Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
31results about How to "Avoid repeated freezing and thawing" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Freeze-drying protection system required for nucleic acid amplification reagent and preparation method of freeze-drying protection system
InactiveCN109593834AKeep aliveNo biohazardMicrobiological testing/measurementFreeze thawingFreeze-drying
The invention belongs to the technical field of medicine preparation, and particularly relates to a freeze-drying protection system required for a nucleic acid amplification reagent and a preparationmethod of the freeze-drying protection system. The freeze-drying protection system comprises the nucleic acid amplification reagent and a freeze-drying protection additive, wherein the nucleic acid amplification reagent is a reagent used for LAMP reaction amplification, the freeze-drying protection additive is one or a compound of the following reagents: polyethylene glycol, mannitol, polyvinylpyrrolidone, glucan, trehalose, sucrose, bovine serum albumin, collagen, threonine and glycine, and the concentration of the weight-to-volume ratio of the freeze-drying protection additive and the amplification reaction reagent is 3% to 25%. The freeze-drying protection additive used in the invention has the advantages of small volume, short freeze-drying time, high efficiency and low energy consumption, and the freeze-drying protection system can be directly used for gene chip experiments, does not cause repeated freeze-thaw and waste of reagents, and can effectively ensure the activity of active substances in the freeze-drying process.
Owner:百康芯(天津)生物科技有限公司
DNA (deoxyribonucleic acid) preserving solvent and DNA preserving method
The invention discloses a DNA (deoxyribonucleic acid) preserving solvent and a DNA preserving method. The DNA preserving solvent comprises glycerol, TE buffer solution and 1-carboxyl-N, N, N-trimethyl-B lactone, wherein the final volume of the glycerol is 25-80%, the final concentration of the TE buffer solution is 1*TE-5*TE, and the final concentration of the 1-carboxyl-N, N, N-trimethyl-B lactone is 1-6mol / L. The DNA preserving method comprises the following steps of: (1) extracting DNA; and (2) dissolving the DNA in the DNA preserving solvent to be preserved. The DNA preserving solvent disclosed by the invention has the advantages of simple preparation, low cost, good preserving effect and long shelf life and is prevented from freezing and thawing repeatedly. The invention provides initial DNA records for genomics research, identity authentication, gene diagnosis, gene therapy, organ transplantation and the like in the future.
Owner:SHANGHAI YUAJIRO BIOTECH
Double fluorescent lyophilized microchip, kit and method for detecting novel coronavirus 2019-nCoV
ActiveCN111334608ASensitive detectionImprove accuracyMicrobiological testing/measurementAgainst vector-borne diseasesReverse transcriptaseFreeze-drying
The invention discloses a double fluorescent lyophilized microchip, a kit and a method for the novel coronavirus (2019-nCoV). The kit comprises the lyophilized microchip, a tube of mineral oil, a tubeof positive control, a tube of negative control, a tube of diluent and a tube of nuclease-free water, wherein the lyophilized microchip is coated with a primer, a probe, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP, Mg<2+>. According to the lyophilizing condition, the temperature of an insulator is reduced to negative 55 DEG C at a pre-freezing stage; pre-freezing is kept for 1 hour, and equipment is used for vacuumizing, and freeze-drying is kept for 1 hour; in an analytic drying stage, the temperature of the insulator is increased to negative 25 DEG C and kept for 1 hour, isincreased to 37 DEG C and kept for 2 hours and is reduced to 25 DEG C and kept for 1 hour to obtain the lyophilized microchip, and the diluent and sample nucleic acid are added during use. The doublefluorescent lyophilized microchip can be used for simultaneously detecting an ORF 1a / b gene and an N gene. The detection kit and detection method have high accuracy, high specificity, high sensitivityand short detection time.
