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Cryopreservation method for biological sample library tissues and biological sample library tissue cryopreservation tube

A technology for biological samples and cryopreservation methods, which is applied in the preservation, transportation and packaging of human or animal bodies, special packaging objects, etc., can solve problems such as difficult identification and removal, and achieve the effect of reducing damage

Active Publication Date: 2015-12-30
FUNDAN UNIVERSITY SHANGHAI CANER CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems, the main purpose of the present invention is to provide a method for cryopreservation of biological sample bank tissue, which can solve the problems caused by the adhesion of biological sample bank tissue on the inner wall of t

Method used

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  • Cryopreservation method for biological sample library tissues and biological sample library tissue cryopreservation tube
  • Cryopreservation method for biological sample library tissues and biological sample library tissue cryopreservation tube
  • Cryopreservation method for biological sample library tissues and biological sample library tissue cryopreservation tube

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Embodiment one

[0045] A method for freezing of biological sample bank tissue, comprising the steps of:

[0046] (1), separating the required biological tissue from the living body;

[0047] (2), processing the freshly separated biological tissue into a suitable size, placing it on the carrier sheet as a biological sample bank tissue, so that the biological sample bank tissue adheres to the surface of the carrier sheet;

[0048] (3), placing the carrier sheet adhered to the biological sample bank tissue in the biological sample bank tissue cryopreservation tube;

[0049] (4) Seal the tissue cryopreservation tube of the biological sample bank, and place it in a suitable direction at a low temperature for cryopreservation.

[0050]Wherein, in step (1), the biological tissue can be various human tissues or animal tissues, including surgically removed tissues and experimental animal tissues. According to the needs of the experiment, these biological tissues can be tumor...

Example Embodiment

[0055] Embodiment two

[0056] like figure 1 As shown, this embodiment provides a tissue cryopreservation tube 1 for a biological sample bank, which includes: a tube body 2 , a tube cap 3 and a carrier sheet 4 .

[0057] The tube body 2 is in the shape of a hollow cylinder, and is detachably connected with the tube cover 3 . When the tube body 2 and the tube cover 3 are connected together, the two form a closed space to prevent external substances (such as cryopreservation liquid, etc.) from entering the space during freezing, thereby avoiding the space from being polluted.

[0058] The tube body 2 and the tube cover 3 can be connected by various connection methods such as threaded connection and buckle connection. If the pipe body 2 and the pipe cover 3 are threaded to form a sealed connection, then an external thread can be established on the outer surface of the pipe body 2, and an internal thread can be established on the inner surface of the pipe cover 3, and the pipe b...

Example Embodiment

[0068] Embodiment Three

[0069] In the biological sample bank tissue cryopreservation tube in Example 2, only one biological sample bank tissue can be frozen in each tube. On the one hand, when a large number of biobank tissues need to be cryopreserved, an equal number of biobank tissue cryopreservation tubes are required, which may increase the cost of cryopreservation; During cryosectioning, it is necessary to continuously open the tissue cryopreservation tubes of the biological sample bank to take out the frozen tissues, which is time-consuming and laborious, which is not conducive to improving the efficiency of cryosectioning.

[0070] In order to solve the above problems, the tissue cryopreservation tube of the biological sample bank in this embodiment has been improved in structure, so that one biological sample bank tissue cryopreservation tube can freeze multiple tissues at the same time, so that when making frozen sections, just close the Or open the cryopreservatio...

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Abstract

The invention provides a cryopreservation method for biological sample library tissues and a biological sample library tissue cryopreservation tube. The cryopreservation method comprises the following steps: putting the biological sample library tissues onto the surface of a wafer; putting the wafer to which the biological sample library tissues adhere into the biological sample library tissue cryopreservation tube; sealing the biological sample library tissue cryopreservation tube and cryopreserving at a low temperature. The cryopreservation tube comprises a tube body, a tube cap and at least one wafer for bearing separated biological sample library tissues, wherein the wafer is arranged in the tube body separately. Through adoption of the cryopreservation method, the problem that the biological sample library tissues are difficult to identify or extract since the biological sample library tissues adhere to the inner wall of the cryopreservation tube after cryopreservation can be solved, and the biological sample library tissues can be prevented from being frozen repeatedly during preparation of frozen sections, so that protection of the quality of the biological sample library tissues (contained biological macromolecules such as RNA, proteins and antigen-antibodies) is facilitated.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and relates to a biological tissue cryopreservation method and a biological tissue cryopreservation tube. Background technique [0002] Biobank is a biological application system that centrally preserves various tissue materials of organisms and uses them for clinical treatment of diseases and life science research. Biobanks include tissue banks (such as normal tissues, tumor tissues, etc.), organ banks, cell banks, etc. [0003] In order to conduct in-depth research on the structural or biochemical properties of biobank tissues, long-term cryopreservation (such as storage in liquid nitrogen) is often required for biobank tissues, and they can be re-frozen at different times after cryopreservation. Conduct research on these biological tissues. Cryotubes are commonly used today for cryopreservation of biobank tissue. [0004] In order to make the cryopreservation tube not only maintain ...

Claims

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Application Information

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IPC IPC(8): A01N1/02B65D81/18
Inventor 杜祥
Owner FUNDAN UNIVERSITY SHANGHAI CANER CENTER
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