Immune colloidal gold test strip for detecting porcine epidemic diarrhea virus as well as preparation method and application thereof

A technology for porcine epidemic diarrhea and colloidal gold test paper, which is applied in the field of reagents and test strips for detecting porcine epidemic diarrhea virus by using colloidal gold immunochromatography technology, which can solve the problem of large error in results, long time required, complicated operation process, etc. problem, to achieve good detection stability, good detection effect, and good stability.
CN103454419AActive Publication Date: 2013-12-18NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2013-12-18

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Abstract

The invention discloses an immune colloidal gold test strip for detecting porcine epidemic diarrhea virus as well as a preparation method and application thereof. The test strip sequentially comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, an absorbent paper and a PVC (poly vinyl chloride) base plate arranged below and used as an assembling platform according to a connection sequence, wherein the colloid gold pad comprises a glass cellulose membrane of a PEDV (porcine epidemic diarrhea virus) monoclonal antibody adsorbed with colloidal gold marks, the nitrocellulose membrane is provided with a goat rat resisting IgG (immunoglobulin G) polyclonal antibody coated quality control line and a rabbit resisting PEDV S protein polyclonal antibody coated detection line, and the PEDV monoclonal antibody is generated in secretion of hybridoma cells with a preservation number CCTCC C201392. Experiments prove that the test strip has the advantages of strong specificity and good stability; the operation is simple, technicists do not need special training, no special equipment is needed, the detection cost is low, the detection speed is fast and the results can be read in 5-10 minutes, and the test strip is applicable to field test.
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Description

technical field

[0001] The invention relates to a reagent for detecting porcine epidemic diarrhea virus, in particular to a test strip for detecting porcine epidemic diarrhea virus by using a colloidal gold immunochromatography technique, and the invention belongs to the field of virus detection. Background technique

[0002] Epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED). It mainly harms piglets. Sick suckling piglets have watery diarrhea and vomiting symptoms. Newborn piglets often suffer from severe dehydration and death after infection. Weaned pigs and fattening pigs have watery diarrhea for 4 to 6 days after illness, and their growth and development are affected after recovery. Influenced by weight loss in the late fattening period. Due to the short course of the disease and rapid spread, the disease spreads widely in our country and countries all over the world, causing serious economic losses every year.

[0003] The main st...

Claims

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