Owner:BEIJING YISEN BIOTECH
Freeze-dried microchip for identifying porcine reproductive and respiratory syndrome virus classic strain and NADC30-Like strain, kit and method thereof
ActiveCN110885900ASensitive detectionImprove accuracyMicrobiological testing/measurementAgainst vector-borne diseasesPorcine reproductive and respiratory syndrome virusFluorescent pcr
The invention discloses a freeze-dried microchip for identifying a porcine reproductive and respiratory syndrome virus classic strain and an NADC30-Like strain, a kit and a method thereof, which belong to the field of molecular detection. The freeze-dried microchip is characterized in that a fluorescent PCR reaction system used for identifying the porcine reproductive and respiratory syndrome virus classic strain and the NADC30-Like strain is fixed on the microchip through freeze-drying; wherein the fluorescent PCR reaction system comprises the following primers and probes: a porcine reproductive and respiratory syndrome virus classic strain upstream primer, a porcine reproductive and respiratory syndrome virus classic strain downstream primer and a porcine reproductive and respiratory syndrome virus classic strain Taqman probe sequence; an NADC30-Like strain upstream primer, an NADC30-Like strain downstream primer, and an NADC30-Like strain Taqman probe. According to the present invention, the porcine reproductive and respiratory syndrome classical strain virus and the NADC30-Like strain virus are simultaneously detected, and the detection kit has characteristics of high accuracy,high specificity, high sensitivity and short detection time.
Owner:CHINA ANIMAL DISEASE CONTROL CENT +1
Freeze-drying protective agent of SNP detection reagent, and application
PendingCN112626175AStable storageReduce shipping costsMicrobiological testing/measurementTrehaloseGelatin
The invention relates to a freeze-drying protective agent of an SNP detection reagent, and a protection method. The freeze-drying protective agent comprises trehalose, glucan and gelatin. The protective agent disclosed by the invention realizes a purpose of constant-temperature transportation and constant-temperature preservation of the SNP detection reagent, and transportation cost is dramatically lowered. The freeze-drying protective agent is simple to operate, and pollution brought by repeated freeze thawing of the reagent and repeated utilization of the SNP detection reagent can be avoided.
Owner:HANGZHOU KMB BIOTECH
Quick batch preparation method of cotton genome DNA (deoxyribonucleic acid) suitable for PCR (polymerase chain reaction)
InactiveCN102925430ASimple stepsThere are few types of useDNA preparationGenetically modified cropsReal-time polymerase chain reaction
The invention discloses a quick batch preparation method of cotton genome DNA (deoxyribonucleic acid) suitable for PCR (polymerase chain reaction), relating to the molecular detection of genetically modified crops in the field of crop breeding. The method comprises the following steps of: putting the tender leaves at the top of a cotton plant into a centrifuge tube; adding an extracting solution and steel balls; breaking the sample; performing treatment in a water bath; adding a leaching solution for leaching; precipitating DNA with isopropyl alcohol; washing with ethanol; and dissolving the precipitate in water to obtain the extracted DNA. The method disclosed by the invention solves the problems of complicated steps and time and labor consumption in cotton genome DNA extraction; the invention provides a method capable of quickly extracting the DNA meeting the requirements of PCR detection in batches; and the method can be applied to the molecular biological detection in the links of genetically modified cotton breeding, safety report and the like.
Owner:XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
NAT2 gene polymorphism fluorescence PCR melting curve detection kit
InactiveCN106367489ADetection time is shortShort timeMicrobiological testing/measurementFluorescenceNat2 gene
The invention discloses an NAT2 (N-acetyltransferase-2) gene polymorphism fluorescence PCR (Polymerase Chain Reaction) melting curve detection kit, which comprises a first probe, a second probe, a third probe, a fourth probe, a positive primer and a reverse primer, wherein different fluorescence groups are respectively marked at 5' ends of the first probe, the second probe, the third probe and the fourth probe; and corresponding quenching groups are marked at the 3' ends of the first probe, the second probe, the third probe and the fourth probe. The detection of four mononucleotide polymorphisms on four sites can be completed in a single-tube PCR system; the sample genotype can be known through once fluorescence PCR melting curve analysis after the PCR amplification is completed; the whole operation can be completed in 3 hours; and the time consumption is low.
