cavity. To predict concentration, the rare-earth-doped microbubble cavity is first pumped with a pump source laser. An immunoglobulin G solution to be tested is then injected into the microfluidic channel of the rare-earth-doped microbubble cavity, and the resulting signal laser spectrum is recorded. The signal laser power is then compared with a standard curve that correlates concentration and signal laser power. The concentration corresponding to the same signal laser power is found, and the concentration of the immunoglobulin G solution to be tested is thus determined. By utilizing the active cavity structure of the rare-earth-doped microbubble cavity to detect biomolecules, the present invention enables the realization of a high-precision biosensor capable of predicting both the concentration of an immunoglobulin solution and the occurrence of specific binding between multiple immunoglobulins.

Provided herein are engineered fusion proteins comprising ApoM (e.g., human or murine ApoM) fused to a constant region (Fc) of a immunoglobulin G (IgG, e.g., human IgG or murine IgG). In some embodiments, the ApoM-Fc fusion protein further comprises a signal peptide (e.g., IL-2 signal peptide) fused to the ApoM, allowing the fusion protein to be secreted once expressed recombinantly. Methods of using the ApoM-Fc fusion protein or the sponge variants in the treatment of various diseases or disorders are provided.

The invention discloses a creatine kinase isoenzyme detection reagent, and belongs to the technical field of biological detection. The creatine kinase isoenzyme detection reagent comprises a reagent A and a reagent B, the reagent A is prepared from 30 to 60mM of a Hepes buffer solution, 0.2 to 0.5 g / L of mouse IgG (Immunoglobulin G), 1 to 5g / L of TritonX-100, 0.4 to 0.8 g / L of a stabilizer and 1 to 2g / L of a template separating agent; the reagent B is prepared from 30 to 60 mM of Hepes buffer solution, 1 to 3 g / L of sealing agent, 1 to 5 g / L of stabilizing agent and 1 to 3 g / L of polystyrene latex microsphere coupled with antibody. The template separating agent is added into the reagent A, so that active sites of CK-BB or CK-MM can be effectively hindered, the influence on CK-MB is reduced by selecting the reagent, and combination of CK-BB or CK-MM and a template is facilitated; after the polystyrene latex microspheres added with the magnetic coupling antibody in the reagent B are combined with the CK-MM, the content of an object to be detected is determined according to the change of the absorbance value of a sample, and the creatine kinase isoenzyme detection reagent is high in accuracy.

The utility model discloses a kit for ultra-sensitive detection of a PLA2R-IgG (Immunoglobulin G) antibody. The kit comprises a kit body, and a box cover door is arranged on the front side of the kit body and hinged to one end of the kit body; a foldable handle is arranged on the box body; rotary connecting pieces are fixedly mounted on the outer surfaces of the left and right sides of the box body; the two ends of the foldable handle are rotatably and movably connected with the box body through rotary connecting pieces respectively; a sliding rail with a groove is fixedly mounted at each of the upper part and the bottom part of the inner walls of the left side and the right side of the box body, and the sliding rail at the upper part is connected with the transverse box; a rectangular positioning groove capable of placing a polystyrene pore plate is formed in the transverse box; the bottom slide rail is connected with the bottom box; and the bottom box is provided with a circular positioning hole groove for placing a reagent bottle for containing a reagent or a standard substance and a rectangular positioning hole groove for placing an ice bag. By arranging the sliding rail, the transverse box, the bottom box, the positioning hole groove, the foldable handle and the like, the purpose of improving convenience and stability in the storage and transfer process is achieved.

The present invention discloses a monoclonal antibody cell strain 6F6 for camel immunoglobulin G and its application. The 6F6 strain has been deposited in the General Microbiology Center CGMCC of the China Microbiological Culture Collection Administration Committee, and its address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. It is classified and named as a monoclonal antibody cell strain of camel immunoglobulin G (IgG), with a deposit date of August 9, 2023, and a deposit number of CGMCC No.45650. Through artificial expression, purification, immunization and screening of camel IgG protein, a monoclonal antibody cell strain 6F6 that can stably and efficiently express camel IgG was obtained. It was identified as the IgG1 subtype, and the recognition epitope was KEPPSMRLKARPG. It has been successfully applied to the antibody detection of camel brucellosis, and the established method has good sensitivity and specificity.

