Method for extracting mammary tissue RNA

Abstract

The invention provides a pretreatment method for extracting mammary tissue RNA. The method comprises the steps that a mammary tissue frozen section material is prepared first, mammary gland tissue in the section material is indentified through HE staining, and the mammary gland tissue is cut off. The mammary gland tissue is directly used for cell lysis and RNA extraction, and then the high-quality and high-concentration RNA can be obtained. By means of the method, the problem can be solved that the mammary tissue for extracting the RNA is scarce to cause low RNA extracting quality.

Description

technical field [0001] The invention relates to the field of RNA extraction and purification. Background technique [0002] With the development of modern medicine, biomarkers have gradually become important reference indicators for disease diagnosis and treatment. [0003] In the detection of breast cancer, it is necessary to extract and detect RNA in cancerous and normal breast glandular tissues (rather than adipose and connective tissues). Cancerous breast glandular tissue is relatively easy to obtain, but normal breast glandular tissue is scarce. The reason is: breast cancer patients who need breast-conserving surgery have less normal breast tissue, and it is impossible to sample a large number of them; and cases of total mammectomy and neoadjuvant chemotherapy are rare. [0004] Normal mammary gland tissue for RNA extraction is scarce, and people often obtain low-quality and low-concentration RNA using conventional RNA extraction methods, and sometimes even no RNA can...

AI Technical Summary

Problems solved by technology

[0004] Normal mammary gland tissue for RNA extraction is scarce, and people often get low-quality and low-concentration RNA using conventional RNA extraction methods, and sometimes even no RNA can be extracted

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