Owner:XIAMEN ZEESAN BIOTECH +1
Automatic liquid nitrogen storage tank
PendingCN111637669AAvoid repeated freezing and thawingImprove storage qualityLighting and heating apparatusDead animal preservationStructural engineeringProcess engineering
The invention relates to an automatic liquid nitrogen storage tank. The automatic liquid nitrogen storage tank comprises a liquid nitrogen tank, a freezing storage mechanism and a lifting mechanism; atank port is formed in the top of the liquid nitrogen tank; the freezing storage mechanism comprises a plurality of freezing storage frames; the multiple freezing storage frames are evenly distributed in the liquid nitrogen tank with the center of the liquid nitrogen tank as a center of a circle, a plurality of placing plates are sequentially arranged on each freezing storage frame from top to bottom, cross-shaped placing grooves are correspondingly formed in the tops of the placing plates, a plurality of freeing storage boxes are correspondingly arranged in each cross-shaped placing groove in a cross-shaped distribution mode, and each freezing storage frame is connected with the liquid nitrogen tank through a first rotating mechanism; the lifting mechanism comprises a supporting frame; and a lifting plate is arranged on the supporting frame, the lifting plate is connected with the supporting frame through the lifting mechanism, and the bottom of the lifting plate is connected with the top of an installation plate through a second rotating mechanism. The automatic liquid nitrogen storage tank has the beneficial effects that the freezing storage boxes required by the liquid nitrogen tank can be accurately taken out, the degree of automation is high, the difficulty of manual operation is lowered, repeated freezing and thawing of samples is avoided, and the storage quality of thesamples is improved.
Owner:ZHONGNAN HOSPITAL OF WUHAN UNIV
Multiple fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit for streptococcus suis type 2 virulence gene and detection method thereof
InactiveCN112941211AAids in quality monitoringEnsure biosecurityMicrobiological testing/measurementMicroorganism based processesMultiplexBiological product
The invention relates to a multiplex fluorescent quantitative PCR detection kit for a streptococcus suis type 2 virulence gene and a detection method thereof. The multiplex fluorescent quantitative PCR detection kit mainly comprises an SS-2 primer probe mixed solution, a universal primer mixed solution, an SS-2 PCR reaction solution, an SS-2 primer probe mixed solution, an SS-2 negative control, an SS-2 positive control and an SS-2 quantitative standard substance. By adopting the kit, quantitative detection of extracellular factor epf, lysozyme release protein mrp and hemolysin sly genes of streptococcus suis type 2 virulence genes can be realized. The detection sensitivity of the kit is high, and the lowest detection limit is 1.0 * 10 < 3 > copies / mL; the quantitative linear range is wide, and three virulence genes of streptococcus suis type 2 of 1.0 * 10 < 3 > copies / mL-1.0 * 10 < 10 > copies / mL can be accurately quantified; the specificity of the kit is high, and no reaction with other bacteria and common porcine viruses exists; the repeatability is good, and the intra-batch and inter-batch variation coefficients are both smaller than 5%; the stability of the kit is high, the lowest detection limit reference of 1.0 * 10 < 3 > copies / mL can be detected by 100% after repeated freezing and thawing for 8 times, and the kit is very suitable for monitoring of conventional epidemic situations of swine diseases and detection requirements of biological products from swine blood on streptococcus suis.
Owner:HANBANG MEDICAL SCI & TECH HARBIN CITY
Magnetic low-temperature storage device capable of automatically storing and taking samples
PendingCN114655547AAvoid repeated freezing and thawingEnsure cryogenic safetyPackaging under vacuum/special atmosphereDischarging meansProcess engineeringCryopreservation
The invention relates to the field of biological sample storage, in particular to a magnetic low-temperature storage device capable of automatically storing and taking samples. The device comprises a liquid nitrogen tank, a rotary positioning assembly, a storage assembly, a sample storing and taking assembly and a control assembly, the device is used for keeping low temperature in the liquid nitrogen tank through liquid nitrogen, and a storing and taking opening is formed in the liquid nitrogen tank; the three storage discs are arranged in the liquid nitrogen preparation tank, the three storage discs are sequentially arranged in the liquid nitrogen tank at preset intervals from top to bottom, and access notches are formed in the other storage discs except the lowest storage disc in the three storage discs; the storing and taking notch is used for enabling a sample storing and taking assembly to penetrate through the storing disc, the storing disc is used for storing a cryopreservation tube cabin, and cryopreservation tubes are stored in the cryopreservation tube cabin; and a rotary positioning assembly. According to the low-temperature storage device, the different storage discs can rotate, the cryopreservation tube cabins of all layers can be flexibly taken through the notches in the storage discs, the problem caused by the fact that cryopreservation tubes are extracted from a liquid nitrogen tank and then exposed in the whole lifting basket is solved, repeated freezing and thawing of biological samples are avoided, and the cryopreservation efficiency is improved. The low-temperature safety of the biological sample is ensured.