The present invention relates to a multivalent, preferably bivalent, antibody complex, more preferably a diabody, i.e. a bivalent scFv, capable of recognizing and binding the human protein CD99, a composition comprising said complex and the use thereof for diagnostic purposes and / or for the treatment and / or the follow-up of leukaemias and / or myelodysplastic syndromes. Furthermore, the present invention relates to antibodies, preferably monoclonal, of the immunoglobulins G group type capable of recognizing and binding the human protein CD99, a composition comprising said immunoglobulins and the medical use thereof, in particular for the treatment and / or the follow-up of cancer, preferably solid tumours that express CD99 and / or leukaemias and / or myelodysplastic syndromes.

The invention discloses a kit for latex immunoturbidimetric determination of urine immunoglobulin G, and relates to the field of biological detection technology. The kit includes R1 reagent and R2 reagent; the R1 reagent includes 10-200mM tris(hydroxymethyl)aminomethane, 1-10g / L bovine serum albumin, 10-50g / L reaction enhancer, 0.1-10mL / L preservative, 100-300mM NaCl, 2-6g / L surfactant; the R2 reagent includes 10-200mM tris(hydroxymethyl)aminomethane, 100-300mM NaCl, 1-5g / L antibody-coupled latex particles, 1-5g / L glycerol, 5-10g / L methyl-β-cyclodextrin, 1-10g / L bovine serum albumin, 2-6g / L surfactant and 0.1-10mL / L preservative. The kit of the present invention has good repeatability and stability.

Provided herein are aptamers for binding immunoglobulin G (IgG) and methods of detecting IgG in a sample. The aptamers may be used for detecting the levels of IgG in a biological sample. Also provided herein is a sensor attached an aptamer for the detection of IgG.

The invention discloses a double-antibody sandwich ELISA kit for detecting ENTV and JSRV and application of the double-antibody sandwich ELISA kit, and belongs to the technical field of biology. The double-antibody sandwich ELISA kit contains an ELISA plate coated with a monoclonal antibody for resisting goat endemic intranasal tumor virus p27 protein, a polyclonal antibody for resisting goat endemic intranasal tumor virus p27 protein and HRP (horse radish peroxidase) labeled goat anti-rabbit IgG (immunoglobulin G). Wherein the monoclonal antibody for resisting the goat endemic intranasal tumor virus p27 protein is secreted by a hybridoma cell strain ENTV-2C3, the hybridoma cell strain ENTV-2C3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: C 2024412. The double-antibody sandwich ELISA kit established by the invention has the characteristics of high detection sensitivity and good specificity, can be used for detecting goat endemic intranasal tumor virus and sheep lung adenoma retrovirus, and provides a technical means for prevention and control of goat endemic intranasal tumor and sheep lung adenoma.

Provided herein are compounds, including compounds of any of the formulae described herein (e.g., Formula (I'), Formula (I), Formula (II)), and pharmaceutically acceptable salts thereof. Compounds provided herein are capable of covalently binding to and inhibiting neonatal fragment crystallizable receptor (FcRn) proteins and are therefore useful in the treatment and / or prevention of various diseases, including conditions associated with FcRn activity and / or immunoglobulin G (IgG) autoantibody levels in a subject.

The invention belongs to the technical field of optical sensing, and discloses an optical sensor based on a rare earth doped microbubble cavity, which is characterized in that when the optical sensor is used for predicting the concentration, the rare earth doped microbubble cavity is pumped by utilizing pumping source laser; then injecting an immunoglobulin G solution to be detected into the microfluidic channel of the rare earth doped microbubble cavity, and recording to obtain a changed signal laser spectrum; and comparing the signal laser power with a standard curve of the relationship between the concentration and the signal laser power to find a concentration value corresponding to the same signal laser power, so as to obtain the concentration of the immunoglobulin G solution to be detected. Biomolecules are detected by using an active cavity structure of the rare earth doped microbubble cavity, so that a high-precision biosensor can be realized, the concentration of an immune globulin solution can be predicted, and whether specific binding occurs among various immune globulins can also be predicted.

The invention relates to the technical field of preparation of immune globulin G nanoparticles, in particular to an immune globulin G nanoparticle as well as a preparation method and application thereof. The invention discloses an immune globulin G nano-particle. The particle size of the immune globulin G nano-particle is 10 nm to 200 nm; the invention discloses an immunoglobulin G nano-particle as well as a preparation method and application thereof. The prepared immunoglobulin G nano-particle has better thermal stability, and the prepared immunoglobulin G nano-particle still keeps effectiveness at high temperature; compared with the traditional immune globulin G, the immune globulin G nanoparticles prepared by the method disclosed by the invention have the advantages that the half-life period is effectively prolonged, and frequent intake is not needed; the immunoglobulin G nano-particles prepared by the method disclosed by the invention have higher biological activity.