Owner:河北省计划生育科学技术研究院
Fluorescent RT-PCR reagent and method for detecting influenza A virus, influenza B virus and coronavirus SARS-CoV-2
InactiveCN112410469AAvoid risk of failureAvoid temperatureMicrobiological testing/measurementMicroorganism based processesFreeze-dryingLate gene
The invention provides a freeze-dried type multiple fluorescent RT-PCR reagent and a method for detecting and distinguishing influenza A virus, influenza B virus and coronavirus SARS-CoV-2. Primers and probes of the fluorescent RT-PCR reagent for detecting the influenza A virus, the influenza B virus and the coronavirus SARS-CoV-2 provided by the invention are obtained by designing and screening based on the latest gene sequence information of the influenza A virus, the influenza B virus and the coronavirus SARS-CoV-2 in a public gene sequence database, and the reagent has good inclusivity, specificity and reaction performance. According to the reagent, a human RNase P nucleic acid fragment is used as a detection internal reference. In addition, the fluorescent RT-PCR detection reagent forthe influenza A virus, the influenza B virus and the coronavirus SARS-CoV-2 is prepared into a freeze-dried form capable of being stored at normal temperature through freeze-drying technology, thus avoiding the risk that the conventional liquid fluorescent PCR detection reagent is invalid due to the change of storage temperature or repeated freezing and thawing, and the transportation and management cost is reduced.
Owner:深圳市赛格诺生物科技有限公司
DNA (Deoxyribonucleic Acid) low-temperature storage solvent and storage method thereof
The invention discloses a DNA (Deoxyribonucleic Acid) low-temperature storage solvent and a storage method thereof. The DNA storage solvent comprises calf serum, glycerol, a TE buffer solution, dimethyl sulfoxide and glycine betaine, wherein the final volume of the calf serum is 0.1%-1%, the final volume of glycerol is 10%-24%, the final concentration of the dimethyl sulfoxide is 30%-50%, the final concentration of the glycine betaine is 1M-7M and the pH range of the DNA storage solvent is 6.0-8.0. The DNA storage method comprises the following steps of: (1), extracting DNA; and (2), storing the DNA in the DNA storage solvent for storage. According to the DNA low-temperature storage solvent and the storage method thereof disclosed by the invention, the operation is simple, the cost is low, the DNA storage effect is good, the storage period is long and multigelation can be avoided, so that the most perfect DNA is provided for the scientific research, the identity authentication and the medical diagnosis.
Owner:SHANG OUTDO BIOTECH CO LTD
Serum replacement for immune cell suspending culture
InactiveCN107304411AClear ingredientsLow in proteinCulture processBlood/immune system cellsLipid formationAdditive ingredient
The invention relates to a serum replacement for immune cell suspending culture. The serum replacement is prepared from medicinal grade human albumin, recombinant human transferrin, recombinant human insulin, lipid, trace elements, hormones, shearing force protector, resveratrol, D-glucosamine hydrochloride, R-848 and C3. The serum replacement can be used for preparing a serum-free culture medium conveniently by a certain process before being used with a basic culture medium, and the serum-free culture medium can be used for suspending culture of immune cells. The serum replacement has the advantages that (1) the content of protein ingredients can be furthest reduced due to replaceable small-molecular compound in the serum replacement, ingredients are definite, and uniformity is strong; (2) the serum replacement can be prepared by a conventional method, and stored at negative 80-negative 20 DEG C; and (3) the serum replacement can be subpackaged and stored, and can be conveniently mixed with a basic culture medium before use, so that repeated freezing and unfreezing of the culture medium can be avoided.
Owner:康思葆(北京)生物技术有限公司
Preparation method of poultry embryo egg extracting solution
PendingCN107348497AAvoid destructionAvoid inactivationCosmetic preparationsToilet preparationsSucroseSaccharum
The invention relates to a preparation method of a poultry embryo egg extracting solution, and belongs to the technical field of health-care foods. The preparation method comprises the following technology steps of selecting poultry fertilized eggs, preforming conventional surface cleaning treatment, and performing conventional incubation for 8-12 days; after poultry embryos are determined to grow normally, performing cleaning, and disinfecting the surfaces of eggshells; collecting all substances in the embryos and eggs, and performing crushing with a crusher for 1-5 minutes so as to obtain homogenate; adding cane sugar which is 20-50% of the homogenate to the crushed homogenate, enabling the cane sugar to dissolve, and performing transferring to an ice bath; performing crushing treatment on the homogenate, wherein the crushing treatment comprises ultrasonic treatment, French high-pressure extruding treatment and / or grinding treatment with glass beads; performing centrifugation treatment on the homogenate; subpacking supernatant obtained through centrifugation in a sealed container, and performing pasteurization; or performing separation treatment on the supernatant obtained through centrifugation by a chromatographic fractionation method to obtain different particular protein and performing subpacking; and performing preservation at 4 DEG C or minus 20 DEG C. According to the preparation method disclosed by the invention, high-activity growth stimulation factors, high-activity nerve growth factors, high-activity active polysaccharide and high-activity active polypeptide are extracted and reserved to the maximum extent.
Owner:蔡小敏
Skin externally-applied liquid state and solid state kreotoxin (liushengtai) composite nano emulsion and preparation thereof
InactiveCN101214200BAvoid wastingAvoid repeated freezing and thawingCosmetic preparationsToilet preparationsPolyethylene glycolMannitol
Owner:丁克祥
Concentrated anti-freeze alga polypeptide liquid and preparation method thereof
ActiveCN108070022AHigh cost of solutionHigh activityPeptide preparation methodsFermentationPhosphatePolysaccharide
The invention relates to a concentrated anti-freeze alga polypeptide liquid and a preparation method thereof, and belongs to the technical field of food processing. The concentrated anti-freeze alga polypeptide liquid is prepared from, by weight, 3-10 parts of concentrated anti-freeze laver liquid, 1-10 parts of marine polysaccharid, 0.2-0.5 part of phosphate and 30-100 parts of deionized water. New raw materials with a low cost are provided for anti-freeze polypeptide, and the extraction rate of anti-freeze polypeptide is increased to achieve industrialization and expand the application field.
Owner:HAIXIN FOODS
Preparation method of nucleic acid primer preserving fluid
PendingCN112941148AImprove stabilityReduce degradationMicrobiological testing/measurementThiomersalateBiology
The invention discloses a preparation method of a nucleic acid primer preserving fluid, which is characterized in that the preserving fluid is prepared from the following components in 1000 mL: 2 to 3 parts of thiomersalate and 0.2 to 0.4 part of 1MTris-HCL; 0.1 to 0.3 part of a 0.5 M metal chelating agent; an acidity regulator and a buffer solution; and the balance of pure water. The preparation method comprises the following steps: fully mixing tris (hydroxymethyl) aminomethane, disodium ethylene diamine tetraacetate and pure water until the mixture is completely dissolved to form a first mixture; adding an acid-base regulator into the first mixture obtained in the step 1, mixing, regulating the pH value to 8.0, then adding thiomersalate, and finally adding pure water to a constant volume of 100ml to obtain the nucleic acid preserving fluid. The nucleic acid preservation solution can improve the stability of nucleic acid in a solution and delay degradation of the nucleic acid, so that the preservation period of the nucleic acid in a liquid state can be prolonged. Meanwhile, repeated freeze-dissolution can be effectively avoided, the experiment operation steps are reduced, and the whole experiment process is shortened.
Owner:通用生物(安徽)股份有限公司
A kind of method that utilizes blueberry pomace to produce blueberry fruit cake
InactiveCN103892154BAvoid churnAvoid exposure to oxidationFood freezingFood thermal treatmentFreeze thawingFruit cake
The invention provides a method for developing fruit cakes by utilizing blueberry juice residual residues as a raw material. The method comprises the following steps: directly performing vacuum freezing chopping treatment on frozen blueberry residues without melting and pulping, accelerating the dissolution of active ingredients in a manner of combining ultrasonic emulsification treatment with proper heat treatment, wherein the manner replaces a traditional boiling process, performing high-pressure pasteurization, and finally cooling and molding. Part of macromolecular hydrophilic colloid is added before sterilization, and the gel strength and the shape maintenance are improved. The method is simple to operate, low in heat treatment strength and short in time, and repeated freeze thawing and high-strength heat treatment of a product are avoided. Therefore, the method has the advantages that the microbial pollution probability is low, the retention rate of the effective active ingredients is high, the sensory quality is excellent, the shape maintenance at the temperature of 37-42 DEG C is good and the like.
Owner:ZHEJIANG UNIV OF TECH +1
Screening method and application of bacillus subtilis proprotease 9 targeted binding protein
PendingCN114438075ASimple processLow costPeptide/protein ingredientsMetabolism disorderPhage displayed peptideBacilli
The invention discloses preparation and application of a bacillus subtilis proprotease 9 (PCSK9) targeted binding protein. The PCSK9 targeted binding protein is screened and prepared through a phage display peptide technology, and a convenient, efficient, stable and low-cost choice is provided for treatment and management of hypercholesteremia and atherosclerotic cardiovascular diseases.
Owner:刘博巽
Biobank tissue cryopreservation methods and biobank tissue cryopreservation tubes
ActiveCN105191909BStrong water absorptionAvoid repeated freezing and thawingDead animal preservationShock-sensitive articlesAntigenAnatomy
The invention provides a biological sample bank tissue cryopreservation method and a biological sample bank tissue cryopreservation tube. The freezing method comprises: placing the biological sample bank tissue on the surface of a carrier sheet; The slices are placed in the tissue cryopreservation tube of the biobank, and the tissue cryopreservation tube of the biobank is sealed and stored at a low temperature; the cryopreservation tube includes a tube body, a cap and at least one for holding the separated biobank tissue. The carrier sheet can be detachably placed in the tube body; this cryopreservation method can solve the problem that the tissue of the biological sample bank is not easy to be identified or taken out because it is pasted on the inner wall of the cryopreservation tube after cryopreservation. It can also avoid repeated freezing and thawing of biological sample bank tissue when making frozen sections, thereby benefiting the protection of biological sample bank tissue quality (for example, biological macromolecules such as RNA, protein, antigen and antibody contained).
Owner:FUDAN UNIV SHANGHAI CANCER CENT
Pharmaceutical composition containing animal bone polypeptide
ActiveCN102920994BImprove securityImprove stabilityPowder deliveryPeptide/protein ingredientsMedicineStock solution
The invention relates to a bone polypeptide product, and in particular relates to a pharmaceutical composition containing animal bone polypeptide. The pharmaceutical composition comprises the following components in parts by weight: 100 parts of polypeptide injection stock solution extracted from pig bones, cow bones and deer bones, 0.1-5 parts of chloretone and 0.5-5 parts of sodium dihydrogen phosphate. The pharmaceutical composition containing animal bone polypeptide is good in stability, extremely low in high polymer content, high in safety and extremely suitable for clinic application.
Owner:罗诚
Freezing method of biological tissue sample and biological tissue freezing container
ActiveCN105383805BStrong water absorptionQuick take outDead animal preservationShock-sensitive articlesAntigenEngineering
The invention provides a cryopreserving method for a biological tissue sample and a biological tissue cryopreserving container. The cryopreserving method comprises the steps that the biological tissue is arranged on the surface of a bearing piece, the bearing piece to which the biological tissue is attached is placed in the biological tissue cryopreserving container in the long axis direction of the biological tissue cryopreserving container, and the biological tissue cryopreserving container is sealed and frozen at the low temperature; and the cryopreserving container comprises a container body, a sealing cover and at least on bearing piece used for bearing the separated biological tissue and separably arranged in the container body. According to the cryopreserving method, the problems that due to the fact that the biological tissue is prone to being cryopreserving on the inner wall of the biological tissue cryopreserving container, the biological tissue is not prone to being recognized or being taken out or being damaged after being taken out can be solved, the situation of multigelation when the biological tissue is made into cryopreserved sections can be avoided, and the protection of the sample quality (such as the contained RNA, proteins, antigens, antibodies and other biomacromolecules) of the biological tissue is facilitated.
Owner:FUDAN UNIV SHANGHAI CANCER CENT
Cryopreservation method for biological sample library tissues and biological sample library tissue cryopreservation tube
ActiveCN105191909ADamage reductionStrong water absorptionDead animal preservationShock-sensitive articlesAntigenBiological macromolecule
Owner:FUNDAN UNIVERSITY SHANGHAI CANER CENTER
Freeze-dried microchip for identifying H9 and H6 subtype low-pathogenicity avian influenza viruses, kit and method thereof
ActiveCN110885903ASensitive detectionImprove accuracyMicrobiological testing/measurementMicroorganism based processesMolecular biologyFreeze dry
The invention discloses a freeze-dried microchip for identifying H9 and H6 subtype low-pathogenicity avian influenza viruses, a kit and a method thereof, which belong to the field of molecular detection. The freeze-dried microchip is characterized in that a fluorescent PCR reaction system used for identifying H9 and H6 subtype low-pathogenicity avian influenza viruses is fixed on the microchip through freeze drying; wherein the fluorescent PCR reaction system comprises the following primers and probes: an avian influenza H9 subtype virus upstream primer, an avian influenza H9 subtype virus downstream primer and an avian influenza H9 subtype virus Taqman probe; an avian influenza H6 subtype virus primer, an avian influenza H6 subtype virus upstream primer, an avian influenza H6 subtype virus downstream primer and an avian influenza H6 subtype virus Taqman probe. According to the invention, H9 and H6 subtype low-pathogenicity avian influenza viruses are simultaneously detected, and the detection method of the detection kit is high in accuracy, specificity and sensitivity and short in detection time.
Owner:BEIJING YISEN BIOTECH +1
Polypeptide vaccine, preparation method and application
InactiveCN111558035AAvoid pollutionLess destructiveLavatory sanitoryCancer antigen ingredientsPenicillinSide chain
The invention belongs to the technical field of vaccines, and discloses a preparation method and application of a polypeptide vaccine. The preparation method comprises the following steps: respectively cleaning a packaging material penicillin bottle body, a bottle cap and a bottle plug, and sterilizing; preparing a mixed solution of normal saline containing 0.5% DMSO and DMSO as a solvent, and oscillating and uniformly mixing the solvent for later use; adding the solvent into a polypeptide freeze-dried powder, blowing and beating for 10 times, and uniformly mixing; sterilizing and filtering anobtained polypeptide-containing solution, and sub-packaging a filtered solution into the cleaned penicillin bottles. According to the method, microorganisms and residual endotoxin in the penicillin bottles are removed through dry heat sterilization, and bottle caps and bottle plugs are sterilized through high-temperature and high-pressure moist heat sterilization. According to the invention, a solvent which has little damage to polypeptide, can prevent side chain oxidation and can dissolve polypeptide is used. The polypeptide with the concentration prepared by the invention is stored at -80 DEG C, is stable in property and can be stored for a long time. The process provided by the invention is suitable for preparing personalized customized vaccines and meets the sterile and safety requirements specified in pharmacopeia.
Owner:浙江格源致臻生物医药科技有限公司 +1
Automatic Access Control Device for Frozen Cells
ActiveCN105379706BAutomatic access guaranteeImprove access efficiencyDead animal preservationControl systemCryopreservation
Owner:SHANGHAI ORIGINCELL BIOLOGICAL CRYO EQUIP CO LTD
Method for extracting mammary tissue RNA
The invention provides a pretreatment method for extracting mammary tissue RNA. The method comprises the steps that a mammary tissue frozen section material is prepared first, mammary gland tissue in the section material is indentified through HE staining, and the mammary gland tissue is cut off. The mammary gland tissue is directly used for cell lysis and RNA extraction, and then the high-quality and high-concentration RNA can be obtained. By means of the method, the problem can be solved that the mammary tissue for extracting the RNA is scarce to cause low RNA extracting quality.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
Dual fluorescence freeze-dried microchip, kit and method for detecting novel coronavirus 2019-ncov
ActiveCN111334608BSensitive detectionImprove accuracyMicrobiological testing/measurementAgainst vector-borne diseasesBiotechnologyPositive control
The invention discloses a novel coronavirus (2019‑nCoV) double fluorescence freeze-dried microchip, a kit and a method, the kit including a freeze-dried microchip, a tube of mineral oil, a tube of positive control, a tube of negative control, A tube of diluent and a tube of nuclease-free water, the lyophilized microchip is coated with primers, probes, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP, Mg 2+ . The freeze-drying conditions are as follows: in the pre-freezing stage, the temperature of the clapboard drops to -55°C, and the holding time is 1h during pre-freezing, and then the equipment is vacuumed to keep freeze-drying for 1h; in the analytical drying stage, the temperature of the clapboard is raised to -25°C Keep it at ℃ for 1h, then raise the temperature of the separator to 37℃ and keep it for 2h, and finally lower the temperature of the separator to 25℃ and keep it for 1h to obtain a freeze-dried microchip, just add diluent and sample nucleic acid when using it. The invention detects ORF 1a / b gene and N gene simultaneously, and the detection method of the detection kit has high accuracy, specificity and sensitivity, and the detection time is short.
Owner:BEIJING YISEN BIOTECH
Economic and practical method for extracting and separating bone tissue protein
InactiveCN106749604AIncrease concentrationGuaranteed purityPeptide preparation methodsAnimals/human peptidesBiologyPre cooling
The invention discloses an economic and practical method for extracting and separating bone tissue protein and belongs to the technical field of molecular biology. The method concretely comprises the following steps: placing a weighted centrifugal tube onto ice and pre-cooling; taking a phosphatase inhibitor, a protease inhibitor and phenylmethylsulfonyl fluoride, adding the three into a splitting buffer solution, and then placing the mixture onto the ice and uniformly stirring; weighting 0.3-0.5g of femoral shaft; putting the femoral shaft into a grinding kettle filled with liquid nitrogen and grinding the femoral shaft into powder; transferring the femoral shaft to a spare centrifugal tube and weighting, thereby obtaining the weight of the femoral shaft; multiplying the mass by 1 / 3, and then adding the lysate into the centrifugal tube in concentration of 0.2-0.4g / mL; shaking the mixed solution under a low temperature and then standing; placing the turbid liquid under the conditions of 4 DEG C and 3000-5000rpm and centrifuging; and absorbing the supernate to a new centrifugal tube and then split-charging and storing. According to the invention, the extraction and separation process is simple and the separated protein purity is high, so that the extraction and separation process is suitable for popularization and application in the field of biology.
Owner:NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
A kind of seaweed antifreeze polypeptide concentrate and its preparation method
ActiveCN108070022BHigh cost of solutionHigh activityPeptide preparation methodsFermentationBiotechnologyPorphyra
The invention relates to a concentrated anti-freeze alga polypeptide liquid and a preparation method thereof, and belongs to the technical field of food processing. The concentrated anti-freeze alga polypeptide liquid is prepared from, by weight, 3-10 parts of concentrated anti-freeze laver liquid, 1-10 parts of marine polysaccharid, 0.2-0.5 part of phosphate and 30-100 parts of deionized water. New raw materials with a low cost are provided for anti-freeze polypeptide, and the extraction rate of anti-freeze polypeptide is increased to achieve industrialization and expand the application field.
Owner:HAIXIN FOODS
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com